Composition, preparation, food and application for enhancing immunity

Abstract

The present invention provides a composition, preparation method, food product, and application thereof, which are related to the field of food and health care technology. The composition comprises the following components: protamine, maca powder, a traditional Chinese medicine extract, raspberry powder, polysaccharide, and collagen; the traditional Chinese medicine extracts are American ginseng, wolfberry, and andrographis paniculata; the mass ratio of American ginseng, wolfberry, and andrographis paniculata is 2-6:2-4:2-4. The composition provided by the present invention, through the specific components, proportions, and preparation method, not only has the efficacy of significantly improving immunity and anti-aging ability, but also has a good taste.

Description

Technical Field

[0001] The present invention relates to the technical field of food health care, and in particular to a composition with the effects of improving immunity and enhancing physical strength, as well as its preparation, food and application. Background Art

[0002] Aging is a natural process, the result of long-term interaction between organisms and the external environment. As we age, our body functions begin to decline, manifested as structural and functional decline, and decreased adaptability and resistance. The more obvious signs of aging include: osteoporosis caused by calcium loss, vascular sclerosis causing cardiovascular and cerebrovascular diseases and stroke, decreased immune function, decreased sexual function, etc. The incidence of cardiovascular and cerebrovascular diseases such as myocardial infarction and cerebral hemorrhage in middle-aged people has increased significantly, and the incidence of diseases such as coronary heart disease, hypertension, hyperlipidemia, and diabetes is also tending to be younger. People are constantly seeking ways to maintain health and longevity, improve immunity, overcome various diseases, and improve physical functions in order to improve their quality of life. Therefore, the continuous development of nutritious and health foods with anti-aging, immunity-enhancing and resistance-enhancing medicinal and edible properties that are easily absorbed and utilized by the human body has become a technical problem that urgently needs to be solved by those skilled in the art.

[0003] Protamine is a polycationic antimicrobial peptide found in fish sperm nuclei. It is a small, simple, globular basic protein with a low molecular weight, typically below 10,000. It is generally composed of around 30 amino acids, rich in proline and arginine, with more than two-thirds being arginine. Its isoelectric point is 10-12, making it soluble in water and dilute acid and non-coagulating upon heating. As a protein rich in arginine, protamine possesses highly nutritious and functional properties, such as lowering blood pressure, aiding respiration, promoting digestion, inhibiting tumors, combating thrombosis, strengthening liver function, and inhibiting blood coagulation. Furthermore, protamine has found widespread medical applications, such as delaying or preventing insulin release and serving as an antidote for heparin. Researchers in this field have studied the effects of protamine in improving immunity and anti-aging.

[0004] For example, "Effects of protamine-coated PLA nanocapsules on the immune function of mouse bone marrow dendritic cells" (Journal of Huazhong University of Science and Technology (Medical Edition), Yang Ping and Zhu Junming, Vol. 45, No. 3, P298) disclosed the effects of protamine (PS)-coated polylactic acid (PLA) nanocapsules on the in vitro immune response of mouse bone marrow dendritic cells (BMDCs). However, this study mainly utilized the property of protamine as a positively charged and biodegradable material, and did not study the ability of protamine as a nutrient itself to enhance immunity.

[0005] Chinese patent application CN108309834A discloses a brown algae extract-based skin care composition, comprising methylsilanol mannuronate, dimethylsilanol, brown algae extract, nano-zinc powder, protamine, beeswax, and water. The brown algae extract-based skin care composition, with the brown algae extract as the primary active ingredient, and particularly when used in combination with protamine, can provide a nutritional supplement to the skin and help repair damaged cells. This demonstrates the application of protamine in the preparation of skin care products with anti-aging and repairing properties, but does not address the use of protamine as a nutrient in the food and health sector to enhance immunity.

[0006] In summary, developing an edible composition with protamine as the main active ingredient, which has significant effects in improving immunity, anti-aging, and strengthening the body, is the research focus of researchers in this field. Summary of the Invention

[0007] In response to the above problems, the present invention provides a composition with the effects of improving immunity and enhancing physical strength. The composition uses protamine as the main component, which works together with maca powder, traditional Chinese medicine extract, raspberry powder, polysaccharide and collagen to prepare a composition with significant effects of improving immunity and anti-aging.

[0008] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0009] On the one hand, the present invention provides a composition with the effect of improving immunity and enhancing physical strength, comprising the following components: protamine, maca powder, traditional Chinese medicine extract, raspberry powder, polysaccharide and collagen; the traditional Chinese medicine extract is American ginseng, wolfberry and andrographis paniculata; the mass ratio of American ginseng, wolfberry and andrographis paniculata is 2-6:2-4:2-4.

[0010] Preferably, the mass ratio of the protamine, raspberry powder and polysaccharide is 8-12:1-3:0.1-2.

[0011] Further preferably, the mass ratio of the protamine, raspberry powder and polysaccharide is 10:2:1.

[0012] Preferably, the mass ratio of American ginseng, wolfberry and Andrographis paniculata is 3-4:2-3:2-3.

[0013] More preferably, the mass ratio of American ginseng, wolfberry and Andrographis paniculata is 4:3:3.

[0014] Preferably, the preparation method of the Chinese herbal medicine extract is:

[0015] Step 1) mixing the raw materials with alcohol, heating and extracting for 1-3 times, combining the extracts to obtain an alcohol extract and a filter residue;

[0016] Step 2) the filter residue is further leached with water for 1-3 times, and the water extract is obtained by combining;

[0017] Step 3) The alcohol extract and the water extract are combined to concentrate the extract, which is then dried and crushed.

[0018] Preferably, in step 1), the material-liquid ratio of the raw material to the alcohol is 1 g:8-12 L.

[0019] Further preferably, in step 1), the material-liquid ratio of the raw material to the alcohol is 1 g:9-10 L; more preferably, in step 1), the material-liquid ratio of the raw material to the alcohol is 1 g:9 L.

[0020] Preferably, in step 1), the alcohol is an ethanol solution with a volume fraction of 60%-75%.

[0021] Further preferably, in step 1), the alcohol is an ethanol solution with a volume fraction of 65%.

[0022] Preferably, in step 2), the water is selected from at least one of purified water, deionized water or distilled water; further preferably, in step 2), the water is purified water.

[0023] Preferably, in step 2), the material-liquid ratio of the filter residue to water is 1 g:8-12 L.

[0024] Further preferably, in step 2), the solid-liquid ratio of the filter residue to water is 1 g:9-10 L; more preferably, in step 2), the solid-liquid ratio of the filter residue to water is 1 g:10 L.

[0025] Preferably, in step 3), the relative density of the extract is 1-1.2.

[0026] Further preferably, the relative density of the extract is 1.

[0027] Preferably, the polysaccharide is Tricholoma matsutake polysaccharide.

[0028] Preferably, the preparation method of the Tricholoma matsutake polysaccharide is:

[0029] The pine mushroom powder is mixed with enzyme and water, and enzymatically hydrolyzed; microwave extraction is performed, and the water extract is freeze-dried to obtain a concentrated product.

[0030] Preferably, the water is selected from at least one of purified water, deionized water or distilled water; further preferably, the water is purified water.

[0031] Preferably, the enzyme is selected from at least one of cellulase and papain; further preferably, the enzyme is a mixture of cellulase and papain.

[0032] Preferably, the dosage of the enzyme is 18-24 U / g of pine mushroom powder.

[0033] More preferably, the dosage of the enzyme is 20 U / g of pine mushroom powder.

[0034] Preferably, the pH of the enzymatic hydrolysis is 5-6 and the temperature is 50-60°C.

[0035] More preferably, the pH of the enzymatic hydrolysis is 5.5 and the temperature is 55°C.

[0036] Preferably, the frequency of the microwave extraction is 32-38 KHz.

[0037] Further preferably, the frequency of the microwave extraction is 34KHz.

[0038] Further preferably, the preparation method of the matsutake polysaccharide is: wash the matsutake, dry it, and crush it to obtain matsutake powder; add cellulase and papain, add water and stir evenly, adjust the pH value to 5-6, and perform enzymolysis at 50-60°C for 20-30 minutes; perform microwave extraction at a frequency of 32-38KHz, and freeze-dry the water extract to obtain a concentrated product.

[0039] Preferably, the composition comprises the following components in parts by mass: 80-120 parts of protamine, 10-20 parts of maca powder, 5-15 parts of traditional Chinese medicine extract, 10-30 parts of raspberry powder, 1-20 parts of polysaccharide and 15-30 parts of collagen.

[0040] Further preferably, the composition comprises the following components in parts by mass: 80-100 parts of protamine, 10-15 parts of maca powder, 10-15 parts of traditional Chinese medicine extract, 20-30 parts of raspberry powder, 1-10 parts of polysaccharide and 15-25 parts of collagen.

[0041] More preferably, the composition comprises the following components in parts by mass: 100 parts of protamine, 15 parts of maca powder, 10 parts of traditional Chinese medicine extract, 20 parts of raspberry powder, 10 parts of polysaccharide and 25 parts of collagen.

[0042] Preferably, the composition further comprises at least one of a flavoring agent or a fragrance.

[0043] Preferably, the flavoring agent is selected from at least one of D-mannitol, alitame, aspartame, advantame, erythrosine, xylitol or sucralose.

[0044] Preferably, the flavor is selected from at least one of sweet orange flavor, vanilla flavor, strawberry flavor, milk flavor, banana flavor or cherry flavor.

[0045] In another aspect, the present invention provides a method for preparing the above-mentioned composition, specifically comprising mixing the components in the composition.

[0046] In another aspect, the present invention provides use of the above-mentioned composition in preparing food having immunity-enhancing and anti-aging effects.

[0047] In another aspect, the present invention provides a food having immunity-enhancing and anti-aging effects, comprising the above-mentioned composition.

[0048] Preferably, the food is in the form of a formulation suitable for oral administration.

[0049] Compared with the prior art, the present invention has the following beneficial effects:

[0050] 1. By optimizing the components and preparation methods of Chinese herbal extracts, the present invention has found in experiments that American ginseng, wolfberry, and Andrographis paniculata have a synergistic effect in improving immunity and anti-aging capabilities, especially under the specific ratio and preparation method of the present invention;

[0051] 2. The present invention compounds protamine, maca powder, Chinese herbal extracts, raspberry powder, pine mushroom polysaccharide, and collagen to obtain a composition that can significantly enhance immunity and anti-aging capabilities. In particular, under the specific ratio of the present invention, protamine, raspberry powder, and pine mushroom polysaccharide have a synergistic effect.

[0052] 3. The composition prepared by the present invention not only has the effect of significantly improving immunity and anti-aging ability, but also has a good taste, which can significantly improve the user's acceptance. DETAILED DESCRIPTION

[0053] In order to make the technical means, creative features, purpose and effect of the present invention easy to understand, the present invention is further illustrated below in conjunction with specific embodiment, but the following embodiment is only a preferred embodiment of the present invention, not all. Based on the embodiment in the embodiment, other embodiments obtained by those skilled in the art without making creative work all fall within the protection scope of the present invention. It is worth noting that the raw materials used in the present invention are all common commercial products, and their source is not specifically limited. The technology and scientific terms used in the embodiment have the meaning commonly understood by those of ordinary skill in the art to which the present invention belongs.

[0054] Example 1

[0055] A composition with the effects of improving immunity and enhancing physical strength comprises the following components: 100 parts of protamine, 15 parts of maca powder, 10 parts of traditional Chinese medicine extract, 20 parts of raspberry powder, 10 parts of pine mushroom polysaccharide, 25 parts of collagen, 3 parts of xylitol, and 2 parts of cherry flavor.

[0056] (1) The Chinese herbal extract is a mixture of American ginseng, wolfberry, and Andrographis paniculata, with a mass ratio of 4:3:3.

[0057] The preparation method of the Chinese herbal medicine extract is as follows:

[0058] Step 1) crushing the raw material, mixing it with a 65% by volume ethanol solution at a material-liquid ratio of 1 g:9 L, heating it to reflux and extracting it three times, combining the extracts to obtain an alcohol extract and a filter residue;

[0059] Step 2) The filter residue was further leached with purified water three times at a solid-liquid ratio of 1 g:10 L, and the aqueous extract was obtained by combining the two solutions;

[0060] Step 3) combining the alcohol extract and the water extract, concentrating the extract, freeze-drying, and pulverizing.

[0061] (2) The preparation method of Matsutake polysaccharide is as follows:

[0062] Wash, dry, and crush the matsutake mushrooms to obtain matsutake powder. Add cellulase and papain (the enzyme dosage is 20 U / g matsutake powder), add purified water and stir evenly, adjust the pH value to 5.5, and perform enzymatic hydrolysis at 55°C for 30 minutes. Perform microwave extraction at a frequency of 34 kHz, and freeze-dry the water extract to obtain a concentrated product.

[0063] (3) The preparation method of the composition having the effects of improving immunity and enhancing physical strength is as follows: the components of the composition are mixed evenly and passed through a 40-100 mesh sieve.

[0064] Example 2

[0065] A composition having the effects of improving immunity and enhancing physical strength, which differs from Example 1 only in the following components:

[0066] It contains the following ingredients: 80 parts of protamine, 20 parts of maca powder, 15 parts of Chinese herbal medicine extract, 30 parts of raspberry powder, 1 part of pine mushroom polysaccharide, 30 parts of collagen, 5 parts of xylitol, and 5 parts of cherry flavor.

[0067] The rest is the same as Example 1.

[0068] Example 3

[0069] A composition having the effects of improving immunity and enhancing physical strength, which differs from Example 1 only in the following components:

[0070] It contains the following ingredients: 120 parts of protamine, 10 parts of maca powder, 5 parts of Chinese herbal medicine extract, 10 parts of raspberry powder, 20 parts of pine mushroom polysaccharide, 15 parts of collagen, 4 parts of xylitol, and 1 part of cherry flavor.

[0071] The rest is the same as Example 1.

[0072] Example 4

[0073] A composition having the effects of improving immunity and enhancing physical strength, which differs from Example 1 only in that the Chinese herbal extract is different:

[0074] The Chinese herbal extract is a mixture of American ginseng, wolfberry and andrographis paniculata, with the mass ratio of the three being 3:2:2.

[0075] The rest is the same as Example 1.

[0076] Comparative Example 1

[0077] A composition having the effects of improving immunity and enhancing physical strength, which differs from Example 1 only in the following components:

[0078] It contains the following ingredients: 130 parts of protamine, 1 part of maca powder, 20 parts of Chinese medicine extract, 12 parts of raspberry powder, 0.5 parts of pine mushroom polysaccharide, 10.5 parts of collagen, 6 parts of xylitol, and 5 parts of cherry flavor.

[0079] The preparation method of the composition having the effects of improving immunity and enhancing physical strength is as follows: the components of the composition are mixed evenly and passed through a 40-100 mesh sieve.

[0080] The rest is the same as Example 1.

[0081] Comparative Example 2

[0082] A composition having the effects of improving immunity and enhancing physical strength, which differs from Example 1 only in the following components:

[0083] 108 parts of protamine, 15 parts of maca powder, 10 parts of Chinese herbal medicine extract, 22 parts of raspberry powder, 25 parts of collagen, 3 parts of xylitol, and 2 parts of cherry flavor.

[0084] The preparation method of the composition having the effects of improving immunity and enhancing physical strength is as follows: the components of the composition are mixed evenly and passed through a 40-100 mesh sieve.

[0085] The rest is the same as Example 1.

[0086] Comparative Example 3

[0087] A composition having the effects of improving immunity and enhancing physical strength, which differs from Example 1 only in the following components:

[0088] It contains the following ingredients: 118 parts of protamine, 15 parts of maca powder, 10 parts of Chinese herbal medicine extract, 12 parts of pine mushroom polysaccharide, 25 parts of collagen, 3 parts of xylitol, and 2 parts of cherry flavor.

[0089] The preparation method of the composition having the effects of improving immunity and enhancing physical strength is as follows: the components of the composition are mixed evenly and passed through a 40-100 mesh sieve.

[0090] The rest is the same as Example 1.

[0091] Comparative Example 4

[0092] A composition having the effects of improving immunity and enhancing physical strength, which differs from Example 1 only in the following components:

[0093] 100 parts of casein, 15 parts of maca powder, 10 parts of Chinese herbal medicine extract, 20 parts of raspberry powder, 10 parts of pine mushroom polysaccharide, 25 parts of collagen, 3 parts of xylitol, and 2 parts of cherry flavor.

[0094] The preparation method of the composition having the effects of improving immunity and enhancing physical strength is as follows: the components of the composition are mixed evenly and passed through a 40-100 mesh sieve.

[0095] The rest is the same as Example 1.

[0096] Comparative Example 5

[0097] A composition having the effects of improving immunity and enhancing physical strength, which differs from Example 1 only in the preparation method of Tricholoma matsutake polysaccharide:

[0098] Wash, dry and crush the matsutake mushrooms to obtain matsutake mushroom powder; add 15 times the mass of water, mix, heat to 90°C, soak for 3 hours, filter the extract, add 3 times the volume of 95% ethanol, and precipitate for 3 hours to obtain matsutake mushroom polysaccharide.

[0099] The rest is the same as Example 1.

[0100] Comparative Example 6

[0101] A composition having the effects of improving immunity and enhancing physical strength, the only difference from Example 1 being that the polysaccharide from Tricholoma matsutake is replaced with polysaccharide from Ganoderma lucidum:

[0102] The preparation method of the composition having the effects of improving immunity and enhancing physical strength is as follows: the components of the composition are mixed evenly and passed through a 40-100 mesh sieve.

[0103] The rest is the same as Example 1.

[0104] Comparative Example 7

[0105] A composition having the effects of improving immunity and enhancing physical strength, which differs from Example 1 only in that the Chinese herbal extract is different:

[0106] (1) The Chinese herbal extract is a mixture of American ginseng, wolfberry, and Andrographis paniculata, with a mass ratio of 2:5:5.

[0107] The preparation method of the Chinese herbal medicine extract is as follows:

[0108] Step 1) crushing the raw material, mixing it with a 65% by volume ethanol solution at a material-liquid ratio of 1 g:9 L, heating it to reflux and extracting it three times, combining the extracts to obtain an alcohol extract and a filter residue;

[0109] Step 2) The filter residue was further leached with purified water three times at a solid-liquid ratio of 1 g:10 L, and the aqueous extract was obtained by combining the two solutions;

[0110] Step 3) combining the alcohol extract and the water extract, concentrating the extract, freeze-drying, and pulverizing.

[0111] The rest is the same as Example 1.

[0112] Comparative Example 8

[0113] A composition having the effects of improving immunity and enhancing physical strength, which differs from Example 1 only in that the Chinese herbal extract is different:

[0114] (1) The Chinese herbal medicine extract is American ginseng.

[0115] The preparation method of the Chinese herbal medicine extract is as follows:

[0116] Step 1) crushing the raw material, mixing it with a 65% by volume ethanol solution at a material-liquid ratio of 1 g:9 L, heating it to reflux and extracting it three times, combining the extracts to obtain an alcohol extract and a filter residue;

[0117] Step 2) The filter residue was further leached with purified water three times at a solid-liquid ratio of 1 g:10 L, and the aqueous extract was obtained by combining the two solutions;

[0118] Step 3) combining the alcohol extract and the water extract, concentrating the extract, freeze-drying, and pulverizing.

[0119] The rest is the same as Example 1.

[0120] Comparative Example 9

[0121] A composition having the effects of improving immunity and enhancing physical strength, which differs from Example 1 only in that the Chinese herbal extract is different:

[0122] (1) The Chinese herbal extract is Andrographis paniculata.

[0123] The preparation method of the Chinese herbal medicine extract is as follows:

[0124] Step 1) crushing the raw material, mixing it with a 65% by volume ethanol solution at a material-liquid ratio of 1 g:9 L, heating it to reflux and extracting it three times, combining the extracts to obtain an alcohol extract and a filter residue;

[0125] Step 2) The filter residue was further leached with purified water three times at a solid-liquid ratio of 1 g:10 L, and the aqueous extract was obtained by combining the two solutions;

[0126] Step 3) combining the alcohol extract and the water extract, concentrating the extract, freeze-drying, and pulverizing.

[0127] The rest is the same as Example 1.

[0128] Comparative Example 10

[0129] A composition having the effects of improving immunity and enhancing physical strength, which differs from Example 1 only in that the Chinese herbal extract is different:

[0130] (1) The Chinese herbal extract is wolfberry.

[0131] The preparation method of the Chinese herbal medicine extract is as follows:

[0132] Step 1) crushing the raw material, mixing it with a 65% by volume ethanol solution at a material-liquid ratio of 1 g:9 L, heating it to reflux and extracting it three times, combining the extracts to obtain an alcohol extract and a filter residue;

[0133] Step 2) The filter residue was further leached with purified water three times at a solid-liquid ratio of 1 g:10 L, and the aqueous extract was obtained by combining the two solutions;

[0134] Step 3) combining the alcohol extract and the water extract, concentrating the extract, freeze-drying, and pulverizing.

[0135] The rest is the same as Example 1.

[0136] Comparative Example 11

[0137] A composition having the effects of improving immunity and enhancing physical strength, which differs from Example 1 only in that the Chinese herbal extract is different:

[0138] (1) The Chinese herbal extract is a mixture of American ginseng, wolfberry, and Andrographis paniculata, with a mass ratio of 4:3:3.

[0139] The preparation method of the Chinese medicine extract is as follows: uniformly mixing the above raw materials.

[0140] Test Example 1

[0141] Immunity effect test:

[0142] Experimental animals: 12-16 g female BALB / c young mice, provided by Chengdu Dashuo Biotechnology Co., Ltd., were randomly divided into 15 groups, 10 mice in each group, namely, Example groups (Examples 1 to 4), Comparative Example groups (Comparative Example 1 to Comparative Example 11), and a control group;

[0143] Experimental Design:

[0144] Example Group: 1.5 g of each composition prepared in Example 1 to Example 4 was dissolved in 200 mL of warm water.

[0145] Comparative Example Group: 1.5 g of each composition prepared in Comparative Example 1 to Comparative Example 11 was dissolved in 200 mL of warm water.

[0146] Control group: administered with equal dose of normal saline.

[0147] Dosage: Each mouse was gavaged once with 0.1 mL / g twice a day for 5 consecutive weeks. The mice were sacrificed 4 hours after the last gavage, and the immune organs were isolated and weighed. The following analysis results were obtained, as shown in Table 1.

[0148] Detection indicators: mouse weight gain, thymus / body weight ratio, spleen / body weight ratio.

[0149] Table 1. Results of the Immunity Effect Test

[0150]

[0151] Note: *P<0.05, **P<0.01 compared with the control group.

[0152] As can be seen from the data in Table 1, the composition prepared in the examples of the present invention is safe and has no toxic side effects when administered to mice, can significantly stimulate the growth of the immune organs of mice, and has a good effect of enhancing immune function. Compared with the comparative example, the efficacy is obvious, indicating that only under the specific components, formula and preparation method of the present invention can the effect of significantly enhancing immunity be achieved.

[0153] Test Example 2

[0154] Anti-aging test

[0155] Experimental animals:

[0156] Kunming mice aged 12 months or older (aged mice), weighing 30-50 g, were randomly divided into 15 groups, 10 mice in each group, including Example groups (Examples 1 to 4), Comparative Example groups (Comparative Example 1 to Comparative Example 11), and a control group;

[0157] Experimental Design:

[0158] Example group: The rats were fed with the compositions prepared in Examples 1 to 4, 3 times a day, 100 mg each time, for 45 consecutive days.

[0159] Comparative Example Group: The rats were fed with the compositions prepared in Comparative Example 1 to Comparative Example 11, 3 times a day, 100 mg each time, for 45 consecutive days.

[0160] Control group: administered with equal dose of normal saline.

[0161] Detection:

[0162] After 45 days, the erythrocyte superoxide dismutase (SOD) activity, heart and liver SOD activity, and serum oxidized lipid (LPO) levels were measured in the mice from the control group, Examples 1-4, and Comparative Examples 1-11. Erythrocyte SOD activity was measured using the pyrogallol method, heart and liver SOD activity was measured using the Lanstein method, and serum oxidized lipid (LPO) levels were measured using a modified Yagi method. Specific test results are shown in Table 2.

[0163] Table 2. Anti-aging test results

[0164]

[0165] Note: *P<0.05, **P<0.01 compared with the control group.

[0166] As can be seen from the data in Table 2, the composition prepared in the embodiment of the present invention is safe and has no toxic side effects when fed to mice, can significantly enhance the SOD activity of mice, reduce the LPO content, and has the effect of delaying aging. Compared with the comparative example, the efficacy is obvious, indicating that only under the specific components, formula and preparation method of the present invention can a significantly better anti-aging effect be achieved.

[0167] Test Example 3

[0168] Taste test:

[0169] Taste test of Examples 1-4 and Comparative Examples 1-11. The specific testing method is to select 150 people, randomly divide them into 15 groups, with 10 people in each group, and let them eat the compositions prepared according to Examples 1-4 and Comparative Examples 1-11, and evaluate and score the taste of the compositions, with a total score of 10 points.

[0170] 0 = no taste, 1 = slightly taste / mouthfeel, 2 = very weak taste / mouthfeel, 3 = weak taste / mouthfeel, 4 = relatively weak taste / mouthfeel, 5 = moderate taste / mouthfeel, 6 = slightly better taste / mouthfeel, 7 = better taste / mouthfeel, 8 = good taste / mouthfeel, 9 = very good taste / mouthfeel, and 10 = very good taste / mouthfeel.

[0171] The scale includes the ability to use 0.1 points, thus having the possibility of 100 level differences.

[0172] The results are shown in Table 3.

[0173] Table 3. Taste test results

[0174]

[0175] As can be seen from the data in Table 3, the compositions prepared in Examples 1 to 4 of the present invention have a good taste. Despite the addition of the Chinese herbal extract, the taste is not adversely affected. By optimizing the preparation method of the Chinese herbal extract, the composition and ratio of the composition, and especially the addition of raspberry powder, the taste is significantly improved, and user acceptance is increased.

[0176] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A composition having the effect of enhancing immunity, characterized in that: The invention comprises the following components: protamine, maca powder, Chinese herbal extract, raspberry powder, polysaccharide and collagen; the Chinese herbal extract is American ginseng, wolfberry and andrographis paniculata; the mass ratio of American ginseng, wolfberry and andrographis paniculata is 2-6:2-4:2-4; The preparation method of the Chinese medicine extract is: Step 1) mixing the raw materials with alcohol, heating and extracting for 1-3 times, combining the extracts to obtain an alcohol extract and a filter residue; Step 2) the filter residue is further leached with water for 1-3 times, and the water extract is obtained by combining; Step 3) combining the alcohol extract and the water extract to concentrate the extract, drying, and crushing; The polysaccharide is Tricholoma matsutake polysaccharide; the preparation method of the Tricholoma matsutake polysaccharide is: mixing Tricholoma matsutake powder with enzyme and water, and performing enzymolysis; Microwave extraction is performed, and the water extract is freeze-dried to obtain a concentrated product; the enzyme is a mixture of cellulase and papain; The composition comprises the following components in parts by mass: 80-120 parts of protamine, 10-20 parts of maca powder, 5-15 parts of traditional Chinese medicine extract, 10-30 parts of raspberry powder, 1-20 parts of polysaccharide and 15-30 parts of collagen.

2. The composition according to claim 1, characterized in that The mass ratio of the protamine, raspberry powder and polysaccharide is 10:2:

1.

3. The composition according to claim 1, characterized in that The mass ratio of American ginseng, wolfberry and andrographis paniculata is 3-4:2-3:2-3.

4. The composition according to claim 1, characterized in that In step 1), the material-liquid ratio of the raw material to the alcohol is 1 g:8-12 L.

5. The composition according to claim 1, characterized in that In step 2), the material-liquid ratio of the filter residue to water is 1g:8-12L.

6. The composition according to claim 1, characterized in that The dosage of the enzyme is 18-24 U / g of pine mushroom powder.

7. The composition according to claim 1, characterized in that The pH of the enzymatic hydrolysis is 5-6, and the temperature is 50-60°C.

8. The composition according to claim 1, characterized in that The composition comprises the following components in parts by mass: 80-100 parts of protamine, 10-15 parts of maca powder, 10-15 parts of traditional Chinese medicine extract, 20-30 parts of raspberry powder, 1-10 parts of polysaccharide and 15-25 parts of collagen.

9. The composition according to claim 1, characterized in that The composition further comprises at least one of a flavoring agent or a fragrance.

10. The method for preparing the composition according to any one of claims 1 to 9, characterized in that: Specifically, the components of the composition are mixed.

11. Use of the composition according to any one of claims 1 to 9 in preparing food having the effect of enhancing immunity.

12. A food having the effect of enhancing immunity, characterized in that: Comprising the composition according to any one of claims 1 to 9.

13. The food according to claim 12, characterized in that The dosage form of the food is in the form of a formulation suitable for oral administration.

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