Preparation method of rubber whey small molecule peptide-containing liquid fertilizer

Abstract

The invention relates to the technical field of preparation of liquid fertilizers, and discloses a preparation method of a liquid fertilizer containing rubber whey small molecule peptides, the materials comprise 50%-85% of rubber whey small molecule peptides and 15%-50% of other fertilizer components, the materials are weighed and stirred uniformly, and the other fertilizer components comprise macroelements, medium trace elements, amino acids, microbial agents and humic acid. The preparation method comprises the following steps: adding special screened lactic acid bacteria R22 fermentation liquor into natural rubber skim liquor, uniformly stirring, standing for 6-8 hours, taking out the rubber after the natural rubber is completely separated out, adding saccharomycetes FD6 into the residual skim liquor, fermenting for 24 hours, adding beta-1, 3-glucanase, breaking the wall of the saccharomycetes, adding bacillus velezensis GD13 fermentation liquor, and fermenting for 24 hours to obtain the natural rubber skim liquor. The preparation method comprises the following steps: decomposing rubber protein and yeast protein in the rubber whey to prepare a rubber whey small molecule peptide solution, and adding nitrogen phosphorus potassium or medium trace elements into the prepared rubber whey small molecule peptide solution to prepare the liquid fertilizer, so that the soil is effectively improved, and the yield and quality of crops are improved.

Description

Technical Field

[0001] The invention relates to the technical field of liquid fertilizer preparation, in particular to a method for preparing liquid fertilizer containing rubber whey small molecule peptides. Background Art

[0002] The main components of natural latex collected from the Brazilian rubber tree are rubber hydrocarbons, inositols, sugars, proteins, amino acids, nitrogenous bases, nucleic acids, organic acids and inorganic salts. At present, the gel of natural rubber mainly uses inorganic acid gelation, which is highly polluting and difficult to treat the subsequent wastewater. The latex contains a large amount of organic nutrients, especially high content of protein, alkaloids and white quebracho alcohol, which has a good effect on preventing agricultural diseases and promoting crop growth.

[0003] The present invention is a rubber making process using a biological method. Compared with the traditional acid method, the biological method can retain the organic active ingredients in the natural rubber latex to the maximum extent. At the same time, the use of highly active strains further improves the nutritional value of the entire fermentation liquid and enhances the biological stimulation function. The rubber clear obtained in this way has high nutrient content and rich organic matter, and is a very good raw material for organic fertilizer.

[0004] Peptides are compound fragments between amino acids and proteins. They are composed of two or more amino acid molecules connected by peptide bonds. They are structural and functional fragments of proteins. Many active substances in the body, such as hormones and enzymes, are essentially peptides. Peptides have strong biological activity, among which small molecule peptides composed of 2-6 amino acids are the most active.

[0005] A method for preparing a liquid fertilizer containing small molecule peptides from rubber whey is now invented. Through modern biotechnology, the nutrients in natural rubber whey are fully utilized, and then combined with microorganisms and other nutrients, so as to improve the soil, prevent soil-borne diseases, and promote crop growth. Summary of the invention

[0006] 1. Technical issues to be solved

[0007] In view of the deficiencies in the prior art, the present invention provides a method for preparing a liquid fertilizer containing rubber whey small molecule peptides, which solves the above-mentioned problems.

[0008] (II) Technical solution

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] Preferably, the rubber whey small molecule peptide, as a high-quality organic nutrition, not only has the functions of peptides or amino acids, but also has some functions of rubber whey liquid, such as disease prevention and stress resistance.

[0011] Preferably, when the rubber whey small molecule peptide is mixed and stirred with other fertilizer components, it is necessary to stir them evenly to ensure that they are fully mixed.

[0012] Preferably, macro-element type, trace element type and microbial agent type are respectively mixed with rubber whey small molecule peptide to prepare different liquid fertilizers.

[0013] Another technical problem to be solved by the present invention is to provide a preparation method of liquid fertilizer containing rubber whey small molecule peptides. The materials are 50% to 85% of rubber whey small molecule peptides and 15% to 50% of other fertilizer components. After weighing, each material is stirred evenly, tested and qualified, and then made into a product. The other fertilizer components include macroelements, medium and trace elements, amino acids, microbial agents and humic acid.

[0014] The rubber whey small molecule peptide is prepared as follows:

[0015] 1) Preparation of lactic acid bacteria fermentation liquid: fermenting the screened lactic acid bacteria R22 to a pH value of 3.8 to 4.2 for later use;

[0016] 2) Preparation of protease fermentation liquid: Prepare a culture medium for Bacillus Velez GD13 according to the fermentation process requirements, sterilize and ferment, and stop fermentation when the protease activity reaches 1500 U / ml;

[0017] 3) Mix the natural rubber latex and lactic acid bacteria fermentation liquid in a flocculation tank at a ratio of 1:1 and let it stand for 6-8 hours. After the rubber is completely solidified, extract the rubber and leave the remaining rubber whey extract;

[0018] 4) The rubber whey extract was pumped back into the fermentation tank, trehalose was added according to 0.5% of the fermentation amount, and after sterilization, yeast FD6 was added and fermented for 24 hours until the wet weight of the yeast reached more than 80 g / L;

[0019] 5) After yeast fermentation is completed, the fermentation liquid temperature is raised to 45°C, and β-1,3-glucanase is added to break the yeast cell wall to release yeast protein and yeast contents; the reaction is carried out for 48 hours;

[0020] 6) adding protease fermentation liquid, reacting for 20 to 24 hours, and stopping when the content of peptides with molecular weight between 300 and 1000 accounts for about 80%, thereby obtaining rubber whey small molecule peptides;

[0021] 7) The preparation ratio of the macro-element type, taking 100kg as an example: 60kg of rubber whey small molecule peptide, 25kg of urea, 10kg of potassium dihydrogen phosphate, and 5kg of potassium sulfate;

[0022] 8) The preparation ratio of trace element type, taking 100kg as an example: 85kg of rubber whey small molecule peptide, 7.5kg of zinc sulfate, 2.5kg of boric acid, 2.5kg of manganese sulfate, and 2.5kg of ferrous sulfate;

[0023] 9) Preparation ratio of microbial formulation, taking 100kg as an example: 85kg of rubber whey small molecule peptide and 15kg of 10 billion / ml Bacillus subtilis fermentation liquid.

[0024] (III) Beneficial effects

[0025] Compared with the prior art, the present invention provides a method for preparing liquid fertilizer containing rubber whey small molecule peptides, which has the following beneficial effects:

[0026] 1. The preparation method of the liquid fertilizer containing rubber whey small molecule peptides, adds specially selected microorganisms to the production waste of the rubber making process, uses modern biotechnology to turn waste into treasure, solves environmental protection problems, and also provides technical support for sustainable agricultural development. The organic nutrition in the rubber whey is enriched to cultivate rich yeast protein. The yeast protein is enzymatically hydrolyzed and the degree of enzymatic hydrolysis is controlled so that free amino acids account for about 10% and small peptides with a molecular weight of 300-1000 account for more than 80%. The organic waste liquid in the rubber process is utilized as a resource, which reduces environmental pressure on the one hand and increases the income of the rubber processing plant on the other hand.

[0027] 2. The preparation method of the liquid fertilizer containing rubber whey small molecule peptides has an obvious synergistic effect by using it in combination with macroelements, medium and trace elements, humic acid fertilizers, microbial agents, etc. The use of microbial agents cultivated by special processes has a qualitative improvement in its growth-promoting ability, providing a good product for wheat growth promotion.

[0028] 3. The preparation method of the liquid fertilizer containing rubber whey small molecule peptide can promote the elongation growth of plants, promote the growth of rhizomes and buds, make seedlings and growing plants grow rapidly to form strong seedlings and plants, increase the weight of leaves and stems, and promote the reduction rate of chlorophyll, protein and protoplasm content, thereby extending the life cycle of leaves. The peptide has strong chemical stability and can be mixed with various acidic or alkaline (PH = 4-9) fertilizers and pesticides, overcoming the shortcomings of poor miscibility and antagonism of traditional chemical regulators. The peptide is suitable for the entire growth period of all plants and can replace various traditional Chemical regulators (such as sodium complex, sodium naphthalene, etc.) can show obvious effects in 3-4 days. They are ideal foliar sprays and fertilizer additives for grade A and AA green foods. Peptides can make crop leaves thick and green, and petioles thick and emerald green; plants bloom early, bear fruit early, and go to market early, with beautiful fruit shape, pure flavor, good taste, and improved commercial properties. Peptides are widely used in crops and can significantly increase the growth rate and quality of crops. At the same time, they have good environmental performance. Rubber whey small molecule peptide liquid can add mineral nutrients such as nitrogen, phosphorus, potassium, calcium, magnesium, zinc, and iron according to the different crops and soil environments to enhance product effects.

[0029] 4. The preparation method of the liquid fertilizer containing rubber whey small molecule peptides, natural rubber skim milk has a good bactericidal function, and the use of skim milk for microbial culture is the key point. The present invention has obtained several strains of the present invention through multiple screenings, and the lactic acid bacteria are used to produce lactic acid, which can make the natural rubber hydrocarbon flocculate and precipitate on the one hand, and react with the alkaloids in the skim milk on the other hand. With the addition of trehalose, it can well ensure that the yeast can be fermented stably, improve the rhizosphere microenvironment of crops, promote the growth of the root system, improve nutrient absorption, reduce the use of chemical fertilizers, and effectively improve the utilization rate of fertilizers, without pollution and residue. DETAILED DESCRIPTION

[0030] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Embodiment 1:

[0032] A preparation method of liquid fertilizer containing rubber whey small molecule peptide, characterized in that: the material is 50% to 85% of rubber whey small molecule peptide, and other fertilizer components are 15% to 50%. After each material is weighed, it is stirred evenly, tested and qualified, and the product is made. The other fertilizer components include macroelements, medium and trace elements, amino acids, microbial agents and humic acid.

[0033] The rubber whey small molecule peptide is prepared as follows:

[0034] 1) Preparation of lactic acid bacteria fermentation liquid: fermenting the screened lactic acid bacteria R22 to a pH value of 3.8 to 4.2 for later use;

[0035] 2) Preparation of protease fermentation liquid: Prepare a culture medium for Bacillus Velez GD13 according to the fermentation process requirements, sterilize and ferment, and stop fermentation when the protease activity reaches 1500 U / ml;

[0036] 3) Mix the natural rubber latex and lactic acid bacteria fermentation liquid in a flocculation tank at a ratio of 1:1 and let it stand for 6-8 hours. After the rubber is completely solidified, extract the rubber and leave the remaining rubber whey extract;

[0037] 4) The rubber whey extract was pumped back into the fermentation tank, trehalose was added according to 0.5% of the fermentation amount, and after sterilization, yeast FD6 was added and fermented for 24 hours until the wet weight of the yeast reached more than 80 g / L;

[0038] 5) After yeast fermentation is completed, the fermentation liquid temperature is raised to 45°C, and β-1,3-glucanase is added to break the yeast cell wall to release yeast protein and yeast contents; the reaction is carried out for 48 hours;

[0039] 6) adding protease fermentation liquid, reacting for 20 to 24 hours, and stopping when the content of peptides with molecular weight between 300 and 1000 accounts for about 80%, thereby obtaining rubber whey small molecule peptides;

[0040] 7) The preparation ratio of the macro-element type, taking 100kg as an example: 60kg of rubber whey small molecule peptide, 25kg of urea, 10kg of potassium dihydrogen phosphate, and 5kg of potassium sulfate;

[0041] The present invention was compared with water-soluble fertilizer. Five days after transplanting tomatoes, the area was 0.5 mu each, and there were 1,000 seedlings. The present invention was 10 kg / mu and drip irrigation was used. The control water-soluble fertilizer 25-10-15, 10 kg / mu, drip irrigation, was used for 10 days, and the results were as follows: the disease prevention effect of the present invention: 17 seedlings died, and the seedling death rate was 1.7%; the growth promotion effect: an average of 2.4 new leaves, thick green and wide leaves, thick stems, and developed root systems; the control disease prevention effect was 57 seedlings dead, and the seedling death rate was 5.7%; the growth promotion effect: an average of 1.8 new leaves, thick green leaves, relatively small leaf surface, weak stems, and general root systems. Conventional management: 6542 jin per mu, no growth rate; the present invention + conventional management: 8339 jin per mu, and an increase rate of 27.46%. After using the present invention, the tomato seedling death rate decreased significantly, and the overall growth of the seedlings was better than the control. When the amount of nitrogen, phosphorus and potassium is less, the effect is better, the amount of fertilizer used is reduced, and it is more friendly to the soil and environment. After using the invention, the yield is increased by 27.46% compared with the control, and the yield increase effect is significant.

[0042] Embodiment 2:

[0043] A preparation method of liquid fertilizer containing rubber whey small molecule peptide, characterized in that: the material is 50% to 85% of rubber whey small molecule peptide, and other fertilizer components are 15% to 50%. After each material is weighed, it is stirred evenly, tested and qualified, and the product is made. The other fertilizer components include macroelements, medium and trace elements, amino acids, microbial agents and humic acid.

[0044] The rubber whey small molecule peptide is prepared as follows:

[0045] 1) Preparation of lactic acid bacteria fermentation liquid: fermenting the screened lactic acid bacteria R22 to a pH value of 3.8 to 4.2 for later use;

[0046] 2) Preparation of protease fermentation liquid: Prepare a culture medium for Bacillus Velez GD13 according to the fermentation process requirements, sterilize and ferment, and stop fermentation when the protease activity reaches 1500 U / ml;

[0047] 3) Mix the natural rubber latex and lactic acid bacteria fermentation liquid in a flocculation tank at a ratio of 1:1 and let it stand for 6-8 hours. After the rubber is completely solidified, extract the rubber and leave the remaining rubber whey extract;

[0048] 4) The rubber whey extract was pumped back into the fermentation tank, trehalose was added according to 0.5% of the fermentation amount, and after sterilization, yeast FD6 was added and fermented for 24 hours until the wet weight of the yeast reached more than 80 g / L;

[0049] 5) After yeast fermentation is completed, the fermentation liquid temperature is raised to 45°C, and β-1,3-glucanase is added to break the yeast cell wall to release yeast protein and yeast contents; the reaction is carried out for 48 hours;

[0050] 6) adding protease fermentation liquid, reacting for 20 to 24 hours, and stopping when the content of peptides with molecular weight between 300 and 1000 accounts for about 80%, thereby obtaining rubber whey small molecule peptides;

[0051] 7) The preparation ratio of trace element type, taking 100kg as an example: 85kg of rubber whey small molecule peptide, 7.5kg of zinc sulfate, 2.5kg of boric acid, 2.5kg of manganese sulfate, and 2.5kg of ferrous sulfate.

[0052] The present invention is compared with a certain brand of amino acid trace element water-soluble fertilizer, and the crop cucumber is grown in the following method: starting from the first cucumber, the rubber whey small molecule peptide liquid fertilizer is diluted 800 times for foliar spraying, and it is used once every 20 days; the amino acid trace element water-soluble fertilizer is diluted 500 times for foliar spraying, and it is used once every 20 days. Other management fertilizers are consistent. The effects are as follows: the disease prevention effect of the present invention: the overall incidence rate is low, and pesticides are used for disease prevention 3 times in the whole cycle; the growth promotion effect: the leaves are thick, green and wide, the stems are thick, the cucumbers swell quickly, the appearance is good, the skin is beautiful, and the taste is good. Slow aging, pick two more batches of melons; control: the overall incidence rate is low, and pesticides are used for disease prevention 5 times in the whole cycle; the leaves are green, the stems are thick, the cucumbers swell slowly, the appearance is good, the skin is beautiful, and the taste is good. The yield of the present invention is 8650kg / 0.5 mu; the control yield is 6720kg / 0.5 mu; after using the present invention, cucumber diseases are significantly reduced, and the use of pesticides is reduced. The growth of cucumbers was better than that of the control, the vines aged slowly, two more batches of cucumbers were harvested than the control, and the yield increased by 28.7% over the control, with a significant yield increase effect.

[0053] Embodiment three:

[0054] A preparation method of liquid fertilizer containing rubber whey small molecule peptide, characterized in that: the material is 50% to 85% of rubber whey small molecule peptide, and other fertilizer components are 15% to 50%. After each material is weighed, it is stirred evenly, tested and qualified, and the product is made. The other fertilizer components include macroelements, medium and trace elements, amino acids, microbial agents and humic acid.

[0055] The rubber whey small molecule peptide is prepared as follows:

[0056] 1) Preparation of lactic acid bacteria fermentation liquid: fermenting the screened lactic acid bacteria R22 to a pH value of 3.8 to 4.2 for later use;

[0057] 2) Preparation of protease fermentation liquid: Prepare a culture medium for Bacillus Velez GD13 according to the fermentation process requirements, sterilize and ferment, and stop fermentation when the protease activity reaches 1500 U / ml;

[0058] 3) Mix the natural rubber latex and lactic acid bacteria fermentation liquid in a flocculation tank at a ratio of 1:1 and let it stand for 6-8 hours. After the rubber is completely solidified, extract the rubber and leave the remaining rubber whey extract;

[0059] 4) The rubber whey extract was pumped back into the fermentation tank, trehalose was added according to 0.5% of the fermentation amount, and after sterilization, yeast FD6 was added and fermented for 24 hours until the wet weight of the yeast reached more than 80 g / L;

[0060] 5) After yeast fermentation is completed, the fermentation liquid temperature is raised to 45°C, and β-1,3-glucanase is added to break the yeast cell wall to release yeast protein and yeast contents; the reaction is carried out for 48 hours;

[0061] 6) adding protease fermentation liquid, reacting for 20 to 24 hours, and stopping when the content of peptides with molecular weight between 300 and 1000 accounts for about 80%, thereby obtaining rubber whey small molecule peptides;

[0062] 7) Preparation ratio of microbial formulation, taking 100kg as an example: 85kg of rubber whey small molecule peptide and 15kg of 10 billion / ml Bacillus subtilis fermentation liquid.

[0063] The present invention was compared with 1 billion / ml Bacillus subtilis inoculant, and the crop was grapes, and the location was Lankao County, Kaifeng, Henan. The method is as follows: the present invention is 10kg / mu, drip irrigation; the control is 10kg / mu, drip irrigation; it is used from the budding period, and it is used three times continuously, with an interval of 20 days, and other water and fertilizer management is the same. The effect is as follows: the use effect of the present invention: the root system is well-developed, the soil is fluffy, the buds are neat and strong, the leaves are dark green after unfolding, and the flower buds are neat and strong; the use effect of the control: the soil is fluffy, the root system is not good, and some white roots are blackened and dead; the buds are uneven, and the growth is a little slow; the flower buds are neat; the yield of the present invention is 2945kg / mu; the yield of the control is 2435kg / mu; after using the present invention, the root growth, budding, sprouting, flower bud growth, etc. of the grapes are better than the control, and the plants are strong after use, and the yield is increased by 20.94% compared with the control, and the yield increase effect is significant.

[0064] The beneficial effects of the present invention are as follows: in the present invention, the production waste of the rubber making process is added with specially selected microorganisms, and modern biotechnology is used to turn waste into treasure, which solves environmental protection problems and provides technical support for sustainable agricultural development. The organic nutrition in rubber skim is enriched to cultivate rich yeast protein, and the degree of enzymolysis of yeast protein is controlled so that free amino acids account for about 10% and small peptides with a molecular weight of 300-1000 account for more than 80%. Through modern biotechnology, the nutrients in natural rubber whey are fully utilized, and then combined with microorganisms and other nutrients, so as to improve the soil, prevent soil-borne diseases, and promote crop growth. The resource utilization of organic waste liquid in the rubber process can reduce environmental pressure on the one hand and increase the income of the rubber processing plant on the other hand. Natural rubber skim has a good bactericidal function, and using skim to cultivate microorganisms is a difficult point. The present invention has obtained several strains of the present invention through multiple screenings. The present invention uses lactic acid bacteria to ferment and produce lactic acid, which can make natural rubber hydrocarbons flocculate and precipitate on the one hand, and can react with alkaloids in skim on the other hand. With the addition of trehalose, it can well ensure that yeast can ferment stably. After use, it can improve the root microenvironment of crops, promote root growth, increase nutrient absorption, reduce the use of chemical fertilizers, effectively improve the utilization rate of fertilizers, and is pollution-free and residue-free. The application of peptides on crops is mainly reflected in the following aspects:

[0065] 1. Promote growth: Peptides can promote the elongation growth of plants, promote the growth of roots, stems and buds, make seedlings and growing plants grow rapidly to form strong seedlings and plants, and increase the weight of leaves and stems.

[0066] 2. Prolong leaf senescence: Peptides can promote the reduction rate of chlorophyll, protein and protoplasm content, thereby extending the life cycle of leaves.

[0067] 3. Strong chemical stability: Peptides have strong chemical stability and can be mixed with various acidic or alkaline (PH = 4-9) fertilizers and pesticides, overcoming the shortcomings of poor miscibility and antagonism of traditional chemical regulators.

[0068] 4. Broad spectrum, high efficiency, green and environmentally friendly: Peptides are suitable for the entire growth period of all plants and can replace various traditional chemical regulators (such as sodium complex, sodium naphthalene, etc.). Obvious effects can be seen in 3-4 days. It is an ideal foliar spray and fertilizer additive for Class A and AA green foods.

[0069] 5. Improve crop quality: Peptides can make crop leaves thick and green, petioles thick and emerald green; plants bloom early, bear fruit early, go to market early, have beautiful fruit shape, pure flavor, good taste, and improve commercial quality.

[0070] Peptides are widely used in crops and can significantly improve the growth rate and quality of crops, while also having good environmental performance.

[0071] As a high-quality organic nutrition, rubber whey small molecule peptides not only have the functions of peptides or amino acids, but also have some functions of rubber whey liquid, such as disease prevention and stress resistance. Rubber whey small molecule peptide liquid can add mineral nutrients such as nitrogen, phosphorus, potassium or calcium, magnesium, zinc, iron, etc. according to the different crops and soil environments to improve the product effect. When the present invention is used, it is applied by flushing or drip irrigation at a dosage of 10kg per mu. The present invention is used in combination with macroelements, medium and trace elements, humic acid fertilizers, microbial agents, etc., and has obvious synergistic effects.

[0072] In summary, the ability of the fungal agent cultivated using special technology to promote growth has been qualitatively improved, providing a good product for promoting crop growth.

[0073] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a liquid fertilizer containing rubber whey small molecule peptides, characterized in that: The material is 50% to 85% of rubber whey small molecule peptide and 15% to 50% of other fertilizer components. After weighing each material, stir it evenly and make the product after passing the test. Other fertilizer components include macroelements, medium and trace elements, amino acids, microbial agents and humic acid. The rubber whey small molecule peptide is prepared as follows: 1) Preparation of lactic acid bacteria fermentation liquid: fermenting the screened lactic acid bacteria R22 to a pH value of 3.8 to 4.2 for later use; 2) Preparation of protease fermentation liquid: Prepare a culture medium for Bacillus Velez GD13 according to the fermentation process requirements, sterilize and ferment, and stop fermentation when the protease activity reaches 1500 U / ml; 3) Mix the natural rubber latex and lactic acid bacteria fermentation liquid in a flocculation tank at a ratio of 1:1 and let it stand for 6-8 hours. After the rubber is completely solidified, extract the rubber and leave the remaining rubber whey extract; 4) The rubber whey extract was pumped back into the fermentation tank, trehalose was added according to 0.5% of the fermentation amount, and after sterilization, yeast FD6 was added and fermented for 24 hours until the wet weight of the yeast reached more than 80 g / L; 5) After yeast fermentation is completed, the fermentation liquid temperature is raised to 45°C, and β-1,3-glucanase is added to break the yeast cell wall to release yeast protein and yeast contents; the reaction is carried out for 48 hours; 6) adding protease fermentation liquid, reacting for 20 to 24 hours, and stopping when the content of peptides with molecular weight between 300 and 1000 accounts for about 80%, thereby obtaining rubber whey small molecule peptides; 7) The preparation ratio of the macro-element type, taking 100kg as an example: 60kg of rubber whey small molecule peptide, 25kg of urea, 10kg of potassium dihydrogen phosphate, and 5kg of potassium sulfate; 8) The preparation ratio of trace element type, taking 100kg as an example: 85kg of rubber whey small molecule peptide, 7.5kg of zinc sulfate, 2.5kg of boric acid, 2.5kg of manganese sulfate, and 2.5kg of ferrous sulfate; 9) Preparation ratio of microbial formulation, taking 100kg as an example: 85kg of rubber whey small molecule peptide and 15kg of 10 billion / ml Bacillus subtilis fermentation liquid.

2. The method for preparing a liquid fertilizer containing rubber whey small molecule peptides according to claim 1, wherein: As a high-quality organic nutrition, rubber whey small molecule peptides not only have the functions of peptides or amino acids, but also have some functions of rubber whey, such as disease prevention and stress resistance.

3. The method for preparing a liquid fertilizer containing rubber whey small molecule peptides according to claim 1, characterized in that: When mixing rubber whey small molecule peptide with other fertilizer ingredients, it is necessary to stir evenly to ensure full mixing.

4. The method for preparing a liquid fertilizer containing rubber whey small molecule peptides according to claim 1, characterized in that: Macroelement type, trace element type and microbial agent type are respectively mixed with rubber whey small molecule peptide to prepare different liquid fertilizers.