Novel efficient compact immunofluorescence light path design scheme

Abstract

The invention provides a novel efficient compact immunofluorescence light path design scheme, and aims to solve the problems of large light path loss, complex structure and high cost in the prior art. The scheme comprises an LED light source, an excitation optical filter, a dichroscope, an aspherical lens and an emission optical filter, and finally data are received and collected by a photodiode. The components are sequentially arranged on the same optical axis, so that the light path is simplified, and the cost is reduced. By optimizing the light path structure, the fluorescence detection efficiency and the signal-to-noise ratio are improved, and meanwhile, the stray light suppression technology and software correction are adopted, so that the image contrast and definition are further improved. The design has the advantages of miniaturization, high efficiency, portability and simplicity and convenience in operation, is suitable for the technical field of in-vitro diagnosis, in particular to an immunofluorescence detection technology, and has wide application prospects and market competitiveness.

Description

1. Technical Field

[0001] The present invention relates to the technical field of in vitro diagnosis (IVD), immunofluorescence detection technology, and an immunofluorescence photoelectric signal detection device and method. 2. Background technology

[0002] With the development of biomedical detection technology, immunofluorescence detection technology has been widely used in the field of clinical detection and analysis due to its high sensitivity, high specificity and convenient operation. The core of immunofluorescence detection technology is to use a specific light source to excite the fluorescent substance in the antigen-antibody conjugate. The substance radiates fluorescence under the action of the excitation light, and the fluorescent signal is quantitatively detected and analyzed through the optical path system, thereby realizing accurate detection of the target. However, there are some limitations in the optical path design of existing immunofluorescence detection instruments. For example, some optical path mechanisms use optical dichroic mirrors to realize the input of light and the transmission of fluorescent signals, resulting in a large loss of the intensity of light and fluorescent signals during the detection process, thereby affecting the detection accuracy and sensitivity. In addition, some optical path mechanisms have complex structures and high manufacturing costs, which cannot meet the needs of low-cost and high-precision immunofluorescence detection.

[0003] In order to solve the above problems, the new immunofluorescence optical path design needs to simplify the optical path system and reduce costs while maintaining high sensitivity and high specificity. This includes optimizing the optical path structure to reduce the loss of light and fluorescent signals, improving detection accuracy and sensitivity, and also considering the miniaturization and integration of the optical path system to facilitate portability and operation and reduce manufacturing costs. Therefore, the development of a new, efficient and compact immunofluorescence optical path design is of great significance to improving the application scope and market competitiveness of immunofluorescence detection technology. 3. Summary of the invention

[0004] The present invention provides a novel, highly efficient and compact immunofluorescence optical path structure, comprising an LED light source, an excitation filter, a dichroic mirror, an aspheric lens and an emission filter. These components are arranged in sequence in an optical path component, wherein the LED light source constitutes an excitation optical path, the excitation filter, the dichroic mirror, the aspheric lens and the emission filter constitute a fluorescence detection optical path, and the excitation optical path and the fluorescence detection optical path are on the same optical axis.

[0005] LED light source: As an excitation light source, it emits light of a specific wavelength to excite fluorescent substances to emit light.

[0006] Excitation filter: Located between the LED light source and the dichroic mirror, it is used to filter out unnecessary wavelengths and only allow fluorescence signals of specific wavelengths to pass through, thereby improving the specificity of detection.

[0007] Dichroic mirror: It is used to separate the excitation light emitted by the laser from the fluorescence signal, ensuring that the excitation light can effectively illuminate the sample while reflecting the fluorescence signal to the detection path.

[0008] Aspherical lens: Located above the test strip, its focal point can fall on the detection area of ​​the test strip and is used to focus the fluorescent signal.

[0009] Emission filter: used to block unnecessary stray light, reduce background noise, and improve the signal-to-noise ratio of detection.

[0010] Photodiode: used to detect the fluorescence signal after focusing and filtering, and convert the optical signal into an electrical signal for subsequent signal processing and analysis.

[0011] Compared with the prior art, the beneficial effects of the present invention are:

[0012] Miniaturized design: The optical path structure of the present invention is compactly designed. Through the integrated optical module, multiple optical elements such as lenses, filters and light sources are integrated into a compact module, which simplifies the optical path design of the device and ensures efficient optical performance. At the same time, the compact design is adopted, and the multi-channel imaging system is integrated, including a compact three-dimensional structure and a photodiode acquisition module, which further reduces the size of the device.

[0013] High efficiency: The present invention improves the efficiency of fluorescence detection by optimizing the optical path structure design. For example, by using aspherical mirrors and low-dispersion materials to reduce the distortion of optical devices, optimize the lens arrangement and relative position, and avoid unnecessary light reflections. In addition, by using software correction to reduce the impact of stray light on imaging, the image contrast and clarity are further improved.

[0014] Stray light suppression: In order to improve the performance of the optical system, the present invention adopts a variety of stray light suppression methods, including aperture design, optical coating, surface treatment, lens design optimization, light path shielding structure, isolation and shielding, etc. These methods work together to effectively suppress stray light and improve signal acquisition quality.

[0015] Portability and ease of operation: The compact design of the present invention is not only easy to carry, but also easy to operate, and can be integrated into multi-channel equipment or other multi-functional equipment in the form of a module. 4. Description of the drawings

[0016] Figure 1 This is a new type of efficient and compact immunofluorescence optical path structure diagram.

[0017] Figure 2 It is the LED light source structure and package size.

[0018] Figure 3 It is the reflection and refraction of light of a specified wavelength by the excitation filter.

[0019] Figure 4 It is the reflection and refraction of light of a specified wavelength by a dichroic mirror.

[0020] Figure 5 This is the structure and size diagram of the aspheric lens.

[0021] Figure 6 It is the reflection and refraction of light of a specified wavelength by the emission filter. 5. Specific implementation methods

[0022] Component selection and layout: The novel, highly efficient and compact immunofluorescence optical path design in the embodiment of the present invention includes the following components: LED light source (1), excitation filter (2), dichroic mirror (3), aspheric lens (4) and emission filter (5). These components are arranged in sequence on the same optical axis to achieve efficient optical path transmission and detection.

[0023] Optical path structure design: In the present invention, the optical path structure design is compact, and multiple optical components are integrated into a compact module through an integrated optical module. The specific implementation is as follows:

[0024] The light from the light source (1) first passes through the excitation filter (2), and the filtered light is irradiated onto the dichroic mirror (3). The dichroic mirror (3) reflects the excitation light to the sample, while allowing the fluorescence signal to pass through and reflect to the aspheric lens (4). The aspheric lens (4) focuses the fluorescence signal and allows it to pass through the emission filter (5), further reducing stray light. The light signal after the emission filter (5) is received by the photodiode (not mentioned in the component list, but required for detecting the fluorescence signal) and converted into an electrical signal.

[0025] Stray light suppression: In order to improve the performance of the optical system, the present invention adopts a variety of stray light suppression methods, including aperture design, optical coating, surface treatment, lens design optimization, light path shielding structure, isolation and shielding, etc. These methods work together to effectively suppress stray light and improve signal acquisition quality.

[0026] Software correction: In order to further improve image contrast and clarity, the present invention also includes software correction technology, which reduces the impact of stray light on imaging through algorithm optimization and improves the accuracy of detection results.

Claims

1. A new efficient and compact immunofluorescence optical path design, characterized in that: The invention comprises the following components: an LED light source (1); an excitation filter (2); a dichroic mirror (3); an aspherical lens (4); and an emission filter (5); the above components are arranged in sequence on the same optical axis.

2. The novel, highly efficient and compact immunofluorescence optical path design according to claim 1, characterized in that: The LED light source (1) emits light of a specific wavelength to excite the fluorescent substance to emit light.

3. The novel, highly efficient and compact immunofluorescence optical path design according to claim 1 is characterized in that: The excitation filter (2) is located between the LED light source (1) and the dichroic mirror (3) and is used to filter out unnecessary wavelengths.

4. The novel, highly efficient and compact immunofluorescence optical path design according to claim 1, characterized in that: The dichroic mirror (3) is used to separate the excitation light from the fluorescence signal.

5. The novel, highly efficient and compact immunofluorescence optical path design according to claim 1, characterized in that: The aspherical lens (4) is located above the test strip, and its focus can fall on the detection area of ​​the test strip, so as to focus the fluorescent signal.

6. The novel, highly efficient and compact immunofluorescence optical path design according to claim 1, characterized in that: The emission filter (5) is used to block unnecessary stray light and reduce background noise.

7. The novel, highly efficient and compact immunofluorescence optical path design according to any one of claims 1 to 6, characterized in that: The optical path structure is compactly designed, and multiple optical elements are integrated into a compact module through an integrated optical module.

8. The novel, highly efficient and compact immunofluorescence optical path design according to any one of claims 1 to 7, characterized in that: The optical path structure is simple and occupies little space, and the reflection of the color separation beam splitter (3) converts the optical path from horizontal to vertical, further reducing the overall volume of the optical path structure.

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