Plasticizing liquid for algae plants and application of plasticizing liquid in preparation of algae plant specimens

By soaking and drying the algae plants with a plasticizing solution containing polyethylene glycol, methanol, glycerol and copper acetate, the problems of unstable color of algae plant specimens and formaldehyde in the preservation solution in the existing methods are solved, and the effect of long-term preservation of the morphology and color of algae plants is achieved.

CN119969386APending Publication Date: 2025-05-13DALIAN HOFFEN BIO TECHN
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Patent Information

Application Number
CN202510139180.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-08
Publication Date
2025-05-13

AI Technical Summary

Technical Problem

Among the existing methods used to make algae plant specimens, the compression method and the plasticization method cannot maintain the primary color of the plant for a long time, and the preservative solution used in the soaking method contains the volatile toxic substance formaldehyde.

Method used

A plasticizing solution for algae plants is provided, containing polyethylene glycol, methanol, glycerin and saturated copper acetate acetic acid solution as a color retention solution, and the specimens are prepared by soaking and drying.

Benefits of technology

This method can preserve the shape and color of algae plants for a long time. The specimens are not prone to deterioration, complete shape, good softness, not easy to damage, and not easy to relapse. They are suitable for teaching and research, specimen display, display and artistic viewing.

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Abstract

The invention provides a plasticizing liquid for algae and application of the plasticizing liquid in preparation of an algae specimen, and relates to the technical field of specimen preparation. The plasticizing liquid for algae is prepared from the following raw materials in parts by mass: 50 to 55 parts of polyethylene glycol, 35 to 40 parts of methanol, 5 to 10 parts of glycerol and 10 to 15 parts of color retention liquid. A specimen treated by adopting the plasticizing liquid disclosed by the invention is not easy to deteriorate, complete in shape, extremely good in softness, not easy to damage and not easy to get damp, and can be stored in air for a long time; the device not only plays an important role in teaching and scientific research, but also has an important value in the aspects of specimen display, exhibition, artistic appreciation and the like.
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Description

Technical Field

[0001] The invention relates to the technical field of specimen preparation, in particular to a plasticizing liquid for algae plants and application of the plasticizing liquid in preparing algae plant specimens. Background Art

[0002] At present, green plants are mostly preserved by pressing, traditional immersion, plastination and other plant specimen preparation methods. Among them, pressed specimens are easy to fall off and fade, have a single shape and are extremely easy to damage; the traditional immersion method specimens retain color close to the original color, but its preservation solution containing formaldehyde has defects such as volatility, toxicity, and irritating odor; plasticized specimens prepared by the plastination method are not easy to deteriorate, have diverse forms and are pollution-free, and are very popular, but they have the defects of poor color retention and color retention time, and contain a certain amount of formaldehyde, which is toxic. Therefore, a specimen preparation method that can preserve plant morphology and color for a long time is disclosed, which has important value in teaching and scientific research, specimen display, exhibition and artistic appreciation. Summary of the invention

[0003] The purpose of the present invention is to provide a plasticizing liquid for algae plants and its application in preparing algae plant specimens, so as to solve the problem that the pressing method and the plasticizing method in the existing methods for preparing algae plant specimens cannot keep the original color of the plants for a long time, and the problem that the preservation liquid obtained by the immersion method contains the volatile toxic substance formaldehyde.

[0004] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0005] The present invention provides a plasticizing liquid for algae plants, comprising the following raw materials in parts by weight:

[0006] 50-55 parts of polyethylene glycol, 35-40 parts of methanol, 5-10 parts of glycerol, 10-15 parts of color retaining solution;

[0007] The color preserving solution is a saturated copper acetate acetic acid solution.

[0008] Preferably, the molecular weight of the polyethylene glycol is 400.

[0009] Preferably, the color preserving solution is prepared by adding copper acetate to a 50% acetic acid solution until saturated to obtain the color preserving solution.

[0010] The present invention also provides an application of the above-mentioned plasticizing liquid for algae in preparing algae specimens, comprising the following steps:

[0011] 1) The algae samples are sequentially cleaned, disinfected with alcohol, washed with water, and then immersed in a plasticizing solution for algae;

[0012] 2) After the soaking is completed, the algae sample is taken out and dried to obtain an algae specimen.

[0013] Preferably, the soaking time in step 1) is 48 to 72 hours.

[0014] Preferably, the drying temperature in step 2) is 20-28° C. and the drying time is 10-15 days.

[0015] The present invention has at least the following beneficial effects:

[0016] This application changes the original configuration of the plant plasticizing preservation solution. The polyethylene glycol in the plasticizing solution acts as a solvent and softens, methanol plays a fixing role, glycerol cooperates with the polyethylene glycol to soften, and the color preserving solution preserves the color. During use, the cell fluid in the algae plant cells is replaced with the preservation solution by soaking. The specimens after plasticizing are not easy to deteriorate, have complete morphology, excellent softness, are not easy to be damaged, are not easy to get damp, and can be placed in the air for a long time. This not only plays an important role in teaching and scientific research, but also has important value in specimen display, exhibition and artistic appreciation. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a real picture of the algae specimen prepared in Example 1 of the present invention after being placed for 10 days;

[0018] Figure 2 This is a real picture of the algae plant specimen prepared in Example 1 of the present invention after being placed for 2 months;

[0019] Figure 3 This is a real picture of the algae specimen prepared in Example 1 of the present invention after being placed for 6 months;

[0020] Figure 4 This is a physical picture of the algae specimen prepared in Example 2 of the present invention;

[0021] Figure 5 This is a physical picture of the algae specimen prepared in Example 3 of the present invention;

[0022] Figure 6 This is a physical picture of the algae specimen prepared in Example 4 of the present invention. DETAILED DESCRIPTION

[0023] The present invention provides a plasticizing liquid for algae plants, comprising the following raw materials in parts by weight:

[0024] 50-55 parts of polyethylene glycol, 35-40 parts of methanol, 5-10 parts of glycerol, 10-15 parts of color preserving solution, preferably 51-54 parts of polyethylene glycol, 36-39 parts of methanol, 6-9 parts of glycerol, 11-14 parts of color preserving solution, more preferably 52-53 parts of polyethylene glycol, 37-38 parts of methanol, 7-8 parts of glycerol, 12-13 parts of color preserving solution;

[0025] The color preserving solution is a saturated copper acetate acetic acid solution.

[0026] Preferably, the molecular weight of the polyethylene glycol is 400.

[0027] Preferably, the color preserving solution is prepared by adding copper acetate to a 50% acetic acid solution until saturated to obtain the color preserving solution.

[0028] The present invention also provides an application of the above-mentioned plasticizing liquid for algae in preparing algae specimens, comprising the following steps:

[0029] 1) The algae samples are sequentially cleaned, disinfected with alcohol, washed with water, and then immersed in a plasticizing solution for algae;

[0030] 2) After the soaking is completed, the algae sample is taken out and dried to obtain an algae specimen.

[0031] Preferably, the algae plant may be sea lettuce, large-leaf seaweed, small-leaf seaweed or small algae.

[0032] Preferably, the soaking time in step 1) is 48 to 72 hours, preferably 52 to 68 hours, more preferably 55 to 65 hours, and more preferably 58 to 62 hours.

[0033] Preferably, the drying temperature in step 2) is 20-28°C, preferably 21-26°C, and more preferably 22-24°C; the drying time is 10-15 days, which can be 10 days, 11 days, 12 days, 13 days, 14 days or 15 days.

[0034] Preferably, the algae plant samples need to be pruned before cleaning to remove rotten leaves, broken leaves, yellow leaves, diseased leaves and some twigs to avoid overlapping of sample tissues.

[0035] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0036] Example 1

[0037] Add copper acetate crystals gradually into 50% acetic acid solution, stirring continuously until saturated to obtain a color-preserving solution;

[0038] Mixing polyethylene glycol 400, methanol, glycerol and color preservative solution in a mass ratio of 50:35:5:10 to obtain a plasticizing solution for algae;

[0039] The mud and sand on the sea lettuce were washed off, and the samples were soaked in 70% ethanol for 5 minutes for disinfection. After the samples were rinsed with distilled water, they were soaked in distilled water for 15 minutes, and then rinsed with distilled water for 3 times. Finally, the surface of the samples was wiped clean, and the samples were placed in the above-mentioned plasticizing liquid for algae and soaked for 72 hours. During the soaking process, the color of the plants approached the original color of the plants. Then, the samples were taken out and placed in a ventilated and cool place (25°C) to dry in the shade for 15 days to obtain algae specimens. Figures 1 to 3 Actual pictures of the plant specimens prepared in this example after being placed for 10 days, 2 months and 6 months respectively.

[0040] Example 2

[0041] Add copper acetate crystals gradually into 50% acetic acid solution, stirring continuously until saturated to obtain a color-preserving solution;

[0042] Mixing polyethylene glycol 400, methanol, glycerol and color preservative solution in a mass ratio of 55:38:7:14 to obtain a plasticizing solution for algae;

[0043] The mud and sand on the large-leaf seaweed were washed off, and the samples were soaked in 70% ethanol for 5 minutes for disinfection. After the samples were rinsed with distilled water, they were soaked in distilled water for 15 minutes, and then rinsed with distilled water for 3 times. Finally, the surface of the samples was wiped clean, and the samples were placed in the above-mentioned plasticizing liquid for algae and soaked for 62 hours. During the soaking process, the color of the plants approached the original color of the plants. Then, the samples were taken out and placed in a ventilated and cool place (25°C) to dry in the shade for 14 days to obtain algae specimens. The actual specimens prepared in this embodiment are shown in the figure. Figure 4 shown.

[0044] Example 3

[0045] Add copper acetate crystals gradually into 50% acetic acid solution, stirring continuously until saturated to obtain a color-preserving solution;

[0046] Mixing polyethylene glycol 400, methanol, glycerol and color preservative solution in a mass ratio of 53:40:10:12 to obtain a plasticizing solution for algae;

[0047] The mud and sand on the small algae were washed off, and the samples were soaked in 70% ethanol for 5 minutes for disinfection. After the disinfected samples were rinsed with distilled water, they were soaked in distilled water for 15 minutes, and then the samples were rinsed with distilled water for 3 times. Finally, the surface of the samples was wiped clean, and the samples were placed in the above-mentioned plasticizing liquid for algae and soaked for 65 hours. During the soaking process, the color of the plants approached the original color of the plants. Then, the samples were taken out and placed in a ventilated and cool place (28°C) to dry in the shade for 13 days to obtain algae specimens. The actual specimens prepared in this embodiment are shown in the figure. Figure 5 shown.

[0048] Example 4

[0049] Add copper acetate crystals gradually into 50% acetic acid solution, stirring continuously until saturated to obtain a color-preserving solution;

[0050] Mixing polyethylene glycol 400, methanol, glycerol and color preservative solution in a mass ratio of 54:37:9:13 to obtain a plasticizing solution for algae;

[0051] The mud and sand on the small-leaved seaweed were washed off, and the samples were soaked in 70% ethanol for 5 minutes for disinfection. After the samples were rinsed with distilled water, they were soaked in distilled water for 15 minutes, and then rinsed with distilled water for 3 times. Finally, the surface of the samples was wiped clean, and the samples were placed in the above-mentioned plasticizing liquid for algae and soaked for 48 hours. During the soaking process, the color of the plants approached the original color of the plants. Then, the samples were taken out and placed in a ventilated and cool place (22°C) to dry in the shade for 15 days to obtain algae specimens. The actual specimens prepared in this embodiment are shown in the figure below. Figure 6 shown.

[0052] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A plasticizing liquid for algae, characterized in that: Contains the following raw materials in parts by weight: 50-55 parts of polyethylene glycol, 35-40 parts of methanol, 5-10 parts of glycerol, 10-15 parts of color retaining solution; The color preserving solution is a saturated copper acetate acetic acid solution.

2. The plasticizing liquid for algae according to claim 1, characterized in that: The molecular weight of the polyethylene glycol is 400.

3. The plasticizing liquid for algae according to claim 1, characterized in that: The preparation method of the color preserving solution is as follows: copper acetate is added into a 50% acetic acid solution until the solution is saturated to obtain the color preserving solution.

4. Use of the plasticizing liquid for algae according to any one of claims 1 to 3 in making algae specimens, characterized in that: The following steps are involved: 1) The algae samples are sequentially cleaned, disinfected with alcohol, washed with water, and then immersed in a plasticizing solution for algae; 2) After the soaking is completed, the algae sample is taken out and dried to obtain an algae specimen.

5. The use of a plasticizing liquid for algae plants according to claim 4 in preparing algae plant specimens, characterized in that: The soaking time in step 1) is 48 to 72 hours.

6. The use of a plasticizing liquid for algae plants according to claim 5 in preparing algae plant specimens, characterized in that: The drying temperature in step 2) is 20-28° C. and the drying time is 10-15 days.