Traditional Chinese medicine hair growth lotion containing hair follicle stem cell exosome and having hair growth promoting effect and preparation method of traditional Chinese medicine hair growth lotion

Abstract

The invention relates to traditional Chinese medicine hair restorer containing hair follicle stem cell exosomes and having a hair growth promoting effect. The traditional Chinese medicine hair restorer comprises a traditional Chinese medicine aqueous extract and a traditional Chinese medicine alcohol extract, in addition, a surfactant, a solvent, a humectant and a bacteriostatic agent are added; the traditional Chinese medicine aqueous extract is prepared by extracting four traditional Chinese medicines selected from herba ecliptae, radix angelicae sinensis, flos carthami, radix astragali seu hedysari, poria cocos, rhizoma dioscoreae and fructus lycii; the traditional Chinese medicine alcohol extract is prepared by extracting six traditional Chinese medicines selected from glossy privet fruit, prepared rehmannia root, radix rehmanniae recen, salvia miltiorrhiza, ligusticum wallichii, radix paeoniae alba, cacumen biotae and polygonum multiflorum; the traditional Chinese medicine hair growth lotion containing the hair follicle stem cell exosome and having the hair growth promoting effect has the advantages of improving scalp microcirculation, stimulating hair follicle growth, improving the activity of hair follicle cells and promoting hair growth.

Description

Technical Field

[0001] The invention relates to a traditional Chinese medicine hair growth water containing hair follicle stem cell exosomes and having a hair growth promoting effect and a preparation method thereof. Background Art

[0002] As the aging population intensifies, the problem of hair loss becomes more prominent. According to statistics, more than 200 million people in China suffer from hair loss every year, which brings great troubles to people's lives, work and psychology.

[0003] The main mechanism of hair loss is that various factors have a negative effect on hair follicles, such as shortened hair growth period, prolonged catagen or resting period, hair follicle atrophy and degeneration, etc., which induces the change of hair follicle growth cycle, causing hair to fall out prematurely or hair to grow slowly or even unable to grow normally, which is visually manifested as a decrease in hair volume. This phenomenon of abnormal hair loss, which leads to sparse hair or receding hairline and seriously affects the patient's appearance, is called non-physiological hair loss, also known as pathological hair loss.

[0004] In ancient medical books, hair loss has many names such as "hair moth hair loss", "hair moth ringworm", "oil wind" and so on. There are many causes of hair loss, and the pathogenesis is complex, which belongs to "a mixture of deficiency and excess, deficiency in the root and excess in the superficial". The causes of hair loss are blood stasis, dampness and blood heat, which are all excess evils, but the ultimate cause of hair loss is insufficient nutrition and loss of nourishment. Therefore, in clinical treatment, it is necessary to "replenish the deficiency and remove the excess evil" and treat according to syndrome differentiation.

[0005] The Chinese herbal medicine Eclipta sinensis nourishes the liver and kidneys, improves eyesight and blackens hair, and can activate tyrosinase. Angelica sinensis inhibits the "5α-reductase" that causes hair loss, nourishes blood and promotes blood circulation. It contains a variety of hydrocarbon substances and vitamin B substances, which provide rich nutrition for hair follicles. Safflower has the effect of promoting blood circulation and removing blood stasis, and has the effect of promoting hair growth. Poria has the effects of promoting water and dampness, strengthening the spleen, and calming the mind. It has a more significant effect on seborrheic alopecia caused by excessive dampness in the body. Ligustrum lucidum has the effects of nourishing the liver and kidneys, cooling blood and stopping bleeding, and can promote blood circulation in the scalp and improve the nutritional supply of the scalp. Rehmannia root has the effects of clearing heat and cooling blood, nourishing yin and promoting body fluid. Rehmannia root has the effects of nourishing blood and nourishing yin, replenishing essence and filling marrow, effectively improving the scalp environment, promoting hair growth, and improving hair loss. Platycladus orientalis has the effects of nourishing the kidney and qi, cooling blood and stopping bleeding, and promoting hair growth and blackening hair. It is used to treat baldness, and long-term use can make hair dense and prevent hair loss. Salvia miltiorrhiza has the effects of nourishing qi and strengthening the body, anti-oxidation, anti-aging, and promoting hair growth and nourishing hair. Chuanxiong has the effect of promoting blood circulation and qi, and promoting blood circulation in the scalp. Astragalus has the effects of nourishing hair, improving hair quality, and maintaining endocrine balance. Its active ingredients can promote blood circulation in the scalp and improve the growth rate and quality of hair. Chinese yam has the effects of nourishing qi and yin, tonifying the spleen, lungs, and kidneys, and stopping leukorrhea. It can effectively improve blood circulation, improve the scalp environment, and promote hair growth. Lycium barbarum has the effects of nourishing the liver and kidneys, benefiting essence and improving eyesight. Lycium barbarum is rich in various nutrients, such as vitamin C, vitamin E, β-carotene, etc., which help to strengthen hair, improve hair elasticity, and reduce breakage and shedding. Polygonum multiflorum has the effects of promoting yang and yin, and blackening hair. White peony root has the effects of nourishing blood and regulating menstruation, restraining yin and stopping sweating, softening the liver and relieving pain, and calming liver yang. White peony root contains total glycosides of white peony, which can effectively improve alopecia areata and relieve hair loss. Minoxidil and finasteride are the only two drugs approved for the treatment of hair loss in China. Among them, the systemic exposure of finasteride leads to potential side effects, which hinders its wider use. Minoxidil is an OTC drug for external use, mainly in the form of tinctures, liniments, foams and gels, and can be used for seborrheic alopecia in both men and women. Its mechanism of action is still unclear, but it is generally believed that it stimulates hair follicle growth by forming minoxidil sulfate through sulfotransferase present in the root of hair follicles, and possible side effects include allergic dermatitis and itching.

[0006] In vitro studies have shown that the effect of stem cells on promoting blood vessel and hair follicle growth is closely related to the secretion of these nutritional factors. Nutritional factors can promote the formation of blood vessels around hair follicles during hair growth, increase the size of hair papilla cells and hair follicles, and thus accelerate hair growth. It has been clinically confirmed that minoxidil is used to treat hair growth in men and women with AGA and is also related to its expansion of blood vessels in hair follicles. Therefore, in addition to directly activating hair growth signals, the regulatory effect of stem cells on hair growth can also support and maintain hair growth by secreting various nutritional factors.

[0007] Exosomes are rich in information transfer substances such as DNA, RNA, and various proteins derived from mother cells. They play an important role in signal transmission between cells and are also used to culture cells. Studies have shown that exosomes contain a variety of growth factors and cytokines such as vascular endothelial growth factor (VEGF), stem cell factor (SCF), EGF, etc., and they mediate epithelial-mesenchymal interactions during organ development and can activate the Wnt / β-catenin signaling pathway. The expression of these growth factors and signaling molecules are all related to the inducing ability of hair papilla cells. Therefore, exosomes may promote the treatment of hair loss.

[0008] The chemical drugs currently used in the clinical treatment of hair loss have different degrees of side effects and the disadvantage of easy recurrence after discontinuation of the drugs. People have gradually begun to turn their attention to the research of natural drugs. Traditional Chinese medicine treats hair loss from a holistic perspective, and treats it based on syndrome differentiation. The drugs used have few toxic side effects, significant efficacy, and are easily accepted by patients. Therefore, patients with hair loss are more inclined to choose traditional Chinese medicine treatment methods to treat this disease. However, traditional Chinese medicine oral administration has the disadvantages of large dosage, inconvenient to carry and use, and long oral treatment cycle, which is not easily accepted by young people today.

[0009] Therefore, there is an urgent need in the art to develop a reliable, effective, convenient, refreshing, easily absorbed, low-irritation traditional Chinese medicine hair growth-promoting water containing exosomes. Summary of the invention

[0010] The present invention mainly solves the technical problems existing in the prior art, thereby providing a traditional Chinese medicine hair growth water which can improve scalp microcirculation, stimulate hair follicle growth, increase the activity of hair follicle cells and promote hair growth.

[0011] The above technical problems of the present invention are mainly solved by the following technical solutions:

[0012] The Chinese medicine hair-growth water containing hair follicle stem cell exosomes and having the function of promoting hair growth of the present invention comprises a Chinese medicine water extract and a Chinese medicine alcohol extract; and a surfactant, a solvent, a moisturizer and an antibacterial agent are further added;

[0013] The Chinese medicine water extract is made from four samples of Eclipta prostrata, Angelica sinensis, Carthamus tinctorius, Astragalus membranaceus, Poria cocos, Dioscorea opposita, and Lycium barbarum.

[0014] The Chinese medicine alcohol extract is made from six kinds of Chinese medicine selected from the group consisting of Ligustrum lucidum fruit, Rehmannia root, Radix Rehmanniae glutinosae, Salvia miltiorrhiza, Rhizoma Chuanxiong, Paeonia lactiflora, Platycladus orientalis leaves and Polygonum multiflorum.

[0015] Furthermore, the surfactant is selected from one or more of poloxamer, Tween 80, Span, and 1,3-butanediol; the solvent is selected from one or more of pure water, glycerol, and ethanol; the moisturizer is selected from one or more of panthenol and castor oil; the antibacterial agent is selected from one or more of phenoxyethanol and propylene glycol.

[0016] Furthermore, the Chinese medicinal materials used in the aqueous extract of Chinese medicinal materials are Eclipta prostrata, Angelica sinensis, Carthamus tinctorius, and Poria cocos, with the mass of each medicinal material being 10 g; the Chinese medicinal materials used in the alcohol extract of Chinese medicinal materials are Fructus Ligustri Lucidi, Rehmannia glutinosa, Radix Rehmanniae glutinosae, Salvia miltiorrhiza, Rhizoma Chuanxiong, and Platycladus orientalis, with the mass of each medicinal material being 10 g; the surfactant accounts for 12%; the solvent, moisturizer, and antibacterial agent account for 56%, 1.5%, and 0.5%, respectively.

[0017] Furthermore, the Chinese medicinal materials used in the aqueous extract of Chinese medicinal materials are Eclipta prostrata, Angelica sinensis, Carthamus tinctorius, and Astragalus membranaceus, and the mass of each medicinal material is 10g; the Chinese medicinal materials used in the alcohol extract of Chinese medicinal materials are Fructus Ligustri Lucidi, Rehmannia glutinosa, Radix Rehmanniae glutinosae, Salvia miltiorrhiza, Rhizoma Chuanxiong, and Polygonum multiflorum, and the mass of each medicinal material is 10g; the surfactant accounts for 12%; the solvent, moisturizer and antibacterial agent account for 56%, 1.5% and 0.5% respectively.

[0018] Furthermore, the Chinese medicinal materials used in the aqueous extract of Chinese medicinal materials are Eclipta prostrata, Angelica sinensis, Poria cocos, and Dioscorea opposita, with the mass of each medicinal material being 10 g; the Chinese medicinal materials used in the alcohol extract of Chinese medicinal materials are Fructus Ligustri Lucidi, Rehmannia glutinosa, Radix Rehmanniae glutinosae, Salvia miltiorrhiza, Rhizoma Chuanxiong, and Platycladus orientalis, with the mass of each medicinal material being 10 g; the surfactant accounts for 12%; the solvent, moisturizer, and antibacterial agent account for 56%, 1.5%, and 0.5%, respectively.

[0019] Furthermore, the Chinese medicinal materials used in the aqueous extract of Chinese medicinal materials are Eclipta prostrata, Carthamus tinctorius, Poria cocos, and Astragalus membranaceus, and the mass of each medicinal material is 10g; the Chinese medicinal materials used in the alcohol extract of Chinese medicinal materials are Fructus Ligustri Lucidi, Rehmannia glutinosa, Radix Rehmanniae glutinosae, Salvia miltiorrhiza, Rhizoma Chuanxiong, and Polygonum multiflorum, and the mass of each medicinal material is 10g; the surfactant accounts for 12%; the solvent, moisturizer and antibacterial agent account for 56%, 1.5% and 0.5% respectively.

[0020] Furthermore, the Chinese medicines used in the aqueous extract of Chinese medicine are Angelica sinensis, safflower, Poria cocos, and Lycium barbarum, with the mass of each medicinal material being 10g; the Chinese medicines used in the alcohol extract of Chinese medicine are Ligustrum lucidum, Rehmannia root, Rehmannia root, Ligusticum chuanxiong, Platycladus orientalis, and Polygonum multiflorum, with the mass of each medicinal material being 10g; the surfactant accounts for 12%; the solvent, moisturizer, and antibacterial agent account for 56%, 1.5%, and 0.5%, respectively.

[0021] Furthermore, the Chinese medicines used in the aqueous extract of Chinese medicine are Angelica sinensis, safflower, Poria cocos, and Lycium barbarum, with the mass of each medicinal material being 10g; the Chinese medicines used in the alcohol extract of Chinese medicine are Ligustrum lucidum, Rehmannia root, Rehmannia root, Ligusticum chuanxiong, Platycladus orientalis, and Polygonum multiflorum, with the mass of each medicinal material being 10g; the surfactant accounts for 12%; the solvent, moisturizer, and antibacterial agent account for 56%, 1.5%, and 0.5%, respectively.

[0022] A method for preparing a traditional Chinese medicine hair-growth water containing hair follicle stem cell exosomes and having a hair-growth-promoting effect, comprising the following steps:

[0023] ① Take 4 samples of Eclipta prostrata, Angelica sinensis, Carthamus tinctorius, Astragalus membranaceus, Poria cocos, Dioscorea opposita, and Lycium barbarum, each with a mass of 10 g, add 10 times the amount of pure water for extraction, and obtain a Chinese medicine water extract (extract 20 mg / ml, calculated based on the amount of crude drug);

[0024] ② Take 6 samples of Ligustrum lucidum, Rehmannia root, Radix Rehmanniae, Salvia miltiorrhiza, Rhizoma Chuanxiong, Paeonia lactiflora, Platycladus orientalis, and Polygonum multiflorum, each with a weight of 10g, add 10 times the amount of 95% ethanol for extraction, and obtain the Chinese medicine alcohol extract (extract 20mg / ml, calculated based on the amount of crude drug);

[0025] ③ Mix the Chinese herbal medicine water extract and the Chinese herbal medicine alcohol extract in a volume ratio of 4:6 to obtain a Chinese herbal medicine mixed extract;

[0026] ④ Taking the total weight of the Chinese medicine hair growth liquid as 100%, take 30% of the Chinese medicine mixed extract, add 12% of the surfactant, stir and mix well to obtain phase A;

[0027] ⑤ Take 56% of the solvent and 1.5% of the moisturizer and mix them at a temperature of 80°C-85°C, mix them with the above phase A, and stir and mix them at a speed of 200rpm / min-250rpm / min. During the mixing process, add 0.5% of the antibacterial agent and mix them thoroughly;

[0028] ⑥Take the purified exosomes and mix them with the prepared Chinese medicine hair growth water to make the exosome concentration reach 50μg / ml.

[0029] The beneficial effects of the present invention are:

[0030] The traditional Chinese medicine hair growth water prepared by the invention can improve scalp microcirculation, stimulate hair follicle growth, and increase the activity of hair follicle cells, thereby achieving the effect of promoting hair growth. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0032] Figure 1 This is a comparison diagram of the effects before and after the use of the present invention. DETAILED DESCRIPTION

[0033] The preferred embodiments of the present invention are described in detail below in conjunction with the accompanying drawings so that the advantages and features of the present invention can be more easily understood by those skilled in the art, thereby making a clearer and more definite definition of the protection scope of the present invention.

[0034] A Chinese medicine hair-growth water containing hair follicle stem cell exosomes and having the effect of promoting hair growth, comprising a Chinese medicine water extract and a Chinese medicine alcohol extract; and further adding a surfactant, a solvent, a moisturizer and an antibacterial agent;

[0035] The Chinese medicine water extract is made from four samples of Eclipta prostrata, Angelica sinensis, Carthamus tinctorius, Astragalus membranaceus, Poria cocos, Dioscorea opposita, and Lycium barbarum.

[0036] The Chinese medicine alcohol extract is made of 6 kinds of Chinese medicine selected from the group consisting of Ligustrum lucidum, Rehmannia root, Radix Rehmanniae, Salvia miltiorrhiza, Rhizoma Chuanxiong, Paeonia lactiflora, Platycladus orientalis, and Polygonum multiflorum; the surfactant is selected from one or more of poloxamer, Tween 80, Span, and 1,3-butanediol;

[0037] The solvent is selected from pure water, glycerol, ethanol or a mixture of the two or more thereof;

[0038] The moisturizer is selected from panthenol, castor oil or a mixture of the two;

[0039] The antibacterial agent is selected from phenoxyethanol, propylene glycol, or a mixture of the two.

[0040] Example 1

[0041] (1) Add 10 times the amount of pure water to extract 10 g of each of the Chinese medicinal materials, including Eclipta prostrata, Angelica sinensis, Carthamus tinctorius, and Poria cocos, to obtain a Chinese medicinal water extract (20 mg / ml, calculated based on the amount of crude drug).

[0042] (2) Add 10 g each of Ligustrum lucidum fruit, Rehmannia root, Radix Rehmanniae glutinosae, Salvia miltiorrhiza, Rhizoma Chuanxiong and Platycladus orientalis leaves.

[0043] Add 10 times the amount of 95% ethanol for extraction to obtain a Chinese medicine alcohol extract (20 mg / ml, based on the amount of crude drug). (3) Mix the Chinese medicine water extract and the Chinese medicine alcohol extract in a volume ratio of 4:6 to obtain a Chinese medicine mixed extract. (4) Taking the total weight of the Chinese medicine hair growth liquid as 100%, take 30% of the Chinese medicine mixed extract, add a surfactant poloxamer (12%), stir and mix well to obtain phase A.

[0044] (5) Take pure water (51%), panthenol (1.5%), and propylene glycol (5%), mix them evenly, and mix them with the above phase A. Stir and mix them evenly at a certain stirring speed. Add propylene glycol (0.5%) during the mixing process and mix them thoroughly.

[0045] (6) The purified exosomes were taken and mixed with the prepared Chinese herbal hair growth water to make the exosome concentration reach 50 μg / ml.

[0046] Example 2

[0047] (1) Add 10 times the amount of pure water to extract 10 g of each of the Chinese medicinal materials Eclipta prostrata, Angelica sinensis, Carthamus tinctorius, and Astragalus membranaceus to obtain a Chinese medicinal water extract (20 mg / ml, calculated based on the amount of crude drug).

[0048] (2) Add 10 g each of Ligustrum lucidum fruit, Rehmannia root, Radix Rehmanniae glutinosae, Salvia miltiorrhiza, Rhizoma Chuanxiong and Polygonum multiflorum.

[0049] Add 10 times the amount of 95% ethanol for extraction to obtain a Chinese medicine alcohol extract (20 mg / ml, based on the amount of raw medicine). (3) Mix the Chinese medicine water extract and the Chinese medicine alcohol extract in a volume ratio of 4:6 to obtain a Chinese medicine mixed extract. (4) The preparation method of the Chinese medicine hair growth water in this example is the same as that in Example 1.

[0050] (5) The purified exosomes were taken and mixed with the prepared Chinese herbal hair growth water to make the exosome concentration reach 50 μg / ml.

[0051] Example 3

[0052] (1) Add 10 times the amount of pure water to extract 10 g of each of the Chinese medicinal materials, including Eclipta prostrata, Angelica sinensis, Poria cocos, and Dioscorea opposita, to obtain a Chinese medicinal water extract (20 mg / ml, calculated based on the amount of the raw drug).

[0053] (2) Add 10 g each of Ligustrum lucidum fruit, Rehmannia root, Radix Rehmanniae glutinosae, Salvia miltiorrhiza, Rhizoma Chuanxiong and Platycladus orientalis leaves.

[0054] Add 10 times the amount of 95% ethanol for extraction to obtain a Chinese medicine alcohol extract (20 mg / ml, based on the amount of raw medicine). (3) Mix the Chinese medicine water extract and the Chinese medicine alcohol extract in a volume ratio of 4:6 to obtain a Chinese medicine mixed extract. (4) The preparation method of the Chinese medicine hair growth water in this example is the same as that in Example 1.

[0055] (5) The purified exosomes were taken and mixed with the prepared Chinese herbal hair growth water to make the exosome concentration reach 50 μg / ml.

[0056] Example 4

[0057] (1) Add 10 times the amount of pure water to extract 10 g of each of the Chinese medicinal materials, including Eclipta prostrata, Carthamus tinctorius, Poria cocos, and Astragalus membranaceus, to obtain a Chinese medicinal water extract (20 mg / ml, calculated based on the amount of the raw drug).

[0058] (2) Add 10 g each of Ligustrum lucidum fruit, Rehmannia root, Radix Rehmanniae glutinosae, Salvia miltiorrhiza, Rhizoma Chuanxiong and Polygonum multiflorum.

[0059] Add 10 times the amount of 95% ethanol for extraction to obtain a Chinese medicine alcohol extract (20 mg / ml, based on the amount of crude drug). (3) Mix the Chinese medicine water extract and the Chinese medicine alcohol extract in a volume ratio of 4:6 to obtain a Chinese medicine mixed extract. (4) The preparation method of the Chinese medicine health-promoting hair water in this embodiment is the same as that in Example 1.

[0060] (5) The purified exosomes were taken and mixed with the prepared Chinese herbal hair growth water to make the exosome concentration reach 50 μg / ml.

[0061] Example 5

[0062] (1) Add 10 times the amount of pure water to extract 10 g of each of the Chinese medicinal materials, including angelica sinensis, safflower, poria cocos, and wolfberry, to obtain a Chinese medicinal water extract (20 mg / ml, calculated based on the amount of the raw drug).

[0063] (2) Add 10 times the amount of 95% ethanol to extract 10 g of each of Ligustrum lucidum fruit, Rehmannia root, Radix Rehmanniae, Rhizoma Chuanxiong, Platycladus orientalis, and Polygonum multiflorum to obtain a Chinese medicine alcohol extract (20 mg / ml, based on the amount of raw medicine).

[0064] (3) Mix the Chinese herbal medicine water extract and the Chinese herbal medicine alcohol extract in a volume ratio of 4:6 to obtain a Chinese herbal medicine mixed extract. (4) The preparation method of the Chinese herbal medicine hair growth water in this example is the same as that in Example 1.

[0065] (5) The purified exosomes were taken and mixed with the prepared Chinese herbal hair growth water to make the exosome concentration reach 50 μg / ml.

[0066] Example 6

[0067] (1) The preparation method of the mixed Chinese herbal extract of this embodiment is the same as that of Example 1.

[0068] (2) Taking the total weight of the Chinese herbal hair growth water as 100%, take the Chinese herbal mixed extract (30%), add the surfactant 1,3-butanediol (12%), stir and mix well to obtain phase A.

[0069] (3) Take pure water (51%), panthenol (1.5%), and glycerol (5%), mix them evenly, and mix them with the above phase A. Stir and mix them evenly at a certain stirring speed. During the mixing process, add phenoxyethanol (0.5%) and mix thoroughly.

[0070] (4) The purified exosomes were taken and mixed with the prepared Chinese herbal hair growth water to make the exosome concentration reach 50 μg / ml.

[0071] Example 7

[0072] (1) The preparation method of the mixed Chinese herbal extract of this embodiment is the same as that of Example 1.

[0073] (2) Taking the total weight of the Chinese herbal hair growth water as 100%, take the Chinese herbal mixed extract (30%), add the surfactant 1,3-butanediol (12%), stir and mix well to obtain phase A.

[0074] (3) Take pure water (51%), castor oil (1.5%), and propylene glycol (5%), mix them evenly, and mix them with the above-mentioned phase A. Stir and mix them evenly at a certain stirring speed. During the mixing process, add propylene glycol (0.5%) and mix them thoroughly.

[0075] (4) The purified exosomes were taken and mixed with the prepared Chinese herbal hair growth water to make the exosome concentration reach 50 μg / ml.

[0076] Effect experiment example

[0077] C57BL6 mouse experiments

[0078] The experimental conditions for the mouse experiments were as follows:

[0079] C57BL / 6 male mice were selected as animal models at 6 weeks old and adapted for 1 week. 1 cm2 of the back of the mice were shaved and depilatory cream was used to remove hair. Testosterone solution (0.2%, W / V) was prepared in 50% ethanol solution, and testosterone solution (0.1 ml / cm2) was applied topically to the depilatory area once a day for 14 consecutive days.

[0080] Drug administration began on the 5th day after modeling, and half an hour after testosterone administration. The model group was given 0.1 ml / cm2 of normal saline once a day; the positive control group was given 0.1 ml / cm2 of 5% minoxidil, and the drug administration group was given 0.1 ml / cm2 of traditional Chinese medicine hair growth water.

[0081] Photos of hair regeneration after hair loss were taken, as well as the coverage rate and density of regenerated hair. After 14 days, the mice were killed, and paraffin sections of the skin tissue from the hair loss area were taken to observe the hair follicles.

[0082] The hair growth of mice, the condition of hair follicles in the hair loss area and the health of regenerated hair are shown in the attached Figure 1 . From the comparison of experimental results, it can be seen that Chinese herbal hair-growing water can effectively promote the regeneration of hair in mice, increase the number of hair in hair follicle cells, and thicken the hair diameter, and it has a more significant effect on hair growth and fixation. The combination of Chinese herbal hair-growing water and exosomes has a better effect on promoting hair growth than Chinese herbal hair-growing water, and the health of the grown hair is better than that of the group given pure Chinese herbal hair-growing water.

[0083] In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front end", "rear end", "two ends", "one end", "the other end" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0084] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal connection of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0085] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A Chinese medicine hair-growth water containing hair follicle stem cell exosomes and having the effect of promoting hair growth, characterized in that: Including Chinese herbal medicine water extract and Chinese herbal medicine alcohol extract; in addition, surfactant, solvent, moisturizer and antibacterial agent are added; The Chinese medicine water extract is made from four samples of Eclipta prostrata, Angelica sinensis, Carthamus tinctorius, Astragalus membranaceus, Poria cocos, Dioscorea opposita, and Lycium barbarum. The Chinese medicine alcohol extract is made from six kinds of Chinese medicine selected from the group consisting of Ligustrum lucidum fruit, Rehmannia root, Radix Rehmanniae glutinosae, Salvia miltiorrhiza, Rhizoma Chuanxiong, Paeonia lactiflora, Platycladus orientalis leaves and Polygonum multiflorum.

2. The Chinese medicine hair-growth water containing hair follicle stem cell exosomes and having the effect of promoting hair growth according to claim 1, characterized in that: The surfactant is selected from one or more of poloxamer, Tween 80, Span, and 1,3-butanediol; The solvent is selected from pure water, glycerol, ethanol or a mixture of the two or more thereof; The moisturizer is selected from panthenol, castor oil or a mixture of the two; The antibacterial agent is selected from phenoxyethanol, propylene glycol, or a mixture of the two.

3. The Chinese medicine hair-growth water containing hair follicle stem cell exosomes and having the effect of promoting hair growth according to claim 2, characterized in that: The Chinese medicinal materials used in the aqueous extract of Chinese medicinal materials are Eclipta prostrata, Angelica sinensis, Carthamus tinctorius, and Poria cocos, with the mass of each medicinal material being 10 g; the Chinese medicinal materials used in the alcohol extract of Chinese medicinal materials are Fructus Ligustri Lucidi, Rehmannia glutinosa, Radix Rehmanniae glutinosae, Salvia miltiorrhiza, Rhizoma Chuanxiong, and Platycladus orientalis, with the mass of each medicinal material being 10 g; the surfactant accounts for 12%; the solvent, moisturizer, and antibacterial agent account for 56%, 1.5%, and 0.5%, respectively.

4. The Chinese medicine hair-growth water containing hair follicle stem cell exosomes and having the effect of promoting hair growth according to claim 2, characterized in that: The Chinese medicinal materials used in the aqueous extract of Chinese medicinal materials are Eclipta prostrata, Angelica sinensis, Carthamus tinctorius, and Astragalus membranaceus, with the mass of each medicinal material being 10 g; the Chinese medicinal materials used in the alcohol extract of Chinese medicinal materials are Fructus Ligustri Lucidi, Rehmannia glutinosa, Radix Rehmanniae glutinosae, Salvia miltiorrhiza, Rhizoma Chuanxiong, and Polygonum multiflorum, with the mass of each medicinal material being 10 g; the surfactant accounts for 12%; the solvent, moisturizer, and antibacterial agent account for 56%, 1.5%, and 0.5%, respectively.

5. The Chinese herbal hair-growth water containing hair follicle stem cell exosomes and having a hair-growth-promoting effect according to claim 2, characterized in that: The Chinese medicinal materials used in the aqueous extract of Chinese medicinal materials are Eclipta prostrata, Angelica sinensis, Poria cocos, and Chinese yam, with the mass of each medicinal material being 10 g; the Chinese medicinal materials used in the alcohol extract of Chinese medicinal materials are Ligustrum lucidum, Rehmannia root, Radix Rehmanniae, Salvia miltiorrhiza, Ligusticum chuanxiong, and Platycladus orientalis, with the mass of each medicinal material being 10 g; the surfactant accounts for 12%; the solvent, moisturizer, and antibacterial agent account for 56%, 1.5%, and 0.5%, respectively.

6. The Chinese medicine hair-growth water containing hair follicle stem cell exosomes and having the effect of promoting hair growth according to claim 2, characterized in that: The Chinese medicinal materials used in the aqueous extract of Chinese medicinal materials are Eclipta prostrata, Carthamus tinctorius, Poria cocos, and Astragalus membranaceus, with the mass of each medicinal material being 10 g; the Chinese medicinal materials used in the alcohol extract of Chinese medicinal materials are Fructus Ligustri Lucidi, Rehmannia glutinosa, Radix Rehmanniae glutinosae, Salvia miltiorrhiza, Rhizoma Chuanxiong, and Polygonum multiflorum, with the mass of each medicinal material being 10 g; the surfactant accounts for 12%; the solvent, moisturizer, and antibacterial agent account for 56%, 1.5%, and 0.5%, respectively.

7. The Chinese medicine hair-growth water containing hair follicle stem cell exosomes and having the effect of promoting hair growth according to claim 2, characterized in that: The Chinese medicinal materials used in the aqueous extract of Chinese medicinal materials are Angelica sinensis, safflower, Poria cocos, and Lycium barbarum, with the mass of each medicinal material being 10 g; the Chinese medicinal materials used in the alcohol extract of Chinese medicinal materials are Ligustrum lucidum, Rehmannia root, Rehmannia root, Ligusticum chuanxiong, Platycladus orientalis leaves, and Polygonum multiflorum, with the mass of each medicinal material being 10 g; the surfactant accounts for 12%; the solvent, moisturizer, and antibacterial agent account for 56%, 1.5%, and 0.5%, respectively.

8. The Chinese medicine hair-growth water containing hair follicle stem cell exosomes and having the effect of promoting hair growth according to claim 2, characterized in that: The Chinese medicinal materials used in the aqueous extract of Chinese medicinal materials are Angelica sinensis, safflower, Poria cocos, and Lycium barbarum, with the mass of each medicinal material being 10 g; the Chinese medicinal materials used in the alcohol extract of Chinese medicinal materials are Ligustrum lucidum, Rehmannia root, Rehmannia root, Ligusticum chuanxiong, Platycladus orientalis leaves, and Polygonum multiflorum, with the mass of each medicinal material being 10 g; the surfactant accounts for 12%; the solvent, moisturizer, and antibacterial agent account for 56%, 1.5%, and 0.5%, respectively.

9. A method for preparing a traditional Chinese medicine hair-growth water containing hair follicle stem cell exosomes and having a hair-growth-promoting effect according to any one of claims 1 to 8, characterized in that: ① Take 4 samples of Eclipta prostrata, Angelica sinensis, Carthamus tinctorius, Astragalus membranaceus, Poria cocos, Dioscorea opposita, and Lycium barbarum, each with a mass of 10 g, add 10 times the amount of pure water for extraction, and obtain a Chinese medicine water extract (extract 20 mg / ml, calculated based on the amount of crude drug); ② Take 6 samples of Ligustrum lucidum, Rehmannia root, Radix Rehmanniae, Salvia miltiorrhiza, Rhizoma Chuanxiong, Paeonia lactiflora, Platycladus orientalis, and Polygonum multiflorum, each with a weight of 10g, add 10 times the amount of 95% ethanol for extraction, and obtain the Chinese medicine alcohol extract (extract 20mg / ml, calculated based on the amount of crude drug); ③ Mix the Chinese herbal medicine water extract and the Chinese herbal medicine alcohol extract in a volume ratio of 4:6 to obtain a Chinese herbal medicine mixed extract; ④ Taking the total weight of the Chinese medicine hair growth liquid as 100%, take 30% of the Chinese medicine mixed extract, add 12% of the surfactant, stir and mix well to obtain phase A; ⑤ Take 56% of the solvent and 1.5% of the moisturizer and mix them at a temperature of 80°C-85°C, mix them with the above phase A, and stir and mix them at a speed of 200rpm / min-250rpm / min. During the mixing process, add 0.5% of the antibacterial agent and mix them thoroughly; ⑥Take the purified exosomes and mix them with the prepared Chinese medicine hair growth water to make the exosome concentration reach 50μg / ml.

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