Health food detection method

Abstract

The invention relates to the field of detection, in particular to a health-care food detection method, which comprises the following steps: pretreating a health-care food sample to obtain a determination solution, and detecting the determination solution through a liquid chromatography-tandem mass spectrometer to obtain the substance content in the health-care food, according to the ultrasonic cleaning device, the position of the volumetric flask in the ultrasonic cleaning machine can be adjusted according to the force required by particle decomposition at each position in the volumetric flask, so that the sound wave force at the position of each particle is changed, the force required by decomposition of the particles is achieved, the particles are fully decomposed, and the accuracy of subsequent liquid chromatography-tandem mass spectrometry detection can be ensured.

Description

Technical Field

[0001] The invention relates to the detection field, in particular to a health food detection method. Background Art

[0002] Health food is a special type of food that has the basic characteristics of food, can regulate human functions, and provide additional nutritional supplements to the human body. In order to ensure the safety of food, it is necessary to conduct corresponding testing of illegal additives for the claimed functions of health food; Currently, liquid chromatography-tandem mass spectrometry is mainly used to detect illegally added ingredients in health foods. Before the test, a test solution needs to be prepared. The prepared solution often contains solid sample particles, so it is necessary to use an ultrasonic cleaner for ultrasonic treatment to decompose the solid particles so that the material components therein can be fully dissolved. However, due to the different distribution of the acoustic field, the volumetric flask containing the sample solution is placed in a fixed position in the ultrasonic cleaner, and the acoustic wave force applied to different parts of the volumetric flask is different, so the force applied to the solid particles in the solution in the flask is also different. Currently, when pre-treating the sample before liquid quality testing, the operator often places the volumetric flask in a fixed position in the ultrasonic cleaner for ultrasonic treatment for a fixed time, so that the force applied to the solid particles in the volumetric flask is different, resulting in different decomposition degrees, making it difficult to fully decompose the solid particles, thereby affecting the accuracy of ingredient detection. Summary of the invention

[0003] Based on this, it is necessary to provide a health food detection method to address the above-mentioned problems.

[0004] The embodiment of the present invention is implemented by providing a health food detection method, the method comprising: S1: Take a solid sample into a volumetric flask and add solvent; S2: Determine the sonic force field of the ultrasonic cleaning machine when it is running based on historical data, control the robotic arm to grab the volumetric flask into the ultrasonic cleaning machine, and control the robotic arm to adjust the position of the volumetric flask in the sonic force field based on the force characteristics of the solid particles in the volumetric flask in the sonic force field, so that the solid particles are fully decomposed by the force; S3: adjust the volume of the volumetric flask to a fixed volume, transfer the solution in the volumetric flask to a centrifuge tube, and place the centrifuge tube in a centrifuge for centrifugation; S4: Take the supernatant from the centrifuge tube and filter it through a filter membrane to obtain a test solution; S5: adding the test solution to a liquid chromatography-tandem mass spectrometer to measure the concentration of each substance in the solution; S6: Calculate the content of each substance in the sample based on the measured concentration.

[0005] Preferably, the liquid chromatography-tandem mass spectrometer is connected to a computer device; the concentration of the substance measured by the liquid chromatography-tandem mass spectrometer is transmitted to the computer device; The computer equipment calculates the content of each substance using the following formula: Where X is the content of the substance, is the concentration of the substance in the test solution, m is the sampling mass, and V is the fixed volume of the test solution.

[0006] Preferably, determining the sonic force field of the ultrasonic cleaning machine when it is running based on historical data, controlling the mechanical arm to grab the volumetric flask into the ultrasonic cleaning machine, and controlling the mechanical arm to adjust the position of the volumetric flask in the sonic force field based on the force characteristics of the solid particles in the volumetric flask in the sonic force field includes: S21: Obtain a historical ultrasonic video, and determine the acoustic wave force field of the ultrasonic cleaning machine according to the vibration state of the solution in the volumetric flask in the historical ultrasonic video; S22: Control the robotic arm to clamp the volumetric flask, and then control the robotic arm to move the volumetric flask into the ultrasonic cleaning machine, start the ultrasonic cleaning machine, place the volumetric flask in the acoustic wave force field, and record the current ultrasonic video; S23: determining a critical force of particles of various particle sizes in the current ultrasound video, wherein particles of corresponding particle sizes can be further decomposed under the action of the critical force; S24: At set time intervals, the particle size of each particle in the volumetric flask and its position in the acoustic force field are identified, and the volumetric flask is moved according to the determined critical force so that the force applied to the most particles at their locations exceeds the corresponding critical force, so that each particle can be fully decomposed.

[0007] Preferably, the ultrasonic video is recorded by a camera; the same spatial coordinate system is set in each ultrasonic video; the vibration state includes vibration amplitude and vibration frequency; obtaining historical ultrasonic videos, and determining the acoustic wave force field of the ultrasonic cleaning machine according to the vibration state of the solution in the volumetric flask in the historical ultrasonic videos includes: S211: establishing a virtual space coordinate system corresponding to the space coordinate system in the ultrasonic video, and determining an ultrasonic region of the ultrasonic cleaning machine in the virtual space coordinate system, wherein the ultrasonic region is an action region of the ultrasonic cleaning machine, and the ultrasonic region is a space region; S212: Retrieving all historical ultrasound videos, and selecting a number of target ultrasound videos from the retrieved historical ultrasound videos, so that the merged area of ​​the inner space area of ​​the volumetric flask identified in each target ultrasound video can completely cover the ultrasound area; S213: for each target ultrasound video, identifying the vibration amplitude and vibration frequency of the solution at each position point in the bottle area in the target ultrasound video to determine the force score of the position point; S214: Mark each force score at a corresponding position point in the ultrasonic region to obtain an acoustic wave force field.

[0008] Preferably, each ultrasound video is marked with the recording start time of the ultrasound video; a plurality of target ultrasound videos are selected from the retrieved historical ultrasound videos so that the merged area of ​​the inner space area of ​​the volumetric flask identified in each target ultrasound video can completely cover the ultrasound area including: S2111: sorting the historical ultrasound videos according to the corresponding recording start time to obtain a first sequence, wherein the videos that are ranked higher in the first sequence have later corresponding recording start times; S2112: Selecting a first historical ultrasound video in the first sequence; S2113: identifying each first position point in the volumetric bottle in the selected historical ultrasound video, determining a second position point having the same coordinates as the first position point in the virtual space coordinate system, and rendering the second position point in a set color; S2114: determining the historical ultrasound video as the target ultrasound video, and determining whether the ultrasound region is completely rendered, and if so, stopping determining the target ultrasound video; S2115: If not, select the next historical ultrasound video in the first sequence, and execute steps S2113 to S2115 until the ultrasound area is completely rendered.

[0009] Preferably, the force score of the position point is determined by the following formula: in, is the intensity score, is the vibration amplitude, F is the vibration frequency, is the score adjustment factor.

[0010] Preferably, the critical force for determining particles of various particle sizes in the current ultrasound video includes: The particle size of the largest particle is identified as the maximum particle size; Determine a number of standard particle sizes from 0 to the maximum particle size, wherein the interval between every two adjacent standard particle sizes is a set interval; For each standard particle size, determine whether each particle with a particle size equivalent to the standard particle size is decomposed and the force score of the location point of the particle, and then determine the force score of the critical force of the particle of the standard particle size.

[0011] Preferably, determining whether each particle with a particle size equivalent to the standard particle size is decomposed and the force score of the location point of the particle, and then determining the force score of the critical force of the particle with the standard particle size includes: Arrange the force scores corresponding to all particles whose particle sizes are equivalent to the standard particle size in descending order to obtain a second sequence; For each intensity score in the second sequence, the first ratio corresponding to the intensity score is determined by the following formula: in, is the first ratio, is the number of intensity scores that come before this intensity score, The number of force scores further decomposed into the corresponding particles in the force scores sorted before the force score; The second ratio corresponding to the intensity score is determined by the following formula: in, For the second ratio, is the number of force scores that are sorted after this force score, is the number of unresolved force scores of the particles corresponding to the force scores sorted after this force score; determining all potential target scores in a second sequence, wherein a first ratio of the potential target scores is higher than a first preset ratio and a second ratio is higher than a second preset ratio; The ranking of each potential target score is identified, and the potential target score in the middle ranking is determined as the strength score of the critical force of the standard particle size.

[0012] Preferably, identifying the particle size of each particle in the volumetric flask and the position in the acoustic wave force field, and moving the volumetric flask according to the determined critical force so that the force applied to the most particles at their positions exceeds the corresponding critical force comprises: Identify the geometric center of the volumetric flask in the current ultrasound video; Generate a moving range of set size with the geometric center as the center; Taking each position point in the moving range as a candidate position point; For each candidate position point, a copy volumetric bottle is generated, and the copy volumetric bottle is moved to a position where the geometric center coincides with the candidate position point; Determine whether the force score of the position point of each particle in the replica volumetric flask exceeds the force score of the corresponding critical force. If so, determine the particle as the first particle and count the number of the first particles. Determine the candidate position point with the largest number of corresponding first particles as the target position point; Control the robotic arm to move the volumetric flask to the target position.

[0013] The invention provides a health food detection method, which comprises the following steps: taking a solid sample into a volumetric flask and adding a solvent; determining the sonic force field of an ultrasonic cleaning machine when the ultrasonic cleaning machine is in operation according to historical data, controlling a mechanical arm to grab the volumetric flask into the ultrasonic cleaning machine, and controlling the mechanical arm to adjust the position of the volumetric flask in the sonic force field according to the force characteristics of solid particles in the volumetric flask in the sonic force field, so that the solid particles are fully decomposed by force; fixing the volumetric flask, transferring the solution in the volumetric flask into a centrifuge tube, and placing the centrifuge tube on a centrifuge for centrifugation; taking the supernatant of the centrifuge tube, filtering through a filter membrane to obtain a determination solution; adding the determination solution into a liquid chromatography-tandem mass spectrometer to determine the concentration of each substance in the solution; and calculating the content of each substance in the sample according to the measured concentration; in the present application, in the process of ultrasonic treatment before detection, the position of the volumetric flask in the ultrasonic cleaning machine can be adjusted according to the force required for the decomposition of particles at each position in the volumetric flask, so that the sonic force at the position of each particle is changed, so as to achieve the force required for the decomposition thereof, so that the particles are fully decomposed, and thus the accuracy of the subsequent liquid chromatography-tandem mass spectrometry detection can be ensured. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 A flowchart of a health food detection method provided in one embodiment; Figure 2 A diagram of an application environment of a health food detection method provided in an embodiment; Figure 3 A schematic diagram of an ultrasonic region of a health food detection method provided in an embodiment; DETAILED DESCRIPTION In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0015] It is understood that the terms "first", "second", etc. used in the present invention may be used herein to describe various elements, but unless otherwise specified, these elements are not limited by these terms. These terms are only used to distinguish a first element from another element. For example, a first xx script may be referred to as a second xx script, and similarly, a second xx script may be referred to as a first xx script without departing from the scope of the present invention.

[0016] like Figure 1 As shown, in one embodiment, a health food detection method is proposed, the method comprising: S1: Take a solid sample into a volumetric flask and add solvent; S2: Determine the sonic force field of the ultrasonic cleaning machine when it is running based on historical data, control the robotic arm to grab the volumetric flask into the ultrasonic cleaning machine, and control the robotic arm to adjust the position of the volumetric flask in the sonic force field based on the force characteristics of the solid particles in the volumetric flask in the sonic force field, so that the solid particles are fully decomposed by the force; S3: adjust the volume of the volumetric flask to a fixed volume, transfer the solution in the volumetric flask to a centrifuge tube, and place the centrifuge tube in a centrifuge for centrifugation; S4: Take the supernatant from the centrifuge tube and filter it through a filter membrane to obtain a test solution; S5: adding the test solution to a liquid chromatography-tandem mass spectrometer to measure the concentration of each substance in the solution; S6: Calculate the content of each substance in the sample based on the measured concentration.

[0017] In this embodiment, steps S1, S3, S4 and other processes are manually performed by staff; step S2 is performed by computer equipment in collaboration with an ultrasonic cleaning machine and a robotic arm; step S5 is performed by staff operating a liquid chromatograph; step S6 is performed by computer equipment; the computer equipment may be an independent physical server or terminal, or a server cluster consisting of multiple physical servers, or a cloud server that provides basic cloud computing services such as cloud servers, cloud databases, cloud storage and CDN; Figure 2 As shown, the ultrasonic cleaning machine is connected to a computer device, and the computer device can control the start and shut down of the ultrasonic cleaning machine; a mechanical arm controlled by the computer device is arranged next to the ultrasonic cleaning machine, and the computer device can control the mechanical arm to grab a volumetric flask to adjust the position of the volumetric flask in the ultrasonic cleaning machine; at least one camera is also arranged next to the ultrasonic cleaning machine, and the camera is connected to the computer device, and the computer device can control the camera to collect ultrasonic video; further, the computer device is also connected to a liquid chromatography-tandem mass spectrometer, so that the detection results of the liquid chromatography-tandem mass spectrometer can be directly transmitted to the computer device, so that the computer device can calculate the content of the substance to be detected; In this embodiment, a solid sample is placed in a volumetric flask, and a solvent is added, i.e., a proper amount of a solid or semi-solid health food sample is mixed and ground; 1 g (accurate to 0.001 g) is accurately weighed and placed in a 50 mL volumetric flask, and a proper amount of methanol (i.e., solvent) is added; after ultrasonic extraction, the mixture is cooled to room temperature; the specific process of step S3-step S4 is to make up the volume with methanol, transfer the mixture to a 50 mL centrifuge tube, centrifuge at 4000 r / min for 5 min, filter the supernatant through a microporous filter membrane, and take the filtrate to obtain a test solution; In this embodiment, the acoustic force field represents the distribution of acoustic force generated by ultrasound. When acoustic forces at different positions act on a solution, the solution will vibrate with different frequencies and / or amplitudes, thereby causing the solution at the corresponding positions to produce different degrees of cavitation effects (bubbles are formed due to negative pressure generated by the vibration of the solution, and local impact forces are formed after the bubbles collapse, which can decompose particles), so that particles at the corresponding positions are subjected to decomposition forces. If the decomposition forces they are subjected to are greater than the critical force required for the decomposition of the particles, the particles can be decomposed. In the present application, during the ultrasonic treatment process before detection, the position of the volumetric flask in the ultrasonic cleaning machine can be adjusted according to the force required for decomposition of the particles at each position in the volumetric flask, so that the acoustic wave force at the position of each particle changes, so as to achieve the force required for its decomposition, so that it can be fully decomposed, thereby ensuring the accuracy of the subsequent liquid chromatography-tandem mass spectrometry detection.

[0018] As a preferred embodiment, the liquid chromatography-tandem mass spectrometer is connected to a computer device; the concentration of the substance measured by the liquid chromatography-tandem mass spectrometer is transmitted to the computer device; The computer equipment calculates the content of each substance using the following formula: Where X is the content of the substance, is the concentration of the substance in the test solution, m is the sampling mass, and V is the fixed volume of the test solution.

[0019] In this embodiment, the unit of C is g / mL, the unit of V is mL, and the unit of m is g; this method can be used to detect 98 illegally added substances such as sildenafil, vardenafil, and lodenafil in health foods; standard solutions need to be prepared before determination, and the specific process is to accurately weigh 10 mg (accurate to 0.01 mg) of lodenafil carbonate, vardenafil dimer, and sildenafil dimer standard products, dissolve and dilute to 100 mL with hydrochloric acid methanol solution (5.2.2), and shake well; weigh 10 mg (accurate to 0.01 mg) of the remaining 95 standards, dissolve and dilute to 100 mL with methanol, and shake well. Prepare a standard stock solution with a concentration of 100 μg / mL and store it at -18°C away from light; accurately aspirate 0.1 mL of each standard stock solution (100 μg / mL), dilute and dilute to 10 mL with methanol, and shake well to prepare a mixed standard intermediate working solution with a concentration of 1 μg / mL. Accurately pipette 0.02mL, 0.05mL, 0.1mL, 0.2mL, and 0.5mL of the mixed standard intermediate working solution (1μg / mL), dilute it with methanol and make up to 10mL to prepare a series of mixed standard working solutions with concentrations of 2ng / mL, 5ng / mL, 10ng / mL, 20ng / mL, and 50ng / mL.

[0020] The mixed standard series working solutions are measured in order from small to large concentrations. During the measurement process, for each substance, the instrument draws a standard curve with the substance concentration as the horizontal coordinate and the peak area corresponding to the substance as the vertical coordinate; then the measurement solution is measured to obtain the chromatographic curve of the test solution, identify the peak area of ​​the substance in the chromatographic curve, and compare the area with the standard curve to obtain the concentration of the substance in the measurement solution.

[0021] The chromatographic conditions tested included: Chromatographic column: C18 chromatographic column (particle size 1.8μm, 3.0mm×150mm), or equivalent; Temperature: 35℃; Injection volume: 2 μL; Mobile phase A: 0.1% formic acid in water; Mobile phase B: acetonitrile; Flow rate: 0.3 mL / min; Mass spectrometry conditions included: Ion source: electrospray ion source, temperature 150°C; Scanning mode: positive ion mode and negative ion mode; Collision gas: argon; Desolventization temperature: 500℃; Desolventizing gas flow rate: 1000L / h; Capillary voltage: 1.0 kV.

[0022] As a preferred embodiment, determining the acoustic wave force field of the ultrasonic cleaning machine when it is running based on historical data, controlling the mechanical arm to grab the volumetric flask into the ultrasonic cleaning machine, and controlling the mechanical arm to adjust the position of the volumetric flask in the acoustic wave force field based on the force characteristics of the solid particles in the volumetric flask in the acoustic wave force field includes: S21: Obtain a historical ultrasonic video, and determine the acoustic wave force field of the ultrasonic cleaning machine according to the vibration state of the solution in the volumetric flask in the historical ultrasonic video; S22: Control the robotic arm to clamp the volumetric flask, and then control the robotic arm to move the volumetric flask into the ultrasonic cleaning machine, start the ultrasonic cleaning machine, place the volumetric flask in the acoustic wave force field, and record the current ultrasonic video; S23: determining a critical force of particles of various particle sizes in the current ultrasound video, wherein particles of corresponding particle sizes can be further decomposed under the action of the critical force; S24: At set time intervals, the particle size of each particle in the volumetric flask and its position in the acoustic force field are identified, and the volumetric flask is moved according to the determined critical force so that the force applied to the most particles at their locations exceeds the corresponding critical force, so that each particle can be fully decomposed.

[0023] In this embodiment, the set duration can be 3 seconds, 5 seconds or other durations; the historical data includes historical ultrasonic videos, which are stored in the computer device; since the operating mode of the ultrasonic cleaning machine is fixed, the acoustic wave force field it generates is also the same, so the acoustic wave force field generated by the ultrasonic cleaning machine during operation can be inferred through the action characteristics in the historical ultrasonic video; furthermore, in the current ultrasonic video, some of the particles in the volumetric flask are decomposed and some are not decomposed, so the force applied to the decomposed particles can be inferred through the position of the acoustic wave force field where the decomposed particles are located, and then the critical force of decomposition can be obtained, and then the position of the volumetric flask is adjusted according to the position of the undecomposed particles, so that the force applied to the undecomposed particles can also reach its critical force, thereby achieving full decomposition of all particles.

[0024] As a preferred embodiment, the ultrasonic video is recorded by a camera; the same spatial coordinate system is set in each ultrasonic video; the vibration state includes vibration amplitude and vibration frequency; the historical ultrasonic video is obtained, and the acoustic wave force field of the ultrasonic cleaning machine is determined according to the vibration state of the solution in the volumetric flask in the historical ultrasonic video, including: S211: establishing a virtual space coordinate system corresponding to the space coordinate system in the ultrasonic video, and determining an ultrasonic region of the ultrasonic cleaning machine in the virtual space coordinate system, wherein the ultrasonic region is an action region of the ultrasonic cleaning machine, and the ultrasonic region is a space region; S212: Retrieving all historical ultrasound videos, and selecting a number of target ultrasound videos from the retrieved historical ultrasound videos, so that the merged area of ​​the inner space area of ​​the volumetric flask identified in each target ultrasound video can completely cover the ultrasound area; S213: for each target ultrasound video, identifying the vibration amplitude and vibration frequency of the solution at each position point in the bottle area in the target ultrasound video to determine the force score of the position point; S214: Mark each force score at a corresponding position point in the ultrasonic region to obtain an acoustic wave force field.

[0025] Each ultrasound video is marked with the recording start time of the ultrasound video; a number of target ultrasound videos are selected from the retrieved historical ultrasound videos so that the merged area of ​​the inner space area of ​​the volumetric flask identified in each target ultrasound video can completely cover the ultrasound area including: S2111: sorting the historical ultrasound videos according to the corresponding recording start time to obtain a first sequence, wherein the videos that are ranked higher in the first sequence have later corresponding recording start times; S2112: Selecting a first historical ultrasound video in the first sequence; S2113: identifying each first position point in the volumetric bottle in the selected historical ultrasound video, determining a second position point having the same coordinates as the first position point in the virtual space coordinate system, and rendering the second position point in a set color; S2114: determining the historical ultrasound video as the target ultrasound video, and determining whether the ultrasound region is completely rendered, and if so, stopping determining the target ultrasound video; S2115: If not, select the next historical ultrasound video in the first sequence, and execute steps S2113 to S2115 until the ultrasound area is completely rendered.

[0026] The force score of a location point is determined by the following formula: in, is the intensity score, is the vibration amplitude, F is the vibration frequency, is the score adjustment factor.

[0027] In this embodiment, at least one camera is arranged beside the ultrasonic cleaning machine for recording ultrasonic videos (i.e., videos recorded each time ultrasonic treatment is performed); the origin of the spatial coordinate system in each ultrasonic video can be set at the same position of the ultrasonic cleaning machine, such as a corner point of the ultrasonic cleaning machine; the same spatial coordinate system is arranged in each ultrasonic video, so that the spatial coordinates of the same position in different ultrasonic videos are consistent; In this embodiment, if Figure 3 As shown, a coordinate range of the ultrasonic region is preset, such as (0~20, 0~20, 0~10), and the region consisting of all coordinate points covered by the range is determined in the spatial coordinate system, namely, the ultrasonic region; due to the uneven distribution of the color of the solution and the particles in the solution, the space region inside the volumetric flask in the ultrasonic video cannot be fully recognized by the camera. In this embodiment, for each ultrasonic video, only the space region inside the bottle that can be recognized is recognized (that is, the camera can monitor the region inside the bottle where the particles vibrate in the solution as the solution vibrates); In this embodiment, when rendering the second position point, if the second position point has been rendered, the position point will not be rendered repeatedly; the first position point and the second position point are three-dimensional units occupying a certain space size (such as a cube with a side length of 0.1 cm), and the corresponding force score can be determined by monitoring the movement of the solution and particles in the three-dimensional unit, and the coordinates of the first position point and the second position point are the coordinates corresponding to the center of the three-dimensional unit, that is, the coordinates of the position point; in this embodiment, the vibration characteristics of the particles can characterize the vibration characteristics of the solution, and the vibration amplitude can be determined based on the average of the back-and-forth movement distances of each particle in the three-dimensional unit, and the vibration frequency can be determined by the average of the number of times each particle moves back and forth in a unit time; In this embodiment, the set color can be red, and after the ultrasonic area is completely rendered, the corresponding color depth can be adjusted according to the force score corresponding to each second position point (different color depths correspond to different force scores), so that the sound wave force field can be presented intuitively; in this embodiment, after the sound wave force field is determined, for each position point in the current ultrasonic video, the second position point of the corresponding coordinates can be found in the virtual space coordinate system, so as to determine the force score of the position point; the determined sound wave force field can also be directly moved to the corresponding position in the current ultrasonic video, and then the force scores corresponding to each position point in the video can be directly determined.

[0028] As a preferred embodiment, the critical force for determining particles of various particle sizes in the current ultrasound video includes: The particle size of the largest particle is identified as the maximum particle size; Determine a number of standard particle sizes from 0 to the maximum particle size, wherein the interval between every two adjacent standard particle sizes is a set interval; For each standard particle size, determine whether each particle with a particle size equivalent to the standard particle size is decomposed and the force score of the location point of the particle, and then determine the force score of the critical force of the particle of the standard particle size.

[0029] Determining whether each particle with a particle size equivalent to the standard particle size is decomposed and the force score of the location point of the particle, and then determining the force score of the critical force of the particle of the standard particle size includes: Arrange the force scores corresponding to all particles whose particle sizes are equivalent to the standard particle size in descending order to obtain a second sequence; For each intensity score in the second sequence, the first ratio corresponding to the intensity score is determined by the following formula: in, is the first ratio, is the number of intensity scores that come before this intensity score, The number of force scores further decomposed into the corresponding particles in the force scores sorted before the force score; The second ratio corresponding to the intensity score is determined by the following formula: in, For the second ratio, is the number of force scores that are sorted after this force score, is the number of unresolved force scores of the particles corresponding to the force scores sorted after this force score; determining all potential target scores in a second sequence, wherein a first ratio of the potential target scores is higher than a first preset ratio and a second ratio is higher than a second preset ratio; The ranking of each potential target score is identified, and the potential target score in the middle ranking is determined as the strength score of the critical force of the standard particle size.

[0030] In this embodiment, for example, the interval can be set to 0.5mm. If the maximum particle size is 2mm, five standard particle sizes of 0, 0.5mm, 1mm, 1.5mm and 2mm can be determined; if the particle size of a particle is between two standard particle sizes (for example, 0.8mm is between 0.5mm and 1mm), the particle size of the particle is equivalent to the larger standard particle size (i.e. 1mm); each standard particle size corresponds to multiple particles, and the positions of the particles are different, and the force scores corresponding to the forces they receive are also different, so some particles will decompose, while others will not; theoretically, in the second sequence, the particles corresponding to the force scores before the force score corresponding to the critical force can all be decomposed, while the particles corresponding to the force scores after that will not be decomposed; considering the collection In order to prevent data errors (i.e., some particles that should have been decomposed are not actually decomposed, while particles that should not have been decomposed are actually decomposed), a first preset ratio (e.g., 95%) and a second preset ratio (e.g., 95%) are preset. When the first ratio corresponding to the strength score reaches the first preset ratio and the second ratio reaches the second preset ratio, the strength score is very close to the strength score of the critical force and can be regarded as the strength score of the critical force. If there are multiple strength scores that meet this condition, the middle ranking (i.e., the middle ranking in the ranking of strength scores that meet the conditions, for example, if 5 strength scores meet the standard, the third strength score that meets the standard is taken, and if 6 strength scores meet the standard, the third or third strength score is taken) strength score is taken as the strength score of the critical force, which has higher accuracy.

[0031] As a preferred embodiment, identifying the particle size of each particle in the volumetric flask and the position in the acoustic wave force field, and moving the volumetric flask according to the determined critical force so that the force applied to the most particles at their positions exceeds the corresponding critical force includes: Identify the geometric center of the volumetric flask in the current ultrasound video; Generate a moving range of set size with the geometric center as the center; Taking each position point in the moving range as a candidate position point; For each candidate position point, a copy volumetric bottle is generated, and the copy volumetric bottle is moved to a position where the geometric center coincides with the candidate position point; Determine whether the force score of the position point of each particle in the replica volumetric flask exceeds the force score of the corresponding critical force. If so, determine the particle as the first particle and count the number of the first particles. Determine the candidate position point with the largest number of corresponding first particles as the target position point; Control the robotic arm to move the volumetric flask to the target position.

[0032] In this embodiment, the moving range of the set size can be a cube range of the set side length; the position point in the set range is the above-mentioned cubic unit; the copy volumetric flask is a volumetric flask of the same size as the volumetric flask in the video, and the particles therein also correspond to the original volumetric flask; the copy volumetric flask is moved to a position where the geometric center coincides with the alternative position point, that is, the geometric center of the copy volumetric flask coincides with the center of the position point; in this embodiment, by setting the set range, the excessive movement of the volumetric flask can be reduced, thereby changing the position of the particles in the volumetric flask to improve the accuracy of decomposition; this embodiment can determine the position of the volumetric flask that can decompose the most particles at the same time by simulating movement, thereby greatly improving the decomposition efficiency of the particles.

[0033] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0034] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A method for detecting health food, characterized in that: The method comprises: S1: Take a solid sample into a volumetric flask and add solvent; S2: Determine the sonic force field of the ultrasonic cleaning machine when it is running based on historical data, control the robotic arm to grab the volumetric flask into the ultrasonic cleaning machine, and control the robotic arm to adjust the position of the volumetric flask in the sonic force field based on the force characteristics of the solid particles in the volumetric flask in the sonic force field, so that the solid particles are fully decomposed by the force; S3: adjust the volume of the volumetric flask to a fixed volume, transfer the solution in the volumetric flask to a centrifuge tube, and place the centrifuge tube in a centrifuge for centrifugation; S4: Take the supernatant from the centrifuge tube and filter it through a filter membrane to obtain a test solution; S5: adding the test solution to a liquid chromatography-tandem mass spectrometer to measure the concentration of each substance in the solution; S6: Calculate the content of each substance in the sample based on the measured concentration.

2. The method according to claim 1, characterized in that The liquid chromatography-tandem mass spectrometer is connected to a computer device; the concentration of the substance measured by the liquid chromatography-tandem mass spectrometer is transmitted to the computer device; The computer equipment calculates the content of each substance using the following formula: Where X is the content of the substance, is the concentration of the substance in the test solution, m is the sampling mass, and V is the fixed volume of the test solution.

3. The method according to claim 1, characterized in that Determine the sonic force field of the ultrasonic cleaning machine when it is running based on historical data, control the robotic arm to grab the volumetric flask into the ultrasonic cleaning machine, and control the robotic arm to adjust the position of the volumetric flask in the sonic force field based on the force characteristics of the solid particles in the volumetric flask in the sonic force field, including: S21: Obtain a historical ultrasonic video, and determine the acoustic wave force field of the ultrasonic cleaning machine according to the vibration state of the solution in the volumetric flask in the historical ultrasonic video; S22: Control the robotic arm to clamp the volumetric flask, and then control the robotic arm to move the volumetric flask into the ultrasonic cleaning machine, start the ultrasonic cleaning machine, place the volumetric flask in the acoustic wave force field, and record the current ultrasonic video; S23: determining a critical force of particles of various particle sizes in the current ultrasound video, wherein particles of corresponding particle sizes can be further decomposed under the action of the critical force; S24: At set time intervals, the particle size of each particle in the volumetric flask and its position in the acoustic force field are identified, and the volumetric flask is moved according to the determined critical force so that the force applied to the most particles at their locations exceeds the corresponding critical force, so that each particle can be fully decomposed.

4. The method according to claim 3, characterized in that: The ultrasonic video is recorded by a camera; the same spatial coordinate system is set in each ultrasonic video; the vibration state includes the vibration amplitude and the vibration frequency; the historical ultrasonic video is obtained, and the acoustic wave force field of the ultrasonic cleaning machine is determined according to the vibration state of the solution in the volumetric flask in the historical ultrasonic video, including: S211: establishing a virtual space coordinate system corresponding to the space coordinate system in the ultrasonic video, and determining an ultrasonic region of the ultrasonic cleaning machine in the virtual space coordinate system, wherein the ultrasonic region is an action region of the ultrasonic cleaning machine, and the ultrasonic region is a space region; S212: Retrieving all historical ultrasound videos, and selecting a number of target ultrasound videos from the retrieved historical ultrasound videos, so that the merged area of ​​the inner space area of ​​the volumetric flask identified in each target ultrasound video can completely cover the ultrasound area; S213: for each target ultrasound video, identifying the vibration amplitude and vibration frequency of the solution at each position point in the bottle area in the target ultrasound video to determine the force score of the position point; S214: Mark each force score at a corresponding position point in the ultrasonic region to obtain an acoustic wave force field.

5. The method according to claim 4, characterized in that Each ultrasound video is marked with the recording start time of the ultrasound video; a number of target ultrasound videos are selected from the retrieved historical ultrasound videos so that the merged area of ​​the inner space area of ​​the volumetric flask identified in each target ultrasound video can completely cover the ultrasound area including: S2111: sorting the historical ultrasound videos according to the corresponding recording start time to obtain a first sequence, wherein the videos that are ranked higher in the first sequence have later corresponding recording start times; S2112: Selecting a first historical ultrasound video in the first sequence; S2113: identifying each first position point in the volumetric bottle in the selected historical ultrasound video, determining a second position point having the same coordinates as the first position point in the virtual space coordinate system, and rendering the second position point in a set color; S2114: determining the historical ultrasound video as the target ultrasound video, and determining whether the ultrasound region is completely rendered, and if so, stopping determining the target ultrasound video; S2115: If not, select the next historical ultrasound video in the first sequence, and execute steps S2113 to S2115 until the ultrasound area is completely rendered.

6. The method according to claim 4, characterized in that The force score of a location point is determined by the following formula: in, is the intensity score, is the vibration amplitude, F is the vibration frequency, is the score adjustment factor.

7. The method according to claim 5, characterized in that The critical forces for determining particles of various sizes in current ultrasound videos include: The particle size of the largest particle is identified as the maximum particle size; Determine a number of standard particle sizes from 0 to the maximum particle size, wherein the interval between every two adjacent standard particle sizes is a set interval; For each standard particle size, determine whether each particle with a particle size equivalent to the standard particle size is decomposed and the force score of the location point of the particle, and then determine the force score of the critical force of the particle of the standard particle size.

8. The method according to claim 7, characterized in that Determining whether each particle with a particle size equivalent to the standard particle size is decomposed and the force score of the location point of the particle, and then determining the force score of the critical force of the particle of the standard particle size includes: Arrange the force scores corresponding to all particles whose particle sizes are equivalent to the standard particle size in descending order to obtain a second sequence; For each intensity score in the second sequence, the first ratio corresponding to the intensity score is determined by the following formula: in, is the first ratio, is the number of intensity scores that come before this intensity score, The number of force scores further decomposed into the corresponding particles in the force scores sorted before the force score; The second ratio corresponding to the intensity score is determined by the following formula: in, For the second ratio, is the number of force scores that are sorted after this force score, is the number of unresolved force scores of the particles corresponding to the force scores sorted after this force score; determining all potential target scores in a second sequence, wherein a first ratio of the potential target scores is higher than a first preset ratio and a second ratio is higher than a second preset ratio; The ranking of each potential target score is identified, and the potential target score in the middle ranking is determined as the strength score of the critical force of the standard particle size.

9. The method according to claim 8, characterized in that Identify the particle size of each particle in the volumetric flask and its position in the acoustic force field, and move the volumetric flask according to the determined critical force so that the force on the most particles at their locations exceeds the corresponding critical force, including: Identify the geometric center of the volumetric flask in the current ultrasound video; Generate a moving range of set size with the geometric center as the center; Taking each position point in the moving range as a candidate position point; For each candidate position point, a copy volumetric bottle is generated, and the copy volumetric bottle is moved to a position where the geometric center coincides with the candidate position point; Determine whether the force score of the position point of each particle in the replica volumetric flask exceeds the force score of the corresponding critical force. If so, determine the particle as the first particle and count the number of the first particles. Determine the candidate position point with the largest number of corresponding first particles as the target position point; Control the robotic arm to move the volumetric flask to the target position.

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