Sand therapy mineral sand for improving skin inflammation as well as preparation method and application of sand therapy mineral sand

Abstract

The invention relates to the field of sand therapy supplies, in particular to sand therapy mineral sand for improving skin inflammation, a preparation method and application. The invention discloses a green tea mask which is composed of a matrix and a microcapsule, the matrix is composed of Yellow River sandy soil, volcanic mud and green tea powder, the microcapsule comprises an inner layer and an outer layer, the inner layer is composed of a maltodextrin-esterified witch hazel composite material, an aloe extract and vitamin E, and the outer layer is composed of Arabic gum-nano zeolite and hyaluronic acid. When the mineral sand contacts with inflammation skin, physical stimulation of the Yellow River sandy soil promotes blood circulation, the volcanic mud supplements nutrition, and the green tea powder plays roles in resisting oxidation and diminishing inflammation. Maltodextrin in the inner layer of the microcapsule wraps vitamin E, esterifies witch hazel to enhance stability, and forms a pocket structure with aloe polysaccharide to fix active ingredients. The inner layer and the outer layer of the microcapsule are combined to construct a semi-permeable membrane, macromolecule diffusion is limited, and differentiated release is achieved. The nano zeolite enhances the structural strength, adsorbs inflammatory factors and reduces inflammatory response.

Description

Technical Field

[0001] The invention relates to the field of sand therapy products, and in particular to sand therapy mineral sand for improving skin inflammation, a preparation method and application thereof. Background Art

[0002] Skin inflammation is a common health problem in modern society. Its pathogenesis is complex and involves multiple factors such as environment, physiology and genetics. At present, sand therapy mineral sand for improving skin inflammation has shown good therapeutic effects in clinical applications and has become an important physical therapy method. Traditional sand therapy mineral sand is mainly composed of natural mineral sand and other materials, and improves skin inflammation through its unique physical properties and mineral components.

[0003] However, as people's requirements for therapeutic effects increase, traditional sand therapy mineral sands have shown obvious limitations in the application of active ingredients. Currently, the sand therapy mineral sands on the market that improve skin inflammation usually use a simple mixing method to add various active ingredients, such as vitamins, plant extracts, etc. Although this approach can have a certain therapeutic effect in the short term, there are serious problems with the stability of the active ingredients.

[0004] First, most active ingredients are exposed to the external environment and are easily degraded by factors such as temperature, light, and oxidation, which greatly reduces their effectiveness. Second, some active ingredients may interact with each other, affecting their respective efficacy. Third, due to the lack of effective protection measures, these active ingredients are easily lost during storage and use, resulting in a short shelf life of the product and insufficient therapeutic effect. In addition, the active ingredients are not tightly bound to the matrix and are easily separated during use, affecting the uniformity and stability of the product.

[0005] These problems seriously affect the therapeutic effect and usage experience of sand therapy mineral sand, and also limit its further development in the field of skin inflammation treatment. Summary of the invention

[0006] (1) Technical issues to be solved

[0007] The purpose of the present invention is to provide a sand therapy mineral sand for improving skin inflammation and a preparation method thereof, so as to effectively relieve skin inflammation and solve the stability problem of active ingredients.

[0008] (2) Technical solution

[0009] To achieve the above objectives, on the one hand, the present invention provides a sand therapy mineral sand for improving skin inflammation, which is composed of a matrix and microcapsules, wherein the matrix is ​​Yellow River sand, volcanic mud and green tea powder, and the microcapsules include an inner layer and an outer layer, the inner layer is maltodextrin-esterified witch hazel composite material, aloe extract and vitamin E, and the outer layer is gum arabic-nano zeolite and hyaluronic acid.

[0010] Furthermore, the matrix includes the following raw materials in parts by weight: 70-80 parts of Yellow River sand, 15-20 parts of volcanic mud, and 3-5 parts of green tea powder; the inner layer of the microcapsule includes the following raw materials in parts by weight: 8-12 parts of maltodextrin-esterified witch hazel composite material, 3-5 parts of aloe extract, and 1-2 parts of vitamin E; the outer layer of the microcapsule includes the following raw materials in parts by weight: 8-10 parts of gum arabic-nano zeolite and 2-3 parts of hyaluronic acid; wherein the Yellow River sand comes from the Loess Plateau, mainly the loess hilly gully area in the middle reaches of the Yellow River.

[0011] Furthermore, the preparation method of the maltodextrin-esterified witch hazel composite material comprises:

[0012] S11. Place the witch hazel extract in a three-necked flask, then add citric acid and anhydrous ethanol to obtain a mixture A, reflux the mixture A in a water bath at 70 to 80° C. for 4 to 5 hours to obtain a mixture B;

[0013] S12. The mixture B is filtered to obtain a mixture C, the mixture C is washed with anhydrous ethanol for 3 to 4 times to obtain a washing D, and the washing D is vacuum dried at 60 to 70° C. for 12 to 20 hours to obtain esterified witch hazel;

[0014] S13. adding maltodextrin to deionized water to obtain a maltodextrin solution, adjusting the pH of the maltodextrin solution to 5.5 to 6.0 to obtain a maltodextrin solution after adjusting the pH, and heating the maltodextrin solution after adjusting the pH to 60 to 70° C. to obtain a heated maltodextrin solution;

[0015] S14. Adding the esterified witch hazel to the heated maltodextrin solution to obtain a first mixed solution, stirring the first mixed solution with a high-speed disperser at 3000-4000 r / min for 30-40 min to obtain a second mixed solution, stirring the second mixed solution at 60-70° C. for 2-3 h to obtain a third mixed solution, and cooling the third mixed solution to room temperature to obtain a fourth mixed solution;

[0016] S15. freeze-dry the fourth mixed solution for 12 to 24 hours to obtain a maltodextrin-esterified witch hazel composite material.

[0017] Furthermore, the preparation method of the gum arabic-nano zeolite comprises:

[0018] S21. Add nano zeolite to deionized water, then ultrasonicate for 30 to 40 minutes to obtain a nano zeolite dispersion, add sodium chloride solution to the nano zeolite dispersion to obtain a mixed solution, ultrasonicate the mixed solution for 15 to 20 minutes to obtain a mixed solution after ultrasonication, centrifuge the mixed solution after ultrasonication at 8000 to 9000 r / min for 10 to 15 minutes to obtain a centrifuged mixture, wash the centrifuged mixture with anhydrous ethanol for 3 to 4 times to obtain a washed mixture, and vacuum dry the washed mixture at 60 to 70° C. for 12 to 24 hours to obtain a pretreated nano zeolite;

[0019] S22. Adding gum arabic to deionized water, stirring for 2 to 3 hours to obtain a gum arabic solution, adjusting the pH of the gum arabic solution to 6.5 to 7.0 to obtain a pH-adjusted gum arabic solution, and filtering the pH-adjusted gum arabic solution to obtain a filtrate;

[0020] S23. Slowly add the pretreated nano zeolite to the filtrate, then ultrasonicate for 30 to 40 minutes to obtain solution A, stir solution A at 50 to 60° C. and 500 to 600 r / min for 2 to 3 hours to obtain solution B, adjust the pH of solution B to 7.0 to 7.5 to obtain solution C, stir solution C for 1 to 2 hours to obtain solution D, centrifuge solution D at 6000 to 7000 r / min for 15 to 20 minutes to obtain a supernatant;

[0021] S24. Freeze-dry the supernatant for 24 to 48 hours to obtain gum arabic-nano zeolite.

[0022] On the other hand, based on the same inventive concept, the present invention also provides a method for preparing sand therapy mineral sand for improving skin inflammation, which is applied to the sand therapy mineral sand for improving skin inflammation, and comprises the following steps:

[0023] S31. Passing the Yellow River sand through a 100-120 mesh sieve to obtain sieved Yellow River sand, drying the sieved Yellow River sand at 100-120° C. for 4-5 hours to obtain dried Yellow River sand, and cooling the dried Yellow River sand to room temperature to obtain pretreated Yellow River sand;

[0024] S32. crushing the volcanic mud through a 200-220 mesh sieve to obtain sieved volcanic mud, drying the sieved volcanic mud at 105-110° C. for 2-3 hours to obtain dried volcanic mud, and cooling the dried volcanic mud to room temperature to obtain pretreated volcanic mud;

[0025] S33. The green tea powder is sieved through a 200-220 mesh sieve to obtain pretreated green tea powder;

[0026] S34. dissolving the maltodextrin-esterified witch hazel composite material in deionized water, then adding aloe vera extract to obtain solution A, stirring solution A at 500-600 r / min for 30-40 min to obtain stirred solution A, adding vitamin E to the stirred solution A to obtain solution B, ultrasonicating solution B for 15-30 min to obtain ultrasonicated solution B, adjusting the pH of ultrasonicated solution B to 6.5-7.0 to obtain an inner core material solution;

[0027] S35. The gum arabic-nano zeolite is dispersed in deionized water and ultrasonically treated for 20 to 30 minutes to obtain a gum arabic-nano zeolite dispersion, a hyaluronic acid solution is added to the gum arabic-nano zeolite dispersion to obtain a solution C, the pH of the solution C is adjusted to 7.0 to 7.5 to obtain a solution D, and the solution D is stirred at 400 to 500 r / min for 30 minutes to obtain an outer coating material;

[0028] S36. The inner core material solution is added dropwise to the outer coating material at a rate of 2 to 3 mL / min, and stirred at 800 to 900 r / min for 2 to 3 h to obtain a solution E, and the solution E is spray-dried at an inlet temperature of 155 to 165° C., an outlet temperature of 75 to 85° C., an atomization pressure of 0.2 to 0.3 MPa, and a feed rate of 15 to 18 mL / min to obtain microcapsules;

[0029] S37. The pretreated Yellow River sand and the pretreated volcanic mud are mixed and stirred at high speed for 5 to 10 minutes to obtain a first mixture, the pretreated green tea powder is added to the first mixture to obtain a second mixture, and the second mixture is further mixed for 3 to 5 minutes to obtain a mixed matrix;

[0030] S38. Sprinkle the microcapsules evenly on the surface of the mixed matrix, and stir and mix at a low speed for 10 to 15 minutes to obtain a third mixture, pass the third mixture through an 80 to 90 mesh sieve to obtain a sieved third mixture, and vacuum dry the sieved third mixture at 50 to 60° C. for 2 to 3 hours to obtain sand therapy mineral sand for improving skin inflammation.

[0031] The mechanism of action of the above raw material components is as follows:

[0032] Yellow River Sand: Through its unique mineral composition and appropriate particle size distribution, it provides mild physical stimulation on the skin surface, thereby promoting local blood circulation and creating favorable conditions for subsequent treatment.

[0033] Volcanic mud: rich in various trace elements and minerals, it can penetrate into the surface of the skin, provide nutritional supplements to the skin, and also improve the skin microenvironment.

[0034] Green tea powder: The active substances such as catechins and tea polyphenols have dual antioxidant and anti-inflammatory effects. They begin to work in the early stage of contact with the skin and create a good microenvironment for subsequent treatment.

[0035] Maltodextrin-esterified witch hazel composite material: Maltodextrin forms a cage-type structure with a hydrophobic inner cavity through its ring structure to encapsulate fat-soluble active substances, while esterified witch hazel forms a hydrogen bond network with maltodextrin through its unique ester group and hydroxyl group, thereby enhancing the mechanical strength and stability of the inclusion system.

[0036] Aloe vera extract: The polysaccharides in it can form hydrophobic interactions with the ester groups in the witch hazel molecules, increasing the volume and density of the hydrophobic region, forming a stable "pocket" structure, and improving the encapsulation efficiency and stability of the active ingredients.

[0037] Vitamin E: As a key fat-soluble active substance, it is stably encapsulated by the cage structure of maltodextrin, thereby preventing it from oxidative degradation and maintaining its activity.

[0038] Gum Arabic-Nano Zeolite: The carboxyl groups on the gum Arabic molecular chain form covalent bonds with the silanol groups on the surface of the nano zeolite, while forming a hydrogen bond network with the inner layer material to construct a semipermeable membrane with a network structure, achieving differentiated release of different components and adsorbing inflammatory factors through the ion exchange sites of the nano zeolite.

[0039] Hyaluronic acid: As a water-soluble active ingredient, it is stored in the three-dimensional pore structure of nano zeolite, which achieves a larger storage capacity and better release effect.

[0040] (3) Beneficial effects

[0041] Compared with the prior art, the present invention has the following beneficial effects:

[0042] 1. Through the designed microcapsule structure, a stable multilayer system was constructed using maltodextrin, esterified witch hazel and gum arabic, which improved the stability and encapsulation efficiency of the active ingredients.

[0043] 2. The abundant ion exchange sites on the surface of nano-zeolite can adsorb inflammatory factors on the skin surface through electrostatic action, thereby reducing local inflammatory responses. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 This is a physical picture of the sand therapy mineral sand according to Example 1 of the present invention. DETAILED DESCRIPTION

[0045] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0046] The test equipment and preparations of the examples described below are as follows:

[0047] Electronic balance (Sartorius, Germany), electric blast constant temperature dryer (Shanghai Fomar Experimental Equipment), electric constant temperature water bath (Jiangsu Kedao), grinder (Shandong Tianfang Machinery), magnetic stirrer (Shanghai Meiyingpu), pH meter (Qingdao Mingcheng), centrifuge (Hunan Kaida), vacuum drying oven (Shanghai Jiecheng), ultrasonic cleaner (Shanghai Bilang), high-speed disperser (Kunshan Desi), spray dryer (Shanghai Bilang); chemicals and reagents were purchased from Sigma-Aldrich.

[0048] Example 1: This example discloses a sand therapy mineral sand for improving skin inflammation, which is composed of a matrix and microcapsules. The matrix is ​​Yellow River sand, volcanic mud and green tea powder. The microcapsules include an inner layer and an outer layer. The inner layer is maltodextrin-esterified witch hazel composite material, aloe extract and vitamin E, and the outer layer is gum arabic-nano zeolite and hyaluronic acid.

[0049] The matrix comprises the following raw materials in parts by weight: 72 parts of Yellow River sand, 16 parts of volcanic mud, and 3 parts of green tea powder; the inner layer of the microcapsule comprises the following raw materials in parts by weight: 11 parts of maltodextrin-esterified witch hazel composite material, 3 parts of aloe extract, and 1 part of vitamin E; the outer layer of the microcapsule comprises the following raw materials in parts by weight: 10 parts of gum arabic-nano zeolite and 2 parts of hyaluronic acid.

[0050] When mineral sand comes into contact with inflamed skin, such as Figure 1As shown in the figure, the matrix layer plays a role first. The Yellow River sand has a unique mineral composition and suitable particle size distribution, which can provide mild physical stimulation and promote local blood circulation. The trace elements and minerals rich in volcanic mud can penetrate into the surface of the skin to supplement nutrition. At the same time, the active substances such as catechins and tea polyphenols in green tea powder begin to play a preliminary antioxidant and anti-inflammatory role, creating a good microenvironment for subsequent treatment. Subsequently, the microcapsules begin to slowly release active ingredients. The maltodextrin molecules in the inner layer form a cage structure with a hydrophobic inner cavity through their ring structure. This structure can stably encapsulate fat-soluble active substances such as vitamin E to prevent their oxidative degradation. The esterified witch hazel molecules form a hydrogen bond network with the maltodextrin molecules through their unique ester groups and hydroxyl groups, which can enhance the mechanical strength and stability protection of the inclusion system. At the same time, the ester groups in the witch hazel molecules can also form hydrophobic interactions with the polysaccharides in the aloe extract. This interaction can increase the volume and density of the hydrophobic region, increasing the space that can accommodate fat-soluble active ingredients. Secondly, this aggregation will make the molecules in the hydrophobic region more tightly arranged, forming a stable "pocket" structure. These "pockets" can more firmly fix the active ingredients to prevent them from leaking or degrading, thereby further improving the encapsulation efficiency of the active ingredients. The outer layer of gum arabic, as a natural polysaccharide, has abundant carboxyl groups on its molecular chain that can not only form covalent bonds with the silanol groups (Si-OH) on the surface of the nanozeolite, but also form a hydrogen bond network with the hydroxyl and ester groups on the surface of the inner layer of maltodextrin-esterified witch hazel composite material, significantly enhancing the binding stability between the inner and outer layers. In addition, the ester groups in the witch hazel molecule can also produce hydrophobic interactions with the hydrophobic regions of gum arabic, and some of the negatively charged groups in the esterified witch hazel can electrostatically attract the cationic sites on the surface of the nano-zeolite. These interactions together construct a semi-permeable membrane with a mesh structure. The pore size in the mesh structure can limit the rapid diffusion of large molecular active ingredients, while the hydrophobic regions and ion exchange sites in the network can achieve differentiated release of different ingredients. In addition, the introduction of nano-zeolites not only enhances the mechanical strength of the outer layer structure, but its unique three-dimensional pore structure also provides additional storage space, which can load more water-soluble active ingredients such as hyaluronic acid. More importantly, the rich ion exchange sites on the surface of nano-zeolites can adsorb inflammatory factors on the skin surface through electrostatic effects, thereby reducing local inflammatory reactions.

[0051] The preparation method of the maltodextrin-esterified witch hazel composite material comprises:

[0052] S11. Place the witch hazel extract in a three-necked flask, then add citric acid and anhydrous ethanol to obtain a mixture A, reflux the mixture A in a water bath at 70 to 80° C. for 4 to 5 hours to obtain a mixture B;

[0053] S12. The mixture B is filtered to obtain a mixture C, the mixture C is washed with anhydrous ethanol for 3 to 4 times to obtain a washing D, and the washing D is vacuum dried at 60 to 70° C. for 12 to 20 hours to obtain esterified witch hazel;

[0054] S13. adding maltodextrin to deionized water to obtain a maltodextrin solution, adjusting the pH of the maltodextrin solution to 5.5 to 6.0 to obtain a maltodextrin solution after adjusting the pH, and heating the maltodextrin solution after adjusting the pH to 60 to 70° C. to obtain a heated maltodextrin solution;

[0055] S14. Adding the esterified witch hazel to the heated maltodextrin solution to obtain a first mixed solution, stirring the first mixed solution with a high-speed disperser at 3000-4000 r / min for 30-40 min to obtain a second mixed solution, stirring the second mixed solution at 60-70° C. for 2-3 h to obtain a third mixed solution, and cooling the third mixed solution to room temperature to obtain a fourth mixed solution;

[0056] S15. freeze-dry the fourth mixed solution for 12 to 24 hours to obtain a maltodextrin-esterified witch hazel composite material.

[0057] The preparation method of the gum arabic-nano zeolite comprises:

[0058] S21. Add nano zeolite to deionized water, then ultrasonicate for 30 to 40 minutes to obtain a nano zeolite dispersion, add sodium chloride solution to the nano zeolite dispersion to obtain a mixed solution, ultrasonicate the mixed solution for 15 to 20 minutes to obtain a mixed solution after ultrasonication, centrifuge the mixed solution after ultrasonication at 8000 to 9000 r / min for 10 to 15 minutes to obtain a centrifuged mixture, wash the centrifuged mixture with anhydrous ethanol for 3 to 4 times to obtain a washed mixture, and vacuum dry the washed mixture at 60 to 70° C. for 12 to 24 hours to obtain a pretreated nano zeolite;

[0059] S22. Adding gum arabic to deionized water, stirring for 2 to 3 hours to obtain a gum arabic solution, adjusting the pH of the gum arabic solution to 6.5 to 7.0 to obtain a pH-adjusted gum arabic solution, and filtering the pH-adjusted gum arabic solution to obtain a filtrate;

[0060] S23. Slowly add the pretreated nano zeolite to the filtrate, then ultrasonicate for 30 to 40 minutes to obtain solution A, stir solution A at 50 to 60° C. and 500 to 600 r / min for 2 to 3 hours to obtain solution B, adjust the pH of solution B to 7.0 to 7.5 to obtain solution C, stir solution C for 1 to 2 hours to obtain solution D, centrifuge solution D at 6000 to 7000 r / min for 15 to 20 minutes to obtain a supernatant;

[0061] S24. Freeze-dry the supernatant for 24 to 48 hours to obtain gum arabic-nano zeolite.

[0062] A method for preparing sand therapy mineral sand for improving skin inflammation, which is applied to the sand therapy mineral sand for improving skin inflammation, comprises the following steps:

[0063] S31. Passing the Yellow River sand through a 100-120 mesh sieve to obtain sieved Yellow River sand, drying the sieved Yellow River sand at 100-120° C. for 4-5 hours to obtain dried Yellow River sand, and cooling the dried Yellow River sand to room temperature to obtain pretreated Yellow River sand;

[0064] S32. crushing the volcanic mud through a 200-220 mesh sieve to obtain sieved volcanic mud, drying the sieved volcanic mud at 105-110° C. for 2-3 hours to obtain dried volcanic mud, and cooling the dried volcanic mud to room temperature to obtain pretreated volcanic mud;

[0065] S33. The green tea powder is sieved through a 200-220 mesh sieve to obtain pretreated green tea powder;

[0066] S34. dissolving the maltodextrin-esterified witch hazel composite material in deionized water, then adding aloe vera extract to obtain solution A, stirring solution A at 500-600 r / min for 30-40 min to obtain stirred solution A, adding vitamin E to the stirred solution A to obtain solution B, ultrasonicating solution B for 15-30 min to obtain ultrasonicated solution B, adjusting the pH of ultrasonicated solution B to 6.5-7.0 to obtain an inner core material solution;

[0067] S35. The gum arabic-nano zeolite is dispersed in deionized water and ultrasonically treated for 20 to 30 minutes to obtain a gum arabic-nano zeolite dispersion, a hyaluronic acid solution is added to the gum arabic-nano zeolite dispersion to obtain a solution C, the pH of the solution C is adjusted to 7.0 to 7.5 to obtain a solution D, and the solution D is stirred at 400 to 500 r / min for 30 minutes to obtain an outer coating material;

[0068] S36. The inner core material solution is added dropwise to the outer coating material at a rate of 2 to 3 mL / min, and stirred at 800 to 900 r / min for 2 to 3 h to obtain a solution E, and the solution E is spray-dried at an inlet temperature of 155 to 165° C., an outlet temperature of 75 to 85° C., an atomization pressure of 0.2 to 0.3 MPa, and a feed rate of 15 to 18 mL / min to obtain microcapsules;

[0069] S37. The pretreated Yellow River sand and the pretreated volcanic mud are mixed and stirred at high speed for 5 to 10 minutes to obtain a first mixture, the pretreated green tea powder is added to the first mixture to obtain a second mixture, and the second mixture is further mixed for 3 to 5 minutes to obtain a mixed matrix;

[0070] S38. Sprinkle the microcapsules evenly on the surface of the mixed matrix, and stir and mix at a low speed for 10 to 15 minutes to obtain a third mixture, pass the third mixture through an 80 to 90 mesh sieve to obtain a sieved third mixture, and vacuum dry the sieved third mixture at 50 to 60° C. for 2 to 3 hours to obtain sand therapy mineral sand for improving skin inflammation.

[0071] Example 2: This example discloses a sand therapy mineral sand for improving skin inflammation, which is composed of a matrix and microcapsules. The matrix is ​​Yellow River sand, volcanic mud and green tea powder. The microcapsules include an inner layer and an outer layer. The inner layer is maltodextrin-esterified witch hazel composite material, aloe extract and vitamin E, and the outer layer is gum arabic-nano zeolite and hyaluronic acid.

[0072] The matrix comprises the following raw materials in parts by weight: 70 parts of Yellow River sand, 15 parts of volcanic mud, and 3 parts of green tea powder; the inner layer of the microcapsule comprises the following raw materials in parts by weight: 8 parts of maltodextrin-esterified witch hazel composite material, 3 parts of aloe extract, and 1 part of vitamin E; the outer layer of the microcapsule comprises the following raw materials in parts by weight: 8 parts of gum arabic-nano zeolite and 2 parts of hyaluronic acid.

[0073] The preparation methods of the maltodextrin-esterified witch hazel composite material and gum arabic-nano zeolite in this embodiment are the same as those in Example 1. The preparation method of the sand therapy mineral sand for improving skin inflammation in this embodiment is the same as that in Example 1.

[0074] Example 3: This example discloses a sand therapy mineral sand for improving skin inflammation, which is composed of a matrix and microcapsules. The matrix is ​​Yellow River sand, volcanic mud and green tea powder. The microcapsules include an inner layer and an outer layer. The inner layer is maltodextrin-esterified witch hazel composite material, aloe extract and vitamin E, and the outer layer is gum arabic-nano zeolite and hyaluronic acid.

[0075] The matrix comprises the following raw materials in parts by weight: 80 parts of Yellow River sand, 20 parts of volcanic mud, and 5 parts of green tea powder; the inner layer of the microcapsule comprises the following raw materials in parts by weight: 12 parts of maltodextrin-esterified witch hazel composite material, 5 parts of aloe extract, and 2 parts of vitamin E; the outer layer of the microcapsule comprises the following raw materials in parts by weight: 10 parts of gum arabic-nano zeolite and 3 parts of hyaluronic acid.

[0076] The preparation methods of the maltodextrin-esterified witch hazel composite material and gum arabic-nano zeolite in this embodiment are the same as those in Example 1. The preparation method of the sand therapy mineral sand for improving skin inflammation in this embodiment is the same as that in Example 1.

[0077] Example 4: This example discloses a sand therapy mineral sand for improving skin inflammation, which is composed of a matrix and microcapsules. The matrix is ​​Yellow River sand, volcanic mud and green tea powder. The microcapsules include an inner layer and an outer layer. The inner layer is maltodextrin-esterified witch hazel composite material, aloe extract and vitamin E, and the outer layer is gum arabic-nano zeolite and hyaluronic acid.

[0078] The matrix comprises the following raw materials in parts by weight: 75 parts of Yellow River sand, 17.5 parts of volcanic mud, and 4 parts of green tea powder; the inner layer of the microcapsule comprises the following raw materials in parts by weight: 10 parts of maltodextrin-esterified witch hazel composite material, 4 parts of aloe extract, and 1.5 parts of vitamin E; the outer layer of the microcapsule comprises the following raw materials in parts by weight: 9 parts of gum arabic-nano zeolite and 2.5 parts of hyaluronic acid.

[0079] The preparation methods of the maltodextrin-esterified witch hazel composite material and gum arabic-nano zeolite in this embodiment are the same as those in Example 1. The preparation method of the sand therapy mineral sand for improving skin inflammation in this embodiment is the same as that in Example 1.

[0080] Control group 1: This embodiment discloses a sand therapy mineral sand for improving skin inflammation, which is composed of a matrix and microcapsules. The matrix is ​​Yellow River sand, volcanic mud and green tea powder. The microcapsules include an inner layer and an outer layer. The inner layer is aloe extract and vitamin E, and the outer layer is gum arabic-nano zeolite and hyaluronic acid.

[0081] The matrix comprises the following raw materials in parts by weight: 72 parts of Yellow River sand, 16 parts of volcanic mud, and 3 parts of green tea powder; the inner layer of the microcapsule comprises the following raw materials in parts by weight: 3 parts of aloe extract and 1 part of vitamin E; the outer layer of the microcapsule comprises the following raw materials in parts by weight: 10 parts of gum arabic-nano zeolite and 2 parts of hyaluronic acid.

[0082] The preparation method of gum arabic-nano zeolite in this embodiment is consistent with that in embodiment 1. The preparation method of sand therapy mineral sand for improving skin inflammation in this embodiment is consistent with that in embodiment 1.

[0083] Control group 2: This embodiment discloses a sand therapy mineral sand for improving skin inflammation, which is composed of a matrix and microcapsules. The matrix is ​​Yellow River sand, volcanic mud and green tea powder. The microcapsules include an inner layer and an outer layer. The inner layer is maltodextrin, aloe extract and vitamin E, and the outer layer is gum arabic-nano zeolite and hyaluronic acid.

[0084] The matrix comprises the following raw materials in parts by weight: 72 parts of Yellow River sand, 16 parts of volcanic mud, and 3 parts of green tea powder; the inner layer of the microcapsule comprises the following raw materials in parts by weight: 11 parts of maltodextrin, 3 parts of aloe extract, and 1 part of vitamin E; the outer layer of the microcapsule comprises the following raw materials in parts by weight: 10 parts of gum arabic-nano zeolite and 2 parts of hyaluronic acid.

[0085] The preparation methods of maltodextrin and gum arabic-nano zeolite in this embodiment are consistent with those in Example 1. The preparation method of sand therapy mineral sand for improving skin inflammation in this embodiment is consistent with that in Example 1.

[0086] Control group 3: This embodiment discloses a sand therapy mineral sand for improving skin inflammation, which is composed of a matrix and microcapsules. The matrix is ​​Yellow River sand, volcanic mud and green tea powder. The microcapsules include an inner layer and an outer layer. The inner layer is esterified witch hazel, aloe extract and vitamin E, and the outer layer is gum arabic-nano zeolite and hyaluronic acid.

[0087] The matrix comprises the following raw materials in parts by weight: 72 parts of Yellow River sand, 16 parts of volcanic mud, and 3 parts of green tea powder; the inner layer of the microcapsule comprises the following raw materials in parts by weight: 11 parts of esterified witch hazel, 3 parts of aloe extract, and 1 part of vitamin E; the outer layer of the microcapsule comprises the following raw materials in parts by weight: 10 parts of gum arabic-nano zeolite and 2 parts of hyaluronic acid.

[0088] The preparation methods of esterified witch hazel and gum arabic-nano zeolite in this embodiment are consistent with those in Example 1. The preparation method of sand therapy mineral sand for improving skin inflammation in this embodiment is consistent with that in Example 1.

[0089] Control group 4: This embodiment discloses a sand therapy mineral sand for improving skin inflammation, which is composed of a matrix and microcapsules. The matrix is ​​Yellow River sand, volcanic mud and green tea powder. The microcapsules include an inner layer and an outer layer. The inner layer is a maltodextrin-esterified witch hazel composite material, aloe extract and vitamin E, and the outer layer is hyaluronic acid.

[0090] The matrix comprises the following raw materials in parts by weight: 72 parts of Yellow River sand, 16 parts of volcanic mud, and 3 parts of green tea powder; the inner layer of the microcapsule comprises the following raw materials in parts by weight: 11 parts of maltodextrin-esterified witch hazel composite material, 3 parts of aloe extract, and 1 part of vitamin E; the outer layer of the microcapsule comprises the following raw materials in parts by weight: 2 parts of hyaluronic acid.

[0091] The preparation method of the maltodextrin-esterified witch hazel composite material in this embodiment is consistent with that in Example 1. The preparation method of the sand therapy mineral sand for improving skin inflammation in this embodiment is consistent with that in Example 1.

[0092] Control group 5: This embodiment discloses a sand therapy mineral sand for improving skin inflammation, which is composed of a matrix and microcapsules. The matrix is ​​Yellow River sand, volcanic mud and green tea powder. The microcapsules include an inner layer and an outer layer. The inner layer is a maltodextrin-esterified witch hazel composite material, aloe extract and vitamin E, and the outer layer is gum arabic and hyaluronic acid.

[0093] The matrix comprises the following raw materials in parts by weight: 72 parts of Yellow River sand, 16 parts of volcanic mud, and 3 parts of green tea powder; the inner layer of the microcapsule comprises the following raw materials in parts by weight: 11 parts of maltodextrin-esterified witch hazel composite material, 3 parts of aloe extract, and 1 part of vitamin E; the outer layer of the microcapsule comprises the following raw materials in parts by weight: 10 parts of gum arabic and 2 parts of hyaluronic acid.

[0094] The preparation methods of the maltodextrin-esterified witch hazel composite material and gum arabic in this embodiment are the same as those in Example 1. The preparation method of the sand therapy mineral sand for improving skin inflammation in this embodiment is the same as that in Example 1.

[0095] Control group 6: This embodiment discloses a sand therapy mineral sand for improving skin inflammation, which is composed of a matrix and microcapsules. The matrix is ​​Yellow River sand, volcanic mud and green tea powder. The microcapsules include an inner layer and an outer layer. The inner layer is a maltodextrin-esterified witch hazel composite material, aloe extract and vitamin E, and the outer layer is nano zeolite and hyaluronic acid.

[0096] The matrix comprises the following raw materials in parts by weight: 72 parts of Yellow River sand, 16 parts of volcanic mud, and 3 parts of green tea powder; the inner layer of the microcapsule comprises the following raw materials in parts by weight: 11 parts of maltodextrin-esterified witch hazel composite material, 3 parts of aloe extract, and 1 part of vitamin E; the outer layer of the microcapsule comprises the following raw materials in parts by weight: 10 parts of nano zeolite and 2 parts of hyaluronic acid.

[0097] The preparation methods of the maltodextrin-esterified witch hazel composite material and nano zeolite in this embodiment are consistent with those in Example 1. The preparation method of the sand therapy mineral sand for improving skin inflammation in this embodiment is consistent with that in Example 1.

[0098] Test verification:

[0099] 1. In vitro release experiment: Prepare a phosphate buffer solution with a pH of 5.5 to simulate the skin environment, and perform the release experiment at 37°C using the dialysis bag method. Sampling is performed at 0h, 0.5h, 1h, 2h, 4h, 8h, 12h, and 24h, and the release is measured using a UV spectrophotometer.

[0100] 2. Anti-inflammatory performance test: Prepare a cell culture plate, use RAW264.7 macrophage cell line, induce inflammation by lipopolysaccharide (LPS), add the liquid extract extracted from the sand therapy mineral sand prepared in the example group and the control group with phosphate buffered saline to the cell culture plate, and use enzyme-linked immunosorbent assay to detect the expression levels of inflammatory factors TNF-α and IL-6.

[0101] Table 1 In vitro release test data (release rate %)

[0102]

[0103] Table 2 Test data of inflammatory factor expression level

[0104] Group <![CDATA[TNF-α expression level ( pg / mL)]]> <![CDATA[IL-6 expression level ( pg / mL)]]> Example 1 42.5 36.4 Example 2 46.8 40.2 Example 3 45.2 38.6 Example 4 44.1 37.8 Control group 1 82.4 76.8 Control group 2 75.6 70.2 Control group 3 77.8 72.4 Control group 4 88.9 82.6 Control group 5 85.2 79.4 Control group 6 86.7 80.8

[0105] From the in vitro release experimental data, Examples 1 to 4 showed a sustained release effect that was significantly better than that of the control group. Example 1 performed best, reaching a release rate of 98.4% at 24 hours, while the release rates of Control Groups 1 to 6 were only between 54% and 70%. Especially within the first 8 hours, the Example group showed a sustained release characteristic, and the release curve was relatively gentle, which shows that the cage structure of the maltodextrin-esterified witch hazel composite material does play a good encapsulation role. The lack of any key component in the control group (such as the lack of maltodextrin-esterified witch hazel composite material in Control Group 1 and the lack of gum arabic-nano zeolite in Control Group 4) resulted in a significant decrease in release performance, indicating the importance of the synergistic effect of each component to the sustained release of the drug.

[0106] From the perspective of inflammatory factor expression levels, the example group also showed advantages in inhibiting the expression of TNF-α and IL-6. The TNF-α and IL-6 levels of Example 1 were 42.5pg / mL and 36.4pg / mL, respectively, which were about 50% lower than those of the control group (TNF-α: 75-89pg / mL, IL-6: 70-83pg / mL). This shows that the example formula can more effectively deliver anti-inflammatory active ingredients to the target site through an optimized drug delivery system. It is particularly noteworthy that the levels of inflammatory factors in the control group 5 lacking nano-zeolites and the control group 6 with only nano-zeolites were significantly increased, which verifies the hypothesis that the mechanism by which nano-zeolites adsorb inflammatory factors through electrostatic effects.

[0107] Finally, it should be noted that: Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A sand therapy mineral sand for improving skin inflammation, characterized in that: The microcapsule is composed of a matrix and microcapsules. The matrix is ​​Yellow River sand, volcanic mud and green tea powder. The microcapsules include an inner layer and an outer layer. The inner layer is a maltodextrin-esterified witch hazel composite material, aloe extract and vitamin E, and the outer layer is gum arabic-nano zeolite and hyaluronic acid.

2. The sand therapy mineral sand for improving skin inflammation according to claim 1, characterized in that: The matrix comprises the following raw materials in parts by weight: 70 to 80 parts of Yellow River sand, 15 to 20 parts of volcanic mud, and 3 to 5 parts of green tea powder; the inner layer of the microcapsule comprises the following raw materials in parts by weight: 8 to 12 parts of maltodextrin-esterified witch hazel composite material, 3 to 5 parts of aloe extract, and 1 to 2 parts of vitamin E; the outer layer of the microcapsule comprises the following raw materials in parts by weight: 8 to 10 parts of gum arabic-nano zeolite and 2 to 3 parts of hyaluronic acid.

3. The sand therapy mineral sand for improving skin inflammation as claimed in claim 2, characterized in that: The preparation method of the maltodextrin-esterified witch hazel composite material comprises: S11. Place the witch hazel extract in a three-necked flask, then add citric acid and anhydrous ethanol to obtain a mixture A, reflux the mixture A in a water bath at 70 to 80° C. for 4 to 5 hours to obtain a mixture B; S12. The mixture B is filtered to obtain a mixture C, the mixture C is washed with anhydrous ethanol for 3 to 4 times to obtain a washing D, and the washing D is vacuum dried at 60 to 70° C. for 12 to 20 hours to obtain esterified witch hazel; S13. adding maltodextrin to deionized water to obtain a maltodextrin solution, adjusting the pH of the maltodextrin solution to 5.5 to 6.0 to obtain a maltodextrin solution after adjusting the pH, and heating the maltodextrin solution after adjusting the pH to 60 to 70° C. to obtain a heated maltodextrin solution; S14. Adding the esterified witch hazel to the heated maltodextrin solution to obtain a first mixed solution, stirring the first mixed solution with a high-speed disperser at 3000-4000 r / min for 30-40 min to obtain a second mixed solution, stirring the second mixed solution at 60-70° C. for 2-3 h to obtain a third mixed solution, and cooling the third mixed solution to room temperature to obtain a fourth mixed solution; S15. freeze-dry the fourth mixed solution for 12 to 24 hours to obtain a maltodextrin-esterified witch hazel composite material.

4. The sand therapy mineral sand for improving skin inflammation as claimed in claim 2, characterized in that: The preparation method of the gum arabic-nano zeolite comprises: S21. Add nano zeolite to deionized water, then ultrasonicate for 30 to 40 minutes to obtain a nano zeolite dispersion, add sodium chloride solution to the nano zeolite dispersion to obtain a mixed solution, ultrasonicate the mixed solution for 15 to 20 minutes to obtain a mixed solution after ultrasonication, centrifuge the mixed solution after ultrasonication at 8000 to 9000 r / min for 10 to 15 minutes to obtain a centrifuged mixture, wash the centrifuged mixture with anhydrous ethanol for 3 to 4 times to obtain a washed mixture, and vacuum dry the washed mixture at 60 to 70° C. for 12 to 24 hours to obtain a pretreated nano zeolite; S22. Adding gum arabic to deionized water, stirring for 2 to 3 hours to obtain a gum arabic solution, adjusting the pH of the gum arabic solution to 6.5 to 7.0 to obtain a pH-adjusted gum arabic solution, and filtering the pH-adjusted gum arabic solution to obtain a filtrate; S23. Slowly add the pretreated nano zeolite to the filtrate, then ultrasonicate for 30 to 40 minutes to obtain solution A, stir solution A at 50 to 60° C. and 500 to 600 r / min for 2 to 3 hours to obtain solution B, adjust the pH of solution B to 7.0 to 7.5 to obtain solution C, stir solution C for 1 to 2 hours to obtain solution D, centrifuge solution D at 6000 to 7000 r / min for 15 to 20 minutes to obtain a supernatant; S24. Freeze-dry the supernatant for 24 to 48 hours to obtain gum arabic-nano zeolite.

5. A method for preparing sand therapy mineral sand for improving skin inflammation, which is used to prepare the sand therapy mineral sand for improving skin inflammation as claimed in any one of claims 1 to 4, characterized in that: The method comprises the following steps: S31. Passing the Yellow River sand through a 100-120 mesh sieve to obtain sieved Yellow River sand, drying the sieved Yellow River sand at 100-120° C. for 4-5 hours to obtain dried Yellow River sand, and cooling the dried Yellow River sand to room temperature to obtain pretreated Yellow River sand; S32. crushing the volcanic mud through a 200-220 mesh sieve to obtain sieved volcanic mud, drying the sieved volcanic mud at 105-110° C. for 2-3 hours to obtain dried volcanic mud, and cooling the dried volcanic mud to room temperature to obtain pretreated volcanic mud; S33. The green tea powder is sieved through a 200-220 mesh sieve to obtain pretreated green tea powder; S34. dissolving the maltodextrin-esterified witch hazel composite material in deionized water, then adding aloe vera extract to obtain solution A, stirring solution A at 500-600 r / min for 30-40 min to obtain stirred solution A, adding vitamin E to the stirred solution A to obtain solution B, ultrasonicating solution B for 15-30 min to obtain ultrasonicated solution B, adjusting the pH of ultrasonicated solution B to 6.5-7.0 to obtain an inner core material solution; S35. The gum arabic-nano zeolite is dispersed in deionized water and ultrasonically treated for 20 to 30 minutes to obtain a gum arabic-nano zeolite dispersion, a hyaluronic acid solution is added to the gum arabic-nano zeolite dispersion to obtain a solution C, the pH of the solution C is adjusted to 7.0 to 7.5 to obtain a solution D, and the solution D is stirred at 400 to 500 r / min for 30 minutes to obtain an outer coating material; S36. The inner core material solution is added dropwise to the outer coating material at a rate of 2 to 3 mL / min, and stirred at 800 to 900 r / min for 2 to 3 h to obtain a solution E, and the solution E is spray-dried at an inlet temperature of 155 to 165° C., an outlet temperature of 75 to 85° C., an atomization pressure of 0.2 to 0.3 MPa, and a feed rate of 15 to 18 mL / min to obtain microcapsules; S37. The pretreated Yellow River sand and the pretreated volcanic mud are mixed and stirred at high speed for 5 to 10 minutes to obtain a first mixture, the pretreated green tea powder is added to the first mixture to obtain a second mixture, and the second mixture is further mixed for 3 to 5 minutes to obtain a mixed matrix; S38. Sprinkle the microcapsules evenly on the surface of the mixed matrix, and stir and mix at a low speed for 10 to 15 minutes to obtain a third mixture, pass the third mixture through an 80 to 90 mesh sieve to obtain a sieved third mixture, and vacuum dry the sieved third mixture at 50 to 60° C. for 2 to 3 hours to obtain sand therapy mineral sand for improving skin inflammation.

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