Compound essential oil for relaxing tendons, activating collaterals and relieving pain and processing method thereof

Abstract

The invention discloses compound essential oil for relaxing tendons, activating collaterals and relieving pain. The compound essential oil is prepared from the following components in percentage by volume: 10-30% of pepper essential oil, 10-20% of melaleuca alternifolia essential oil, 5-9% of ginger essential oil, 3-5% of mint essential oil, 3-5% of cinnamon essential oil, 1-3% of star anise essential oil, 10-20% of jojoba oil and the balance of ethanol and purified water. The compound essential oil disclosed by the invention is processed by the following steps: dissolving the chili essential oil in ethanol and purified water, adding the melaleuca alternifolia essential oil, the ginger essential oil, the mint essential oil, the cinnamon essential oil, the star anise essential oil and the jojoba oil, and mixing to form an oil agent which is fragrant in smell, light red, free of precipitates and transparent. The oil agent for external use can promote blood circulation and play a role in easing pain, and does not have any toxic or side effect.

Description

Technical Field

[0001] The invention belongs to the technical field of compound essential oil preparation, and in particular relates to a compound essential oil for relaxing muscles and tendons, activating blood circulation and relieving pain, and a processing method thereof. Background Art

[0002] Shujin Huoluo is a term in traditional Chinese medicine, which refers to unblocking tendons and stretching muscles, promoting blood circulation, and achieving the purpose of relieving pain and strengthening physical fitness. Among them, Shujin refers to improving tendons, relaxing muscles, relieving muscle stiffness and pain, and increasing body flexibility and comfort; Huoluo refers to strengthening the physical fitness by promoting blood circulation, preventing neurasthenia, joint pain and a series of other diseases.

[0003] Compound essential oils refer to formula essential oils that are prepared by blending multiple single essential oils with base oils based on the characteristics of essential oils and are ready for immediate use. If the blended essential oil is to be applied to the skin, base oil is also needed to blend it. Compound essential oils that are directly applied to the skin are also a mixture of multiple single essential oils and base oils in a certain proportion. Compound essential oils are different from single essential oils, and their specific usage methods are also different in order to achieve different effects. Compound essential oils are often formulated using the volume percentage content of single essential oils. The biggest difference from single essential oils is that they can be used directly on the skin without further dilution.

[0004] The base oils in compound essential oils include jojoba oil, grape seed oil, evening primrose oil, sweet almond oil, rose hip oil, Hypericum oil, wheat germ oil, etc. Among them, some base oils such as jojoba oil are rich in nutrients and not prone to allergies. They can be used on dry skin as well as oily skin. After use, the skin will be refreshed and non-greasy, and it can effectively moisturize, soothe the skin and relieve pain.

[0005] Currently on the market, many compound essential oils claim to promote blood circulation and have pain-relieving effects, but the actual effects are not ideal. Summary of the invention

[0006] The invention aims to provide a compound essential oil for relaxing muscles and tendons, activating blood circulation and relieving pain.

[0007] Another object of the present invention is to provide a method for preparing the compound essential oil for relaxing muscles and tendons, activating blood circulation and relieving pain.

[0008] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0009] The first aspect of the present invention provides a compound essential oil for relaxing muscles and tendons and relieving pain, which is made of the following components in percentage by volume:

[0010] 10%-30% of pepper essential oil, 10%-20% of Melaleuca alternifolia essential oil, 5%-9% of ginger essential oil, 3%-5% of peppermint essential oil, 3%-5% of cinnamon essential oil, 1%-3% of star anise essential oil, 10%-20% of jojoba oil, and the remainder is ethanol and purified water.

[0011] Most preferably, the compound essential oil for relaxing muscles and tendons, activating collaterals and relieving pain is made of the following components in percentage by volume:

[0012] Capsicum essential oil 20.0%, Melaleuca alternifolia essential oil 15.0%, Ginger essential oil 7.0%, Peppermint essential oil 4.0%, Cinnamon essential oil 4.0%, Star anise essential oil 2.0%, Jojoba oil 20.0%, Ethanol 26.6% and Purified Water 1.4%.

[0013] Most preferably, the compound essential oil for relaxing muscles and tendons, activating collaterals and relieving pain is made of the following components in percentage by volume:

[0014] Capsicum essential oil 30.0%, Melaleuca alternifolia essential oil 20.0%, Ginger essential oil 7.0%, Peppermint essential oil 4.0%, Cinnamon essential oil 4.0%, Star anise essential oil 2.0%, Jojoba oil 10.0%, Ethanol 21.85% and Purified Water 1.15%.

[0015] The second aspect of the present invention provides a method for preparing the compound essential oil for relaxing muscles and tendons, activating collaterals and relieving pain, comprising the following preparation steps:

[0016] First, add pepper essential oil to ethanol and purified water according to the ratio to form a mixed solution, and stir for 1 to 3 hours;

[0017] Second, add Melaleuca alternifolia essential oil to the mixture and stir for 1 to 3 hours;

[0018] Third, add ginger essential oil, peppermint essential oil, cinnamon essential oil, star anise essential oil, and jojoba oil to the mixture and stir for 1 to 3 hours;

[0019] Fourth, place the mixture at 2°C to 8°C for 24 to 96 hours;

[0020] Fifth, the mixed solution is centrifuged at 2°C to 8°C with a centrifugal force of 10,000 g to 20,000 g to remove the precipitate and collect the supernatant, which is a transparent oil with a fragrant odor and a light red color without any precipitate.

[0021] The invention provides a method for preparing the main components of the compound essential oil for relaxing muscles and tendons, activating blood circulation and relieving pain.

[0022] A preparation method of the pepper essential oil as a component of the compound essential oil for relaxing muscles and tendons and relieving pain is as follows:

[0023] First, select high-quality red and spicy peppers, remove the pepper stems, and then dry or sun-dry them before crushing them;

[0024] Second, add 95% edible alcohol to the chili powder, with the volume ratio of chili powder to 95% edible alcohol being 1:2, and soak at room temperature for 48 to 96 hours;

[0025] Third, filter the chili powder alcohol soaking liquid through a 100-mesh filter and collect the supernatant;

[0026] Fourth, the leaching solution is sent to a vacuum distillation device to recover ethanol by a method of vacuum distillation and indirect heating;

[0027] Fifth, the extract is concentrated into a thick dark red pepper concentrate with a density of 0.91 kg / L to 0.93 kg / L to obtain pepper essential oil.

[0028] A preparation method of the Melaleuca alternifolia essential oil, a component of the compound essential oil for relaxing muscles and tendons and relieving pain, is as follows:

[0029] First, collect fresh branches and leaves of 1,8-cineole-type Melaleuca alternifolia;

[0030] second, steam distillation of 1,8-cineole-type fresh leaves of Melaleuca alternifolia to obtain Melaleuca alternifolia essential oil;

[0031] Thirdly, the content of 1,8-cineole in the obtained Melaleuca alternifolia essential oil is greater than 80%.

[0032] The components of the compound essential oil for relaxing muscles and tendons and relieving pain, namely ginger essential oil, peppermint essential oil, cinnamon essential oil and star anise essential oil, are prepared by steam distillation:

[0033] First, select high-quality ginger, wash it, dry it, cut it into 1mm to 3mm slices, and air-dry it for 24 hours;

[0034] Second, harvest the above-ground branches and leaves of mint and air-dry them for 24 hours; select dried cinnamon bark and dried star anise;

[0035] Third, place the material on the sieve plate in the distillation pot and pass saturated steam through it for 4 to 12 hours;

[0036] Fourth, collect the distilled water vapor and cool it into an aqueous solution;

[0037] Fifth, the essential oil is separated from the aqueous solution using an essential oil separator.

[0038] The detection index of the compound essential oil for relaxing muscles and tendons, activating collaterals and relieving pain is that the total number of bacteria is less than 10 / ml, the coliform group is less than 3 / ml, the arsenic content is less than 0.1 mg / kg, and mercury and lead shall not be detected.

[0039] The application method of the compound essential oil for relaxing muscles and tendons, activating collaterals and relieving pain prepared by the above technical method is external use, including direct application on the skin and penetration by covering the skin with an oil-soaked film.

[0040] The compound essential oil for relaxing muscles and tendons, activating collaterals and relieving pain prepared by the above technical method has the following effects:

[0041] In the first experiment, the clinical research data of the compound essential oil for relaxing muscles and activating collaterals and relieving pain, which promotes blood circulation and relieves pain, are as follows:

[0042] A clinical study on the effect of the compound essential oil for relaxing muscles, activating collaterals and relieving pain on human pain was conducted using a pain scoring table, wherein 1 to 3 points are mild pain, the patient feels some pain but can still fall asleep at night; 4 to 7 are moderate pain, the patient feels 1 pain and cannot sleep well at night; 8 to 10 points are severe pain, which is too painful and the patient cannot fall asleep at night.

[0043] A total of 759 patients with mild trauma (such as shallow II-degree burns, minor abrasions, and knife wounds) were collected from 56 town clinics or street health service centers in Liaoning Province, Hebei Province, Henan Province, Jiangsu Province, Hunan Province, and Guangxi Zhuang Autonomous Region. The patients ranged in age from 4 to 52 years old, with an average age of 16.7±6.5 years old. There were 462 males and 297 females. With the written consent of the patients or guardians, the wounds were treated with a compound essential oil that relaxes muscles and relieves pain. The changes in the patients' pain were measured using a pain scoring table, and the blood flow of 5 to 7 microvessels in the injured area of ​​the patients was measured using a microcirculation blood detector at a magnification of 320 times.

[0044] Clinical research data show that before treatment with the compound essential oil for relaxing muscles and relieving pain, the pain score of patients with mild trauma was 5.1±1.2, and the microvascular blood flow in the injured area was set to 1.0±0.0; 15 minutes, 30 minutes and 60 minutes after treatment with the compound essential oil for relaxing muscles and relieving pain, the pain score of patients with mild trauma decreased significantly, which were 3.7±0.9, 2.6±0.6 and 1.6±0.5 respectively, and the microvascular blood flow in the injured area was significantly enhanced, which were 1.7±0.3, 1.5±0.3 and 1.3±0.2 respectively; these data were analyzed by their own t-test, and the p values ​​were all less than 0.01. The data show that the compound essential oil for relaxing muscles and relieving pain can promote blood circulation in patients and exert an analgesic effect.

[0045] The second experiment, the animal experimental data of the compound essential oil for relaxing muscles and activating collaterals and relieving pain, which promotes blood circulation and relieves pain, is as follows:

[0046] Thirty 2-month-old SPF SD rats, weighing 200g±10g, were randomly and evenly divided into a normal saline group, a glycerol group, and a compound essential oil group for relaxing muscles and activating collaterals and relieving pain, with 10 rats in each group, ensuring that there were 5 male and 5 female rats in each group.

[0047] The rat hot plate analgesia was used to measure the pain threshold of the rats, that is, the pain sensitivity of the rats to thermal stimulation was evaluated by observing the rats' pain reaction time at different temperatures; the blood flow of 5 to 7 microvessels in the rats' feet was measured using a microcirculation blood detector at a magnification of 320 times.

[0048] The experimental data showed that before treatment with saline, glycerol and the compound essential oil for relaxing muscles and activating collaterals, the pain thresholds of the rats in the saline group, glycerol group and the compound essential oil group were 4.5±1.0 minutes, 4.6±0.8 minutes and 4.4±0.9 minutes, respectively; 15 minutes, 30 minutes and 60 minutes after applying saline, glycerol and the compound essential oil for relaxing muscles and activating collaterals to the four feet of the rats, the pain thresholds of the rats in the saline group did not change significantly, which were 4.6±0.9 minutes, 4.4±0.7 minutes and 4.4±0.8 minutes, respectively, and the pain thresholds of the rats before treatment were compared with the pain thresholds of the rats by t-test. The p values ​​were all greater than 0.05, which was not statistically significant; the pain threshold of the rats in the glycerol group was slightly increased, which was 5.5±1.2 minutes, 5.8±1.3 minutes and 6.3±1.3 minutes, respectively. Compared with the pain threshold of the rats before treatment by t-test, the p values ​​were all greater than 0.05, which was not statistically significant; the pain threshold of the rats in the oil and the compound essential oil groups for relaxing muscles, activating collaterals and relieving pain was significantly increased, which was 7.8±1.0 minutes, 10.2±1.2 minutes and 12.4±1.4 minutes, respectively. Compared with the pain threshold of the rats before treatment by t-test, the p values ​​were all less than 0.01, which was statistically significant.

[0049] Before treatment with normal saline, glycerol and the compound essential oil for relaxing muscles and activating collaterals and relieving pain, the pain thresholds of the rats in the normal saline group, the glycerol group and the compound essential oil group were analyzed by t-test, and the p values ​​were all greater than 0.05, indicating that there was no significant difference in the pain thresholds of the rats in each group before treatment; 15 minutes, 30 minutes and 60 minutes after treatment, the pain thresholds of the rats in the normal saline group and the rats in the glycerol group were analyzed by t-test, and the p values ​​were all greater than 0.05, which were not statistically significant, while the pain thresholds of the rats in the normal saline group or the pain thresholds of the rats in the glycerol group and the compound essential oil group were analyzed by t-test, and the p values ​​were all less than 0.01, which were statistically significant.

[0050] The above experimental data show that the compound essential oil that relaxes muscles and relieves pain has the effect of relieving pain.

[0051] The experimental data also showed that before the treatment with normal saline, glycerol and the compound essential oil for relaxing muscles and activating collaterals and relieving pain, the blood fluidity of the microvessels in the feet of the rats in the normal saline group, the glycerol group and the compound essential oil for relaxing muscles and activating collaterals and relieving pain were all set to 1.0±0.0; 15 minutes, 30 minutes and 60 minutes after the four feet of the rats were smeared with normal saline, glycerol and the compound essential oil for relaxing muscles and activating collaterals and relieving pain, the blood fluidity of the microvessels in the feet of the rats in the normal saline group was calculated by taking the average of the four feet, and there was no significant change, which was 1.1±0.2, 1.0±0.2 and 1.0±0.3 respectively. The blood fluidity of the microvessels in the feet of the rats before treatment was compared with the t-test, and the p values ​​were all greater than 0.05, with no significant difference. Statistical significance: The average blood fluidity of the four feet of the rats in the glycerol group was slightly increased, which were 1.1±0.3, 1.2±0.3 and 1.4±0.4 respectively. Compared with the blood fluidity of the feet of the rats before treatment, the p values ​​were all greater than 0.05, which was not statistically significant; the blood fluidity of the four feet of the rats in the compound essential oil group that relaxes muscles, activates collaterals and relieves pain was significantly increased, which were 3.4±0.7, 5.3±1.0 and 7.1±1.2 respectively. Compared with the blood fluidity of the feet of the rats before treatment, the p values ​​were all less than 0.01, which was statistically significant.

[0052] Before treatment with normal saline, glycerol and the compound essential oil for relaxing muscles and activating collaterals and relieving pain, the blood fluidity of the foot microvessels of the rats in the normal saline group, the glycerol group and the compound essential oil group were analyzed by t-test, and the p values ​​were all greater than 0.05, indicating that there was no significant difference in the blood fluidity of the foot microvessels of the rats in each group before treatment; 15 minutes, 30 minutes and 60 minutes after treatment, the pain threshold of the rats in the normal saline group and the blood fluidity of the foot microvessels of the rats in the glycerol group were analyzed by t-test, and the p values ​​were all greater than 0.05, which were not statistically significant, while the blood fluidity of the foot microvessels of the rats in the normal saline group or the blood fluidity of the foot microvessels of the rats in the glycerol group and the blood fluidity of the foot microvessels of the rats in the compound essential oil group were analyzed by t-test, and the p values ​​were all less than 0.01, which were statistically significant.

[0053] The above experimental data show that the compound essential oil that relaxes muscles and relieves pain can promote blood circulation.

[0054] The results of the preclinical safety evaluation of the compound essential oil for relaxing muscles, activating collaterals and relieving pain prepared by the above-mentioned technical method are as follows:

[0055] Eighteen 7-week-old SPF BALB / c mice weighing 20±1.5g were selected, half of them were male and half were female. Before the experiment, the left, middle and right patches of fur on the back and the front, middle and back patches of fur on the abdomen were shaved off. Afterwards, the shaved mice were smeared with normal saline, glycerin and the compound essential oil for relaxing muscles and activating collaterals and relieving pain, respectively, with each component applied to 2 patches per mouse, 3 times a day, for 30 consecutive days. Afterwards, the application was stopped and the mice were allowed to recover for 30 days. The effects of normal saline, glycerin and the compound essential oil for relaxing muscles and activating collaterals and relieving pain on the skin, eating, drinking, activity, urination, defecation and blood biochemical indicators of the mice were observed.

[0056] On the 31st day of the experiment, which was the first day after stopping the application of the drug, 6 mice were killed, and the remaining 12 mice were killed after the experiment was completed. The killed mice were fixed with 40% formaldehyde solution for 15 days, and the organs of the mice, especially the skin applied with the drug, were removed, embedded in paraffin, sliced, and the changes in each tissue were observed under a microscope.

[0057] The results of the preclinical safety evaluation show that the compound essential oil that relaxes muscles and relieves pain has no toxic side effects when applied externally.

Claims

1. A compound essential oil for relaxing muscles and tendons and relieving pain, characterized in that Made of the following components in percentage by volume: 10%-30% of pepper essential oil, 10%-20% of Melaleuca alternifolia essential oil, 5%-9% of ginger essential oil, 3%-5% of peppermint essential oil, 3%-5% of cinnamon essential oil, 1%-3% of star anise essential oil, 10%-20% of jojoba oil, and the remainder is ethanol and purified water.

2. The compound essential oil for relaxing muscles and tendons and relieving pain according to claim 1, characterized in that The compound essential oil for relaxing muscles and tendons, activating collaterals and relieving pain is made of the following components in percentage by volume: Capsicum essential oil 20%, Melaleuca alternifolia essential oil 15%, Ginger essential oil 7%, Peppermint essential oil 4%, Cinnamon essential oil 4%, Star anise essential oil 2%, Jojoba oil 20%, Ethanol 26.6% and Purified Water 1.4%.

3. The compound essential oil for relaxing muscles and tendons and relieving pain according to claim 1, characterized in that The compound essential oil for relaxing muscles and tendons, activating collaterals and relieving pain is made of the following components in percentage by volume: Capsicum essential oil 30%, Melaleuca alternifolia essential oil 20%, Ginger essential oil 7%, Peppermint essential oil 4%, Cinnamon essential oil 4%, Star anise essential oil 2%, Jojoba oil 10%, Ethanol 21.85% and Purified Water 1.15%.

4. The method for preparing the compound essential oil for relaxing muscles and tendons, activating collaterals and relieving pain according to any one of claims 1 to 3, characterized in that The method comprises the following preparation steps: First, add pepper essential oil to ethanol and purified water according to the ratio to form a mixed solution, and stir for 1 to 3 hours; Second, add Melaleuca alternifolia essential oil to the mixture and stir for 1 to 3 hours; Third, add ginger essential oil, peppermint essential oil, cinnamon essential oil, star anise essential oil, and jojoba oil to the mixture and stir for 1 to 3 hours; Fourth, place the mixture at 2°C to 8°C for 24 to 96 hours; Fifth, the mixed solution is centrifuged at 10000g-20000g at 2°C-8°C, and the supernatant is collected, which is a transparent oil with a fragrant odor, light red color, no precipitation.

5. A compound essential oil for relaxing muscles and tendons and relieving pain, characterized in that The pepper essential oil is prepared by the following method: first, selecting high-quality red and spicy peppers, removing the pepper stems, drying or sun-drying, and then crushing; Second, add 95% edible alcohol to the chili powder, with the volume ratio of chili powder to 95% edible alcohol being 1:2, and soak at room temperature for 48 to 96 hours; Third, filter the chili powder alcohol soaking liquid through a 100-mesh filter and collect the supernatant; Fourth, the leaching solution is sent to a vacuum distillation device to recover ethanol by a vacuum indirect heating method; Fifth, the extract is concentrated into a thick dark red pepper concentrate with a density of 0.91 kg / L to 0.93 kg / L to obtain pepper essential oil.

6. A compound essential oil for relaxing muscles and tendons and relieving pain, characterized in that The essential oil of Melaleuca alternifolia was obtained by steam distillation of fresh leaves and branches of 1,8-cineole-type Melaleuca alternifolia.

7. The Melaleuca alternifolia essential oil of claim 6, characterized in that The content of 1,8-cineole in Melaleuca alternifolia essential oil is greater than 80%.

8. A compound essential oil for relaxing muscles and tendons and relieving pain, characterized in that The ingredients ginger essential oil, peppermint essential oil, cinnamon essential oil and star anise essential oil are prepared by steam distillation: First, wash and dry high-quality ginger, cut into 1 mm to 3 mm slices, and air-dry for 24 hours; Second, harvest the above-ground branches and leaves of mint and air-dry them for 24 hours; Third, pick dried cinnamon bark and dried star anise; Fourth, place the material on the sieve plate in the distillation pot and pass saturated steam into it for 4 to 12 hours; Fifth, collect the distilled water vapor and cool it into an aqueous solution; Sixth, the essential oil is separated from the aqueous solution using an essential oil separator.

9. A compound essential oil for relaxing muscles and tendons and relieving pain, characterized in that Clinical studies and animal experiments have shown that compound essential oils that relax muscles, activate collaterals and relieve pain can promote blood circulation and exert an analgesic effect.

10. A compound essential oil for relaxing muscles and tendons and relieving pain, characterized in that Through preclinical safety evaluation, the compound essential oil that relaxes muscles, activates blood circulation and relieves pain was found to have no toxic side effects when used externally.