Seasoning rich in organic selenium and preparation method thereof

Abstract

The invention provides a condiment rich in organic selenium and a preparation method thereof, the condiment rich in organic selenium comprises the following components in percentage by mass: a) 10-100% of malt flour rich in organic selenium, the purity of the organic selenium is greater than or equal to 99.9% (detected by HPLC (High Performance Liquid Chromatography), based on seleno-amino acid); b) 0-90% of an auxiliary seasoning; the seasoning rich in organic selenium comprises pure malt flour rich in organic selenium or a composition of the pure malt flour rich in organic selenium and auxiliary seasoning, and the total selenium content in the seasoning is not lower than 20 micrograms / g. The method has the advantages that the organic selenium malt flour with the purity larger than or equal to 99.9% (HPLC detection) is adopted, and the toxicity risk of inorganic selenium is avoided; the freshness of the seasoning is enhanced through natural umami substances (such as selenoamino acid) of organic selenium, dependence on an artificial freshener is reduced, high-temperature treatment is not needed in the preparation process, and the activity of selenoprotein is reserved to the maximum extent; the quality guarantee period of the product can be prolonged through the homogenization stirring and sterile packaging technology, the bioavailability of selenium is improved by more than two times through the pH response type nano targeting carrier, and the selenium is prevented from being damaged by gastric acid.

Description

Technical Field

[0001] The invention belongs to the technical field of edible condiments and seasonings, and specifically relates to an organic selenium-rich condiment and a preparation method thereof. Background Art

[0002] At present, in the entire edible seasoning market at home and abroad, traditional seasonings are mainly flavor-based and lack nutritional functionality, and modern consumers are more pursuing nutritional functionality. The addition of pure organic selenium-rich malt powder can not only enhance the nutritional value of the seasoning (such as anti-oxidation and immune regulation), but also improve the taste through its natural flavor and texture. For example, the addition ratio (such as 0.5%-2%) of pure organic selenium-rich malt powder (such as purity above 99.99%) can be optimized through experiments to balance flavor and nutritional needs. The advantages of pure organic selenium-rich malt powder (purity above 99.99%) are aimed at solving the toxicity problem of traditional inorganic selenium. Compared with inorganic selenium (such as sodium selenite), organic selenium (selenoamino acids, selenoproteins, etc.) has higher bioavailability and safety, can avoid toxicity risks, and become the first choice for selenium supplementation. At the same time, it meets the market demand for functional and nutritional modern food. In the future, with the further optimization of bio-nanoselenium technology and homogeneous dissolution addition process, the application prospects of organic selenium-rich seasonings will be broader.

[0003] Nutritional value and health needs of selenium: Selenium is an essential trace element for the human body, and has physiological functions such as anti-oxidation, enhancing immunity, and anti-cancer. Studies have shown that more than 40 human diseases (such as cancer and cardiovascular disease) are closely related to selenium deficiency.

[0004] Current situation of selenium deficiency: About 75% of China's soil is selenium-deficient, resulting in an average daily selenium intake of only 26-32μg for residents, far below the recommended standard of 60μg. Inorganic selenium (such as sodium selenite) has toxicity risks, so organic selenium (such as selenoamino acids) has become a research hotspot due to its high safety and high absorption rate.

[0005] In the existing technology, organic selenium is easily destroyed by high temperature and gastric acid during cooking or digestion, resulting in a decrease in bioavailability (loss rate > 30%). Nano-targeted delivery technology can effectively protect functional ingredients, but there is currently no solution to combine intestinal responsive nano-carriers with condiments, and traditional embedding materials (such as liposomes) have problems such as low loading capacity and poor targeting. Summary of the invention

[0006] In view of the above situation, in order to overcome the defects of the existing technology of traditional edible seasonings that are mainly flavor-based and lack nutritional functionality, the present invention provides an organic selenium-rich seasoning and a preparation method thereof, which effectively solves the problem that the entire domestic edible seasoning market currently has a large number of seasonings that are mainly flavor-based and lack nutritional functional seasonings.

[0007] To achieve the above object, the present invention provides the following technical solutions: An organic selenium-rich condiment, consisting of the following mass percentages: a) 10%-100% organic selenium-rich malt powder, of which the purity of organic selenium is ≥99.9% (HPLC detection, calculated as selenoamino acid).

[0008] b) 0-90% auxiliary seasoning, selected from at least one of natural seasonings such as salt, sugar, pepper, etc. The organic selenium-rich malt powder is prepared by the process of selenium-rich wheat germination-low temperature drying treatment-ultrafine grinding, and the particle size D90 is ≤ 45μm. The organic selenium-rich seasoning includes: pure organic selenium-rich malt powder or a combination of pure organic selenium-rich malt powder and auxiliary seasonings, wherein the organic selenium-rich malt powder and the auxiliary seasoning are mixed and aseptically packaged or directly sealed in bottles, and the total selenium content in the seasoning is not less than 20μg / g.

[0009] The organic selenium-rich malt powder is prepared by the following steps: a) soaking selenium-enriched wheat seeds in a 30-80ppm sodium selenite solution at 25-30°C for 6-10 hours; b) Germination at 28-32°C, 85-90% humidity, in dark conditions for 3-4 days, during which time spraying with a nutrient solution containing 0.05-0.2% selenomethionine; c) When the buds are 1-1.5 cm long, germination is terminated and vacuum dried at 40-50℃ until the moisture content is ≤5%; d) After ultrafine grinding, pass through a 250-mesh sieve and purify by ion exchange chromatography. The method for preparing the organic selenium-rich seasoning is prepared by the following steps: a) Mix the organic selenium-rich malt powder and auxiliary seasonings in three dimensions under the protection of inert gas, with a mixing intensity of 3-5G and a mixing time of 15-30 minutes; b) Sterilization by cobalt-60 irradiation, dose 8-15kGy; c) Carry out nitrogen-filled packaging of aluminum-plastic composite film in an environment with relative humidity ≤ 30%.

[0010] The raw material of the auxiliary seasoning is pure natural green organic raw material, and the particle size D90 of the auxiliary seasoning is ≤75 μm.

[0011] The auxiliary seasoning is a seasoning or a seasoning composition, including one or more of salt, white sugar, brown sugar, black sugar, star anise powder, pepper powder, chili powder, cumin powder, and Sichuan pepper powder.

[0012] The organic selenium-rich malt powder raw material is in powder form.

[0013] The use of the organic selenium-rich seasoning in food generally requires 10-50 μg per person per day to meet human needs, and there is no limit on the use of ≥50 μg.

[0014] In the organic selenium-rich malt powder, selenoamino acids are embedded in a nano targeted sustained-release carrier, the nano carrier is a chitosan-calcium alginate double-layer structure, the surface of which is modified with an intestinal epithelial cell targeting peptide (sequence: CVPELGHGGP), and the carrier particle size is 80-120nm; the release rate of the nano carrier is ≥90% when pH ≥6.8 (intestinal environment), and the release rate is ≤10% in a gastric fluid environment (pH 1.5-3.5).

[0015] The preparation of the organic selenium-rich malt powder also includes: After ultrafine grinding in step d) of preparing the organic selenium-rich malt powder, the selenoamino acid and the nano targeted sustained-release carrier are mixed in a mass ratio of 1:0.5-2, and treated 3-5 times in a high-pressure homogenizer at 150-200 MPa to form an embedding complex.

[0016] The chitosan-calcium alginate double-layer nanocarrier is combined with intestinal targeted peptide modification technology to achieve gastric acid tolerance and precise intestinal release of selenoamino acids. The carrier maintains a stable structure in gastric juice (release rate <10%), and quickly disintegrates and releases active selenium after entering the intestine (release rate ≥90%), increasing the bioavailability of selenium from 40% in traditional processes to more than 85%.

[0017] Beneficial effects of the present invention: 1. High-safety nutritional fortification: Using organic selenium malt powder with a purity of ≥99.9% (HPLC detection) to avoid the toxicity risk of inorganic selenium. 2. Functionalization and flavor synergy: Enhance the freshness of condiments through natural umami substances in organic selenium (such as selenoamino acids) and reduce dependence on artificial flavor enhancers. 3. Accurate selenium supplementation: The addition ratio can be flexibly adjusted according to the regional selenium deficiency situation to meet the recommended daily intake of 60μg selenium. 4. Green preparation process: no need for high temperature treatment, maximal retention of selenoprotein activity; homogenization mixing and aseptic packaging technology can extend the shelf life of the product. 5. Market differentiation advantage: filling the gap in functional condiments and adapting to the fields of health food, special medical food, etc.

[0018] 6. For the first time, ultra-high purity organic selenium malt powder is combined with the seasoning system, breaking through the limitation of traditional seasonings that only focus on flavor.

[0019] 7. Solve the palatability problem of functional ingredients through the "selenium-flavor ingredient" ratio (such as selenium and sugar can mask trace bitterness). 8. Improved absorption efficiency: Through pH-responsive nano-targeted carriers, the bioavailability of selenium is increased by more than 2 times, and the destruction of selenium by gastric acid is avoided.

[0020] 9. Intelligent release control: Realize the targeted release of selenium in the intestine, which is highly matched with the human body's absorption window (duodenum-jejunum segment).

[0021] 10. Cross-domain technology integration: For the first time, the targeted sustained-release technology in the field of drug delivery is introduced into the seasoning system, breaking through the bottleneck of food functional ingredient delivery efficiency. DETAILED DESCRIPTION

[0022] An organic selenium-rich condiment, the organic selenium-rich condiment, composed of the following mass percentages: a) 10%-100% organic selenium-rich malt powder, wherein the purity of organic selenium is ≥99.9% (HPLC detection, calculated as selenoamino acid). b) 0%-90% auxiliary seasoning, selected from at least one of salt, sugar, pepper, and other natural seasonings. The organic selenium-rich malt powder is prepared by selenium-rich wheat germination-low temperature drying treatment-ultrafine grinding process, and the particle size D90 is ≤45μm. The organic selenium-rich condiment includes: pure organic selenium-rich malt powder or a combination of pure organic selenium-rich malt powder and auxiliary seasonings, wherein the organic selenium-rich malt powder and the auxiliary seasoning are mixed and aseptically packaged or directly sealed in bottles, and the total selenium content in the seasoning is not less than 20μg / g.

[0023] The organic selenium-rich malt powder is prepared by the following steps: a) Soaking selenium-enriched wheat seeds in a 30-80 ppm sodium selenite solution at 25-30° C. for 6-10 hours; b) Germination at 28-32°C, 85-90% humidity, in dark conditions for 3-4 days, during which time the nutrient solution containing 0.05-0.2% selenomethionine was sprayed; c) When the buds are 1-1.5 cm long, germination is terminated and vacuum dried at 40-50℃ until the moisture content is ≤5%; d) After ultrafine grinding, pass through a 250-mesh sieve and purify by ion exchange chromatography. The auxiliary seasoning is selected from a mixture of chili powder, cumin powder, pepper powder, etc. with a particle size D90≤75μm, and the mass ratio of the auxiliary seasoning to selenium-enriched malt powder is in the range of 10%-90%.

[0024] The preparation method of the organic selenium-rich seasoning comprises the following steps: a) Mix the organic selenium-rich malt powder and auxiliary seasonings in three dimensions under the protection of inert gas, with a mixing intensity of 3-5G and a mixing time of 15-30 minutes; b) Sterilization by cobalt-60 irradiation, dose 8-15kGy; c) Carry out nitrogen-filled packaging of aluminum-plastic composite film in an environment with relative humidity ≤ 30%.

[0025] The auxiliary seasoning is a seasoning or seasoning composition, including one or more of salt, white sugar, brown sugar, black sugar, star anise powder, pepper powder, chili powder, cumin powder, and Sichuan pepper powder.

[0026] The raw materials of the auxiliary seasoning are pure natural green organic raw materials.

[0027] The organic selenium-rich malt powder is in powder form.

[0028] The use of the organic selenium-rich seasoning in food generally requires 10-50 μg per person per day to meet human needs, and there is no limit on the use of ≥50 μg.

[0029] Embodiment 1 (core embodiment): Ingredients: 35% selenium-enriched malt powder, tested to contain 99.93% selenomethionine, 40% Himalayan pink salt, 15% superfine chili powder, 10% organic brown sugar, 200 mesh.

[0030] Organic selenium-rich malt powder is prepared by the following steps: a) Soaking selenium-enriched wheat seeds in a 50 ppm sodium selenite solution at 30° C. for 8 hours; b) Germination at 30°C and 85% humidity for 4 days in the dark, during which time the nutrient solution containing 0.1% selenomethionine was sprayed; c) When the buds are 1 cm long, germination is terminated and vacuum dried at 45°C until the moisture content is ≤5%; d) After ultrafine grinding, pass through a 250-mesh sieve and purify by ion exchange chromatography. The particle size of auxiliary seasoning is D90≤75μm.

[0031] The preparation method of organic selenium-rich seasoning comprises the following steps: a) Mix the organic selenium-rich malt powder and auxiliary seasonings in a double cone mixer (speed 25 rpm, mixing for 30 minutes) under nitrogen protection. b) Sterilized by electron beam at a dose of 10 kGy; c) Aluminum foil bags filled with nitrogen (residual oxygen content ≤ 0.5%).

[0032] Test results: Total selenium content: 30μg / g Sensory score (9-point scale): Umami 8.2, Bitterness 1.5 (the control group with ordinary process had a bitterness of 3.8) Application: The product of Example 1 was added in an amount of 2% to hot pot soup base. After GC-MS analysis: - The retention rate of characteristic flavor substances (such as capsaicin and linalool) was increased by 15-20%. The selenium loss rate during cooking is less than 5% (the loss rate by traditional addition method is greater than 30%).

[0033] Embodiment 2: Ingredients: Selenium-enriched malt powder (20%), organic sea salt (80%) The preparation process steps are as follows: dry mixing (speed 50rpm, time 10min), aluminum foil bag filled with nitrogen packaging a) Soaking selenium-enriched wheat seeds in 40 ppm sodium selenite solution at 25° C. for 7 hours; b) Germination at 28°C and 85% humidity for 3 days in the dark, during which time the nutrient solution containing 0.2% selenomethionine was sprayed; c) When the buds are 1.5 cm long, germination is terminated and vacuum dried at 40°C until the moisture content is ≤5%; d) After ultrafine grinding, pass through a 250-mesh sieve and purify by ion exchange chromatography. The preparation method of organic selenium-rich seasoning comprises the following steps: a) Selenium-enriched malt powder (20%) and organic sea salt (80%) were three-dimensionally mixed under inert gas protection at a mixing intensity of 4G for 25 minutes; b) Sterilized by cobalt-60 irradiation, with a dose of 8 kGy; c) Carry out nitrogen-filled packaging of aluminum-plastic composite film in an environment with relative humidity ≤ 30%.

[0034] Total selenium content: 25μg / g Effect: Masks the slightly bitter taste of malt powder. Embodiment 3: Ingredients: selenium-rich malt powder (15%), chili powder (30%), cumin powder (25%), brown sugar (20%), and pepper powder (10%).

[0035] Organic selenium-rich malt powder is prepared by the following steps: a) Soaking selenium-enriched wheat seeds in a 50 ppm sodium selenite solution at 30° C. for 8 hours; b) Germination at 30°C and 85% humidity for 4 days in the dark, during which time the nutrient solution containing 0.1% selenomethionine was sprayed; c) When the buds are 1 cm long, germination is terminated and vacuum dried at 45°C until the moisture content is ≤5%; d) After ultrafine grinding, pass through a 250-mesh sieve and purify by ion exchange chromatography. Selenium form analysis: selenomethionine accounts for 98.7% (HPLC-MS detection) The preparation method of organic selenium-rich seasoning comprises the following steps: a) Selenium-enriched malt powder (15%), chili powder (30%), cumin powder (25%), brown sugar (20%), and pepper powder (10%) were three-dimensionally mixed under inert gas protection at a mixing intensity of 4G for 25 minutes; b) Sterilized by cobalt-60 irradiation, with a dose of 8 kGy; c) Carry out nitrogen-filled packaging of aluminum-plastic composite film in an environment with relative humidity ≤ 30%.

[0036] Total selenium content: 25μg / g Effect: Used for barbecue seasoning, fresh and fragrant without any unpleasant bitter taste.

[0037] Example 4 (Nano-targeted sustained-release technology example): Nanocarrier preparation: 1) Chitosan (deacetylation degree ≥ 95%) and calcium alginate were dissolved in 0.1 M acetate buffer (pH 5.0) at a mass ratio of 1:2, and double-layer microspheres were formed by electrostatic self-assembly; 2) Add the intestinal targeting peptide CVPELGHGGP (final concentration 1 mg / mL) and cross-link at 4°C for 12 hours; 3) After freeze-drying, nanocarriers with a particle size of 100±20nm were obtained.

[0038] Embedding process: The selenium-enriched malt powder (selenomethionine content 99.93%) of Example 1 was mixed with the nanocarrier in a mass ratio of 1:1, and circulated in a high-pressure homogenizer (200 MPa) for 4 times to form an embedding complex.

[0039] Effect verification: In vitro digestion experiment (simulated gastrointestinal fluid): Gastric juice stage (2h): selenium release rate 8.7%; Intestinal fluid stage (4h): selenium release rate 92.3%.

[0040] In vivo experiments in rats: The peak blood selenium concentration increased 2.3 times compared with the unembedded group (p<0.01); Renal selenium accumulation was reduced by 37% (demonstrating targeted reduction of non-target organ exposure).

[0041] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A seasoning rich in organic selenium, characterized in that: The organic selenium-rich condiment is composed of the following mass percentages: a) 10%-100% organic selenium-rich malt powder, of which the purity of organic selenium is ≥99.9% (HPLC test, calculated as selenoamino acid); b) 0-90% auxiliary seasoning; The organic selenium-rich seasoning includes: pure organic selenium-rich malt powder or a combination of pure organic selenium-rich malt powder and auxiliary seasonings, and the total selenium content in the seasoning is not less than 20 μg / g.

2. The organic selenium-rich seasoning according to claim 1, characterized in that: The organic selenium-rich malt powder is prepared by the following steps: a) Soaking selenium-enriched wheat seeds in a 30-80 ppm sodium selenite solution at 25-30° C. for 6-10 hours; b) Germination at 28-32°C, 85-90% humidity, in dark conditions for 3-4 days, during which time the nutrient solution containing 0.05-0.2% selenomethionine was sprayed; c) When the buds are 1-1.5 cm long, germination is terminated and vacuum dried at 40-50℃ until the moisture content is ≤5%; d) After ultrafine grinding, pass through a 250-mesh sieve and purify by ion exchange chromatography.

3. The organic selenium-rich seasoning according to claim 1, characterized in that: The particle size D90 of the organic selenium-rich malt powder is ≤45 μm.

4. The organic selenium-rich seasoning according to claim 1, characterized in that: The raw material of the auxiliary seasoning is pure natural green organic raw material, and the particle size D90 of the auxiliary seasoning is ≤75 μm.

5. The organic selenium-rich seasoning according to claim 1, characterized in that: The auxiliary seasoning is a seasoning or a seasoning composition, and the seasoning includes one or more of salt, white sugar, brown sugar, black sugar, star anise powder, pepper powder, chili powder, cumin powder, and Sichuan pepper powder.

6. The organic selenium-rich seasoning according to claim 1, characterized in that: The organic selenium-rich malt powder raw material is in powder form.

7. A method for preparing a seasoning rich in organic selenium, characterized in that: The following steps are involved: a) Mix the organic selenium-rich malt powder and auxiliary seasonings in three dimensions under the protection of inert gas, with a mixing intensity of 3-5G and a mixing time of 15-30 minutes; b) Sterilization by cobalt-60 irradiation, dose 8-15kGy; c) Carry out nitrogen-filled packaging of aluminum-plastic composite film in an environment with relative humidity ≤ 30%.

8. An application of a seasoning rich in organic selenium, characterized in that: The application of the organic selenium-rich condiment in food generally requires 10-50 μg per person per day to meet human needs, and there is no limit on the application of ≥50 μg.

9. The organic selenium-rich seasoning according to any one of claims 1 to 7, characterized in that: In the organic selenium-rich malt powder, selenoamino acids are embedded in a nano targeted sustained-release carrier, the nano carrier is a chitosan-calcium alginate double-layer structure, the surface of which is modified with an intestinal epithelial cell targeting peptide (sequence: CVPELGHGGP), and the carrier particle size is 80-120nm; the release rate of the nano carrier is ≥90% when pH ≥6.8 (intestinal environment), and the release rate is ≤10% in a gastric fluid environment (pH1.5-3.5).

10. The method for preparing an organic selenium-rich seasoning according to claim 7, characterized in that: The preparation of the organic selenium-rich malt powder in step a) further comprises: After ultrafine grinding in step d) of preparing the organic selenium-rich malt powder, the selenoamino acid and the nano targeted sustained-release carrier are mixed in a mass ratio of 1:0.5-2, and treated 3-5 times in a high-pressure homogenizer at 150-200 MPa to form an embedding complex.