Saline-alkali soil modifier added with biological enzyme preparation and preparation method of saline-alkali soil modifier

Abstract

The invention discloses a saline-alkali soil modifier added with a biological enzyme preparation and a preparation method of the saline-alkali soil modifier, and belongs to the technical field of saline-alkali soil improvement. The modifier comprises the following raw materials in parts by weight: 10-20 parts of cellulase, 3-8 parts of protease, 1-5 parts of urease, 5-15 parts of xylanase, 4-10 parts of phosphatase, 3-9 parts of wormcast, 10-18 parts of a stabilizer, 3-8 parts of wood vinegar and 10-20 parts of a carrier. The effect of enhancing the durability of saline-alkali soil improvement is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of saline-alkali land improvement, in particular to a saline-alkali land improver with added bioenzyme preparation and a preparation method thereof. Background Art

[0002] Soil salinization is a common environmental problem in the world. Some components in salinized soil will damage the growth and development of crops and are an important factor limiting crop yields. Soil salinization refers to a form of soil formed by the concentration of salts in the land under certain natural conditions. When the content of soluble salts or water-soluble salts in the surface or sub-surface of the soil exceeds 0.1%, or the alkalinity of the soil alkaline layer exceeds 5%, it is considered soil salinization. Soil salinization is the main cause of soil degradation and reduced crop yield and quality.

[0003] The characteristics of saline-alkali land such as high salinity, poor structure and low nutrients have made it difficult to improve and utilize it for a long time. Most of the existing methods for improving saline-alkali land soil include the use of organic waste, biochar, humic acid and other methods, but the improvement effects are ultimately not lasting. Summary of the invention

[0004] In order to solve the problems in the prior art, the present invention provides a saline-alkali land improver with added biological enzyme preparation and a preparation method thereof, which enhances the durability of saline-alkali land improvement, and the composite enzyme preparation of the present application has higher compatibility with saline-alkali land.

[0005] The present invention provides a saline-alkali land improver with added bio-enzyme preparation and a preparation method thereof, which adopts the following technical scheme: A saline-alkali land improver with added biological enzyme preparations, the improver comprising the following raw materials in parts by weight: 10-20 parts of cellulase, 3-8 parts of protease, 1-5 parts of urease, 5-15 parts of xylanase, 4-10 parts of phosphatase, 3-9 parts of earthworm castings, 10-18 parts of stabilizer, 3-8 parts of wood vinegar, and 10-20 parts of carrier.

[0006] Preferably, the cellulase comprises 12-17 parts, protease comprises 4.5-6 parts, urease comprises 2-4 parts, xylanase comprises 8-12 parts, phosphatase comprises 5-8 parts, earthworm castings comprises 4-7 parts, stabilizer comprises 12-16 parts, wood vinegar comprises 4-6.5 parts, and carrier comprises 12-18 parts.

[0007] By adopting the above technical scheme, the composite enzyme composed of cellulase, protease, urease, xylanase and phosphatase in the present application has high compatibility with the soil, and can effectively improve the durability of the composite enzyme preparation for soil improvement. In addition, the addition of wood vinegar in the improver and its use in combination with the composite enzyme can make more single particles in the soil gather together to form aggregates, significantly reduce the soil pH value, and the formation of aggregates will increase the soil porosity, reduce the soil compaction, increase the oxygen content in the soil, and promote the development of plant roots. In addition, the combination of the composite enzyme with wood vinegar and earthworm castings also plays a certain regulatory role on saline-alkali soil and alkalinity, so that the content of organic matter in the soil is efficiently released, phosphorus and carbon elements in the soil are activated, and the growth and development of plants in saline-alkali soil are promoted, so that the plant yield and quality are significantly changed.

[0008] Preferably, the phosphatase is acid phosphatase.

[0009] Preferably, the stabilizer is sodium polyacrylate or polyvinyl pyrrolidone.

[0010] Preferably, the carrier consists of diatomaceous earth and chitosan in a weight ratio of 1:1.

[0011] Preferably, the particle size of the diatomaceous earth is 50-60 mesh, and the particle size of the chitosan is 80-100 mesh.

[0012] The second aspect of the present invention provides a method for preparing the above-mentioned saline-alkali land improver with added bio-enzyme preparation, comprising the following preparation steps: S1, mixing cellulase, protease, urease, xylanase and phosphatase according to weight parts to obtain a composite enzyme, and then adding a buffer solution to prepare a composite enzyme solution; S2, after the carrier and the stabilizer are mixed, the complex enzyme solution obtained in step S1 is added, and then the buffer solution 2 is added to solidify for 5-6 hours at 23±1°C and a rotation speed of 200±10r / min, and then filtered, and then mixed with wood vinegar and earthworm castings and dried to obtain an improver.

[0013] Preferably, the buffer solution 1 and the buffer solution 2 are both citric acid-sodium citrate solutions, the pH of the buffer solution 1 is 4.5-5.5, and the pH of the buffer solution 2 is 4-5.

[0014] Preferably, in step S2, buffer solution II and alanine are added and solidified at 23±1°C and a rotation speed of 200±10r / min for 5-6h, then filtered, and then stirred and mixed with wood vinegar and earthworm castings and dried to obtain the improver.

[0015] Preferably, the amount of alanine added is 1-1.5% of the weight of the complex enzyme.

[0016] In summary, the present invention has the following beneficial effects: 1. The composite enzyme composed of cellulase, protease, urease, xylanase and phosphatase in the present application has high compatibility with soil, and can effectively improve the durability of the composite enzyme preparation for soil improvement. In addition, the addition of wood vinegar in the improver and its use in combination with the composite enzyme can make more single particles in the soil gather together to form aggregates, significantly reduce the soil pH value, and also play a certain regulatory role on saline-alkali soil and alkalinity, so that the content of organic matter in the soil is efficiently released, phosphorus and carbon elements in the soil are activated, and the growth and development of plants in saline-alkali soil are promoted, so that plant yield and quality change more obviously.

[0017] 2. When the carrier in this application is a mixture of diatomaceous earth and chitosan, the stability of the complex enzyme preparation in the soil can be improved, so that the organic enzyme content in the soil maintains a high stability and activity; and the selection of two different types of carriers provides suitable adsorption sites for the attachment of complex enzyme molecules, maintains a high adsorption stability, and thus ensures the stability of the complex enzyme molecules in the soil. In addition, this application further defines the particle size of the chitosan carrier and the diatomaceous earth carrier, provides more active sites for the attachment of the complex enzyme molecules, makes the complex enzyme molecules more easily attached to the carrier surface, thereby improving the activity of the immobilized complex enzyme.

[0018] 3. During the preparation of the improver of the present application, the activity of the complex enzyme obtained after adding alanine is higher, which effectively improves the improvement effect and durability of the improver on the soil. DETAILED DESCRIPTION

[0019] The present invention is further described in detail below with reference to the embodiments.

[0020] The raw materials used in this application are all commercially available.

[0021] Cellulase: CAS number is 9012-54-8, enzyme activity is 10000U / g; The protease used is alkaline protease: CAS number is 37259-58-8, and the enzyme activity is 100,000 U / g; Urease: CAS number is 9002-13-5, enzyme activity is 1.02U / mg; Xylanase: CAS number is 9025-57-4, enzyme activity ≥ 6000U / mg; Acid phosphatase: CAS number is 9001-77-8, enzyme activity is 0.5-3U / mg.

[0022] A saline-alkali land improver with added biological enzyme preparations, the improver comprising the following raw materials in parts by weight: 10-20 parts of cellulase, 3-8 parts of protease, 1-5 parts of urease, 5-15 parts of xylanase, 4-10 parts of phosphatase, 3-9 parts of earthworm castings, 10-18 parts of stabilizer, 3-8 parts of wood vinegar, and 10-20 parts of carrier.

[0023] Preferably, the cellulase comprises 12-17 parts, protease comprises 4.5-6 parts, urease comprises 2-4 parts, xylanase comprises 8-12 parts, phosphatase comprises 5-8 parts, earthworm castings comprises 4-7 parts, stabilizer comprises 12-16 parts, wood vinegar comprises 4-6.5 parts, and carrier comprises 12-18 parts.

[0024] Preferably, the phosphatase is acid phosphatase.

[0025] Preferably, the stabilizer is sodium polyacrylate or polyvinyl pyrrolidone.

[0026] Preferably, the carrier consists of diatomaceous earth and chitosan in a weight ratio of 1:1.

[0027] Preferably, the particle size of the diatomaceous earth is 50-60 mesh, and the particle size of the chitosan is 80-100 mesh.

[0028] The second aspect of the present invention provides a method for preparing the above-mentioned saline-alkali land improver with added bio-enzyme preparation, comprising the following preparation steps: S1, mixing cellulase, protease, urease, xylanase and phosphatase according to weight parts to obtain a composite enzyme, and then adding a buffer solution to prepare a composite enzyme solution; S2, after the carrier and the stabilizer are mixed, the complex enzyme solution obtained in step S1 is added, and then the buffer solution 2 is added to solidify for 5-6 hours at 23±1°C and a rotation speed of 200±10r / min, and then filtered, and then mixed with wood vinegar and earthworm castings and dried to obtain an improver.

[0029] Preferably, the buffer solution 1 and the buffer solution 2 are both citric acid-sodium citrate solutions, the pH of the buffer solution 1 is 4.5-5.5, and the pH of the buffer solution 2 is 4-5.

[0030] Preferably, in step S2, buffer solution II and alanine are added and solidified at 23±1°C and a rotation speed of 200±10r / min for 5-6h, then filtered, and then stirred and mixed with wood vinegar and earthworm castings and dried to obtain the improver.

[0031] Preferably, the amount of alanine added is 1-1.5% of the weight of the complex enzyme. Example 1

[0032] A method for preparing a saline-alkali land improver with added biological enzyme preparations comprises the following preparation steps: S1, 1 kg of cellulase, 0.3 kg of protease, 0.1 kg of urease, 0.5 kg of xylanase, and 0.4 kg of acid phosphatase were mixed to obtain a composite enzyme, and then a citric acid-sodium citrate solution with a pH of 4.5 was added to prepare a composite enzyme solution with a concentration of 1.0 kg / L; S2, 1 kg of carrier (a mixture of diatomaceous earth and chitosan in a weight ratio of 1:1) and 1 kg of stabilizer (sodium polyacrylate) were mixed, and the complex enzyme solution obtained in step S1 was added, and then 1 kg of citric acid-sodium citrate solution with a pH of 4 was added, and solidified for 5 hours at 23±1°C and a rotation speed of 200±10r / min, and then filtered, and then mixed with 0.3 kg of wood vinegar and 0.3 kg of earthworm castings, and then dried to obtain an improver. Example 2

[0033] A method for preparing a saline-alkali land improver with added biological enzyme preparations comprises the following preparation steps: S1, 1.2 kg of cellulase, 0.45 kg of protease, 0.2 kg of urease, 0.8 kg of xylanase, and 0.5 kg of acid phosphatase were mixed to obtain a composite enzyme, and then a citric acid-sodium citrate solution with a pH of 4.5 was added to prepare a composite enzyme solution with a concentration of 1.0 kg / L; S2, 1.2 kg of carrier (a mixture of diatomaceous earth and chitosan in a weight ratio of 1:1) and 1.2 kg of stabilizer (sodium polyacrylate) were mixed, and the complex enzyme solution obtained in step S1 was added, and then 1 kg of citric acid-sodium citrate solution with a pH of 4 was added and solidified for 5 hours at 23±1°C and a rotation speed of 200±10r / min, and then filtered, and then mixed with 0.4 kg of wood vinegar and 0.4 kg of earthworm castings, and then dried to obtain an improver. Example 3

[0034] A method for preparing a saline-alkali land improver with added biological enzyme preparations comprises the following preparation steps: S1, 1.5 kg of cellulase, 0.5 kg of protease, 0.3 kg of urease, 1.0 kg of xylanase, and 0.6 kg of acid phosphatase were mixed to obtain a composite enzyme, and then a citric acid-sodium citrate solution with a pH of 4.5 was added to prepare a composite enzyme solution with a concentration of 1.0 kg / L; S2, 1.5 kg of carrier (a mixture of diatomaceous earth and chitosan in a weight ratio of 1:1) and 1.4 kg of stabilizer (sodium polyacrylate) were mixed, and the complex enzyme solution obtained in step S1 was added, and then 1 kg of citric acid-sodium citrate solution with a pH of 4 was added and solidified at 23±1°C and a rotation speed of 200±10 r / min for 6 hours, and then filtered, and then mixed with 0.5 kg of wood vinegar and 0.55 kg of earthworm castings, and then dried to obtain an improver. Example 4

[0035] A method for preparing a saline-alkali land improver with added biological enzyme preparations comprises the following preparation steps: S1, 1.7 kg of cellulase, 0.6 kg of protease, 0.4 kg of urease, 1.2 kg of xylanase, and 0.8 kg of acid phosphatase were mixed uniformly to obtain a composite enzyme, and then a citric acid-sodium citrate solution with a pH of 4.5 was added to prepare a composite enzyme solution with a concentration of 1.0 kg / L; S2, 1.8 kg of carrier (a mixture of diatomaceous earth and chitosan in a weight ratio of 1:1) and 1.6 kg of stabilizer (sodium polyacrylate) were mixed and added to the complex enzyme solution obtained in step S1, and then 1.5 kg of citric acid-sodium citrate solution with a pH of 4 was added and solidified for 5 hours at 23±1°C and a rotation speed of 200±10 r / min, and then filtered, and then mixed with 0.65 kg of wood vinegar and 0.7 kg of earthworm castings, and then dried to obtain an improver. Example 5

[0036] A method for preparing a saline-alkali land improver with added biological enzyme preparations comprises the following preparation steps: S1, 2 kg of cellulase, 0.8 kg of protease, 0.5 kg of urease, 1.5 kg of xylanase and 1 kg of acid phosphatase were mixed to obtain a composite enzyme, and then a citric acid-sodium citrate solution with a pH of 4.5 was added to prepare a composite enzyme solution with a concentration of 1.0 kg / L; S2, 2kg of carrier (a mixture of diatomaceous earth and chitosan in a weight ratio of 1:1) and 1.8kg of stabilizer (sodium polyacrylate) were mixed, and the complex enzyme solution obtained in step S1 was added, and then 1.5kg of citric acid-sodium citrate solution with a pH of 4 was added, and the mixture was cured for 6h at 23±1°C and a rotation speed of 200±10r / min, and then filtered, and then mixed with 0.8kg of wood vinegar and 0.9kg of earthworm castings, and then dried to obtain an improver. Example 6

[0037] A method for preparing a saline-alkali land improver with added bio-enzyme preparations is different from that of Example 3 in that the pH of the citric acid-sodium citrate solution in step S1 is 5.5, and the rest is the same as that of Example 3. Example 7

[0038] A method for preparing a saline-alkali land improver with added bio-enzyme preparations is different from that of Example 3 in that the pH of the citric acid-sodium citrate solution in step S2 is 4.5, and the rest is the same as that of Example 3. Example 8

[0039] A method for preparing a saline-alkali land improver with added bio-enzyme preparations is different from that of Example 3 in that the pH of the citric acid-sodium citrate solution in step S2 is 5, and the rest is the same as that of Example 3. Example 9

[0040] A method for preparing a saline-alkali land improver with added biological enzyme preparations comprises the following preparation steps: S1, 1.5 kg of cellulase, 0.5 kg of protease, 0.3 kg of urease, 1.0 kg of xylanase, and 0.6 kg of acid phosphatase were mixed to obtain a composite enzyme, and then a citric acid-sodium citrate solution with a pH of 4.5 was added to prepare a composite enzyme solution with a concentration of 1.0 kg / L; S2, 1.5kg of carrier (a mixture of diatomaceous earth and chitosan in a weight ratio of 1:1) and 1.4kg of stabilizer (sodium polyacrylate) were mixed, and then the complex enzyme solution obtained in step S1 was added, and then 1kg of citric acid-sodium citrate solution with a pH of 4.5 and 0.039kg of alanine (the amount of alanine added was 1% of the total amount of the complex enzyme) were added, and the mixture was cured at 23±1°C and a rotation speed of 200±10r / min for 6h, and then filtered, and then mixed with 0.5kg of wood vinegar and 0.55kg of earthworm castings, and then dried to obtain an improver. Example 10

[0041] A method for preparing a saline-alkali land improver with added biological enzyme preparations comprises the following preparation steps: S1, 1.5 kg of cellulase, 0.5 kg of protease, 0.3 kg of urease, 1.0 kg of xylanase, and 0.6 kg of acid phosphatase were mixed to obtain a composite enzyme, and then a citric acid-sodium citrate solution with a pH of 4.5 was added to prepare a composite enzyme solution with a concentration of 1.0 kg / L; S2, 1.5kg of carrier (a mixture of diatomaceous earth and chitosan in a weight ratio of 1:1) and 1.4kg of stabilizer (sodium polyacrylate) were mixed, and then the complex enzyme solution obtained in step S1 was added, and then 1kg of citric acid-sodium citrate solution with a pH of 4.5 and 0.0468kg of alanine (the amount of alanine added was 1.2% of the total amount of the complex enzyme) were added, and the mixture was cured at 23±1°C and a rotation speed of 200±10r / min for 6h, and then filtered, and then mixed with 0.5kg of wood vinegar and 0.55kg of earthworm castings, and then dried to obtain an improver. Embodiment 11

[0042] A method for preparing a saline-alkali land improver with added biological enzyme preparations comprises the following preparation steps: S1, 1.5 kg of cellulase, 0.5 kg of protease, 0.3 kg of urease, 1.0 kg of xylanase, and 0.6 kg of acid phosphatase were mixed to obtain a composite enzyme, and then a citric acid-sodium citrate solution with a pH of 4.5 was added to prepare a composite enzyme solution with a concentration of 1.0 kg / L; S2, 1.5kg of carrier (a mixture of diatomaceous earth and chitosan in a weight ratio of 1:1) and 1.4kg of stabilizer (sodium polyacrylate) were mixed, and then the complex enzyme solution obtained in step S1 was added, and then 1kg of citric acid-sodium citrate solution with a pH of 4.5 and 0.0585kg of alanine (the amount of alanine added was 1.5% of the total amount of the complex enzyme) were added, and the mixture was cured at 23±1°C and a rotation speed of 200±10r / min for 6h, and then filtered, and then mixed with 0.5kg of wood vinegar and 0.55kg of earthworm castings, and then dried to obtain an improver.

[0043] Comparative Example 11-1 A method for preparing a saline-alkali land improver with added bio-enzyme preparation, wherein an equal amount of bentonite is used instead of diatomaceous earth, and the rest are the same as in Example 11.

[0044] Comparative Example 11-2 A method for preparing a saline-alkali land improver with added bioenzyme preparation, wherein an equal amount of trehalose is used to replace alanine, and the rest are the same as those in Example 11.

[0045] Comparative Example 1 A method for preparing a saline-alkali land improver with added biological enzyme preparations is different from that of Example 3 in that the complex enzyme lacks acid phosphatase, and the rest is the same as that of Example 3.

[0046] Comparative Example 2 A method for preparing a saline-alkali land improver with added biological enzyme preparations is different from that of Example 3 in that the complex enzyme lacks xylanase, and the rest is the same as that of Example 3.

[0047] Comparative Example 3 A method for preparing a saline-alkali land improver with added bio-enzyme preparations is different from that in Example 3 in that wood vinegar is not added in step S2, and the rest is the same as in Example 3.

[0048] Comparative Example 4 A method for preparing a saline-alkali land improver with added bio-enzyme preparations is different from that of Example 3 in that the amount of carrier added in step S2 is 2.5 kg, and the rest is the same as that of Example 3.

[0049] Performance Testing The modifiers obtained in the above embodiments and comparative examples were added to saline-alkali soil for experiments. Specifically, saline-alkali soil was added to a plastic pot for potting, the upper diameter of the plastic pot was 26 cm, the lower diameter was 20 cm, and the height was 19 cm. The experimental saline-alkali soil was air-dried, passed through a 2 mm sieve, and divided into pots. Each pot was filled with 5 kg of saline-alkali soil and 500 g of modifier. After mixing evenly, the pots were filled. Crops were planted one week later. The pH value of the soil and the content of soil organic matter, hydrolyzable nitrogen, available phosphorus, and available potassium were measured one week later. The soil moisture content was maintained at 60% of the field water holding capacity. The pakchoi seeds were repeatedly washed with distilled water, placed in a constant temperature incubator 24 hours before potting, and the surviving seeds were screened. 15 seeds were sown in each pot, and the number of pakchoi sprouts was recorded after 5 days to ensure that the amount of deionized water used in each group was the same during each watering. The pakchoi seeds were harvested after 60 days of cultivation, and the plant height of the pakchoi was measured. The measurement results are shown in Table 1. The improved soil was collected, air-dried, and sieved, and the pH value, organic matter content, hydrolyzable nitrogen, available phosphorus, and available potassium content in the soil were measured. The test results are shown in Table 2.

[0050] The physical and chemical properties of the saline-alkali soil used in the experiment are: pH 9.11, organic matter 11.63g / kg, hydrolyzable nitrogen 42.46mg / kg, available phosphorus 20.56mg / kg, and available potassium 159.66mg / kg.

[0051] Table 1 Test results of improver application performance project Germination amount Plant heightcm pH Organic matter g / kg Hydrolyzable nitrogen mg / kg Available phosphorus mg / kg Available potassium mg / kg Example 1 11 21.36 8.64 22.32 81.31 26.57 172.62 Example 2 12 22.45 8.61 22.46 82.65 26.88 174.54 Example 3 12 22.87 8.52 22.59 84.37 26.92 177.32 Example 4 12 22.86 8.51 22.58 84.26 26.92 177.41 Example 5 12 22.81 8.51 22.55 84.12 26.89 177.26 Example 6 12 22.85 8.53 22.51 84.29 26.90 177.16 Example 7 12 22.96 8.48 22.87 85.24 26.99 179.62 Example 8 12 22.95 8.50 22.80 84.98 26.95 178.96 Example 9 14 24.38 8.29 24.54 96.34 28.34 198.54 Example 10 15 26.54 8.23 25.33 99.83 28.62 202.36 Embodiment 11 15 26.49 8.23 25.46 99.45 28.66 201.98 Comparative Example 11-1 14 24.21 8.39 23.21 92.47 27.23 190.16 Comparative Example 11-2 14 24.63 8.41 23.54 93.65 27.32 192.34 Control group 5 15.56 9.09 11.63 42.46 20.56 159.66 Comparative Example 1 10 18.65 8.72 20.62 64.32 25.32 168.54 Comparative Example 2 10 17.58 8.74 20.41 64.23 25.13 167.41 Comparative Example 3 9 17.42 8.76 19.68 62.75 24.98 165.21 Comparative Example 4 10 19.36 8.81 19.46 62.14 24.87 165.32 The control group was saline-alkali soil without adding amendments.

[0052] As can be seen from Table 1, after the improver obtained in the embodiment of the present application was applied to saline-alkali soil and cultivated for one week, the organic matter content, hydrolyzable nitrogen, available phosphorus, and available potassium in the soil were significantly improved compared with the control group, and after planting Chinese cabbage, the germination rate and plant height of the Chinese cabbage in the soil of the embodiment were much higher than those of the Chinese cabbage in the control group. It can be seen that after the improver of the present application is applied to saline-alkali soil, the effective ingredients in the soil can be effectively increased, the improvement effect can be achieved, and the growth of plants can be effectively promoted.

[0053] Compared with Example 3, when the pH of the citric acid-sodium citrate solution in step S1 is changed, the improvement effect of the improver on the soil obtained in Example 6 tends to be lower than that in Example 3. It can be seen that when the pH of the citric acid-sodium citrate buffer solution in step S1 is within the range of 4.5-5.5, the improvement effect of the improver on the soil can be effectively guaranteed.

[0054] Compared with Example 3, when the pH of the citric acid-sodium citrate solution in step S1 is changed, the improvement effect of the improver obtained in Example 7-8 on the soil is increased compared with Example 3. However, with the increase of pH, the content of organic matter, hydrolyzable nitrogen, available phosphorus, and available potassium in the soil first increases and then decreases. It can be seen that when the pH value of the citric acid-sodium citrate solution in step S2 is 4-5, the improvement effect of the improver on the soil can be effectively guaranteed.

[0055] Compared with Example 7, when alanine is added in step S2, after the improver obtained in Example 9-11 is applied to saline-alkali soil, the pH of the saline-alkali soil decreases more than that of the control group, and the organic matter content, hydrolyzable nitrogen, available phosphorus, and available potassium in the soil are significantly improved compared with Example 7. It can be seen that the addition of alanine can effectively increase the activity of the complex enzyme molecules in the soil, thereby greatly enhancing the improvement effect of the improver on the soil.

[0056] Comparative Example 11-1 and Comparative Example 11-2, when an equal amount of bentonite or trehalose is used instead of alanine, the soil pH value of Comparative Example 11 is increased compared with Example 1, and the content of organic matter, hydrolyzable nitrogen, available phosphorus, and available potassium is reduced compared with Example 11. It can be seen that the coordinated use of the various components in the present application can effectively ensure the improvement effect of the modifier on the soil.

[0057] Compared with Example 3, when the composite enzyme lacks acid phosphatase and xylanase, the improvement effect of the improver obtained in Comparative Example 1-2 on the soil is lower than that in Example 3. It can be seen that the coordinated use of cellulase, protease, urease, xylanase and acid phosphatase can effectively improve the improvement effect of the improver on the soil.

[0058] Compared with Example 3, when wood vinegar is not added in step S2, or the amount of carrier added exceeds the limit of the present application, the improvement effect of the improver obtained in Comparative Examples 3-4 on the soil is reduced, indicating that the coordination between the raw materials has an important influence on the improvement effect of the improver.

[0059] Table 2 Content of various substances in saline-alkali soil after 60 days project pH Organic matter g / kg Hydrolyzable nitrogen mg / kg Available phosphorus mg / kg Available potassium mg / kg Example 1 8.67 21.12 78.45 22.53 163.52 Example 2 8.63 21.45 79.54 23.46 164.61 Example 3 8.55 21.62 81.65 23.92 165.58 Example 4 8.55 21.59 81.29 23.90 165.41 Example 5 8.55 21.53 81.21 23.91 165.35 Example 6 8.59 21.53 81.29 23.90 165.21 Example 7 8.51 21.74 82.34 23.99 168.76 Example 8 8.53 21.82 82.81 23.86 168.82 Example 9 8.38 23.42 93.31 25.75 185.54 Example 10 8.34 24.56 96.57 26.12 193.12 Embodiment 11 8.35 23.32 95.34 26.08 192.68 Comparative Example 11-1 8.45 21.02 85.35 24.32 178.62 Comparative Example 11-2 8.67 21.42 84.69 23.69 175.45 Control group 9.01 5.36 30.52 13.21 135.146 Comparative Example 1 8.89 17.54 56.15 20.12 154.38 Comparative Example 2 8.91 17.31 56.21 20.06 156.40 Comparative Example 3 8.86 16.42 54.32 20.18 152.75 Comparative Example 4 8.91 14.32 50.56 19.17 152.84 It can be seen from Table 2 that after the improver obtained in the embodiment of the present application was used in the soil for 60 days, the organic matter content, available phosphorus content and available potassium content in the soil decreased less than the data in Table 1. It can be seen that the improver obtained in the present application has better lasting performance in soil improvement.

[0060] The embodiments of this specific implementation method are all preferred embodiments of the present invention, and are not intended to limit the protection scope of the present invention. Therefore, any equivalent changes made based on the structure, shape, and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A saline-alkali land improver with added bio-enzyme preparation, characterized in that: The improver comprises the following raw materials in parts by weight: 10-20 parts of cellulase, 3-8 parts of protease, 1-5 parts of urease, 5-15 parts of xylanase, 4-10 parts of phosphatase, 3-9 parts of earthworm castings, 10-18 parts of stabilizer, 3-8 parts of wood vinegar and 10-20 parts of carrier.

2. The saline-alkali land improver with added biological enzyme preparation according to claim 1, characterized in that: The invention comprises 12-17 parts of cellulase, 4.5-6 parts of protease, 2-4 parts of urease, 8-12 parts of xylanase, 5-8 parts of phosphatase, 4-7 parts of earthworm castings, 12-16 parts of stabilizer, 4-6.5 parts of wood vinegar and 12-18 parts of carrier.

3. The saline-alkali land improver with added biological enzyme preparation according to claim 1 or 2, characterized in that: The phosphatase used is acid phosphatase.

4. The saline-alkali land improver with added biological enzyme preparation according to claim 1 or 2, characterized in that: The stabilizer is sodium polyacrylate or polyvinyl pyrrolidone.

5. The saline-alkali land improver with added biological enzyme preparation according to claim 1 or 2, characterized in that: The carrier consists of diatomaceous earth and chitosan in a weight ratio of 1:

1.

6. The saline-alkali land improver with added biological enzyme preparation according to claim 5, characterized in that: The particle size of the diatomaceous earth is 50-60 meshes, and the particle size of the chitosan is 80-100 meshes.

7. A method for preparing a saline-alkali land improver with added bio-enzyme preparation according to any one of claims 1 to 6, characterized in that: The method comprises the following preparation steps: S1, mixing cellulase, protease, urease, xylanase and phosphatase according to weight parts to obtain a composite enzyme, and then adding a buffer solution to prepare a composite enzyme solution; S2, after the carrier and the stabilizer are mixed, the complex enzyme solution obtained in step S1 is added, and then the buffer solution 2 is added to solidify for 5-6 hours at 23±1°C and a rotation speed of 200±10r / min, and then filtered, and then mixed with wood vinegar and earthworm castings and dried to obtain an improver.

8. The method for preparing a saline-alkali land improver with added biological enzyme preparation according to claim 7, characterized in that: The buffer solution 1 and the buffer solution 2 are both citric acid-sodium citrate solutions, the pH of the buffer solution 1 is 4.5-5.5, and the pH of the buffer solution 2 is 4-5.

9. The method for preparing a saline-alkali land improver with added bio-enzyme preparation according to claim 7, characterized in that: In the step S2, a buffer solution II and alanine are added and solidified for 5-6 hours at 23±1° C. and a rotation speed of 200±10 r / min, and then filtered, and then mixed with wood vinegar and earthworm castings and dried to obtain an improver.

10. The method for preparing a saline-alkali land improver with added biological enzyme preparation according to claim 9, characterized in that: The amount of alanine added is 1-1.5% of the weight of the complex enzyme.