Human brain glioma related fibroblast as well as acquisition method and application thereof
Through new isolation and culture methods, using collagenase P digestion and specific culture medium, the extraction success rate and proliferation rate of human brain glioma-related fibroblasts were successfully improved, and the limitations and instability of extracting and cultured cells in the prior art were solved.
CN119931928APending Publication Date: 2025-05-06BEIJING NEUROSURGICAL INST
0 Cites 0 Cited by
Patent Information
- Application Number
- CN202510063696.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-15
- Publication Date
- 2025-05-06
Smart Images
Figure CN119931928A_ABST
Abstract
The invention relates to a human brain glioma related fibroblast as well as an acquisition method and application thereof. The human brain glioma related fibroblast strain is named as G-CAF-1110, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.46060. The acquisition method of the human brain glioma related fibroblasts comprises a step of acquiring cells from brain glioma tissue fragments by adopting collagenase P digestive juice, a step of primary culture and a step of subculture, and a culture medium is a DMEM / F-12 culture solution and contains 5-12 vol% of human platelet lysate, 1.5-2.5 vol% of fetal calf serum and 0.8-1.2 vol% of mycillin. The cell can be used for screening medicines for treating human brain glioma and the like.
Need to check novelty before this filing date? Find Prior Art