Acne removing composition for blocking acne marks and application and cosmetics thereof

Abstract

The invention relates to the field of cosmetics, in particular to an acne-removing composition for blocking acne marks, application of the acne-removing composition and cosmetics. The composition comprises the following components in parts by mass: 0.5-2.5 parts of maltose acid, 0.2-1.5 parts of arginine and 1-10 parts of a Chinese herbaceous peony root extract. Maltose acid can relieve inflammatory response, reduce infiltration of inflammatory cells around hair follicles and inhibit release of inflammatory mediators such as interleukin; arginine can improve skin blood circulation; the acne is caused by blocking of hair follicle sebaceous gland ducts and unsmooth blood circulation. NO generated by arginine can relax blood vessels and increase blood perfusion of skin. This contributes to the delivery of nutrients and immune cells to the skin inflammation site. The Chinese herbaceous peony root extract can regulate inflammation-related signal channels and reduce the generation of inflammatory factors; therefore, the composition can be further synergistic with arginine and maltose acid, and acne marks are reduced.

Description

Technical Field

[0001] The present application relates to the field of cosmetics, and in particular, to an anti-acne composition for preventing acne marks and its application and cosmetics. Background Art

[0002] As people pay more attention to skin health and beauty, the market size of acne and acne scar removal products continues to expand, and the technology has also gone through several stages of development.

[0003] Early products mostly used natural herbs, such as tea tree essential oil, which relieved mild acne with its anti-inflammatory and antibacterial properties, but was not very effective for moderate to severe acne. Later, chemical synthetic ingredients became mainstream, such as salicylic acid and fruit acid, which can regulate keratin metabolism and unclog pores, but are highly irritating, especially to sensitive skin, which limits their application. In terms of acne scar removal, currently whitening ingredients such as vitamin C and its derivatives are usually relied on to inhibit melanin synthesis. However, these have limited effects on deeper acne scars. Therefore, existing products have the problem of irritating sensitive skin and failing to comprehensively and efficiently remove acne and acne scars. Summary of the invention

[0004] The purpose of the embodiments of the present application is to provide an acne-removing composition and its application and cosmetics for preventing acne scars.

[0005] In a first aspect, the present application provides an acne-removing composition for preventing acne scars, and the acne-removing composition for preventing acne scars comprises, by weight:

[0006] Maltobionic acid 0.5-2.5, arginine 0.2-1.5, peony root extract 1-10.

[0007] In the above technical scheme, by compounding maltobionic acid, arginine, and peony root extract, a synergistic effect is achieved, which effectively improves the effect of removing acne and acne marks. The three ingredients work synergistically, which greatly enhances the effect of removing acne and acne marks by reducing the conduction of TNF-α inflammatory factors, reducing the impact on blood vessels and melanocytes, inhibiting the deterioration of acne, and preventing the formation of acne marks. Applying this composition to cosmetics is conducive to solving the problem that existing products are irritating to sensitive skin and cannot comprehensively and efficiently remove acne and acne marks.

[0008] Furthermore, the maltobionic acid in the above composition can reduce the inflammatory response, it can reduce the infiltration of inflammatory cells around the hair follicles, and inhibit the release of inflammatory mediators such as interleukins (IL-1β, IL-6, etc.); arginine can improve skin blood circulation; and the occurrence of acne is partly due to the blockage of the sebaceous gland ducts of the hair follicles and poor blood circulation. The NO produced by arginine can dilate blood vessels and increase blood perfusion of the skin. This helps to transport nutrients and immune cells to the site of skin inflammation. Compounding arginine with maltobionic acid can achieve a synergistic effect, accelerate the reduction of TNF-α inflammatory factor conduction, and greatly reduce acne marks. Peony root extract can regulate inflammation-related signaling pathways and reduce the production of inflammatory factors; thereby further synergizing with arginine and maltobionic acid to enhance the effects of acne removal and acne mark removal from different targets.

[0009] In other embodiments of the present application, the anti-acne composition for preventing acne marks includes, by weight:

[0010] Maltobionic acid 1.0-2.0, arginine 0.40-1.0, peony root extract 5-8.

[0011] In other embodiments of the present application, in the anti-acne composition for preventing acne scars, the mass ratio of maltobionic acid to arginine is (2-5):1.

[0012] In other embodiments of the present application, in the anti-acne composition for preventing acne scars, the mass ratio of maltobionic acid to arginine is (2.5-4):1.

[0013] In other embodiments of the present application, the pH value of the acne-removing composition for preventing acne scars is 3.0-5.5.

[0014] In other embodiments of the present application, the pH value of the anti-acne composition for preventing acne marks is 4.0-5.5.

[0015] In other embodiments of the present application, the optical rotation of arginine is in the range of 26.0° to 27.9°.

[0016] In other embodiments of the present application, the peony root extract is added to the composition in the form of a solution.

[0017] In other embodiments of the present application, in the peony root extract solution, the peony root extract contains ≥ 8% by mass.

[0018] In other embodiments of the present application, in the peony root extract, the effective substance of paeoniflorin is ≥ 1% by mass.

[0019] In other embodiments of the present application, the peony root extract solution comprises peony root extract and a solvent; optionally, the solvent comprises butylene glycol, water, and 1,2-hexanediol.

[0020] In a second aspect, the present application provides an application of an anti-acne composition for blocking acne scars in the preparation of cosmetics that inhibit TNF-a inflammatory factors in cells.

[0021] In other embodiments of the present application, the volume concentration of the acne-blocking composition for preventing acne marks in the aqueous solution of the acne-blocking composition for preventing acne marks is 0.0032%-2% by volume.

[0022] In a third aspect, the present application provides a cosmetic comprising the anti-acne composition for preventing acne scars provided in any one of the first aspects.

[0023] In other embodiments of the present application, the amount of maltobionic acid added to the cosmetic is 0.5%-2.5% by mass.

[0024] In other embodiments of the present application, the amount of arginine added to the cosmetic is 0.2%-1.5% by mass.

[0025] In other embodiments of the present application, the amount of peony root extract added to the cosmetic is 1%-10% by mass. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0027] Figure 1 This is a picture of the skin condition of the test subject before using the sample of Example 2;

[0028] Figure 2 This is a picture of the skin condition of the test subject after using the sample of Example 2 for 3 days;

[0029] Figure 3 This is a picture of the skin condition of the test subject before using the sample of Example 2;

[0030] Figure 4 This is a picture of the skin condition of the test subject after using the sample of Example 2 for 3 days;

[0031] Figure 5 It is the ITA° value tested and analyzed by VISA imaging after the subjects used the essence of Example 2 and the commercially available competitor for 7 days;

[0032] Figure 6The L* value was measured and analyzed by VISA imaging after the subjects used the essence of Example 2 and the commercial competitor for 7 days;

[0033] Figure 7 It is the a* value tested and analyzed by VISA imaging after the subjects used the essence of Example 2 and the commercially available competitor for 7 days;

[0034] Figure 8 It is a radar chart of sensory evaluation test of the sample in Example 2 and a commercially available product. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments.

[0036] Therefore, the following detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present application.

[0037] After research, it was found that the mechanism of acne formation is complex, and inflammation plays a key role in its development and the formation of acne scars. The activation of inflammatory factors will cause vasodilation, change blood circulation, increase vascular permeability, and stimulate melanocytes to produce a large amount of melanin, which will then form acne scars. Further research found that the key factor is to reduce the conduction of TNF-α inflammatory factors during the acne formation process, reduce the impact on blood vessels and melanocytes, inhibit the deterioration of acne, and prevent the formation of acne scars. This path of action provides a direction for solving the problems of existing products irritating sensitive skin and lack of comprehensive, efficient and gentle acne scar removal solutions, and also lays the foundation for the development of new acne scar removal compositions.

[0038] Based on this, the embodiment of the present application provides an acne-removing composition for preventing acne marks, and the acne-removing composition for preventing acne marks comprises, by weight:

[0039] Maltobionic acid 0.5-2.5, arginine 0.2-1.5, peony root extract 1-10.

[0040] In the above technical scheme, by compounding maltobionic acid, arginine, and peony root extract, a synergistic effect is achieved, which effectively improves the effect of removing acne and acne marks. The three ingredients work synergistically, which greatly enhances the effect of removing acne and acne marks by reducing the conduction of TNF-α inflammatory factors, reducing the impact on blood vessels and melanocytes, inhibiting the deterioration of acne, and preventing the formation of acne marks. Applying this composition to cosmetics is conducive to solving the problem that existing products are irritating to sensitive skin and cannot comprehensively and efficiently remove acne and acne marks.

[0041] Further optionally, exemplarily, in some embodiments of the present application, the acne-blocking acne-marking acne-removing composition includes, by weight: maltobionic acid 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5 or the range between any two of the foregoing values; arginine 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5 or the range between any two of the foregoing values; peony root extract 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or the range between any two of the foregoing values.

[0042] In the above technical scheme, by compounding maltobionic acid, arginine, and peony root extract, a synergistic effect is achieved, and the effect of removing acne marks is effectively improved. The maltobionic acid in the above composition is easily soluble in water, the aqueous solution is clear and transparent, and the pH value (10% aqueous solution) is 1.0-3.0. It has good moisturizing, antibacterial and mild promotion of keratin renewal and skin metabolism. It can be compounded with other ingredients to prevent excessive degradation of the extracellular matrix around the hair follicles by regulating skin metabolism and inflammation, maintain the integrity of the hair follicle structure, help reduce skin damage caused by acne, and reduce the production of acne marks. At the same time, the occurrence of acne is closely related to inflammation. Inflammation can cause the sebaceous glands to secrete too much sebum, and can also cause inflammatory reactions in the tissues around the hair follicles. The antioxidant effect of maltobionic acid can alleviate inflammatory reactions. It can reduce the infiltration of inflammatory cells around hair follicles and inhibit the release of inflammatory mediators such as interleukins (IL-1β, IL-6, etc.). Maltobionic acid can also affect the pH and has a certain antibacterial effect. It has an inhibitory effect on acne-causing pathogens such as Propionibacterium acnes, and has the effect of removing acne from multiple aspects.

[0043] Furthermore, the arginine in the above composition has a pH value (10% aqueous solution) of 10.0-12.0, has the function of moisturizing and regulating pH, can provide nutrition for skin cells, promote cell metabolism, and help repair damaged skin tissue. This is helpful for improving the texture of the skin, reducing fine lines and wrinkles, and making the skin firmer and smoother. The effect of arginine is reflected in its improvement of skin blood circulation. The occurrence of acne is partly due to the blockage of the sebaceous gland ducts of the hair follicles and poor blood circulation. The NO produced by arginine can dilate blood vessels and increase blood perfusion of the skin. This helps to transport nutrients and immune cells to the inflamed parts of the skin, accelerate the disappearance of inflammation, and greatly reduce acne marks. The above technical scheme, by compounding arginine with other ingredients, can be compounded with maltobionic acid to adjust the pH, improve the antibacterial effect, and enhance the effect of inhibiting inflammatory mediators.

[0044] Further, the peony root extract in the above composition has a pH value of 5.0-7.5 for 1% aqueous solution, and has skin care effects such as moisturizing, anti-oxidation, and skin lightening. When its concentration is 2%, the inhibition rate of hyaluronidase activity is 21.61±1.56%. The peony root extract can inhibit the activation of inflammatory cells such as macrophages and neutrophils, and reduce the release of inflammatory mediators such as prostaglandin E2 (PGE2), histamine, etc. The peony root extract contains high active ingredients, such as the active ingredient paeoniflorin. Peony glycosides can regulate inflammation-related signaling pathways, such as the JAK-STAT (Janus kinase-signal transducer and transcription activator) pathway. Under inflammatory stimulation, the JAK-STAT pathway is activated, which leads to the expression of a series of inflammatory factors, including factors that can induce TNF-α. The peony root extract reduces the production of inflammatory factors by inhibiting the over-activation of the JAK-STAT pathway, thereby indirectly reducing the production of acne marks. The above-mentioned peony root extract can achieve a synergistic effect with arginine and maltobionic acid, and enhance the acne removal effect from different targets.

[0045] Further optionally, in some embodiments of the present application, the anti-acne composition for blocking acne marks comprises, by weight:

[0046] Maltobionic acid 1.0-2.0, arginine 0.40-1.0, peony root extract 5-8.

[0047] Furthermore, in some embodiments of the present application, in the anti-acne composition for preventing acne scars, the mass ratio of maltobionate to arginine is (2-5):1.

[0048] Illustratively, in some embodiments of the present application, in the anti-acne composition for blocking acne scars, the mass ratio of maltobionate to arginine is 2:1, 2.2:1, 2.3:1, 2.4:1, 2.5:1, 2.6:1, 2.7:1, 2.8:1, 2.9:1, 3:1, 3.2:1, 3.5:1, 3.8:1, 4:1, 4.2:1, 4.5:1, 4.8:1, 5:1 or a range between any two of the foregoing ratios.

[0049] In the above technical solution, by setting the mass ratio of maltobionic acid and arginine in the acne-blocking acne-mark composition to (2-5):1, the pH value of the entire acne-blocking acne-blocking composition can be made to be 1.0-3.0; within this range, it is more conducive to blocking acne marks.

[0050] Further optionally, in some embodiments of the present application, in the anti-acne composition for preventing acne scars, the mass ratio of maltobionic acid to arginine is (2.5-4):1.

[0051] Further optionally, in some embodiments of the present application, maltobionate and arginine are added to the composition in the form of powder.

[0052] Further optionally, in some embodiments of the present application, the peony root extract is added to the composition in the form of a solution.

[0053] Further optionally, in some embodiments of the present application, the peony root extract solution comprises peony root extract and a solvent.

[0054] Further optionally, in some embodiments of the present application, the above-mentioned solvent includes butanediol, water, and 1,2-hexanediol.

[0055] Illustratively, in some embodiments of the present application, the solvent includes butanediol, water, and 1,2-hexanediol.

[0056] For example, in some embodiments of the present application, the composition of the peony root extract solution is: peony root extract, butylene glycol, water and 1,2-hexanediol. The above solvents can be mixed in any proportion.

[0057] In terms of mass percentage, the above technical solution contains peony root extract ≥ 8%, and the remaining components are butylene glycol, hexylene glycol and aqueous solution. In the above technical solution, in the peony root extract, the effective substance of paeoniflorin is ≥ 1%. Exemplarily, in terms of mass percentage, in some embodiments of the present application, the peony root extract solution contains 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20% of peony root extract or the range between any two of the foregoing values.

[0058] Illustratively, in some embodiments of the present application, in the peony root extract, the effective substance of paeoniflorin is 1%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0% or a range between any two of the foregoing values.

[0059] Furthermore, in some embodiments of the present application, the pH value of the acne-removing composition for preventing acne scars is 3.0-5.5.

[0060] Low pH (3.0-5.5) can enhance the antibacterial activity of maltobactone and inhibit Propionibacterium acnes; at the same time, the acidic environment helps promote keratin metabolism and unclog pores; in the above technical solution, the pH value of the acne-blocking composition is 3.0-5.5; within this range, it is beneficial to block acne marks.

[0061] Illustratively, in some embodiments of the present application, the pH value of the anti-acne composition for blocking acne marks is 3.0, 3.1, 3.2, 3.5, 3.8, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5 or a range between any two of the foregoing values.

[0062] Further optionally, in some embodiments of the present application, the pH value of the acne-blocking acne-mark removing composition is 4.0-5.5.

[0063] Low pH can enhance the antibacterial activity of maltobactin and inhibit Propionibacterium acnes; at the same time, the acidic environment helps promote keratin metabolism and unclog pores; under neutral to weakly acidic conditions, the stability of paeoniflorin can be enhanced, and it can be compounded with arginine to avoid acid-base neutralization leading to inactivation of the components. However, excessive arginine may cause the pH to rise, weakening the antibacterial effect of maltobactin, and the ratio needs to be controlled. Taking the above into consideration, the above technical solution optimally controls the pH value of the acne-blocking composition to be 4.0-5.5. In this preferred pH range, the composition greatly enhances (1) antibacterial properties: the acidic environment inhibits the growth of Propionibacterium acnes (lower minimum inhibitory concentration MIC value); (2) keratin metabolism: promotes the shedding of old keratin and reduces hair follicle blockage. (3) Stability: Arginine and maltobactin can form ion pairs in this pH range to enhance transdermal absorption. It can greatly improve the efficacy of removing acne and blocking acne.

[0064] Furthermore, in some embodiments of the present application, the optical rotation of arginine ranges from 26.0° to 27.9°.

[0065] In the above technical solution, the optical rotation range of arginine is 26.0° to 27.9°; when it is compounded with other ingredients, it is beneficial to the acne scar removal effect of the composition.

[0066] Illustratively, in some embodiments of the present application, the optical rotation range of arginine is 26.0°, 26.1°, 26.2°, 26.3°, 26.4°, 26.5°, 26.6°, 26.7°, 26.8°, 26.9°, 27.0°, 27.1°, 27.2°, 27.3°, 27.4°, 27.5°, 27.6°, 27.7°, 27.8°, 27.9° or a range between any two of the foregoing values.

[0067] Some embodiments of the present application provide an application of an anti-acne composition for blocking acne scars in the preparation of cosmetics that inhibit TNF-a inflammatory factors in cells.

[0068] Furthermore, in some embodiments of the present application, the volume concentration of the anti-acne composition for blocking acne marks in the aqueous solution of the anti-acne composition for blocking acne marks is 0.0032%-2% by volume.

[0069] Illustratively, in some embodiments of the present application, the volume concentration of the acne-blocking composition for preventing acne scars in the aqueous solution of the acne-blocking composition for preventing acne scars is 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0038%, 0.0040%, 0.0042%, 0.0045%, 0.0048%, 0.0050%, 0.0052%, 0.0055%, 0.01%, 0.05%, 0.1%, 0.5%, 1%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2% or a range between any two of the foregoing values, by volume percentage.

[0070] Some embodiments of the present application provide a cosmetic, comprising an acne-removing composition for preventing acne scars provided in any of the aforementioned embodiments.

[0071] Furthermore, in some embodiments of the present application, the amount of maltobionic acid added to the cosmetic is 0.5%-2.5% by mass.

[0072] Illustratively, in some embodiments of the present application, in terms of mass percentage, the amount of maltobionic acid added to the cosmetic is 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5% or a range between any two of the foregoing values.

[0073] Further optionally, in some embodiments of the present application, the amount of arginine added to the cosmetic is 0.2%-1.5% by mass.

[0074] Illustratively, in some embodiments of the present application, the amount of arginine added to the cosmetic is 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5% or a range between any two of the foregoing values, measured by mass percentage.

[0075] Further optionally, in some embodiments of the present application, the amount of peony root extract added to the cosmetic is 1%-10% by mass.

[0076] Illustratively, in some embodiments of the present application, the amount of peony root extract added to the cosmetic is, by mass percentage, 1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 2.0%, 2.5%, 3.0%, 3.5%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9.0%, 9.5%, 10% or a range between any two of the foregoing values.

[0077] Further optionally, in some embodiments of the present application, the maltobionic acid and arginine in the above-mentioned cosmetics are added to the cosmetics in the form of powder.

[0078] Further optionally, in some embodiments of the present application, the peony root extract in the above-mentioned cosmetics is added to the cosmetics in the form of a solution.

[0079] For example, in some embodiments of the present application, the peony root extract solution is composed of peony root extract, butylene glycol, water, and 1,2-hexanediol. Optionally, in the peony root extract solution, the mass percentage of peony root extract is greater than 80%.

[0080] It should be noted that in the aforementioned cosmetics, the added amount of peony root extract is 1%-10%, which is calculated based on the content of peony root extract; for example, when it is added to cosmetics in the form of a peony root extract solution with a mass percentage of 80%, the mass percentage of the added peony root extract is 1.25% to 12.5%.

[0081] Further optionally, in some embodiments of the present application, the above-mentioned cosmetics also include: mandelic acid, niacinamide, butylene glycol and 10-hydroxydecanoic acid, salicylic acid and betaine, sodium hydroxide, caprylhydroxamic acid and 1,3-propylene glycol and 1,2-hexanediol, xanthan gum, PEG-40 hydrogenated castor oil, allantoin, Melaleuca alternifolia leaf oil, disodium EDTA, and water.

[0082] Further optionally, in some embodiments of the present application, the above-mentioned cosmetics also include: 1%-5% mandelic acid, 1%-5% niacinamide, 1%-5% butylene glycol and 10-hydroxydecanoic acid, 1%-5% salicylic acid and betaine, 0.5%-3% sodium hydroxide, 0.5%-5% caprylhydroxamic acid and 1,3-propylene glycol and 1,2-hexanediol, 0.1%-1.0% xanthan gum, 0.1%-5% PEG-40 hydrogenated castor oil, 0.01%-0.50% allantoin, 0.01%-0.10% Melaleuca alternifolia leaf oil, 0.01%-0.50% disodium EDTA and the remainder water.

[0083] The features and performance of the present application are further described in detail below in conjunction with the embodiments:

[0084] Examples and Comparative Examples

[0085] Provided is an acne-removing composition for preventing acne scars, the composition of which is shown in Table 1.

[0086] Table 1

[0087]

[0088]

[0089] Experimental example

[0090] (1) Treatment of test samples

[0091] Anti-acne composition: dilute each example or comparative example sample with pure water to a solution with a volume fraction of 2%, and then filter with a 0.22 μm filter to collect the filtrate as the sample mother solution.

[0092] Negative control: basic culture medium (cell activity test); basic culture medium containing LPS (TNF-a inflammatory factor content determination).

[0093] (2) Test operation steps

[0094] Cell activity test: Take RAW264.7 cells and spread them on a 96-well plate. After 24 hours, aspirate the culture medium and add the basic culture medium containing different concentrations of the test samples. After 24 hours, use the MTT method to detect OD49omm, and use the t-test to analyze the effect of the test samples on the activity of RAW264.7 cells. (RAW264.7 is a mouse mononuclear macrophage cell line with typical macrophage functional characteristics, which can simulate the key immune process in skin inflammatory response).

[0095] This is embodied through the above technical solution.

[0096] Determination of TNF-a inflammatory factor content: RAW264.7 cells were plated in 96-well plates and cultured for 24 hours. The culture medium was replaced with basal culture medium containing different concentrations of test samples. After 24 hours, the cell supernatant was collected and centrifuged. The supernatant was aspirated and the TNF-a content in the cell culture supernatant was determined using an ELISA kit to obtain the effect of the test samples on the TNF-α content of macrophages.

[0097] (3) Calculation formula

[0098] TNF-α relative content (%) = (TNF-α content in sample / TNF-α content in negative control) * 100%

[0099] Cell tests were performed to verify the anti-inflammatory effect of the composition.

[0100] Before testing the TNF-a inflammatory factor content in the sample cells, cell activity screening is first performed to obtain the optimal test concentration.

[0101] (4) Test of TNF-a inflammatory factor content in cells

[0102] After screening the concentrations by cell activity, the best tested concentration of 0.0032% was selected to measure the TNF-a inflammatory factor content in RAW264.7 cells, and the effect of the sample on the TNF-a inflammatory factor content in RAW264.7 cells was obtained.

[0103] According to the laboratory method (HMC-WI-052 in vitro TNF-a inflammatory factor content determination), the overall cell activity and cellular TNF-α inflammatory factor content were tested at a test sample concentration of 0.0032%.

[0104] The test results are shown in Table 2.

[0105] Table 2

[0106] Experimental Grouping Cell viability Relative content of TNF-α (%) Example 1 better 47％ Example 2 better 53％ Example 3 better 52％ Comparative Example 1 better 87％ Comparative Example 2 better 85％ Comparative Example 3 better 98％ Comparative Example 4 Difference /

[0107] It can be seen from the data in the above table that 0.0032% of the test sample has a significant effect on inhibiting the content of TNF-a inflammatory factors in cells (statistical P<0.0001), that is, when the test sample is at a concentration of 0.0032%, the example has the ability to significantly inhibit the content of TNF-a inflammatory factors in cells, and the corresponding comparative example is not as capable of inhibiting the content of TNF-a inflammatory factors in cells as Examples 1-3.

[0108] Among them, the cell activity of Comparative Example 4 was poor, and the toxicological test using maltobionic acid alone showed that the cytotoxicity risk of maltobionic acid was controllable, indicating that the lower pH of Comparative Example 4 under high concentration of maltobionic acid had a great impact on cell activity, because low Ph may destroy cell membrane stability, interfere with enzyme activity and cell metabolism, leading to cell death or abnormal function, indicating that the content of TNF-a inflammatory factor in Comparative Example 4 may have increased.

[0109] Experimental Example 2 Application of Products

[0110] The compositions provided in the aforementioned embodiments and comparative examples were used to prepare cosmetics. The composition and proportion of the cosmetics are shown in Table 3.

[0111] Table 3 (wt%)

[0112]

[0113]

[0114] The performance of each of the above cosmetic samples was tested:

[0115] 1. Skin occlusive patch test

[0116] Test samples: samples of Examples 1 to 3 and Comparative Examples 1 to 4, a total of 7 samples, 14 volunteers with oily acne-sensitive skin, each of whom tested a different sample. Test site: normal skin on the flexor side of the forearm.

[0117] The specific steps are: clean the skin of the test area, take 0.025g of sample, place it in the small chamber of the spot tester (each tester has 10 small chambers, that is, each person tests 5 points), stick the skin spot test paper from bottom to top, stick it flat, and press it lightly with your hand a few times to expel the air.

[0118] Patch test time: 24 hours.

[0119] Observation time: 24 hours after application, remove the skin spot test consumables and observe the results after at least 30 minutes.

[0120] Result judgment:

[0121] Phenomenon 1: (+-)-Suspicious reaction: only faint (unclear) erythema.

[0122] Phenomenon 2: (+) - weak (non-blistering) positive reaction: erythema, infiltration, and possible small red rash.

[0123] Phenomenon 3: (++)--Strong (vesicular) positive reaction: erythema, infiltration, papules but not blisters.

[0124] Phenomenon 4: (+++) - Extreme positive reaction: redness, swelling and blisters.

[0125] Phenomenon 5: (-)--No reaction.

[0126] The results are shown in Table 4.

[0127] Table 4

[0128]

[0129]

[0130] Positive rate (%) = total number of occurrences (1-5) / total number of people * 100%

[0131] The test results in Table 4 show that among the subjects of Examples 1 to 3 and Comparative Examples 1 to 4, Comparative Examples 2, 3, and 4 showed suspicious reactions, and the rest showed no suspicious reactions or positive reactions. According to the "Technical Specifications for Safety of Cosmetics" (2015 edition), the test substance will not cause adverse skin reactions to the subjects of this batch. This shows that the formula of the acne-blocking composition provided in the embodiments of the present application is mild and safe, does not irritate the skin, and is suitable for sensitive oily acne skin.

[0132] 2. Sensitive oily skin a*- short-term effect test on acne removal, smoothing, pores, brightening and cheek redness

[0133] The efficacy test on sensitive and oily acne-prone skin is conducted through human trials, with a specific experimental population forming the test group. The changes in the skin surface of the subjects before and after using the samples on their faces are tested through polarized light sources, so as to evaluate the sample's a*-acne removal, smoothing, pores, brightening and cheek redness area efficacy.

[0134] There were 10 subjects in total, with obvious oily acne on their skin. The test period was 3 days, and the subjects used the samples on their faces once a day during the test period. Each subject used one sample on the left and right face for the test.

[0135] Experimental instrument: Skin imaging system

[0136] (1) The above experimental equipment was used to obtain the pictures. See the attached manual. Figure 1-Figure 4 .

[0137] in, Figure 1 , Figure 3This is the skin condition of the test subject before using the sample of Example 2. It can be seen that: obvious acne has grown, the skin has severe erythema and the acne scar area is relatively large; Figure 2 , Figure 4 This is the skin condition of the test subjects after using the sample of Example 2 for 3 days. It can be seen that: the acne area is significantly reduced, the skin erythema is significantly improved, the acne mark area is also greatly reduced, and there is no allergic reaction, indicating that the cosmetics using the acne-blocking composition of the present application can effectively improve the problems of acne, erythema and acne marks of oily and acne-sensitive skin, and have little irritation.

[0138] (2) The data of the skin test instrument is exported and the instrument test analysis data is as follows:

[0139] Table 5

[0140]

[0141] From the experimental results in the above table, we can see that:

[0142] 1. a*-acne removal, the red area of ​​acne was reduced by 12.15%, indicating that it effectively improved the redness and swelling of acne; 2. The skin smoothness of the acne area increased by 25%, indicating that it has a good smooth and closed effect. 3. The problem of enlarged pores on the facial skin was reduced by 17.24%, indicating that it has a fine pore effect. 4. The brightness of the facial skin increased by 5.32%, indicating that it has a good effect of improving red and black acne marks. 5. The area of ​​the red area on the cheeks was reduced by 6.26%, indicating that it has a good effect of improving erythema, blocking the occurrence of inflammation, and reducing the possibility of marks left by skin erythema.

[0143] This fully demonstrates that the composition of the present application can achieve the effect of removing acne and acne marks from different angles and aspects.

[0144] 3. Comparison with competitive products

[0145] We selected a commercially available product that is well-known on the market for its good acne scar lightening effect, a certain essence from OSM (a product with strong blemish lightening and scar removal effect, and collected market reviews on its effect of lightening acne and acne scars) for comparison, and selected 6 testers with oily, acne-sensitive skin to test its effects on acne and acne scar lightening for 7 days. Through VISA imaging, we tested and analyzed the ITA° value and a* value, and took the average value to calculate, which corresponded to the improvement of acne scars and the reduction of redness of acne, respectively.

[0146] The efficacy test for sensitive oily acne skin is conducted by human trials, with a specific experimental population as the test group. The changes in the skin surface of the subjects before and after using the sample are tested by polarized light sources, and the test values, ITA° value, L* value, a* value, are tested to evaluate the improvement of the sample in fading acne marks, acne marks, and a*-anti-acne cheek red areas. The test results are shown in Table 6 and the attached manual Figure 5-Figure 7 :

[0147] Table 6

[0148]

[0149]

[0150] From the above Table 6 and the Appendix Figure 5-Figure 7 It can be seen that:

[0151] 1. ITA° feedback is that the brightness of the corresponding skin on the face of the acne scars has increased by 3.00%, indicating that it has a good effect in improving the color of the acne scar area. 2. The L* value feedback is to evaluate the change in skin lightness and darkness, that is, the improvement effect of acne scars. The corresponding skin lightness and darkness has increased by 1.00%, indicating that compared with competing products, it has a good effect in improving red and black acne scars. 3. The a* value feedback is the area of ​​the corresponding skin cheek red area, that is, the improvement of the acne area, which has decreased by 3.04%, indicating that compared with competing products, it has greatly improved the situation of the cheek red area.

[0152] Comprehensive experimental data show that compared with competing products, this product has a better effect in improving erythema, blocking the occurrence of inflammation, and reducing the marks left by skin erythema.

[0153] 4. Sensory evaluation test

[0154] The samples prepared in Example 2 were tested, and 10 professional testers were selected to conduct a blind sensory evaluation test. The testers used it for 7 consecutive days, and the test items included fast absorption and non-stickiness, skin hydration, fading skin marks, improving enlarged skin pores, improving skin erythema, product irritation and overall evaluation. The scores of each test data were statistically analyzed, with a total score of 10 points. The higher the score, the higher the degree of recognition of the product by the subject.

[0155] The scoring results are shown in Table 7 below.

[0156] Table 7

[0157]

[0158]

[0159] From the above test results, it can be seen that the application of the acne-blocking composition of the present application has been highly recognized by the testers in terms of sensory perception. The data of the test items in the above table are averaged, and the test data of the aforementioned commercially available acne-blocking product (a certain essence of OSM) is taken from the historical database, and a radar chart is drawn for comparison. The results are as follows: Figure 8 As shown. Figure 8 It can be seen that the acne-removing composition of the present application that has the function of preventing acne scars has a higher sensory recognition than existing products.

[0160] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. An anti-acne composition for preventing acne marks, characterized in that: The acne-removing composition for preventing acne scars comprises, by weight: Maltobionic acid 0.5-2.5, arginine 0.2-1.5, peony root extract 1-10.

2. The anti-acne composition for preventing acne scars according to claim 1, characterized in that: The acne-removing composition for preventing acne scars comprises, by weight: Maltobionic acid 1.0-2.0, arginine 0.40-1.0, peony root extract 5-8.

3. The anti-acne composition for preventing acne scars according to claim 1, characterized in that: In the acne-removing composition for preventing acne scars, the mass ratio of the maltobionic acid to the arginine is (2-5):

1.

4. The anti-acne composition for preventing acne scars according to claim 1, characterized in that: In the acne-removing composition for preventing acne scars, the mass ratio of the maltobionic acid to the arginine is (2.5-4):

1.

5. The anti-acne composition for preventing acne scars according to any one of claims 1 to 4, characterized in that: The pH value of the acne-removing composition for preventing acne marks is 3.0-5.5; optionally, the pH value of the acne-removing composition for preventing acne marks is 4.0-5.

5.

6. The anti-acne composition for preventing acne scars according to any one of claims 1 to 4, characterized in that: The optical rotation of the arginine is in the range of 26.0° to 27.9°.

7. The anti-acne composition for preventing acne scars according to any one of claims 1 to 4, characterized in that: The peony root extract is added to the composition in the form of a solution; Optionally, in the peony root extract solution, the peony root extract is ≥8% by mass percentage; Optionally, in the peony root extract, the effective substance of paeoniflorin is ≥1% by mass percentage; Optionally, the peony root extract solution comprises peony root extract and a solvent; optionally, the solvent comprises butylene glycol, water, and 1,2-hexanediol.

8. Use of the acne-blocking and anti-acne composition according to any one of claims 1 to 7 in the preparation of cosmetics that inhibit TNF-a inflammatory factors in cells.

9. The use according to claim 8, characterized in that: In terms of volume percentage, the volume concentration of the acne-blocking acne-blocking composition in the aqueous solution of the acne-blocking composition is 0.0032%-2%.

10. A cosmetic, characterized in that: The cosmetic comprises the anti-acne composition for preventing acne marks according to any one of claims 1 to 7; Optionally, in the cosmetic, the amount of maltobionic acid added is 0.5%-2.5% by mass percentage; Optionally, in the cosmetic, the amount of arginine added is 0.2%-1.5% by mass; Optionally, in the cosmetic, the amount of the peony root extract added is 1%-10% by mass.

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