Application of rhizoma polygonati and fructus lycii aqueous extract compound in preparation of product for improving immune injury diseases caused by chemotherapy drugs

Abstract

The invention discloses application of a rhizoma polygonati and fructus lycii water extract compound in preparation of a product for improving immune injury diseases caused by chemotherapeutic drugs, and belongs to the technical field of biological medicines. The rhizoma polygonati and fructus lycii water extract compound can improve and enhance functional damage of macrophages and T cells caused by chemotherapy drugs by inhibiting immune cell apoptosis, can be used as a functional additive to be added into immunocompetence health care products for use, and is used for adjuvant therapy and daily health care of chemotherapy patients. Through research and exploration of a scientific ratio, the rhizoma polygonati and fructus lycii water extract compound can inhibit chemotherapy drug induced immune cell apoptosis, provides a modern scientific basis for traditional application of rhizoma polygonati and fructus lycii, and enhances physiological functions and overall health conditions of patients. Further verification by combining with zoology tests proves that the compound of the rhizoma polygonati aqueous extract and the fructus lycii aqueous extract can more remarkably enhance the immune function of immunodeficient mice, including more effectively inhibit immune cell apoptosis, compared with the single rhizoma polygonati aqueous extract or the single fructus lycii aqueous extract.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedicine, and specifically relates to an application of a polygonatum and wolfberry water extract compound in the preparation of a product for improving immune damage symptoms caused by chemotherapeutic drugs. Background Art

[0002] Chemotherapy is a treatment method widely used to treat various types of cancer. It is the abbreviation of chemotherapeutic drug therapy. It is a treatment method that uses chemical drugs to prevent the proliferation, infiltration, and metastasis of cancer cells until it eventually kills the cancer cells. It has been proven to effectively reduce tumor burden and inhibit tumor development.

[0003] Common chemotherapy drugs include cyclophosphamide, vinorelbine, dactinomycin, doxorubicin, cisplatin, etc. Since chemotherapy drugs are not very selective, they will inevitably damage normal cells in the human body while killing cancer cells, resulting in adverse drug reactions such as extreme fatigue, decreased appetite, nausea, vomiting, loss of appetite, hair loss, low fever, suppression of bone marrow hematopoietic function, bleeding and tendency to infection. Most of these chemotherapy drugs are non-specific and mainly exert cytotoxic effects by targeting rapidly dividing cells, which have a profound impact on both cancer cells and rapidly proliferating healthy cells. Therefore, the quality of life of patients during chemotherapy has been significantly reduced, and symptoms such as fatigue, insomnia, nausea and vomiting, and decreased resistance are common.

[0004] Abnormal immune system function is the main reason why most chemotherapy patients have decreased resistance, susceptibility to infection and relapse. The immune system is composed of a complex network of immune organs, immune cells and immune molecules, which work together to identify and eliminate foreign or abnormal cells, including tumor cells. Among them, immune cells are the smallest working units of the immune system, and they need to maintain the function of the entire immune system through continuous proliferation and differentiation. Therefore, while chemotherapy drugs kill tumor cells, they will inevitably cause damage to immune cells, leading to immunosuppression or imbalance. The existing chemotherapy adjuvant drugs with immune-enhancing activity mainly include: thymosin; transfer factor; glutamine; newborn bovine liver active peptide; levamisole; imiquimod; active ingredients of traditional Chinese medicine, etc.

[0005] The prior art discloses a Chinese medicine composition for chemotherapy adjuvant, which can alleviate the adverse reactions caused by chemotherapy to a certain extent. However, the formula is composed of more than 30 kinds of medicines, the cost is relatively high, the medicinal properties are too strong, and it is easy to cause deficiency and inability to be supplemented; and the decoction is taken directly with a poor taste and easy to stimulate vomiting, making it difficult to achieve a therapeutic effect; other preparation methods are complicated, not suitable for home use, have certain limitations, and the auxiliary effect is poor.

[0006] There are many products in the prior art that use polygonatum and wolfberry as medicines in formula preparation, but such reagents all use a large amount of medicinal flavors: for example, a health product or pharmaceutical composition for relieving fatigue and enhancing immunity, which is a dosage form prepared from raw materials with the following weight ratio: 1-45 parts of cordyceps militaris, 2.5-75 parts of polygonatum, 2-60 parts of wolfberry, 2-60 parts of raspberry, 2-75 parts of mulberry, 3-75 parts of longan pulp, 5-150 parts of yam, 2-75 parts of jujube, and 2-60 parts of hawthorn. Or the raw materials include: 30-60 parts of Astragalus, 9-18 parts of Angelica, 15-24 parts of Ligustrum lucidum, 15-24 parts of Lycium barbarum, 15-24 parts of Curculigo orchioides, 15-24 parts of Epimedium, 9-18 parts of Polygonatum, 15-24 parts of Jiao Shenqu, 15-24 parts of Jiao Hawthorn, 15-24 parts of Jiao Malt, 15-24 parts of Fried Atractylodes macrocephala, 9-18 parts of Tangerine peel, 9-18 parts of Poria, and 3-9 parts of Jujube. Granules can be prepared by conventional methods. During preparation, 14 raw materials are decocted twice with water, each time for 1.5 hours, the decoctions are combined, filtered, and the filtrate is concentrated into a clear paste with a relative density of 1.25-1.30 (60°C); dried under reduced pressure at 70°C, crushed into fine powder; then dextrin is added to mix and granulated; dried at 60°C, the granules are obtained. Or it is prepared from the following raw materials in parts by weight: 3-5 parts of ginseng flower, 8-15 parts of angelica, 3-5 parts of poria, 3-5 parts of astragalus, 3-5 parts of saffron, 3-5 parts of wolfberry, 3-5 parts of polygonum multiflorum, 3-5 parts of polygonatum, 1-3 parts of psoralea corylifolia, 1-3 parts of white peony root, 1-3 parts of oldenlandia diffusa, 1-3 parts of coix seed, 1-2 parts of august sedge, 3-5 parts of dendrobium, 2-3 parts of ophiopogon, 1-2 parts of amomum, 3-5 parts of chicken gizzard lining, and 1-3 parts of liquorice. The preparation method is water decoction.

[0007] A large number of prescriptions are disclosed above, but these prescription products require the use of dozens of drugs or even more medicinal flavors in corresponding dosage ratios to achieve the corresponding technical effects. There is no relevant technical disclosure indicating that only the combination of Polygonatum sibiricum and Lycium barbarum can improve and treat abnormal immune system functions. Summary of the invention

[0008] As traditional Chinese medicinal materials, polygonatum and wolfberry contain a variety of biologically active ingredients, which can have a positive effect on the immune system and have potential value in improving immunity and anti-apoptosis. Therefore, through the scientific proportion of the water extracts of polygonatum and wolfberry, the purpose of the present invention is to provide a polygonatum and wolfberry water extract compound for use in the preparation of a product for improving immune damage caused by chemotherapeutic drugs, and the product is applied with the polygonatum and wolfberry water extract compound as an active ingredient. The polygonatum and wolfberry water extract compound has the function of inhibiting the apoptosis of immune cells induced by chemotherapeutic drugs, provides a modern scientific basis for the traditional application of polygonatum and wolfberry, and can provide important theoretical support and experimental basis for the development of new immune health products, meet the needs of the market and clinical patients, and enhance the physiological function and overall health of patients.

[0009] The present invention is achieved through the following technical solutions:

[0010] Application of a polygonatum and wolfberry water extract compound in the preparation of a product for improving immunological damage symptoms caused by chemotherapeutic drugs.

[0011] The mass ratio of the polygonatum water extract to the wolfberry water extract in the polygonatum and wolfberry water extract compound used for animal experiment verification is 1:1.

[0012] The mass ratio of the polygonatum water extract to the wolfberry water extract in the polygonatum water extract compound used for cell experiment verification is 1:100.

[0013] Application of a polygonatum and wolfberry water extract compound in preparing a product for enhancing the immune ability of immune cells.

[0014] Preferably, the immune cells include macrophages and T lymphocytes;

[0015] The enhancement of immune cell immunity is to enhance macrophage proliferation ability, enhance macrophage phagocytosis ability, enhance macrophage NO secretion ability, enhance T lymphocyte proliferation ability, inhibit macrophage apoptosis and inhibit T lymphocyte apoptosis.

[0016] A method for preparing a product for treating immunodeficiency caused by chemotherapy is disclosed. The product contains the compound water extract of polygonatum and wolfberry as an active ingredient.

[0017] Preferably, the polygonatum sibiricum and wolfberry water extract compound product includes any one or more of a polygonatum sibiricum and wolfberry water extract compound medicine and a polygonatum sibiricum and wolfberry water extract compound immune health product.

[0018] Preferably, the polygonatum sibiricum and wolfberry water extract compound product is used as a functional additive in the preparation of the polygonatum sibiricum and wolfberry water extract compound immune health care product.

[0019] Preferably, when preparing the polygonatum sibiricum and wolfberry water extract compound medicine, the polygonatum sibiricum and wolfberry water extract compound is used as the active ingredient and is prepared together with pharmaceutically acceptable excipients to obtain a pharmaceutically corresponding dosage form.

[0020] Compared with the prior art, the present invention has at least the following technical effects:

[0021] The present invention provides an application of a polygonatum and wolfberry water extract compound in the preparation of a product for improving immune damage symptoms caused by chemotherapy drugs. The polygonatum and wolfberry water extract compound can improve and enhance the macrophage and T cell function damage caused by chemotherapy drugs by inhibiting the apoptosis of immune cells, and can be added to immune active health products as a functional additive for use in auxiliary treatment and daily health care of chemotherapy patients.

[0022] Through research and exploration of scientific proportions, the compound of polygonatum and wolfberry water extract can inhibit the apoptosis of immune cells induced by chemotherapy drugs, provide a modern scientific basis for the traditional application of polygonatum and wolfberry, and provide important theoretical support and experimental basis for the development of new immune health products, meet the needs of the market and clinical patients, and enhance patients' physiological functions and overall health status.

[0023] Further verification and demonstration by combining animal experiments showed that compared with the single water extract of Polygonatum sibiricum or the water extract of Lycium barbarum, the complex of the two can more significantly enhance the immune function of immunodeficient mice, including more effectively inhibiting the apoptosis of immune cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of the maximum tolerated dose of Polygonatum sibiricum water extract and Lycium barbarum water extract on in vitro immune cells;

[0025] Figure 2 Schematic diagram of the effects of polygonatum, wolfberry and their complex on the proliferation ability of RAW264.7 macrophages;

[0026] Figure 3 Schematic diagram of the effects of polygonatum, wolfberry and their complex on the phagocytic ability of RAW264.7 macrophages;

[0027] Figure 4 Schematic diagram of the effects of polygonatum, wolfberry and their complex on the levels of normal cells and apoptotic cells in RAW264.7 macrophages;

[0028] Figure 5 Schematic diagram of the effect of polygonatum, wolfberry and their complex on the proliferation ability of Jurkat T lymphocytes;

[0029] Figure 6 Schematic diagram of the effects of polygonatum, wolfberry and their complex on the levels of normal cells and apoptotic cells in Jurkat T lymphocytes;

[0030] Figure 7 Schematic diagram of the effects of polygonatum, wolfberry and their complex on body weight, thymus mass and spleen mass of immunodeficient mice;

[0031] Figure 8 Schematic diagram of the effects of polygonatum, wolfberry and their complex on the proliferation of peritoneal macrophages in immunodeficient mice;

[0032] Fig. 9 Schematic diagram of the effects of polygonatum, wolfberry and their complex on the phagocytic ability of peritoneal macrophages in immunodeficient mice;

[0033] Fig.10 Schematic diagram of the effects of polygonatum, wolfberry and their complex on NO secretion capacity of peritoneal macrophages in immunodeficient mice;

[0034] Fig.11 Schematic diagram of the effect of polygonatum, wolfberry and their complex on the proliferation of spleen T lymphocytes in immunodeficient mice;

[0035] Fig.12 Schematic diagram of the effect of polygonatum, wolfberry and their complex on the number of spleen T lymphocytes in immunodeficient mice;

[0036] Fig.13 Schematic diagram of the effects of Polygonatum sibiricum, Lycium barbarum and their complex on normal thymus cells, early apoptotic cells and late apoptotic cells in immunodeficient mice. DETAILED DESCRIPTION

[0037] The embodiments of the present invention will be described in detail below in conjunction with examples. However, those skilled in the art will understand that the following examples are only used to illustrate the present invention and should not be construed as limiting the scope of the present invention. The specific conditions not specified in the examples are carried out according to conventional conditions or conditions recommended by the manufacturer. The products or instruments used without indicating the manufacturer are all conventional products that can be purchased commercially.

[0038] 1. Experimental design:

[0039] 1. Experimental Drugs

[0040] Vinorelbine tartrate was purchased from Shanghai MacLean Biochemical Technology Co., Ltd., product number: V820502; cyclophosphamide was purchased from Shanghai Yuanye Biotechnology Co., Ltd., product number: S30563; vitamin C was purchased from Shanghai Yuanye Biotechnology Co., Ltd., product number: S13001; polygonatum sibiricum water extract was purchased from Huangshan Hualuyuan Biotechnology Co., Ltd., product number: GSPS01; wolfberry water extract was purchased from Huangshan Hualuyuan Biotechnology Co., Ltd., product number: GSCW04.

[0041] 2. Experimental Methods

[0042] 2.1 Cell experiment: Efficacy of Polygonatum sibiricum-Wolfberry water extract complex in improving vinorelbine-induced immune cell damage in vitro and its evaluation method

[0043] 2.1.1 Screening of the maximum tolerated dose of Polygonatum sibiricum, Lycium barbarum and their combination in vitro

[0044] Experimental method: RAW264.7 cell line was cultured in DMEM high glucose medium, and Jurkat cell line was cultured in RPMI-1640 medium. Cells were seeded in 96-well plates at a concentration of 1×10 5 100 μL / well, cultured at 37°C, carbon dioxide (5%, V / V). After 24 hours, the cells were treated with the control group and different concentrations of polygonatum sibiricum water extract group and wolfberry water extract group. After 24 hours of co-culture, the supernatant was removed, washed with 100 μL PBS, and the cell survival rate was detected using a CCK-8 kit.

[0045] Concentration setting: The concentration gradient of Polygonatum sibiricum water extract was set to 0 (control group), 0.01, 0.05, 0.1, 0.5, 1.0 mg / mL, and the concentration gradient of Lycium barbarum water extract was set to 0 (control group), 0.5, 1, 10, 25, 50 mg / mL.

[0046] like Figure 1 Shown is a schematic diagram of the maximum tolerated dose screening of Polygonatum sibiricum water extract and Lycium barbarum water extract on in vitro immune cells.

[0047] like Figure 1 The experimental results show that the maximum tolerated dose of Polygonatum sibiricum water extract (PSE) in Figure A is 0.1 mg / mL, and the maximum tolerated dose of Lycium barbarum water extract (LFE) in Figure B is 10 mg / mL.

[0048] 2.1.2 Polygonatum sibiricum-wolfberry water extract complex improves RAW264.7 macrophage damage and apoptosis

[0049] Experimental method: RAW264.7 cell line was cultured in DMEM high glucose medium and vinorelbine was used as a low immunity inducer. The cells were seeded in a 96-well plate at a concentration of 1×10 5 The control group, model group, polygonatum water extract group (0.1 mg / mL), wolfberry water extract group (10 mg / mL) and complex group (polygonatum water extract concentration was 0.05 mg / mL, wolfberry water extract concentration was 5 mg / mL) were set up respectively. Except for the control group, the other groups were given vinorelbine and different drugs at the same time, and the macrophage proliferation ability, phagocytosis ability and apoptosis indexes were detected after 24 hours of co-culture.

[0050] Experimental results:

[0051] like Figure 2 Shown is a schematic diagram of the effects of polygonatum, wolfberry and their complex (Com) on the proliferation ability of RAW264.7 macrophages;

[0052] like Figure 3 Shown is a schematic diagram of the effects of polygonatum, wolfberry and their complex (Com) on the phagocytic ability of RAW264.7 macrophages;

[0053] like Figure 4 Shown is a schematic diagram of the effects of Polygonatum sibiricum, Lycium barbarum and their complex on the levels of normal cells (A) and apoptotic cells (B) in RAW264.7 macrophages.

[0054] (1) Combined with Figure 2 As shown, the results showed that the Polygonatum sibiricum-Wolfberry complex promoted the proliferation of macrophages, and its effect was significantly better than that of the single Polygonatum sibiricum water extract and Wolfberry water extract.

[0055] (2) Combined with Figure 3 As shown, the results showed that the Polygonatum sibiricum-Wolfberry complex enhanced the phagocytic function of macrophages, and the effect was significantly better than that of the single Polygonatum sibiricum water extract and Wolfberry water extract.

[0056] (3) Combined with Figure 4 As shown, the results showed that the Polygonatum sibiricum-Lycium barbarum complex inhibited macrophage apoptosis, and its effect was significantly better than that of the single Polygonatum sibiricum water extract and Lycium barbarum water extract.

[0057] 2.1.3 Polygonatum sibiricum-wolfberry water extract complex improves Jurkat T lymphocyte damage and apoptosis

[0058] Modeling method: Jurkat cell lines were cultured in RPMI-1640 medium, and vinorelbine was used as a low immunity inducer. The cells were inoculated in 96-well plates at a concentration of 1×10 5 The control group, model group, polygonatum water extract group (0.1 mg / mL), wolfberry water extract group (10 mg / mL) and complex group (polygonatum water extract concentration was 0.05 mg / mL, wolfberry water extract concentration was 5 mg / mL) were set up respectively. Except for the control group, the other groups were given vinorelbine and different drugs at the same time, and the T cell proliferation ability and apoptosis indexes were detected after 24 hours of co-culture.

[0059] Experimental results:

[0060] like Figure 5 Shown is a schematic diagram of the effects of polygonatum, wolfberry and their complex on the proliferation ability of Jurkat T lymphocytes;

[0061] like Figure 6Shown are the effects of Polygonatum sibiricum, Lycium barbarum and their complex on the levels of normal cells (A) and apoptotic cells (B) of Jurkat T lymphocytes.

[0062] (1) Combined with Figure 5 As shown, the results showed that the Polygonatum sibiricum-Wolfberry complex promoted the proliferation of T cells, and the effect was significantly better than that of the Polygonatum sibiricum water extract alone.

[0063] (2) Combined with Figure 6 As shown, the results showed that the Polygonatum sibiricum-Lycium barbarum complex inhibited T cell apoptosis, and its effect was significantly better than that of the single Polygonatum sibiricum water extract and Lycium barbarum water extract.

[0064] 2.2 Animal experiment: Efficacy of Polygonatum sibiricum-Wolfberry water extract complex in improving cyclophosphamide-induced immune damage in mice and its evaluation method

[0065] 2.2.1 Construction of mouse immune injury model and group intervention

[0066] Female SPF C57BL / 6J mice, 8 weeks old, were randomly divided into control group, model group, positive control group (vitamin C 15 mg / kg), polygonatum water extract group (300 mg / kg), wolfberry water extract group (300 mg / kg) and complex group (polygonatum water extract concentration of 150 mg / kg, wolfberry water extract concentration of 150 mg / kg), 5 mice in each group. Except for the normal control group, each group was given 80 mg / kg cyclophosphamide intraperitoneal injection, 200 μL / mouse, once a day, for 3 days. After the model was established, the model control group was gavaged with normal saline, and the positive control group and each intervention group were treated with drugs, 200 μL / mouse, once a day, and the gavage cycle was 18 days.

[0067] After the experiment, the mice were anesthetized with sodium pentobarbital and killed, tissue samples were collected for subsequent testing, and the data were analyzed and processed.

[0068] The experimental data were analyzed and plotted using GraphPad Prism software. The independent sample t test was used to compare the significant differences between the control group and the experimental group. The results were expressed as mean + standard error, and p < 0.05 indicated a significant difference.

[0069] Experimental results:

[0070] like Figure 7 Shown is a schematic diagram of the effects of Polygonatum sibiricum, Lycium barbarum and their complex on the body weight (A), thymus mass (B) and spleen mass (C) of immunodeficient mice;

[0071] like Figure 8 The figure shows the effect of polygonatum, wolfberry and their complex on the proliferation of peritoneal macrophages in immunodeficient mice;

[0072] like Fig. 9 The figure shows the effect of polygonatum, wolfberry and their complex on the phagocytic ability of peritoneal macrophages in immunodeficient mice;

[0073] like Fig.10 The figure shows the effect of polygonatum, wolfberry and their complex on NO secretion ability of peritoneal macrophages in immunodeficient mice;

[0074] like Fig.11 The figure shows the effect of polygonatum, wolfberry and their complex on the proliferation of spleen T lymphocytes in immunodeficient mice;

[0075] like Fig.12 The figure shows the effect of polygonatum, wolfberry and their complex on the number of spleen T lymphocytes in immunodeficient mice;

[0076] like Fig.13 Shown is a schematic diagram of the effects of Polygonatum sibiricum, Lycium barbarum and their complex on normal thymus cells (A), early apoptotic cells (B) and late apoptotic cells (C) in immunodeficient mice.

[0077] The results show:

[0078] (1) Combination Figure 7 The results showed that the Polygonatum-Wolfberry complex increased the body weight of immune-damaged mice and increased the immune organ index, and its effect was stronger than that of vitamin C.

[0079] (2) Combination Figure 8 The results showed that the Polygonatum sibiricum-Wolfberry complex enhanced the proliferation ability of peritoneal macrophages in immune-damaged mice, and its effect was significantly stronger than that of vitamin C.

[0080] (3) Combination Fig. 9 The results showed that the Polygonatum-Wolfberry complex enhanced the phagocytic ability of peritoneal macrophages in immunocompromised mice, and its effect was significantly stronger than that of vitamin C and the individual Polygonatum water extract and Wolfberry water extract.

[0081] (4) Combination Fig.10 The results showed that the Polygonatum-Wolfberry complex enhanced the NO secretion ability of peritoneal macrophages in immune-damaged mice, and its effect was significantly stronger than that of vitamin C and the single Polygonatum water extract and Wolfberry water extract.

[0082] (5) Combination Fig.11 The results showed that the Polygonatum-Wolfberry complex enhanced the proliferation ability of spleen T lymphocytes in immune-damaged mice, and its effect was significantly stronger than vitamin C and the individual Polygonatum water extract and Wolfberry water extract.

[0083] (6) Combination Fig.12The results showed that the Polygonatum sibiricum-Wolfberry complex increased the number of spleen T lymphocytes in immune-damaged mice.

[0084] (7) Combination Fig.13 The results showed that the Polygonatum sibiricum-Lycium barbarum complex inhibited thymocyte apoptosis in mice with immune damage.

[0085] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the protection scope of the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. Application of a compound of polygonatum and wolfberry water extract in the preparation of a product for improving immune damage symptoms caused by chemotherapeutic drugs.

2. Application of a compound of polygonatum and wolfberry water extract in the preparation of products for enhancing the immune ability of immune cells.

3. The use of a polygonatum and wolfberry water extract compound according to claim 2 in the preparation of a product for enhancing the immune ability of immune cells, characterized in that: The immune cells include macrophages and T lymphocytes; The enhancement of immune cell immunity is to enhance macrophage proliferation ability, enhance macrophage phagocytosis ability, enhance macrophage NO secretion ability, enhance T lymphocyte proliferation ability, inhibit macrophage apoptosis and inhibit T lymphocyte apoptosis.

4. Use of a compound product of polygonatum and wolfberry water extract in the preparation of a product for treating immunodeficiency caused by chemotherapy, characterized in that: The polygonatum sibiricum and wolfberry water extract compound product uses the polygonatum sibiricum and wolfberry water extract compound as an active ingredient.

5. The use of a composite product of polygonatum and wolfberry water extract according to claim 4 in the preparation of a product for treating immunodeficiency caused by chemotherapy, characterized in that: The polygonatum sibiricum and wolfberry water extract compound product includes any one or more of a polygonatum sibiricum and wolfberry water extract compound medicine and a polygonatum sibiricum and wolfberry water extract compound immune health care product.

6. Use of a composite product of polygonatum and wolfberry water extract according to claim 5 in the preparation of a product for treating immunodeficiency caused by chemotherapy, characterized in that: The polygonatum sibiricum and wolfberry water extract compound product is used as a functional additive in the preparation of the polygonatum sibiricum and wolfberry water extract compound immune health care product.

7. Use of a composite product of polygonatum and wolfberry water extract according to claim 6 in the preparation of a product for treating immunodeficiency caused by chemotherapy, characterized in that: When preparing the polygonatum sibiricum and wolfberry water extract compound medicine, the polygonatum sibiricum and wolfberry water extract compound is used as the active ingredient and is prepared together with pharmaceutically acceptable excipients to obtain a pharmaceutically corresponding dosage form.

Images