A diagnostic method and detection system based on the detection of autoimmunity disease antibodies

Abstract

The present invention relates to the technical field of antibody detection methods, and particularly relates to a diagnostic method and a detection system for detecting antibodies based on autoimmune diseases. The method includes the following steps: S1: The analysis module obtains relevant information on electrochemistry and relevant information on light reflection through electrochemical analysis and light reflection analysis, and transmits the relevant information on electrochemistry and the relevant information on light reflection to the control module; S2: The control module obtains an antibody concentration index based on the relevant information on electrochemistry and the relevant information on light reflection, and transmits the antibody concentration index to the diagnosis module; S3: The diagnosis module obtains information on strong or weak immune reactions based on the antibody concentration index, and transmits the information on strong or weak immune reactions to the communication module; S4: The communication module transmits the information on strong or weak immune reactions to the user terminal. By combining immunoassay with electrochemical analysis and light reflection analysis for detection, analyzing in different directions and combining the results can improve the accuracy and comprehensiveness of detection.

Description

Technical Field

[0001] The present invention relates to the technical field of antibody detection methods, and particularly to a diagnostic method and detection system for detecting antibodies of autoimmune diseases. Background Art

[0002] Antibody detection of autoimmune diseases is a method for diagnosing autoimmune diseases by detecting specific antibodies in human blood. In these diseases, the immune system mistakenly attacks its own healthy cells and tissues, producing antibodies that bind to autoantigens (i.e., self-cells or molecules). By detecting the presence and concentration of these antibodies, it can help identify and diagnose diseases such as systemic lupus erythematosus, rheumatoid arthritis, and thyroid autoimmune diseases. The detection of these antibodies usually uses immunological techniques such as enzyme-linked immunosorbent assay (ELISA), immunofluorescence, or chemiluminescence immunoassay to accurately identify and quantify the antibodies, thereby providing a basis for early diagnosis, monitoring, and treatment of diseases.

[0003] The application document with the publication number CN112114135A discloses an autoimmune detection system, belonging to the technical field of medical devices. It solves the problems in the prior art that medical staff need to continuously load materials on multiple devices, with long working hours and high working intensity. The present invention includes a fluorescence immunoassay analyzer, a first immunoblotting instrument, an enzyme immunoassay workstation, and a test tube substrate, and also includes a feeding device, a conveying mechanism, and a control device for controlling the feeding device and the conveying mechanism. The feeding device is respectively connected to the fluorescence immunoassay analyzer, the first immunoblotting instrument, and the enzyme immunoassay workstation. The feeding device has several manipulators for completing the feeding and discharging actions of the test tube substrate, and the manipulators can respectively send the test tube substrate into the fluorescence immunoassay analyzer, the first immunoblotting instrument, and the enzyme immunoassay workstation to complete the feeding operation.

[0004] The detection methods in the prior art are relatively single, resulting in large errors in the detected results. Summary of the Invention

[0005] The purpose of the present invention is to propose a diagnostic method and detection system for detecting antibodies of autoimmune diseases in view of the above-mentioned deficiencies.

[0006] The present invention adopts the following technical solutions:

[0007] A diagnostic method based on the detection of autoimmunity disease antibodies, the method comprising the following steps: S1: The analysis module obtains relevant electrochemical information and relevant optical reflection information through electrochemical analysis and optical reflection analysis, and transmits the relevant electrochemical information and relevant optical reflection information to the control module; S2: The control module obtains an antibody concentration index based on the relevant electrochemical information and relevant optical reflection information, and transmits the antibody concentration index to the diagnostic module; S3: The diagnostic module obtains information indicating a strong or weak immune response based on the antibody concentration index, and transmits the information indicating a strong or weak immune response to the communication module; S4: The communication module transmits the information indicating a strong or weak immune response to the user terminal.

[0008] The present invention also provides an autoimmunity disease antibody detection system applied to a diagnostic method based on the detection of autoimmunity disease antibodies. The system includes the analysis module, the control module, the diagnostic module and the communication module; the analysis module is used for analyzing and obtaining relevant electrochemical information and relevant optical reflection information, and transmitting the relevant electrochemical information and relevant optical reflection information to the control module; the control module obtains an antibody concentration index based on the relevant electrochemical information and relevant optical reflection information, and transmits the antibody concentration index to the diagnostic module; the diagnostic module obtains information indicating a strong or weak immune response based on the antibody concentration index, and transmits the information indicating a strong or weak immune response to the communication module; the communication module transmits the information indicating a strong or weak immune response to the user terminal.

[0009] Optionally, the analysis module includes an electrochemical analysis sub-module, an optical reflection analysis sub-module and an affinity analysis sub-module; the electrochemical analysis sub-module is used for analyzing and obtaining a bias current, a current before antigen-antibody binding and a current after antigen-antibody binding, and transmitting them to the control module; the optical reflection analysis sub-module is used for analyzing and obtaining an incident light intensity, a reflected light intensity, a wavelength of the incident light, a refractive index before antigen-antibody binding and a refractive index after antigen-antibody binding, and transmitting them to the control module; the affinity analysis sub-module is used for analyzing and obtaining a forward rate constant of antigen-antibody binding and a dissociation rate constant of antigen-antibody, and transmitting them to the control module; the control module obtains a forward rate factor of antigen-antibody binding based on the forward rate constant of antigen-antibody binding, obtains a refractive index change factor based on the refractive index before antigen-antibody binding and the refractive index after antigen-antibody binding, obtains a light attenuation degree factor based on the refractive index change factor and the wavelength of the incident light, obtains an antigen-antibody binding affinity factor based on the forward rate factor of antigen-antibody binding and the dissociation rate constant of antigen-antibody, obtains a current signal factor based on the current before antigen-antibody binding and the current after antigen-antibody binding, and obtains an antibody concentration index based on the current signal factor, the bias current, the antigen-antibody binding affinity factor, the incident light intensity, the reflected light intensity and the light attenuation degree factor.

[0010] Optionally, the light reflection analysis sub-module includes a light intensity analysis unit, a wavelength storage unit, and a refractive index analysis unit; the light intensity analysis unit is configured to analyze and obtain the incident light intensity and the reflected light intensity, and transmit them to the control module; the wavelength storage unit is configured to analyze and obtain the wavelength of the incident light, and transmit it to the control module; the refractive index analysis unit is configured to analyze and obtain the refractive index before antigen-antibody binding and the refractive index after antigen-antibody binding, and transmit them to the control module.

[0011] Optionally, when the control module calculates the antibody concentration index, the following formula is satisfied: ; where is the antibody concentration index, is the current signal factor, is the bias current, is the antigen-antibody binding affinity factor, is the incident light intensity, is the reflected light intensity, is the light attenuation degree factor.

[0012] The beneficial effects achieved by the present invention are as follows:

[0013] Combining immunoassay with electrochemical analysis and light reflection analysis for detection, analyzing from different directions and combining the results can improve the accuracy and comprehensiveness of detection;

[0014] The system adopts a modular design, and each sub-module (such as the electrochemical analysis sub-module, the light reflection analysis sub-module, etc.) can be independently upgraded or adjusted according to needs, and has good scalability and flexibility.

[0015] To further understand the features and technical content of the present invention, please refer to the following detailed description of the present invention and the attached drawings. However, the attached drawings are only provided for reference and illustration, and are not used to limit the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a flowchart of a diagnostic method for detecting antibodies based on autoimmune diseases according to the present invention;

[0017] Figure 2 is a schematic diagram of the overall structure of a detection system for detecting antibodies based on autoimmune diseases according to the present invention;

[0018] Figure 3 is a schematic diagram of the structure of the light reflection analysis sub-module in a detection system for detecting antibodies based on autoimmune diseases according to the present invention;

[0019] Figure 4 is an effect diagram of a detection system for detecting antibodies based on autoimmune diseases according to the present invention;

[0020] Figure 5 This is a schematic diagram of the overall structure of the second embodiment of an antibody detection system for autoimmune diseases according to the present invention;

[0021] Figure 6 This is a schematic diagram of the structure of the environmental analysis sub-module in the second embodiment of an antibody detection system for autoimmune diseases according to the present invention. Specific implementation manners

[0022] The following are specific embodiments to illustrate the implementation manners of the present invention. Those skilled in the art can understand the advantages and effects of the present invention from the content disclosed in this specification. The present invention can be implemented or applied through other different specific embodiments, and various details in this specification can also be modified and changed based on different viewpoints and applications without departing from the spirit of the present invention. Additionally, the drawings of the present invention are only for simple schematic illustration and are not drawn according to actual dimensions, with prior notice. The following implementation manners will further detail the relevant technical content of the present invention, but the disclosed content is not used to limit the protection scope of the present invention.

[0023] Combined with Figures 1 to 4 as shown.

[0024] Embodiment 1: This embodiment provides a diagnostic method for antibody detection of autoimmune diseases.

[0025] A diagnostic method for antibody detection of autoimmune diseases, the method includes the following steps. S1: The analysis module obtains relevant electrochemical information and relevant light reflection information through electrochemical analysis and light reflection analysis, and transmits the relevant electrochemical information and relevant light reflection information to the control module; S2: The control module obtains an antibody concentration index based on the relevant electrochemical information and relevant light reflection information, and transmits the antibody concentration index to the diagnostic module; S3: The diagnostic module obtains information on strong or weak immune reactions based on the antibody concentration index, and transmits the information on strong or weak immune reactions to the communication module; S4: The communication module transmits the information on strong or weak immune reactions to the user terminal.

[0026] Specifically, when the diagnostic module analyzes, the following principles are referred to: when the antibody concentration index is greater than or equal to the selected threshold of the antibody concentration index, it indicates a strong immune response; when the antibody concentration index is less than the selected threshold of the antibody concentration index, it indicates a weak immune response; the selected threshold of the antibody concentration index is set by those skilled in the art. It can be understood that autoimmune diseases refer to the situation where the immune system mistakenly attacks its own tissues. In this case, a strong immune response does not mean the presence of a pathogen outside, but rather an incorrect response of the internal immune system. For example, in diseases such as rheumatoid arthritis, lupus erythematosus, and multiple sclerosis, the abnormal activation of the immune system will lead to a strong immune response, attacking its own tissues and causing the occurrence of the disease. In this case, the manifestation of a strong immune response is exactly part of the disease.

[0027] This embodiment solves the problem that the traditional diagnostic methods are relatively single. By combining immunoassay with electrochemical analysis and optical reflection analysis for detection, analyzing from different directions and combining the results can improve the accuracy and comprehensiveness of the detection.

[0028] This embodiment also provides an antibody detection system for autoimmune diseases, which is applied to a diagnostic method for antibody detection of autoimmune diseases. The system includes the analysis module, the control module, the diagnostic module, and the communication module; the analysis module is used to analyze and obtain relevant electrochemical information and relevant optical reflection information, and transmit the relevant electrochemical information and relevant optical reflection information to the control module; the control module obtains the antibody concentration index according to the relevant electrochemical information and relevant optical reflection information, and transmits the antibody concentration index to the diagnostic module; the diagnostic module obtains information indicating a strong or weak immune response according to the antibody concentration index, and transmits the information indicating a strong or weak immune response to the communication module; the communication module transmits the information indicating a strong or weak immune response to the user terminal.

[0029] Optionally, the analysis module includes an electrochemical analysis sub-module, an optical reflection analysis sub-module, and an affinity analysis sub-module; the electrochemical analysis sub-module is configured to analyze and obtain the bias current, the current before antigen-antibody binding, and the current after antigen-antibody binding, and transmit them to the control module; the optical reflection analysis sub-module is configured to analyze and obtain the incident light intensity, the reflected light intensity, the wavelength of the incident light, the refractive index before antigen-antibody binding, and the refractive index after antigen-antibody binding, and transmit them to the control module; the affinity analysis sub-module is configured to analyze and obtain the forward rate constant of antigen-antibody binding and the rate constant of antigen-antibody dissociation, and transmit them to the control module; the control module obtains the forward rate factor of antigen-antibody binding according to the forward rate constant of antigen-antibody binding, obtains the refractive index change factor according to the refractive index before antigen-antibody binding and the refractive index after antigen-antibody binding, obtains the light attenuation degree factor according to the refractive index change factor and the wavelength of the incident light, obtains the antigen-antibody binding affinity factor according to the forward rate factor of antigen-antibody binding and the rate constant of antigen-antibody dissociation, obtains the current signal factor according to the current before antigen-antibody binding and the current after antigen-antibody binding, and obtains the antibody concentration index according to the current signal factor, the bias current, the antigen-antibody binding affinity factor, the incident light intensity, the reflected light intensity, and the light attenuation degree factor.

[0030] Optionally, the optical reflection analysis sub-module includes a light intensity analysis unit, a wavelength storage unit, and a refractive index analysis unit; the light intensity analysis unit is configured to analyze and obtain the incident light intensity and the reflected light intensity, and transmit them to the control module; the wavelength storage unit is configured to analyze and obtain the wavelength of the incident light, and transmit it to the control module; the refractive index analysis unit is configured to analyze and obtain the refractive index before antigen-antibody binding and the refractive index after antigen-antibody binding, and transmit them to the control module.

[0031] Optionally, when the control module calculates the antibody concentration index, the following formula is satisfied: ; where is the antibody concentration index, is the current signal factor, is the bias current, is the antigen-antibody binding affinity factor, is the incident light intensity, is the reflected light intensity, is the light attenuation degree factor.

[0032] Optionally, when the control module calculates, the following formula is satisfied: ; ; ; .

[0033] Where is the current before antigen-antibody binding, is the current after antigen-antibody binding; is the forward rate factor of antigen-antibody binding, is the rate constant of antigen-antibody dissociation; is the refractive index change factor, is the wavelength of the incident light; is the refractive index before antigen-antibody binding, is the refractive index after antigen-antibody binding.

[0034] When the control module calculates the antibody concentration index, refer to the following program code:

[0035] import math

[0036] # Define input parameters

[0037] # ΔI: Current signal factor

[0038] delta_I = float(input("Please enter ΔI (current signal factor): "))

[0039] # b: Bias current

[0040] b = float(input("Please enter b (bias current): "))

[0041] # K: Antigen-antibody binding affinity factor

[0042] KF = float(input("Please enter KF (forward rate factor of antigen-antibody binding): "))

[0043] kd = float(input("Please enter kd (rate constant of antigen-antibody dissociation): "))

[0044] K = KF / kd # Calculate K

[0045] # rs: Incident light intensity

[0046] rs = float(input("Please enter rs (incident light intensity): "))

[0047] # fs: Reflected light intensity

[0048] fs = float(input("Please enter fs (reflected light intensity): "))

[0049] # λ: Incident light wavelength

[0050] lambda_val = float(input("Please enter λ (wavelength of incident light, unit: nm):"))

[0051] # Refractive index change ΔN

[0052] n_before = float(input("Please enter n_before (refractive index before antigen-antibody binding):"))

[0053] n_after = float(input("Please enter n_after (refractive index after antigen-antibody binding):"))

[0054] delta_N = n_before - n_after

[0055] # Calculate α (light attenuation factor)

[0056] alpha = (4 * math.pi * delta_N) / lambda_val

[0057] # Calculate antibody concentration index C

[0058] C = 0.7 * ((delta_I - b) / (5 * K)) + 0.3 * (-math.log(1 + (fs / rs)) / (alpha * K))

[0059] # Output the result

[0060] print(f"The calculation result of antibody concentration index C is: {C}")

[0061] Specifically, when calculating the antibody concentration index, electrochemical analysis and optical reflection analysis experiments are required. Electrochemical analysis uses the relationship between current signals and antibody concentration to infer the antibody level, and the optical reflection analysis experiment uses the relationship between the intensity of reflected light and antibody concentration to infer the antibody level.

[0062] The unit of the bias current is microampere. The bias current refers to the initial current value detected in the absence of antibodies and antigens, which is usually caused by the inherent noise of the sensor, the measurement circuit, or environmental factors.

[0063] The incident light intensity refers to the initial light intensity detected in the absence of antibodies and antigens.

[0064] The reflected light intensity refers to the reflected light intensity after antigen-antibody binding.

[0065] The forward rate constant of antigen-antibody binding and the rate constant of antigen-antibody dissociation can be obtained through SPR experiments (in SPR experiments, light is incident on a metal surface (usually gold or silver), and surface plasmon waves are generated. When an antigen or antibody binds to a molecule on the metal surface, it causes a change in the surface refractive index. This change in refractive index affects the reflection angle of the incident light, and the change in reflection angle can be used to quantitatively analyze the binding and dissociation processes). The forward rate constant of antigen-antibody binding describes the binding rate between antigens and antibodies, and the rate constant of antigen-antibody dissociation describes the rate at which antigens and antibodies separate after the formation of the complex; the unit of the forward rate factor of antigen-antibody binding is milliliters / microgram·minute, and the unit of the rate constant of antigen-antibody dissociation is per minute; in this embodiment, the forward rate factor of antigen-antibody binding is equivalent to the directly detected forward rate constant of antigen-antibody binding.

[0066] The unit of the wavelength of the incident light is nanometers.

[0067] The refractive index before antigen-antibody binding and the refractive index after antigen-antibody binding can be obtained through SPR experiments (in SPR experiments, the incident light is irradiated onto the metal surface through an optical fiber or lens system, and surface plasmon waves are generated on the metal surface. The change in refractive index affects the reflection angle, so the change in refractive index can be inferred from the reflection angle).

[0068] The above units are just examples, and those skilled in the art can set different units according to actual needs when implementing this solution.

[0069] This embodiment solves the problem of low detection flexibility of traditional detection systems. The system adopts a modular design, and each sub-module (such as the electrochemical analysis sub-module, the optical reflection analysis sub-module, etc.) can be independently upgraded or adjusted according to needs, and has good scalability and flexibility.

[0070] Embodiment 2: This embodiment includes all the contents of Embodiment 1 and provides an antibody detection system for autoimmune diseases, as shown in combination with Figure 5 and Figure 6 as shown.

[0071] An antibody detection system for autoimmune diseases, and the analysis module further includes an environmental analysis sub-module;

[0072] The environmental analysis sub-module is used to analyze and obtain relevant information about the environment, and transmit the relevant information about the environment to the control module;

[0073] The control module obtains the forward rate factor of antigen-antibody binding based on the relevant information about the environment and the forward rate constant of antigen-antibody binding.

[0074] Optionally, the environmental analysis sub-module includes a temperature analysis unit, a humidity analysis unit, and a pH analysis unit; the temperature analysis unit is used to analyze and obtain the measured value and the ideal value of the environmental temperature, and transmit them to the control module; the humidity analysis unit is used to analyze and obtain the measured value and the ideal value of the environmental humidity, and transmit them to the control module; the pH analysis unit is used to analyze and obtain the measured value of the solution pH, the first ideal value of the solution pH, and the second ideal value of the solution pH, and transmit them to the control module; the control module obtains the pH index of the solution according to the measured value of the solution pH, the first ideal value of the solution pH, and the second ideal value of the solution pH, and obtains the forward rate factor of antigen-antibody binding according to the pH index of the solution, the forward rate constant of antigen-antibody binding, the measured value of the environmental temperature, the ideal value of the environmental temperature, the measured value of the environmental humidity, and the ideal value of the environmental humidity.

[0075] Optionally, when the control module calculates the forward rate factor of antigen-antibody binding, the following formula is satisfied:

[0076] ; 。

[0077] Wherein, is the forward rate constant of antigen-antibody binding, is the measured value of the environmental temperature, is the ideal value of the environmental temperature, is the measured value of the environmental humidity, is the ideal value of the environmental humidity, is the pH index of the solution; is the measured value of the solution pH, is the first ideal value of the solution pH, is the second ideal value of the solution pH.

[0078] When the control module calculates the forward rate factor of antigen-antibody binding, the following program code is referenced:

[0079] # Define input parameters

[0080] # Environmental temperature and humidity related parameters

[0081] temp_ec = float(input("Please enter temp_ec (measured value of environmental temperature, unit: °C): "))

[0082] temp_ref = float(input("Please enter temp_ref (ideal value of environmental temperature, unit: °C): "))

[0083] sd_ec = float(input("Please enter sd_ec (measured value of environmental humidity, unit: %):"))

[0084] sd_ref = float(input("Please enter sd_ref (ideal value of environmental humidity, unit: %):"))

[0085] # Parameters related to the pH of the solution

[0086] ph_ec = float(input("Please enter ph_ec (measured value of the pH of the solution):"))

[0087] ph1 = float(input("Please enter ph1 (first ideal value of the pH of the solution):"))

[0088] ph2 = float(input("Please enter ph2 (second ideal value of the pH of the solution):"))

[0089] # Related to the forward rate constant

[0090] k_f = float(input("Please enter k_f (forward rate constant of antigen-antibody binding, unit: mL / μg·min):"))

[0091] # Define the calculation of the solution pH factor SJ

[0092] if ph1 <= ph_ec <= ph2:

[0093] SJ = 1 # Within the ideal range, the pH factor is 1

[0094] else:

[0095] SJ = 1.1 # Not within the ideal range, the pH factor is 1.1

[0096] # Calculate the forward rate factor KF of antigen-antibody binding

[0097] KF = k_f * ((1 - (temp_ec - temp_ref) / temp_ref) * (1 + abs(sd_ec -sd_ref) / (sd_ec + sd_ref)) * SJ)

[0098] # Output the calculation result

[0099] print(f"The calculation result of the forward rate factor KF of antigen-antibody binding is: {KF}")

[0100] Specifically, when the accuracy of the experiment is required to be high, the forward rate constant of the antigen-antibody binding needs to be corrected and compensated.

[0101] The forward rate constant for antigen-antibody binding is expressed in milliliters per microgram per minute.

[0102] The units of the measured value of ambient temperature and the ideal value of ambient temperature are both degrees Celsius. The ideal value of ambient temperature is set by technical personnel in this field. When the measured value of ambient temperature is greater than the ideal value of ambient temperature, the temperature increase will increase the kinetic energy of molecules, increase the frequency of collisions between molecules, and accelerate the binding reaction rate, which will lead to a larger forward rate constant for antigen-antibody binding obtained from the test; when the measured value of ambient temperature is less than the ideal value of ambient temperature, low temperature will lead to a decrease in the kinetic energy of molecules, a decrease in the frequency of collisions between antigens and antibodies, and a slower reaction rate, which will lead to a smaller forward rate constant for antigen-antibody binding obtained from the test. The forward rate constant is adjusted under the current ambient temperature conditions so that the antibody concentration index meets the preset range, thereby obtaining a test that meets the current ambient temperature conditions. .

[0103] When the measured value of ambient humidity is greater than or less than the ideal value of ambient humidity, excessive humidity may cause the sensor electrode or antibody surface to absorb water, change the antibody's conformation or its adsorption capacity on the surface, and cause the reaction rate to decrease. Under low humidity, insufficient moisture on the sensor surface or in the reaction medium may cause adverse changes in the antibody-antigen binding site, or reduce the antibody's adsorption capacity and slow the reaction. Both of the above situations will cause the forward rate constant of antigen-antibody binding obtained from the test to be too small. Adjust the forward rate constant under the current ambient humidity conditions so that the antibody concentration index meets the preset range, thereby obtaining a value that meets the current ambient humidity conditions. .

[0104] The first ideal value of the solution pH and the second ideal value of the solution pH are set by those skilled in the art, and the solution refers to the solution after the antigen and antibody are mixed; when the measured value of the solution pH is less than the first ideal value of the solution pH, the amino groups or other charged groups on the surface of the antigen and antibody may be protonated, changing the conformation of the antibody-antigen binding site, resulting in a smaller forward rate constant for antigen-antibody binding obtained by the test; when the measured value of the solution pH is greater than the second ideal value of the solution pH, the amino or carboxyl ion groups of the antigen and antibody will lose their charge, affecting the electrostatic interaction between the antigen and antibody, and the affinity of the antibody may be reduced, thereby affecting the binding rate between the antigen and the antibody, resulting in a smaller forward rate constant for antigen-antibody binding obtained by the test. The forward rate constant is adjusted under the solution pH condition so that the antibody concentration index meets the preset range, thereby obtaining a pH value that meets the current solution pH. 。

[0105] After obtaining three forward rate constants that meet the three conditions according to the three conditions of ambient temperature, ambient humidity, and solution pH respectively, divide the three obtained forward rate constants by three to obtain the final forward rate constant.

[0106] The above units are only examples, and those skilled in the art can set different units according to actual needs when implementing this solution.

[0107] This embodiment solves the problem of relatively low detection accuracy of traditional detection systems, and improves the overall detection accuracy by correcting the forward rate constant of antigen-antibody binding.

[0108] The content disclosed above is only the preferred feasible embodiment of the present invention, and does not limit the protection scope of the present invention. Therefore, all equivalent technical changes made by using the content of the specification and drawings of the present invention are included in the protection scope of the present invention. In addition, the elements therein can be updated with the development of technology.

Claims

1. An antibody detection system for autoimmune diseases, the system comprising an analysis module, a control module, a diagnosis module and a communication module; The analysis module includes an electrochemical analysis sub-module, an optical reflection analysis sub-module and an affinity analysis sub-module; The electrochemical analysis sub-module is used to analyze and obtain the bias current, the current before antigen-antibody binding and the current after antigen-antibody binding, and transmit them to the control module; The optical reflection analysis sub-module is used to analyze and obtain the incident light intensity, the reflected light intensity, the wavelength of the incident light, the refractive index before antigen-antibody binding and the refractive index after antigen-antibody binding, and transmit them to the control module; The affinity analysis sub-module is used to analyze and obtain the forward rate constant of antigen-antibody binding and the rate constant of antigen-antibody dissociation, and transmit them to the control module; wherein, The forward rate constant of antigen-antibody binding describes the binding rate between antigens and antibodies, and the rate constant of antigen-antibody dissociation describes the rate at which antigens and antibodies separate after the formation of the complex; The control module obtains the forward rate factor of antigen-antibody binding according to the forward rate constant of antigen-antibody binding, obtains the refractive index change factor according to the refractive index before antigen-antibody binding and the refractive index after antigen-antibody binding, obtains the light attenuation degree factor according to the refractive index change factor and the wavelength of the incident light, obtains the antigen-antibody binding affinity factor according to the forward rate factor of antigen-antibody binding and the rate constant of antigen-antibody dissociation, obtains the current signal factor according to the current before antigen-antibody binding and the current after antigen-antibody binding, and obtains the antibody concentration index according to the current signal factor, the bias current, the antigen-antibody binding affinity factor, the incident light intensity, the reflected light intensity and the light attenuation degree factor; The analysis module further includes an environmental analysis sub-module; the environmental analysis sub-module includes a temperature analysis unit, a humidity analysis unit and a pH analysis unit; the temperature analysis unit is used to analyze and obtain the measured value and the ideal value of the environmental temperature, and transmit them to the control module; the humidity analysis unit is used to analyze and obtain the measured value and the ideal value of the environmental humidity, and transmit them to the control module; the pH analysis unit is used to analyze and obtain the measured value of the solution pH, the first ideal value of the solution pH and the second ideal value of the solution pH, and transmit them to the control module; When the control module calculates the antibody concentration index, it satisfies the following formula: ； Among them, is the antibody concentration index, is the current signal factor, is the bias current, is the antigen-antibody binding affinity factor, is the incident light intensity, is the reflected light intensity, is the light attenuation degree factor; When the control module calculates the forward rate factor of antigen-antibody binding, it satisfies the following formula: ； ； Among them, is the forward rate constant of antigen-antibody binding, is the measured value of the environmental temperature, is the ideal value of the environmental temperature, is the measured value of the environmental humidity, is the ideal value of the environmental humidity, is the pH index of the solution; is the measured value of the solution pH, is the first ideal value of the solution pH, is the second ideal value of the solution pH.

2. The autoimmune disease antibody detection system according to claim 1, wherein The optical reflection analysis sub-module includes a light intensity analysis unit, a wavelength storage unit and a refractive index analysis unit; The light intensity analysis unit is used to analyze and obtain the incident light intensity and the reflected light intensity, and transmit them to the control module; The wavelength storage unit is used to analyze and obtain the wavelength of the incident light, and transmit them to the control module; The refractive index analysis unit is used to analyze and obtain the refractive index before antigen-antibody binding and the refractive index after antigen-antibody binding, and transmit them to the control module.

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