Microbial synergistic nitrogen fixation fertilizer based on flavone induction and preparation method thereof

Abstract

The invention provides a microorganism synergistic nitrogen fixation fertilizer based on flavone induction and a preparation method thereof, and belongs to the technical field of agricultural fertilizers. The fertilizer induces the formation of an azotobacter biofilm through a flavonoid compound, promotes the colonization and nitrogen fixation of rhizosphere azotobacter, improves the nitrogen supply capability of soil, and reduces the dependence on chemical nitrogen fertilizer. The fertilizer comprises the following components: 5-15% of a flavone extract, 3-8% of an azotobacter, 20-30% of an organic carrier, 30-40% of an inorganic nutrient medium and 5-15% of a filler. The preparation method comprises the following steps: mixing the organic carrier and the inorganic nutrient substrate, adding the filling agent, granulating, adding the nitrogen-fixing bacterial agent and the flavone extract to form a composite liquid, spraying the composite liquid, drying and packaging. Compared with the prior art, the fertilizer disclosed by the invention has the advantages of remarkably improving the nitrogen fixation efficiency, reducing the use of chemical nitrogen fertilizer, enhancing the slow release performance, improving the fertilizer utilization rate, improving the soil ecological environment and promoting the healthy growth of plants. The method is simple, efficient and suitable for large-scale production and application.

Description

Technical Field

[0001] The present invention relates to a flavonoid-induced microbial synergistic nitrogen-fixing fertilizer and a preparation method thereof, and belongs to the technical field of agricultural fertilizers. The fertilizer induces the biofilm formation of nitrogen-fixing bacteria through flavonoid compounds, promotes the colonization and nitrogen fixation of rhizosphere nitrogen-fixing bacteria, thereby improving the nitrogen supply capacity of the soil and reducing the dependence on chemical nitrogen fertilizers. Background Art

[0002] With the continuous advancement of the concept of sustainable agricultural development, the demand for efficient and environmentally friendly fertilizers is growing. Although traditional chemical nitrogen fertilizers can provide the necessary nitrogen for plant growth, their long-term and large-scale use will lead to a series of problems such as soil acidification and environmental pollution.

[0003] Flavonoids are a class of natural compounds widely found in plants and have multiple biological activities. Recent studies have shown that flavonoids can induce the formation of nitrogen-fixing bacterial biofilms in the soil, thereby promoting the colonization and nitrogen fixation of rhizosphere nitrogen-fixing bacteria. However, most of the flavonoid fertilizers currently available on the market focus on the extraction and application of flavonoids from a single plant source, and there are relatively few in-depth studies on the synergistic effects of multiple flavonoids and their combination with microorganisms.

[0004] Under the background of green agricultural development, environmentally friendly fertilizers have become an important material basis for the green development of agriculture. New fertilizers, such as slow / controlled release fertilizers, organic-inorganic compound fertilizers, microbial fertilizers, etc., have become the key direction for the development of environmentally friendly fertilizers. The research and development and promotion of these new fertilizers play an important role in improving fertilizer utilization, reducing environmental pollution, and promoting sustainable agricultural development.

[0005] Therefore, the development of a flavonoid-induced microbial synergistic nitrogen-fixing fertilizer can not only effectively utilize biological nitrogen fixation and reduce the use of chemical nitrogen fertilizers, but also improve the slow-release performance and utilization rate of fertilizers through the synergistic effect of various flavonoid compounds and microorganisms, while improving the soil ecological environment and promoting healthy plant growth. Summary of the invention

[0006] The purpose of the present invention is to provide a flavonoid-induced microbial synergistic nitrogen-fixing fertilizer and a preparation method thereof. The fertilizer enhances the nitrogen-fixing capacity of the soil, improves the nitrogen absorption efficiency of plants, and reduces the use of chemical nitrogen fertilizers by compounding a variety of flavonoid compounds with specific microbial strains.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] The first aspect of the present invention is to provide a flavonoid-induced microbial synergistic nitrogen-fixing fertilizer, comprising the following components: 5%-15% flavonoid extract, 3%-8% nitrogen-fixing bacteria, 20%-30% organic carrier, 30%-40% inorganic nutrient matrix, and 5%-15% filler.

[0009] As a further technical solution, in the flavonoid extract, the ratio of sweet potato flavonoid extract, soybean flavonoid extract and tea flavonoid extract is 5:3:2.

[0010] As a further technical solution, in the nitrogen-fixing bacteria agent, the ratio of rhizosphere nitrogen-fixing bacteria to leguminous rhizobia is 1:1 to 3:1. Wherein: the rhizosphere nitrogen-fixing bacteria is selected from Pseudomonas stutzeri, Azotobacter chroococcum or Azospirillum spp.; the leguminous rhizobia is selected from Rhizobium, Sinorhizobium or Bradyrhizobium.

[0011] As a further technical solution, in the organic carrier, the ratio of humic acid to biochar is 1:1 to 2:1.

[0012] As a further technical solution, in the inorganic nutrient matrix, the ratio of diammonium phosphate to potassium sulfate is 1:1 to 2:1.

[0013] As a further technical solution, the filler is a mixture of diatomaceous earth and bentonite, wherein the ratio of diatomaceous earth to bentonite is 1:1 to 2:1.

[0014] The second aspect of the present invention is to provide a method for preparing a flavonoid-induced microbial synergistic nitrogen-fixing fertilizer, comprising the following steps:

[0015] (1) adding 20%-30% of an organic carrier and 30%-40% of an inorganic nutrient matrix into a blender and mixing at a low speed (100-200 rpm) for 5 minutes;

[0016] (2) Add 5%-15% filler into a blender and mix at low speed (100-200 rpm) for 5 minutes;

[0017] (3) Increase the mixing speed to medium (300-500 rpm) and continue mixing for 5 minutes to ensure uniformity;

[0018] (4) The uniformly mixed material is granulated by a granulator to control the particle size between 2-5 mm;

[0019] (5) adding 3%-8% nitrogen-fixing bacteria agent and 5%-15% flavonoid extract into a blender in proportion, and mixing at a low speed (100-200 rpm) for 5 minutes to form a flavonoid-microorganism composite solution;

[0020] (6) spraying the flavonoid-microorganism composite liquid evenly on the surface of the material particles through a spraying device, and the spraying amount is 10%-20% of the weight of the material particles;

[0021] (7) After spraying, the material particles are dried at a temperature of 30-50°C for 12-24 hours;

[0022] (8) The dried material particles are packaged to obtain a flavonoid-induced microbial synergistic nitrogen-fixing fertilizer.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. Improve nitrogen fixation efficiency and reduce dependence on chemical nitrogen fertilizers: The present invention induces nitrogen-fixing bacteria to form biofilms through flavonoid compounds, promotes the colonization and nitrogen fixation of rhizosphere nitrogen-fixing bacteria, and thus significantly improves the nitrogen fixation capacity of the soil. Compared with traditional chemical nitrogen fertilizers, the present invention can reduce the use of chemical nitrogen fertilizers while meeting the nitrogen demand of plant growth, reducing agricultural production costs, and reducing environmental problems such as soil acidification and water eutrophication caused by excessive use of chemical nitrogen fertilizers.

[0025] 2. Enhance the slow-release performance of fertilizers and improve fertilizer utilization: The present invention adopts a compound of organic carriers (humic acid and biochar) and fillers (diatomaceous earth and bentonite), which can effectively adsorb and slowly release flavonoids and microorganisms, and prolong the effective action time of fertilizers. This slow-release property allows the nutrients in the fertilizer to be released more evenly, better meeting the needs of plants at different growth stages, thereby improving the utilization rate of fertilizers.

[0026] 3. Improve soil ecological environment and promote healthy plant growth: The nitrogen-fixing bacteria and flavonoid extracts in the present invention are both biologically active ingredients, which have a good promoting effect on the soil ecological environment. The nitrogen-fixing bacteria can increase the number and activity of beneficial microorganisms in the soil and improve the soil microecological environment; the flavonoid extract has multiple biological activities such as antioxidant and antibacterial activities, which can enhance the stress resistance of plants and promote the healthy growth of plants. DETAILED DESCRIPTION

[0027] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0028] In Example 1, a flavonoid-induced microbial synergistic nitrogen-fixing fertilizer includes the following components: 100 kg of flavonoid extract, 50 kg of nitrogen-fixing bacteria, 250 kg of organic carrier, 350 kg of inorganic nutrient matrix, and 100 kg of filler. The flavonoid extract includes 50 kg of sweet potato flavonoid extract, 30 kg of soybean flavonoid extract, and 20 kg of tea flavonoid extract. The nitrogen-fixing bacteria include 25 kg of rhizosphere nitrogen-fixing bacteria and 25 kg of leguminous plant rhizobia. The organic carrier includes 150 kg of humic acid and 100 kg of biochar. The inorganic nutrient matrix includes 175 kg of diammonium phosphate and 175 kg of potassium sulfate. The filler includes 50 kg of diatomaceous earth and 50 kg of bentonite.

[0029] Based on the above-mentioned flavonoid-induced microbial synergistic nitrogen-fixing fertilizer, a preparation method of the flavonoid-induced microbial synergistic nitrogen-fixing fertilizer is proposed: 250kg of organic carrier (150kg of humic acid and 100kg of biochar) and 350kg of inorganic nutrient matrix (175kg of diammonium phosphate and 175kg of potassium sulfate) are added to a blender and mixed at a low speed (100-200 rpm) for 5 minutes; 100kg of filler (50kg of diatomaceous earth and 50kg of bentonite) is added to the blender and mixed at a low speed (100-200 rpm) for 5 minutes; the mixing speed is increased to a medium speed (300-500 rpm), and the mixing is continued for 5 minutes to ensure uniformity; the uniformly mixed materials are passed through a sieve; The method comprises the following steps: granulating the material with a granulator to control the particle size between 2 and 5 mm; adding 50 kg of nitrogen-fixing bacteria (25 kg of rhizosphere nitrogen-fixing bacteria and 25 kg of leguminous plant rhizobia) and 100 kg of flavonoid extract (50 kg of sweet potato flavonoid extract, 30 kg of soybean flavonoid extract and 20 kg of tea flavonoid extract) into a blender, mixing at a low speed (100-200 rpm) for 5 minutes to form a flavonoid-microorganism composite liquid; spraying the flavonoid-microorganism composite liquid uniformly on the surface of material particles through a spraying device, and the spraying amount is 15% of the weight of the material particles; after spraying, drying the material particles at a temperature of 35° C. for 18 hours; and packaging the dried material particles to obtain a microbial synergistic nitrogen-fixing fertilizer based on flavonoid induction.

[0030] In Example 2, a flavonoid-induced microbial synergistic nitrogen-fixing fertilizer includes the following components: 150 kg of flavonoid extract, 50 kg of nitrogen-fixing bacteria, 200 kg of organic carrier, 300 kg of inorganic nutrient matrix, and 100 kg of filler. The flavonoid extract includes 75 kg of sweet potato flavonoid extract, 45 kg of soybean flavonoid extract, and 30 kg of tea flavonoid extract. The nitrogen-fixing bacteria include 25 kg of rhizosphere nitrogen-fixing bacteria and 25 kg of leguminous plant rhizobia. The organic carrier includes 120 kg of humic acid and 80 kg of biochar. The inorganic nutrient matrix includes 150 kg of diammonium phosphate and 150 kg of potassium sulfate. The filler includes 50 kg of diatomaceous earth and 50 kg of bentonite.

[0031] Based on the above-mentioned flavonoid-induced microbial synergistic nitrogen-fixing fertilizer, a preparation method of the flavonoid-induced microbial synergistic nitrogen-fixing fertilizer is proposed: 200kg of organic carrier (120kg of humic acid and 80kg of biochar) and 300kg of inorganic nutrient matrix (150kg of diammonium phosphate and 150kg of potassium sulfate) are added to a blender and mixed at a low speed (100-200 rpm) for 5 minutes; 100kg of filler (50kg of diatomaceous earth and 50kg of bentonite) is added to the blender and mixed at a low speed (100-200 rpm) for 5 minutes; the mixing speed is increased to a medium speed (300-500 rpm), and the mixing is continued for 5 minutes to ensure uniformity; the uniformly mixed material is passed through a A granulator is used for granulation, and a particle size is controlled between 2 and 5 mm; 50 kg of nitrogen-fixing bacteria (25 kg of rhizosphere nitrogen-fixing bacteria and 25 kg of leguminous plant rhizobia) and 150 kg of flavonoid extract (75 kg of sweet potato flavonoid extract, 45 kg of soybean flavonoid extract, and 30 kg of tea flavonoid extract) are added into a mixer, and mixed at a low speed (100-200 rpm) for 5 minutes to form a flavonoid-microorganism composite liquid; the flavonoid-microorganism composite liquid is evenly sprayed on the surface of material particles through a spraying device, and the spraying amount is 15% of the weight of the material particles; after spraying, the material particles are dried at a temperature of 50°C for 12 hours; the dried material particles are packaged to obtain a microbial synergistic nitrogen-fixing fertilizer based on flavonoid induction.

[0032] In Example 3, a flavonoid-induced microbial synergistic nitrogen-fixing fertilizer includes the following components: 50 kg of flavonoid extract, 80 kg of nitrogen-fixing bacteria, 300 kg of organic carrier, 320 kg of inorganic nutrient matrix, and 100 kg of filler. The flavonoid extract includes 25 kg of sweet potato flavonoid extract, 15 kg of soybean flavonoid extract, and 10 kg of tea flavonoid extract. The nitrogen-fixing bacteria include 40 kg of rhizosphere nitrogen-fixing bacteria and 40 kg of leguminous plant rhizobia. The organic carrier includes 180 kg of humic acid and 120 kg of biochar. The inorganic nutrient matrix includes 160 kg of diammonium phosphate and 160 kg of potassium sulfate. The filler includes 50 kg of diatomaceous earth and 50 kg of bentonite.

[0033] Based on the above-mentioned flavonoid-induced microbial synergistic nitrogen-fixing fertilizer, a preparation method of the flavonoid-induced microbial synergistic nitrogen-fixing fertilizer is proposed: 300 kg of organic carrier (180 kg of humic acid and 120 kg of biochar) and 320 kg of inorganic nutrient matrix (160 kg of diammonium phosphate and 160 kg of potassium sulfate) are added to a blender and mixed at a low speed (100-200 rpm) for 5 minutes; 100 kg of filler (50 kg of diatomaceous earth and 50 kg of bentonite) are added to the blender and mixed at a low speed (100-200 rpm) for 5 minutes; the mixing speed is increased to a medium speed (300-500 rpm), and the mixing is continued for 5 minutes to ensure uniformity; the uniformly mixed materials are passed through a sieve; The method comprises the following steps: granulating the material with a granulator to control the particle size between 2 and 5 mm; adding 80 kg of nitrogen-fixing bacteria (40 kg of rhizosphere nitrogen-fixing bacteria and 40 kg of leguminous plant rhizobia) and 50 kg of flavonoid extract (25 kg of sweet potato flavonoid extract, 15 kg of soybean flavonoid extract and 10 kg of tea flavonoid extract) into a blender, mixing at a low speed (100 to 200 revolutions per minute) for 5 minutes to form a flavonoid-microorganism composite liquid; spraying the flavonoid-microorganism composite liquid uniformly on the surface of material particles through a spraying device, with the spraying amount being 15% of the weight of the material particles; after spraying, drying the material particles at a temperature of 30° C. for 24 hours; and packaging the dried material particles to obtain a flavonoid-induced microbial synergistic nitrogen-fixing fertilizer.

[0034] The above content is a further detailed description of the present invention in combination with specific implementation methods. It cannot be considered that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, simple modifications and substitutions made without departing from the concept of the present invention should be deemed to belong to the scope of protection of the present invention.

Claims

1. A flavonoid-induced microbial synergistic nitrogen-fixing fertilizer, characterized in that: The invention comprises the following components: 5%-15% of flavonoid extract, 3%-8% of nitrogen-fixing bacteria agent, 20%-30% of organic carrier, 30%-40% of inorganic nutrient matrix and 5%-15% of filler.

2. The flavonoid-induced microbial synergistic nitrogen-fixing fertilizer according to claim 1, characterized in that: In the flavonoid extract, the ratio of the sweet potato flavonoid extract, the soybean flavonoid extract and the tea flavonoid extract is 5:3:

2.

3. The flavonoid-induced microbial synergistic nitrogen-fixing fertilizer according to claim 1, characterized in that: In the nitrogen-fixing bacteria agent, the ratio of rhizosphere nitrogen-fixing bacteria to leguminous plant rhizobia is 1:1 to 3:

1. Wherein: the rhizosphere nitrogen-fixing bacteria is selected from Pseudomonas stutzeri, Azotobacter chroococcum or Azospirillum spp.; the leguminous plant rhizobia is selected from Rhizobium, Sinorhizobium or Bradyrhizobium.

4. The flavonoid-induced microbial synergistic nitrogen-fixing fertilizer according to claim 1, characterized in that: In the organic carrier, the ratio of humic acid to biochar is 1:1 to 2:

1.

5. The flavonoid-induced microbial synergistic nitrogen-fixing fertilizer according to claim 1, characterized in that: In the inorganic nutrient matrix, the ratio of diammonium phosphate to potassium sulfate is 1:1 to 2:

1.

6. The flavonoid-induced microbial synergistic nitrogen-fixing fertilizer according to claim 1, characterized in that: The filler is a mixture of diatomaceous earth and bentonite, wherein the ratio of diatomaceous earth to bentonite is 1:1 to 2:

1.

7. A method for preparing a flavonoid-induced microbial synergistic nitrogen-fixing fertilizer according to claims 1 to 6, characterized in that: The following steps are involved: (1) adding 20%-30% of an organic carrier and 30%-40% of an inorganic nutrient matrix into a blender and mixing at a low speed (100-200 rpm) for 5 minutes; (2) Add 5%-15% filler into a blender and mix at low speed (100-200 rpm) for 5 minutes; (3) Increase the mixing speed to medium (300-500 rpm) and continue mixing for 5 minutes to ensure uniformity; (4) The uniformly mixed material is granulated by a granulator to control the particle size between 2-5 mm; (5) adding 3%-8% nitrogen-fixing bacteria agent and 5%-15% flavonoid extract into a blender in proportion, and mixing at a low speed (100-200 rpm) for 5 minutes to form a flavonoid-microorganism composite solution; (6) spraying the flavonoid-microorganism composite liquid evenly on the surface of the material particles through a spraying device, and the spraying amount is 10%-20% of the weight of the material particles; (7) After spraying, the material particles are dried at a temperature of 30-50°C for 12-24 hours; (8) The dried material particles are packaged to obtain a flavonoid-induced microbial synergistic nitrogen-fixing fertilizer.