Application of royal jelly protein in medicine for treating cardiovascular diseases

Abstract

The invention discloses an application of royal jelly protein in a medicine for treating cardiovascular diseases, which comprises the following steps: S1, extracting royal jelly protein: extracting royal jelly protein from royal jelly, and preparing a royal jelly protein solution; s2, preparing a ligusticum wallichii and pseudo-ginseng alcohol extract: taking ligusticum wallichii, pseudo-ginseng and dalbergia wood oil as raw materials, mixing, filtering and concentrating to prepare the ligusticum wallichii and pseudo-ginseng alcohol extract; s3, preparing a mixed extracting solution: taking rhodiola rosea, nacre mother of pearl and honey-fried licorice root as raw materials, adding water to decoct, combining the extracting solution, filtering, and concentrating the filtrate to obtain the mixed extracting solution; s4, mixing; and S5, concentrating. The royal jelly protein is added into the raw materials of the medicine, and microelements, amino acid and mineral nutrient substances contained in the royal jelly protein can nourish myocardium and improve the metabolic function of the myocardium, so that the function of the heart can be enhanced, and the heart is helped to better convey blood to the whole body.

Description

Technical Field

[0001] The present invention belongs to the field of pharmaceutical technology, and specifically relates to the application of royal jelly protein in drugs for cardiovascular diseases. Background Art

[0002] Cardiovascular disease, also known as circulatory system disease, is a series of diseases involving the circulatory system. The circulatory system refers to the organs and tissues that transport blood in the human body, mainly including the heart, blood vessels (arteries, veins, capillaries), which can be subdivided into acute and chronic, and are generally related to arteriosclerosis. These diseases have similar causes, onset processes and treatment methods. In recent years, the incidence and mortality of cardiovascular and cerebrovascular diseases have been on the rise, both in rural and urban areas. Among them, the mortality rate of cardiovascular diseases such as coronary heart disease, angina pectoris, myocardial ischemia combined with arrhythmia ranks first among various diseases, seriously threatening human life and health. At present, the drugs used in clinical treatment of cardiovascular diseases are mainly chemical drugs and traditional Chinese medicine preparations.

[0003] Royal jelly protein is a protein extracted from royal jelly. The use of royal jelly can enhance the body's resistance, promote the regeneration and repair of internal organs and tissues, adjust endocrine and metabolism, and effectively increase appetite, improve sleep and promote growth and development. It has extremely strong health care functions and medical effects on the human body.

[0004] Recent studies have found that using royal jelly protein in drugs for the treatment of cardiovascular diseases can nourish the myocardium, improve the metabolic function of the myocardium, thereby enhancing the function of the heart. It can also regulate blood circulation, improve the body's resistance, and improve physical condition. It has a good auxiliary effect in preventing hyperlipidemia and atherosclerosis. Therefore, it is necessary to study the application of royal jelly protein in drugs for the treatment of cardiovascular diseases. Summary of the invention

[0005] The purpose of the present invention is to provide the use of royal jelly protein in drugs for cardiovascular diseases to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solution: Application of royal jelly protein in drugs for cardiovascular diseases, comprising the following steps:

[0007] S1. Extracting royal jelly protein: extracting royal jelly protein from royal jelly and preparing a royal jelly protein solution;

[0008] S2. Preparation of Ligusticum chuanxiong Panax notoginseng alcohol extract: Ligusticum chuanxiong, Panax notoginseng, and dalbergia odorifera oil were used as raw materials, and the mixture was mixed, filtered, and concentrated to obtain Ligusticum chuanxiong Panax notoginseng alcohol extract;

[0009] S3. Preparation of a mixed extract: Using rhodiola rosea, mother of pearl, licorice as raw materials, boiling with water, combining the extracts and filtering, and concentrating the filtrate to obtain a mixed extract;

[0010] S4 mixing: the prepared royal jelly protein solution, Chuanxiong Panax notoginseng alcohol extract and the mixed extract were mixed and stirred to obtain a mixed solution;

[0011] S5. Concentration: The mixed solution is heated and concentrated, and stirred while heating until it becomes a paste, and then granulated to obtain the finished drug product.

[0012] Preferably, the specific method for extracting royal jelly protein in S1 comprises the following steps:

[0013] S101. Weigh royal jelly, mix with water at 3-5°C, adjust pH with NaOH, adjust ionic strength with NaCl, and remove particles with a 0.12-0.24 μm microporous membrane;

[0014] S102. Extract the royal jelly protein solution, use ultrafiltration membrane M1 with a molecular weight cutoff of 90-110kDa to separate and remove large molecular impurities, and then use ultrafiltration membrane M2 with a molecular weight cutoff of 42-58kDa to separate and concentrate the royal jelly protein in the M1 permeate. When concentrated to 1 / 3 of the volume, add pure water and allow the small molecule solution to pass through M2. The resulting permeate is a purified and concentrated royal jelly protein solution.

[0015] Preferably, the specific steps of preparing the alcohol extract of Ligusticum chuanxiong and Panax notoginseng in S2 are:

[0016] S201. Take raw materials: Take raw materials in the following parts by weight: 20-40 parts of Chuanxiong, 6-12 parts of Panax notoginseng, 0.3-0.6 parts of dalbergia odorifera oil;

[0017] S202. Crushing: crushing and mixing Chuanxiong and Panax notoginseng into an extraction tank;

[0018] S203. Boil: Add 5 times the amount of water to the extraction tank, boil for 2 hours, filter, extract the residue for the second time, add 4 times the amount of water, boil for 1 hour, filter, extract the residue for the third time, add 2 times the amount of water, boil for 0.5 hours, filter, discard the residue, and combine the filtrate obtained three times;

[0019] S204. Filtration: Ultrafiltration is performed on the obtained filtrate to obtain an ultrafiltrate:

[0020] S205. Concentration: concentrating the ultrafiltrate to obtain a concentrated solution;

[0021] S206. Prepare the alcohol extract of Ligusticum chuanxiong and Panax notoginseng: spray the agarwood oil and mix well to obtain the alcohol extract of Ligusticum chuanxiong and Panax notoginseng.

[0022] Preferably, during the filtration in S204, the filtrate is ultrafiltered with an ultrafiltration membrane, the filtration method adopts cross-flow filtration, the molecular weight cutoff of the ultrafiltration membrane is 6000-20000, and the ultrafiltration membrane is selected from one of diacetyl cellulose membrane, triacetyl cellulose membrane, cyanoethyl cellulose acetate membrane, and polysulfone membrane.

[0023] Preferably, the filtration in S204 also includes intermittently introducing an inert gas, and the ventilation method is to ventilate once every 0.5-1.5 hours, and each time for 1 minute.

[0024] Preferably, the method for preparing the mixed extract in S3 is specifically:

[0025] S301. Take the following raw materials in parts by weight: 12-18 parts of Rhodiola rosea, 24-36 parts of mother of pearl and 2-8 parts of licorice;

[0026] S302. The above raw materials were mixed, 3-5 times water was added and boiled 1-3 times for 30-60min, and the extracts were combined;

[0027] S303. Filter the combined extracts, heat and concentrate the filtrate to obtain a mixed extract.

[0028] Preferably, before taking the raw materials in S301, the step of removing impurities, washing and crushing the Rhodiola rosea, mother-of-pearl and licorice raw materials is also included.

[0029] Preferably, the stirring speed of S4 is 200-400 r / min, and the stirring time is 30-60 min.

[0030] Preferably, the dosage form of the drug is capsule, tablet, granule and pill.

[0031] Preferably, the drug further comprises any one or more pharmaceutical excipients selected from the group consisting of starch, dextrin, lactose, microcrystalline cellulose, hydroxypropyl methylcellulose, polyethylene glycol, magnesium stearate, micropowdered silica gel, xylitol, lactitol, glucose, glycine, and mannitol.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] (1) The present invention adds royal jelly protein to the raw materials of the medicine, and utilizes the trace elements, amino acids and mineral nutrients contained in the royal jelly protein to nourish the myocardium and improve the metabolic function of the myocardium, thereby enhancing the function of the heart and helping the heart to better transport blood to the whole body;

[0034] (2) The present invention applies royal jelly protein to drugs for cardiovascular diseases. The minerals in royal jelly protein can also promote the growth of hemoglobin, increase the elasticity of blood vessels, and promote blood circulation. The potassium element can also remove the attachments on the inner wall of blood vessels, and has a good nourishing effect on cardiovascular and cerebrovascular vessels. People with poor cardiovascular disease can regulate blood circulation and improve their body resistance by consuming royal jelly protein for a long time. It has a good auxiliary effect on preventing hyperlipidemia and atherosclerosis.

[0035] (3) The present invention adds the alcohol extract of Ligusticum chuanxiong and Panax notoginseng to the medicine, which can invigorate blood circulation and remove blood stasis, and has a certain anti-angina effect. In addition, by adding the mixed extract to the medicine, Rhodiola rosea can invigorate qi and invigorate blood circulation, nourish yin and strengthen the body, pearl mother can calm the liver and suppress yang, calm the nerves and improve eyesight, and calm the mind, and roasted liquorice can invigorate the spleen and harmonize the stomach, invigorate qi and restore the pulse, thereby facilitating the prevention and control of cardiovascular diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 is a flow chart of the present invention;

[0037] Figure 2 This is a flow chart of extracting royal jelly protein according to the present invention;

[0038] Figure 3 The present invention is a flow chart for preparing the alcohol extract of Ligusticum chuanxiong and Panax notoginseng. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] Example 1

[0041] See also Figure 1-Figure 3 The present invention provides a technical solution: application of royal jelly protein in drugs for cardiovascular diseases, comprising the following steps:

[0042] S1. Extracting royal jelly protein: extracting royal jelly protein from royal jelly and preparing a royal jelly protein solution;

[0043] S2. Preparation of Ligusticum chuanxiong Panax notoginseng alcohol extract: Ligusticum chuanxiong, Panax notoginseng, and dalbergia odorifera oil were used as raw materials, and the mixture was mixed, filtered, and concentrated to obtain Ligusticum chuanxiong Panax notoginseng alcohol extract;

[0044] S3. Preparation of a mixed extract: Using rhodiola rosea, mother of pearl, licorice as raw materials, boiling with water, combining the extracts and filtering, and concentrating the filtrate to obtain a mixed extract;

[0045] S4 mixing: the prepared royal jelly protein solution, Chuanxiong Panax notoginseng alcohol extract and the mixed extract were mixed and stirred to obtain a mixed solution;

[0046] S5. Concentration: The mixed solution is heated and concentrated, and stirred while heating until it becomes a paste, and then granulated to obtain the finished drug product.

[0047] In this embodiment, preferably, the specific method for extracting royal jelly protein in S1 comprises the following steps:

[0048] S101. Weigh royal jelly, mix with water at 3°C, adjust pH with NaOH, adjust ionic strength with NaCl, and remove particles with a 0.12 μm microporous membrane;

[0049] S102. Extract the royal jelly protein solution, use ultrafiltration membrane M1 with a molecular weight cutoff of 90kDa to separate and remove large molecular impurities, and then use ultrafiltration membrane M2 with a molecular weight cutoff of 42kDa to separate and concentrate the royal jelly protein in the M1 permeate. When concentrated to 1 / 3 of the volume, add pure water and allow the small molecule solution to pass through M2. The resulting permeate is a purified and concentrated royal jelly protein solution.

[0050] In this embodiment, preferably, the specific steps of preparing the alcohol extract of Ligusticum chuanxiong and Panax notoginseng in S2 are:

[0051] S201. Take raw materials: Take raw materials according to the following weight parts: 20 parts of Chuanxiong, 6 parts of Panax notoginseng, 0.3 parts of dalbergia odorifera oil;

[0052] S202. Crushing: crushing and mixing Chuanxiong and Panax notoginseng into an extraction tank;

[0053] S203. Boil: Add 5 times the amount of water to the extraction tank, boil for 2 hours, filter, extract the residue for the second time, add 4 times the amount of water, boil for 1 hour, filter, extract the residue for the third time, add 2 times the amount of water, boil for 0.5 hours, filter, discard the residue, and combine the filtrate obtained three times;

[0054] S204. Filtration: Ultrafiltration is performed on the obtained filtrate to obtain an ultrafiltrate:

[0055] S205. Concentration: concentrating the ultrafiltrate to obtain a concentrated solution;

[0056] S206. Prepare the alcohol extract of Ligusticum chuanxiong and Panax notoginseng: spray the agarwood oil and mix well to obtain the alcohol extract of Ligusticum chuanxiong and Panax notoginseng.

[0057] In this embodiment, preferably, during the filtration in S204, the filtrate is ultrafiltered using an ultrafiltration membrane, the filtration method is cross-flow filtration, the molecular weight cutoff of the ultrafiltration membrane is 6000, and the ultrafiltration membrane is selected from cellulose diacetate membrane.

[0058] In this embodiment, preferably, the filtration in S204 also includes intermittently introducing inert gas, and the ventilation method is ventilation once every 0.5 hours, and each time for 1 minute.

[0059] In this embodiment, preferably, the method for preparing the mixed extract in S3 is specifically:

[0060] S301. Take the following raw materials in parts by weight: 12 parts of Rhodiola rosea, 24 parts of mother-of-pearl and 2 parts of licorice;

[0061] S302. The above raw materials were mixed, decocted once with 3 times water for 30 min, and the extracts were combined;

[0062] S303. Filter the combined extracts, heat and concentrate the filtrate to obtain a mixed extract.

[0063] In this embodiment, preferably, before taking the raw materials in S301, the step of removing impurities, washing and crushing the Rhodiola rosea, mother of pearl and licorice raw materials is also included.

[0064] In this embodiment, preferably, the stirring speed of S4 is 200 r / min and the stirring time is 30 min.

[0065] In this embodiment, preferably, the dosage form of the drug is a capsule.

[0066] In this embodiment, preferably, the medicine further comprises starch, dextrin and lactose.

[0067] Example 2

[0068] See also Figure 1-Figure 3 The present invention provides a technical solution: application of royal jelly protein in drugs for cardiovascular diseases, comprising the following steps:

[0069] S1. Extracting royal jelly protein: extracting royal jelly protein from royal jelly and preparing a royal jelly protein solution;

[0070] S2. Preparation of Ligusticum chuanxiong Panax notoginseng alcohol extract: Ligusticum chuanxiong, Panax notoginseng, and dalbergia odorifera oil were used as raw materials, and the mixture was mixed, filtered, and concentrated to obtain Ligusticum chuanxiong Panax notoginseng alcohol extract;

[0071] S3. Preparation of a mixed extract: Using rhodiola rosea, mother of pearl, licorice as raw materials, boiling with water, combining the extracts and filtering, and concentrating the filtrate to obtain a mixed extract;

[0072] S4 mixing: the prepared royal jelly protein solution, Chuanxiong Panax notoginseng alcohol extract and the mixed extract were mixed and stirred to obtain a mixed solution;

[0073] S5. Concentration: The mixed solution is heated and concentrated, and stirred while heating until it becomes a paste, and then granulated to obtain the finished drug product.

[0074] In this embodiment, preferably, the specific method for extracting royal jelly protein in S1 comprises the following steps:

[0075] S101. Weigh royal jelly, mix with water at 5°C, adjust pH with NaOH, adjust ionic strength with NaCl, and remove particles with a 0.24 μm microporous membrane;

[0076] S102. Extract the royal jelly protein solution, use ultrafiltration membrane M1 with a molecular weight cutoff of 110kDa to separate and remove large molecular impurities, and then use ultrafiltration membrane M2 with a molecular weight cutoff of 58kDa to separate and concentrate the royal jelly protein in the M1 permeate. When concentrated to 1 / 3 of the volume, add pure water and allow the small molecule solution to pass through M2. The resulting permeate is a purified and concentrated royal jelly protein solution.

[0077] In this embodiment, preferably, the specific steps of preparing the alcohol extract of Ligusticum chuanxiong and Panax notoginseng in S2 are:

[0078] S201. Take raw materials: Take raw materials according to the following weight parts: 40 parts of Chuanxiong, 12 parts of Panax notoginseng, 0.6 parts of dalbergia odorifera oil;

[0079] S202. Crushing: crushing and mixing Chuanxiong and Panax notoginseng into an extraction tank;

[0080] S203. Boil: Add 5 times the amount of water to the extraction tank, boil for 2 hours, filter, extract the residue for the second time, add 4 times the amount of water, boil for 1 hour, filter, extract the residue for the third time, add 2 times the amount of water, boil for 0.5 hours, filter, discard the residue, and combine the filtrate obtained three times;

[0081] S204. Filtration: Ultrafiltration is performed on the obtained filtrate to obtain an ultrafiltrate:

[0082] S205. Concentration: concentrating the ultrafiltrate to obtain a concentrated solution;

[0083] S206. Prepare the alcohol extract of Ligusticum chuanxiong and Panax notoginseng: spray the agarwood oil and mix well to obtain the alcohol extract of Ligusticum chuanxiong and Panax notoginseng.

[0084] In this embodiment, preferably, during the filtration in S204, the filtrate is ultrafiltered using an ultrafiltration membrane, the filtration method adopts cross-flow filtration, the molecular weight cutoff of the ultrafiltration membrane is 20,000, and the ultrafiltration membrane is selected from cellulose triacetate membrane.

[0085] In this embodiment, preferably, the filtration in S204 also includes intermittently introducing inert gas, and the ventilation method is ventilation once every 1.5 hours, each time for 1 minute.

[0086] In this embodiment, preferably, the method for preparing the mixed extract in S3 is specifically:

[0087] S301. Take the following raw materials in parts by weight: 18 parts of Rhodiola rosea, 36 parts of mother of pearl and 8 parts of licorice;

[0088] S302. The above raw materials were mixed, decocted 3 times with 5 times water, the decoction time was 60min, and the extracts were combined;

[0089] S303. Filter the combined extracts, heat and concentrate the filtrate to obtain a mixed extract.

[0090] In this embodiment, preferably, before taking the raw materials in S301, the step of removing impurities, washing and crushing the Rhodiola rosea, mother of pearl and licorice raw materials is also included.

[0091] In this embodiment, preferably, the rotation speed of the stirring in S4 is 400 r / min and the stirring time is 60 min.

[0092] In this embodiment, preferably, the dosage form of the drug is granules.

[0093] In this embodiment, preferably, the medicine further comprises starch, lactose, lactitol and glucose.

[0094] Example 3

[0095] See also Figure 1-Figure 3 The present invention provides a technical solution: application of royal jelly protein in drugs for cardiovascular diseases, comprising the following steps:

[0096] S1. Extracting royal jelly protein: extracting royal jelly protein from royal jelly and preparing a royal jelly protein solution;

[0097] S2. Preparation of Ligusticum chuanxiong Panax notoginseng alcohol extract: Ligusticum chuanxiong, Panax notoginseng, and dalbergia odorifera oil were used as raw materials, and the mixture was mixed, filtered, and concentrated to obtain Ligusticum chuanxiong Panax notoginseng alcohol extract;

[0098] S3. Preparation of a mixed extract: Using rhodiola rosea, mother of pearl, licorice as raw materials, boiling with water, combining the extracts and filtering, and concentrating the filtrate to obtain a mixed extract;

[0099] S4 mixing: the prepared royal jelly protein solution, Chuanxiong Panax notoginseng alcohol extract and the mixed extract were mixed and stirred to obtain a mixed solution;

[0100] S5. Concentration: The mixed solution is heated and concentrated, and stirred while heating until it becomes a paste, and then granulated to obtain the finished drug product.

[0101] In this embodiment, preferably, the specific method for extracting royal jelly protein in S1 comprises the following steps:

[0102] S101. Weigh royal jelly, mix with water at 4°C, adjust pH with NaOH, adjust ionic strength with NaCl, and remove particles with a 0.2 μm microporous membrane;

[0103] S102. Extract the royal jelly protein solution, use ultrafiltration membrane M1 with a molecular weight cutoff of 100kDa to separate and remove large molecular impurities, and then use ultrafiltration membrane M2 with a molecular weight cutoff of 48kDa to separate and concentrate the royal jelly protein in the M1 permeate. When concentrated to 1 / 3 of the volume, add pure water and allow the small molecule solution to pass through M2. The resulting permeate is a purified and concentrated royal jelly protein solution.

[0104] In this embodiment, preferably, the specific steps of preparing the alcohol extract of Ligusticum chuanxiong and Panax notoginseng in S2 are:

[0105] S201. Take raw materials: Take raw materials according to the following weight parts: 30 parts of Chuanxiong, 8 parts of Panax notoginseng, 0.4 parts of dalbergia odorifera oil;

[0106] S202. Crushing: crushing and mixing Chuanxiong and Panax notoginseng into an extraction tank;

[0107] S203. Boil: Add 5 times the amount of water to the extraction tank, boil for 2 hours, filter, extract the residue for the second time, add 4 times the amount of water, boil for 1 hour, filter, extract the residue for the third time, add 2 times the amount of water, boil for 0.5 hours, filter, discard the residue, and combine the filtrate obtained three times;

[0108] S204. Filtration: Ultrafiltration is performed on the obtained filtrate to obtain an ultrafiltrate:

[0109] S205. Concentration: concentrating the ultrafiltrate to obtain a concentrated solution;

[0110] S206. Prepare the alcohol extract of Ligusticum chuanxiong and Panax notoginseng: spray the agarwood oil and mix well to obtain the alcohol extract of Ligusticum chuanxiong and Panax notoginseng.

[0111] In this embodiment, preferably, during the filtration in S204, the filtrate is ultrafiltered using an ultrafiltration membrane, the filtration method adopts cross-flow filtration, the molecular weight cutoff of the ultrafiltration membrane is 10,000, and the ultrafiltration membrane is selected from cyanoethyl cellulose acetate membrane.

[0112] In this embodiment, preferably, the filtration in S204 also includes intermittently introducing inert gas, and the ventilation method is ventilation once every 1 hour, each time for 1 minute.

[0113] In this embodiment, preferably, the method for preparing the mixed extract in S3 is specifically:

[0114] S301. Take the following raw materials in parts by weight: 16 parts of Rhodiola rosea, 28 parts of mother-of-pearl and 6 parts of licorice;

[0115] S302. The above raw materials were mixed, decocted twice with 4 times of water for 40 min, and the extracts were combined;

[0116] S303. Filter the combined extracts, heat and concentrate the filtrate to obtain a mixed extract.

[0117] In this embodiment, preferably, before taking the raw materials in S301, the step of removing impurities, washing and crushing the Rhodiola rosea, mother of pearl and licorice raw materials is also included.

[0118] In this embodiment, preferably, the stirring speed of S4 is 300 r / min and the stirring time is 40 min.

[0119] In this embodiment, preferably, the dosage form of the drug is pills.

[0120] In this embodiment, preferably, the medicine further comprises lactose, xylitol, lactitol, glucose, glycine and mannitol.

[0121] Principles and advantages of the present invention:

[0122] The present invention adds royal jelly protein to the raw materials of the medicine, and utilizes the trace elements, amino acids and mineral nutrients contained in the royal jelly protein to nourish the myocardium and improve the metabolic function of the myocardium, thereby enhancing the function of the heart and helping the heart to better transport blood to the whole body;

[0123] The present invention applies royal jelly protein to the medicine for cardiovascular diseases. The minerals in royal jelly protein can also promote the growth of hemoglobin, increase the elasticity of blood vessels, and promote blood circulation. The potassium element can also remove the attachments on the inner wall of blood vessels, and has a good nourishing effect on cardiovascular and cerebrovascular vessels. People with poor cardiovascular disease can regulate blood circulation and improve their body resistance by taking royal jelly protein for a long time. It has a good auxiliary effect on preventing hyperlipidemia and atherosclerosis.

[0124] The present invention adds chuanxiong and panax notoginseng alcohol extract to the medicine, and the chuanxiong and panax notoginseng can promote blood circulation and remove blood stasis, and the chuanxiong and panax notoginseng has a certain anti-angina effect. In addition, by adding a mixed extract to the medicine, rhodiola rosea can invigorate qi and invigorate blood circulation, nourish yin and strengthen the body, pearl mother can calm the liver and suppress yang, calm shock and improve eyesight, and calm the nerves, and roasted liquorice can invigorate the spleen and stomach, invigorate qi and restore the pulse, thereby being beneficial to the prevention and control of cardiovascular diseases.

[0125] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. The use of royal jelly protein in drugs for cardiovascular diseases, characterized in that: The following steps are involved: S1. Extracting royal jelly protein: extracting royal jelly protein from royal jelly and preparing a royal jelly protein solution; S2. Preparation of Ligusticum chuanxiong Panax notoginseng alcohol extract: Ligusticum chuanxiong, Panax notoginseng, and dalbergia odorifera oil were used as raw materials, and the mixture was mixed, filtered, and concentrated to obtain Ligusticum chuanxiong Panax notoginseng alcohol extract; S3. Preparation of a mixed extract: Using rhodiola rosea, mother of pearl, licorice as raw materials, boiling with water, combining the extracts and filtering, and concentrating the filtrate to obtain a mixed extract; S4 mixing: the prepared royal jelly protein solution, Chuanxiong Panax notoginseng alcohol extract and the mixed extract were mixed and stirred to obtain a mixed solution; S5. Concentration: The mixed solution is heated and concentrated, and stirred while heating until it becomes a paste, and then granulated to obtain the finished drug product.

2. The use of royal jelly protein in a drug for cardiovascular disease according to claim 1, characterized in that: The specific method for extracting royal jelly protein in S1 comprises the following steps: S101. Weigh royal jelly, mix with water at 3-5°C, adjust pH with NaOH, adjust ionic strength with NaCl, and remove particles with a 0.12-0.24 μm microporous membrane; S102. Extract the royal jelly protein solution, use ultrafiltration membrane M1 with a molecular weight cutoff of 90-110kDa to separate and remove large molecular impurities, and then use ultrafiltration membrane M2 with a molecular weight cutoff of 42-58kDa to separate and concentrate the royal jelly protein in the M1 permeate. When concentrated to 1 / 3 of the volume, add pure water and allow the small molecule solution to pass through M2. The resulting permeate is a purified and concentrated royal jelly protein solution.

3. The use of royal jelly protein in a drug for cardiovascular disease according to claim 1, characterized in that: The specific steps of preparing the alcohol extract of Ligusticum chuanxiong and Panax notoginseng in S2 are: S201. Take raw materials: Take raw materials according to the following weight parts: 20-40 parts of Chuanxiong, 6-12 parts of Panax notoginseng, 0.3-0.6 parts of dalbergia odorifera oil; S202. Crushing: crushing and mixing Chuanxiong and Panax notoginseng into an extraction tank; S203. Boil: Add 5 times the amount of water to the extraction tank, boil for 2 hours, filter, extract the residue for the second time, add 4 times the amount of water, boil for 1 hour, filter, extract the residue for the third time, add 2 times the amount of water, boil for 0.5 hours, filter, discard the residue, and combine the filtrate obtained three times; S204. Filtration: Ultrafiltration is performed on the obtained filtrate to obtain an ultrafiltrate: S205. Concentration: concentrating the ultrafiltrate to obtain a concentrate; S206. Prepare the alcohol extract of Ligusticum chuanxiong and Panax notoginseng: spray the agarwood oil and mix well to obtain the alcohol extract of Ligusticum chuanxiong and Panax notoginseng.

4. The use of royal jelly protein in a drug for cardiovascular disease according to claim 3, characterized in that: During the filtration in S204, the filtrate is ultrafiltered using an ultrafiltration membrane, and the filtering method adopts cross-flow filtration. The molecular weight cutoff of the ultrafiltration membrane is 6000-20000, and the ultrafiltration membrane is selected from one of diacetyl cellulose membrane, triacetyl cellulose membrane, cyanoethyl cellulose acetate membrane, and polysulfone membrane.

5. The use of royal jelly protein in a drug for cardiovascular disease according to claim 4, characterized in that: The filtration in S204 also includes intermittently introducing inert gas, and the ventilation method is ventilation once every 0.5-1.5 hours, and each time for 1 minute.

6. The use of royal jelly protein in a drug for cardiovascular disease according to claim 1, characterized in that: The method for preparing the mixed extract in S3 is specifically as follows: S301. Take the following raw materials in parts by weight: 12-18 parts of Rhodiola rosea, 24-36 parts of mother of pearl and 2-8 parts of licorice; S302. The above raw materials were mixed, 3-5 times water was added and boiled 1-3 times for 30-60min, and the extracts were combined; S303. Filter the combined extracts, heat and concentrate the filtrate to obtain a mixed extract.

7. The use of royal jelly protein in a drug for cardiovascular disease according to claim 6, characterized in that: The step S301 also includes removing impurities, washing and crushing the Rhodiola rosea, mother-of-pearl and licorice raw materials before taking the raw materials.

8. The use of royal jelly protein in a drug for cardiovascular disease according to claim 1, characterized in that: The rotation speed of S4 during stirring is 200-400 r / min, and the stirring time is 30-60 min.

9. The use of royal jelly protein in a drug for cardiovascular disease according to claim 1, characterized in that: The dosage form of the drug is capsule, tablet, granule and pill.

10. The use of royal jelly protein in a drug for cardiovascular disease according to claim 9, characterized in that: The medicine also contains any one or more pharmaceutical excipients of starch, dextrin, lactose, microcrystalline cellulose, hydroxypropyl methylcellulose, polyethylene glycol, magnesium stearate, micropowder silica gel, xylitol, lactitol, glucose, glycine and mannitol.

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