Intelligent ultralow-temperature biological sample storage cabinet and use method

Abstract

The invention discloses an intelligent ultralow-temperature biological sample storage cabinet and a using method, the intelligent ultralow-temperature biological sample storage cabinet comprises a cabinet body and a sample tube, a storage assembly is installed in the cabinet body, a placing and taking-out assembly is installed in the cabinet body, and the placing and taking-out assembly is used for moving the sample tube into the storage assembly; the storage assembly comprises a rotary placement assembly and a driving assembly for driving the rotary placement assembly to rotate; by means of the well-designed storage assembly and the well-designed placing and taking-out assembly, sample tubes loaded with biological samples can be easily and safely stored in the cabinet body or accurately taken out from the deep position of the cabinet body, intelligent storage and taking of the sample tubes are achieved, and the working efficiency of experimenters during treatment of a large number of samples is greatly improved; manual intervention and outdoor operation are reduced, so that the risk of sample damage is effectively reduced. And meanwhile, the maximization of the sample storage amount is also realized, and a more convenient and efficient sample management solution is provided for biological experiments.

Description

Technical Field

[0001] The present invention relates to the technical field of biological sample storage, and in particular to an intelligent ultra-low temperature biological sample storage cabinet and a use method thereof. Background Art

[0002] Various biological samples such as tissues, blood, cells, organs, DNA, RNA, biological fluids, etc. generally need to be stored at low temperatures. Most of them are stored in an environment of -80°C and the storage containers are ultra-low temperature manual refrigerators.

[0003] In traditional ultra-low temperature manual refrigerators, storing or removing biological samples is all done manually, and there is a high probability of error during manual operation, and there is a risk of frostbite. At the same time, since the refrigerator door must be opened every time to store and retrieve biological samples, this process will bring in a large amount of water vapor, causing severe frost on the surface of the sample, affecting the identification of the sample.

[0004] As biological sample storage has received more and more attention, automated storage equipment has been introduced and gradually replaced ultra-low temperature manual refrigerators. However, the existing fully automatic ultra-low temperature biological sample storage equipment, for example, CN110589332A, an automated biological sample library, although equipped with a turntable rotation module, a basket lifting module, a shovel plate module, a tube picking module and a transfer module, has a relatively complex structure. The complex internal structure has led to a significant increase in the manufacturing cost of the equipment, and this has also increased the difficulty and cost of equipment maintenance to a certain extent. In addition, existing equipment usually adopts a compartmentalized storage design, but this not only reduces the actual available storage space, but also affects the storage efficiency of samples. In particular, when the sample demand is large or a large number of samples need to be stored for a long time, the low effective volume ratio is more prominent, which greatly limits the application scope and applicability of the equipment. Summary of the invention

[0005] The purpose of the present invention is to provide an intelligent ultra-low temperature biological sample storage cabinet and a method of use to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions: an intelligent ultra-low temperature biological sample storage cabinet, comprising a cabinet body and a sample tube, wherein a storage component is installed inside the cabinet body, and a placement and removal component is installed inside the cabinet body, wherein the placement and removal component is used to move the sample tube to the interior of the storage component; The storage component includes a rotating placement component and a driving component for driving the rotating placement component to rotate. The rotating placement component includes a rotating frame. A plurality of entry and exit slots are provided inside the rotating frame wall. A plurality of storage frames are installed on the inner side of the rotating frame, and the positions of the plurality of storage frames correspond to the positions of the entry and exit slots respectively. A push-out port is provided on one side of the storage frame.

[0007] Preferably, the driving assembly includes a support base, a driving motor is fixedly installed inside the support base, the output end of the driving motor is fixedly connected to the bottom of the rotating frame, a plurality of entry and exit slots are provided inside the rotating frame, a partition is fixedly installed inside the cabinet, the support base is fixedly installed on the top of the partition, circular through holes are provided on the top and both sides of the storage frame, and a plurality of rectangular groove groups are provided inside the rotating frame.

[0008] Preferably, the rectangular slot groups are arranged in a plurality of groups at intervals along the circumferential direction of the side wall of the rotating frame, each rectangular slot group includes a plurality of strip-shaped heat dissipation holes longitudinally arranged along the height direction of the rotating frame, and a plurality of storage frames are arranged from top to bottom on the inner wall of the rotating frame between any two rectangular slot groups, each of the storage frames is in a cube shape, and the storage frames are arranged corresponding to the inlet and outlet slots, and the driving motor drives the rotating frame to rotate, and the driving motor is provided with a first magnetic induction switch, and a magnetic induction sheet is provided in the middle of the lower outer wall of each storage frame located around the bottom of the rotating frame, and each of the magnetic induction sheets forms a unique position correspondence with the plurality of storage frames above the same column; A control box is fixedly installed at the bottom of the inner cavity of the cabinet, and a controller is installed in the control box. The controller is electrically connected to the drive motor and the first magnetic induction switch respectively. During the access process of the sample tube, after the target storage frame corresponding to the access of the sample tube is determined, the corresponding magnetic induction sheet is locked according to the position of the target storage frame, and the controller controls the drive motor to drive the rotating frame to rotate. At the same time, during the rotation process, the first magnetic induction switch senses the signal of each magnetic induction sheet in real time. When the first magnetic induction switch senses the corresponding magnetic induction sheet, the controller determines that it has reached the target storage frame and controls the drive motor to stop working.

[0009] Preferably, a switch button is installed on the front of the cabinet, a control display screen is installed on the front of the cabinet, an entry and exit frame is installed on the front of the cabinet, an entry and exit slot is opened on the top of the entry and exit frame, an intelligent sealing door is installed on the top of the entry and exit frame, a sealing slide groove is opened on one side of the entry and exit frame, a sealing slider is slidably installed inside the sealing slide groove, a connecting block is fixedly installed on one side of the sealing slider, a sample plate is fixedly installed on the top of the connecting block, a movable seat is movably installed on the top of the sample plate, a placement seat is clamped and installed on the top of the movable seat, a sample tube is placed on the top of the placement seat, a control box is fixedly installed on the bottom of the cabinet body cavity, and a programmed cooling instrument is fixedly installed on both sides of the cabinet body cavity.

[0010] Preferably, the placement and removal component includes an adjusting electric cylinder, a connecting plate is fixedly installed on the output end of the adjusting electric cylinder, a limiting slide is fixedly installed on the bottom of one end of the connecting plate, a moving frame is fixedly installed on one end of the limiting slide, a moving block is slidably installed inside the moving frame, two springs are fixedly connected to the top of the moving block, and the top of the spring is fixedly connected to the bottom of the inner cavity of the moving frame, a pushing electric cylinder is fixedly installed on one side of the moving block, and a pushing plate is fixedly installed on the output end of the pushing electric cylinder.

[0011] Preferably, a connecting rod 2 is fixedly installed on the top of the connecting plate away from the limiting slide plate, a connecting bending rod is fixedly installed on the top of the connecting rod 2, a connecting rod 1 is fixedly installed on the bottom of the connecting bending rod away from the end of the connecting rod 2, a moving frame 2 is fixedly installed on the bottom of the connecting rod 1, a moving block 2 is slidably installed inside the moving frame 2, two springs 2 are fixedly connected to the top of the moving block 2, and the top of the spring 2 is fixedly connected to the bottom of the inner cavity of the moving frame 2, a pushing electric cylinder 2 is fixedly installed on one side of the moving block 2, a pushing plate 2 is fixedly installed on the output end of the pushing electric cylinder 2, and the moving frame 2 and the pushing electric cylinder 2 are both located on the inner side of the rotating frame.

[0012] Preferably, a fixed connecting rod is fixedly installed on the bottom of the connecting plate away from the limiting slide plate, one side of the bottom of the fixed connecting rod is fixedly connected to the end of the sealing slider away from the connecting block, a vertical plate is fixedly installed inside the cabinet, a limiting slide groove is provided inside the vertical plate, the limiting slide plate is slidably installed on the inner side of the limiting slide groove, an elastic sealing belt is installed inside the sealing slide groove, and the elastic sealing belt is fixedly connected to the bottom of the sealing slider.

[0013] Preferably, an inverted T-shaped slide groove 1 is opened inside the sample plate, an inverted T-shaped slider is fixedly installed on the bottom of the movable seat, and the inverted T-shaped slider is slidably installed on the inner side of the sample plate, and an inverted T-shaped slide groove 2 is opened inside the rotating frame and the storage frame, and the specifications and dimensions of the inverted T-shaped slide groove 2 are compatible with the specifications and dimensions of the inverted T-shaped slider.

[0014] Preferably, an outer protective shell is fixedly installed on the outer side of the cabinet, observation windows are installed inside the outer protective shell and the cabinet, inspection doors are installed on both sides of the cabinet, heat dissipation ports are opened on both sides of the cabinet, programmed cooling devices are installed on both sides of the inner cavity of the cabinet, the programmed cooling device cools down the inside of the cabinet, the heat dissipation ports correspond to the heat dissipation points of the programmed cooling device, an RFID scanner is installed on the front of the cabinet, and a USB interface is installed on the front of the cabinet.

[0015] Preferably, the rotating frame wall is equipped with a plurality of temperature sensors, the controller is electrically connected to the program cooling device and the temperature sensors respectively, and the controller is used to perform temperature control on the program cooling device, including: Get the start control instruction of the program cooling device; The program cooling device is started according to the program cooling device start control instruction, and the response time of the program cooling device start control instruction is obtained; When the program cooling instrument cools down the sample tube inside the cabinet, the temperature change information of the temperature sensor is obtained in real time; Analyze the temperature change information of the temperature sensor to determine the temperature change characteristics inside the cabinet; Based on the temperature change characteristics inside the cabinet, the cooling temperature is predicted to obtain the cooling prediction information inside the cabinet; Determine the target analysis time according to the response time of the program cooling instrument start control instruction, analyze the cabinet temperature drop prediction information in combination with the target analysis time, determine whether the cabinet internal temperature reaches the cabinet internal temperature minimum value after the target analysis time, and obtain the prediction analysis result; When the predicted analysis result shows that the temperature inside the cabinet does not reach the minimum value of the temperature inside the cabinet after the target analysis time, the program cooling device continues to cool the inside of the cabinet; When the predicted analysis result shows that the temperature inside the cabinet reaches the minimum value of the temperature inside the cabinet after the target analysis time, a program cooling device pause control instruction is obtained to stop the program cooling device from cooling the inside of the cabinet (1).

[0016] A method for using an intelligent ultra-low temperature biological sample storage cabinet comprises the following steps: S1: Input information: Before placing and storing the sample tube, the RFID tag on the outside of the sample tube can be scanned by an RFID scanner, and the scanning result will be transmitted to the control box. The processor inside the control box can process the sample information of the sample tube and start the program cooling device to cool the inside of the cabinet so that the temperature inside the cabinet is suitable for storing the sample inside the sample tube; S2: Sample storage: First, place the sample tube on the top of the placement seat, then input instructions through the control display screen to start the adjustment electric cylinder inside the placement and removal component and retract the output end, so that the sample tube enters the interior of the cabinet. In addition, the drive motor can drive the rotating frame to rotate after it is started. By adjusting the electric cylinder and the drive motor, the sample plate can be aligned with one of the entry and exit slots and the storage frame. Then, the push electric cylinder 1 is started to embed the mobile seat carrying the placement seat and the sample tube into the storage frame, thereby completing the storage of the sample tube; S3: Sample removal: When the output end of the control-adjusting electric cylinder drives the sample plate to move to the front of the storage frame where the sample tube that needs to be removed is stored, the pushing electric cylinder 2 is started. The output end of the pushing electric cylinder 2 can push the pushing plate 2 to move, thereby pushing the moving seat carrying the placement seat and the sample tube to the top of the sample plate, and then the output end of the adjusting electric cylinder is started to extend, thereby removing the sample tube from the inside of the cabinet.

[0017] Compared with the prior art, the beneficial effects of the present invention are: through the carefully designed storage components and placement and removal components, the sample tubes loaded with biological samples can be easily and safely stored inside the cabinet, or accurately taken out from the depths of the cabinet, which not only realizes the intelligent storage and access of sample tubes, but also greatly improves the work efficiency of experimenters when handling a large number of samples, and effectively reduces the risk of sample damage by reducing manual intervention and outdoor operations. At the same time, it also realizes the maximization of sample storage capacity, providing a more convenient and efficient sample management solution for biological experiments; The storage components are set up to ensure that the sample tubes are stably stored in the cabinet and are easy to locate quickly, so as to ensure that the sample tubes will not interfere with each other during storage and will not be damaged by vibration or temperature changes. This design not only improves the storage density, but also optimizes the space utilization, so that more sample tubes can be accommodated inside the cabinet. The rotating frame can rotate around the center point, so that different storage frames can be aligned with the access ports of the sample plate in turn, which not only improves the flexibility of sample storage, but also enables the experimenter to quickly select and access specific sample tubes as needed. The setting of multiple storage frames further increases the sample storage capacity, meeting the high demand for sample storage in large-scale biological experiments; The placement and retrieval component uses a highly automated mechanical structure to achieve fast and accurate placement and retrieval of sample tubes. Experimenters only need to follow simple operating instructions to allow the placement and retrieval component to automatically complete the transportation of sample tubes. During this process, there is no need for manual operation to directly open the cabinet door, thus avoiding the risk of contamination caused by outdoor operations and ensuring the purity and integrity of the samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the three-dimensional appearance structure of the present invention.

[0019] Figure 2 For the present invention Figure 1 Enlarged structural diagram at A in the middle.

[0020] Figure 3 It is a schematic diagram of the cross-sectional structure of the cabinet of the present invention.

[0021] Figure 4 It is a schematic diagram of the three-dimensional structure of the storage component and the placement and removal component of the present invention.

[0022] Figure 5 It is a schematic diagram of the three-dimensional structure of the rotating placement component of the present invention.

[0023] Figure 6 It is a schematic diagram of the three-dimensional structure of the placement and removal component of the present invention.

[0024] Figure 7 This is a schematic diagram of the three-dimensional structure of the placement and removal component of the present invention from another perspective.

[0025] Figure 8 A flowchart diagram is used for the present invention.

[0026] In the figure: 1, cabinet; 2, outer protective shell; 3, observation window; 4, switch button; 5, RFID scanner; 6, USB interface; 7, control display screen; 8, entry and exit frame; 9, maintenance door; 10, heat dissipation port; 11, sample tube; 12, placement seat; 13, moving seat; 14, sample plate; 15, inverted T-shaped slide groove 1; 16, inverted T-shaped slider; 17, connecting block; 18, sealing slide groove; 19, rotating frame; 20, partition; 21, support seat; 22, control box; 23, program cooling instrument; 24, connecting bending rod; 25, rectangular groove group; 26, storage frame; 2 7. Circular through hole; 28. Inlet and outlet slots; 29. ​​Intelligent sealing door; 30. Moving frame 1; 31. Vertical plate; 32. Limiting slide groove; 33. Inverted T-shaped slide groove 2; 34. Connecting rod 1; 35. Push-out port; 36. Adjusting electric cylinder; 37. Connecting rod 2; 38. Driving motor; 39. Spring 2; 40. Moving frame 2; 41. Moving block 2; 42. Pushing electric cylinder 2; 43. Pushing plate 2; 44. Connecting plate; 45. Limiting slide plate; 46. Sealing slider; 47. Fixed connecting rod; 48. Spring 1; 49. Moving block 1; 50. Pushing electric cylinder 1; 51. Pushing plate 1. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] See also Figure 1-Figure 8The present invention provides a technical solution: an intelligent ultra-low temperature biological sample storage cabinet, comprising a cabinet body 1 and a sample tube 11, a storage component is installed inside the cabinet body 1, and the storage component includes a support seat 21, a driving motor 38 is fixedly installed inside the support seat 21, a rotating frame 19 is fixedly installed at the output end of the driving motor 38, a plurality of inlet and outlet slots 28 are provided inside the rotating frame 19, a plurality of storage frames 26 are fixedly installed inside the rotating frame 19, and a push-out port 35 is provided on one side of the storage frames 26, a partition 20 is fixedly installed inside the cabinet body 1, the support seat 21 is fixedly installed on the top of the partition 20, a circular through hole 27 is provided on the top and both sides of the storage frame 26, a plurality of rectangular slot groups 25 are provided inside the rotating frame 19, and the positions of the plurality of storage frames 26 correspond to the positions of the plurality of inlet and outlet slots 28 respectively.

[0029] See also Figure 4 and Figure 5 In order to accurately reach the position of the storage frame 26 corresponding to the storage and access process of the sample tube 11, the positioning component provided by the present invention includes a magnetic induction sheet, a first magnetic induction switch, a controller and a drive motor 38. Specifically, a plurality of rectangular slot groups 25 are arranged at intervals along the circumferential direction of the side wall of the rotating frame 19, and each rectangular slot group 25 includes a plurality of strip heat dissipation holes arranged longitudinally along the height direction of the rotating frame 19. A plurality of storage frames 26 are arranged from top to bottom on the inner wall of the rotating frame 19 between any two rectangular slot groups 25. Each storage frame 26 is in a cube shape, and each storage frame 26 is arranged corresponding to the inlet and outlet slots 28. This structural arrangement can improve the heat dissipation, and can ensure that both sides of each storage frame 26 have strip heat dissipation holes for heat dissipation, and ensure that the temperature in the storage frame 26 is the internal temperature of the cabinet 1 to the greatest extent, avoiding local heat accumulation, thereby ensuring that the sample tube 11 is in a low-temperature storage state, and ensuring the storage conditions of the samples on the sample tube 11.

[0030] During the access process of the sample, the driving motor 38 drives the rotating frame 19 to rotate. The driving motor 38 is provided with a first magnetic induction switch. A magnetic induction sheet is provided at the middle of the lower outer wall of each storage frame 26 located around the bottom of the rotating frame 19. Each magnetic induction sheet forms a unique position correspondence with the multiple storage frames 26 above the same column. A control box 22 is fixedly installed at the bottom of the inner cavity of the cabinet 1, and a controller is installed in the control box 22. The controller is electrically connected to the drive motor 38 and the first magnetic induction switch respectively. During the access process of the sample tube 11, after determining the target storage frame 26 corresponding to the access of the sample tube 11, the corresponding magnetic induction sheet is locked according to the position of the target storage frame 26. The controller controls the drive motor 38 to drive the rotating frame 19 to rotate. At the same time, during the rotation process, the first magnetic induction switch senses the signals of each magnetic induction sheet in real time. When the first magnetic induction switch senses the corresponding magnetic induction sheet, the controller determines that it has reached the target storage frame 26 and controls the drive motor 38 to stop working.

[0031] The principle and effect of the above technical solution are as follows: by forming a corresponding matching relationship between each group of storage frames 26 and a magnetic sensing sheet, in the process of accessing the sample tube 11, it is necessary to determine the storage frame 26 corresponding to the access. If the storage frames 26 are in the same column, they can correspond to the same access position relationship, that is, the rotating frame 19 is rotated to the same position by driving the motor 38. During the rotation process, the signals of each magnetic sensing sheet are sensed in real time by the first magnetic induction switch, and each magnetic sensing sheet is assigned a different label. Then, a corresponding relationship is formed between the magnetic induction sheet and the storage frame 26 in each column, and the labels are stored in the controller. In this way, when the first magnetic induction switch senses the corresponding magnetic induction sheet, the controller can determine that the corresponding storage frame 26 has been transferred to the required position, that is, the sample tube 11 can be accessed. The setting of this solution can ensure that in the process of accessing the sample tube 11, the required storage frame 26 can be quickly and intelligently rotated to the target position, thereby improving work efficiency and intelligence.

[0032] A placement and removal component is installed inside the cabinet 1, a switch button 4 is installed on the front of the cabinet 1, a control display screen 7 is installed on the front of the cabinet 1, an entry and exit frame 8 is installed on the front of the cabinet 1, an entry and exit slot is provided on the top of the entry and exit frame 8, an intelligent sealing door 29 is installed on the top of the entry and exit frame 8, a sealing slide groove 18 is provided on one side of the entry and exit frame 8, a sealing slider 46 is slidably installed inside the sealing slide groove 18, a connecting block 17 is fixedly installed on one side of the sealing slider 46, a sample plate 14 is fixedly installed on the top of the connecting block 17, a movable seat 13 is movably installed on the top of the sample plate 14, a placement seat 12 is snap-fitted on the top of the movable seat 13, and a sample tube 11 is placed on the top of the placement seat 12.

[0033] The placement and removal component includes an adjusting electric cylinder 36, a connecting plate 44 is fixedly installed on the output end of the adjusting electric cylinder 36, a limiting slide 45 is fixedly installed on the bottom of one end of the connecting plate 44, a moving frame 30 is fixedly installed on one end of the limiting slide 45, a moving block 49 is slidably installed inside the moving frame 30, two springs 48 are fixedly connected to the top of the moving block 49, and the top of the spring 48 is fixedly connected to the bottom of the inner cavity of the moving frame 30, a pushing electric cylinder 50 is fixedly installed on one side of the moving block 49, a pushing plate 51 is fixedly installed on the output end of the pushing electric cylinder 50, a connecting rod 237 is fixedly installed on the top of the connecting plate 44 away from the limiting slide 45, a connecting bending rod 24 is fixedly installed on the top of the connecting rod 237, a connecting rod 34 is fixedly installed on the bottom of the connecting bending rod 24 away from the end of the connecting rod 237, and a moving frame 40 is fixedly installed on the bottom of the connecting rod 34 A moving block 2 41 is slidably installed inside the moving frame 2 40, and two springs 2 39 are fixedly connected to the top of the moving block 2 41, and the top of the spring 2 39 is fixedly connected to the bottom of the inner cavity of the moving frame 2 40, and a pushing electric cylinder 2 42 is fixedly installed on one side of the moving block 41, and a pushing plate 2 43 is fixedly installed on the output end of the pushing electric cylinder 2 42. The moving frame 2 40 and the pushing electric cylinder 2 42 are both located on the inner side of the rotating frame 19, and a fixed connecting rod 47 is fixedly installed at the bottom of the connecting plate 44 away from the end of the limiting slide plate 45, and one side of the bottom of the fixed connecting rod 47 is fixedly connected to the end of the sealing slider 46 away from the connecting block 17, and a vertical plate 31 is fixedly installed inside the cabinet 1, and a limiting slide groove 32 is provided inside the vertical plate 31, and the limiting slide plate 45 is slidably installed on the inner side of the limiting slide groove 32, and an elastic sealing belt is installed inside the sealing slide groove 18, and the elastic sealing belt is fixedly connected to the bottom of the sealing slider 46.

[0034] Both sides of the inner cavity of the cabinet 1 are fixedly installed with a program cooling device 23, which cools the inside of the cabinet 1. The rotating frame 19 is equipped with a plurality of temperature sensors, which can sense the internal temperature of the cabinet 1. The controller is electrically connected to the program cooling device 23 and the temperature sensor, respectively, and the controller is used to control the temperature of the program cooling device 23, including: Obtaining a start control instruction of the program cooling device 23; The program cooling device 23 is started according to the program cooling device 23 start control instruction, and the response time of the program cooling device 23 start control instruction is obtained; When the program cooling device 23 cools the sample tube 11 inside the cabinet 1, the temperature change information of the temperature sensor is obtained in real time; Analyze the temperature change information of the temperature sensor to determine the temperature change characteristics inside the cabinet 1; Based on the temperature variation characteristics inside the cabinet 1, a cooling temperature prediction is performed to obtain the cooling prediction information inside the cabinet 1; Determine the target analysis time according to the response time of the control instruction of the program cooling device 23, analyze the temperature reduction prediction information in the cabinet 1 in combination with the target analysis time, determine whether the internal temperature of the cabinet 1 reaches the minimum value of the internal temperature of the cabinet 1 after the target analysis time, and obtain the prediction analysis result; When the predicted analysis result shows that the temperature inside the cabinet 1 does not reach the minimum temperature inside the cabinet 1 after the target analysis time, the programmed temperature reduction device 23 continues to reduce the temperature inside the cabinet 1; When the predicted analysis result shows that the internal temperature of the cabinet 1 reaches the minimum internal temperature of the cabinet 1 after the target analysis time, a pause control instruction of the program cooling device 23 is obtained to stop the program cooling device 23 from cooling the inside of the cabinet 1 .

[0035] The minimum value of the temperature inside the cabinet 1 is determined based on the temperature adaptability range of the refrigerated storage of the samples in the cabinet 1 .

[0036] The technical effect of the above content is: the intelligent temperature control of the cabinet 1 is realized through the controller and the temperature sensor, so that the program cooling device 23 can better cool the storage environment of the sample tube 11 in the cabinet 1, and at the same time, it can also prevent the program cooling device 23 from cooling the temperature in the cabinet 1 too much, thereby affecting the refrigeration storage effect of the sample tube 11. In addition, in the intelligent temperature control process, by obtaining the response time of the program cooling device 23 to start the control instruction, it is clear how much time delay there is in the response of the control instruction, so that when the program cooling device 23 is stopped to cool the environment in the cabinet 1, the time delay of the control instruction is fully considered, and it is avoided that the program cooling device 23 cools the cabinet 1 by the program cooling device 23 exceeding the minimum value of the internal temperature of the cabinet 1 due to the suspension of the control instruction of the program cooling device 23, thereby improving the accuracy of the program cooling device 23 cooling the environment in the cabinet 1, thereby improving the intelligent performance of the temperature environment control in the cabinet 1, and providing guarantee for the effective storage of the sample tube 11.

[0037] The working principle of the above technical solution is as follows: after the external power supply is connected, the device is turned on by the switch button 4, and the program cooling device 23 is turned on to cool the inside of the cabinet 1, so that the temperature inside the cabinet 1 is suitable for preserving the sample inside the sample tube 11. When the sample tube 11 needs to be stored, the sample tube 11 can be placed on the top of the placement seat 12, and then the placement seat 12 is placed inside the movable seat 13, and then the movable seat 13 is placed on the top of the sample plate 14, and then the command is input through the control display screen 7, and the command will be transmitted to the processor inside the control box 22, and then the processor will transmit the data to the controller, and the controller controls the adjustment electric cylinder 36 and the drive motor 38 to start, and after the adjustment electric cylinder 36 is started, the output end retracts, thereby bringing The movable connecting plate 44 moves upward, and the sealing slider 46 is pulled to move upward inside the sealing slide groove 18 through the fixed connecting rod 47. When the sample plate 14 approaches the intelligent sealing door 29, the intelligent sealing door 29 will automatically open, so that the sample plate 14 carrying the moving seat 13, the placement seat 12 and the sample tube 11 enter the interior of the cabinet 1. After entering, the intelligent sealing door 29 will automatically close to ensure the sealing and safety of the interior of the cabinet 1. In addition, when the connecting plate 44 moves upward, the moving frame 30 can be driven to move upward through the connecting plate 44. When the moving frame 30 moves upward, the moving block 49 and the pushing electric cylinder 50 will first be in a stationary state under the action of the elastic force of the spring 48, and then the moving frame 30 will pull the moving block 13 through the spring 48. 49 and the push electric cylinder 50 move upward, and make the position of the push plate 51 correspond to the position height of the moving seat 13. In addition, after the drive motor 38 is started, it can drive the rotating frame 19 to rotate, so that one column of the storage frames 26 corresponds to the position of the sample plate 14. By adjusting the cooperation of the electric cylinder 36 and the drive motor 38, the sample plate 14 can correspond to the position of one of the inlet and outlet slots 28 and the storage frame 26. Then, the push electric cylinder 50 is started, and the output end of the push electric cylinder 50 can push the push plate 51 to move, so that the moving seat 13 is pushed away from the top of the sample plate 14 by the push plate 51, and the moving seat 13 carrying the placement seat 12 and the sample tube 11 is pushed into the storage frame 26, thereby completing the sample The storage of the tube 11, in addition, when sampling is required, when the output end of the adjusting electric cylinder 36 drives the sample plate 14 to move to the front of the storage frame 26 where the movable seat 13, the placement seat 12 and the sample tube 11 that need to be taken out are stored, the connecting plate 44 can also drive the movable frame 40 to move through the connecting rod 2 37, the connecting bending rod 24 and the connecting rod 1 34, so that the pushing electric cylinder 2 42 and the pushing plate 2 43 are aligned with the ejection port 35 on the back of the storage frame 26, and then the pushing electric cylinder 2 42 is started, and the output end of the pushing electric cylinder 2 42 can push the pushing plate 2 43 to move, so that the movable seat 13 carrying the placement seat 12 and the sample tube 11 are pushed to the top of the sample plate 14 through the pushing plate 2 43, and then the output end of the adjusting electric cylinder 36 is started to extend,Thus, the sample plate 14 carrying the moving seat 13, the placement seat 12 and the sample tube 11 is brought close to the intelligent sealing door 29. The intelligent sealing door 29 opens automatically, so that the sample plate 14 carrying the moving seat 13, the placement seat 12 and the sample tube 11 can be removed from the inside of the cabinet 1, which is convenient for the experimenter to perform sampling operations and can intelligently store and access the sample tube 11, greatly improving the efficiency of the experimenter in storing and accessing the sample tube 11, and there is no need to manually open the cabinet door, avoiding outdoor operations and damage to the sample, and the rotation of the rotating frame 19 and the multiple storage frames 26 greatly increase the sample storage capacity, making the storage and access of biological samples more flexible, and greatly improving the efficiency of storing and accessing the sample tube 11.

[0038] In another embodiment, Figure 1-Figure 8 As shown, an inverted T-shaped slide groove 15 is provided inside the sample plate 14, an inverted T-shaped slider 16 is fixedly installed on the bottom of the movable seat 13, and the inverted T-shaped slider 16 is slidably installed on the inner side of the sample plate 14, and an inverted T-shaped slide groove 23 is provided inside the rotating frame 19 and the storage frame 26, and the specifications and dimensions of the inverted T-shaped slide groove 23 are compatible with the specifications and dimensions of the inverted T-shaped slider 16.

[0039] The inverted T-shaped slide groove 15 and the inverted T-shaped slider 16 can ensure the stability of the moving seat 13 moving on the top of the sample plate 14, and the inverted T-shaped slide groove 2 33 can ensure the stability of the moving seat 13 moving inside the storage frame 26 and the rotating frame 19, thereby ensuring the stability of storing and removing the sample tube 11.

[0040] In another embodiment, Figure 1-Figure 8 As shown, an outer protective shell 2 is fixedly installed on the outer side of the cabinet 1, and observation windows 3 are installed inside the outer protective shell 2 and the cabinet 1. Inspection doors 9 are installed on both sides of the cabinet 1. Heat dissipation ports 10 are opened on both sides of the cabinet 1, and the heat dissipation ports 10 correspond to the heat dissipation places of the programmed cooling device 23. An RFID scanner 5 is installed on the front of the cabinet 1, and a USB interface 6 is installed on the front of the cabinet 1.

[0041] The cabinet 1 is provided to facilitate the staff to observe the process of storing and taking out the sample tubes 11, thereby ensuring the normal use of the device. The inspection door 9 is provided to facilitate the inspection of the equipment inside the cabinet 1. The heat dissipation port 10 is provided to discharge the heat generated when the program cooling device 23 is running.

[0042] The method for using the intelligent ultra-low temperature biological sample storage cabinet includes the following steps: S1: Input information: Before placing and storing the sample tube 11, the RFID tag on the outside of the sample tube 11 can be scanned by the RFID scanner 5, and the scanning result will be transmitted to the control box 22. In addition to the controller, the control box 22 also includes a processor, an information storage device and a PC board. The processor inside the control box 22 can process the sample information of the sample tube 11, including the sample type, the expected storage location and the expected storage time, which are transmitted to the control display screen 7 for display after being processed by the processor inside the control box 22, and the information of the sample tube 11 is stored in the information storage device inside the control box 22, and the program cooling device 23 is turned on to cool the inside of the cabinet 1, so that the temperature inside the cabinet 1 is suitable for storing the sample inside the sample tube 11; S2: Sample storage: When the sample tube 11 needs to be stored, the sample tube 11 can be placed on the top of the placement seat 12, and then the placement seat 12 is placed inside the moving seat 13, and then the moving seat 13 is placed on the top of the sample plate 14, and then the command is input through the control display screen 7, and the command will be transmitted to the processor inside the control box 22, and then the processor will transmit the data to the controller, and the controller controls the adjustment electric cylinder 36 and the drive motor 38 to start. After the adjustment electric cylinder 36 is started, the output end retracts, thereby driving the connecting plate 44 to move upward, and the seal is pulled by the fixed connecting rod 47. The sealing slider 46 moves upward inside the sealing slot 18. When the sample plate 14 approaches the intelligent sealing door 29, the intelligent sealing door 29 will automatically open, so that the sample plate 14 carrying the moving seat 13, the placement seat 12 and the sample tube 11 enter the interior of the cabinet 1. After entering, the intelligent sealing door 29 will automatically close to ensure the sealing and safety of the interior of the cabinet 1. In addition, when the connecting plate 44 moves upward, the moving frame 30 can be driven to move upward through the connecting plate 44. When the moving frame 30 moves upward, the moving block 49 and the pushing electric cylinder 50 will first move upward under the action of the elastic force of the spring 48. The moving frame 30 is in a stationary state, and then the moving block 49 and the pushing electric cylinder 50 are pulled upward by the spring 48, and the position of the pushing plate 51 corresponds to the position height of the moving seat 13. In addition, after the driving motor 38 is started, the rotating frame 19 can be driven to rotate, so that one column of the storage frames 26 corresponds to the position of the sample plate 14. By adjusting the cooperation of the electric cylinder 36 and the driving motor 38, the sample plate 14 can correspond to the position of one of the in-and-out slots 28 and the storage frame 26, and then the pushing electric cylinder 50 is started, and the output end of the pushing electric cylinder 50 can push The push plate 1 51 is moved, so that the moving seat 13 is pushed away from the top of the sample plate 14 by the push plate 1 51, and the moving seat 13 carrying the placement seat 12 and the sample tube 11 is pushed into the storage frame 26, so as to complete the storage of the sample tube 11. The stability of the moving seat 13 moving on the top of the sample plate 14 can be ensured by the inverted T-shaped slide groove 1 15 and the inverted T-shaped slider 16, and the stability of the moving seat 13 moving inside the storage frame 26 and the rotating frame 19 can be ensured by the inverted T-shaped slide groove 2 33, so as to ensure the stability of the storage and removal of the sample tube 11. S3: Sample removal: When the output end of the control-adjusting electric cylinder 36 drives the sample plate 14 to move to the front of the storage frame 26 where the movable seat 13, the placement seat 12 and the sample tube 11 to be removed are stored, the connecting plate 44 can also drive the movable frame 2 40 to move through the connecting rod 2 37, the connecting bending rod 24 and the connecting rod 1 34, so that the pushing electric cylinder 2 42 and the pushing plate 2 43 are aligned with the ejection port 35 on the back of the storage frame 26, and then the pushing electric cylinder 2 42 is started. The output end of the pushing electric cylinder 2 42 can push the pushing plate 2 43 to move, so that the movable seat 13 carrying the placement seat 12 and the sample tube 11 are pushed to the top of the sample plate 14 through the pushing plate 2 43, and then the output end of the adjusting electric cylinder 36 is started to extend, so that the sample plate 14 carrying the movable seat 13, the placement seat 12 and the sample tube 11 are close to the intelligent sealing door 29. The intelligent sealing door 29 opens automatically, allowing the sample plate 14 carrying the movable seat 13, the placement seat 12 and the sample tube 11 to escape from the interior of the cabinet 1, making it convenient for the experimenter to perform sampling operations and being able to intelligently access the sample tube 11, greatly improving the efficiency of the experimenter in accessing the sample tube 11. There is no need to manually open the cabinet door, thus avoiding outdoor operations and damage to the samples. The rotation of the rotating frame 19 and the multiple storage frames 26 greatly increase the sample storage capacity, making the access to biological samples more flexible and greatly improving the efficiency of accessing the sample tube 11.

[0043] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An intelligent ultra-low temperature biological sample storage cabinet, comprising a cabinet body (1) and a sample tube (11), characterized in that: A storage component is installed inside the cabinet (1), and a placement and removal component is installed inside the cabinet (1), wherein the placement and removal component is used to move the sample tube (11) into the interior of the storage component; The storage assembly comprises a rotating placement assembly and a driving assembly for driving the rotating placement assembly to rotate. The rotating placement assembly comprises a rotating frame (19). A plurality of entry and exit slots (28) are provided inside a frame wall of the rotating frame (19). A plurality of storage frames (26) are installed inside the rotating frame (19). The positions of the plurality of storage frames (26) respectively correspond to the positions of the entry and exit slots (28). A push-out port (35) is provided on one side of each of the storage frames (26).

2. The intelligent ultra-low temperature biological sample storage cabinet according to claim 1 is characterized in that: The driving assembly comprises a support base (21), a driving motor (38) is fixedly installed inside the support base (21), an output end of the driving motor (38) is fixedly connected to the bottom of the rotating frame (19), a partition (20) is fixedly installed inside the cabinet (1), the support base (21) is fixedly installed on the top of the partition (20), the top and both sides of the storage frame (26) are provided with circular through holes (27), and a plurality of rectangular groove groups (25) are provided inside the rotating frame (19).

3. The intelligent ultra-low temperature biological sample storage cabinet according to claim 2 is characterized in that: A plurality of rectangular slot groups (25) are arranged at intervals along the circumferential direction of the side wall of the rotating frame (19), each rectangular slot group (25) comprises a plurality of strip-shaped heat dissipation holes arranged longitudinally along the height direction of the rotating frame (19), a plurality of storage frames (26) are arranged from top to bottom on the inner wall of the rotating frame (19) between any two rectangular slot groups (25), each storage frame (26) is in a cube shape, the storage frame (26) is arranged corresponding to the inlet and outlet slots (28), the driving motor (38) drives the rotating frame (19) to rotate, the driving motor (38) is provided with a first magnetic induction switch, a magnetic induction sheet is provided at the middle of the lower outer wall of each storage frame (26) located around the bottom of the rotating frame (19), and each magnetic induction sheet forms a unique position corresponding relationship with the plurality of storage frames (26) above the same column; A control box (22) is fixedly mounted at the bottom of the inner cavity of the cabinet (1), and a controller is mounted in the control box (22). The controller is electrically connected to the drive motor (38) and the first magnetic induction switch respectively. During the access process of the sample tube (11), after the target storage frame (26) corresponding to the access of the sample tube (11) is determined, the corresponding magnetic induction sheet is locked according to the position of the target storage frame (26). The controller controls the drive motor (38) to drive the rotating frame (19) to rotate. At the same time, during the rotation process, the first magnetic induction switch senses the signal of each magnetic induction sheet in real time. When the first magnetic induction switch senses the corresponding magnetic induction sheet, the controller determines that the target storage frame (26) has been reached, and controls the drive motor (38) to stop working.

4. The intelligent ultra-low temperature biological sample storage cabinet according to claim 3 is characterized in that: A switch button (4) is installed on the front of the cabinet (1), a control display screen (7) is installed on the front of the cabinet (1), an entry and exit frame (8) is installed on the front of the cabinet (1), an entry and exit slot is opened on the top of the entry and exit frame (8), an intelligent sealing door (29) is installed on the top of the entry and exit frame (8), a sealing slide groove (18) is opened on one side of the entry and exit frame (8), a sealing slider (46) is slidably installed inside the sealing slide groove (18), a connecting block (17) is fixedly installed on one side of the sealing slider (46), a sample plate (14) is fixedly installed on the top of the connecting block (17), a movable seat (13) is movably installed on the top of the sample plate (14), a placement seat (12) is clamped and installed on the top of the movable seat (13), and a sample tube (11) is placed on the top of the placement seat (12).

5. The intelligent ultra-low temperature biological sample storage cabinet according to claim 4 is characterized in that: The placement and removal component comprises an adjusting electric cylinder (36), a connecting plate (44) is fixedly mounted on the output end of the adjusting electric cylinder (36), a limiting slide plate (45) is fixedly mounted on the bottom of one end of the connecting plate (44), a moving frame (30) is fixedly mounted on one end of the limiting slide plate (45), a moving block (49) is slidably mounted inside the moving frame (30), two springs (48) are fixedly connected to the top of the moving block (49), and the top of the spring (48) is fixedly connected to the bottom of the inner cavity of the moving frame (30), a pushing electric cylinder (50) is fixedly mounted on one side of the moving block (49), and a pushing plate (51) is fixedly mounted on the output end of the pushing electric cylinder (50).

6. The intelligent ultra-low temperature biological sample storage cabinet according to claim 5 is characterized in that: A second connecting rod (37) is fixedly mounted on the top of the connecting plate (44) at one end away from the limiting slide plate (45); a connecting bending rod (24) is fixedly mounted on the top of the second connecting rod (37); a connecting rod (34) is fixedly mounted on the bottom of the connecting bending rod (24) at one end away from the second connecting rod (37); a second moving frame (40) is fixedly mounted on the bottom of the first connecting rod (34); a second moving block (41) is slidably mounted inside the second moving frame (40); two second springs (39) are fixedly connected to the top of the second moving block (41); and the top of the second spring (39) is fixedly connected to the bottom of the inner cavity of the second moving frame (40); a second pushing electric cylinder (42) is fixedly mounted on one side of the second moving block (41); a second pushing plate (43) is fixedly mounted on the output end of the second pushing electric cylinder (42); and the second moving frame (40) and the second pushing electric cylinder (42) are both located on the inner side of the rotating frame (19).

7. The intelligent ultra-low temperature biological sample storage cabinet according to claim 4 is characterized in that: A fixed connecting rod (47) is fixedly installed at the bottom of one end of the connecting plate (44) away from the limiting slide plate (45), and one side of the bottom of the fixed connecting rod (47) is fixedly connected to one end of the sealing slide block (46) away from the connecting block (17). A vertical plate (31) is fixedly installed inside the cabinet (1), and a limiting slide groove (32) is provided inside the vertical plate (31). The limiting slide plate (45) is slidably installed on the inner side of the limiting slide groove (32). An elastic sealing belt is installed inside the sealing slide groove (18), and the elastic sealing belt is fixedly connected to the bottom of the sealing slide block (46).

8. The intelligent ultra-low temperature biological sample storage cabinet according to claim 4 is characterized in that: An inverted T-shaped slide groove (15) is provided inside the sample plate (14), an inverted T-shaped slider (16) is fixedly installed on the bottom of the movable seat (13), and the inverted T-shaped slider (16) is slidably installed on the inner side of the sample plate (14). An inverted T-shaped slide groove (33) is provided inside the rotating frame (19) and the storage frame (26), and the specifications and dimensions of the inverted T-shaped slide groove (33) are compatible with the specifications and dimensions of the inverted T-shaped slider (16).

9. The intelligent ultra-low temperature biological sample storage cabinet according to claim 6 is characterized in that: An outer protective shell (2) is fixedly mounted on the outer side of the cabinet (1); observation windows (3) are mounted inside the outer protective shell (2) and the cabinet (1); inspection doors (9) are mounted on both sides of the cabinet (1); heat dissipation ports (10) are opened on both sides of the cabinet (1); programmed cooling devices (23) are mounted on both sides of the inner cavity of the cabinet (1); the programmed cooling devices (23) cool the inside of the cabinet (1); the heat dissipation ports (10) correspond to the heat dissipation locations of the programmed cooling devices (23); an RFID scanner (5) is mounted on the front side of the cabinet (1); and a USB interface (6) is mounted on the front side of the cabinet (1).

10. The intelligent ultra-low temperature biological sample storage cabinet according to claim 9, characterized in that: The rotating frame (19) is provided with a plurality of temperature sensors mounted on its wall. The controller is electrically connected to the programmed cooling device (23) and the temperature sensors respectively. The controller is used to control the temperature of the programmed cooling device (23), including: Obtaining a start control instruction of a program cooling device (23); Starting the programmed cooling device (23) according to a start-up control instruction of the programmed cooling device (23), and obtaining a response time of the start-up control instruction of the programmed cooling device (23); When the programmed temperature-lowering device (23) cools the sample tube (11) inside the cabinet (1), temperature change information of the temperature sensor is obtained in real time; Analyze the temperature change information of the temperature sensor to determine the temperature change characteristics inside the cabinet (1); Predicting the cooling temperature based on the temperature variation characteristics inside the cabinet (1) to obtain the cooling prediction information inside the cabinet (1); Determine the target analysis time according to the response time of the control command started by the program cooling device (23), analyze the temperature drop prediction information in the cabinet (1) in combination with the target analysis time, determine whether the temperature inside the cabinet (1) reaches the minimum value of the temperature inside the cabinet (1) after the target analysis time, and obtain the prediction analysis result; When the predicted analysis result shows that the temperature inside the cabinet (1) does not reach the minimum temperature inside the cabinet (1) after the target analysis time, the programmed temperature reduction device (23) continues to reduce the temperature inside the cabinet (1); When the predicted analysis result shows that the temperature inside the cabinet (1) reaches the minimum temperature inside the cabinet (1) after the target analysis time, a pause control instruction of the program cooling device (23) is obtained, and the program cooling device (23) is stopped to cool the inside of the cabinet (1).

11. A method for using an intelligent ultra-low temperature biological sample storage cabinet, characterized in that: The following steps are involved: S1: Inputting information: Before placing and storing the sample tube (11), the RFID tag on the outside of the sample tube (11) can be scanned by the RFID scanner (5), and the scanning result can be transmitted to the control box (22). The processor inside the control box (22) can process the sample information of the sample tube (11), and start the program cooling device (23) to cool the inside of the cabinet (1), so that the temperature inside the cabinet (1) is suitable for storing the sample inside the sample tube (11); S2: Sample storage: First, the sample tube (11) is placed on the top of the placement seat (12), and then a command is input through the control display screen (7) to start the adjustment electric cylinder (36) inside the placement and removal component and the output end is retracted, so that the sample tube (11) enters the interior of the cabinet (1). In addition, the driving motor (38) can drive the rotating frame (19) to rotate after it is started. By cooperating with the adjustment electric cylinder (36) and the driving motor (38), the sample plate (14) can be aligned with the position of one of the entry and exit slots (28) and the storage frame (26). Then, the pushing electric cylinder (50) is started to embed the movable seat (13) carrying the placement seat (12) and the sample tube (11) into the interior of the storage frame (26), thereby completing the storage of the sample tube (11); S3: Sample removal: When the output end of the control-adjusting electric cylinder (36) drives the sample plate (14) to move to the front of the storage frame (26) where the sample tube (11) to be removed is stored, the pushing electric cylinder (42) is started. The output end of the pushing electric cylinder (42) can push the pushing plate (43) to move, thereby pushing the moving seat (13) carrying the placement seat (12) and the sample tube (11) to the top of the sample plate (14), and then the output end of the adjusting electric cylinder (36) is started to extend, thereby removing the sample tube (11) from the inside of the cabinet (1).

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