Automatic sampling device and method for rapid starch detection

Abstract

The present invention relates to the field of starch detection and provides an automatic sampling device and method for rapid starch detection, comprising: a solution container for placing detection solutions at various stages of starch detection; a sensing device disposed in the solution container for acquiring liquid level information in the solution container; a control device comprising a reagent dropping device, a heating device, a stirring device, and a valve control device; and a control system for acquiring liquid level information transmitted by the sensing device and driving the control device according to a preset program to complete automated starch detection. The present invention adopts an automatic sampling and control system to replace manual operation, thereby reducing the size of the detection instrument and improving detection efficiency.

Description

Technical Field

[0001] The present invention relates to the field of starch detection, and in particular to an automatic sampling device and method for rapid starch detection. Background Art

[0002] The main source of sugar in the human body is starch in the diet. Testing the starch content can help people control the starch in their diet to achieve the goal of controlling sugar. The current national standard uses ultraviolet spectrophotometry to test starch content. Its pre-treatment is as follows: Figure 1 As shown, it is necessary to manually prepare the solution, perform heating, stirring, cooling, record the time, and perform corresponding solution addition and mixing operations at the specified time. The pre-treatment operation is complicated and time-consuming. Summary of the Invention

[0003] The problem solved by the present invention is how to provide an automatic sampling device for rapid starch detection which adopts an automatic sampling and control system to replace manual operation, can reduce the volume of the detection instrument and improve the detection efficiency.

[0004] In order to solve the above problems, the present invention provides an automatic sampling device for rapid starch detection, comprising: a solution container for placing detection solutions at various stages of starch detection;

[0005] A sensing device is provided in the solution container and is used to obtain liquid level information in the solution container;

[0006] Control equipment, including reagent adding equipment, heating equipment, stirring equipment and valve control equipment;

[0007] The control system is used to obtain the liquid level information transmitted by the sensing device and drive the control device according to a preset program to complete the automatic detection of starch.

[0008] Furthermore, the solution vessel includes a reaction tank, a detection tank, a waste liquid tank and a sand core filter funnel, and the valve control device includes a first solenoid valve and a second solenoid valve. The inlet of the reaction tank is used to add the starch to be detected, and the outlet channel of the reaction tank passes through the first solenoid valve and the sand core filter funnel in sequence to reach the inlet of the detection tank, and the outlet channel of the detection tank passes through the second solenoid valve to reach the inlet of the waste liquid tank.

[0009] Furthermore, the heating device includes a heating wire, which is used to heat the solution in the reaction tank. The stirring device includes a stirring rod, which is used to stir the solution in the reaction tank.

[0010] Furthermore, the reagent adding device includes a first peristaltic pump, a second peristaltic pump, a third peristaltic pump and a fourth peristaltic pump, and the first peristaltic pump and the second peristaltic pump are connected to the reaction tank;

[0011] The first peristaltic pump is driven by a control system to drop NaOH solution into the reaction tank, and then the control system controls the movement of the heating wire and the stirring rod to disperse the starch paste in the reaction tank;

[0012] The second peristaltic pump is driven by a control system to drop a mixed solution of n-butanol and isoamyl alcohol into the reaction tank, and then the mixture is heated, stirred and cooled to achieve separation of amylose and amylopectin.

[0013] Furthermore, the third peristaltic pump is connected to the detection cell and driven by the control system to drop iodine reagent into the detection cell to complete the color development detection of the solution in the detection cell;

[0014] The fourth peristaltic pump is connected to the sand core filter funnel. When the reaction is completed and the reaction pool solution flows through the sand core filter funnel, the amylose remains on the sand core filter screen of the sand core filter funnel. The fourth peristaltic pump is used to add formic acid into the sand core filter funnel to dissolve the amylose on the sand core filter screen.

[0015] Further, the sensing device includes a first liquid level sensor, a second liquid level sensor, a third liquid level sensor and a fourth liquid level sensor;

[0016] The first liquid level sensor and the second liquid level sensor are arranged one above the other in the reaction tank. During the reaction, the control system obtains first liquid level information after the addition of NaOH solution through the first liquid level sensor, and obtains second liquid level information after the addition of n-butanol-isoamyl alcohol mixed solution through the second liquid level sensor, thereby controlling the shutoff of the first peristaltic pump and the second peristaltic pump;

[0017] The third liquid level sensor and the fourth liquid level sensor are arranged in the detection pool. The third liquid level sensor obtains third liquid level information when the amylopectin solution enters the detection pool, and the fourth liquid level sensor obtains fourth liquid level information when the amylose enters the detection pool. The control system receives the third liquid level information and the fourth liquid level information and drives the third peristaltic pump to add a preset volume of iodine reagent to the detection pool.

[0018] Furthermore, the control system includes a power supply module, a main control chip, a first drive chip, a second drive chip, a liquid level sensing module, a button module, a heating module, a solenoid valve module, a stirring module and a reagent adding module.

[0019] Furthermore, the input end of the liquid level sensing module is respectively connected to the first liquid level sensor, the second liquid level sensor, the third liquid level sensor and the fourth liquid level sensor, and the output end is respectively connected to the four input ports of the main control chip. The button module includes a first button and a second button, and the first button and the second button are respectively connected to the input end of the main control chip.

[0020] Furthermore, the controlled ends of the first driver chip and the second driver chip are respectively connected to the output end of the main control chip, and the power end is connected to the power supply module. The output ends of the first driver chip are respectively connected to the heating module, the solenoid valve module and the stirring module. The heating module drives the heating wire, the solenoid valve module drives the first solenoid valve and the second solenoid valve, and the stirring module drives the stirring motor to drive the stirring rod; the four output ends of the second driver chip respectively drive the first peristaltic pump, the second peristaltic pump, the third peristaltic pump and the fourth peristaltic pump.

[0021] The present invention also provides a method for rapid starch detection and automatic sampling, based on the above-mentioned automatic starch detection and automatic sampling device, comprising:

[0022] Put soluble starch into the reaction tank, turn on the power, and give a start signal through the button module;

[0023] The first peristaltic pump motor rotates to add NaOH solution dropwise, and the stirring rod starts stirring;

[0024] When the first liquid level information shows that there is water, the first peristaltic pump motor stops rotating, and the heating wire turns off after heating for the set time;

[0025] Heating stops, and the second peristaltic pump motor rotates to add the n-butanol-isoamyl alcohol mixed solution dropwise until the first liquid level information and the second liquid level information both show water. The second peristaltic pump motor stops rotating, and the stirring rod and the heating wire turn off after working for the set time;

[0026] After the stirring rod and the heating wire are turned off, the first solenoid valve is opened, and the solution in the reaction pool enters the detection pool through the first solenoid valve and the sand core filter funnel;

[0027] Until the first liquid level information and the second liquid level information both show no water, and the third liquid level information shows water, the first solenoid valve is closed, and the third peristaltic pump motor rotates to add iodine reagent dropwise for 5 seconds and then stops, completing the pullulan solution test;

[0028] The main control system makes a judgment based on the information from the key module. If it receives a detection signal of amylose solution, it opens the second solenoid valve for a preset time to allow all the solution in the detection pool to flow into the waste liquid pool, and then closes the second solenoid valve.

[0029] The fourth peristaltic pump motor rotates to add formic acid solution until the fourth liquid level information shows that there is water. The fourth peristaltic pump motor stops rotating. The third peristaltic pump motor rotates to add iodine reagent for 5 seconds and then stops, completing the amylose solution detection.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] This equipment has low cost and small size, and realizes the artificial intelligence and automation of complex processing before starch detection. Compared with manual operation, it greatly improves the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is a schematic diagram of the existing manual starch pre-treatment process;

[0033] Figure 2 This is a schematic diagram of the structure of an automatic sampling device for rapid starch detection according to an embodiment of the present invention;

[0034] Figure 3 This is a schematic diagram of the circuit principle structure of a control system according to an embodiment of the present invention;

[0035] Figure 4 This is a schematic diagram of the automated starch pretreatment process for an embodiment of the present invention.

[0036] Description of reference numerals:

[0037] 1-reaction tank; 2-detection tank; 3-waste liquid tank; 4-sand core filter funnel; 5-stirring rod; 6-heating wire; 7-first solenoid valve; 8-second solenoid valve; 9-first liquid level sensor; 10-second liquid level sensor; 11-third liquid level sensor; 12-fourth liquid level sensor; 13-first peristaltic pump; 14-second peristaltic pump; 15-third peristaltic pump; 16-fourth peristaltic pump. DETAILED DESCRIPTION

[0038] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, specific embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0039] In the description of the present invention, it should be noted that, unless otherwise expressly specified or limited, the terms "disposed," "installed," "connected," and "connected" should be understood broadly. For example, they may refer to fixed, detachable, or integral connections; mechanical connections; direct connections or indirect connections via an intermediate medium; and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0040] Throughout this specification, references to the terms "embodiment," "one embodiment," and "an implementation" indicate that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or implementation are included in at least one embodiment or implementation of the present invention. In this specification, schematic representations of these terms do not necessarily refer to the same embodiment or implementation. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or implementations.

[0041] like Figure 2 As shown, the present invention provides an automatic sampling device for rapid starch detection, comprising: a solution container for placing detection solutions at various stages of starch detection;

[0042] A sensing device is provided in the solution container and is used to obtain liquid level information in the solution container;

[0043] Control equipment, including reagent adding equipment, heating equipment, stirring equipment and valve control equipment;

[0044] The control system is used to obtain the liquid level information transmitted by the sensing device and drive the control device according to a preset program to complete the automatic detection of starch.

[0045] It should be noted that if Figure 2 As shown, the solution vessel includes a reaction pool 1, a detection pool 2, a waste liquid pool 3 and a sand core filter funnel 4, the valve control device includes a first solenoid valve 7 and a second solenoid valve 8, the outlet channel of the reaction pool 1 passes through the first solenoid valve 7 and the sand core filter funnel 4 in sequence to reach the inlet of the detection pool 2, and the outlet channel of the detection pool 2 passes through the second solenoid valve 8 to reach the inlet of the waste liquid pool 3, the heating device includes an electric heating wire 6, the stirring device includes a stirring rod 5, and the stirring rod 5 is driven by a stirring motor, and the reagent dropping device includes a first peristaltic pump 13, a second peristaltic pump 14, The third peristaltic pump 15 and the fourth peristaltic pump 16, the first peristaltic pump 13 and the second peristaltic pump 14 are connected to the reaction tank 1, the third peristaltic pump 15 is connected to the detection tank 2, and the fourth peristaltic pump 16 is connected to the sand core filter funnel 4. The sensing device includes a first liquid level sensor 9, a second liquid level sensor 10, a third liquid level sensor 11 and a fourth liquid level sensor 12. The first liquid level sensor 9 and the second liquid level sensor 10 are arranged in the reaction tank 1, and the third liquid level sensor 11 and the fourth liquid level sensor 12 are arranged in the detection tank 2. The operating principle of the system is:

[0046] After starch is added to the reaction tank 1, the first peristaltic pump 13 drips the NaOH solution until it reaches the sensing electrode position of the first liquid level sensor 9, stops, and the stirring rod 5 rotates and the heating wire 6 heats to completely disperse the starch paste;

[0047] The second peristaltic pump 14 drips the n-butanol-isoamyl alcohol mixed solution and stops when the solution reaches the sensing electrode position of the second liquid level sensor 10. The stirring rod 5 rotates and the heating wire 6 heats. After cooling, the liquid is amylopectin and the solid is amylose.

[0048] The first solenoid valve 7 is opened, and the amylopectin solution is automatically injected into the detection cell 2 after filtering through the sand core, while the amylose remains on the sand core filter. The third peristaltic pump 15 adds iodine reagent for color development, and the test is then completed;

[0049] The second solenoid valve 8 is opened, and the pullulan solution flows into the waste liquid tank 3 after detection;

[0050] The fourth peristaltic pump 16 drips formic acid to dissolve the amylose solid on the sand core filter and flow into the detection tank 2. After detection, the second solenoid valve 8 opens and the amylose solution also flows into the waste liquid tank 3.

[0051] In one embodiment of the present invention, to achieve the above-mentioned starch detection, the control system is as follows Figure 3 As shown, it includes a power supply module, a main control chip, a first drive chip, a second drive chip, a liquid level sensing module, a button module, a heating module, a solenoid valve module, a stirring module and a reagent adding module.

[0052] It should be noted that the main control chip uses a single-chip microcomputer chip U2, whose input port is connected to 4 liquid level sensors and the first and second buttons, and the output end controls the heating wire 6, stirring motor, 2 solenoid valves and 4 peristaltic pump motors through the driver chips Q1 and Q2; with the STC89C52 single-chip microcomputer as the control core, the potential signal change caused by the liquid level sensor causes the high and low potential changes of the single-chip microcomputer control pin, thereby controlling the rotation of the peristaltic pump motor to realize the dripping of reagents, the rotation of the motor drives the glass cup to rotate for stirring, the opening and closing of the solenoid valve, and the heating wire 6 to achieve the purpose of automatic injection of amylopectin and amylose. After turning on the power, the initialization program is called, and the button detection program is called cyclically. When the first and second buttons are not pressed, the pins both output high levels, and the system does not change. When the first button is pressed, the system will automatically extract the amylopectin solution. After that, if amylose detection is required, pressing the second button will extract the amylose solution.

[0053] According to the above control system, the overall starch rapid detection automatic sampling method of the present invention is as follows Figure 4 As shown, for the convenience of explanation, the liquid level information at the first liquid level sensor 9, the second liquid level sensor 10, the third liquid level sensor 11 and the fourth liquid level sensor 12 are liquid levels one, two, three and four respectively, including:

[0054] If the first button is pressed, check the liquid level one, two, and three;

[0055] If no water is detected at level 1, 2, or 3, the first peristaltic pump 13 motor rotates to add NaOH dropwise, and the stirring motor rotates to stir;

[0056] If water is detected at level 1, but not at levels 2 and 3, the motor of the first peristaltic pump 13 stops rotating and the heating wire 6 starts heating;

[0057] After the heating stops, the second peristaltic pump 14 rotates to add the butanol-isoamyl alcohol mixed solution dropwise;

[0058] If water is detected at liquid level 1 and 2, but not at liquid level 3, the motor of the second peristaltic pump 14 stops rotating and the heating wire 6 starts heating;

[0059] After the heating stops, the stirring is stopped and the first solenoid valve 7 is opened;

[0060] If water is detected at level 3, but not at levels 1 and 2, the first solenoid valve 7 is closed and the motor of the third peristaltic pump 15 rotates to drip iodine reagent for 5 seconds;

[0061] If both the first button and the second button are pressed, the test continues and the second solenoid valve 8 is opened;

[0062] According to the preset time, the detection liquid will all flow into the waste liquid pool 3, the second solenoid valve 8 is closed, and the fourth peristaltic pump 16 motor rotates to add formic acid;

[0063] If the liquid level 4 detects water, the motor of the fourth peristaltic pump 16 stops rotating.

[0064] The motor of the third peristaltic pump 15 rotates to drop the iodine reagent and stops after 5 seconds.

[0065] Although the present disclosure is disclosed as above, the protection scope of the present disclosure is not limited thereto. Those skilled in the art may make various changes and modifications without departing from the spirit and scope of the present disclosure, and these changes and modifications will fall within the protection scope of the present invention.

Claims

1. A rapid starch detection automatic sampling device, characterized in that: include: Solution vessels, used to store test solutions at various stages of starch testing; A sensing device is provided in the solution container and is used to obtain liquid level information in the solution container; Control equipment, including reagent adding equipment, heating equipment, stirring equipment and valve control equipment; A control system for acquiring the liquid level information transmitted by the sensing device and driving the control device according to a preset program to complete the automated detection of starch; The solution vessel comprises a reaction pool (1), a detection pool (2), a waste liquid pool (3) and a sand core filter funnel (4); the valve control device comprises a first electromagnetic valve (7) and a second electromagnetic valve (8); the inlet of the reaction pool is used for adding starch to be detected; the outlet channel of the reaction pool passes through the first electromagnetic valve (7) and the sand core filter funnel in sequence to reach the inlet of the detection pool (2); and the outlet channel of the detection pool (2) passes through the second electromagnetic valve to reach the inlet of the waste liquid pool (3); The heating device comprises a heating wire (6), which is used to heat the solution in the reaction tank (1); the stirring device comprises a stirring rod (5), which is used to stir the solution in the reaction tank (1); The reagent dropping device comprises a first peristaltic pump (13), a second peristaltic pump (14), a third peristaltic pump (15) and a fourth peristaltic pump (16), wherein the first peristaltic pump (13) and the second peristaltic pump are connected to the reaction pool (1); The first peristaltic pump (13) is driven by a control system to drop NaOH solution into the reaction tank (1), and then the control system controls the movement of the heating wire (6) and the stirring rod (5) to disperse the starch paste in the reaction tank (1); The second peristaltic pump (14) is driven by a control system to drop a mixed solution of n-butanol and isoamyl alcohol into the reaction tank (1), and then heat, stir and cool the mixture to separate the amylose and amylopectin. The third peristaltic pump (15) is connected to the detection pool (2) and driven by the control system to drop iodine reagent into the detection pool (2) to complete the color development detection of the solution in the detection pool (2); The fourth peristaltic pump (16) is connected to the sand core filter funnel (4). When the reaction is completed and the solution in the reaction tank (1) flows through the sand core filter funnel (4), the amylose remains on the sand core filter screen of the sand core filter funnel (4). The fourth peristaltic pump (16) is used to add formic acid into the sand core filter funnel (4) to dissolve the amylose on the sand core filter screen. The sensing device comprises a first liquid level sensor (9), a second liquid level sensor (10), a third liquid level sensor (11) and a fourth liquid level sensor (12); The first liquid level sensor (9) and the second liquid level sensor (10) are arranged in the reaction tank (1) above and below. During the reaction, the control system obtains first liquid level information after the addition of NaOH solution through the first liquid level sensor (9), and obtains second liquid level information after the addition of n-butanol-isoamyl alcohol mixed solution through the second liquid level sensor (10), thereby controlling the closing of the first peristaltic pump (13) and the second peristaltic pump (14); The third liquid level sensor (11) and the fourth liquid level sensor (12) are arranged in the detection pool (2); the third liquid level sensor (11) obtains third liquid level information when the amylopectin solution enters the detection pool (2); the fourth liquid level sensor (12) obtains fourth liquid level information when the amylose enters the detection pool (2); the control system receives the third liquid level information and the fourth liquid level information and drives the third peristaltic pump (15) to drip a preset volume of iodine reagent into the detection pool (2).

2. The automatic sampling device for rapid starch detection according to claim 1, characterized in that The control system includes a power supply module, a main control chip, a first drive chip, a second drive chip, a liquid level sensing module, a button module, a heating module, a solenoid valve module, a stirring module and a reagent adding module.

3. The automatic sampling device for rapid starch detection according to claim 2, characterized in that The input end of the liquid level sensing module is respectively connected to a first liquid level sensor (9), a second liquid level sensor (10), a third liquid level sensor (11) and a fourth liquid level sensor (12), and the output end is respectively connected to four input ports of the main control chip. The key module includes a first key and a second key, and the first key and the second key are respectively connected to the input end of the main control chip.

4. The automatic sampling device for rapid starch detection according to claim 3, characterized in that The controlled ends of the first driving chip and the second driving chip are respectively connected to the output end of the main control chip, and the power supply end is connected to the power supply module. The output ends of the first driving chip are respectively connected to the heating module, the solenoid valve module and the stirring module. The heating module drives the heating wire (6), the solenoid valve module is used to drive the first solenoid valve (7) and the second solenoid valve (8), and the stirring module drives the stirring motor to drive the stirring rod (5); the four output ends of the second driving chip respectively drive the first peristaltic pump (13), the second peristaltic pump (14), the third peristaltic pump (15) and the fourth peristaltic pump (16).

5. A method for rapid detection of starch by automatic sampling, based on the automatic sampling device for rapid detection of starch according to any one of claims 1 to 4, characterized in that: include: Soluble starch is placed in the reaction tank (1), the power is turned on, and a start signal is given through the key module; The first peristaltic pump (13) motor rotates to add NaOH solution dropwise, and the stirring rod (5) starts stirring; When the first liquid level information indicates that there is water, the motor of the first peristaltic pump (13) stops rotating, and the heating wire (6) turns off after heating for a set time; The heating stops, the second peristaltic pump (14) motor rotates to drop the n-butanol-isoamyl alcohol mixed solution until the first liquid level information and the second liquid level information both show the presence of water, the second peristaltic pump (14) motor stops rotating, and the stirring rod (5) and the heating wire (6) are turned off after working for a set time; After the stirring rod (5) and the heating wire are turned off, the first electromagnetic valve (7) is opened, and the solution in the reaction tank (1) enters the detection tank (2) through the first electromagnetic valve (7) and the sand core filter funnel (4); Until the first liquid level information and the second liquid level information both indicate that there is no water and the third liquid level information indicates that there is water, the first electromagnetic valve (7) is closed and the motor of the third peristaltic pump (15) is rotated to drip the iodine reagent for 5 seconds and then stopped, thereby completing the pullulan solution detection; The main control system makes a judgment based on the information from the key module. If a detection signal of the amylose solution is received, the second solenoid valve (8) is opened for a preset time, so that all the solution in the detection tank (2) flows into the waste liquid tank (3), and the second solenoid valve (8) is closed; The fourth peristaltic pump (16) motor rotates to drip formic acid solution until the fourth liquid level information shows that there is water, the fourth peristaltic pump (16) motor stops rotating, and the third peristaltic pump (15) motor rotates to drip iodine reagent for 5 seconds and then stops, completing the amylose solution detection.

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