Pressurizing and draining device applied to DNA (Deoxyribose Nucleic Acid) synthesizer

Abstract

The invention relates to the field of biotechnology equipment, in particular to a pressurizing and liquid discharging device applied to a DNA synthesizer, the device comprises a synthesis plate, a gas protection assembly, a pressurizing device and a waste liquid tank, a reaction tank on the synthesis plate is connected with the gas protection assembly through a liquid discharging needle hole, the pressurizing device can increase the air pressure in the reaction tank, and the waste liquid tank is connected with the gas protection assembly through a liquid discharging needle hole. The liquid can overcome surface tension and lower section air pressure to be discharged into the waste liquid tank; the pressurizing and liquid discharging device applied to the DNA synthesizer is simple in structure, low in manufacturing cost and convenient to operate, the sealing effect can be achieved through surface tension of liquid and pressure provided by an air column, and liquid discharging of a plurality of reaction tanks can be controlled at the same time only by providing small air pressure.

Description

Technical Field

[0001] The present application relates to the field of biotechnology equipment, and in particular to a pressurized liquid discharge device used in a DNA synthesizer. Background Art

[0002] In a DNA synthesizer, the drainage process is one of the key links to ensure the smooth progress of the reaction. With the rapid development of biotechnology, high-throughput DNA synthesis equipment has become more and more popular, which has put forward higher requirements on the efficiency and reliability of the drainage system.

[0003] At present, the common drainage method is to install a solenoid valve on each drainage channel, and discharge the liquid by precisely controlling the opening of each solenoid valve under the action of its own gravity; although this traditional method can achieve precise control, it still has many shortcomings in practical applications. When there are many workstation holes, a large number of solenoid valves are required, which leads to a significant increase in overall cost, and the maintenance is complicated. The coordinated control of the multi-solenoid valve system is also difficult. Therefore, there is an urgent need for a new type of drainage device that can reduce costs while improving reliability and adaptability. Summary of the invention

[0004] In order to overcome the shortcomings of the above-mentioned prior art, the present application provides a pressurized liquid discharge device applied to a DNA synthesizer, which has a simple structure, low manufacturing cost, and easy operation. It can utilize the surface tension of the liquid itself and the pressure provided by the air column to achieve a sealing effect, and only needs to provide a small air pressure to control the discharge of multiple reaction tanks at the same time.

[0005] This application is implemented through the following technical solutions: A pressurized liquid discharge device applied to a DNA synthesizer comprises a synthesis plate, an air retaining component, a pressurizing device and a waste liquid tank. The synthesis plate is evenly provided with a plurality of reaction tanks for synthesizing DNA, the reaction tanks are arranged in a vertical direction, and a liquid discharge pinhole is provided at the bottom of the reaction tank. The liquid in the reaction tank is retained in the reaction tank at the input end of the liquid discharge pinhole by the support of its own surface tension and the air pressure of the lower section of the air; the air retaining component is connected to the output end of the liquid discharge pinhole through a pipeline, and is used to seal the air in the lower section of the liquid discharge pinhole by utilizing the surface tension of the liquid, so as to provide supporting air pressure for the liquid tension interface at the input end of the liquid discharge pinhole; the pressurizing device is used to increase the air pressure in the reaction tank, drive the liquid in the reaction tank to overcome its own surface tension and the supporting force of the air pressure of the lower section of the air, and discharge from the liquid discharge pinhole; the waste liquid tank is used to water-seal the output end of the air retaining component and collect the liquid discharged from the reaction tank.

[0006] By adopting the above technical scheme, the problems existing in the prior art can be effectively solved. Specifically, a drainage pinhole is provided at the bottom of the reaction tank, so that the liquid can be retained in the reaction tank at the input end of the drainage pinhole under the support of its own surface tension and the air pressure of the lower section, thereby avoiding the cost increase problem of using a large number of solenoid valves; the air retention component is connected to the output end of the drainage pinhole through a pipeline, and the air in the lower section of the drainage pinhole is sealed by the surface tension of the liquid, and the supporting air pressure is provided for the liquid tension interface at the input end of the drainage pinhole to ensure that the liquid does not flow out at will, thereby improving the stability and reliability of the system, and the waste liquid tank can be used to water seal the air retention component to prevent the air in the air retention component from leaking out; when draining, it is only necessary to provide a small air pressure to the reaction tank to complete it. Compared with the traditional elastic one-way valve blocking the flow channel, its sealing mechanism can better ensure the safety and reliability of the device. On the one hand, because the DNA reaction The waste liquid has acidity, alkalinity or other salt components, which can easily corrode traditional metal elastic parts and affect their lifespan. If elastic parts made of special materials are used, it will inevitably lead to an increase in production costs. On the other hand, traditional elastic parts require a large impact air pressure to overcome the elastic force and open the blocked channel, and their rebound speed is fast. It is necessary to continuously pass high air pressure for a long time to discharge the waste liquid, and the power consumption is large; the pressurizing device is used to increase the air pressure in the reaction tank, driving the liquid in the reaction tank to overcome its own surface tension and the supporting force of the lower air pressure, and be discharged from the drainage pinhole, realizing an efficient and controllable waste liquid discharge process; the waste liquid tank is used to collect the liquid discharged from the reaction tank, further simplifying the system structure, reducing the complexity of the equipment, and reducing the maintenance cost. In summary, this technical solution not only solves the problems of high cost and operation complexity in the prior art, but also improves the working efficiency and stability of the synthesizer.

[0007] Optionally, the bottom of the reaction tank is in an arc structure.

[0008] By adopting the above technical solution, the arc structure of the bottom of the reaction tank can effectively reduce the amount of liquid residue and improve the drainage efficiency. At the same time, the design of the arc structure helps to increase the curvature at the input end of the drainage pinhole, increase the surface tension of the liquid itself at the input end of the drainage pinhole, and improve the drainage pinhole's carrying capacity for the liquid.

[0009] Optionally, the diameter of the drainage pinhole is 0.5~0.3mm.

[0010] By adopting the above technical solution, the diameter of the drainage pinhole at the bottom of the reaction tank is set to 0.5~0.3mm, which can not only reduce the difficulty of hole processing and surface treatment in the hole, but also effectively control the surface tension of the liquid. At the same time, when drainage is required, the drainage holes within this size range can quickly discharge waste liquid under the application of appropriate air pressure, thereby improving drainage efficiency and reliability. This design not only reduces the complexity and cost of the equipment, but also improves the ease of operation and work efficiency.

[0011] Optionally, the air retention component includes a cavity; an upper connector is provided at the inlet end of the cavity, and a lower connector is provided at the outlet end; conical pipes are provided in the upper connector and the lower connector; the conical pipes in the upper connector and the lower connector are connected to the inner cavity of the cavity, and the small ends of the conical pipes of the upper connector and the lower connector are arranged facing each other; the lower connector is placed in the liquid contained in the waste liquid tank.

[0012] By adopting the above technical scheme, the design of the air retention component not only allows the air in the lower section of the drainage needle hole to be sealed and pressure-maintained by the surface tension of the liquid, but also can quickly discharge the liquid while providing a relatively small pressure, making the drainage process more efficient and reliable. Specifically, an upper connector is provided at the inlet end of the cavity, and a lower connector is provided at the outlet end, ensuring that the gas can enter and discharge smoothly. At the same time, a buffer accommodating chamber is formed between the upper connector and the lower connector of the cavity, which can perform gas buffering, reduce the air pressure required for drainage, and facilitate the discharge of waste liquid; conical pipes are provided in the upper connector and the lower connector, and the small ends of the two conical pipes are arranged facing each other. In the upper connector, since the aperture of the pipe at the lower end of the upper connector is the smallest, the surface tension of the liquid gradually increases during its downward flow, thereby forming a primary liquid sealing belt in the pipe at the lower end of the upper connector, blocking the air fluid in the pipe, and forming a pressure-maintaining effect on the air column at the lower end of the drainage needle hole. ; In the lower connector, the aperture of the upper end of the pipe of the lower connector is the smallest, and then the surface tension of the liquid in the upper end of the pipe of the lower connector is the largest, which will form a secondary liquid sealing belt to block the fluid of air in the pipe, and can form a pressure-maintaining effect on the air in the tube cavity. When the lower connector is placed in the liquid contained in the waste liquid tank, the liquid submerges the upper end of the lower connector and is flush with the external liquid surface. If the air wants to pass through the secondary liquid sealing belt, it needs to overcome the dual forces of the maximum surface tension of the liquid and the water pressure, which further enhances the sealing and air-maintaining effect. It should be pointed out that by adopting the air-maintaining component of the present structure, the surface tension of the liquid is used for self-sealing, which can shorten the length of the pipe immersed in the liquid, reduce the size of the waste liquid tank, and facilitate other moving operations of the waste liquid tank. Among them, the cavity adopted by the air-maintaining component can adopt conventional medical infusion pipes, and the upper connector and the lower connector can adopt conventional medical plugs, which are simple to obtain materials and have low manufacturing costs.

[0013] Optionally, the volume of the inner cavity of the cavity is not less than the volume of the reaction liquid in the reaction tank; and the outer walls of the upper connector and the lower connector are conical structures.

[0014] By adopting the above technical solution, the volume of the cavity inside the cavity is not less than the volume of the reaction liquid in the reaction tank. On the one hand, it can ensure that there is enough space in the cavity to temporarily accommodate all the reaction liquid in the reaction tank to facilitate the discharge of waste liquid. On the other hand, it can prevent the liquid in the cavity from flowing back. At the same time, the outer walls of the upper connector and the lower connector are designed with a conical structure, which can further increase the volume of the liquid holding space in the cavity inside the cavity to provide a large enough space for air pressure buffering.

[0015] Further optionally, the cavity is a bellows-shaped structure and is made of a flexible rubber material.

[0016] By adopting the above technical solution, the cavity has a bellows-like structure which can effectively improve its own deformation ability, and can further enhance the ability to reduce the pressure and buffer the gas or liquid entering the cavity, thereby facilitating the discharge of waste liquid. At the same time, the bellows-like structure can also absorb and alleviate external vibrations or impacts to a certain extent, further improving the reliability and service life of the device.

[0017] Further optionally, the pressurizing device also includes a balloon, which is connected to the output end of the lower connector, and a plurality of capillary holes are provided on the side wall of the balloon.

[0018] By adopting the above technical solution, the balloon is connected to the output end of the lower connector, and a number of capillaries are provided on the side wall of the balloon. This design allows the balloon to ensure that the inner cavity is connected to the external liquid while also utilizing the surface tension of the liquid in the capillaries to achieve a certain sealing effect on the balloon wall, thereby further improving the sealing performance of the air retention device.

[0019] Further optionally, the pressurizing device also includes a balloon, which is connected to the output end of the lower connector, and is made of a flexible rubber material, and a plurality of slits arranged along the axial direction are evenly distributed on the side wall of the balloon.

[0020] By adopting the above technical solution, the balloon is made of flexible rubber material, and the side wall of the balloon is evenly distributed with a number of axially arranged slits. This design ensures that the inner cavity of the balloon is connected with the external liquid, while also utilizing the surface tension of the liquid in the slit to achieve a certain sealing effect on the balloon wall, thereby further improving the sealing performance of the air retention device. In the process of pressurized drainage, the balloon will deform and the slit can be expanded to facilitate the discharge of waste liquid.

[0021] Further optionally, the upper connector is made of a flexible rubber material, and a lower end has a plurality of axially arranged open slits evenly distributed in the circumferential direction.

[0022] By adopting the above technical solution, the lower end of the upper connector has the ability to elastically deform, and can expand under pressure during the drainage process, thereby increasing the cross-sectional area of ​​the drainage channel and facilitating the discharge of waste liquid.

[0023] Optionally, the synthetic plate is arranged on a workbench; the pressurizing device includes a cover body, the cover body is provided with a groove adapted to the synthetic plate; a closed space for covering the reaction tank is formed between the groove and the workbench; the cover body is provided with an air inlet connected to the closed space, and the air inlet is used for connecting a compressed air pipeline.

[0024] By adopting the above technical scheme, the drainage efficiency and reliability of the DNA synthesizer can be effectively improved. Specifically, the synthesis board is arranged on the workbench, which ensures the stability of the synthesis board and avoids displacement caused by vibration or other external factors, thereby improving the overall stability of the equipment; the pressurizing device includes a cover body, and the cover body is provided with a groove adapted to the synthesis board, so that the cover body can fit the synthesis board tightly to form a sealed space, effectively preventing external gas from entering, and ensuring the stability and consistency of the internal pressure; this design can simultaneously pressurize multiple reaction tanks on the synthesis board with gas, and discharge the waste liquid in the reaction tank, greatly improving the drainage efficiency; the cover body is provided with an air inlet connected to the closed space, and the air inlet is used for compressed air pipeline connection. In this way, high-pressure gas is introduced, which can quickly increase the air pressure in the reaction tank in a short time, prompting the liquid to be quickly discharged, and improving the work efficiency. This design can simultaneously pressurize multiple reaction tanks on the synthesis board with gas, and discharge the waste liquid in the reaction tank, greatly improving the drainage efficiency. At the same time, this design is also convenient for operation and maintenance, reducing the complexity of the system.

[0025] Further optionally, a locking mechanism is provided between the cover body and the workbench, and the locking mechanism is used to fix the cover body on the workbench.

[0026] By adopting the above technical solution, the locking mechanism can ensure that the cover body is firmly fixed on the workbench, thereby effectively preventing airtightness problems during the pressurization process; specifically, the locking mechanism makes the connection between the cover body and the workbench more firm and reliable, avoiding loosening or displacement due to external interference, ensuring the stability of the gas pressure in the reaction tank, and thus improving the efficiency and reliability of waste liquid discharge.

[0027] Further optionally, the locking mechanism includes a magnetic device fixed on the workbench and an excitation body fixed on the cover body; the magnetic device includes an iron core and a conductive coil wound around the iron core.

[0028] By adopting the above technical solution, the relative position between the cover and the workbench can be effectively fixed, the sealing of the reaction tank during the pressurization process can be ensured, and the drainage efficiency and reliability can be improved. Specifically, the magnetic device generates a magnetic field through the iron core and the conductive coil wound thereon, thereby attracting the excitation body fixed on the cover to achieve a firm lock between the cover and the workbench; the excitation body is fixed on the cover and cooperates with the magnetic device to enhance the locking effect and prevent the cover from loosening due to external vibration or pressure changes. The overall design of the locking mechanism not only improves the operating convenience and safety of the equipment, but also ensures a closed environment during the pressurization process, avoids air leakage, and improves the stability and work efficiency of the overall system.

[0029] In summary, the present application includes at least one of the following beneficial technical effects: The present application utilizes the supporting effect of the liquid surface tension and the air pressure of the lower section, so that the liquid can be stably retained in the reaction tank under normal air pressure, reducing the dependence on the solenoid valve, reducing the equipment cost and maintenance complexity, and can use a relatively small air pressure to discharge the liquid in the reaction tank, reducing the difficulty of liquid discharge and improving the efficiency of liquid discharge; The design of the air retaining component of the present application not only allows the air in the lower section of the drainage needle hole to be sealed and pressure-maintained by the surface tension of the liquid, but also allows the liquid to be rapidly discharged while providing a relatively small pressure, making the drainage process more efficient and reliable; The gas retaining component of the present application is simple in material, low in manufacturing cost, and uses the surface tension of the liquid for self-sealing, which can shorten the length of the pipeline immersed in the liquid, reduce the size of the waste liquid tank, and facilitate other moving operations of the waste liquid tank; When drainage is required, the present application increases the air pressure in multiple reaction tanks simultaneously through a pressurizing device, so that the liquid overcomes its own surface tension and the supporting force of the lower air pressure and is discharged from the drainage pinhole. This method is simple and efficient, and improves the drainage speed and reliability. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 is a schematic diagram of the arrangement of the pressurized liquid discharge device applied to the DNA synthesizer described in the first embodiment; Figure 2 is a schematic structural diagram of the air retaining assembly described in Example 1; Figure 3 is a schematic diagram of the sealing state of the upper connector described in Example 1; Figure 4 is a schematic diagram of the sealing state of the lower connector described in Example 1; Figure 5 It is a schematic diagram of the arrangement of the pressurized liquid discharge device applied to the DNA synthesizer described in the second embodiment; Figure 6 is a schematic structural diagram of the gas retaining assembly described in Example 2; Figure 7 is a structural schematic diagram of the locking mechanism described in Example 2; Figure 8 is a schematic diagram of the structure of the balloon described in Example 3; Fig. 9 It is a schematic diagram of the structure of the balloon described in Example 4.

[0031] In the figure: 1. synthetic board; 11. reaction tank; 12. drainage pinhole; 2. air retaining component; 21. cavity; 22. upper connector; 221. opening seam; 23. lower connector; 24. conical pipe; 3. balloon; 31. capillary pore; 32. seam; 4. pressurizing device; 41. cover plate; 411. groove; 412. air inlet; 42. sealing gasket; 5. locking mechanism; 51. exciter; 52. iron core; 53. coil; 6. workbench; 7. waste liquid tank. DETAILED DESCRIPTION

[0032] The following will clearly and completely describe the technical solutions of the embodiments of the present application in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments of the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present application. Example

[0033] Reference Figure 1 The embodiment of the present application discloses a pressurized liquid discharge device applied to a DNA synthesizer, comprising a synthesis plate 1, an air retaining component 2, a pressurizing device 4 and a waste liquid tank 7, wherein a plurality of reaction tanks 11 for synthesizing DNA are evenly distributed on the synthesis plate 1, the reaction tanks 11 are arranged in a vertical direction, and a liquid discharge pinhole 12 is provided at the bottom of the reaction tank 11; the liquid in the reaction tank 11 is retained in the reaction tank 11 at the input end of the liquid discharge pinhole 12 by the support of its own surface tension and the air pressure of the lower section; the air retaining component 2 is connected to the output end of the liquid discharge pinhole 12 through a pipeline, and the air below the liquid discharge pinhole 12 is sealed by the surface tension of the liquid, and the supporting air pressure is provided for the liquid tension interface at the input end of the liquid discharge pinhole 12. The pressurizing device 4 is used to increase the air pressure in the reaction tank 11, drive the liquid in the reaction tank 11 to overcome its own surface tension and the supporting force of the air pressure of the lower section, and discharge from the liquid discharge pinhole 12. The waste liquid tank 7 is used to collect the liquid discharged from the reaction tank 11.

[0034] Specifically, refer to Figure 1The synthetic board 1 is arranged on the workbench 6, and the pressurizing device 4 includes a cover body, and a groove 411 adapted to the synthetic board 1 is provided on the cover body, and a closed space for covering the reaction tank 11 is formed between the groove 411 and the workbench 6; an air inlet 412 connected to the closed space is provided on the cover body, and the air inlet 412 is used for connecting a compressed air pipeline; by introducing high-pressure gas in this way, the air pressure in the reaction tank 11 can be quickly increased in a short time, so that the liquid is quickly discharged, thereby improving the working efficiency; in order to reduce the weight of the device, the cover plate 41 can be made of plastic or aluminum alloy; in order to improve the sealing performance of the cover body, a sealing gasket 42 is provided on the edge where the cover body contacts the workbench 6; and in order to facilitate the control of the temperature of the liquid in the reaction tank 11 to accelerate the reaction process, the synthetic board 1 is made of metal material, utilizing the good thermal conductivity of metal.

[0035] Reference Figure 1 The bottom of the reaction tank 11 is in an arc structure, which helps to reduce the amount of liquid residue and improve the drainage efficiency; the diameter of the drainage pinhole 12 is 0.5~0.3mm. This size can not only reduce the difficulty of hole processing, but also effectively control the surface tension of the liquid, ensuring that the waste liquid can be quickly discharged when drainage is needed.

[0036] Reference Figures 2~4 The gas retaining component 2 includes a cavity 21, an upper connector 22 is provided at the inlet end of the cavity 21, and a lower connector 23 is provided at the outlet end, wherein the upper connector 22 and the lower connector 23 can be made of plastic material, and the cavity 21 can be made of a conventional transparent heat shrink tube; the upper connector 22 is connected to the output end of the drainage pinhole 12 through a pipeline, and the lower connector 23 is placed in the liquid contained in the waste liquid tank 7. When working, the cavity 21 forms a buffer accommodating chamber between the upper connector 22 and the lower connector 23, which can perform gas buffering, reduce the air pressure required for drainage, and facilitate the discharge of waste liquid.

[0037] Reference Figures 2~4 The upper connector 22 and the lower connector 23 are both provided with a tapered pipe 24, and the small ends of the two tapered pipes 24 are arranged facing each other. When working, in the upper connector 22, since the aperture of the pipe at the lower end of the upper connector 22 is the smallest, the surface tension of the liquid gradually increases during the downward flow, thereby forming a primary liquid sealing belt in the pipe at the lower end of the upper connector 22, blocking the fluid of the air in the pipe, and forming a pressure-maintaining effect on the air column at the lower end of the drainage pinhole 12, see Figure 3; In the lower connector 23, the aperture of the pipe at the upper end of the lower connector 23 is the smallest, and thus the surface tension of the liquid in the pipe at the upper end of the lower connector 23 is the largest, which will form a secondary liquid sealing belt to block the fluid of the air in the pipe, and can form a pressure-maintaining effect on the air in the lumen. When the lower connector 23 is placed in the liquid contained in the waste liquid tank 7, the liquid covers the upper end of the lower connector 23 and is flush with the external liquid surface. If the air wants to pass through the secondary liquid sealing belt, it needs to overcome the dual forces of the maximum liquid surface tension and the water pressure, which further enhances the sealing and air-maintaining effect. For details, see Figure 4 .

[0038] Reference Figures 2~4 The outer walls of the upper connector 22 and the lower connector 23 are tapered, which helps to increase the volume of the liquid holding space in the inner cavity of the cavity 21, thereby providing sufficient space for air pressure buffering; and the volume of the inner cavity of the cavity 21 should not be less than the volume of the reaction liquid in the reaction tank 11 when designing, so as to ensure that there is enough space in the cavity 21 to temporarily hold all the reaction liquid in the reaction tank 11 to prevent liquid backflow.

[0039] The implementation principle of this embodiment is as follows: in the initial state, the liquid in the reaction tank 11 is supported by its own surface tension and the air pressure of the lower section at the input end of the drainage needle hole 12 and remains in the reaction tank 11. The air retaining component 2 is connected to the output end of the drainage needle hole 12 through a pipeline, and the air below the drainage needle hole 12 is sealed by the surface tension of the liquid, providing supporting air pressure for the liquid tension interface at the input end of the drainage needle hole 12; when drainage is required, compressed air is introduced through the pressurizing device 4 to increase the air pressure in the reaction tank 11. At this time, the liquid in the reaction tank 11 overcomes its own surface tension and the supporting force of the air pressure of the lower section, and is discharged from the drainage needle hole 12. Example

[0040] Reference Figures 5 to 7 , the difference between this embodiment and the first embodiment is that the cavity 21 is a bellows-shaped structure and is made of a flexible rubber material, such as chloroprene rubber; the upper connector 22 is made of a flexible rubber material, and a plurality of axially arranged opening slits 221 are evenly distributed around the lower end. This design enables the lower end of the upper connector 22 to have the ability to elastically deform, and can be compressed and expanded during the drainage process, thereby increasing the cross-sectional area of ​​the drainage channel and facilitating the discharge of waste liquid; and in order to ensure that the cover body is firmly fixed on the workbench 6, a locking mechanism 5 can be provided between the cover body and the workbench 6, and the locking mechanism 5 includes a magnetic device fixed on the workbench 6 and an excitation body 51 fixed on the cover body; the excitation body 51 is symmetrically arranged on both sides of the cover body and can be fixed to the cover body by bolts. When working, the magnetic device generates a magnetic field through the iron core 52 and the conductive coil 53 wound thereon, attracting the excitation body 51 fixed on the cover body, so as to achieve a firm locking of the cover body and the workbench 6.

[0041] The implementation principle of this embodiment is as follows: the cavity 21 is a bellows-shaped structure, which can effectively improve its own deformation ability, and can further improve the ability to decompress and buffer the gas or liquid entering the cavity 21, so as to facilitate the discharge of waste liquid. At the same time, the bellows-shaped structure can also absorb and alleviate external vibration or impact to a certain extent, further improving the reliability and service life of the device; the design of the opening slit 221 on the upper connector 22 enables the lower end of the upper connector 22 to have the ability of elastic deformation, which can be compressed and expanded during the discharge process, thereby increasing the cross-sectional area of ​​the discharge channel and facilitating the discharge of waste liquid; the locking mechanism 5 can ensure that the cover body is firmly fixed on the workbench 6, thereby effectively preventing the airtightness problem during the pressurization process; specifically, the locking mechanism 5 makes the connection between the cover body and the workbench 6 more firm and reliable, avoids loosening or displacement caused by external interference, ensures the stability of the gas pressure in the reaction tank 11, and thus improves the efficiency and reliability of waste liquid discharge. Example

[0042] Reference Figure 8 The difference between this embodiment and the first embodiment is that the pressurizing device 4 includes a balloon 3, the balloon 3 is connected to the output end of the lower connector 23, and a plurality of capillary holes 31 are provided on the side wall of the balloon 3, wherein the balloon 3 can be made of hard plastic, and the diameter of the capillary holes 31 is 0.5-0.3 mm.

[0043] The implementation principle of this embodiment is: the capillaries 31 provided on the side wall of the balloon 3 ensure that the inner cavity of the balloon 3 is connected with the external liquid, and at the same time, the surface tension of the liquid in the capillaries 31 can be used to achieve a certain sealing effect on the wall of the balloon 3, thereby further improving the sealing performance of the air retention device. Example

[0044] Reference Fig. 9 The difference between this embodiment and the first embodiment is that the pressurizing device 4 includes a balloon 3, which is connected to the output end of the lower connector 23. The balloon 3 is made of a flexible rubber material, and the side wall of the balloon 3 is evenly distributed in the circumferential direction with a plurality of slits 32 arranged along the axial direction.

[0045] The implementation principle of this embodiment is: this design can not only increase the surface tension of the liquid, but also deform during the process of pressurized drainage, so that the slit 32 can be expanded to facilitate the discharge of waste liquid.

[0046] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit it. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the technical solutions of the embodiments of the present application.

Claims

1. A pressurized liquid discharge device for use in a DNA synthesizer, characterized in that: The invention comprises a synthesis plate (1), an air retaining component (2), a pressurizing device (4) and a waste liquid tank (7), wherein a plurality of reaction tanks (11) for synthesizing DNA are evenly distributed on the synthesis plate (1), wherein the reaction tanks (11) are arranged in a vertical direction, and a drainage pinhole (12) is provided at the bottom of the reaction tank (11), and the liquid in the reaction tank (11) is retained in the reaction tank (11) at the input end of the drainage pinhole (12) by the support of its own surface tension and the air pressure of the lower section of the liquid; the air retaining component (2) is connected to the output end of the drainage pinhole (12) through a pipeline, and is used for sealing the air below the drainage pinhole (12) by utilizing the surface tension of the liquid, so as to provide supporting air pressure for the liquid tension interface at the input end of the drainage pinhole (12); The pressurizing device (4) is used to increase the air pressure in the reaction tank (11), driving the liquid in the reaction tank (11) to overcome its own surface tension and the supporting force of the air pressure in the lower section, and to be discharged from the liquid discharge pinhole (12); the waste liquid tank (7) is used to water-seal the output end of the air retaining component (2) and collect the liquid discharged from the reaction tank (11).

2. The pressurized liquid discharge device for use in a DNA synthesizer according to claim 1, characterized in that: The bottom of the reaction tank (11) is in an arc structure.

3. The pressurized liquid discharge device for use in a DNA synthesizer according to claim 1, characterized in that: The diameter of the drainage needle hole (12) is 0.5-0.3 mm.

4. The pressurized liquid discharge device for use in a DNA synthesizer according to claim 1, characterized in that: The gas retaining component (2) comprises a cavity (21); an upper connector (22) is provided at the inlet end of the cavity (21), and a lower connector (23) is provided at the outlet end; a conical pipe (24) is provided in each of the upper connector (22) and the lower connector (23); the conical pipes (24) in the upper connector (22) and the lower connector (23) are both communicated with the inner cavity of the cavity (21), and the small ends of the conical pipes (24) of the upper connector and the lower connector are arranged facing each other; the lower connector (23) is placed in the liquid contained in the waste liquid tank (7).

5. The pressurized liquid discharge device for use in a DNA synthesizer according to claim 4, characterized in that: The volume of the inner cavity of the cavity (21) is not less than the volume of the reaction liquid in the reaction tank (11); and the outer walls of the upper connector (22) and the lower connector (23) are in a conical structure.

6. The pressurized liquid discharge device for use in a DNA synthesizer according to claim 4, characterized in that: The cavity (21) is in the form of a corrugated tube and is made of a flexible rubber material.

7. The pressurized liquid discharge device for use in a DNA synthesizer according to claim 4, characterized in that: The pressurizing device (4) further comprises a balloon (3), wherein the balloon (3) is connected to the output end of the lower connector (23), and a plurality of capillary holes (31) are provided on the side wall of the balloon (3).

8. The pressurized liquid discharge device for use in a DNA synthesizer according to claim 4, characterized in that: The pressurizing device (4) further comprises a balloon (3), wherein the balloon (3) is connected to the output end of the lower connector (23), the balloon (3) is made of a flexible rubber material, and a plurality of slits (32) arranged along the axial direction are evenly distributed on the side wall of the balloon (3) in the circumferential direction.

9. The pressurized liquid discharge device for use in a DNA synthesizer according to claim 4, characterized in that: The upper connecting head (22) is made of a flexible rubber material, and has a plurality of axially arranged opening slits (221) evenly distributed in the circumferential direction at the lower end.

10. The pressurized liquid discharge device for use in a DNA synthesizer according to claim 1, characterized in that: The synthetic plate (1) is arranged on a workbench (6); the pressurizing device (4) comprises a cover body, the cover body being provided with a groove (411) adapted to the synthetic plate (1); a closed space for covering the reaction tank (11) is formed between the groove (411) and the workbench (6); the cover body is provided with an air inlet (412) communicating with the closed space, the air inlet (412) being used for connecting a compressed air pipeline.

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