Equipment capable of detecting various proteins in plasma

Abstract

The invention discloses equipment capable of detecting various proteins in plasma, and relates to the technical field of blood detection.The equipment comprises a detection rod, and a groove body is formed in the top of the detection rod; the groove body comprises a sample adding area and a neodymium magnet area; a discharge port is formed in the side end of the tank body and is used for discharging liquid; an insertion opening is formed in the side face of the detection box and used for insertion of the detection rod; a liquid crystal screen is assembled at the top of the detection box, a detection switch is further assembled at the top of the detection box, and a photomultiplier is arranged above the insertion opening; a main control circuit board is further arranged in the detection box and used for controlling all electric components in the detection box, the whole detection process only needs to be carried out in the small detection box and several additional EP tubes, large precise instruments do not need to be used, and the detection box has low cost and high generalization performance. The blood concentration levels of a plurality of protein markers are roughly measured at the same time, and the severity of corresponding diseases can be represented by the total luminous intensity, so that disease grading is established.

Description

Technical Field

[0001] The present invention relates to the technical field of blood testing, and more particularly to a device capable of detecting multiple proteins in plasma. Background Art

[0002] Blood testing is a medical procedure that analyzes various components of a blood sample to diagnose disease, assess health status, and monitor the effectiveness of treatment.

[0003] There are many ways to detect various proteins in plasma. Here are some common methods:

[0004] 1. Protein electrophoresis: This is a commonly used method for protein separation and analysis. Proteins are separated in gel according to their size, charge and other characteristics through electrophoresis, and then the proteins are quantitatively or qualitatively analyzed by staining or other detection methods.

[0005] 2. Immunoassay: Immunoassay uses the specific binding of antibodies to target proteins to detect the presence and concentration of proteins. Common immunoassay methods include ELISA (enzyme-linked immunosorbent assay), Western blotting, and immunofluorescence.

[0006] 3. Mass spectrometry: Mass spectrometry is a high-precision protein analysis method that can detect and quantify a variety of proteins in plasma. It ionizes protein molecules and performs mass analysis to determine the molecular weight and composition of proteins.

[0007] 4. Protein chip: Protein chip is a high-throughput protein detection technology that fixes a large number of proteins on the chip surface and then reacts with plasma samples to determine the presence and concentration of proteins in plasma by detecting signals on the chip.

[0008] These methods have their own advantages and disadvantages, and the appropriate method can be selected according to specific needs and research purposes. When conducting protein testing, attention should be paid to sample collection, processing and preservation to ensure the accuracy and reliability of the test results. In addition, the detection of proteins in plasma has important application value in medical diagnosis, disease monitoring, drug development and other fields. By detecting changes in specific proteins in plasma, it can help doctors diagnose diseases, evaluate treatment effects, monitor disease progression, etc.

[0009] However, most of these detection methods require the use of large equipment or can only be completed in specific places (hospitals, physical examination centers, etc.), and cannot be completed at home. Based on the above problems, we provide a device that can detect multiple proteins in plasma.

[0010] The above information disclosed in this background technology is only used to increase the understanding of the background technology of the present invention and therefore, it may include information that does not constitute the prior art known to a person of ordinary skill in the art. Summary of the invention

[0011] In order to solve the problems raised in the above background technology, the present invention provides a device capable of detecting multiple proteins in plasma.

[0012] The present invention provides a device capable of detecting multiple proteins in plasma using the following technical solution:

[0013] A device capable of detecting multiple proteins in plasma comprises a detection rod, the top of which is provided with a groove; the groove comprises a sample addition area and a neodymium magnet area; a discharge port is provided at the side end of the groove for discharging liquid; a detection box, the side of which is provided with an insertion port for inserting the detection rod; a liquid crystal screen is mounted on the top of the detection box, a detection switch is also mounted on the top of the detection box, and a photomultiplier tube is arranged above the insertion port; a main control circuit board is also arranged inside the detection box for controlling various electrical components in the detection box.

[0014] Preferably, the interior of the detection box is also equipped with a battery box for supplying power to various electrical components in the detection box, and the side of the detection box is equipped with a switch.

[0015] Preferably, the trough body is an inclined structure for the flow of fluid.

[0016] Preferably, the photomultiplier tube is electrically connected to the battery box, the switch and the liquid crystal screen through a main control circuit board by wires.

[0017] Preferably, two track grooves are provided inside the groove body, and a push plate is slidably assembled between the two track grooves.

[0018] Preferably, a recess is provided at the bottom of the push plate, and the inner wall of the recess is rotatably connected to two rollers, a polygonal strip is inserted and assembled between the two rollers, a hair roller is passed through the middle of the polygonal strip, and the hair roller is eccentrically arranged on the polygonal strip.

[0019] Preferably, a placement groove is provided on the side of the push plate, and a pull ring is slidably connected inside the placement groove.

[0020] A method for using a device capable of detecting multiple proteins in plasma comprises the following steps:

[0021] S1. Drop the blood collected from the fingertips into the first EP tube. After natural sedimentation, use a dropper to absorb the supernatant and add it to the EP tube for antibody incubation. Mix well and oscillate for one minute. The tube has pre-stored enzyme-linked antigens of various antibodies to be detected. The enzyme-linked antigen is a fluorescent protein antibody. Use a dropper to transfer the liquid in the EP tube for antibody incubation to the EP tube for forming an antigen-antibody-magnetic bead complex. Mix well and oscillate for one minute. The tube has pre-stored magnetic bead-adsorbed antibodies of various antigens to be detected.

[0022] S2, then add the mixed reagents to the sample adding area in the tank body. When the reagents slowly flow through, the target protein will be adsorbed by the neodymium magnet in the neodymium magnet area. Then add distilled water to the sample adding area to wash away the remaining protein impurities. The excess liquid will flow out through the discharge port.

[0023] S3. Turn on the switch of the test box, mix the luminol reagent with the protein-fixed slot, then insert the test stick into the insertion port of the test box and press the test switch. The luminescence intensity of the sample can be detected by the photomultiplier tube, and converted into an electrical signal by the main control circuit board, and finally displayed on the LCD screen. The data and related scales can be used to characterize the overall protein level to determine the level of certain diseases.

[0024] In summary, the present invention includes the following beneficial technical effects:

[0025] 1. By turning on the switch of the test box, mixing the luminol reagent in the groove where the protein has been fixed, then inserting the test stick into the insertion port of the test box and pressing the test switch, the luminescence intensity of the sample can be detected by the photomultiplier tube, and converted into an electrical signal by the main control circuit board, and finally displayed on the LCD screen. The entire detection process only needs to be carried out in a small test box and several additional EP tubes, without the need for large precision instruments, with low cost and high promotion; at the same time, the blood concentration levels of multiple protein markers can be roughly measured, and the severity of the corresponding disease can be characterized by the total luminescence intensity, so as to establish disease classification.

[0026] 2. Pull the pull ring so that the pull ring is arranged on the top of the push plate, and then push the pull ring to move the push plate in the slot body. When the push plate moves, the two rollers will move in the slot body, thereby driving the polygonal strips to rotate, and then the wool roller can rotate in the slot body and clean the impurities and residues in the slot body. The wool roller is eccentrically arranged, and when facing the concave and convex surfaces in the slot body, it can also clean them. At the same time, when the two rollers move to the end section of the slot body, the wool roller can be eccentrically swung, so that the impurities on the wool roller can fall off through centrifugal force.

[0027] 3. When the two rollers rotate, the corresponding polygonal strips will be driven to rotate, so that the push plate rotates in the push plate, thereby cooperating with the spring telescopic rod to make the movable panel move up and down in the push plate, and squeeze the airbag, so that the jet pipe blows air into the tank body. Through this structural design, when the wool roller cleans the tank body, the tank body can also be sprayed with air, so that the impurities in the tank body and the inner wall of the tank body fall off, which is more conducive to the wool roller cleaning and impurities adhering to the tank body.

[0028] The above summary is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments and features described above, further aspects, embodiments and features of the present invention will be readily apparent by reference to the accompanying drawings and the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 is a schematic diagram of the structure of a device capable of detecting multiple proteins in plasma in an embodiment of the present invention;

[0030] Figure 2 is a schematic diagram of the other side structure of a device capable of detecting multiple proteins in plasma in an embodiment of the present invention;

[0031] Figure 3 is a schematic structural diagram of a detection rod in an embodiment of the present invention;

[0032] Figure 4 is a schematic structural diagram of the other side of the detection rod in an embodiment of the present invention;

[0033] Figure 5 is a schematic structural diagram of a push plate in an embodiment of the present invention;

[0034] Figure 6 is a schematic structural diagram of the other side of the push plate in an embodiment of the present invention;

[0035] Figure 7 is a schematic diagram of the structure of an air bag, an air intake pipe and an exhaust pipe in an embodiment of the present invention;

[0036] Figure 8 It is a schematic structural diagram of the other side of the airbag, the air intake pipe and the exhaust pipe in an embodiment of the present invention.

[0037] Description of reference numerals: 1. Detection rod; 100. trough body; 101. sample adding area; 102. neodymium magnet area; 103. discharge port; 104. track slot; 105. push plate; 106. notch; 107. roller; 108. polygonal strip; 109. hair roller; 110. placement slot; 111. pull ring; 2. detection box; 3. insertion port; 4. LCD screen; 5. detection switch; 6. switch; 7. photomultiplier tube; 8. battery box; 9. push plate; 10. movable panel; 11. spring telescopic rod; 12. air bag; 13. air intake pipe; 14. jet pipe. DETAILED DESCRIPTION

[0038] The following is combined with Figures 1 to 8 The present invention is described in further detail.

[0039] It should be noted that the drawings are schematic and not to scale. For the sake of clarity and convenience, the relative sizes and proportions of the parts shown in the drawings are exaggerated or reduced in size, and any size is only illustrative and not restrictive. In addition, the same reference symbol is used for the same structure, element or accessory appearing in more than two figures to reflect similar features.

[0040] The present invention discloses a device capable of detecting multiple proteins in plasma. Figures 1 to 6 A device capable of detecting multiple proteins in plasma comprises a detection rod 1, a groove body 100 is provided on the top of the detection rod 1; the groove body 100 comprises a sample adding area 101 and a neodymium magnet area 102; a discharge port 103 is provided on the side end of the groove body 100 for discharging liquid; a detection box 2, an insertion port 3 is provided on the side of the detection box 2 for inserting the detection rod 1; a liquid crystal screen 4 is provided on the top of the detection box 2, a detection switch 5 is also provided on the top of the detection box 2, and a photomultiplier tube 7 is provided above the insertion port 3; a main control circuit board is also provided inside the detection box 2 for controlling various electrical components in the detection box 2.

[0041] Specifically, a battery box 8 is installed inside the detection box 2 for supplying power to various electrical components in the detection box 2 , and a switch 6 is installed on the side of the detection box 2 .

[0042] Specifically, the tank body 100 is an inclined structure for the flow of fluid.

[0043] Specifically, the photomultiplier tube 7 is electrically connected to the battery box 8, the switch 6 and the liquid crystal screen 4 through a main control circuit board by wires.

[0044] Specifically, two track grooves 104 are provided inside the groove body 100 , and a push plate 105 is slidably assembled between the two track grooves 104 .

[0045] Specifically, a recess 106 is provided at the bottom of the push plate 105, and two rollers 107 are rotatably connected to the inner wall of the recess 106. A polygonal strip 108 is inserted and assembled between the two rollers 107. A hair roller 109 is passed through the middle of the polygonal strip 108, and the hair roller 109 is eccentrically arranged on the polygonal strip 108.

[0046] Specifically, a placement groove 110 is formed on the side of the push plate 105 , and a pull ring 111 is slidably connected inside the placement groove 110 .

[0047] When cleaning the trough body 100, the pull ring 111 is pulled so that the pull ring 111 is arranged on the top of the push plate 105, and then the pull ring 111 is pushed to move the push plate 105 in the trough body 100. When the push plate 105 moves, the two rollers 105 will move in the trough body 100, thereby driving the polygonal strip 108 to rotate, and then the hair roller 109 can rotate in the trough body 100 and clean the impurities and residues in the trough body 100. Moreover, the hair roller 109 is eccentrically arranged, and when facing the concave and convex surfaces in the trough body 100, it can also be cleaned. At the same time, when the two rollers 105 move to the end section of the trough body 100 (the end section of the track groove 104 is a Z-shaped structure, so that the two rollers 105 are separated from the trough body 100), the hair roller 109 can be eccentrically swung, so that the impurities on the hair roller 109 can fall off due to centrifugal force.

[0048] like Figure 7 and Figure 8 As shown, a push plate 9 is eccentrically provided at the middle part of the two polygonal strips 108, and the two push plates 9 are arranged inside the push plate 105. A movable panel 10 is provided inside the push plate 105 relative to the position of the two push plates 9. The movable panel 10 is assembled inside the push plate 105 through a spring telescopic rod 11, and an airbag 12 is provided inside the push plate 105 at a position above the movable panel 10. One side of the airbag 12 is connected to a plurality of air intake pipes 13, and the other side of the airbag 12 is connected to a plurality of air injection pipes 14.

[0049] Specifically, the air intake pipe 13 and the air injection pipe 14 are both one-way pipes. The port of the air injection pipe 14 is vertically aligned with the slot body 100 for discharging the gas in the airbag 12 . The port of the air intake pipe 13 is arranged outside the push plate 15 for sucking air into the airbag 12 .

[0050] Specifically, during the rotation of the two push plates 9 , the movable panel 10 can be pushed up and down, and the spring telescopic rods 11 can be squeezed.

[0051] When the two rollers 107 rotate, the corresponding polygonal strips 108 will be driven to rotate, so that the push plate 9 rotates in the push plate 105, thereby cooperating with the spring telescopic rod 11 to make the movable panel 10 move up and down in the push plate 105 and squeeze the airbag 12, so that the air jet 14 blows air into the trough body 100. Through this structural design, when the wool roller 109 cleans the trough body 100, the trough body 100 can also be sprayed with air, so that the impurities in the trough body 100 fall off from the inner wall of the trough body 100, which is more conducive to the wool roller 109 to clean and adhere to the impurities on the trough body 100.

[0052] A method for using a device capable of detecting multiple proteins in plasma comprises the following steps:

[0053] S1. Drop the blood collected from the fingertips into the first EP tube. After natural sedimentation, use a dropper to absorb the supernatant and add it to the EP tube for antibody incubation. Mix well and oscillate for one minute. The tube has pre-stored enzyme-linked antigens of various antibodies to be detected. The enzyme-linked antigen is a fluorescent protein antibody. Use a dropper to transfer the liquid in the EP tube for antibody incubation to the EP tube for forming an antigen-antibody-magnetic bead complex. Mix well and oscillate for one minute. The tube has pre-stored magnetic bead-adsorbed antibodies of various antigens to be detected.

[0054] S2, then add the mixed reagents to the sample adding area 101 in the tank body 100, when the reagents slowly flow through, the target protein will be adsorbed by the neodymium magnet in the neodymium magnet area 102, then add distilled water to the sample adding area 101 to wash away the remaining protein impurities, and the excess liquid will flow out through the discharge port 103;

[0055] S3, turn on the switch 6 of the detection box 2, mix the luminol reagent with the groove 100 where the protein has been fixed, then insert the detection stick 1 into the insertion port 3 of the detection box 2, and press the detection switch 5, the luminescence intensity of the sample can be detected by the photomultiplier tube 7, and converted into an electrical signal by the main control circuit board, and finally displayed on the LCD screen 4. The level of overall protein can be represented by the data and related scales to determine the level of certain diseases.

[0056] The standard parts used in the present invention can all be purchased from the market, and special-shaped parts can be customized according to the description in the specification and the drawings. The specific connection methods of each part adopt conventional means such as mature bolts, rivets, welding, etc. in the prior art. The machinery, parts and equipment all adopt conventional models in the prior art, and the circuit connection adopts the conventional connection method in the prior art, which will not be described in detail here.

[0057] In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. "Multiple" means two or more, unless otherwise clearly and specifically defined.

[0058] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0059] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0060] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, unless they are contradictory.

[0061] In the drawings of the embodiments disclosed in the present invention, only the structures related to the embodiments disclosed in the present invention are involved, and other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present invention can be combined with each other.

[0062] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A device capable of detecting multiple proteins in plasma, characterized in that: include: A detection rod (1), wherein a groove (100) is provided on the top of the detection rod (1); The tank body (100) comprises a sample adding area (101) and a neodymium magnet area (102); The side end of the tank body (100) is provided with a discharge port (103) for discharging liquid; A detection box (2), wherein a side of the detection box (2) is provided with an insertion opening (3) for inserting the detection stick (1); The top of the detection box (2) is equipped with a liquid crystal screen (4), the top of the detection box (2) is also equipped with a detection switch (5), and a photomultiplier tube (7) is arranged above the insertion port (3); A main control circuit board is also provided inside the detection box (2) for controlling various electrical components inside the detection box (2).

2. A device for detecting multiple proteins in plasma according to claim 1, characterized in that: The detection box (2) is also equipped with a battery box (8) inside for supplying power to various electrical components in the detection box (2), and a switch (6) is installed on the side of the detection box (2).

3. A device for detecting multiple proteins in plasma according to claim 1, characterized in that: The trough body (100) is of an inclined structure and is used for the flow of fluid.

4. A device for detecting multiple proteins in plasma according to claim 1, characterized in that: The photomultiplier tube (7) is electrically connected to the battery box (8), the switch (6) and the liquid crystal screen (4) via a main control circuit board through wires.

5. The device for detecting multiple proteins in plasma according to claim 1, characterized in that: Two track grooves (104) are provided inside the groove body (100), and a push plate (105) is slidably assembled between the two track grooves (104).

6. A device for detecting multiple proteins in plasma according to claim 5, characterized in that: A notch (106) is provided at the bottom of the push plate (105), and two rollers (107) are rotatably connected to the inner wall of the notch (106). A polygonal strip (108) is inserted and assembled between the two rollers (107), and a hair roller (109) is passed through the middle of the polygonal strip (108), and the hair roller (109) is eccentrically arranged on the polygonal strip (108).

7. A device for detecting multiple proteins in plasma according to claim 6, characterized in that: A placement groove (110) is provided on the side of the push plate (105), and a pull ring (111) is slidably connected inside the placement groove (110).

8. A method for using a device capable of detecting multiple proteins in plasma, characterized in that: The following steps are involved: S1. Drop the blood collected from the fingertips into the first EP tube. After natural sedimentation, use a dropper to absorb the supernatant and add it to the EP tube for antibody incubation. Mix well and oscillate for one minute. The tube has pre-stored enzyme-linked antigens of various antibodies to be detected. The enzyme-linked antigen is a fluorescent protein antibody. Use a dropper to transfer the liquid in the EP tube for antibody incubation to the EP tube for forming an antigen-antibody-magnetic bead complex. Mix well and oscillate for one minute. The tube has pre-stored magnetic bead-adsorbed antibodies of various antigens to be detected. S2, then adding the mixed reagents to the sample adding area (101) in the tank body (100), when the reagents slowly flow through, the target protein will be adsorbed by the neodymium magnet in the neodymium magnet area (102), then adding distilled water to the sample adding area (101) to wash away the remaining protein impurities, and the excess liquid will flow out through the discharge port (103); S3. Turn on the switch (6) of the detection box (2), mix the luminol reagent with the groove body (100) where the protein has been fixed, then insert the detection stick (1) into the insertion port (3) of the detection box (2), and press the detection switch (5). The luminescence intensity of the sample can be detected by the photomultiplier tube (7), and converted into an electrical signal by the main control circuit board, and finally displayed on the liquid crystal screen (4). The data and related scales can be used to characterize the level of overall protein to determine the level of certain diseases.

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