Composition for relieving depression as well as preparation method and application thereof

Abstract

The invention provides a composition for relieving depression. The composition comprises 10-17 parts of anise, 10-15 parts of fennel, 9-12 parts of balm, 18-25 parts of seville orange flower, 8-15 parts of clove, 12-20 parts of black sesame, 12-16 parts of fingered citron, 5-17 parts of hawthorn, 15-20 parts of turmeric, 5-10 parts of pumpkin, 5-10 parts of red bean, 15-20 parts of dropped citrus fruit, 5-17 parts of walnut and 4-7 parts of medlar. The preparation method comprises the steps of crushing, water distillation, physical field treatment, decoction, vacuum concentration, drying, liposome embedding, spray drying into powder and the like. A multi-aspect anti-depression formula is constructed, and compared with health-care products / medicines with single or few components in the prior art, the anti-depression composition can more effectively recover liver functions and relieve depressive symptoms, and the problem that the effect is not remarkable due to short action time, drug resistance and large side effects of a single component in an existing product is solved.

Description

Technical Field

[0001] The invention belongs to the technical field of biological metabolism and food science, and in particular relates to a composition for relieving depression, and a preparation method and application thereof. Background Art

[0002] Depression is the second largest disease causing years of healthy life lost. Currently, taking antidepressants is the main strategy for controlling depressive episodes. However, clinical evidence shows that the efficacy of antidepressants is not ideal. Only 40% of patients achieve clinical efficacy after taking antidepressants for the first time, and the effect of antidepressants will not be apparent until two weeks after taking them. In addition, most antidepressants take effect gradually after about two weeks of medication. In addition, patients are prone to adverse reactions such as vomiting and suicidal tendencies for a period of time after taking the medication.

[0003] Active ingredients such as food-borne plant polyphenols and essential oils have unique advantages in preventing and assisting the treatment of depression. Animal and clinical studies have found that active ingredients such as food-borne plant polyphenols and essential oils have significant effects in preventing and assisting the treatment of depression, with few toxic side effects. It is worth noting that compared with chemical drugs, food-borne plant essential oils have unique advantages, including multiple targets, high pharmacological activity, easy passage through the blood-brain barrier, and multiple routes of administration, and have attracted widespread attention from scholars at home and abroad.

[0004] Although polyphenols have significant potential in alleviating depression, many polyphenols are generally large in size and have complex structures. They cannot easily pass through the intestinal wall into the blood, and they have low solubility in water. Low water solubility and complex structure limit their absorption, resulting in low bioavailability. Summary of the invention

[0005] The technical problem to be solved by the present invention is to overcome the deficiencies in the prior art and provide a composition for relieving depression and a preparation method and application thereof.

[0006] In order to solve the above technical problems, the present invention provides a composition for relieving depression, comprising: 10-17 parts of star anise, 10-15 parts of fennel, 9-12 parts of lemon balm, 18-25 parts of daikon jasmine, 8-15 parts of cloves, 12-20 parts of black sesame, 12-16 parts of bergamot, 5-17 parts of hawthorn, 15-20 parts of turmeric, 5-10 parts of pumpkin, 5-10 parts of red bean, 15-20 parts of citrus fruit drops, 5-17 parts of walnuts, and 4-7 parts of wolfberry.

[0007] The above composition further comprises: 13 parts of star anise, 12 parts of fennel, 10 parts of lemon balm, 20 parts of daidai flower, 11 parts of cloves, 12 parts of black sesame, 15 parts of bergamot, 7 parts of hawthorn, 15-20 parts of turmeric, 6 parts of pumpkin, 6 parts of red bean, 16 parts of citrus fruit drops, 9 parts of walnuts, and 4 parts of wolfberry.

[0008] The above-mentioned composition further includes essential oils and polyphenol extracts, which are obtained by extracting the following raw materials: 10 to 17 parts of star anise, 10 to 15 parts of fennel, 9 to 12 parts of lemon balm, 18 to 25 parts of daikon jasmine, 8 to 15 parts of cloves, 12 to 20 parts of black sesame, 12 to 16 parts of bergamot, 5 to 17 parts of hawthorn, 15 to 20 parts of turmeric, 5 to 10 parts of pumpkin, 5 to 10 parts of red beans, 15 to 20 parts of citrus fruits, 5 to 17 parts of walnuts, and 4 to 7 parts of wolfberries.

[0009] Based on a general technical concept, the present invention also provides a method for preparing the composition, the preparation method comprising:

[0010] S1, star anise, fennel, lemon balm, daidai flower, clove, black sesame, bergamot, hawthorn, turmeric, pumpkin, red bean, citrus fruit, walnut and wolfberry are crushed to obtain material 1;

[0011] S2, distilling the material 1 on water to obtain essential oil and material 2;

[0012] S3, mixing the material 2 with ethanol, subjecting it to physical field treatment, and then decocting it, and concentrating the filtrate obtained by decompression and drying it to obtain a polyphenol extract;

[0013] S4, mixing the essential oil and the polyphenol extract, embedding them in liposomes, and spray drying them into powder.

[0014] The above preparation method, further, in S2, the mass ratio of the material 1 to water is 1:1-6, and the time of the water distillation is 2-5h.

[0015] In the above preparation method, further, the physical field treatment in S3 includes one or more of ultrasonic treatment, microwave treatment, and ultra-high pressure treatment;

[0016] The power of the ultrasonic treatment is 20 to 100 kHz, the temperature is 30 to 90° C., and the treatment time is 10 s to 2 h.

[0017] The microwave treatment has a power of 50 to 500 W, a temperature of 30 to 90° C., and a treatment time of 10 s to 2 h.

[0018] The intensity of the ultra-high pressure treatment is 50-800 MPa, the temperature is 30-90° C., and the treatment time is 10 s to 2 h.

[0019] In the above preparation method, further, the material 2 in S3 is mixed with ethanol in a mass ratio of 1:3 to 10; the concentration of ethanol is 30% to 90%.

[0020] In the above preparation method, further, the decoction time in S3 is 2 to 4 hours, the extraction times are 1 to 3 times; the temperature for reduced pressure concentration is 30 to 60° C.; and the drying temperature is -40° C. to 80° C.

[0021] The above preparation method, further, in S4, the essential oil and polyphenol extract are mixed in a vortex mixer for 10s to 5min, and the essential oil and polyphenol mixture is embedded by inclusion technology, nanotechnology and / or microencapsulation technology to prepare particles with a particle size of about 150 to 500nm.

[0022] Based on a general technical concept, the present invention also provides an application of the composition in preparing food, medicine or health care product for regulating depression of the liver-brain axis.

[0023] Compared with the prior art, the advantages of the present invention are:

[0024] (1) The present invention provides a composition for relieving depression. By combining a variety of food-borne antidepressant substances (such as lemon balm, turmeric, daisy flower, bergamot, etc.), a multi-faceted antidepressant formula is constructed. Compared with the health products / drugs with a single or a few ingredients in the prior art, the present invention can more effectively restore liver function and relieve depression symptoms, avoiding the problem of insignificant effect caused by the short action time of a single ingredient in the existing products. The composition of the present invention is all food-borne substances and has no significant toxic effect on the body.

[0025] (2) The present invention provides a composition for relieving depression, and the antidepressant active ingredients (essential oils and polyphenols) of the composition are separated by a green extraction method. Compared with the composition, the essential oils and polyphenols of any one of the compositions have better antidepressant effects; since the water solubility of polyphenols is low, resulting in low bioavailability and poor biological effects, the essential oils are fat-soluble, which helps the polyphenols dissolve, helps the polyphenols enter the digestive tract and be absorbed, and improves their bioavailability; the essential oils and polyphenols of the composition are used together to further enhance the antidepressant effect, and the mechanism is that the essential oils and polyphenols of the composition jointly regulate liver metabolites, and the liver metabolites are transported through the blood, cross the blood-brain barrier and enter the brain, affecting the brain's synthesis of neurotransmitters, thereby relieving depressive symptoms.

[0026] (3) The present invention provides a method for preparing a composition for relieving depression, which adopts inclusion technology, nanotechnology and microencapsulation technology to significantly improve the absorption rate and stability of essential oils and polyphenols, solve the problems of instability of existing essential oils and low bioavailability of essential oils and polyphenols, ensure that the product effect is more lasting and stable, significantly improve the neuroprotective effect, and overcome the shortcomings of traditional products.

[0027] (4) The present invention provides a composition for relieving depression for use in the preparation of food, medicine or health care products for depression by regulating the liver-brain axis. The liver-brain axis refers to the interaction and communication network between the liver and the brain, which affects the nervous system, emotions and behavior through various mechanisms. The liver is an important metabolic organ and is also involved in the synthesis of neurotransmitter precursors, such as tryptophan, tyrosine, etc., which are the basis for the synthesis of important neurotransmitters (such as serotonin and dopamine). Decreased liver function may lead to a decrease in the synthesis of these precursors, thereby affecting the level of neurotransmitters, and thus leading to depressive symptoms. In patients with depression, inflammatory response is considered to be an important pathophysiological mechanism. The liver participates in inflammatory response by producing cytokines, chemokines, etc. These inflammatory factors can affect brain function through blood circulation, thereby affecting emotions and behavior. Liver dysfunction may lead to increased permeability of the blood-brain barrier, making it easy for certain harmful substances or inflammatory factors to enter the brain, affecting neural function and causing emotional disorders. The composition of the present invention may help relieve depressive symptoms and improve the quality of life of patients by improving liver health (such as diet improvement, drug intervention, exercise, etc.). This provides a theoretical basis for regulating the liver-brain axis to become a new strategy for the treatment of depression. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to make the purpose, technical solution and advantages of the embodiments of the present invention more clear, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0029] Figure 1 The figures are the results of animal behavior experiments in Experiment 1 of the present invention, wherein A in the figure is the result of the forced swimming experiment; B in the figure is the result of the tail suspension experiment; and C in the figure is the result of the sucrose preference rate experiment.

[0030] Figure 2 These are the results of serum TG and LDL-C levels in mice in Experiment 2 of the present invention.

[0031] Figure 3 This is the heat map of liver lipid metabolism of the composition and essential oil + polyphenol group in Experiment 3 of the present invention.

[0032] Figure 4 These are the results that the composition and essential oil + polyphenol group in Experiment 3 of the present invention regulated the liver-brain axis.

[0033] Figure 5 This is a schematic diagram showing that the composition and essential oil + polyphenol group in Experiment 3 of the present invention regulated the liver-brain axis through the lipid metabolism pathway. DETAILED DESCRIPTION

[0034] The present invention is further described below in conjunction with specific preferred embodiments, but the protection scope of the present invention is not limited thereby.

[0035] The materials, reagents and instruments used in the following examples can all be purchased from commercial channels. The experimental methods in the following examples are all conventional methods in the art unless otherwise specified. The units "parts" in the following examples are all parts by mass.

[0036] Example 1

[0037] A composition for relieving depression comprises: 13 parts of star anise, 12 parts of fennel, 10 parts of lemon balm, 20 parts of daisy flower, 11 parts of cloves, 12 parts of black sesame, 15 parts of bergamot, 7 parts of hawthorn, 17 parts of turmeric, 6 parts of pumpkin, 6 parts of red bean, 16 parts of citrus fruit drops, 9 parts of walnuts and 4 parts of wolfberry.

[0038] The preparation method comprises the following steps: preparing the above composition, drying it to a moisture content of less than 3.0%, and crushing it into powder.

[0039] Example 2

[0040] A composition for relieving depression comprises: 13 parts of star anise, 12 parts of fennel, 10 parts of lemon balm, 20 parts of daisy flower, 11 parts of cloves, 12 parts of black sesame, 15 parts of bergamot, 7 parts of hawthorn, 17 parts of turmeric, 6 parts of pumpkin, 6 parts of red bean, 16 parts of citrus fruit drops, 9 parts of walnuts and 4 parts of wolfberry.

[0041] The preparation method comprises the following steps:

[0042] (1) Dry the star anise, fennel, lemon balm, daidai flower, clove, black sesame, bergamot, hawthorn, turmeric, pumpkin, red bean, citrus fruit, walnut, and wolfberry in the above composition to a moisture content of less than 3.0%, grind them, and mix them according to proportion to obtain material 1;

[0043] (2) After distilling material 1 on water, essential oil is obtained, and the remaining material is collected and named as material 2;

[0044] (3) Material 2 was mixed with 9 times the mass of 65% (mass percentage) ethanol, and subjected to ultrasonic treatment at a power of 400 MPa for 20 min. Polyphenols were then extracted at 80° C. The extraction was performed twice, each time for 3 h. The filtrate was concentrated under reduced pressure at 40° C., and the polyphenol extract was obtained after freeze-drying.

[0045] (4) The essential oil and polyphenols were vortexed for 2 minutes to mix, and the composition was embedded in liposomes and spray-dried into powder.

[0046] Comparative Example 1

[0047] A composition for relieving depression comprises: 22 parts of daidaihua, 13 parts of black sesame, 18 parts of turmeric, 5 parts of red beans and 4 parts of wolfberries, which are prepared into powder.

[0048] Comparative Example 2

[0049] A composition for relieving depression comprises: 13 parts of star anise, 12 parts of fennel, 10 parts of lemon balm, 20 parts of daisy flower, 11 parts of cloves, 12 parts of black sesame, 15 parts of bergamot, 7 parts of hawthorn, 11 parts of turmeric, 6 parts of pumpkin, 6 parts of red bean, 16 parts of citrus fruit drops, 9 parts of walnuts and 4 parts of wolfberry.

[0050] The preparation method comprises the following steps: adding water of 5 times the mass of the mixed material, using water-distilled essential oil, using liposome embedding, and spray drying into powder.

[0051] Comparative Example 3

[0052] A composition for relieving depression comprises: 13 parts of star anise, 12 parts of fennel, 10 parts of lemon balm, 20 parts of daisy flower, 11 parts of cloves, 12 parts of black sesame, 15 parts of bergamot, 7 parts of hawthorn, 11 parts of turmeric, 6 parts of pumpkin, 6 parts of red bean, 16 parts of citrus fruit drops, 9 parts of walnuts and 4 parts of wolfberry.

[0053] The preparation method comprises the following steps: mixing the mixed material with 9 times the mass of 65% ethanol, subjecting the mixed material to ultrasonic treatment at a power of 400MPa for 20 minutes, extracting polyphenols at 80°C, extracting twice, each time for 3 hours, concentrating the filtrate under reduced pressure at 40°C, freeze-drying to obtain a polyphenol extract, embedding with liposomes, and spray drying to obtain powder.

[0054] Experiment 1: Investigate the effect of alleviating depressive symptoms in animals.

[0055] Experimental methods:

[0056] 1. Grouping: Male C57BL / 6 mice (3 weeks old, 8 mice per cage) were raised under standard conditions (humidity: 40%-60%, temperature: 20-25°C, 12 / 12h light-dark cycle) for one week, with free access to food and water. According to body weight, the mice were divided into: blank control group (normal saline, ig), animal chronic unpredictable stress (CUMS) model group (normal saline, ig), fluoxetine group (10 mg / kg, ig), treatment group of Example 2 (100 mg / kg, ig), etc. Gavage once a day for four consecutive weeks.

[0057] 2. Establishment of depression model: During the experiment, blank control mice were placed alone in an undisturbed environment, and no other operations were performed except routine care (such as cage cleaning). Other mice were placed in separate cages (one mouse per cage) and subjected to 7 weeks of chronic unpredictable stress stimulation. Daily stressors included: restraint for 4 hours; wet pad for 24 hours; fasting for 24 hours; water deprivation for 24 hours; hanging tail for 2 minutes; forced swimming for 5 minutes; reversal of light / dark cycle; cage tilt (45°) for 24 hours; cold stimulation for 1 hour (4°C); plantar stimulation for 1 minute; strobe for 12 hours; crowded housing for 24 hours. 12 stimuli were randomly given, two per day. All stimuli were performed at different times in a random order, and different stimuli were provided on two consecutive days.

[0058] 3. Depressive behavior assessment:

[0059] 3.1. Sucrose preference test: The sucrose preference experiment consists of two parts: adaptation training and testing. During the training process, two bottles of 1% (w / v) sucrose solution were placed in each cage within the first 24 hours, and one of the bottles was replaced with pure water within the next 24 hours. After food and water were removed for 18 hours, a bottle of 1% (w / w) sucrose solution and a bottle of water solution were placed in each cage within the next 25 hours. Each bottle was weighed before and after the test, and the total liquid consumption, sucrose consumption, and pure water consumption were recorded. The formula for the sucrose preference index is as follows: Sucrose preference (%) = sucrose water consumption / (sucrose water consumption + pure water consumption).

[0060] 3.2 Forced swimming test: Place mice individually in a cylindrical acrylic container (45 cm high, 10 cm in diameter) with a water depth of 30 cm and a temperature of 25 ± 1.0 ° C. Place each mouse in the container and record the swimming time with a total duration of 6 min. After a 1-min adaptation period, the duration of immobility is measured in the last 5 min. The water is changed immediately after each test to prevent odor interference, and the mice tested are separated by partitions to avoid mutual interference.

[0061] 3.3 Tail suspension test: The mice were suspended 60 cm from the ground for 6 min, with a fixing tape placed 1 cm from the tip of the tail, while being recorded with real-time video. The duration of immobility in the last 5 min was calculated and analyzed. Mice tested simultaneously were separated by partitions to reduce mutual interference. After each test, the area around the equipment was cleaned with 75% ethanol and the equipment was wiped to eliminate any influence of feces and avoid odor interference.

[0062] 4. Experimental results:

[0063] Figure 1The figures are the results of the animal behavior experiment of Experiment 1 of the present invention. A in the figure is the result of the forced swimming experiment. In the forced swimming experiment, the mice in Example 2 group showed the best antidepressant effect, which was significantly higher than that in Comparative Example 1 and Comparative Example 3 groups, which indicates that the Example group effectively alleviated the depressive state of the mice.

[0064] B in the figure is the result of the tail suspension experiment. In the tail suspension experiment, the struggling time of the mice in Example 2 group was much longer than that of all the control groups, reflecting its strong antidepressant effect.

[0065] C in the figure is the experimental result of sucrose preference rate. In terms of sucrose preference rate, the preference rate of Example 2 group was 60%, showing a strong preference for sweetness, while the preference rate of Comparative Example 1 group was only 35%, which further confirmed that the combination of essential oil and polyphenols in the composition can effectively enhance the pleasure of mice and reduce the degree of depression.

[0066] In summary, chronic unpredictable stress leads to a decrease in the sugar water consumption rate of mice, an increase in the 4-min tail suspension test and the immobility time of swimming, and there are significant differences compared with the blank group (P < 0.05). After the intervention of the composition of Example 1, the sugar water consumption rate increased, and the 4-min tail suspension test and the immobility time of swimming decreased, which were significantly different from the model group (P < 0.05), and there was no significant difference from the blank group, and the effect was equivalent to the fluoxetine positive control group. This shows that after the intervention of the composition of Example 2 and its components, the despair state time of mice was significantly reduced, the anhedonia was relieved, and the behavioral performance tended to normal levels, that is, the depressive-like behavior of mice was significantly improved.

[0067] In order to investigate the effects of different components in the composition of Example 2 on relieving depression symptoms in animals, the following compositions were investigated respectively:

[0068] Composition 1: Daidaihua.

[0069] Composition 2: Buddha's hand.

[0070] Composition 3: black sesame.

[0071] Composition 4: citrus fruit drops.

[0072] Composition 5: Turmeric.

[0073] Composition 6: 20 parts of daidaihua, 15 parts of bergamot, 12 parts of black sesame, 16 parts of citrus fruit drops, and 17 parts of turmeric.

[0074] Composition 7: 13 parts of star anise, 12 parts of fennel, 10 parts of lemon balm, 11 parts of cloves, 7 parts of hawthorn, 6 parts of pumpkin, 6 parts of red beans, 9 parts of walnuts, and 4 parts of wolfberries.

[0075] The above-mentioned compositions were respectively used to prepare essential oil and polyphenol extracts according to the preparation method of Example 2, the essential oil and polyphenol were vortexed for 2 minutes to mix, the composition was embedded in liposomes, and spray-dried into powder. The effects of different components on relieving depression symptoms in animals were investigated, and the results are listed in Table 1.

[0076] Table 1: Effects of different components on relieving depression symptoms in animals

[0077]

[0078] From the results of the investigation in Table 1, it can be seen that compared with the depression model group, daidaihua, citrus hand, black sesame, citrus fruit drops, turmeric and their combinations all have significant antidepressant effects, among which Example 1 has the best antidepressant effect, with the shortest tail suspension immobility time and forced swimming immobility time and the highest sucrose preference rate; while Composition 7 has no significant antidepressant effect, so it is believed that daidaihua, citrus hand, black sesame, citrus fruit drops and turmeric are important ingredients in the combination for relieving depression. Compounds such as volatile components (such as linalool) and polyphenols (such as naringin) contained in the extract of daidaihua produce antidepressant effects by regulating neurotransmitters; polyphenol compounds such as curcumin in turmeric can affect neurotransmitters in the brain, which may affect mood regulation; it has the effect of relieving depressive symptoms, and bergamot has the effect of soothing the liver and regulating qi, and has a certain alleviating effect on symptoms such as liver qi stagnation and chest tightness; the polyphenols in bergamot can regulate liver function and promote the circulation of qi and blood, thereby achieving the effect of soothing the liver and relieving depression; black sesame seeds are rich in unsaturated fatty acids (omega-3 and omega-6), which may affect the synthesis, release and reuptake of serotonin, dopamine and norepinephrine, and these neurotransmitters play a key role in mood regulation such as relieving depression; citrus fruits are rich in polyphenols and have good anti-inflammatory functions, which help resist the deterioration of the nervous system caused by the decomposition of nervous system cells, protect nerves from damage, and help promote brain function. The antidepressant effect of the five-substance combination (composition 6) of essential oils + polyphenols is stronger than that of a single substance (compositions 1 to 5). The combination regulates the liver-brain axis and the level of brain neurotransmitters, thereby alleviating depressive symptoms.

[0079] However, the bioavailability of polyphenols is low, and effective delivery methods are needed to achieve the best effect. Star anise, fennel, lemon balm, cloves, hawthorn, pumpkin, red beans, walnuts, and wolfberries are rich in volatile oils, vitamins, minerals and other nutritional functional ingredients. When combined with daidaihua, bergamot, black sesame, citrus fruit, and turmeric, they can further exert their antidepressant effects.

[0080] Therefore, the composition provided by the present invention achieves the anti-depressant effect through the complementary effects of various components.

[0081] Experiment 2: Examining brain neurotransmitter levels.

[0082] In the mice of each embodiment and comparative example, brain and liver samples were collected for ELISA detection. Using ELISA kits, brain 5-HT and DA levels, as well as liver IL-1β, IL-6, AST and ALT concentrations were detected, and all experimental steps were strictly carried out according to the manufacturer's instructions. In addition, the levels of mouse serum TG and LDL-C were analyzed using a fully automatic biochemical analyzer. Table 2 shows the test results of brain and liver biochemical index detection.

[0083] Table 2: Results of brain and liver biochemical index tests

[0084]

[0085] From the results in Table 2, it can be seen that the 5-HT (5-hydroxytryptamine) and DA (dopamine) levels measured in brain tissues show that each group can significantly increase the neurotransmitter levels in brain tissues, among which the 5-HT and DA levels in Example 2 group are significantly higher than those in other groups, and the neurotransmitter level in Comparative Example 1 group is the lowest. The essential oil and polyphenols in the composition can increase the higher levels of 5-HT and DA, indicating that they may improve the emotional state of mice by synergistically regulating the balance of neurotransmitters and have a good effect in relieving depression.

[0086] In terms of inflammatory factors, each group can significantly reduce the concentration of liver inflammatory factors IL-1β and IL-6, indicating that the various formulas and ingredients (essential oils and polyphenols) of the composition can effectively relieve liver inflammation, among which the concentrations of IL-1β and IL-6 in the liver of mice in Example 2 group were significantly lower than those in other groups, indicating that Example 2 group may have a significant inhibitory effect on the inflammatory response of the liver. In terms of liver function indicators, the AST and ALT levels of Example 2 group were lower than those of Comparative Example 1 group, which further confirmed the effect of Example 2 group in protecting liver function.

[0087] Figure 2 The results are the levels of serum TG and LDL-C in mice. Low levels of TG and LDL-C in serum help reduce the burden on multiple organs such as the brain and liver. The results show that each group can significantly reduce serum TG and LDL-C levels, indicating that the various formulas and ingredients (essential oils and polyphenols) of the composition can effectively reduce blood lipid levels; among them, the TG levels of Example 2 group and Comparative Example 2 group are relatively low, the LDL-C level of Example 2 group is the lowest, and the TG and LDL-C levels of Comparative Example 1 group are the highest. The serum blood lipid results combined with the antidepressant effect of Example 2 group may indicate that the antidepressant effect of Example 2 may be related to its potential metabolic protective effect.

[0088] Experiment 3: Investigate lipid metabolism.

[0089] Collect 100 μL of liver and brain homogenate from mice in each embodiment and comparative example, mix with 80 μL of methanol and 400 μL of methyl tert-butyl ether, and centrifuge the mixture at 15000 rpm for 15 min at 4 ° C. Subsequently, 350 μL of supernatant was transferred to a 1.5 mL EP tube, dried with nitrogen, and 100 μL of extract (isopropanol: acetonitrile = 1: 1) was added, vortexed and mixed for 30 s, and then low-temperature ultrasonic extraction was performed for 5 min. The mixture was then centrifuged at 15000 rpm at 4 ° C. The supernatant was taken and analyzed using LC-MS. Since the composition and its components have a significant protective effect on chronic unpredictable liver damage and can reduce blood lipids, we detected the liver and brain lipid metabolism of Example 1 Group (CG), Example 2 Group (OP) and Depression Group (CUMS), and compared the three groups of metabolites. It is expected to verify from the changes in metabolites that the composition and its components relieve depressive symptoms by regulating the liver-brain axis.

[0090] Figure 3 Heat map of liver lipid metabolism of the composition of Example 1 and the essential oil + polyphenol group in Example 2. Figure 4 The results are that the composition of Example 1 and the essential oil + polyphenol group in Example 2 regulated the liver-brain axis through the lipid metabolism pathway. The results showed that the livers of the Example 1 group and the Example 2 group significantly changed the phospholipid metabolism pathway, including increasing the levels of diglyceride (16:2e / 18:1; 18:3e / 20:1), various triglycerides, various phosphatidyl methanols, phosphatidylcholine (9:0 / 23:0), and phosphatidylethanolamine (16:0p / 18:1; 20:2e / 22:6; 22:4 / 22:6), and reducing the levels of lysophosphatidylethanolamine (19:0; 20:0) and lysophosphatidylcholine. The brain phospholipid metabolism pathway also showed significant changes, with an increase in the content of phosphatidylethanolamine (14:0e / 16:0; 18:2 / 22:6; 8:1e / 10:0), a decrease in the content of phosphatidylethanolamine (30:0 / 20:0; 4:0 / 16:1; 20:3p / 24:0), and an increase in lysophosphatidylcholine (18:2; 16:1) and lysophosphatidylethanolamine (18:1; 16:1e). Therefore, it is speculated that the increased phosphatidylcholine and phosphatidylethanolamine in the liver are transported to the brain through the blood, decomposed into lysophosphatidylcholine and lysophosphatidylethanolamine to exert an antidepressant effect, indicating that the composition and its essential oil combined with polyphenols regulate the liver-brain axis and the level of brain neurotransmitters, thereby alleviating depressive symptoms. Figure 5 Schematic diagram of the composition and its essential oil + polyphenol group regulating the liver-brain axis through the lipid metabolism pathway.

[0091] The above is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as above in the preferred embodiment, it is not used to limit the present invention. Any technician familiar with the art can make many possible changes and modifications to the technical solution of the present invention by using the methods and technical contents disclosed above without departing from the spirit and technical solution of the present invention, or modify it into an equivalent embodiment of equivalent changes. Therefore, any simple modification, equivalent replacement, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention, still fall within the scope of protection of the technical solution of the present invention.

Claims

1. A composition for relieving depression, characterized in that: include: 10-17 parts of star anise, 10-15 parts of fennel, 9-12 parts of lemon balm, 18-25 parts of daidai flower, 8-15 parts of cloves, 12-20 parts of black sesame, 12-16 parts of bergamot, 5-17 parts of hawthorn, 15-20 parts of turmeric, 5-10 parts of pumpkin, 5-10 parts of red beans, 15-20 parts of citrus fruits, 5-17 parts of walnuts, and 4-7 parts of wolfberries.

2. The composition according to claim 1, characterized in that include: 13 parts of star anise, 12 parts of fennel, 10 parts of lemon balm, 20 parts of daidaihua, 11 parts of cloves, 12 parts of black sesame, 15 parts of bergamot, 7 parts of hawthorn, 15-20 parts of turmeric, 6 parts of pumpkin, 6 parts of red beans, 16 parts of citrus fruits, 9 parts of walnuts, and 4 parts of wolfberries.

3. The composition according to claim 1, characterized in that The invention comprises essential oil and polyphenol extracts, wherein the essential oil and polyphenol extracts are extracted from the following raw materials: 10-17 parts of star anise, 10-15 parts of fennel, 9-12 parts of lemon balm, 18-25 parts of daisy flower, 8-15 parts of cloves, 12-20 parts of black sesame, 12-16 parts of bergamot, 5-17 parts of hawthorn, 15-20 parts of turmeric, 5-10 parts of pumpkin, 5-10 parts of red bean, 15-20 parts of citrus fruit drops, 5-17 parts of walnuts and 4-7 parts of wolfberries.

4. A method for preparing the composition according to any one of claims 1 to 3, characterized in that: The preparation method comprises: S1, star anise, fennel, lemon balm, daidai flower, clove, black sesame, bergamot, hawthorn, turmeric, pumpkin, red bean, citrus fruit, walnut and wolfberry are crushed to obtain material 1; S2, distilling the material 1 on water to obtain essential oil and material 2; S3, mixing the material 2 with ethanol, subjecting it to physical field treatment, and then decocting it, and concentrating the filtrate obtained by decompression and drying it to obtain a polyphenol extract; S4, mixing the essential oil and the polyphenol extract, embedding them in liposomes, and spray drying them into powder.

5. The preparation method according to claim 4, characterized in that: In S2, the mass ratio of the material 1 to water is 1:1-6, and the time of the water distillation is 2-5 hours.

6. The preparation method according to claim 4, characterized in that: The physical field treatment in S3 includes one or more of ultrasonic treatment, microwave treatment, and ultra-high pressure treatment; The power of the ultrasonic treatment is 20 to 100 kHz, the temperature is 30 to 90° C., and the treatment time is 10 s to 2 h. The microwave treatment has a power of 50 to 500 W, a temperature of 30 to 90° C., and a treatment time of 10 s to 2 h. The intensity of the ultra-high pressure treatment is 50-800 MPa, the temperature is 30-90° C., and the treatment time is 10 s to 2 h.

7. The preparation method according to claim 4, characterized in that: In S3, the material 2 is mixed with ethanol in a mass ratio of 1:3-10; the concentration of ethanol is 30%-90%.

8. The preparation method according to claim 4, characterized in that: The decoction time in S3 is 2 to 4 hours, the extraction times are 1 to 3 times, the temperature of reduced pressure concentration is 30 to 60° C., and the drying temperature is -40° C. to 80° C.

9. The preparation method according to claim 4, characterized in that: In S4, the essential oil and the polyphenol extract are mixed in a vortex mixer for 10 seconds to 5 minutes, and the mixture of the essential oil and the polyphenol is encapsulated by inclusion technology, nanotechnology and / or microencapsulation technology to prepare particles with a particle size of about 150 to 500 nm.

10. Use of the composition according to any one of claims 1 to 3 in the preparation of food, medicine or health product for regulating depression in the liver-brain axis.

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