Traditional Chinese medicine composition with effect of improving skin aging and application of traditional Chinese medicine composition

Abstract

The invention discloses a traditional Chinese medicine composition with an effect of improving skin aging. The traditional Chinese medicine composition is prepared from saponin, lysimachia capillipes hemsl, herba lysimachiae and root of carmine. The traditional Chinese medicine composition has good effects of inhibiting skin inflammatory aging and improving skin elasticity, and can be applied to various skin care products such as masks, face cream and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a Chinese medicine composition having the effect of improving skin aging and a use thereof. Background Art

[0002] As human life span continues to increase, staying healthy during aging has become a new challenge for people. The skin degenerates during aging and becomes more fragile. There is a type of aging associated with chronic low-grade inflammation, called inflammatory aging.

[0003] Under normal circumstances, the human body has a certain amount of inflammation, which is physiological inflammation to maintain the balance of the internal environment. It is regulated by the protective ability of the skin barrier to prevent skin damage. However, with age and various external stimuli, the balance between skin regulation and destruction is broken, and the damage gradually becomes greater than the regulation, which will cause this physiological inflammation to be amplified. The damaged cells begin to release a large number of inflammatory factors. These inflammatory factors make the overall skin environment begin to become inflamed, further causing the extracellular matrix that maintains the elasticity and appearance of the skin to change from an orderly arrangement to a disordered arrangement. This situation will further amplify the inflammatory state, forming a vicious cycle that intersects with each other, and ultimately forming inflammatory aging. Therefore, inhibiting excessive inflammatory reactions is of great significance in alleviating skin aging.

[0004] Skin aging will also show increased wrinkles and decreased elasticity. The aging of the skin is closely related to the reduction of elastin. As the skin ages, the elastin in the skin cells will decrease, and the elasticity of the skin will also decrease. Photoaging mainly refers to aging caused by ultraviolet radiation. A large amount of ultraviolet radiation will activate the expression and activity of matrix metalloproteinases. Matrix metalloproteinases can degrade almost all molecules in the extracellular matrix. Among them, elastase can not only degrade elastin in the skin, but also activate other matrix metalloproteinases, accelerating the degradation of extracellular matrices such as collagen and elastin. In addition, long-term ultraviolet radiation will also produce a large amount of reactive oxygen, causing lipid peroxidation, resulting in fibroblast DNA damage and cell apoptosis, thereby leading to a decrease in elastin synthesis, which is ultimately manifested as decreased skin elasticity and wrinkle formation. Therefore, inhibiting the activity of elastase is also of great significance for alleviating skin aging.

[0005] Gleditsia sinensis Lam. is the fruit of the leguminous plant Gleditsia sinensis Lam. after aging or being damaged. Traditional Chinese medicine believes that it has the functions of clearing the orifices, removing phlegm, removing wind, and killing insects. It can be used to treat stroke, lockjaw, wind, wind chronicity, throat paralysis, phlegm and asthma, fullness and stagnation, obstruction of the body, carbuncle, scabies, ringworm, head sore and other diseases. Gleditsia is also a commonly used cleaning material. For example, it was used to wash clothes in ancient times; in modern times, many shampoos and other daily chemical products have added gleditsia.

[0006] Herba Lysimachiae capillipes Hemsl. is the whole herb of the Primulaceae plant Lysimachia capillipes Hemsl. It has the effects of dispelling wind and dampness, promoting qi and relieving pain, regulating menstruation, and detoxifying. It is used for colds, coughs, rheumatic pain, abdominal distension and pain, irregular menstruation, furuncles, and snake bites.

[0007] The medicine for furuncle is the root, stem and leaf of Passiflora cupiformis Passif, a plant of the Passifloraceae family. It has the effects of dispelling wind and dampness, promoting blood circulation and relieving pain, nourishing the heart and calming the mind. It is commonly used for rheumatic heart disease, hematuria, leukorrhea, hemiplegia, furuncle, traumatic bleeding, and abdominal distension and pain caused by shaqi.

[0008] The root of carmine flower is the tuberous root of Mirabilis jalapa L., a plant of the Mirabilis jalapa family. The root of carmine flower is sweet and light in taste, cool in nature, and enters the lung meridian. It has the effects of promoting blood circulation and regulating menstruation, clearing away heat and dampness, dispersing blood stasis and reducing swelling.

[0009] The inventors found in the previous research process that a specific composition of Gleditsia sinensis, Herba Lycopodii, Herba Lycopodii and Herba Corydalis has good anti-inflammatory and elastase inhibition activity. Based on the above reasons, the present invention provides a Chinese medicine composition with the effect of improving skin aging, which is composed of Gleditsia sinensis, Herba Lycopodii, Herba Lycopodii and Herba Corydalis. The Chinese medicine composition has good anti-inflammatory and elastase inhibition activity and can be used in various skin care products such as facial masks and creams. Summary of the invention

[0010] The main purpose of the present invention is to provide a traditional Chinese medicine composition with definite efficacy, few side effects, and natural safety for improving skin aging, so as to solve the problems of poor anti-skin aging effect or easy allergy of common skin care products in the prior art.

[0011] In order to achieve the above object, according to one aspect of the present invention, a Chinese medicine composition having the effect of improving skin aging is provided, characterized in that the Chinese medicine composition is composed of 5 parts of Gleditsia sinensis, 15 parts of Herba Lycopodii, 25 parts of Herba Carmichaelii and 45 parts of Herba Corydalis.

[0012] Furthermore, the traditional Chinese medicine composition further comprises one or more drugs and / or extracts for anti-inflammatory and antioxidant effects.

[0013] Furthermore, the anti-inflammatory drug is aspirin, salicylic acid, acetaminophen, or amoxicillin.

[0014] Furthermore, the antioxidant drug is vitamin C, vitamin E, resveratrol, quercetin, and dihydroquercetin.

[0015] According to another aspect of the present invention, there is provided a use of the above-mentioned Chinese medicine composition in improving skin aging and its application in various skin care products such as facial masks and facial creams.

[0016] Beneficial effects of the present invention:

[0017] The technical scheme of the present invention is applied, and the Chinese medicine composition of the present invention is composed of 5 parts of Gleditsia sinensis, 15 parts of Herba Lycopodii, 25 parts of Herba Lycopodii and 45 parts of Herba Corydalis. The Chinese medicine composition has good anti-inflammatory and elastase inhibition activities, can significantly improve the level of cellular inflammatory factors, and well solves the problems of poor effect and easy allergic reaction of common anti-skin aging skin care products. DETAILED DESCRIPTION

[0018] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0019] The present invention is intended to encompass all alternatives, variations and equivalents that may be included in the present invention as defined by the claims. Those skilled in the art will recognize many methods and materials similar or equivalent to those described herein that can be used in the practice of the present invention. The present invention is in no way limited to the methods and materials described.

[0020] Unless otherwise specified or there is a clear conflict in context, the articles "a", "an", and "the" used in this invention are intended to include "at least one" or "one or more". Therefore, these articles used in this invention refer to one or more than one (i.e., at least one) object.

[0021] As described in the background technology section, existing common anti-aging skin care products have poor effects and are prone to allergic reactions. In order to solve the above problems, the present invention provides a Chinese medicine composition, which is 5 parts of Gleditsia sinensis, 15 parts of Herba Lycopodii, 25 parts of Herba Lycopodii and 45 parts of Herba Corydalis.

[0022] In this embodiment, the traditional Chinese medicine composition further comprises one or more drugs and / or extracts for anti-inflammatory and antioxidant effects.

[0023] Furthermore, the anti-inflammatory drug is aspirin, salicylic acid, acetaminophen, or amoxicillin.

[0024] Furthermore, the antioxidant drug is vitamin C, vitamin E, resveratrol, quercetin, and dihydroquercetin.

[0025] The present invention also provides a use of the Chinese medicine composition in improving skin aging and its application in various skin care products such as facial masks and facial creams.

[0026] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present invention will be described in detail below in conjunction with the embodiments.

[0027] The present invention is further described in detail below in conjunction with specific embodiments. These embodiments should not be construed as limiting the scope of protection claimed in this application.

[0028] Example

[0029] Example 1 Preparation of Chinese medicine composition 1-11

[0030] The Chinese medicine composition 1-11 of the present invention adopts a uniform design experimental scheme: referring to the literature (Liang Maoxin, Li Dongan. Improved application of uniform design in the selection of small Chinese medicine compound prescriptions [J]. World Science and Technology - Modernization of Traditional Chinese Medicine, 2006, 8 (02): 53-56.), the uniform design scheme U11 (11 4 )(As shown in Table 1), four Chinese medicinal materials, namely, sapodilla, fragrant herb, forked carbuncle medicine and rhizome of carmine, were used as investigation factors in the prescription. Each factor had 11 levels (i.e., 11 different doses with increasing gradient). The investigation dose was between 0 and 50 g. Factors and levels were arranged according to the uniform design table, and prescription design was carried out to construct a total of 11 groups of Chinese medicine compositions.

[0031] Table 1 Chinese medicine composition U11(11 4 )Uniform Design Table

[0032]

[0033]

[0034] Preparation of Chinese medicine composition 1-11 According to the amount of medicinal materials of composition 1-11 in Table 1, weigh each medicinal material according to the prescription of each group, crush it into coarse powder, and mix it evenly. According to the total amount of medicinal materials in each group, add 12 times the amount of purified water, soak for 1 hour, decoct for 1 hour, cool, filter, and collect the filtrate. Add 10 times the amount of purified water of the original medicinal material to the residue, decoct again for 1 hour, cool, filter, combine the filtrates of the two decoctions, and concentrate to 2 times the volume of the original medicinal material (volume / mass ratio), let it stand to 70°C, add 3 times the amount of 95% ethanol while hot, let it stand for more than 4 hours, take the supernatant, reduce pressure to recover ethanol and concentrate, and dry the concentrate at 60°C under reduced pressure to obtain the extract of Chinese medicine composition 1-11.

[0035] Example 2 Evaluation of the Cellular Anti-inflammatory Activity of Chinese Medicine Compositions 1-11

[0036] Experimental Drugs Test drug compositions 1 to 11 were prepared according to the method of Example 1.

[0037] Experimental method: Raw264.7 cells in the logarithmic growth phase were taken and prepared into a single cell suspension using complete medium containing 10% FBS and 1% streptomycin. The concentration was adjusted to 10 5 / mL, inoculated in a 96-well plate, cultured for 18 hours, and after the cells adhered to the wall, 0.1g / mL of the extracts of the Chinese medicine composition 1-11 were added, and after pretreatment for 5 hours, 1.0μg / mL of lipopolysaccharide (LPS) was added. After incubation for 24 hours, the 96-well plate was placed on a centrifuge and centrifuged at 1000×g for 10 minutes. The supernatant was taken and the NO content was determined according to the instructions of the nitric oxide kit (Shanghai ELISA Biotechnology Co., Ltd.). The supernatant was taken separately and the contents of inflammatory factors IL-6, IL-1β, and TNF-α were determined according to the instructions of the enzyme-linked immunosorbent assay kit (Shanghai ELISA Biotechnology Co., Ltd.). The results are shown in Table 2.

[0038] Table 2 Effects of different compositions on NO and inflammatory factors in RAW264.7 cells induced by lipopolysaccharide (n=5)

[0039]

[0040]

[0041] Note: Compared with the model group, *p<0.05;

[0042] Experimental results From the data in Table 2, it can be seen that Chinese medicine composition 2, Chinese medicine composition 3, Chinese medicine composition 4, and Chinese medicine composition 9 can effectively inhibit the release of cellular NO and inflammatory factors, and show good anti-inflammatory activity. In particular, Chinese medicine composition 2 (5 parts of Gleditsia sinensis, 15 parts of Herba Lycopodii, 25 parts of Herba Lycopodii, and 45 parts of Herba Corydalis) has the strongest anti-inflammatory activity.

[0043] Example 3 Inhibitory activity of Chinese medicine composition 1-11 on elastase

[0044] Experimental Drugs Test drug compositions 1 to 11 were prepared according to the method of Example 1.

[0045] Experimental method: 100 μL of each drug composition, elastase solution (0.5 U / mL) and Tris-HCl buffer (0.1 mol / L, pH = 8) were added to a 96-well plate, mixed by shaking, incubated at 37°C for 15 min, 50 μl SANA (1 mmol / L) was added, incubated at 37°C for 20 min, mixed by shaking, and the absorbance was measured at 410 nm. EGCG was used as a positive control, all samples were repeated three times, the average value was calculated, and the inhibition rate of elastase by different drug compositions was calculated according to the following formula.

[0046] Inhibition rate (%) = (1-A 空白 / A 样品 )×100%

[0047] Table 3 Inhibition rate of different drug compositions on elastase

[0048]

[0049] Note: Compared with the positive control group, *p<0.05;

[0050] The experimental results are shown in Table 3. Compared with the positive control group, composition 2 (5 parts of saponins, 15 parts of fragrant grass, 25 parts of Herba Lycopodii and 45 parts of Herba Caryophylli) has better elastase inhibitory activity, indicating that the medicinal composition of saponins, fragrant grass, Herba Caryophylli and Herba Caryophylli in a specific proportion has a synergistic inhibitory effect on elastase.

[0051] Example 4 Preparation of Chinese medicine composition 2 facial mask

[0052] Mask formula composition: water 84.48%, Chinese medicine composition 2 extract 9.0%, glyceryl polyoxyethylene G-18 2.0%, 1,4-butylene glycol 0.5%, glycerin 2.5%, polydimethylsiloxane 0.5%, sodium hyaluronate 0.25%, purslane extract 0.5%, EDTA 0.05%, 1,2-hexanediol 0.15%, caprylhydroxamic acid 0.05%, PEG-40 hydrogenated castor oil 0.02%.

[0053] Preparation process: Add the prescribed amount of water, extract of Chinese medicine composition 2, glycerin and polydimethylsiloxane into the main pot, stir and heat to 85°C to fully dissolve them; then add 1,4-butanediol, sodium hyaluronate and EDTA and continue to stir evenly; cool to 45°C and add the remaining components in turn, continue to stir slowly and evenly, and after passing the inspection, fill, package, inspect the finished products and put them into storage.

[0054] Preliminary volunteer trials showed that the facial mask prepared with the extract of Chinese medicine composition 2 had no bad odor or irritation, and had obvious anti-inflammatory and skin elasticity improvement effects.

[0055] The above is only an embodiment of the present invention, and the common knowledge such as the known specific structure and characteristics in the scheme is not described in detail here. It should be pointed out that for those skilled in the art, several modifications and improvements can be made without departing from the present invention, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

[0056] The embodiments of the present invention are described in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. At the same time, changes or deformations made by those skilled in the art based on the ideas of the present invention, the specific implementation methods and application scope of the present invention, all belong to the scope of protection of the present invention. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A Chinese medicine composition having the effect of improving skin aging, characterized in that: The traditional Chinese medicine composition is prepared from 5 parts of sapodilla, 15 parts of fragrant sedge, 25 parts of scutellaria baicalensis and 45 parts of rhizoma carmichaelii roots.

2. The Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is further used in combination with one or more anti-inflammatory and anti-oxidative drugs and / or Chinese medicine extracts.

3. The Chinese medicine composition according to claim 2, characterized in that: The anti-inflammatory drugs are aspirin, salicylic acid, acetaminophen and amoxicillin.

4. The Chinese medicine composition according to claim 2, characterized in that: The antioxidant drugs are vitamin C, vitamin E, resveratrol, quercetin and dihydroquercetin.

5. The skin aging improving effect of the Chinese medicine composition according to claims 1-4 and its application in various skin care products such as facial masks and facial creams.

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