Soothing and repairing composition and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a soothing and repairing composition and application thereof. The soothing and repairing composition comprises the following components in percentage by weight: 2%-5% of osmanthus fragrans essence cell sap, 3%-5% of rhamnose, 0.5%-2% of tetrahydromethylpyrimidine carboxylic acid and the balance of water and / or common additives in cosmetics. According to the soothing and repairing composition provided by the invention, plant extracts (osmanthus fragrans essence cell sap) and repairing components are scientifically matched and combined, and after the specific raw materials are compounded, the soothing and repairing composition has a remarkable effect on sensitive skin and has a synergistic effect. The data result of the embodiment of the invention shows that the composition can promote absorption and metabolism, relieve skin allergic inflammation and accelerate skin barrier repair.

Description

Technical Field

[0001] The present application belongs to the field of cosmetic technology, and specifically relates to a soothing and repairing composition and its application. Background Art

[0002] Sensitive skin is a physiological reaction symptom rather than a disease caused by the stimulation of allergens. Most women and men in developed countries believe that they have this skin symptom and that their skin is more susceptible to the external environment or facial products than ordinary people and overreacts. Sensory sensations include: facial tightness, tingling, burning, pain, persistent numbness and itching, accompanied by skin redness and inflammation, etc.

[0003] There are many factors that cause skin allergies: Climate influence: The stimulation of the hot and cold temperatures in spring and the various pollens, microbial dust in nature blown to the face by the wind cause spring dermatitis; in summer, ultraviolet rays promote oxidative stress, strengthen the inflammatory process, and cause light sensitivity and stinging; in autumn, the weather is dry and the temperature drops. The skin metabolism has not fully adapted to the climate change, which reduces the secretion of sebum and sweat glands and damages the barrier; the cold climate in winter prevents our skin from secreting enough sebum, resulting in dry skin, facial skin begins to peel, and then accompanied by erythema. Stressful emotions: The fast pace of life leads to anxiety, irregular rest, etc. Eating habits: sweets, chocolate, fat, soda acidic foods, additives, tobacco, alcohol, spicy, etc. Cosmetics: extreme means, fragrances, preservatives, some chemicals, etc. Others include topical preparations containing antibiotics, hormones, etc., environmental pollution, air quality, automobile exhaust, domestic water quality, toxic particles, heavy metals, PM2.5, etc. The factors that cause sensitive skin are complex and diverse. Long-term immersion in numerous allergens can lead to damaged skin barrier function, inflammatory state of the epidermis, abnormalities of the skin vascular system, skin sensory nervous system, and skin microbiome. The superposition of these factors can cause the skin to gradually become fragile and sensitive.

[0004] For external hair treatments for sensitive skin, most people currently choose mild cosmetics that do not contain fragrances or preservatives. When allergies are severe, external preparations containing antibiotics and hormones are used. Cosmetics are daily skin care products that moisturize and soothe the skin to reduce allergies. Although the use of anti-allergic drugs such as antibiotics and hormones to treat skin allergies is fast, they are easy to accumulate in the blood, causing skin function disorders and mutations of factors such as immunoglobulin E. When encountering allergens again, the skin loses its normal response function, so it fails to truly repair the skin, and the skin is prone to repeated allergies, thus forming a dependence on drugs. Therefore, it is urgent to develop a skin care product that can truly repair the skin. Summary of the invention

[0005] The purpose of the present invention is to solve the deficiencies of the prior art and provide a soothing and repairing composition and its application, which specifically adopts the following technical solutions: In a first aspect, the present invention provides a soothing and repairing composition, which comprises the following components in parts by weight: Osmanthus extract cell fluid 2%-5%, rhamnose 3%-5%, ectoine 0.5%-2%, the remainder is water and / or additives commonly used in cosmetics.

[0006] The present invention scientifically combines the plant extract (osmanthus essence cell fluid) and repairing ingredients. After compounding with these specific raw materials, it has a significant effect on sensitive skin and has a synergistic effect.

[0007] The rhamnose used in the present invention is obtained by extracting hesperidin from natural sweet orange, degrading it through yeast fermentation, filtering, concentrating, crystallizing and drying it. It is a natural, mild and highly effective skin soothing agent. Through in vitro skin reconstruction experiments and in vivo clinical experiments on young and elderly donors, it was found that rhamnose not only has a positive effect on the epidermis and dermis, but also can improve the activity of papillary fibroblasts, promote the growth of keratinocyte growth factor (KGF) and vascular endothelial growth factor (VEGF), increase the thickness of the skin epidermis, and thus make the skin firmer and more elastic. In addition, rhamnose can bind to cytokines secreted by keratinocytes (interleukins, TNF-α, etc.), and block the generation of inflammatory factor signals. Tetrahydromethylpyrimidine carboxylic acid is derived from Halomonas halophilic bacteria. Elongata), so it is also called "halophilic bacteria extract". Under extreme conditions of high salt, high temperature and high ultraviolet radiation, ectoine can protect halophilic bacteria from harm. At the same time, ectoine can build a strong protective layer of water molecules in the skin, which can strengthen and restore cell function, stabilize the skin barrier, and restore and regulate water content. It can help the skin lock in moisture and maintain a good growth environment for cells. At the same time, it also helps the skin barrier to recover, keep the skin healthy and moist, and has a good repair effect on the skin barrier.

[0008] As a further preferred embodiment, it comprises the following components in parts by weight: Osmanthus extract cell fluid 3%, rhamnose 5%, ectoine 1%, the balance is water and / or additives commonly used in cosmetics.

[0009] As a further preferred embodiment, the above-mentioned osmanthus extract cell fluid is prepared by the following method: The osmanthus flower cell fluid is extracted by vacuum microwave distillation technology, and then the osmanthus flower extract cell fluid is prepared by constant temperature distillation and concentration.

[0010] As a further preferred embodiment, the volatile characteristic components contained in the above-mentioned osmanthus extract cell fluid include β-ionone and linalool. These two are qualitative indicators and quality control indicators of this product. The rest include hexadecanoic acid, methyl eicosatrienoate, 3-(3-hydroxybutyl)-2,2,4-trimethyl-2-cyclohexene-1-one, γ-caprylic acid lactone, α-ionone, dihydro-β-ionone, geraniol, nerol, lauric acid, palmitic acid, stearic acid, ethyl caprylate, decahydronaphthalene, p-hydroxyphenylethanol, safrole, isomenthone, elemi resin and other ingredients, which are not qualitative components.

[0011] It should be noted that the osmanthus extract cell fluid is mainly water and volatile components of osmanthus, and β-ionone and linalool have the highest content in the volatile components, which are qualitative indicators of the product, and the other indicators are non-qualitative components. In addition, the non-volatile components contained in the cell fluid of the osmanthus extract include trace soluble proteins and non-volatile oils. The non-volatile oils are mainly split-cyclic-cyclic terpenes. Due to their trace amounts, non-volatility and solubility in this product, they are not used as qualitative indicators.

[0012] In a second aspect, the present invention provides the use of the soothing and repairing composition in the preparation of a skin care product with barrier repairing effect.

[0013] In a third aspect, the present invention provides the use of the soothing and repairing composition in the preparation of skin care products with anti-inflammatory effects. In a fourth aspect, the present invention provides the use of the soothing and repairing composition in the preparation of a skin care product having barrier repair and anti-inflammatory effects.

[0014] In a fifth aspect, the present invention provides a skin care product, comprising: A cosmetic base solvent and the above soothing and repairing composition.

[0015] As a further preferred embodiment, the skin care product includes any one of a facial cream, a lotion, a gel, a serum, a serum water, and a facial mask.

[0016] The beneficial effects of the present invention are as follows: the present invention provides a soothing and repairing composition, which is scientifically combined with the plant extract (osmanthus extract cell fluid) and the repairing ingredients, and after compounding with this specific raw material, has a significant effect on sensitive skin and has a synergistic effect. The data results of the embodiments of the present invention show that the composition can promote absorption and metabolism, soothe skin allergic inflammation, and accelerate the repair of the skin barrier. DETAILED DESCRIPTION

[0017] The following will be combined with the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0018] Example 1 Osmanthus fragrans is sequentially subjected to vacuum microwave water gravity technology and vacuum microwave distillation technology to extract osmanthus fragrans cell fluid, and then the osmanthus fragrans extract cell fluid is obtained by constant temperature distillation and concentration. The specific process is as follows: From 2015 to 2021, from mid-September to early October each year, the applicant picked fresh osmanthus flowers from the organic osmanthus base planted in Fuyan Village, Xiaoxiba Town, Jiangyou City, Sichuan Province, and within 30 minutes after the fresh osmanthus flowers were separated from the osmanthus trees, the field heat was eliminated to 6°C by vacuum precooling, and then vacuum-packed in nylon vacuum bags with less than 1 pinhole per square centimeter, and then sent to a -36°C quick freezer for quick freezing for 3 hours, and then transferred to a -10°C freezer for storage. After the frozen ice osmanthus flowers were naturally thawed, they were sent to a vacuum microwave extraction device to extract osmanthus cell water, and the collected osmanthus cell water was concentrated by vacuum distillation concentration equipment to obtain osmanthus extract cell fluid.

[0019] Example 2 Detection of components in the cell fluid of osmanthus extract The volatile oil content of the osmanthus extract cell fluid was detected by GC-MS. The two volatile active components with the highest content were linalool (5~10) and β-ionone (4~8), so they were determined to be characteristic components (the results are shown in Table 1-3).

[0020] Table 1 Table 2 Table 3 Example 3 Evaluation experiment on the soothing effect of the soothing repair composition (2%-5% osmanthus extract cell fluid, 3%-5% rhamnose, 0.5%-2% ectoine) 1. Evaluation method: Hyaluronidase inhibition experiment operation instructions 2. Experimental purpose and principle: Relevant literature shows that hyaluronic acid is widely present in the skin and other tissues, and the relationship between hyaluronic acid and the skin is very close. Macromolecular hyaluronic acid can inhibit inflammatory reactions, thereby alleviating the discomfort caused by skin inflammation. Macromolecular hyaluronic acid will be degraded under the action of hyaluronidase, resulting in a decrease in the content of macromolecular hyaluronic acid. The decomposition produces a degradation product, N-acetylglucosamine. Determining the content of N-acetylglucosamine in the reaction system can indirectly reflect the activity of hyaluronidase.

[0021] 3. Relevance of test methods: This method is an in vitro method and is suitable for testing soothing products. Relevant literature shows that the trend of the in vivo method has a certain correlation with this in vitro method. Through experimental design, the N-acetylglucosamine content in the reaction system is measured to determine the inhibition rate of the test sample on hyaluronidase. If the inhibition rate of the test sample is better (higher) than that of the blank control group and the statistical difference P value is <0.05, it can be considered that the test sample has a certain soothing effect.

[0022] Table 4 Test items 4. Experimental formula and control group Table 5 5. Experimental methods 5.1 Weighing samples; 5.2 Pre-treat the test sample and then pre-treat it to make a mixed solution; 5.3 Add the reactants and mix well, then place in a water bath at 37°C; 5.4 Add pH adjuster, place in boiling water for 10 minutes, and cool to room temperature; 5.5 Measure the OD value by machine test, calculate the hyaluronidase activity inhibition rate and statistical difference P value; 6. Experimental results Table 6 Test results statistics 7. Analysis and Conclusion The inhibition rate of the experimental samples is better than (higher than) the inhibition rate of the blank control group and the statistical difference P value is <0.05, indicating that the difference is significant. The experimental samples have a soothing effect. The inhibition rate of product group 4 is better than that of product group 3, which is better than that of product group 2, and which is better than that of product group 1. The inhibition rates of product group 3, product group 2, and product group 1 increase significantly, while the increase between product group 4 and product group 3 is gentle, and the inhibition rates are not much different.

[0023] Example 4 Evaluation experiment on the antipruritic effect of the slow-repairing composition (2%-5% osmanthus extract cell fluid, 3%-5% rhamnose, 0.5%-2% ectoine) 1. Experimental purpose and principle: After a certain concentration of 4-aminopyridine solution is injected subcutaneously, mice will have an emergency licking reaction (the mice will repeatedly turn their heads and lick the backs on both sides, i.e., the licking reaction). Inject 0.01% 4-aminopyridine solution in advance, and then observe the number of licking times of the mice within 10 minutes (the number of lickings is counted as one if the mice lick the body continuously until a brief pause), perform data statistics, and analyze whether it has an antipruritic effect based on the results.

[0024] 3. Experimental materials: 4-Aminopyridine Compound dexamethasone acetate cream A gel containing 2%-5% osmanthus extract cell fluid, 3%-5% rhamnose, and 0.5%-2% ectoine Ultrapure water 4. Experimental methods: 4.1. Preparation of 0.01% 4-aminopyridine solution: Accurately weigh 10 mg of 4-aminopyridine and place it in a volumetric flask. Add ultrapure water to completely dissolve it, and make up to 100 mL. Mix well to obtain the solution.

[0025] 4.2. The preparation process of the gel containing 2%-5% osmanthus extract cell fluid, 3%-5% rhamnose, and 0.5%-2% ectoine is as follows: 60% water, 3g glycerol, 0.5g carbomer, 0.5g hexylene glycol, and 0.4g p-hydroxyacetophenone are added into a beaker, heated to 75°C with stirring in a water bath, stirred and homogenized for 1-3 minutes until the raw materials are completely dissolved, cooled to below 45°C, 3g osmanthus extract cell fluid, 5g rhamnose, and 1g ectoine are added, stirred until completely dissolved, and then 0.5g triethanolamine is added and stirred to mix evenly to obtain 100g of gel.

[0026] 4.3. Adaptive feeding of mice: SPF KM mice were fed in an environment with a temperature of (25±2)℃ and a relative humidity of (75±10)% for 3 days.

[0027] 4.4. Grouping and treatment of mice: 16 mice were randomly divided into four groups according to their body weight: blank group, model group, positive group (compound dexamethasone acetate cream 0.075%), and drug administration group (gel containing 2%-5% osmanthus extract cell fluid, 3%-5% rhamnose, and 0.5%-2% ectoine), with 4 mice in each group, half male and half female. Use an electric shaver to cut off 2cm × 3cm of fur on the back of the mice, expose the skin, and then feed adaptively for three days.

[0028] 4.5. The blank group was smeared with ultrapure water on the back, the positive group was smeared with compound dexamethasone acetate cream, the drug-treated group was smeared with the gel prepared in step 4.2, and the model group was not treated. After 30 minutes, except for the blank group, the other groups were subcutaneously injected with ultrapure water, and 0.05 ml of 0.01% 4-aminopyridine solution was subcutaneously injected per 10 g of body weight. The number of body tadpoles of the mice within 10 minutes was immediately observed.

[0029] 5. Experimental results statistics 5.1. Data statistical analysis method: SPSS20.0 software was used for data statistical analysis. The results were expressed as mean ± standard deviation (mean ± SD). One-way analysis of variance was used to compare the number of body licking of mice in different treatment groups. The mean values ​​between groups were compared pairwise. LSD test was used for normal analysis and homogeneous variance; Dunnet's T3 test was used for non-normal analysis or unequal variance. p < 0.05 indicated statistically significant differences.

[0030] 5.2 Experimental Results Table 7 Study results of the antipruritic effect of the soothing and repairing composition Note: Compared with the blank group, * p<0.05, ** p<0.01 Compared with the model group, a p<0.05, aa p<0.01 Compared with the positive group, b p<0.05, bb p<0.01 The experimental results show that the number of licking of the model group mice after modeling is significantly different from that of the blank group, indicating that the modeling is successful. There is no significant difference in the data between the positive group and the model group, indicating that the selected positive drug (compound dexamethasone acetate cream) has no obvious antipruritic effect on 4-aminopyridine, but has a certain antipruritic trend. The data of the drug-treated group and the model group are significantly different, indicating that the soothing component (containing 2%-5% of osmanthus extract cell fluid, 3%-5% of rhamnose, and 0.5%-2% of ectomethylpyrimidine carboxylic acid) has a very obvious antipruritic effect on 4-aminopyridine-induced itching. The data of the drug-treated group and the positive group are significantly different, indicating that the soothing component (containing 2%-5% of osmanthus extract cell fluid, 3%-5% of rhamnose, and 0.5%-2% of ectomethylpyrimidine carboxylic acid) has a significant antipruritic effect on 4-aminopyridine-induced itching due to compound dexamethasone acetate cream.

[0031] Therefore, from the experimental process, it can be seen that 30 minutes after the mice were administered the drug, 4-aminopyridine was subcutaneously injected. The mice in the positive drug group (compound dexamethasone acetate cream) immediately showed a licking reaction, while the mice in the drug-administered group (gel containing 2%-5% osmanthus extract cell fluid, 3%-5% rhamnose, and 0.5%-2% ectoine) showed a licking reaction 26 seconds later, indicating that the antipruritic effect of the soothing component (gel containing 2%-5% osmanthus extract cell fluid, 3%-5% rhamnose, and 0.5%-2% ectoine) is rapid and better than compound dexamethasone acetate cream.

[0032] The embodiments of the present application are described above. Specific examples are used herein to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the core idea of ​​the present application, but the present application is not limited to the above-mentioned specific implementation methods. The above-mentioned specific implementation methods are merely illustrative and not restrictive. Under the guidance of the present application, ordinary technicians in this field can also make many forms without departing from the scope of protection of the purpose of the present application and the claims, all of which are within the protection of the present application.

Claims

1. A soothing and repairing composition, characterized in that: It includes the following components by weight percentage: Osmanthus extract cell fluid 2%-5%, rhamnose 3%-5%, ectoine 0.5%-2%, the remainder is water and / or additives commonly used in cosmetics.

2. The soothing and repairing composition according to claim 1, characterized in that: It includes the following components by weight percentage: Osmanthus extract cell fluid 3%, rhamnose 5%, ectoine 1%, the remainder is water and / or additives commonly used in cosmetics.

3. The soothing and repairing composition according to claim 2, characterized in that: The volatile characteristic components contained in the osmanthus extract cell fluid include beta-ionone and linalool.

4. The soothing and repairing composition according to claim 3, characterized in that: The osmanthus extract cell fluid also includes water.

5. The soothing and repairing composition according to any one of claims 1 to 2, characterized in that: The osmanthus extract cell fluid is prepared by the following method: The osmanthus extract cell fluid is obtained by sequentially subjecting the osmanthus to vacuum microwave extraction technology and vacuum concentration technology.

6. Use of the soothing and repairing composition according to any one of claims 1 to 5 in the preparation of a skin care product with barrier repairing effect.

7. Use of the soothing and repairing composition according to any one of claims 1 to 5 in the preparation of skin care products with anti-inflammatory effects.

8. Use of the soothing and repairing composition according to any one of claims 1 to 5 in the preparation of a skin care product with barrier repair and anti-inflammatory effects.

9. A skin care product, characterized in that: Include: Cosmetic base solvents; And the soothing repair composition according to any one of claims 1 to 5.

10. The skin care product according to claim 9, characterized in that: The skin care products include any one of facial cream, lotion, gel, essence, essence water and facial mask.

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