Cryopreservation container capable of adjusting internal air pressure

Abstract

The invention discloses a cryopreservation container capable of adjusting internal air pressure, and mainly relates to the technical field of cryopreservation. Comprising a partition plate and a cover plate which are arranged on the refrigerator, a plurality of notches and through holes communicated with the notches and the outside are formed in the partition plate, protruding plates making contact with the notches are arranged at the ends of a pipe body, a pipe cover makes contact with the cover plate, the protruding plates partition circulation of a freezing cavity and the through holes, and a storage cavity communicated with the through holes is formed in the pipe body. A piston is slidably connected into the storage cavity, a push rod is arranged on one side of the piston, the push rod extends to the outer side of the pipe body and is provided with a push plate, a push block is slidably connected to the refrigerator, grooves are formed in the push block, the upper side and the lower side of the push plate make contact with the grooves respectively, and a cam driving the push block to move in the transverse direction is rotatably connected to the refrigerator. The cryopreservation tube has the beneficial effects that the problem that the air pressure in the cryopreservation tube is changed due to external temperature change is solved, the sublimation phenomenon in the cryopreservation process is reduced, and the sample cryopreservation effect is improved.

Description

Technical Field

[0001] The invention relates to the technical field of cryopreservation, in particular to a cryopreservation container with adjustable internal air pressure. Background Art

[0002] Cryotubes, also called freezing tubes, are mainly used for low-temperature transportation and storage of biological materials. Cryotubes are generally used for low-temperature preservation of laboratory cells. They are often used in biological and medical experiments, and are also used in experiments in other industries such as food. Existing cryotubes include a tube body and a tube cover. The tube cover is installed on the tube body. When samples need to be refrigerated at ultra-low temperatures for a long time, the samples will be transferred from a room at about 4°C or room temperature to a refrigerator at -80°C. In this process, the air shrinks under the action of low temperature. During the shrinkage of the air, the pressure of the cryotube will decrease. The decrease in pressure will generate negative pressure, which will increase the sublimation of the sample, affecting the freezing effect of the sample to a certain extent. Summary of the invention

[0003] The purpose of the present invention is to provide a cryopreservation container with adjustable internal air pressure, so as to solve the problem that the air pressure inside the cryopreservation tube changes due to changes in external temperature, reduce the occurrence of sublimation during the cryopreservation process, and improve the effect of freezing samples.

[0004] In order to achieve the above-mentioned purpose, the invention is implemented through the following technical solutions:

[0005] A freezing container with adjustable internal air pressure, comprising a refrigerator and a plurality of freezing tubes, wherein the refrigerator is provided with a partition and a cover plate, wherein the partition and the cover plate form a plurality of freezing chambers with the refrigerator, wherein the plurality of freezing tubes are arranged in parallel in the freezing chamber, wherein the freezing tubes comprise a tube body and a tube cover, wherein the partition is provided with a plurality of notches and through holes connecting the notches and the outside, wherein the end of the tube body is provided with a convex plate in contact with the notches, wherein the tube cover is in contact with the cover plate, wherein the convex plate blocks the flow between the freezing chamber and the through holes, wherein the tube body is provided with a storage chamber in communication with the through holes, wherein a piston is slidably connected in the storage chamber, wherein a push rod is provided on one side of the piston, wherein the push rod extends to the outside of the tube body and is provided with a push plate;

[0006] A push block is symmetrically connected to the refrigerator in a vertical sliding manner. The push block is provided with a groove. The upper and lower sides of the push plate are respectively in contact with the groove. A cam is rotatably connected to the refrigerator to drive the push block to move horizontally.

[0007] Furthermore, a slide groove is provided on the refrigerator, and a slider that is in sliding contact with the slide groove is provided on one side of the push block, first planes are provided on the left and right sides of the cam, and second planes are provided on the front and rear sides of the cam, respectively, the spacing between the two first planes is smaller than the spacing between the two second planes, and an arc surface is provided between the first plane and the second plane, the first plane, the arc surface and the second plane are in contact with one side of the slider in turn, and drive the slider to slide horizontally in the slide groove, and a reset block that is in contact with the other side of the slider is slidably connected in the slide groove, and a first spring is provided between the reset block and the refrigerator.

[0008] Furthermore, the slider is provided with a plurality of limit grooves, the upper and lower sides of the cam are respectively in contact with the limit grooves, and limit the slider from sliding vertically in the grooves, and the upper and lower sides of the arc surface are respectively provided with inclined surfaces in contact with the limit grooves, and drive the slider to slide horizontally in the grooves.

[0009] Furthermore, a plurality of first guide pillars are provided on one side of the cam, which is rotatably connected to a drive shaft on the refrigerator, a plurality of spiral grooves are spirally provided on the drive shaft, a flat groove is provided between two adjacent spiral grooves, and the plurality of spiral grooves are in contact with the first guide pillars in sequence and drive the cam to rotate on the refrigerator, and the flat grooves are in contact with the first guide pillars and limit the cam from rotating on the refrigerator.

[0010] Furthermore, the refrigerator is provided with a first motor, a driving gear is provided at a movable end of the first motor, and a driven gear meshing with the driving gear is provided at the end of the driving shaft.

[0011] Furthermore, a plurality of second guide pillars are provided on one side of the slider, and a turntable is rotatably connected to the refrigerator. An arc block and a vertical pillar are provided on the turntable. The arc block contacts with the plurality of second guide pillars at the same time and limits the slider from sliding vertically in the slide groove. The vertical pillar contacts with the plurality of second guide pillars in turn and drives the slider to slide vertically in the slide groove.

[0012] Furthermore, the refrigerator is provided with a second motor, and a movable end of the second motor is connected to the turntable.

[0013] Furthermore, a temperature sensor is provided in the refrigerator.

[0014] Furthermore, a sealing strip is provided between the push plate and the groove, a pressure plate in contact with the tube cover is slidably connected to the cover plate, a plurality of guide columns are provided on the pressure plate, a plurality of L-shaped blocks in sliding connection with the guide columns are provided on the cover plate, a protrusion in contact with the L-shaped block is provided at the end of the guide column, and a second spring is provided between the guide column and the cover plate.

[0015] Compared with the prior art, the beneficial effects of the present invention are:

[0016] 1. Move the piston of the cryotube to the bottom of the tube body in advance, open the tube cover and put the sample into the cryotube, then cover the tube cover and put the cryotube into the refrigerator, let the push rod and the push plate pass through the through holes provided on the partition respectively, so that the convex plate contacts the notch, repeat the above actions in sequence to put all the cryotubes into the refrigerator, and then cover the cover plate on the top of the refrigerator to form a sealed chamber in the freezing chamber to prevent subsequent cold air leakage and affect the effect of freezing samples. At the same time, the cover plate contacts the tube cover to limit the movement of the cryotube in the refrigerator, so that the position of the piston can be better adjusted later, ensuring that the internal air pressure of the cryotube is always maintained in a stable range, thereby reducing the occurrence of sample sublimation during the freezing process and improving the effect of freezing samples;

[0017] 2. The two push blocks are driven by the cam to move horizontally. Since the push plates on each cryotube are located on the same horizontal line at this time, several push plates enter the grooves provided on the push blocks at the same time, and then gradually input cold air into the freezing chamber. At the same time, according to the temperature change in the freezing chamber, the push blocks are gradually moved vertically. Since the upper and lower sides of several push plates are in contact with the grooves provided on the push blocks at the same time, power is transmitted through the corresponding push rods, driving the corresponding pistons to slide upward in the tube sleeves, thereby changing the volume of the storage chamber, adjusting the air pressure in the storage chamber, compensating for the influence of the external temperature change of the cryotube on the internal air pressure of the storage chamber, so that the internal air pressure of the cryotube is always maintained in a stable range, thereby reducing the occurrence of sample sublimation during the freezing process and improving the effect of freezing samples;

[0018] In addition, the push block can drive several push plates to move at the same time, without moving the push plates one by one, thereby simplifying the operation steps of moving multiple pistons and improving the efficiency of freezing samples; in addition, since different samples have different stabilities under the same air pressure, the corresponding push plates can be driven to move by several push blocks to change the air pressure range of the cryopreservation tubes storing different samples, thereby meeting the storage requirements of different samples and further improving the effect of freezing samples;

[0019] 3. Before placing the cryopreservation tube, the second plane of the cam contacts the slider, and the two push blocks are located at the outermost side. After all the cryopreservation tubes containing samples are placed in the refrigerator, the driving shaft is driven to rotate on the refrigerator through the cooperation between the first motor, the driving gear, and the driven gear. The driving cam is driven to rotate on the refrigerator through the cooperation between the spiral groove and the first guide column, so that the contact surface between the cam and the slider gradually switches from the second plane to the arc surface. At the same time, under the action of the first spring rebound force, the reset block is driven to slide in the slide groove, and the two sliders are driven to gradually move inward until the push plates provided on the cryopreservation tubes enter the grooves provided on the corresponding push blocks;

[0020] Then, through the cooperation between the second motor, the turntable, the arc block, and the second guide column, the restriction of the slider sliding vertically in the slide groove is released, and then through the cooperation between the column and the second guide column, the slider is driven to slide vertically in the slide groove, and at the same time, through the cooperation between the cam, the arc surface, the slider, the inclined surface, the limit groove, and the first spring, the slider can move relative to the cam, and then the thrust is transmitted through the push block, the push plate, and the push rod, and at the same time, a number of corresponding pistons are driven to slide on the tube body, changing the air pressure range of the cryopreservation tubes storing different samples, thereby meeting the storage requirements of different samples and further improving the effect of freezing samples; then, the temperature in the freezing chamber is gradually reduced, and according to the temperature change in the freezing chamber, the corresponding push block is driven to move vertically by the second motor, thereby changing the volume of the corresponding cryopreservation tube storage chamber, adjusting the air pressure in the storage chamber, compensating for the influence of the external temperature change of the cryopreservation tube on the internal air pressure of the storage chamber, so that the internal air pressure of the cryopreservation tube is always maintained in a stable range, thereby reducing the occurrence of sample sublimation during the freezing process and improving the effect of freezing samples;

[0021] 4. After the temperature of the freezing chamber is constant, the cam is further driven to rotate by the first motor until the first plane contacts the slider. At this time, when the external force drives the slider to move vertically up and down, since the contact surfaces of the upper and lower sides of the cam and the limit groove are parallel to each other, no component force can be generated to drive the slider to move toward both sides. The generated resistance will limit the movement of the slider relative to the cam, thereby preventing the external force from accidentally driving the slider up and down, causing the air pressure of the storage chamber inside the cryopreservation tube to change, thereby ensuring that the air pressure inside the cryopreservation tube is always maintained in a stable range during the freezing process, reducing the occurrence of sample sublimation during the freezing process, and improving the effect of freezing samples;

[0022] During the freezing process, the arc block will contact with several second guide pillars again, and the resistance generated by the contact between the two will further limit the vertical sliding of the slider in the slide groove, thereby improving the effect of freezing the sample; at the same time, the first column will also move to the flat groove provided between two adjacent spiral grooves. Since the force of the first column acting on the flat groove is perpendicular to the flat groove, the cam cannot drive the drive shaft to move in the reverse direction, thereby preventing the external force from accidentally moving the position of the cam and affecting the stability of the overall structure, thereby further improving the effect of freezing the sample;

[0023] 5. After placing the cryotube in the refrigerator and putting on the cover, the pressing plate contacts the tube cover of the cryotube to limit the up and down movement of the cryotube in the refrigerator, thereby improving the effect of fixing the cryotube; at the same time, the pressing plate will be driven to move relative to the cover and compress the second spring provided between the pressing plate and the cover. The rebound force generated by the compression of the second spring will be transmitted through the cryotube, and a sealing strip is provided between the extrusion push plate and the groove to further prevent the cold air in the freezing chamber from leaking out through the through hole, thereby improving the effect of freezing samples in the refrigerator; in addition, the sliding connection between the guide column and the L-shaped block guides the sliding of the pressing plate on the cover, and the contact between the protrusion and the L-shaped block prevents the pressing plate from detaching from the cover, thereby improving the efficiency of fixing the cryotube. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Attached Figure 1 It is a schematic diagram of the structure inside the refrigerator of the present invention.

[0025] Attached Figure 2 It is a structural schematic diagram of the push block of the present invention.

[0026] Attached Figure 3 It is a structural schematic diagram of the piston of the present invention.

[0027] Attached Figure 4 It is a structural schematic diagram of a slider of the present invention.

[0028] Attached Figure 5 It is a structural schematic diagram of the first guide column of the present invention.

[0029] Attached Figure 6 It is a schematic structural diagram of the cooperation between the first guide column and the spiral groove of the present invention.

[0030] Attached Figure 7 It is a structural schematic diagram of a turntable of the present invention.

[0031] Attached Figure 8 It is a structural schematic diagram of the guide column of the present invention.

[0032] Attached Fig. 9 It is a structural schematic diagram of the cam of the present invention.

[0033] Numbers shown in the accompanying drawings:

[0034] 1. Refrigerator; 2. Cryogenic tube; 3. Partition; 4. Cover; 5. Freezing chamber; 6. Tube body; 7. Tube cover; 8. Notch; 9. Through hole; 10. Protruding plate; 11. Storage chamber; 12. Piston; 13. Push rod; 14. Push plate; 15. Push block; 16. Groove; 17. Cam;

[0035] 18. slide groove; 19. slider; 20. first plane; 21. second plane; 22. arc surface; 23. reset block; 24. first spring; 25. limit groove; 26. inclined surface; 27. first guide column; 28. drive shaft; 29. ​​spiral groove; 30. flat groove; 31. first motor; 32. drive gear; 33. driven gear;

[0036] 34. second guide column; 35. turntable; 36. arc block; 37. column; 38. second motor; 39. temperature sensor;

[0037] 40. Sealing strip; 41. Pressure plate; 42. Guide column; 43. L-shaped block; 44. Protrusion; 45. Second spring. DETAILED DESCRIPTION

[0038] The present invention will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms fall within the scope limited by the application equally.

[0039] The present invention provides a cryopreservation container with adjustable internal air pressure, such as Figure 1 , Figure 2 and Figure 3 As shown, it includes a refrigerator 1 and a plurality of cryogenic tubes 2, wherein the refrigerator 1 is provided with a partition 3 and a cover plate 4, wherein a plurality of freezing chambers 5 are formed between the partition 3 and the cover plate 4 and the refrigerator 1, wherein a cold air inlet is provided in the freezing chamber 5 to reduce the temperature of the freezing chamber 5 to 80°C, thereby realizing the freezing of samples inside the cryogenic tubes 2; a plurality of the cryogenic tubes 2 are arranged in parallel in the freezing chamber 5, and different samples are placed in cryogenic tubes 2 arranged in different ways, and subsequently a plurality of push blocks 15 can be used to drive the corresponding push plates 14 to move, thereby changing the air pressure interval of the cryogenic tubes 2 storing different samples, thereby meeting the storage requirements of different samples, and performing The effect of freezing samples is further improved. The freezing tube 2 includes a tube body 6 and a tube cover 7. The partition 3 is provided with a plurality of notches 8 and through holes 9 connecting the notches 8 and the outside. The end of the tube body 6 is provided with a convex plate 10 in contact with the notch 8. The tube cover 7 is in contact with the cover plate 4, and the cover plate 4 is in contact with the tube cover 7. The convex plate 10 is in contact with the notch 8, so as to limit the movement of the freezing tube 2 in the refrigerator 1, so that the position of the piston 12 can be better adjusted later, and the internal air pressure of the freezing tube 2 is always maintained in a stable range, thereby reducing the occurrence of sample sublimation during the freezing process and improving the effect of freezing samples.

[0040] The convex plate 10 blocks the flow between the freezing chamber 5 and the through hole 9. The tube body 6 is provided with a storage chamber 11 connected to the through hole 9. After all the cryopreservation tubes 2 are placed in the refrigerator 1 in sequence, the cover plate 4 at the top of the refrigerator 1 is covered, so that the freezing chamber 5 forms a sealed chamber to prevent subsequent cold air from leaking out and affecting the effect of freezing samples. A piston 12 is slidably connected in the storage chamber 11, and a push rod 13 is provided on one side of the piston 12. The push rod 13 extends to the outside of the tube body 6 and is provided with a push plate 14. By moving the push plate 14, the corresponding piston 12 is driven to slide upward in the tube sleeve, thereby changing the volume of the storage chamber 11, adjusting the air pressure in the storage chamber 11, compensating for the influence of the external temperature change of the cryopreservation tube 2 on the internal air pressure of the storage chamber 11, so that the internal air pressure of the cryopreservation tube 2 is always maintained in a stable range, thereby reducing the occurrence of sample sublimation during the freezing process and improving the effect of freezing samples.

[0041] The refrigerator 1 is symmetrically connected to a push block 15 for vertical sliding, and a groove 16 is provided on the push block 15. The upper and lower sides of the push plate 14 are respectively in contact with the groove 16. The refrigerator 1 is rotatably connected to a cam 17 for driving the push block 15 to move horizontally. The piston 12 of the cryogenic tube 2 is moved to the bottom end of the tube body 6 in advance, and then the two push blocks 15 are driven to move horizontally by the cam 17. Since the push plates 14 on each cryogenic tube 2 are located on the same horizontal line at this time, several push plates 14 enter the groove 16 provided on the push block 15 at the same time. When the push block 15 is pushed to move horizontally on the refrigerator 1, several push plates 14 can be driven to move at the same time, and there is no need to move the push plates 14 one by one, thereby simplifying the operating steps of moving multiple pistons 12 and improving the efficiency of freezing samples.

[0042] Preferably, Figure 4 and Fig. 9As shown, the refrigerator 1 is provided with a slide groove 18, and a slider 19 that slides in contact with the slide groove 18 is provided on one side of the push block 15, which plays a guiding role for the push block 15 to slide in the refrigerator 1, prevents the push plate 14 from falling off the push block 15, improves the stability of the overall structure, and further improves the effect of freezing samples; the left and right sides of the cam 17 are respectively provided with a first plane 20, and the front and rear sides of the cam 17 are respectively provided with a second plane 21, the spacing between the two first planes 20 is smaller than the spacing between the two second planes 21, and an arc surface 22 is provided between the first plane 20 and the second plane 21, and the first plane 20, the arc surface 22 and the second plane 21 are contacted with one side of the slider 19 in turn, and drive the slider 19 to slide horizontally in the slide groove 18, and a reset block 23 that contacts the other side of the slider 19 is slidably connected in the slide groove 18, and specifically, the reset block 23 is provided with a slider that slides in contact with the slide groove 18 on both sides. A horizontal block is dynamically connected, and a vertical block slidably connected to the reset block is provided on one side of the slider to improve the stability of the overall structure; a first spring 24 is provided between the reset block 23 and the refrigerator 1, and by rotating the cam 17, the first spring 24 is used to generate a rebound force after being compressed to drive the reset block 23 to slide in the slide groove 18, so that the slider 19 contacts the first plane 20, the arc surface 22, and the second plane 21 respectively, thereby changing the position of the slider 19 in the slide groove 18, so that the push plates 14 provided on the several cryopreservation tubes 2 can smoothly enter the groove 16 provided on the push block 15, and according to the temperature change in the freezing chamber 5, the positions of several pistons 12 in the tube body 6 are controlled at the same time to compensate for the external temperature change of the cryopreservation tube 2 and the influence of the internal air pressure of the storage chamber 11, so that the internal air pressure of the cryopreservation tube 2 is always maintained in a stable range, thereby reducing the occurrence of sample sublimation during the freezing process and improving the effect of freezing samples.

[0043] Preferably, Figure 4 and Fig. 9As shown, the slider 19 is provided with a plurality of limit grooves 25, and the upper and lower sides of the cam 17 are respectively in contact with the limit grooves 25, and limit the slider 19 to slide vertically in the slide groove 18, so as to prevent the external force from accidentally driving the slider 19 to move up and down, causing the air pressure of the storage chamber 11 inside the cryopreservation tube 2 to change, thereby ensuring that the air pressure inside the cryopreservation tube 2 is always maintained in a stable range during the cryopreservation process, reducing the occurrence of sample sublimation during the cryopreservation process, and improving the effect of freezing the sample; the upper and lower sides of the arc surface 22 are respectively provided with inclined surfaces 26 in contact with the limit grooves 25, and drive the slider 19 to slide horizontally in the slide groove 18, so that the slider 19 can slide vertically in the slide groove 18, according to the temperature change in the freezing chamber 5, control the positions of the plurality of pistons 12 in the tube body 6, compensate for the influence of the external temperature change of the cryopreservation tube 2 on the internal air pressure of the storage chamber 11, so that the internal air pressure of the cryopreservation tube 2 is always maintained in a stable range, thereby reducing the occurrence of sample sublimation during the cryopreservation process, and improving the effect of freezing the sample.

[0044] Preferably, Figure 5 and Figure 6 As shown, a plurality of first guide posts 27 are provided on one side of the cam 17, and a drive shaft 28 is rotatably connected to the refrigerator 1. A plurality of spiral grooves 29 are spirally provided on the drive shaft 28, and a flat groove 30 is provided between two adjacent spiral grooves 29. The plurality of spiral grooves 29 contact with the first guide posts 27 in sequence, and drive the cam 17 to rotate on the refrigerator 1, cooperate with the reset block 23 and the first spring 24, so that the slider 19 slides in the slide groove 18, so that the slider 19 contacts with the first plane 20, the second plane 21 and the arc surface 22 of the cam 17 respectively, drives the push block 15 to move, so that the push plates 14 provided on the plurality of cryogenic tubes 2 enter the groove 16, and improves the push block 15 and the plurality of cryogenic tubes. 2, and at the same time change the contact surface between the cam 17 and the slider 19, thereby changing the sliding state of the slider 19 in the slide groove 18, so as to adjust the internal air pressure of the cryopreservation tube 2 so that it is always maintained in a stable range, thereby reducing the occurrence of sample sublimation during the freezing process and improving the effect of freezing the sample; the flat groove 30 is in contact with the first guide column 27, and the force of the first column 37 on the flat groove 30 is perpendicular to the flat groove 30, so that the cam 17 cannot drive the drive shaft 28 to move in the reverse direction, thereby limiting the rotation of the cam 17 on the refrigerator 1, avoiding the external force from accidentally moving the position of the cam 17, affecting the stability of the overall structure, and further improving the effect of freezing the sample.

[0045] Preferably, Figure 5As shown, the refrigerator 1 is provided with a first motor 31, and the movable end of the first motor 31 is provided with a driving gear 32. The end of the driving shaft 28 is provided with a driven gear 33 meshing with the driving gear 32. The driving gear 32 is driven to rotate by the first motor 31, and the torque is transmitted through the driven gear 33 to drive the driving shaft 28 to rotate on the refrigerator 1, providing power for the rotation of the cam 17. At the same time, the motor serves as a driving power to better control the stop position of the cam 17, improve the stability of the overall structure, and further improve the effect of freezing samples.

[0046] Preferably, Figure 4 and Figure 7 As shown, a plurality of second guide posts 34 are provided on one side of the slider 19, and a turntable 35 is rotatably connected to the refrigerator 1, and an arc block 36 and a column 37 are provided on the turntable 35. The arc block 36 contacts with a plurality of second guide posts 34 at the same time, and the generated resistance will limit the slider 19 from sliding vertically in the slide groove 18, so as to prevent the external force from accidentally driving the slider 19 to move up and down, causing the air pressure of the storage chamber 11 inside the cryopreservation tube 2 to change, thereby ensuring that the air pressure inside the cryopreservation tube 2 is always maintained in a stable range during the cryopreservation process, reducing the occurrence of sample sublimation during the cryopreservation process, and improving the effect of freezing the sample; the column 37 contacts with a plurality of second guide posts 34 in turn, and drives the slider 19 to slide vertically in the slide groove 18, according to the temperature in the freezing chamber 5 The turntable 35 is rotated to make the upright posts 37 contact with the second guide posts 34 in turn, and drive the slider 19 to slide vertically in the slide groove 18, so as to control the positions of the pistons 12 in the tube body 6, compensate for the influence of the external temperature change of the cryogenic tube 2 on the internal air pressure of the storage chamber 11, so that the internal air pressure of the cryogenic tube 2 is always maintained in a stable range, thereby reducing the occurrence of sample sublimation during the freezing process and improving the effect of freezing the samples. In addition, by setting the height of the second guide post 34, the upright post 37 and the arc block 36, it is ensured that when the cam 17 drives the slider 19 to slide horizontally in the slide groove 18, the second guide post 34 is always in contact with the upright post 37 or the arc block 36, further ensuring the stability of the overall structure and improving the effect of freezing the samples.

[0047] Preferably, Figure 4 and Figure 7 As shown, the refrigerator 1 is provided with a second motor 38, and the movable end of the second motor 38 is connected to the turntable 35 to provide power for the turntable 35 to rotate in the refrigerator 1, while better controlling the vertical sliding distance of the slider 19 in the slide groove 18, ensuring that the internal air pressure of the cryogenic tube 2 is always maintained in a stable range during the freezing process, reducing the occurrence of sample sublimation during the freezing process, and improving the effect of freezing samples.

[0048] Preferably, Figure 2As shown, the refrigerator 1 is provided with a temperature sensor 39. The data monitored by the temperature sensor 39 is fed back to the control center, and the opening and closing of the first motor 31 and the second motor 38 are controlled, so as to adjust the air pressure in the storage chamber 11 according to the temperature change in the freezing chamber 5, so that the air pressure inside the cryogenic tube 2 is always maintained in a stable range, reducing the occurrence of sample sublimation during the freezing process and improving the effect of freezing samples. For details, please refer to the temperature sensor model CWDZ00.

[0049] Preferably, Figure 3 and Figure 8 As shown, a sealing strip 40 is provided between the push plate 14 and the groove 16 to prevent the cold air in the freezing chamber 5 from leaking out through the through hole 9 and affecting the freezing effect of the refrigerator 1. The cover plate 4 is slidably connected with a pressure plate 41 in contact with the tube cover 7. The pressure plate 41 is provided with a plurality of guide columns 42. The cover plate 4 is provided with a plurality of L-shaped blocks 43 slidably connected with the guide columns 42. The end of the guide column 42 is provided with a protrusion 44 in contact with the L-shaped block 43. A second spring 45 is provided between the guide column 42 and the cover plate 4. After the cryopreservation tube 2 is placed in the refrigerator 1 and the cover plate 4 is closed, the pressure plate 41 contacts the tube cover 7 of the cryopreservation tube 2 to limit the cryopreservation tube 2 from being moved upward in the refrigerator 1. The pressing plate 41 is moved downward to improve the effect of fixing the cryopreservation tube 2; at the same time, it will drive the pressing plate 41 to move relative to the cover plate 4 and compress the second spring 45 provided between the pressing plate 41 and the cover plate 4. The rebound force generated by the compression of the second spring 45 will be transmitted through the cryopreservation tube 2. A sealing strip 40 is provided between the extrusion push plate 14 and the groove 16 to further block the cold air in the freezing chamber 5 from leaking out through the through hole 9, thereby improving the effect of freezing samples in the refrigerator 1; in addition, the sliding connection between the guide column 42 and the L-shaped block 43 guides the sliding of the pressing plate 41 on the cover plate 4. At the same time, the contact between the protrusion 44 and the L-shaped block 43 prevents the pressing plate 41 from detaching from the cover plate 4, thereby improving the efficiency of fixing the cryopreservation tube 2.

[0050] Example 1

[0051] The present invention provides a cryopreservation container with adjustable internal air pressure, such as Figure 1 , Figure 2 and Figure 3As shown, the piston 12 of the cryopreservation tube 2 is moved to the bottom of the tube body 6 in advance, the tube cover 7 is opened to put the collected sample into the cryopreservation tube 2, and then the tube cover 7 is covered to put the cryopreservation tube 2 into the refrigerator 1, and the push rod 13 and the push plate 14 are respectively passed through the through holes 9 provided on the partition 3, so that the convex plate 10 contacts the notch 8. After the above actions are repeated in sequence to put all the cryopreservation tubes 2 into the refrigerator 1, the cover plate 4 at the top of the refrigerator 1 is covered to form a closed chamber in the freezing chamber 5 to prevent subsequent cold air from leaking out and affecting the effect of freezing the sample. At the same time, the cover plate 4 contacts the tube cover 7 to limit the movement of the cryopreservation tube 2 in the refrigerator 1, so that the position of the piston 12 can be better adjusted later to ensure that the internal air pressure of the cryopreservation tube 2 is always maintained in a stable range, thereby reducing the occurrence of sample sublimation during the freezing process and improving the effect of freezing the sample.

[0052] Then, the two push blocks 15 are driven to move horizontally by the cam 17. Since the push plates 14 on each cryogenic tube 2 are located on the same horizontal line at this time, several push plates 14 enter the grooves 16 provided on the push blocks 15 at the same time, and then gradually input cold air into the freezing chamber 5. At the same time, according to the temperature change in the freezing chamber 5, the push blocks 15 are gradually moved vertically. Since the upper and lower sides of several push plates 14 are in contact with the grooves 16 provided on the push blocks 15 at the same time, power is transmitted through the corresponding push rods 13, driving the corresponding pistons 12 to slide upward in the tube sleeve, thereby changing the volume of the storage chamber 11, adjusting the air pressure in the storage chamber 11, compensating for the influence of the external temperature change of the cryogenic tube 2 on the internal air pressure of the storage chamber 11, so that the internal air pressure of the cryogenic tube 2 is always maintained in a stable range, thereby reducing the occurrence of sample sublimation during the freezing process and improving the effect of freezing samples;

[0053] In addition, several push plates 14 can be driven to move at the same time by the push block 15, without moving the push plates 14 one by one, thereby simplifying the operating steps of moving multiple pistons 12 and improving the efficiency of freezing samples; in addition, since different samples have different stabilities under the same air pressure, the corresponding push plates 14 can be driven to move by several push blocks 15 to change the air pressure range of the freezing tubes 2 storing different samples, thereby meeting the storage requirements of different samples and further improving the effect of freezing samples.

[0054] Example 2

[0055] On the basis of Example 1, Figure 4 , Figure 5 , Figure 6 , Figure 7 and Fig. 9As shown, before placing the cryotube 2, the second plane 21 of the cam 17 contacts the slider 19, and the two push blocks 15 are located at the outermost side. After all the cryotubes 2 containing samples are placed in the refrigerator 1, the first motor 31 drives the driving gear 32 to rotate, and the torque is transmitted through the driven gear 33, driving the driving shaft 28 to rotate on the refrigerator 1, and contacts the corresponding first guide pillars 27 in sequence through a plurality of spiral grooves 29. The generated component force drives the cam 17 to rotate on the refrigerator 1, so that the contact surface between the cam 17 and the slider 19 gradually switches from the second plane 21 to the arc surface 22. At the same time, under the action of the rebound force of the first spring 24, the reset block 23 is driven to slide in the slide groove 18, and drives the two sliders 19 to gradually move inward until the push plates 14 provided on the plurality of cryotubes 2 enter the grooves 16 provided on the corresponding push blocks 15.

[0056] Then, the second motor 38 drives the turntable 35 to rotate, so that the arc block 36 provided on the turntable 35 slides out from between the plurality of second guide posts 34, and the restriction on the vertical sliding of the slider 19 in the slide slot 18 is released. Then, the column 37 contacts the second guide post 34, and the component force generated drives the slider 19 to slide vertically in the slide slot 18. Since the arc surface 22 of the cam 17 contacts the slider 19 at this time, and the inclined surfaces 26 provided on the upper and lower sides of the arc surface 22 contact the limit groove 25, the component force generated drives the slider 19 to move toward both sides and compresses the first spring 24, so that the slider 19 can move relative to the cam 17. Then, the thrust is transmitted through the push block 15, the push plate 14 and the push rod 13, and at the same time, the push block 15, the push plate 14 and the push rod 13 are driven to move relative to the cam 17. A plurality of corresponding pistons 12 are moved to slide on the tube body 6 to change the air pressure range of the cryopreservation tubes 2 storing different samples, thereby meeting the storage requirements of different samples and further improving the effect of freezing samples; then the temperature in the freezing chamber 5 is gradually reduced, and according to the temperature change in the freezing chamber 5, the corresponding push block 15 is driven vertically by the second motor 38 to move, thereby changing the volume of the storage chamber 11 of the corresponding cryopreservation tube 2, adjusting the air pressure in the storage chamber 11, compensating for the influence of the external temperature change of the cryopreservation tube 2 on the internal air pressure of the storage chamber 11, so that the internal air pressure of the cryopreservation tube 2 is always maintained in a stable range, thereby reducing the occurrence of sample sublimation during the freezing process and improving the effect of freezing samples;

[0057] After the temperature of the freezing chamber is constant, the cam 17 is further driven to rotate by the first motor 31 until the first plane 20 contacts the slider 19. At this time, when the external force drives the slider 19 to move vertically up and down, since the contact surfaces of the upper and lower sides of the cam 17 and the limit groove 25 are parallel to each other, no component force can be generated to drive the slider 19 to move toward both sides. The generated resistance will limit the movement of the slider 19 relative to the cam 17, thereby preventing the external force from accidentally driving the slider 19 to move up and down, causing the air pressure of the storage chamber 11 inside the freezing tube 2 to change, thereby ensuring that the air pressure inside the freezing tube 2 is always maintained in a stable range during the freezing process, reducing the occurrence of sample sublimation during the freezing process, and improving the effect of freezing samples;

[0058] In addition, during the freezing process, the arc block 36 will contact the plurality of second guide pillars 34 again, and the resistance generated after the contact between the two will further limit the vertical sliding of the slider 19 in the slide groove 18, thereby improving the effect of freezing the samples; at the same time, the first pillar 37 will also move to the flat groove 30 provided between two adjacent spiral grooves 29. Since the force exerted by the first pillar 37 on the flat groove 30 is perpendicular to the flat groove 30, the cam 17 cannot drive the drive shaft 28 to move in the reverse direction, thereby preventing the external force from accidentally moving the position of the cam 17 and affecting the stability of the overall structure, thereby further improving the effect of freezing the samples.

[0059] Example 3

[0060] On the basis of Example 1, Figure 3 and Figure 8 As shown, after the cryopreservation tube 2 is placed in the refrigerator 1 and the cover plate 4 is put on, the pressing plate 41 contacts the tube cover 7 of the cryopreservation tube 2 to limit the up and down movement of the cryopreservation tube 2 in the refrigerator 1, thereby improving the effect of fixing the cryopreservation tube 2; at the same time, the pressing plate 41 will be driven to move relative to the cover plate 4, and the second spring 45 provided between the pressing plate 41 and the cover plate 4 will be compressed. The rebound force generated by the compression of the second spring 45 will be transmitted through the cryopreservation tube 2, and a sealing strip 40 is provided between the extrusion push plate 14 and the groove 16 to further block the cold air in the freezing chamber 5 from leaking out through the through hole 9, thereby improving the effect of freezing samples in the refrigerator 1; in addition, the sliding connection between the guide column 42 and the L-shaped block 43 plays a guiding role in the sliding of the pressing plate 41 on the cover plate 4, and at the same time, the contact between the protrusion 44 and the L-shaped block 43 prevents the pressing plate 41 from detaching from the cover plate 4, thereby improving the efficiency of fixing the cryopreservation tube 2.

Claims

1. A freezing container with adjustable internal air pressure, comprising a refrigerator (1) and a plurality of freezing tubes (2), wherein the refrigerator (1) is provided with a partition (3) and a cover (4), wherein a plurality of freezing chambers (5) are formed between the partition (3) and the cover (4) and the refrigerator (1), wherein the plurality of freezing tubes (2) are arranged in parallel in the freezing chambers (5), wherein the freezing tubes (2) comprise a tube body (6) and a tube cover (7), wherein: The partition (3) is provided with a plurality of notches (8) and through holes (9) connecting the notches (8) and the outside world; the end of the tube body (6) is provided with a convex plate (10) in contact with the notch (8); the tube cover (7) is in contact with the cover plate (4); the convex plate (10) blocks the flow between the freezing chamber (5) and the through hole (9); the tube body (6) is provided with a storage chamber (11) in communication with the through hole (9); a piston (12) is slidably connected in the storage chamber (11); a push rod (13) is provided on one side of the piston (12); the push rod (13) extends to the outside of the tube body (6) and is provided with a push plate (14); The refrigerator (1) is symmetrically connected to a push block (15) for vertical sliding, and the push block (15) is provided with a groove (16). The upper and lower sides of the push plate (14) are respectively in contact with the groove (16). The refrigerator (1) is rotatably connected to a cam (17) for driving the push block (15) to move horizontally.

2. A cryopreservation container with adjustable internal air pressure according to claim 1, characterized in that: The refrigerator (1) is provided with a slide groove (18), one side of the push block (15) is provided with a slider (19) which is in sliding contact with the slide groove (18), the left and right sides of the cam (17) are respectively provided with first planes (20), the front and rear sides of the cam (17) are respectively provided with second planes (21), the spacing between the two first planes (20) is smaller than the spacing between the two second planes (21), an arc surface (22) is provided between the first plane (20) and the second plane (21), the first plane (20), the arc surface (22) and the second plane (21) are in contact with one side of the slider (19) in turn, and drive the slider (19) to slide horizontally in the slide groove (18), a reset block (23) which is in sliding connection with the other side of the slider (19) is provided in the slide groove (18), and a first spring (24) is provided between the reset block (23) and the refrigerator (1).

3. A cryopreservation container with adjustable internal air pressure according to claim 2, characterized in that: The slider (19) is provided with a plurality of limit grooves (25), the upper and lower sides of the cam (17) are respectively in contact with the limit grooves (25), and limit the slider (19) from sliding vertically in the slide groove (18), and the upper and lower sides of the arc surface (22) are respectively provided with inclined surfaces (26) in contact with the limit grooves (25), and drive the slider (19) to slide horizontally in the slide groove (18).

4. A cryopreservation container with adjustable internal air pressure according to claim 1, characterized in that: A plurality of first guide posts (27) are provided on one side of the cam (17), and a driving shaft (28) is rotatably connected to the refrigerator (1). A plurality of spiral grooves (29) are spirally provided on the driving shaft (28), and a flat groove (30) is provided between two adjacent spiral grooves (29). The plurality of spiral grooves (29) contact with the first guide posts (27) in sequence and drive the cam (17) to rotate on the refrigerator (1). The flat groove (30) contacts with the first guide posts (27) and limits the cam (17) from rotating on the refrigerator (1).

5. A cryopreservation container with adjustable internal air pressure according to claim 4, characterized in that: The refrigerator (1) is provided with a first motor (31), a driving gear (32) is provided at the movable end of the first motor (31), and a driven gear (33) meshing with the driving gear (32) is provided at the end of the driving shaft (28).

6. A cryopreservation container with adjustable internal air pressure according to claim 2, characterized in that: A plurality of second guide pillars (34) are provided on one side of the slider (19); a turntable (35) is rotatably connected to the refrigerator (1); an arc block (36) and a column (37) are provided on the turntable (35); the arc block (36) is in contact with the plurality of second guide pillars (37) at the same time, and limits the slider (19) from sliding vertically in the slide groove (18); the column (37) is in contact with the plurality of second guide pillars (34) in turn, and drives the slider (19) to slide vertically in the slide groove (18).

7. A cryopreservation container with adjustable internal air pressure according to claim 6, characterized in that: The refrigerator (1) is provided with a second motor (38), and the movable end of the second motor (38) is connected to the turntable (35).

8. The cryopreservation container with adjustable internal air pressure according to claim 1, characterized in that: A temperature sensor (39) is provided in the refrigerator (1).

9. The cryopreservation container with adjustable internal air pressure according to claim 1, characterized in that: A sealing strip (40) is provided between the push plate (14) and the groove (16); a pressure plate (41) in contact with the pipe cover (7) is slidably connected to the cover plate (4); a plurality of guide columns (42) are provided on the pressure plate (41); a plurality of L-shaped blocks (43) slidably connected with the guide columns (42) are provided on the cover plate (4); a protrusion (44) in contact with the L-shaped block (43) is provided at the end of the guide column (42); and a second spring (45) is provided between the guide column (42) and the cover plate (4).

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