A pesticide composition containing polyoxin and its application

Abstract

The present application belongs to the technical field of pesticide fungicide, and specifically discloses a pesticide composition containing polyoxin, which comprises active ingredient I and active ingredient II, wherein the active ingredient I is polyoxin B, the active ingredient II is any one of fluazinam or bifenazate, and the mass ratio of the active ingredient I to the active ingredient II is 1:48-50:1. The pesticide composition has a synergistic effect on various pathogenic bacteria, can effectively reduce the use dosage of the pesticide, and is safe to crops and other non-target organisms.

Description

[0001] The present application is a divisional application of application number CN202311225752.3, filed on September 22, 2023, entitled "Pesticide composition containing polyoxin and application thereof". TECHNICAL FIELD

[0002] The present application belongs to the technical field of pesticide fungicides, and specifically relates to a pesticide composition containing polyoxin and application thereof. BACKGROUND

[0003] Polyoxin B (polyoxin), also known as polyoxin, is a metabolic product produced by Streptomyces aureus, and is a peptide pyrimidine nucleoside agricultural antibiotic produced by modern biological engineering technology. It has systemic and therapeutic effects and high target biological selectivity. Its mechanism of action is to inhibit the synthesis of chitin in fungal cell walls. Its structure is similar to that of chitin synthase substrate uridine diphosphate glucosamine, and it belongs to a competitive inhibitor. Because it is a safe pesticide with high efficiency, low toxicity and no environmental pollution, it is widely used in the prevention and control of important diseases of food crops, fruits and vegetables, etc.

[0004] Sedaxane, ISO common name: sedaxane, CAS number: 874967-67-6, chemical name: N-[2-[1,1'-bicyclopropyl]-2-ylphenyl]-3-(difluoromethyl)-1-methyl-1H-pyrazole-carboxamide, its structure is divided into cis and trans structures, and the trans structure is mainly. Sedaxane is a succinate dehydrogenase inhibitor fungicide, which affects the mitochondrial respiratory electron transport system of pathogenic fungi by inhibiting protein complex II (succinate dehydrogenase) in the mitochondrial respiratory electron transport chain of pathogenic fungi, hinders its energy metabolism, and inhibits the growth of pathogenic fungi and causes them to die. It is suitable for crops such as cereals, soybeans, rapeseed, potatoes and sugarcane, and can be used for seed treatment to prevent and control smut, bunt, scab, gray mold and other diseases.

[0005] Bifenaptamid is a fungicide developed by Bayer, and its chemical name is N-(3',4'-dichloro-5-fluoro[1,1'-biphenyl]-2-yl)-3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carboxamide. Bifenaptamid belongs to succinate dehydrogenase inhibitors and works by inhibiting succinate dehydrogenase in mitochondrial respiration. It is mainly used in cotton, barley, sunflower, soybean, wheat, rapeseed and other crops to prevent and control diseases caused by ascomycetes, basidiomycetes and hemiascomycetes. Bifenaptamid has significant effect in preventing and treating leaf blight, rust and other diseases of cereal crops.

[0006] In agricultural production, the use of a single pesticide in large quantities leads to the development of target fungal resistance, greatly shortens the use of pesticides, causes the use of pesticides to increase year by year, and has a negative impact on the environment, which is not conducive to the sustainable and healthy development of agriculture. SUMMARY

[0007] The present application provides a pesticide composition containing polyoxin and its preparation, which has good control effect on various plant diseases, can effectively reduce the use of pesticides, reduce the cost of pesticides, is safe for crops, and reduces the pesticide residues of agricultural products.

[0008] In order to achieve the above purpose, the technical scheme adopted by the present application is as follows: a pesticide composition containing polyoxin B, the pesticide composition comprising active ingredient I and active ingredient II, the active ingredient I being polyoxin B, and the active ingredient II being any one of fluazinam or bifenazate;

[0009] Further, the active ingredient II is fluazinam, and the mass ratio of the active ingredient I to the active ingredient II is 1:30-10:1;

[0010] Further, the mass ratio of the active ingredient I to the active ingredient II is 1:20-10:1;

[0011] Further, the active ingredient II is bifenazate, and the mass ratio of the active ingredient I to the active ingredient II is 1:30-10:1;

[0012] Further, the mass ratio of the active ingredient I to the active ingredient II is 1:24-10:1;

[0013] Further, the total weight of the pesticide composition is 100wt%, and the total weight of the active ingredient I and the active ingredient II accounts for 1%-90% of the total weight of the pesticide composition;

[0014] Further, the total weight of the pesticide composition is 100wt%, and the total weight of the active ingredient I and the active ingredient II accounts for 2%-80% of the total weight of the pesticide composition;

[0015] Further, the pesticide composition further comprises an agriculturally permissible auxiliary ingredient in addition to the active ingredients, and the auxiliary ingredient is selected from one or more of wetting agents, dispersants, emulsifiers, thickening agents, disintegrating agents, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, synergists or carriers;

[0016] Further, the wetting agent is selected from a mixture consisting of one or more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, Laikan Powder BX, wetting penetrant F, soap nut powder, silkworm excrement, or soapberry powder;

[0017] Further, the dispersing agent is selected from a mixture consisting of one or more of polycarboxylate, lignin sulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, calcium alkyl benzene sulfonate, naphthalene sulfonic acid formaldehyde condensate sodium salt, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, fatty acid polyoxyethylene ether, or glycerol fatty acid ester polyoxyethylene ether;

[0018] Further, the emulsifying agent is selected from a mixture consisting of one or more of calcium alkyl benzene sulfonate, OP series phosphate ester (nonylphenol polyoxyethylene ether phosphate ester), phenylphenol polyoxyethylene ether phosphate ester, styrene polyoxyethylene ether ammonium sulfate salt, alkyl biphenyl ether disulfate magnesium salt, triethanolamine salt, benzyl dimethyl phenol polyoxyethylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethyl phenol formaldehyde resin polyoxyethylene ether, phenylethyl phenol polyoxyethylene polypropylene ether, ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), castor oil polyoxyethylene ether, alkyl aryl polyoxyethylene polyoxypropylene ether, sorbitan monostearate, sorbitan fatty acid ester polyoxyethylene ether, or fatty alcohol polyoxyethylene ether;

[0019] Further, the thickening agent is selected from a mixture consisting of one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose, or magnesium aluminum silicate;

[0020] Further, the disintegrating agent is selected from a mixture consisting of one or more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid, or sodium bicarbonate;

[0021] Further, the antifreezing agent is selected from a mixture consisting of one or more of ethylene glycol, propylene glycol, glycerol, or urea;

[0022] Further, the defoaming agent is selected from a mixture consisting of one or more of silicone oil, silicone compound, C 10 ~ C 20 saturated fatty acid compound, or C8~C 10 fatty alcohol compound;

[0023] Further, the solvent is selected from a mixture consisting of one or more of N,N-dimethylformamide, cyclohexanone, butyl ether, dimethylbenzene, dimethyl sulfoxide, methanol, ethylene glycol, ethanol, propanol, butanol, trimethylcyclohexanone, N-octyl pyrrolidone, toluene, ethanolamine, triethanolamine, isopropylamine, N-methyl pyrrolidone, diethylene glycol, ethylene glycol methyl ether, ethyl acetate, or acetonitrile;

[0024] Further, the stabilizer is selected from the group consisting of one or more of epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate, triphenyl phosphate;

[0025] Further, the penetrant is selected from the group consisting of one or more of penetrant JFC, penetrant T, azone or organosilicon;

[0026] Further, the carrier is one, two or three of a solvent or a filler, and the water is preferably deionized water;

[0027] Further, the filler is selected from the group consisting of one or more of kaolin, diatomite, bentonite, attapulgite, white carbon, starch or light calcium carbonate;

[0028] The above-mentioned substances are all commercially available;

[0029] The pesticide composition of the present application can be prepared into any one of the formulation forms allowed in agriculture;

[0030] Further, the formulation form is a solid formulation, a liquid formulation and / or a seed treatment formulation;

[0031] Further, the solid formulation is a direct use solid formulation, a dispersible solid formulation or a soluble solid formulation;

[0032] Still further, the direct use solid formulation is a powder, a granule, a pellet, a tablet or a strip;

[0033] The dispersible solid formulation is a wettable powder, an oil dispersible powder, a milk powder, a water dispersible granule, a milk granule or a water dispersible tablet;

[0034] The soluble solid formulation is a soluble powder, a soluble tablet or a soluble granule;

[0035] Further, the liquid formulation is a solution formulation, a dispersion liquid formulation, an emulsion formulation, a suspension formulation or a multi-phase formulation;

[0036] Still further, the solution formulation is a soluble liquid, a soluble gel, an oil or a spread film oil;

[0037] The dispersion liquid formulation is an emulsion, a latex, a dispersible liquid or a paste;

[0038] The emulsion formulation is a water emulsion, an oil emulsion, a microemulsion or a lipid;

[0039] The suspension formulation is a suspension, a microcapsule suspension, an oil suspension or a dispersible oil suspension;

[0040] The multi-phase preparation is a suspoemulsion, a microcapsule suspension-suspension, a microcapsule suspension-emulsion or a microcapsule suspension-suspoemulsion.

[0041] Further, the seed treatment preparation includes a seed treatment solid preparation or a seed treatment liquid preparation.

[0042] Further, the seed treatment solid preparation is a seed treatment dry powder or a seed treatment dispersible powder.

[0043] The seed treatment liquid preparation is a seed treatment liquid, a seed treatment emulsion and / or a seed treatment suspension.

[0044] Further, the preparation form is a solid preparation and / or a liquid preparation, wherein the solid preparation is and the liquid preparation is.

[0045] The present application also discloses the application of the pesticide composition and / or the preparation thereof as described above in the prevention and treatment of plant diseases.

[0046] Further, the plant is a cereal crop, the cereal crop is wheat, and the disease is wheat scab and wheat powdery mildew.

[0047] Further, the plant disease is caused by one or more pathogenic fungi from the phyla of Mastigomycota, Zygomy-cota, Ascomycota, Basidiomycota and / or Deuteromycota.

[0048] Further, the pesticide composition and / or the preparation thereof is applied to the pathogenic fungi in need of prevention and treatment or the medium where the pathogenic fungi grow in an effective dose.

[0049] The present application has the following advantages:

[0050] 1) The pesticide composition of the present application has a synergistic effect on multiple pathogenic fungi, can be used for preventing and treating multiple plant diseases, and expands the application range.

[0051] 2) The pesticide composition of the present application can effectively reduce the use dose of pesticides and reduce the pesticide residues of agricultural products.

[0052] 3) The pesticide composition of the present application can slow down the development of pathogenic fungi resistance and is safe to crops and other non-target organisms. DETAILED DESCRIPTION

[0053] In order to make the technical solutions, purposes and advantages of the present application clearer, the present application is described in the following specific embodiments, but the present application can be realized in various forms and should not be limited by the embodiments described herein.

[0054] Preparation examples

[0055] Preparation Example 1: 20% polyoxin B · fluazinam suspension concentrate (1:1)

[0056] By weight percentage, 10% polyoxin B, 10% fluazinam, 3% alkylphenol polyoxyethylene ether, 4% fatty alcohol polyoxyethylene ether phosphate, 1% polycarboxylic acid sodium salt, 1% magnesium aluminum silicate, 0.25% xanthan gum, 5% glycerol, 0.1% sodium benzoate, 0.5% silicone oil, deionized water to make up the balance.

[0057] Preparation method: according to the formula proportion, the active ingredients, surfactants and other functional additives are placed in the reaction kettle in turn, mixed with water, sheared by high speed, wet sand ground, and finally filtered to obtain the suspension concentrate product.

[0058] Preparation Example 2: 24% polyoxin B · bifenaptam suspension concentrate (1:3)

[0059] By weight percentage, 6% polyoxin B, 18% bifenaptam, 1% isomeric tridecanol polyoxyethylene ether, 2% naphthalene sulfonate formaldehyde condensate, 3% alkylphenol polyoxyethylene ether phosphate, 0.3% carboxymethyl cellulose, 2% magnesium aluminum silicate, 5% glycerol, 0.25% silicone oil, deionized water to make up the balance.

[0060] Preparation method: same as preparation example 1.

[0061] Preparation Example 3: 30% polyoxin B · fluazinam water dispersible granule (1:2)

[0062] By weight percentage, 10% polyoxin B, 20% fluazinam, 10% sodium lignosulfonate, 5% naphthalene sulfonate formaldehyde condensate, 2% sodium dodecyl sulfate 6% white carbon black, 25% starch, kaolin to make up the balance.

[0063] Preparation method: according to the formula proportion, the active ingredients are added to the carrier, and the surfactants and other functional additives are added therein, mixed, and then 10-25% water is added after air flow crushing, and then kneading, granulation, drying and screening to obtain the water dispersible granule product; or the crushed powder is sprayed with water in a fluidized bed granulator, granulated, dried, and then screened to obtain the product.

[0064] Preparation Example 4: 35% polyoxin B · bifenaptam water dispersible granule (1:6)

[0065] By weight percentage, 5% polyoxin B, 30% bifenaptam, 8% naphthalene sulfonate formaldehyde condensate, 6% sodium lignosulfonate, 5% Laka powder BX, 10% ammonium sulfate, kaolin to make up the balance.

[0066] Preparation method: same as preparation example 3.

[0067] Preparation Example 5: 30% polyoxin B · pydiflumetofen wettable powder (2:1)

[0068] By weight percentage, 20% polyoxin B, 10% pydiflumetofen, 8% sodium lignosulfonate, 7% alkyl naphthalene sulfonate formaldehyde condensate, 2% sodium dodecyl sulfate, 15% white carbon black, kaolin to make up the balance.

[0069] Preparation method: mix the active ingredients, dispersants, wetting agents and fillers according to the formulation ratio, uniformly stir in a stirred tank, and uniformly mix by air jet mill for multiple times to prepare the wettable powder of the present application.

[0070] Preparation Example 6: 42% polyoxin B · bifenazate wettable powder (1:5)

[0071] By weight percentage, 7% polyoxin B, 35% bifenazate, 7% naphthalene sulfonate formaldehyde condensate, 8% sodium lignosulfonate, 3% kaolin, and kaolin to make up the balance.

[0072] Preparation method: same as Preparation Example 5.

[0073] Indoor joint action test

[0074] Example 1: indoor joint action test of different pesticides on wheat scab

[0075] Test basis: the test refers to NY / T 1156.2-2006 "Agricultural indoor biological determination test guidelines fungicides part 2: inhibition of pathogenic fungal mycelial growth test plate method".

[0076] Test target: Fusarium graminearum.

[0077] Test instruments: wet heat sterilization pot, super clean bench, constant temperature light incubator, electric heating drum airproof drying oven, one-millionth electronic balance, pipette, alcohol lamp, small beaker, volumetric flask, flask, culture dish (Φ9cm), puncher, inoculator, ruler, etc.

[0078] Test target culture conditions: the Fusarium graminearum stored in a refrigerator at 4℃ indoors was transferred to potato dextrose agar medium and cultured in a 26℃ incubator in the dark for 4d to activate it for standby use.

[0079] Test pesticides: 32% polyoxin B technical material, 92% bifenazate technical material, 96% pydiflumetofen technical material.

[0080] Pesticide preparation: the above pesticides were respectively dissolved and diluted with appropriate solvents, different ratios were designed according to the purpose of mixing, the activity of the pesticides, and the single agent and each group of mixed agents were configured into the required series of mass concentrations.

[0081] Test repeats: 4 culture dishes for each concentration of test agent, 4 repeats in total, 1 culture dish for each repeat, and a treatment without agent as a blank control.

[0082] Agent treatment: Under sterile operating conditions, the sterilized medium was quantitatively added to a sterile conical flask according to the test treatment, and the agent liquid was quantitatively drawn from low concentration to high concentration and added to the conical flask, and shaken well. Then, an equal amount was poured into 4 culture dishes to make the corresponding concentration of agent-containing plates.

[0083] Inoculation: The cultured pathogenic bacteria were cut with a sterilized puncher with a diameter of 5 mm from the edge of the colony, and the bacterial cake was inoculated in the center of the agent-containing plate with the inoculator, with the mycelium facing up, and the dish cover was placed in the incubator at the appropriate temperature for culture.

[0084] Data investigation: According to the mycelial growth of the blank control culture dish, the mycelial growth of the pathogenic bacteria was investigated. The colony diameter was measured with a ruler, in centimeters (cm), and each colony was measured with a cross method once, and the average value was taken and the original data of all repeats of each treatment were recorded.

[0085] Data statistics and analysis: According to the investigation results, the mycelial growth inhibition rate of each treatment concentration on the test target bacteria was calculated, with the unit being percentage (%), and the calculation results were rounded to two decimal places.

[0086] D = D1 - D2

[0087] In the formula:

[0088] D - colony growth diameter;

[0089] D1 - colony diameter;

[0090] D2 - bacterial cake diameter.

[0091]

[0092] In the formula:

[0093] I - mycelial growth inhibition rate;

[0094] D0 - blank control colony growth diameter;

[0095] D T - agent-treated colony growth diameter.

[0096] The data was processed by the method of probability value analysis. The DPS statistical analysis system was used for analysis to obtain the virulence regression line, EC 50 value, and to evaluate the activity of the test agent on the biological test material.

[0097] Sun Yunpei method: According to the co-toxicity coefficient (CTC) to evaluate the synergistic effect of mixed drugs, the co-toxicity coefficient CTC of the compound is greater than or equal to 120, which shows synergistic effect; CTC is less than or equal to 80, which shows antagonistic effect; 80 < CTC < 120 shows additive effect.

[0098] The co-toxicity coefficient (CTC value) of the mixed agent is calculated as follows:

[0099]

[0100] In the formula:

[0101] ATI - the measured toxicity index of the mixed agent;

[0102] S - the EC of the standard drug, unit: milligrams per liter (mg / L); 50

[0103] M - the EC of the mixed agent, unit: milligrams per liter (mg / L). 50

[0104] TTI = TI A × P A + TI B × P B

[0105] In the formula:

[0106] TTI - the theoretical toxicity index of the mixed agent;

[0107] TI A - the toxicity index of A agent;

[0108] P A - the percentage content of A agent in the mixed agent, unit: percentage (%);

[0109] TI B - the toxicity index of B agent;

[0110] P B - the percentage content of B agent in the mixed agent, unit: percentage (%).

[0111]

[0112] In the formula:

[0113] CTC - co-toxicity coefficient;

[0114] ATI - the measured toxicity index of the mixed agent;

[0115] TTI - the theoretical toxicity index of the mixed agent.

[0116] The results of the indoor test are shown in the following table: ​​

[0117] Table 1 The indoor synergistic effect test results of polyoxin B and bifenpirim on wheat scab

[0118] Test agent and ratio Regression equation (Y=) EC 50 (mg / L) Co-toxicity coefficient Polyoxin B y=5.2243+1.4808x 0.7056 - Bixafen y=4.3903+1.2430x 3.0940 - Polyoxin B: Bixafen = 1:50 y=4.5740+1.0863x 2.4670 117.610 Polyoxin B: Bixafen = 1:30 y=4.6160+1.1643x 2.1372 130.518 Polyoxin B: Bixafen = 1:10 y=4.7864+1.0706x 1.5832 149.441 Polyoxin B: Bixafen = 1:5 y=4.9114+1.2495x 1.1774 168.003 Polyoxin B: Bixafen = 1:3 y=5.0899+1.2341x 0.8445 198.443 Polyoxin B: Bixafen = 1:1 y=5.1640+1.1224x 0.7143 160.876 Polyoxin B: Bixafen = 3:1 y=5.2358+1.1142x 0.6142 142.353 Polyoxin B: Bixafen = 5:1 y=5.2504+1.1184x 0.5972 135.597 Polyoxin B: Bixafen = 10:1 y=5.2240+0.9920x 0.5945 127.646 Polyoxin B: Bixafen = 30:1 y=5.2226+1.1255x 0.6341 114.118 Polyoxin B: Bixafen = 50:1 y=5.1992+1.0880x 0.6560 109.214

[0119] Table 2 The indoor synergistic effect test results of polyoxin B and fluazinam on wheat scab

[0120]

[0121]

[0122] The indoor test results of Table 1 and Table 2 show that the combination of polyoxin and any one of bifenpirim or fluazinam has obvious synergistic effect on wheat scab within a reasonable range.

[0123] Example 2: Indoor synergistic effect test of different treatment agents on wheat powdery mildew

[0124] Test basis: The test refers to NY / T 1156.4-2006 "Agricultural Indoor Biological Test Guidelines Pesticides Part 4: Prevention of Wheat Powdery Mildew Test Potting Method".

[0125] Test target: Blumeria graminis f. sp. tritici.

[0126] Test agents: Select a wheat variety Lu Yuan 502 susceptible to powdery mildew, and number the seedlings for standby when the seedlings grow to 2-3 leaf stage.

[0127] Test agents: 32% polyoxin B technical, 92% bifenpirim technical, and 96% fluazinam technical, which are provided by the R&D Center of Hailier Pharmaceutical Group.

[0128] Test preparation: Dissolve and dilute the above agents with appropriate solvents, design different ratios according to the purpose of mixing and the activity of the agents, and configure the single agent and each group of mixed agents into the required series of mass concentrations.

[0129] Inoculation: Fresh spores of powdery mildew produced within 24 hours on diseased wheat leaves were evenly shaken and inoculated on the treated 2-3 leaf stage potting wheat seedlings. Four pots were used for each treatment, with 10 plants per pot.

[0130] Agent treatment: The agents were evenly sprayed on the standby wheat seedlings using the spray method, and then naturally air-dried. A treatment without agents was set as a blank control. After application, the test was cultured under suitable conditions.

[0131] Data investigation: The incidence was investigated according to the classification of the blank control. The following classification method was used:

[0132] 0 grade: no disease spot;

[0133] 1 grade: disease spot area accounts for less than 5% of whole leaf area;

[0134] 3 grade: disease spot area accounts for 6-15% of whole leaf area;

[0135] 5 grade: disease spot area accounts for 16-25% of whole leaf area;

[0136] 7 grade: disease spot area accounts for 26-50% of whole leaf area;

[0137] 9 grade: disease spot area accounts for more than 50% of whole leaf area.

[0138] Data statistics and analysis:

[0139] Disease index is calculated according to formula (1)

[0140]

[0141] In the formula:

[0142] X - disease index;

[0143] N i - number of leaves at each grade;

[0144] i - relative grade value;

[0145] N - total number of leaves investigated.

[0146] Control effect is calculated according to formula (2)

[0147]

[0148] In the formula:

[0149] P - control effect, unit: %;

[0150] CK - disease index of blank control;

[0151] PT - disease index of chemical treatment.

[0152] According to the standard method of biological test NY / 1156.6-2006, the synergistic effect of mixed chemicals is evaluated according to the method of common toxicity coefficient (CTC) of Sun Yunpei, i.e. CTC≤80 for antagonistic effect, 80<CTC<120 for additive effect, and CTC≥120 for synergistic effect. The common toxicity coefficient (CTC) is calculated according to formula (3), formula (4) and formula (5).

[0153]

[0154] In the formula:

[0155] ATI - observed toxicity index of mixture;

[0156] S - EC of standard pesticide 50 in milligrams per liter (mg / L);

[0157] M - EC of mixture 50 in milligrams per liter (mg / L).

[0158] TTI = TI A x P A + TI B x P B

[0159] where:

[0160] TTI - theoretical toxicity index of mixture;

[0161] TI A - toxicity index of A pesticide;

[0162] P A - percentage content of A pesticide in mixture, in percentage (%);

[0163] TI B - toxicity index of B pesticide;

[0164] P B - percentage content of B pesticide in mixture, in percentage (%).

[0165]

[0166] where:

[0167] CTC - coefficient of toxicity;

[0168] ATI - observed toxicity index of mixture;

[0169] TTI - theoretical toxicity index of mixture.

[0170] The DPS data processing software was used to calculate the test results, and the toxicity regression equation, EC 50 of the test pesticide single agent and different ratio of mixture were calculated, and the coefficient of toxicity (CTC) of two kinds of pesticides with different ratio was calculated, and the best ratio of test pesticide was screened out. The original data of all replicates of each treatment were recorded.

[0171] The results of indoor test are shown in the following table:

[0172] Table 3 Indoor combined action test results of polyoxin B and bixafen on wheat powdery mildew

[0173] Test agent and ratio Regression equation (Y=) EC 50 (mg / L) Co-toxicity coefficient Polyoxin B y=4.6070+1.1420x 2.2087 - Bixafen y=4.0234+1.0634x 8.2866 - Polyoxin B: Bixafen = 1:48 y=4.2821+0.8620x 6.6469 118.040 Polyoxin B: Bixafen = 1:24 y=4.3391+0.8880x 5.5501 134.501 Polyoxin B: Bixafen = 1:12 y=4.2858+0.9870x 4.2913 159.368 Polyoxin B: Bixafen = 1:6 y=4.3153+1.3731x 3.1869 186.647 Polyoxin B: Bixafen = 1:2 y=4.5805+1.1719x 2.2923 188.548 Polyoxin B: Bixafen = 1:1 y=4.6899+1.0325x 1.9969 174.659 Polyoxin B: Bixafen = 2:1 y=4.8027+0.8251x 1.7341 168.586 Polyoxin B: Bixafen = 5:1 y=4.7224+0.9831x 1.9156 131.358 Polyoxin B: Bixafen = 10:1 y=4.7511+0.9393x 1.8409 128.551 Polyoxin B: Bixafen = 30:1 y=4.6974+1.0182x 1.9821 114.133 Polyoxin B: Bixafen = 50:1 y=4.6590+1.0851x 2.0617 108.693

[0174] Table 4 The results of the in vitro combined action test of polyoxin B and dimethomorph on wheat powdery mildew

[0175] Test agent and ratio Regression equation (Y=) EC 50 (mg / L)]]> Co-toxicity coefficient Polyoxin B y=4.6241+1.1057x 2.1877 - Flumetover y=4.4865+1.0562x 3.0632 - Polyoxin B: Flumetover = 1:40 y=4.6690+0.7712x 2.6865 112.920 Polyoxin B: Flumetover = 1:20 y=4.6743+0.8774x 2.3508 127.868 Polyoxin B: Flumetover = 1:10 y=4.7146+0.8651x 2.1376 138.270 Polyoxin B: Flumetover = 1:5 y=4.9114+1.2495x y = 4.7453 + 0.9467x 1.8582 154.540 Polyoxin B: flutianil = 1:2 y = 4.8532 + 0.8148x 1.5140 178.512 Polyoxin B: flutianil = 1:1 y = 4.8621 + 1.0521x 1.3524 188.736 Polyoxin B: flutianil = 2:1 y = 4.8305 + 0.8316x 1.5990 151.224 Polyoxin B: flutianil = 5:1 y = 4.7515 + 1.1063x 1.6773 136.954 Polyoxin B: flutianil = 10:1 y = 4.7707 + 0.8908x 1.8088 124.174 Polyoxin B: flutianil = 20:1 y = 4.7354 + 0.9554x 1.8920 117.224 Polyoxin B: flutianil = 40:1 y = 4.6832 + 0.9580x 2.1412 102.889

[0176] As shown by the results of the in vitro tests in Tables 3 and 4, the combination of polyoxin with either bifenoxycarb or dimethomorph has a significant synergistic effect on wheat powdery mildew within a reasonable range.

[0177] Field efficacy test

[0178] Example 3: Field efficacy test of different pesticides on wheat scab

[0179] Test basis: The test refers to NY / T 1464.15-2007 "Guidelines for Pesticide Field Efficacy Test Part 15: Fungicides for Controlling Wheat Scab".

[0180] Test target: Wheat scab (Fusarium graminearum).

[0181] Test crop: Wheat (Ningmai 13).

[0182] Test site: The test was carried out in a wheat field in Shaoxing City, Zhejiang Province. The soil fertility of the test site was high, and the wheat was sown on November 12, 2019, with a sowing amount of 18 kg per mu.

[0183] Test pesticides: The relevant test pesticides and application rates are shown in the table below.

[0184] Plot arrangement: The test pesticides, control pesticides and blank controls were arranged randomly.

[0185] Plot area and repetition: The plot area was 20 m 2 , and each treatment was repeated 4 times.

[0186] Application time and frequency: The first application was carried out at the early flowering stage of wheat, using a Gongnong-16 knapsack sprayer for conventional spraying, and uniform spraying was ensured.

[0187] Test investigation: The control effect was investigated at the milk stage of wheat, 5 points were sampled on the diagonal of each plot, 100 ears were investigated at each point, and the percentage of dry ear area to the whole ear area was used for grading, and the number of disease ears at each level and the total number of ears were recorded.

[0188] Grading method:

[0189] 0 level: Whole ear without disease;

[0190] 1 level: Dry ear area accounts for less than 1 / 4 of the whole ear area;

[0191] Grade 3: 1 / 4-1 / 2 of the whole ear area is dead;

[0192] Grade 5: 1 / 2-3 / 4 of the whole ear area is dead;

[0193] Grade 7: more than 3 / 4 of the whole ear area is dead.

[0194] Pharmacodynamic calculation method:

[0195]

[0196] Safety investigation: After administration, irregular observation was carried out, and no phytotoxicity such as yellowing, spotting and deformation of the wheat stems and leaves was observed for each treatment, and the wheat grew normally, indicating that the pesticide composition of the present application is safe to the wheat within the administration dose and administration frequency range of the present test.

[0197] The field efficacy test results are shown in Table 5:

[0198] Table 5 Field efficacy test results of different pesticide treatments on wheat scab

[0199] Treatment Active ingredient Amount (g / hm 2 )]]> Spike rate Disease index Control effect (%) 24% polyoxin B·bifenalize suspension concentrate (1:3) 12 13.80 2.03 89.32 42% polyoxin B·bifenalize wettable powder (1:5) 12 20.55 3.06 83.86 20% polyoxin B·flutianil suspension concentrate (1:1) 8 16.45 2.54 86.64 30% polyoxin B·flutianil water dispersible granules (1:2) 8 13.50 2.11 88.86 20% flutianil suspension concentrate 10 22.75 4.54 76.11 20% polyoxin B wettable powder 8 22.20 4.60 75.77 30% bifenalize suspension concentrate 30 24.50 5.47 71.18 Blank control - 62.95 18.99 /

[0200] As can be seen from the field efficacy test in Table 5, reasonable compounding of polyoxin B with bifenadime or fluazinam can effectively enhance the prevention and treatment effect on wheat scab. The wheat diseased ear rate of the compounding preparation treatment group of the present application is reduced, and the prevention and treatment effect is more than 83%.

[0201] Example 4: Field efficacy test of different treatment agents on wheat powdery mildew

[0202] Test basis: The test refers to GB / T 17980.22-2000 "Guidelines for Field Efficacy Test of Pesticides (I) Fungicides for Controlling Powdery Mildew of Cereals" to carry out the present test.

[0203] Test object: Wheat powdery mildew (Blumeria graminis f.sp.tritici).

[0204] Test crop: Wheat, variety is Shannong No. 22.

[0205] Test site: Xizhangcun wheat field in Tai'an City, Shandong Province, the previous crop before the test is corn, the soil fertility of the test site is medium, and the cultivation conditions (variety, soil type and fertilization) of the test plots are uniform and consistent, which conforms to the local scientific agricultural practice.

[0206] Test agent: The test agent, control agent and effective ingredient dosage are shown in the following table.

[0207] Test plot arrangement: the test agent, control agent and blank control plot treatment were arranged by random block, each plot area was 20 m 2 , each treatment was repeated 4 times.

[0208] Method of application: according to the test design, the application was carried out at the initial stage of powdery mildew, the application time was treated by conventional spraying with hand sprayer, the application was carried out twice, the interval of each application was 7 d.

[0209] Investigation method: five points were sampled on the diagonal line of each plot, 0.25 m 2 Wheat plant, after the wheat heading, the flag leaf and the first leaf under the flag leaf of each plant were investigated, and the classification was carried out according to the following classification method.

[0210] Classification standard:

[0211] 0 level: no disease;

[0212] 1 level: the diseased area accounted for less than 5% of the whole leaf area;

[0213] 3 level: the diseased area accounted for 6% to 15% of the whole leaf area;

[0214] 5 level: the diseased area accounted for 16% to 25% of the whole leaf area;

[0215] 7 level: the diseased area accounted for 26% to 50% of the whole leaf area;

[0216] 9 level: the diseased area accounted for more than 50% of the whole leaf area;

[0217] Investigation time: before the first spraying treatment, the disease index was investigated and recorded, the test was carried out 7 d after the first application, and the efficacy investigation was carried out 10 d after the last application.

[0218] Efficacy calculation method:

[0219] The efficacy was calculated according to the following formula:

[0220]

[0221] The field efficacy test results were shown in table 6:

[0222] Table 6 field efficacy test results of different treatment agents on wheat powdery mildew

[0223]

[0224] The test results in table 6 showed that the preparation examples of 35% polyoxin B · bifenpirim water dispersible granules (1:6) and 20% polyoxin B · fluazinam suspension concentrate (1:1) had good prevention and control effect on wheat powdery mildew, and were safe to wheat crops.

Claims

1. A pesticidal composition comprising polyoxin, characterized in that, The pesticide composition comprises active ingredient I and active ingredient II, the active ingredient I is polyoxin B, the active ingredient II is fluazinam, and the mass ratio of the active ingredient I to the active ingredient II is 1:30-10:

1.

2. The pesticidal composition according to claim 1, characterized in that, The mass ratio of the active ingredient I to the active ingredient II is 1:20-10:

1.

3. The pesticidal composition according to claim 1, characterized in that, The total weight of the pesticide composition is 100 wt%, and the total weight of the active ingredient I and the active ingredient II accounts for 1-90% of the total weight of the pesticide composition.

4. The pesticidal composition according to claim 3, characterized in that, The total weight of the pesticide composition is 100 wt%, and the total weight of the active ingredient I and the active ingredient II accounts for 2-80% of the total weight of the pesticide composition.

5. The pesticidal composition according to claim 1, characterized in that, The pesticide composition comprises, in addition to the active ingredients, an agriculturally acceptable auxiliary ingredient selected from one or more of wetting agents, dispersants, emulsifiers, thickening agents, disintegrants, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

6. The pesticidal composition according to claim 1, characterized in that, The pesticide composition is prepared into any one of agriculturally acceptable preparation forms, which is a solid preparation or a liquid preparation; the solid preparation is water dispersible granules or wettable powder, and the liquid preparation is a suspension.

7. The pesticide composition according to any one of claims 1-6 for use in the prevention and treatment of plant diseases.

8. Use according to claim 7, characterized in that, The plant is a cereal crop, and the disease is wheat scab or wheat powdery mildew.

9. Use according to claim 7, characterized in that, The pesticide composition is applied to the pathogenic fungi in need of prevention and treatment or the medium where the pathogenic fungi grow in an effective dose.

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