Traditional Chinese medicine compound for treating chicken gout as well as preparation method and application of traditional Chinese medicine compound

Abstract

The invention discloses a traditional Chinese medicine compound for treating chicken gout and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 3 to 13 parts of radix clematidis, 1 to 11 parts of liquorice root, 10 to 20 parts of semen plantaginis, 5 to 15 parts of radix astragali seu hedysari and 3 to 13 parts of radix polygonati officinalis. The traditional Chinese medicine compound disclosed by the invention has a good effect of treating the chicken gout, particularly can reduce the uric acid level of chickens and improve the production performance of the chickens, and can be used for treating the chicken gout.

Description

Technical Field

[0001] The invention relates to the technical field of veterinary drugs, and in particular to a traditional Chinese medicine compound for treating chicken gout, and a preparation method and application thereof. Background Art

[0002] Gout is a common metabolic disease in chicken farming. It is caused by elevated serum urate levels or metabolic disorders, and is manifested by the deposition of urate in the internal organs or joints, leading to multiple organ failure or arthritis. The disease can occur all year round, with laying hens and broilers being the most susceptible. In recent years, my country's poultry industry has developed rapidly. Gout in chickens generally does not attract the attention of breeders before the onset of the disease. Therefore, once the disease occurs in farms, it will seriously affect the growth and development of chickens and production performance, and even cause the death of chickens. According to relevant breeding companies, the mortality rate of gout in chickens is as high as 30%-50%, so the prevention and treatment of gout in chickens is of great significance.

[0003] There are four main factors that cause gout in chickens, namely feed factors, management factors, disease factors and drug factors. A high-calcium and high-protein diet will increase the urate level in chickens, and high-density breeding and lack of exercise will reduce the metabolism of urate. Some pathogenic bacteria that are tropistic to renal cells, such as renal infectious bronchitis virus, avian influenza, chicken astrovirus, etc., as well as the abuse of antibiotics, will lead to decreased renal function in chickens. The prevention and treatment of gout in chickens requires strengthened management, rational use of drugs and timely treatment. Some scholars believe that adding methionine or ammonium sulfate to feed can protect chicken kidneys or reduce the pH of chicken uric acid, but there is currently a lack of relevant evidence on whether ammonium sulfate can reduce the pH of chicken uric acid. Although adding methionine to feed can protect the kidneys, it is still uncertain whether it is effective for gout. Baking soda is a common human uric acid-lowering drug, but the actual effect is minimal. Therefore, there is still a lack of strong evidence for the method of adding baking soda to feed to treat gout in chickens. Traditional Chinese medicine compound is a safe and reliable choice. Wulingsan has the effect of protecting the liver and kidney, but there is still a lack of research evidence on whether it is effective for gout in chickens. Centella asiatica oral liquid has a good effect on the treatment of gout in chickens, and the effect is seen in 3 days, but it is not clear whether it can cure gout in chickens and restore the production performance of chickens. In addition, the composition of this product is unclear, which limits further research. Other cases of Chinese herbal compound treatment of gout in chickens have been reported, but economic factors are not considered and the effect is slow, so it is difficult to popularize. In short, there is a serious lack of exclusive veterinary products for gout in chickens, and the development of related drugs is limited. There is an urgent need for the development and research of related Chinese veterinary products.

[0004] At present, the Chinese invention patent with publication number CN105748581A discloses a Chinese medicine composition for preventing and treating gout in chickens, which comprises, by weight, 1-5 parts of Lygodium japonicum, 1-5 parts of Lygodium japonicum, 0.5-3 parts of Aristolochia guanylodis, 0.5-5 parts of Pyrrosia odoratum, 0.5-3 parts of Dianthus superbus, 0.5-3 parts of Lonicera japonica, and 0.1-3 parts of Licorice. However, the preparation of the prescription is complicated, the price is high, and the practical application potential is low.

[0005] At present, the Chinese invention patent with publication number CN108576472A discloses a chicken feed for preventing and treating gout in chickens and its preparation method, which is characterized by comprising the following raw materials by weight: 30-50 parts of corn flour, 30-50 parts of pine needle powder, 10-20 parts of wheat, 15-25 parts of soybeans, 12-15 parts of rapeseed cake, 10-15 parts of wheat bran, 10-15 parts of bean cake, 10-15 parts of fine rice bran, 8-15 parts of alfalfa powder, 0.5-1 parts of shell powder, 0.5-1 parts of compound multivitamins, and 1-3 parts of Chinese herbal medicine additives. However, the formula has many components and high price, and there is no clear evidence that it is effective in preventing and treating gout in chickens. Summary of the invention

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a Chinese medicinal compound for treating chicken gout and a preparation method thereof.

[0007] To achieve the above purpose, the present invention designs a traditional Chinese medicine compound for treating chicken gout. The raw materials of the traditional Chinese medicine compound include 3-13 parts of clematis, 1-11 parts of licorice, 10-20 parts of plantain seeds, 5-15 parts of astragalus, and 3-13 parts of polygonatum.

[0008] Furthermore, the raw materials of the traditional Chinese medicine compound include 6-10 parts of clematis, 4-8 parts of liquorice, 13-17 parts of plantain seeds, 8-12 parts of astragalus, and 6-10 parts of polygonatum odoratum by weight.

[0009] Furthermore, the raw materials of the Chinese medicine compound include 8 parts of Clematis chinensis, 6 parts of Licorice, 15 parts of Plantago, 10 parts of Astragalus, and 8 parts of Polygonatum odoratum by weight.

[0010] The present invention also provides a method for preparing the above-mentioned Chinese medicinal compound for treating chicken gout, comprising the following steps:

[0011] 1) Weigh clematis root, liquorice, plantain seed, astragalus and polygonatum according to the above weight ratio, soak in water, heat to boiling over high heat, pour out the first decoction after boiling, add warm water and heat to obtain the second decoction;

[0012] 2) Mixing the two obtained medicinal solutions to obtain a Chinese medicinal compound.

[0013] The present invention also provides an application of the above-mentioned Chinese medicinal compound for treating chicken gout in preparing a Chinese medicinal preparation for heat stress of livestock and poultry.

[0014] The present invention also provides a traditional Chinese medicine preparation, which comprises the traditional Chinese medicine compound.

[0015] Furthermore, the traditional Chinese medicine preparation also includes pharmaceutically acceptable excipients.

[0016] Furthermore, the auxiliary material is any one or more of vitamin C, sorbitol, mannitol, xylitol, fructose, amino acids, meglumine, dextrin, magnesium stearate and sucrose.

[0017] Furthermore, the Chinese medicine preparation is an oral liquid or granules.

[0018] The pharmacological properties of the present invention are as follows:

[0019] The compound of the present invention mainly dispels wind and dampness, promotes blood circulation and activates collaterals, and at the same time takes into account tonifying qi and nourishing yin, and promoting diuresis and removing dampness. The compatibility principle of "monarch, minister, assistant and messenger" is followed, with Clematis as the monarch drug to dispel wind and dampness, promote blood circulation and activate collaterals; Astragalus and Plantago as the minister drugs, which can tonify qi and raise yang, consolidate the exterior and stop sweating, promote diuresis and reduce swelling, promote diuresis and remove dampness, clear away heat and improve eyesight, and remove phlegm; Polygonatum odoratum is the adjuvant drug, which can nourish yin and moisten dryness, promote body fluid and quench thirst; Licorice is the messenger drug, which can tonify the spleen and replenish qi, clear away heat and detoxify, relieve urgency and stop pain, and harmonize various drugs.

[0020] Beneficial effects of the present invention:

[0021] 1. The Chinese medicinal compound of the present invention has a good effect of treating gout in chickens, especially can reduce serum uric acid level and restore the production performance of chickens. It can be used to treat gout in chickens.

[0022] 2. The Chinese medicine compound of the present invention has a rapid effect. The uric acid level can be significantly reduced after 3 days of administration, and the growth performance of chickens can be restored after 5-7 days of treatment. The drug can be stopped when the gout of chickens subsides. The drug composition of the prescription is simple, with only 5 Chinese medicines, and the medicinal materials used are affordable. The price of each dose in a Chinese medicine hospital is less than 5 yuan, and one dose of medicine can be used for 100-1000 chickens. The oral liquid prepared by the prescription has a rapid effect, is easy to absorb, and is simple to prepare. The granules prepared by the prescription are easy to store, have good palatability, and are convenient to take. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a graph showing the recovery of growth performance of gout-stricken chickens after 5-7 days of compound treatment;

[0024] In the figure, (a) a schematic diagram of the weight change of chickens within one week of drug administration; (b) a schematic diagram of the average daily feed intake; (c) a schematic diagram of the average daily weight gain; (d) feed-to-weight ratio (F / G). A, blank control group; B, model group; C, F1 treatment group; D, F2 treatment group; E, F3 treatment group.

[0025] Figure 2 The renal function indicators of gout-stricken chickens were restored after 3 days of compound treatment.

[0026] In the figure, (a) a schematic diagram of the serum creatinine level of chickens after one week of administration; (b) a schematic diagram of the serum urea nitrogen level of chickens after one week of administration; (c) a schematic diagram of the serum uric acid level of chickens after one week of administration. A, blank control group; B, model group; C, F1 treatment group; D, F2 treatment group; E, F3 treatment group. DETAILED DESCRIPTION

[0027] The present invention is further described in detail below in conjunction with specific embodiments so that those skilled in the art can understand.

[0028] Example 1

[0029] The raw material composition of the traditional Chinese medicine compound 1 for treating chicken gout is: 8g of Clematidis, 6g of Licorice, 15g of Plantago, 10g of Astragalus, and 8g of Polygonatum.

[0030] The above-mentioned Chinese medicinal materials were purchased from Wuhan Zhihe Qingqiang Pharmacy Co., Ltd. and used in subsequent experiments after accurate weighing.

[0031] The preparation method of the above-mentioned Chinese medicine compound 1 comprises the following steps:

[0032] 1) Weigh 8g of Clematidis, 6g of Licorice, 15g of Plantago, 10g of Astragalus, and 8g of Polygonatum. Soak them in cold water (distilled water) for 20min, with the water surface 2-3cm above the medicine (or according to the material-liquid ratio of 1:10, w / v), heat to boiling over high heat, and pour out the first decoction after heating for 35min from the boiling point, then add an appropriate amount of warm water and heat for 25min to obtain the second decoction.

[0033] 2) Mixing the two obtained medicinal solutions (water decoctions) to obtain the Chinese medicinal compound 1.

[0034] Example 2

[0035] The Chinese medicine compound 2 prepared in this example is basically the same as that in Example 1, except that:

[0036] The raw materials of the Chinese medicine compound 2 include 10 parts of clematis, 6 parts of liquorice, 15 parts of plantain seeds, 10 parts of astragalus, and 8 parts of polygonatum odoratum by weight.

[0037] Example 3

[0038] The Chinese medicine compound 3 prepared in this example is basically the same as that in Example 1, except that:

[0039] The raw materials of the above-mentioned Chinese medicine compound 3 include 8 parts of clematis, 6 parts of liquorice, 15 parts of plantain seeds, 10 parts of astragalus, and 10 parts of polygonatum odoratum by weight.

[0040] Example 4

[0041] A Chinese medicine compound granule is prepared by the following steps:

[0042] The Chinese medicine compound 1 obtained in Example 1 is heated and concentrated to a thick paste (5-10g), and 50g of dextrin and sucrose are weighed and mixed as auxiliary materials. The thick paste is first slowly poured into the auxiliary materials of a large evaporating dish, and mixed to form a soft material (the softness and hardness of the soft material should be appropriate, so that it can be "held in a ball by hand and scattered by light pressure"). Then, 18-mesh stainless steel sieve is used for extrusion granulation, and the granules are pressed into square porcelain plates. Put it in a drying oven and dry it at 40-60°C for about 1h. Take it out and weigh it, and then use 12-mesh and 60-mesh stainless steel sieves to granulate it. Finally, weigh it again and calculate the granule yield. The granules have good animal palatability, are easy to store, and can be directly added to feed.

[0043] The Chinese medicine compound prepared in the above examples has the following verification effects:

[0044] 1. Verify the side effects of Chinese medicine compound 1

[0045] The Chinese medicine compound 1 prepared in Example 1 was gavaged to rats at a dosage of 30 g / kg, and the median lethal dose of mice was calculated 14 days after administration; the results showed that all rats had normal diet and normal bowel movements, and no animals died until the end of the experiment 14 days after administration, and no abnormal symptoms appeared in the animals. The skin, fur, eyes and mucous membranes, secretions, and excretions were normal, and there were no abnormalities in the respiratory, circulatory, autonomic and central nervous systems, and no symptoms such as tremor, convulsion, salivation, diarrhea, sluggishness, lethargy and coma.

[0046] The acute oral toxicity tolerance dose of this prescription for female and male SD rats is greater than 30g / kg body weight, and its LD 50 Greater than 30g / kg body weight. According to the acute toxicity (LD 50 )Dose grading, compound 1 is non-toxic.

[0047] 2. Verify the effectiveness of Chinese medicine compound 1 to 3

[0048] (1) Experimental Materials

[0049] 1.1 Drugs: Chinese herbal medicines 1, 2, and 3 were concentrated to 1 g / mL using a rotary evaporator; normal saline; creatinine (CREA), urea (UREA), and uric acid (UA) detection kits, Shenzhen Mindray Bio-Medical Electronics Co., Ltd.

[0050] 1.2 Animals: 15-18 day-old white-feathered broiler chickens (♀25; ♂25) were purchased from Ezhou Lianghuchun Agriculture and Animal Husbandry Co., Ltd. This experiment was approved by the Scientific Ethics Committee of Huazhong Agricultural University, with the ethics number HZAUCH-2022-0018.

[0051] 1.3 Feed: broiler basic feed, Huanggang Oriental Hope Animal Nutrition Food Co., Ltd. Soybean meal, high calcium stone powder, Jiahui Feed.

[0052] 1.4 Equipment: Fully automatic biochemical analyzer (BS-240), Shenzhen Mindray Bio-Medical Electronics Co., Ltd. Digital display closed electric furnace, Changsha Mingjie Instrument Co., Ltd.

[0053] (2) Experimental methods

[0054] 2.1 Grouping: 50 chickens were divided into 5 groups, with 10 in each group. Group A was the control group, fed with basic feed and drinking tap water; Group BE was the model group, which was fed with high calcium and high protein feed during the experiment. The high calcium and high protein feed used was prepared by 35% basic diet, 10% stone powder, and 55% soybean meal, with a total protein content of more than 30% and a total calcium content of more than 4%.

[0055] 2.2 Administration: After the gout model was successfully established, 1% of compound F1, F2 and F3 (the concentration of the compound mother solution was 1 g / mL) was added to the drinking water of the CD group for 7 consecutive days; the AB group drank tap water.

[0056] 2.3 Growth performance indicators: During the modeling period, the body weight of each chicken was weighed on days 0 / 7 / 14 / 21, and the feed intake, water intake and body weight gain of each group of broilers were measured every week to calculate the average daily feed intake (ADFI g / d), average daily water intake (ADWI g / d), average daily weight gain (ABW g / d) and feed-to-weight ratio (F / G); after the start of drug administration, the body weight, feed intake and water intake of each chicken were weighed on days 1 / 3 / 5 / 7 of drug administration to calculate the average daily feed intake (ADFI g / d), average daily weight gain (ABWg / d) and feed-to-weight ratio (F / G).

[0057] 2.4 Observation of behavioral symptoms in chickens: During the modeling period, the incidence of gout and the number of deaths in each group of chickens during the experiment were recorded weekly; during the drug administration period, the ankle circumference of each group of chickens was measured every day on the 1st / 3rd / 5th / 7th day respectively.

[0058] 2.5 Serum biochemistry: During the modeling period, blood was collected from the wing vein on days 0 / 7 / 14 / 21 and after the start of drug administration, and on days 1 / 3 / 5 / 7 of drug administration. The chicken wings were spread out to expose the wing root vein, and the No. 7 needle was tilted at 30° to puncture the blood vessel. 2 mL of blood was collected in a vacuum blood collection and coagulation tube, and compression was performed to stop bleeding after completion. The blood sample was allowed to stand at room temperature for 2 hours to separate the chicken serum, and centrifuged at 6000rpm×3min to separate the serum. The activity or content of uric acid (UA), urea (UREA), and creatinine (CREA) was detected by a fully automatic serum biochemical analyzer.

[0059] (3) Experimental results

[0060] After 5 days of treatment with Chinese herbal compound F1, F2 and F3, the growth performance of gout chickens gradually recovered. After treatment with Chinese herbal compound F1, F2 and F3, the average weight of chickens gradually recovered and increased compared with the model group (p>0.05)( Figure 1 a), there was no significant difference in diet (p>0.05)( Figure 1 b). From the perspective of average daily weight gain, by the 7th day, the average daily weight gain of chickens was significantly increased compared with the model group (p<0.05) ( Figure 1 c). In terms of feed-to-weight ratio, the feed-to-weight ratio of chickens treated with Chinese herbal compound F3 (E group) for 5 days was significantly lower than that of the model group (p<0.05) ( Figure 1 d), after 7 days of treatment, the feed-to-weight ratio of groups D and E was significantly reduced compared with the model group (p<0.05). In conclusion, these results indicate that the Chinese herbal formulas F1, F2 and F3 can restore the growth performance of gout-stricken chickens on days 5-7 of treatment.

[0061] The renal function of chickens treated with Chinese herbal compound F1, F2 and F3 for 7 days was restored. The uric acid levels of experimental groups D and E were significantly reduced compared with model group B (P<0.05) after only one day of treatment with F2 and F3 ( Figure 2 c), the uric acid level of the F1 group (group C) treated with low dose for 7 days was also significantly lower than that of the model group B (P<0.05). After 3 days of treatment, the uric acid levels of the experimental groups D and E were significantly lower than those of the model group B (P<0.05) ( Figure 2 ab).

[0062] In summary, the results suggest that the Chinese herbal compound F1, F2 and F3 have a rapid effect in lowering uric acid, taking effect after one day of administration, and can significantly restore the renal function of gout-affected chickens after 3-5 days of treatment.

[0063] In summary, the present invention can be used as a traditional Chinese medicine compound for treating chicken gout.

[0064] Although the above embodiments have been described in detail, they are only a part of the embodiments of the present invention, not all of them. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all belong to the protection scope of the present invention.

Claims

1. A Chinese medicinal compound for treating gout in chickens, characterized in that: The raw materials of the traditional Chinese medicine compound include 3-13 parts of clematis, 1-11 parts of liquorice, 10-20 parts of plantain seeds, 5-15 parts of astragalus, and 3-13 parts of polygonatum odoratum by weight.

2. The Chinese medicinal compound for treating gout in chickens according to claim 1, characterized in that: The raw materials of the traditional Chinese medicine compound include 6-10 parts of clematis, 4-8 parts of liquorice, 13-17 parts of plantain seeds, 8-12 parts of astragalus, and 6-10 parts of polygonatum odoratum by weight.

3. The Chinese medicinal compound for treating gout in chickens according to claim 1, characterized in that: The raw materials of the traditional Chinese medicine compound include 8 parts of clematis, 6 parts of liquorice, 15 parts of plantain seeds, 10 parts of astragalus, and 8 parts of polygonatum odoratum by weight.

4. A method for preparing a Chinese medicinal compound for treating gout in chickens according to any one of claims 1 to 3, characterized in that: The following steps are involved: 1) Weigh clematis root, liquorice, plantain seed, astragalus and polygonatum according to the above weight ratio, soak them in water, heat them over high heat until boiling, pour out the first decoction after boiling, and then add warm water to heat to obtain the second decoction; 2) Mixing the two obtained medicinal solutions to obtain a Chinese medicinal compound.

5. Use of the Chinese medicinal compound for treating chicken gout according to any one of claims 1 to 3 in preparing a Chinese medicinal preparation for treating chicken gout.

6. A Chinese medicine preparation, characterized in that: The Chinese medicine preparation comprises the Chinese medicine compound according to any one of claims 1 to 3.

7. The Chinese medicine preparation according to claim 6, characterized in that: The traditional Chinese medicine preparation also includes pharmaceutically acceptable excipients.

8. The Chinese medicine preparation according to claim 7, characterized in that: The auxiliary material is any one or more of vitamin C, sorbitol, mannitol, xylitol, fructose, amino acids, meglumine, dextrin, magnesium stearate and sucrose.

9. The preparation method according to claim 6, 7 or 8, characterized in that: The Chinese medicine preparation is an oral liquid or granules.

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