Microfluidic chip and microfluidic analysis system

Abstract

The invention is suitable for the field of medical equipment, and discloses a micro-fluidic chip and a micro-fluidic analysis system. The micro-fluidic chip comprises a non-circular chip main body, wherein a sample injection cavity, a first sample quantification cavity, a diluent inlet cavity, a diluent quantification cavity, a mixing cavity, a distribution cavity, a reaction detection cavity, a first overflow cavity and a second overflow cavity are formed in the non-circular chip main body; the reaction detection cavity is used for reacting a reagent with the mixed liquid to form a sample; the first overflow cavity is respectively communicated with the diluent quantifying cavity and the distribution cavity so as to be used for collecting the diluent overflowing from the diluent quantifying cavity and collecting the mixed liquid overflowing from the distribution cavity; the second overflow cavity is communicated with the first sample quantification cavity and is used for collecting samples overflowing from the first sample quantification cavity. The micro-fluidic chip is simple and convenient to use and operate, the cost of sample reexamination can be reduced, and waste of unnecessary samples and consumables is reduced.

Description

Microfluidic chips and microfluidic analysis systems Technical Field

[0001] The present application relates to the field of medical equipment, and in particular to a microfluidic chip and a microfluidic analysis system having the microfluidic chip. Background Art

[0002] Microfluidic analysis technology integrates the basic operating units of the sample analysis process, such as sample addition, separation, dilution, reaction, and detection, into a microfluidic chip with microchannels (with a size of tens to hundreds of microns), automatically completing the entire process of sample analysis.

[0003] The microfluidic chip provided by traditional technology is a complete circular sheet. The microfluidic chip is surrounded by multiple reaction detection cavities along the circumference, and each reaction detection cavity is used to carry the sample to perform a test item. When the complete circular microfluidic chip is used for sample testing, it only has the package detection function (that is, the test items of each reaction detection cavity of the microfluidic chip are tested as a package). When medical staff question the test results of a certain test item in the package, or when the test results of a certain test item are biased, the medical staff needs to review. When testing a single, two or three test items, using a complete circular microfluidic chip for review will cause most of the reaction detection cavities on the microfluidic chip to be redundant. This will increase the cost of the review on the one hand, and on the other hand, it will lead to the waste of redundant reaction detection cavities on the microfluidic chip. On the other hand, more samples need to be consumed, resulting in unnecessary waste of samples, diluents and reagents.

[0004] In order to solve the above technical problems, the related art provides a fan-shaped microfluidic chip, wherein a plurality of fan-shaped microfluidic chips can be spliced ​​into a complete circular microfluidic structure, and the fan-shaped microfluidic chip can reduce the waste of unnecessary reaction detection chambers, samples, diluents and reagents to a certain extent. However, due to the unreasonable structural layout of the fan-shaped microfluidic chip, in specific applications, there are still the following deficiencies: (1) The fan-shaped microfluidic chip does not have the function of separating whole blood samples, and does not have the function of quantitative sample and diluent, which makes the operation of using the fan-shaped microfluidic chip to detect whole blood samples more complicated. It is necessary to first centrifuge the whole blood, take out a certain amount of plasma and add it to the diluent tube, invert and mix it several times, and then take out a certain amount of the mixed liquid and add it to the fan-shaped microfluidic chip. (2) The central angle of the fan-shaped microfluidic chip is 120°, and it is difficult to further miniaturize it with this layout. There are still many reaction detection chambers on it, which will still cause waste of reaction detection chambers, samples, diluents and reagents.

[0005] Summary of the Invention

[0006] The first purpose of the present application is to provide a microfluidic chip, which aims to solve the technical problems in the related art of high cost and serious waste of samples and consumables when using microfluidic chips for review.

[0007] To achieve the above-mentioned object, the present application provides a solution: a microfluidic chip, characterized in that: it includes a non-circular chip body, wherein the non-circular chip body is formed with a sample injection chamber, a first sample quantitative chamber, a diluent inlet chamber, a diluent quantitative chamber, a mixing chamber, a distribution chamber, a reaction detection chamber, a first overflow chamber, and a second overflow chamber;

[0008] The injection chamber is used to store samples entering the microfluidic chip;

[0009] The first sample quantification chamber is in communication with the injection chamber, so as to quantify the sample from the injection chamber when the microfluidic chip is centrifuged;

[0010] The diluent inlet cavity is used to store the diluent entering the microfluidic chip;

[0011] The diluent quantitative chamber is in communication with the diluent inlet chamber, so as to quantitatively measure the diluent from the diluent inlet chamber when the microfluidic chip is centrifuged;

[0012] The mixing chamber is communicated with the first sample quantitative chamber and the diluent quantitative chamber respectively, so as to receive and mix the sample entering from the first sample quantitative chamber and the diluent entering from the diluent quantitative chamber when the microfluidic chip is centrifuged;

[0013] The distribution chamber is communicated with the mixing chamber and the reaction detection chamber respectively, so as to receive a mixed solution formed by mixing the sample and the diluent from the mixing chamber when the microfluidic chip is centrifuged, and distribute the mixed solution to the reaction detection chamber;

[0014] The reaction detection chamber is used for the reagent to react with the mixed solution to form a sample;

[0015] The first overflow chamber is communicated with the diluent quantitative chamber and the distribution chamber respectively, so as to collect the diluent overflowing from the diluent quantitative chamber and the mixed liquid overflowing from the distribution chamber;

[0016] The second overflow chamber is communicated with the first sample quantification chamber to collect the sample overflowing from the first sample quantification chamber.

[0017] As an embodiment, the non-circular chip body is further formed with a sample determination cavity, which is communicated with the second overflow cavity, so as to allow the optical detection component to detect whether there is a sample overflowing from the first sample quantification cavity;

[0018] The distance between the sample determination chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip is the same as the distance between the reaction detection chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip.

[0019] As an embodiment, the non-circular chip body is further formed with a diluent determination chamber, which is communicated with the first overflow chamber, so as to allow an optical detection component to detect whether there is diluent overflowing from the diluent quantitative chamber;

[0020] The distance between the diluent determination chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip is the same as the distance between the reaction detection chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip.

[0021] As an embodiment, the distance from the diluent judgment chamber to the central axis of rotation of the centrifugal rotation of the microfluidic chip is greater than the distance from the first overflow chamber to the central axis of rotation of the centrifugal rotation of the microfluidic chip.

[0022] As an embodiment, the sample judgment chamber and the diluent judgment chamber are respectively located on both sides of the reaction detection chamber pair along the direction of centrifugal rotation of the microfluidic chip.

[0023] As an embodiment, the non-circular chip body is further formed with a first channel and a second channel, the two ends of the first channel are respectively connected to the distribution chamber and the first overflow chamber, the two ends of the second channel are respectively connected to the distribution chamber and the reaction detection chamber, and the width of the first channel in the centrifugal rotation direction of the microfluidic chip is equal to the width of the second channel in the centrifugal rotation direction of the microfluidic chip.

[0024] In one embodiment, the non-circular chip body has a first plate surface and a second plate surface disposed opposite to each other, and the sample injection chamber, the first sample quantitative chamber, the diluent inlet chamber, the diluent quantitative chamber, the mixing chamber, the distribution chamber, the reaction detection chamber, the first overflow chamber, and the second overflow chamber are all recessed from the first plate surface toward the second plate surface, and are spaced apart from the second plate surface.

[0025] The microfluidic chip further includes a sealing film, which is attached to the first plate surface to cover at least the sample injection chamber, the first sample quantitative chamber, the diluent inlet chamber, the diluent quantitative chamber, the mixing chamber, the distribution chamber, the reaction detection chamber, the first overflow chamber, and the second overflow chamber;

[0026] The sealing film is provided with a sample injection hole at a position corresponding to the injection cavity. The sample injection hole is communicated with the injection cavity for injecting the sample into the injection cavity.

[0027] As an embodiment, the sealing film is provided with a diluent injection hole at a portion corresponding to the diluent inlet cavity, and the diluent injection hole is communicated with the diluent inlet cavity for injecting the diluent into the diluent inlet cavity; or,

[0028] A diluent bag is placed in the diluent inlet cavity.

[0029] As an embodiment, the non-circular chip body is further formed with a diluent overflow channel and a sample overflow channel, the diluent overflow channel is connected between the diluent quantitative chamber and the first overflow chamber, and the sample overflow channel is connected between the first sample quantitative chamber and the second overflow chamber;

[0030] The sealing film is further penetrated by a first vent hole, a second vent hole and a third vent hole. The first vent hole is communicated with the diluent overflow channel, the second vent hole is communicated with the sample overflow channel, and the third vent hole is communicated with the mixing chamber.

[0031] As an embodiment, the non-circular chip body is further formed with a second sample quantification cavity, a sample quantification channel and a sample drainage capillary, and the two ends of the sample quantification channel are respectively connected to the first sample quantification cavity and the second sample quantification cavity;

[0032] The distance between the sample quantification channel and the central axis of rotation of the centrifugal rotation of the microfluidic chip is greater than the distance between the first sample quantification chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip, and is less than the distance between the second sample quantification chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip;

[0033] The two ends of the sample drainage capillary are respectively connected to the sample quantitative channel and the mixing chamber, and the sample drainage capillary has a first bending portion, and the distance between the first bending portion and the central axis of rotation of the centrifugal rotation of the microfluidic chip is smaller than the distance between the first sample quantitative chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip.

[0034] As an embodiment, the non-circular chip body is further formed with a mixed liquid drainage capillary and an initial liquid cavity;

[0035] The two ends of the mixed liquid drainage capillary are respectively connected to the mixing chamber and the distribution chamber, and the mixed liquid drainage capillary has a second bent portion, and the distance between the second bent portion and the central axis of rotation of the centrifugal rotation of the microfluidic chip is smaller than the distance between the mixing chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip;

[0036] The initial liquid chamber is in communication with one end of the distribution chamber close to the mixed liquid drainage capillary, so as to at least collect the initial liquid entering the distribution chamber from the mixed liquid drainage capillary;

[0037] The distance between the initial liquid chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip is smaller than the distance between the reaction detection chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip.

[0038] As an embodiment, the volume of the initial liquid chamber is smaller than the volume of the reaction detection chamber; and / or,

[0039] The non-circular chip body also forms a second channel and a third channel, the two ends of the second channel are respectively connected to the distribution chamber and the reaction detection chamber, the two ends of the third channel are respectively connected to the distribution chamber and the initial liquid chamber, and the width of the third channel in the centrifugal rotation direction of the microfluidic chip is equal to the width of the second channel in the centrifugal rotation direction of the microfluidic chip.

[0040] In one embodiment, the non-circular chip body includes a first edge and a second edge, the first edge and the second edge are spaced apart and arranged opposite to each other, the first edge is provided at an end of the microfluidic chip close to the central axis of rotation of the microfluidic chip centrifugally, and the second edge is provided at an end of the microfluidic chip away from the central axis of rotation of the microfluidic chip centrifugally;

[0041] The diluent inlet chamber, the diluent quantitative chamber, the mixing chamber, the distribution chamber, and the first overflow chamber are sequentially arranged between the first edge and the second edge;

[0042] The injection chamber, the first sample quantitative chamber, the mixing chamber, the distribution chamber, and the second overflow chamber are sequentially arranged between the first edge and the second edge.

[0043] As an embodiment, the length of the second edge is greater than the length of the first edge.

[0044] As an embodiment, the first edge and the second edge are two arc-shaped edges with the same center; or,

[0045] The first edge and the second edge are two straight edges parallel to each other.

[0046] As an embodiment, the non-circular chip body further includes a third edge and a fourth edge;

[0047] The third edge is spaced apart from the fourth edge and is arranged opposite to each other. The third edge extends from one end of the first edge to one end of the second edge, and the fourth edge extends from the other end of the first edge to the other end of the second edge.

[0048] The diluent inlet chamber, the diluent quantitative chamber, one end of the mixing chamber, one end of the distribution chamber, and the first overflow chamber are arranged in sequence along the third edge;

[0049] The injection chamber, the first sample quantitative chamber, and the second overflow chamber are arranged in sequence along the fourth edge.

[0050] As an embodiment, the angle formed by the third edge and the fourth edge is greater than 0° and less than or equal to 90°.

[0051] As an embodiment, the angle formed by the third edge and the fourth edge is 60°±15°.

[0052] As an embodiment, the number of the reaction detection cavities formed by the non-circular chip body is less than or equal to six.

[0053] As an embodiment, the number of the reaction detection cavities formed by the non-circular chip body is two, three, four or five.

[0054] A second object of the present application is to provide a microfluidic chip comprising a non-circular chip body, wherein the non-circular chip body is formed with a sample injection chamber, a first sample quantification chamber, a diluent inlet chamber, a diluent quantification chamber, a mixing chamber, a distribution chamber, a reaction detection chamber, a third overflow chamber, and a fourth overflow chamber;

[0055] The first sample quantification chamber is in communication with the injection chamber, so as to quantify the sample from the injection chamber when the microfluidic chip is centrifuged;

[0056] The diluent quantitative chamber is in communication with the diluent inlet chamber, so as to quantitatively measure the diluent from the diluent inlet chamber when the microfluidic chip is centrifuged;

[0057] The mixing chamber is communicated with the first sample quantitative chamber and the diluent quantitative chamber respectively, so as to receive and mix the sample entering from the first sample quantitative chamber and the diluent entering from the diluent quantitative chamber when the microfluidic chip is centrifuged;

[0058] The distribution chamber is communicated with the mixing chamber and the reaction detection chamber respectively, so as to receive a mixed solution formed by mixing the sample and the diluent from the mixing chamber when the microfluidic chip is centrifuged, and distribute the mixed solution to the reaction detection chamber;

[0059] The reaction detection chamber is used for the reagent to react with the mixed solution to form a sample;

[0060] The third overflow chamber is in communication with the diluent quantitative chamber, and is used to collect the diluent overflowing from the diluent quantitative chamber;

[0061] The fourth overflow chamber is communicated with the first sample quantitative chamber and the distribution chamber respectively, so as to collect the sample overflowing from the first sample quantitative chamber and the mixed liquid overflowing from the distribution chamber.

[0062] The third object of the present application is to provide a microfluidic analysis system, which includes a turntable, an optical detection component, a rotation drive mechanism and the above-mentioned microfluidic chip;

[0063] The turntable is formed with a plurality of accommodating positions distributed in sequence along the circumferential direction, each of the accommodating positions is used to accommodate one of the microfluidic chips or a counterweight component with the same outer contour and the same weight as the microfluidic chip;

[0064] The optical detection assembly includes a light emitting element and a light receiving element, wherein the light emitting element is disposed above the turntable and is used to at least irradiate light toward the sample in the reaction detection chamber;

[0065] The light receiving element is provided below the turntable and directly below the light emitting element, so as to receive the light emitted by the light emitting element through the microfluidic chip;

[0066] The rotary drive mechanism is used to drive the turntable to drive the microfluidic chip to rotate, so as to respectively achieve: quantification of the sample and the diluent, mixing of the sample and the diluent, distribution of the mixed solution, and rotation of the reaction detection chamber to directly below the light receiving element.

[0067] The microfluidic chip and microfluidic analysis system provided in the present application implement sample loading on the microfluidic chip through a sample injection chamber, implement sample loading on the microfluidic chip through a diluent inlet chamber, quantify the sample from the sample injection chamber through a first sample quantification chamber, quantify the diluent from the diluent inlet chamber through a diluent quantification chamber, receive and mix the sample entering from the first sample quantification chamber and the diluent entering from the diluent quantification chamber through a mixing chamber, receive a mixed liquid formed by mixing the sample and diluent entering from the mixing chamber through a distribution chamber and distribute the mixed liquid to a reaction detection chamber, and collect and quantify excess diluent and sample through a first overflow chamber and a second overflow chamber. Therefore, after the sample and diluent are added to the microfluidic chip, the microfluidic chip can automatically complete the quantification, mixing, distribution and detection of the sample and diluent through rotation, and the operation is simple and the quantification is accurate. Since the sample injection chamber, the first sample quantitative chamber, the diluent inlet chamber, the diluent quantitative chamber, the mixing chamber, the distribution chamber, the reaction detection chamber, the first overflow chamber, and the second overflow chamber are all formed in the non-circular chip body, that is, the microfluidic chip is not a completely circular chip, it is beneficial to reduce the number of reaction detection chambers and to reduce the waste of samples, diluents, and reagents during the review. In addition, since the first overflow chamber is used to collect the diluent overflowing from the diluent quantitative chamber and the mixed liquid overflowing from the distribution chamber, it is equivalent to combining the overflow chamber for diluent quantitative flow and the overflow chamber for mixed liquid into one, thereby reducing the number of overflow chambers, and further simplifying the structure of the microfluidic chip and improving the structural compactness of the microfluidic chip. Ultimately, it is beneficial to the miniaturization design of the microfluidic chip, thereby further reducing the material cost of the microfluidic chip, and further reducing the cost of review using the microfluidic chip. BRIEF DESCRIPTION OF THE DRAWINGS

[0068] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0069] FIG1 is a schematic diagram of a top view of the microfluidic chip provided in Example 1 of the present application;

[0070] FIG2 is a schematic diagram of a state after a sample and a diluent are added to a microfluidic chip according to Example 1 of the present application;

[0071] FIG3 is a schematic diagram of the microfluidic chip provided in Example 1 of the present application after the first centrifugal rotation to complete the dilution and sample quantification;

[0072] FIG4 is a schematic diagram of the state of the microfluidic chip provided in Example 1 of the present application, wherein the sample and the diluent are drained under the capillary action after the first centrifugal rotation stops;

[0073] FIG5 is a schematic diagram of the microfluidic chip provided in Example 1 of the present application after the second centrifugal rotation to complete the mixing of the diluent and the sample;

[0074] FIG6 is a schematic diagram of the state of the microfluidic chip provided in Example 1 of the present application, wherein the mixed liquid is drained under capillary action after the second centrifugal rotation is stopped;

[0075] FIG7 is a schematic diagram of the state of the microfluidic chip provided in Example 1 of the present application after the third centrifugal rotation to complete the distribution of the mixed liquid;

[0076] FIG8 is a schematic diagram of the main structure of the microfluidic chip provided in Example 1 of the present application;

[0077] FIG9 is a schematic diagram of the distribution of multiple microfluidic chips on a turntable provided in Example 1 of the present application;

[0078] FIG10 is a schematic diagram showing the positions of the microfluidic chip and the optical detection component provided in Example 1 of the present application;

[0079] FIG11 is a schematic diagram of the composition of the microfluidic analysis system provided in Example 1 of the present application;

[0080] FIG12 is a schematic diagram of a top view of the microfluidic chip provided in Example 2 of the present application;

[0081] FIG13 is a schematic diagram of the top view of the microfluidic chip provided in Example 3 of the present application.

[0082] Explanation of the accompanying symbols: 100, microfluidic chip; 110, non-circular chip body; 111, sample injection chamber; 112, first sample quantitative chamber; 113, diluent inlet chamber; 114, diluent quantitative chamber; 115, mixing chamber; 116, distribution chamber; 117, reaction detection chamber; 118, first overflow chamber; 119, second overflow chamber; 101, sample judgment chamber; 102, diluent judgment chamber; 103, diluent overflow channel; 104, sample overflow channel; 105, second sample quantitative chamber; 106, sample quantitative pipeline; 107, sample drainage capillary; 1071, first bending portion; 108, diluent drainage Flow capillary; 1081, third bend; 109, mixed liquid drainage capillary; 1091, second bend; 1001, initial liquid chamber; 1002, first channel; 1003, second channel; 1004, third channel; 1005, first edge; 1006, second edge; 1007, third edge; 1008, fourth edge; 1009, first plate surface; 1010, second plate surface; 1011, third overflow chamber; 1012, fourth overflow chamber; 120, sealing membrane; 121, sample injection hole; 122, diluent injection hole; 123, first vent hole; 124, second vent hole; 125, third vent hole; 200, turntable; 300, optical detection component; 310, light emitting element; 320, light receiving element; 400, rotation drive mechanism; 500, controller; MN, rotation center axis. DETAILED DESCRIPTION

[0083] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0084] It should be noted that all directional indications in the embodiments of the present application (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship and movement status between the components in a certain specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0085] It should also be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it may be directly connected to the other element or indirectly connected to the other element through an intervening element.

[0086] In addition, the descriptions of "first", "second", etc. in this application are for descriptive purposes only and should not be understood as indicating or implying their relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined as "first" or "second" may explicitly or implicitly include at least one of such features. In addition, the technical solutions between the various embodiments can be combined with each other, but this must be based on the fact that they can be implemented by ordinary technicians in this field. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by this application.

[0087] Example 1:

[0088] As shown in Figures 1, 8 and 9, the microfluidic chip 100 provided in Example 1 of the present application includes a non-circular chip body 110, and the non-circular chip body 110 is specifically a chip structure with a non-complete circumference, that is, the outer edge of the non-circular chip body 110 is not circular. The microfluidic chip 100 as a whole is a non-circular structure, that is, the microfluidic chip 100 is not a full circle. Compared with a full-circular chip, the material cost of the microfluidic chip 100 can be reduced. When used for the detection of a small number of detection items (such as the review of some detection items), the cost and the consumption of samples, diluents and reagents can be reduced. When used for the detection of a large number of detection items, more than two microfluidic chips 100 can be used for combined detection to meet the detection needs of different numbers of detection items, and the flexibility of use is high.

[0089] 1, 2 and 8, as an embodiment, the non-circular chip body 110 is formed with a sampling chamber 111, a first sample quantitative chamber 112, a diluent inlet chamber 113, a diluent quantitative chamber 114, a mixing chamber 115, a distribution chamber 116 and a reaction detection chamber 117. The sampling chamber 111 is used to store the sample entering the microfluidic chip 100; the first sample quantitative chamber 112 is connected to the sampling chamber 111, so as to quantify the sample from the sampling chamber 111 when the microfluidic chip 100 is centrifuged; the diluent inlet chamber 113 is used to store the diluent entering the microfluidic chip 100; the diluent quantitative chamber 114 is connected to the diluent inlet chamber 113, so as to quantify the diluent from the diluent inlet chamber 113 when the microfluidic chip 100 is centrifuged; the mixing chamber 115 is connected to the first sample quantitative chamber 112, the diluent quantitative chamber 113 and the diluent quantitative chamber 114 respectively. The liquid quantitative chamber 114 is connected to receive and mix the sample entering from the first sample quantitative chamber 112 and the diluent entering from the diluent quantitative chamber 114 when the microfluidic chip 100 is centrifuged; the distribution chamber 116 is connected to the mixing chamber 115 and the reaction detection chamber 117 respectively to receive the mixed liquid formed by the sample and the diluent entering from the mixing chamber 115 when the microfluidic chip 100 is centrifuged, and distribute the mixed liquid to the reaction detection chamber 117; the reaction detection chamber 117 is used for the reagent to react with the mixed liquid to form a test sample. In this embodiment, after the sample and diluent are added to the microfluidic chip 100, the rotation of the microfluidic chip 100 can automatically complete the quantification, mixing, distribution and detection of the sample and diluent. The operation is simple and convenient, and the quantification is accurate. Since the sample injection chamber 111, the first sample quantitative chamber 112, the diluent inlet chamber 113, the diluent quantitative chamber 114, the mixing chamber 115, the distribution chamber 116 and the reaction detection chamber 117 are all formed in the non-circular chip body 110, it is beneficial to reduce the number of reaction detection chambers 117 on the microfluidic chip 100, which is beneficial to reduce the waste of samples, diluents and reagents during re-examination.

[0090] 1 , 3 , and 8 , as an embodiment, the non-circular chip body 110 further includes a first overflow cavity 118 , which is in communication with the diluent quantification cavity 114 and is configured to collect diluent overflowing from the diluent quantification cavity 114 . The first overflow cavity 118 can be used to collect excess diluent after quantification, thereby ensuring sufficient diluent while preventing excess diluent, thereby effectively ensuring the accuracy of diluent quantification.

[0091] 1 , 3 and 7 , as an embodiment, the first overflow chamber 118 is also connected to the distribution chamber 116 to collect the mixed liquid overflowing from the distribution chamber 116. In this embodiment, the first overflow chamber 118 is used to collect both the diluent overflowing from the diluent quantitative chamber 114 and the mixed liquid overflowing from the distribution chamber 116, which is equivalent to combining the overflow chamber for the quantitative diluent and the overflow chamber for the mixed liquid into one, thereby reducing the number of overflow chambers, simplifying the structure of the microfluidic chip 100 and improving the compactness of the structure of the microfluidic chip 100, and ultimately facilitating the miniaturization design of the microfluidic chip 100, thereby further reducing the material cost of the microfluidic chip 100, and further reducing the cost of review using the microfluidic chip 100.

[0092] 1 , 3 , and 8 , as an embodiment, the non-circular chip body 110 further comprises a second overflow chamber 119, which is in communication with the first sample quantification chamber 112 and is used to collect sample overflowing from the first sample quantification chamber 112. The second overflow chamber 119 is primarily used to collect excess sample after quantification, thereby ensuring sufficient sample and preventing excess sample, thereby effectively ensuring accurate sample quantification.

[0093] As shown in Figures 1, 3, and 8, as an embodiment, the non-circular chip body 110 further includes a sample determination chamber 101. The sample determination chamber 101 is connected to the second overflow chamber 119 to allow the optical detection assembly 300 to detect whether there is sample overflowing from the first sample quantification chamber 112. When the optical detection assembly 300 detects the presence of sample in the sample determination chamber 101, it indicates that the first sample quantification chamber 112 is full, thereby determining that the sample volume is sufficient; otherwise, it is determined that the sample volume is insufficient. The provision of the sample determination chamber 101 can further ensure the accuracy and reliability of sample quantification.

[0094] 1 , 3 , and 10 , as one embodiment, the distance between the sample judgment chamber 101 and the central axis MN of centrifugal rotation of the microfluidic chip 100 is the same as the distance between the reaction detection chamber 117 and the central axis MN of centrifugal rotation of the microfluidic chip 100. In this embodiment, the distance between the sample judgment chamber 101 and the central axis MN of centrifugal rotation of the microfluidic chip 100 is the same as the distance between the reaction detection chamber 117 and the central axis MN of centrifugal rotation of the microfluidic chip 100. This allows the sample judgment chamber 101 and the reaction detection chamber 117 to sequentially pass through the same position during rotation of the microfluidic chip 100. This facilitates the determination of the presence of a sample in the sample judgment chamber 101 and the detection of a sample in the reaction detection chamber 117 to share the optical detection assembly 300, thereby simplifying the structure of the microfluidic system. Of course, in specific applications, as an alternative implementation scheme, the presence or absence of the sample in the sample judgment chamber 101 can also be detected by a separate independent sensor, without sharing the optical detection component 300 with the reaction detection chamber 117. In this way, the distance from the sample judgment chamber 101 to the rotation center axis MN of the microfluidic chip 100 and the distance from the reaction detection chamber 117 to the rotation center axis MN of the microfluidic chip 100 may be different.

[0095] As shown in Figures 1, 3, and 8, as an embodiment, the non-circular chip body 110 further includes a diluent determination chamber 102. The diluent determination chamber 102 is connected to the first overflow chamber 118 to allow the optical detection assembly 300 to detect whether diluent has overflowed from the diluent quantification chamber 114. When the optical detection assembly 300 detects the presence of diluent in the diluent determination chamber 102, it indicates that the diluent quantification chamber 114 is full, thereby determining that the amount of diluent is sufficient. Otherwise, it is determined that the amount of diluent is insufficient. The provision of the diluent determination chamber 102 can further ensure the accuracy and reliability of diluent quantification.

[0096] 1 , 3 , and 10 , as an embodiment, the distance between the diluent judgment chamber 102 and the central axis MN of centrifugal rotation of the microfluidic chip 100 is the same as the distance between the reaction detection chamber 117 and the central axis MN of centrifugal rotation of the microfluidic chip 100. In this embodiment, the distance between the diluent judgment chamber 102 and the central axis MN of centrifugal rotation of the microfluidic chip 100 is the same as the distance between the reaction detection chamber 117 and the central axis MN of centrifugal rotation of the microfluidic chip 100. This allows the diluent judgment chamber 102 and the reaction detection chamber 117 to sequentially pass through the same position during rotation of the microfluidic chip 100, thereby facilitating the determination of the presence of diluent in the diluent judgment chamber 102 and the detection of the sample in the reaction detection chamber 117 to share the optical detection assembly 300, thereby simplifying the structure of the microfluidic system. Of course, in specific applications, as an alternative implementation scheme, the presence or absence of diluent in the diluent judgment chamber 102 can also be detected by a separate independent sensor, without sharing the optical detection component 300 with the reaction detection chamber 117. In this way, the distance from the diluent judgment chamber 102 to the rotation center axis MN of the microfluidic chip 100 and the distance from the reaction detection chamber 117 to the rotation center axis MN of the microfluidic chip 100 can be different.

[0097] As an embodiment, the diluent judgment chamber 102, the reaction detection chamber 117 and the sample judgment chamber 101 are distributed in sequence along the same arc trajectory. In this way, when the microfluidic chip 100 rotates, the diluent judgment chamber 102, the reaction detection chamber 117 and the sample judgment chamber 101 can pass through the same position in sequence, thereby facilitating the judgment of the presence of diluent in the diluent judgment chamber 102, the judgment of the presence of sample in the sample judgment chamber 101 and the detection of the sample in the reaction detection chamber 117 to share the optical detection component 300.

[0098] 1 , 2 and 10 , as an embodiment, the distance between the diluent determination chamber 102 and the central axis MN of centrifugal rotation of the microfluidic chip 100 is greater than the distance between the first overflow chamber 118 and the central axis MN of centrifugal rotation of the microfluidic chip 100. This helps ensure that a quantitative amount of excess diluent enters the diluent determination chamber 102 first under centrifugal action, and enters the first overflow chamber 118 only after the diluent determination chamber 102 is full.

[0099] 1 , 2 and 10 , as an embodiment, the distance between the sample judgment chamber 101 and the central axis MN of centrifugal rotation of the microfluidic chip 100 is greater than the distance between the second overflow chamber 119 and the central axis MN of centrifugal rotation of the microfluidic chip 100. This helps ensure that the quantitative excess sample will preferentially enter the sample judgment chamber 101 under the action of centrifugation, and will enter the second overflow chamber 119 only after the diluent judgment chamber 102 is full.

[0100] 1 , 2 and 3 , as an embodiment, the sample judgment chamber 101 and the diluent judgment chamber 102 are respectively located on both sides of the pair of reaction detection chambers 117 along the direction of centrifugal rotation of the microfluidic chip 100, that is, the reaction detection chamber 117 is located between the sample judgment chamber 101 and the diluent along the circumference of the microfluidic chip 100. This arrangement facilitates the sample-related chambers and the diluent-related chambers to be respectively concentrated on both sides of the circumference of the microfluidic chip 100, thereby improving the structural compactness of the microfluidic chip 100.

[0101] As shown in Figures 1, 3, 4, and 8, as an embodiment, the non-circular chip body 110 further forms a first channel 1002 and a second channel 1003. The two ends of the first channel 1002 are respectively connected to the distribution chamber 116 and the first overflow chamber 118, and the two ends of the second channel 1003 are respectively connected to the distribution chamber 116 and the reaction detection chamber 117. The width L1 of the first channel 1002 in the centrifugal rotation direction of the microfluidic chip 100 is equal to the width L2 of the second channel 1003 in the centrifugal rotation direction of the microfluidic chip 100. In this embodiment, the first channel 1002 and the second channel 1003 are designed to be equal in width without being differentiated, which helps to reduce the manufacturing difficulty of the microfluidic chip 100.

[0102] As shown in Figures 1, 3, and 8, as one embodiment, the non-circular chip body 110 further comprises a diluent overflow channel 103, which communicates between the diluent quantitative chamber 114 and the first overflow chamber 118. The diluent overflow channel 103 is also connected to the diluent determination chamber 102. The diluent overflow channel 103 is primarily used to direct the diluent overflowing from the diluent quantitative chamber 114 to the diluent determination chamber 102 and the first overflow chamber 118.

[0103] As an embodiment, one end of the diluent overflow channel 103 is connected to the end of the diluent quantitative chamber 114 close to the rotation center axis MN of the microfluidic chip 100, so that the diluent will enter the diluent overflow channel 103 under the action of centrifugation only after filling the diluent quantitative chamber 114.

[0104] As shown in Figures 1, 3, and 8, as one embodiment, the non-circular chip body 110 further comprises a sample overflow channel 104, which connects the first sample quantification chamber 112 and the second overflow chamber 119. The sample overflow channel 104 also connects to the sample determination chamber 101. The sample overflow channel 104 is primarily used to direct sample overflow from the first sample quantification chamber 112 to the sample determination chamber 101 and the second overflow chamber 119.

[0105] As an embodiment, one end of the sample overflow channel 104 is connected to the end of the first sample quantitative chamber 112 close to the rotation center axis MN of the microfluidic chip 100. In this way, the sample will enter the sample overflow channel 104 under centrifugal action only after filling the first sample quantitative chamber 112.

[0106] As shown in Figures 1, 3, 8, and 10, as an embodiment, the non-circular chip body 110 further includes a second sample quantification chamber 105 and a sample quantification channel 106. The two ends of the sample quantification channel 106 are connected to the first sample quantification chamber 112 and the second sample quantification chamber 105, respectively. The distance between the sample quantification channel 106 and the central axis MN of centrifugal rotation of the microfluidic chip 100 is greater than the distance between the first sample quantification chamber 112 and the central axis MN of centrifugal rotation of the microfluidic chip 100, and less than the distance between the second sample quantification chamber 105 and the central axis MN of centrifugal rotation of the microfluidic chip 100. The provision of the second sample quantification chamber 105 is primarily used to meet the requirement of centrifugal stratification of some samples before testing. For example, when the sample is a whole blood sample, after the sample is centrifuged and quantified, the plasma will be concentrated in the first sample quantification chamber 112, and the red blood cells will be concentrated in the second sample quantification chamber 105.

[0107] 3, 4, and 8, as one embodiment, the non-circular chip body 110 further includes a sample drainage capillary 107, the two ends of which are connected to the sample quantification channel 106 and the mixing chamber 115, respectively. The provision of the sample drainage capillary 107 is primarily used to ensure that sample quantification and mixing of the sample in the mixing chamber 115 can be performed separately during the two centrifugal rotations of the microfluidic chip 100, thereby ensuring accurate sample quantification.

[0108] 3 , 4 , 8 , and 10 , as one embodiment, the sample drainage capillary 107 has a first bend 1071 . The distance between the first bend 1071 and the central axis MN of centrifugal rotation of the microfluidic chip 100 is less than the distance between the first sample quantification chamber 112 and the central axis MN of centrifugal rotation of the microfluidic chip 100. In this embodiment, the distance between the first bend 1071 and the central axis MN of rotation of the microfluidic chip 100 is less than the distance between the first sample quantification chamber 112 and the central axis MN of rotation of the microfluidic chip 100. This helps ensure that during the centrifugal rotation phase of sample quantification, sample does not enter the mixing chamber 115 from the sample drainage capillary 107. During the sample quantification and stop phase, the sample in the sample drainage capillary 107 fills the sample drainage capillary 107 under capillary action. During the centrifugal rotation phase of mixing the sample with the diluent, the sample in the sample drainage capillary 107 flows into the mixing chamber 115 under the action of a siphon.

[0109] As an embodiment, one end of the sample drainage capillary 107 is connected to the end of the sample quantitative pipeline 106 close to the first sample quantitative chamber 112, and the other end of the sample drainage capillary 107 is connected to the end of the mixing chamber 115 close to the rotation center axis MN of the microfluidic chip 100.

[0110] As an implementation manner, the first bending portion 1071 is bent in an arc shape.

[0111] 3 , 4 and 8 , as an embodiment, the non-circular chip body 110 is further formed with a diluent drainage capillary 108 , the two ends of which are respectively connected to the diluent quantitative chamber 114 and the mixing chamber 115 . The diluent drainage capillary 108 is mainly used to ensure that the quantitative amount of the diluent and the mixing of the diluent into the mixing chamber 115 can be carried out separately in the two centrifugal rotations of the microfluidic chip 100 , thereby facilitating the quantitative accuracy of the diluent.

[0112] 3, 4, 8, and 10, as an embodiment, the diluent drainage capillary 108 has a third bend 1081, and the distance between the third bend 1081 and the central axis MN of centrifugal rotation of the microfluidic chip 100 is less than the distance between the diluent quantitative chamber 114 and the central axis MN of centrifugal rotation of the microfluidic chip 100. In this embodiment, the distance between the third bend 1081 and the central axis MN of rotation of the microfluidic chip 100 is less than the distance between the diluent quantitative chamber 114 and the central axis MN of rotation of the microfluidic chip 100, which helps to ensure that during the centrifugal rotation stage of the quantitative diluent, the diluent does not enter the mixing chamber 115 from the diluent drainage capillary 108; during the quantitative dilution completion and stop stage, the diluent in the diluent drainage capillary 108 fills the diluent drainage capillary 108 under capillary action; during the mixing centrifugal rotation stage, the diluent in the diluent drainage capillary 108 flows into the mixing chamber 115 under the action of siphon. Specifically, the quantification of the diluent and the quantification of the sample are performed in the same centrifugal rotation stage of the microfluidic chip 100 , and the diluent and the sample enter the mixing chamber 115 in the same centrifugal rotation stage of the microfluidic chip 100 .

[0113] As an embodiment, one end of the diluent drainage capillary 108 is connected to the end of the diluent quantitative chamber 114 away from the rotation center axis MN of the microfluidic chip 100, and the other end of the diluent drainage capillary 108 is connected to the end of the mixing chamber 115 close to the rotation center axis MN of the microfluidic chip 100.

[0114] As an implementation manner, the third bending portion 1081 is bent in an arc shape.

[0115] 5 to 8 , as an embodiment, the non-circular chip body 110 further comprises a mixed liquid drainage capillary 109 and an initial liquid chamber 1001. The two ends of the mixed liquid drainage capillary 109 are connected to the mixing chamber 115 and the distribution chamber 116, respectively. The initial liquid chamber 1001 is connected to the distribution chamber 116 near one end of the mixed liquid drainage capillary 109, so as to at least collect the initial liquid that enters the distribution chamber 116 from the mixed liquid drainage capillary 109. The provision of the mixed liquid drainage capillary 109 is primarily used to ensure that the mixing of the diluent and the sample and the distribution of the mixed liquid into the distribution chamber 116 can be performed separately during the two centrifugal rotations of the microfluidic chip 100. This helps ensure that the diluent and the sample are fully mixed before entering the distribution chamber 116, thereby facilitating the accuracy of the sample detection in the reaction detection chamber 117. The initial liquid chamber 1001 is used to collect the unmixed liquid from the mixed liquid drainage capillary 109 , which helps to ensure the accuracy of the sample detection in the reaction detection chamber 117 .

[0116] 7 , 8 , and 10 , as an embodiment, the distance between the initial liquid chamber 1001 and the central axis MN of rotation of the microfluidic chip 100 during centrifugal rotation is less than the distance between the reaction and detection chamber 117 and the central axis MN of rotation of the microfluidic chip 100 during centrifugal rotation. In this embodiment, the distance between the initial liquid chamber 1001 and the central axis MN of rotation of the microfluidic chip 100 is less than the distance between the reaction and detection chamber 117 and the central axis MN of rotation of the microfluidic chip 100. This helps ensure that when the microfluidic chip 100 is centrifuged, the liquid that enters the distribution chamber 116 from the mixed liquid drainage capillary 109 will preferentially fill the initial liquid chamber 1001, thereby allowing unmixed liquid to preferentially enter the initial liquid chamber 1001.

[0117] As an embodiment, the reaction detection chamber 117 is arranged between the initial liquid chamber 1001 and the first overflow chamber 118 along the circumferential direction of the centrifugal rotation of the microfluidic chip 100. In this way, the initial liquid chamber 1001 and the first overflow chamber 118 can be arranged close to the two ends of the distribution chamber 116, respectively, which is conducive to ensuring that the liquid from the mixed liquid drainage capillary 109 enters the distribution chamber 116, first fills the initial liquid chamber 1001, then fills the reaction detection chamber 117, and finally enters the first overflow chamber 118.

[0118] 5 to 8 , as one embodiment, the mixed liquid drainage capillary 109 has a second bend 1091. The distance between the second bend 1091 and the central axis MN of centrifugal rotation of the microfluidic chip 100 is less than the distance between the mixing chamber 115 and the central axis MN of centrifugal rotation of the microfluidic chip 100. In this embodiment, the distance between the second bend 1091 and the central axis MN of rotation of the microfluidic chip 100 is less than the distance between the mixing chamber 115 and the central axis MN of rotation of the microfluidic chip 100. This helps ensure that during the centrifugal rotation stage of mixing the diluent and sample, the sample and diluent do not flow from the mixed liquid drainage capillary 109 into the distribution chamber 116. During the mixing completion and stop stage, the mixed liquid in the mixed liquid drainage capillary 109 fills the mixed liquid drainage capillary 109 under capillary action. During the centrifugal rotation stage of mixed liquid distribution, the mixed liquid in the mixed liquid drainage capillary 109 flows into the distribution chamber 116 under the action of a siphon.

[0119] As an embodiment, one end of the mixed liquid drainage capillary 109 is connected to the end of the mixing chamber 115 away from the rotation center axis MN of the microfluidic chip 100, and the other end of the mixed liquid drainage capillary 109 is connected to the end of the distribution chamber 116 close to the rotation center axis MN of the microfluidic chip 100.

[0120] As an implementation manner, the second bending portion 1091 is bent in an arc shape.

[0121] 5 , 6 , and 7 , as one embodiment, the volume of the initial liquid chamber 1001 is smaller than that of the reaction and detection chamber 117. Since the initial liquid chamber 1001 is primarily used to collect a small amount of unmixed liquid in the mixed liquid drainage capillary 109, the initial liquid chamber 1001 is designed to be smaller than the reaction and detection chamber 117. This helps prevent excessive mixed liquid in the mixing chamber 115 from entering the initial liquid chamber 1001, resulting in waste of sample and diluent, and ensures that the subsequent reaction and detection chamber 117 can collect a sufficient amount of mixed liquid.

[0122] 3, 4, and 8, as an embodiment, the non-circular chip body 110 further comprises a second channel 1003 and a third channel 1004. The second channel 1003 is connected to the distribution chamber 116 and the reaction detection chamber 117 at both ends, and the third channel 1004 is connected to the distribution chamber 116 and the initial liquid chamber 1001 at both ends. The width L3 of the third channel 1004 in the centrifugal rotation direction of the microfluidic chip 100 is equal to the width L2 of the second channel 1003 in the centrifugal rotation direction of the microfluidic chip 100. The width L3 of the third channel 1004 in the centrifugal rotation direction of the microfluidic chip 100 is the circumferential width of the third channel 1004. The width L2 of the second channel 1003 in the centrifugal rotation direction of the microfluidic chip 100 is the circumferential width of the second channel 1003. In this embodiment, the second channel 1003 and the third channel 1004 are designed to be equal in width, without being differentiated, which helps reduce the manufacturing difficulty of the microfluidic chip 100.

[0123] As an embodiment, the reaction detection chamber 117 is loaded with a reagent, and the mixed solution reacts with the reagent to form a sample after entering the reaction detection chamber 117 .

[0124] In one embodiment, the reagent loaded into the reaction detection chamber 117 is a lyophilized pellet reagent. Lyophilized pellet reagents are produced using a freeze-drying method. Lyophilized pellet reagents are small in size and help extend the shelf life of the reagent. Using a smaller lyophilized pellet reagent allows for a larger sample loading capacity within the same sized reaction detection chamber 117, thereby improving detection sensitivity and efficiency.

[0125] 1 , 3 , and 8 , as one embodiment, the non-circular chip body 110 includes a first edge 1005 and a second edge 1006. The first edge 1005 and the second edge 1006 are spaced apart and arranged in opposition to each other. The first edge 1005 is located at an end of the microfluidic chip 100 that is closer to the central axis MN of centrifugal rotation of the microfluidic chip 100, and the second edge 1006 is located at an end of the microfluidic chip 100 that is farther from the central axis MN of centrifugal rotation of the microfluidic chip 100. The diluent inlet chamber 113, the diluent quantitative chamber 114, the mixing chamber 115, the dispensing chamber 116, and the first overflow chamber 118 are sequentially arranged between the first edge 1005 and the second edge 1006. The sample inlet chamber 111, the first sample quantitative chamber 112, the mixing chamber 115, the dispensing chamber 116, and the second overflow chamber 119 are sequentially arranged between the first edge 1005 and the second edge 1006. Specifically, the first edge 1005 is the inner edge of the non-circular chip body 110, and the second edge 1006 is the outer edge of the non-circular chip body 110. The diluent chamber 113, the diluent quantitative chamber 114, the mixing chamber 115, the distribution chamber 116, and the first overflow chamber 118 are arranged in sequence from the inner edge to the outer edge. The injection chamber 111, the first sample quantitative chamber 112, the mixing chamber 115, the distribution chamber 116, and the second overflow chamber 119 are also arranged in sequence from the inner edge to the outer edge. In this way, it is beneficial to ensure that the microfluidic chip When the sheet 100 is centrifugally rotated, the sample in the injection chamber 111 can enter the first sample quantitative chamber 112 and the second overflow chamber 119 under the action of centrifugation, the sample in the diluent chamber 113 can enter the diluent quantitative chamber 114 and the first overflow chamber 118 under the action of centrifugation, the sample in the first sample quantitative chamber 112 can enter the mixing chamber 115 under the action of centrifugation, the diluent in the diluent quantitative chamber 114 can enter the mixing chamber 115 under the action of centrifugation, and the mixed liquid in the mixing chamber 115 enters the distribution chamber 116 under the action of centrifugation.

[0126] As an embodiment, the second sample quantitative chamber 105 is arranged on the side of the first sample quantitative chamber 112 facing the second edge 1006, that is, the second sample quantitative chamber 105 is arranged between the first sample quantitative chamber 112 and the second edge 1006 along the direction from the first edge 1005 to the second edge 1006. In this way, it is beneficial to ensure that the larger mass part of the sample can preferentially enter the second sample quantitative chamber 105 under the action of centrifugation, thereby facilitating the centrifugal stratification of the sample. For example, the red blood cells in the whole blood sample enter the second sample quantitative chamber 105 under the action of centrifugation, and the plasma in the whole blood sample enters the first sample quantitative chamber 112 under the action of centrifugation.

[0127] As an embodiment, the reaction detection chamber 117 is arranged on the side of the distribution chamber 116 facing the second edge 1006, that is, the reaction detection chamber 117 is arranged between the distribution chamber 116 and the second edge 1006 along the direction of the first edge 1005 toward the second edge 1006. In this way, it is beneficial to ensure that the mixed liquid in the distribution chamber 116 can enter the reaction detection chamber 117 under the action of centrifugation.

[0128] 1 , 3 and 8 , as an embodiment, the non-circular chip body 110 further includes a third edge 1007 and a fourth edge 1008; the third edge 1007 and the fourth edge 1008 are spaced apart and arranged opposite to each other, the third edge 1007 extends from one end of the first edge 1005 to one end of the second edge 1006, and the fourth edge 1008 extends from the other end of the first edge 1005 to the other end of the second edge 1006; the diluent inlet chamber 113, the diluent quantitative chamber 114, one end of the mixing chamber 115, one end of the distribution chamber 116, and the first overflow chamber 118 are arranged in sequence along the third edge 1007; the sample injection chamber 111, the first sample quantitative chamber 112, and the second overflow chamber 119 are arranged in sequence along the fourth edge 1008. The third edge 1007 and the fourth edge 1008 are the two side edges of the non-circular chip body 110 in the circumferential direction. In this embodiment, the sample-related cavities are arranged along one side edge of the non-circular chip body 110 in the circumferential direction, and the diluent-related cavities are arranged along the other side edge of the non-circular chip body 110 in the circumferential direction, which is conducive to optimizing the layout of each cavity and improving the structural compactness of the microfluidic chip 100.

[0129] In one embodiment, the length of the second edge 1006 is greater than the length of the first edge 1005, that is, the outer edge of the microfluidic chip 100 is longer than the inner edge. The third edge 1007 and the fourth edge 1008 extend from the second edge 1006 to the first edge 1005 with a gradually decreasing spacing. This arrangement facilitates the close arrangement of adjacent microfluidic chips 100 when multiple microfluidic chips 100 are arranged along the circumference. Of course, in specific applications, as an alternative embodiment, the length of the second edge 1006 can also be equal to the length of the first edge 1005.

[0130] In one embodiment, the first edge 1005 and the second edge 1006 are two concentric arc-shaped edges. In this embodiment, the microfluidic chip 100 has a fan-shaped structure, wherein the first edge 1005 and the second edge 1006 are both arc-shaped and concentric, and the third edge 1007 and the fourth edge 1008 extend from the second edge 1006 to the first edge 1005 with a gradually decreasing distance therebetween.

[0131] 1 , 3 , and 8 , as an embodiment, the angle A formed by the third edge 1007 and the fourth edge 1008 is greater than 0° and less than or equal to 90°, that is, the central angle A of the non-circular chip body 110 is between 0° and 90°. By adopting the above-described layout of the cavities on the non-circular chip body 110, the central angle of the non-circular chip body 110 can be designed to be less than or equal to 90°, thereby making the volume of the non-circular chip body 110 relatively small, which helps reduce the cost and unnecessary waste of retesting using the microfluidic chip 100.

[0132] As an embodiment, the included angle A formed by the third edge 1007 and the fourth edge 1008 is 60°±15°, that is, the central angle A of the non-circular chip body 110 is between 60°±15°.

[0133] As an embodiment, the angle A formed by the third edge 1007 and the fourth edge 1008 is 60°, that is, the central angle A of the non-circular chip body 110 is 60°. In this way, six non-circular chip bodies 110 can be combined to form a complete circular chip structure.

[0134] As an embodiment, the number of reaction detection cavities 117 formed by the non-circular chip body 110 is less than or equal to six, so that the volume of a single microfluidic chip 100 can be relatively small, and the number of reaction detection cavities 117 on a single microfluidic chip 100 is also relatively small. When a single microfluidic chip 100 is used for re-inspection, the cost of the microfluidic chip 100 can be reduced, and the waste of samples, diluents and reagents can be reduced.

[0135] As an embodiment, the number of reaction detection cavities 117 formed by the non-circular chip body 110 is three. This can effectively meet the needs of retesting using a single microfluidic chip 100 and effectively avoid waste of samples, diluents, and reagents. Of course, in specific applications, the number of reaction detection cavities 117 formed by the non-circular chip body 110 can also be two, three, four, five, etc.

[0136] 1 , 3 and 8 , as an embodiment, the non-circular chip body 110 has a first plate surface 1009 and a second plate surface 1010 arranged in opposite directions, and the sampling chamber 111, the first sample quantitative chamber 112, the diluent inlet chamber 113, the diluent quantitative chamber 114, the mixing chamber 115, the distribution chamber 116, the reaction detection chamber 117, the first overflow chamber 118 and the second overflow chamber 119 are all recessed from the first plate surface 1009 toward the second plate surface 1010, and are all spaced apart from the second plate surface 1010, that is, the sampling chamber 111, the first sample quantitative chamber 112, the diluent inlet chamber 113, the diluent quantitative chamber 114, the mixing chamber 115, the distribution chamber 116, the reaction detection chamber 117, the first overflow chamber 118 and the second overflow chamber 119 are not arranged to pass through along the thickness direction of the non-circular chip body 110, that is, these chambers are all concave cavity structures similar to blind holes.

[0137] 1 , 3 , and 8 , as an embodiment, the microfluidic chip 100 further includes a sealing film 120, which is attached to the first plate surface 1009 to cover at least the sample injection chamber 111, the first sample quantitative chamber 112, the diluent inlet chamber 113, the diluent quantitative chamber 114, the mixing chamber 115, the dispensing chamber 116, the reaction and detection chamber 117, the first overflow chamber 118, and the second overflow chamber 119. The sealing film 120 is primarily used to seal and protect the sample injection chamber 111, the first sample quantitative chamber 112, the diluent inlet chamber 113, the diluent quantitative chamber 114, the mixing chamber 115, the dispensing chamber 116, the reaction and detection chamber 117, the first overflow chamber 118, and the second overflow chamber 119.

[0138] As an embodiment, the second sample quantification cavity 105 is also recessed from the first plate surface 1009 toward the second plate surface 1010 and is spaced apart from the second plate surface 1010. This means that the second sample quantification cavity 105 does not extend through the thickness of the non-circular chip body 110. Instead, the second sample quantification cavity 105 has a concave structure similar to a blind hole. The sealing film 120 also covers the second sample quantification cavity 105.

[0139] 1, 2, and 8, as one embodiment, the sealing film 120 is provided with a sample injection hole 121 at a position corresponding to the injection chamber 111. The sample injection hole 121 is connected to the injection chamber 111 for injecting the sample into the injection chamber 111. The sample to be tested can be injected into the injection chamber 111 through the sample injection hole 121 to achieve sample addition. Of course, the sample injection hole 121 can also be temporarily manufactured after the sample is injected into the injection chamber 111.

[0140] 1 , 2 , and 8 , as one embodiment, a diluent injection hole 122 is formed through the sealing film 120 at a location corresponding to the diluent inlet chamber 113. The diluent injection hole 122 communicates with the diluent inlet chamber 113 for injecting diluent into the diluent chamber 113. In this embodiment, the diluent is injected into the diluent chamber 113 through the diluent injection hole 122. Of course, in a specific application, as an alternative embodiment, a diluent bag may be placed in the diluent inlet chamber 113. When the diluent is needed, the diluent in the diluent bag is pressed or punctured to allow the diluent to flow into the diluent inlet chamber 113.

[0141] 1 , 2 and 8 , as an embodiment, the sealing film 120 is further provided with a first vent 123, which is in communication with the diluent overflow channel 103. Since the diluent overflow channel 103 is respectively in communication with the diluent quantitative chamber 114, the first overflow chamber 118 and the diluent judgment chamber 102, and the first overflow chamber 118 is in communication with the distribution chamber 116, when the diluent is quantitatively measured, the gas in the diluent quantitative chamber 114 and the gas in the diluent judgment chamber 102 can be discharged from the first vent 123; when the mixed liquid is distributed, the gas in the distribution chamber 116 and the reaction detection chamber 117 can also be discharged from the first vent 123. In this embodiment, the diluent quantitative chamber 114 and the distribution chamber 116 share a vent, which is conducive to reducing the number of vents, thereby simplifying the structure of the microfluidic chip 100.

[0142] 1 , 2 , and 8 , as an embodiment, the sealing film 120 is further provided with a second vent 124 extending therethrough, which communicates with the sample overflow channel 104. Since the sample overflow channel 104 is respectively connected to the first sample quantification chamber 112, the second overflow chamber 119, and the sample determination chamber 101, and the first sample quantification chamber 112 is in turn connected to the second sample quantification chamber 105, during sample quantification, gas within the first sample quantification chamber 112, the second sample quantification chamber 105, and the sample determination chamber 101 can be discharged through the second vent 124.

[0143] 1 , 5 and 8 , as an embodiment, the sealing film 120 is further provided with a third vent hole 125 , which is in communication with the mixing chamber 115 . When the sample and the diluent are mixed, the gas in the mixing chamber 115 can be discharged through the third vent hole 125 .

[0144] As an embodiment, the microfluidic chip 100 is used for detecting blood samples, that is, the sample added to the injection chamber 111 is a blood sample. Of course, in specific applications, the microfluidic chip 100 can also be used for detecting other samples, such as urine samples.

[0145] As an embodiment, the microfluidic chip 100 is used for the detection of whole blood samples, plasma samples or serum samples, that is, the microfluidic chip 100 can simultaneously meet the detection requirements of whole blood samples, plasma samples and serum samples, and has a wide range of applications.

[0146] 8, 10, and 11, the present embodiment further provides a microfluidic analysis system, which includes a turntable 200, an optical detection assembly 300, a rotation drive mechanism 400, and the above-mentioned microfluidic chip 100. The turntable 200 is used to load at least one microfluidic chip 100, the rotation drive mechanism 400 is used to drive the turntable 200 to rotate the microfluidic chip 100, and the optical detection assembly 300 is used to perform optical detection on the sample in the reaction detection chamber 117. Since the microfluidic analysis system adopts the above-mentioned microfluidic chip 100, the number of microfluidic chips 100 loaded can be customized according to the detection requirements, thereby avoiding the waste of microfluidic chips 100, samples, reagents, and diluents. In addition, during the detection process, the operator only needs to add samples and diluents, which is simple and convenient to operate.

[0147] As an embodiment, the turntable 200 is formed with a plurality of accommodating positions distributed sequentially along the circumference, each of which is used to accommodate a microfluidic chip 100 or a counterweight component having the same outer profile and the same weight as the microfluidic chip 100. The counterweight component is provided primarily to ensure the balance of the microfluidic chip 100 after being installed on the turntable 200. For example, if only one microfluidic chip 100 is required to meet the detection requirements, a counterweight component can be configured and arranged symmetrically with the microfluidic chip 100 on the turntable 200.

[0148] 9 , 10 and 11 , as an embodiment, the optical detection assembly 300 includes a light emitting element 310 and a light receiving element 320 . The light emitting element 310 is disposed above the turntable 200 to at least irradiate light toward the sample in the reaction detection chamber 117 . The light receiving element 320 is disposed below the turntable 200 and is located directly below the light emitting element 310 to receive light irradiated by the light emitting element 310 through the microfluidic chip 100 . The rotation drive mechanism 400 is used to drive the turntable 200 to rotate the microfluidic chip 100 to respectively achieve: quantification of the sample and the diluent, mixing of the sample and the diluent, distribution of the mixed liquid, and rotation of the reaction detection chamber 117 to directly below the light receiving element 320 . Specifically, the rotary drive mechanism 400 first drives the turntable 200 to drive the microfluidic chip 100 to perform the first centrifugal rotation to achieve quantification of the sample and the diluent; then drives the turntable 200 to drive the microfluidic chip 100 to perform the second centrifugal rotation to achieve mixing of the sample and the diluent; then drives the turntable 200 to drive the microfluidic chip 100 to perform the third centrifugal rotation to achieve distribution of the mixed liquid; finally, drives the turntable 200 to drive the microfluidic chip 100 to rotate so that each reaction detection chamber 117 rotates in sequence to directly below the light receiving element 320 for detection.

[0149] As an embodiment, the turntable 200 is formed with six accommodating positions distributed in sequence along the circumferential direction. Each microfluidic chip 100 has a structure with a central angle of 60°, and the counterweight component also has a structure with a central angle of 60°. The turntable 200 can carry six microfluidic chips 100 for testing at one time, or the turntable 200 can carry five microfluidic chips 100 and a counterweight component for testing at one time; or the turntable 200 can carry four symmetrically arranged microfluidic chips 100 for testing at one time; or the turntable 200 can carry three microfluidic chips 100 and a counterweight component for testing at one time; or the turntable 200 can carry two symmetrically arranged microfluidic chips 100 for testing at one time; or the turntable 200 can carry one microfluidic chip 100 and a counterweight component for testing at one time.

[0150] As an embodiment, when the turntable 200 carries two or more microfluidic chips 100 for detection at one time, the samples in the two microfluidic chips 100 can be samples from the same patient or samples from different patients.

[0151] As an embodiment, the microfluidic analysis system also includes a controller 500, which is electrically connected to the optical detection component 300 and the rotation drive mechanism 400, respectively. The controller 500 is used to control the rotation drive mechanism 400 to drive the turntable 200 to rotate and stop, and is used to analyze the detection data based on the feedback information of the optical detection component 300.

[0152] As an embodiment, the time duration that the rotary drive mechanism 400 drives the turntable 200 to rotate during the quantification stage of the sample and the diluent is greater than the time duration that the rotary drive mechanism 400 drives the turntable 200 to rotate during the mixing stage of the sample and the diluent, and is greater than the time duration that the rotary drive mechanism 400 drives the turntable 200 to rotate during the mixed liquid distribution stage.

[0153] As an implementation manner, the microfluidic chip 100 and the microfluidic analysis system provided in this embodiment can be applied to the detection of animal samples, and can also be applied to the detection of human samples.

[0154] As an implementation method, the workflow for sample detection using the microfluidic analysis system provided in this embodiment is as follows:

[0155] (1) Step 1: As shown in FIG2 , a certain amount (e.g., 20 ul to 50 ul) of sample (whole blood sample, plasma sample, or serum sample) is added into the injection chamber 111 from the sample injection hole 121 , and a certain amount (e.g., 50 ul to 100 ul) of diluent is added into the diluent chamber 113 from the diluent injection hole 122 .

[0156] (2) Step 2: Referring to FIG. 2 , FIG. 3 and FIG. 9 , the turntable 200 is driven to rotate at a first preset speed (e.g., a speed between 3000 rpm and 6000 rpm) for a first preset time (e.g., 3 min to 5 min) to allow the sample in the injection chamber 111 to flow into the first sample quantification chamber 112 and the second sample quantification chamber 105, and the excess sample to flow into the second overflow chamber 119. At the same time, the gas in the first sample quantification chamber 112, the second sample quantification chamber 105 and the second overflow chamber 119 is discharged from the second vent 124. In addition, the optical detection component 300 can be used to determine whether there is liquid in the sample determination chamber 101. If there is liquid, it can be determined that the sample volume is sufficient. Otherwise, it can be determined that the sample volume is insufficient. If the sample is a whole blood sample, in the later stage of the whole blood sample centrifugation process, the plasma will be concentrated in the first sample quantification chamber 112, and the red blood cells will be concentrated in the second sample quantification chamber 105.

[0157] As the sample is quantified, the diluent in the diluent chamber 113 flows into the diluent quantification chamber 114, and the excess diluent flows into the diluent determination chamber 102 and the first overflow chamber 118. Simultaneously, the gas originally in the diluent quantification chamber 114, the diluent determination chamber 102, and the first overflow chamber 118 is discharged through the first vent 123. The optical detection assembly 300 can be used to determine whether liquid is present in the diluent determination chamber 102. If liquid is present, it can be determined that the diluent is sufficient; otherwise, it can be determined that the diluent is insufficient.

[0158] In the latter part of the second step of the centrifugation process, plasma enters the sample drainage capillary 107. Due to the outward centrifugal force, the sample can only stay at position a1. Similarly, the diluent enters the diluent drainage capillary 108 and can only stay at position b1.

[0159] (3) Step 3: As shown in FIG3 , FIG4 and FIG9 , the turntable 200 stops rotating, the centrifugal force disappears, and the plasma in the sample drainage capillary 107 fills the sample drainage capillary 107 under the capillary force, and the plasma moves to position a2 of the sample drainage capillary 107. Similarly, the diluent fills the diluent drainage capillary 108, and the diluent moves to position b2 of the diluent drainage capillary 108.

[0160] (4) In the fourth step, as shown in Figures 4, 5 and 9, the turntable 200 rotates at a second preset speed (for example, a speed between 3000 rpm and 5000 rpm, the second preset speed can be less than or equal to or greater than the first preset speed) for a second preset time (for example, 10 seconds to 60 seconds, the second preset time is preferably less than the first preset time). The plasma in the first sample quantitative chamber 112 flows into the mixing chamber 115 through the sample drainage capillary 107 under the action of siphon. Similarly, the diluent in the diluent quantitative chamber 114 flows into the mixing chamber 115 through the diluent drainage capillary 108, and the gas in the mixing chamber 115 is discharged from the third vent 125. The turntable 200 mixes the diluent and plasma under the action of fast acceleration and slow deceleration. Due to the outward centrifugal force, the mixed liquid can only stay at the c1 position of the mixed liquid drainage capillary 109.

[0161] (5) Step 5: Referring to Figures 5, 6 and 9, the turntable 200 stops rotating, the centrifugal force disappears, the mixed liquid in the mixed liquid drainage capillary 109 fills the mixed liquid drainage capillary 109, and the mixed liquid moves to the c2 position of the mixed liquid drainage capillary 109.

[0162] (6) Step 6: Referring to Figures 6, 7, and 9, the turntable 200 is centrifuged at a third preset speed (e.g., 3000 rpm to 5000 rpm, which may be less than, equal to, or greater than the first preset speed) for a third preset time (e.g., 10 seconds to 60 seconds, which is preferably less than the first preset time). The mixed liquid in the mixing chamber 115 flows from the mixed liquid drainage capillary 109 into the distribution chamber 116. The initial liquid that enters the distribution chamber 116 from the mixed liquid drainage capillary 109 will preferentially enter the initial liquid chamber 1001, which is mainly used to load the unmixed liquid in the mixed liquid drainage capillary 109. The mixed liquid then flows into the reaction detection chamber 117, and the excess mixed liquid in the distribution chamber 116 flows into the second overflow chamber 119. At the same time, the gas originally in the distribution chamber 116 and the reaction detection chamber 117 is discharged from the second vent 124 through the diluent overflow channel 103. The freeze-dried ball reagent contained in the reaction detection chamber 117 can react with the mixed solution, and the detection of different items can be completed by optical signal collection.

[0163] The microfluidic chip 100 provided in this embodiment can make it possible for diluents and samples to be added without quantitative addition, and the microfluidic chip 100 can simultaneously meet the detection of different types of samples such as whole blood, plasma, and serum. During specific operations, the operator only needs to take samples within a certain range and diluents within a certain range and add them to the microfluidic chip 100 to complete the operation. The operation is very simple and convenient, and the amount of sample used is small and the quantification is accurate. Each microfluidic chip 100 constitutes a sub-disc that can be independently partitioned and independently detected. Multiple microfluidic chips 100 can be freely combined for detection, and the operator can customize the selection of the microfluidic chip 100 that needs to be re-inspected, which is conducive to reducing the cost of re-inspection and the waste of unnecessary consumables.

[0164] Example 2:

[0165] 1 , 8 and 12 , the microfluidic chip 100 and the microfluidic analysis system provided in this embodiment differ from those in the first embodiment mainly in the shape of the microfluidic chip 100 , specifically, the microfluidic chip 100 in the first embodiment is fan-shaped, while the microfluidic chip 100 in this embodiment is trapezoidal.

[0166] As an implementation, in this embodiment, the first edge 1005 and the second edge 1006 are two parallel linear edges, and the third edge 1007 and the fourth edge 1008 extend from the second edge 1006 to the first edge 1005 with a gradually decreasing spacing. In this embodiment, the microfluidic chip 100 has a trapezoidal structure.

[0167] Of course, in specific applications, the shape of the microfluidic chip 100 is not limited to fan-shaped and trapezoidal. For example, as an alternative embodiment, the microfluidic chip 100 can also be a rectangular structure. In this alternative embodiment, the first edge 1005 and the second edge 1006 are also two parallel straight edges, and the third edge 1007 and the fourth edge 1008 extend from the second edge 1006 to the first edge 1005 with a constant spacing.

[0168] Except for the above differences, other parts of the microfluidic chip 100 and the microfluidic analysis system provided in this embodiment can refer to the first embodiment and will not be described in detail here.

[0169] Example 3:

[0170] 1 , 8 and 13 , the microfluidic chip 100 and microfluidic analysis system provided in this embodiment differ from those in the first embodiment mainly in the different ways in which the overflow chamber is arranged. Specifically, in the first embodiment, the overflow of the diluent and the overflow of the mixed liquid share the overflow chamber, and the sample overflow chamber is independently arranged; whereas in this embodiment, the overflow of the sample and the overflow of the mixed liquid share the overflow chamber, and the overflow chamber of the diluent is independently arranged.

[0171] As an implementation method, in this embodiment, the non-circular chip body 110 is formed with a sample injection chamber 111, a first sample quantitative chamber 112, a diluent inlet chamber 113, a diluent quantitative chamber 114, a mixing chamber 115, a distribution chamber 116, a reaction detection chamber 117, a third overflow chamber 1011, and a fourth overflow chamber 1012; the third overflow chamber 1011 is connected to the diluent quantitative chamber 114 to collect the diluent overflowing from the diluent quantitative chamber 114; the fourth overflow chamber 1012 is connected to the first sample quantitative chamber 112 and the distribution chamber 116 respectively to collect the sample overflowing from the first sample quantitative chamber 112 and the mixed liquid overflowing from the distribution chamber 116. In this embodiment, the configuration and principle of the sample injection chamber 111, the first sample quantitative chamber 112, the diluent inlet chamber 113, the diluent quantitative chamber 114, the mixing chamber 115, the distribution chamber 116, and the reaction detection chamber 117 are the same as those in the first embodiment and will not be described in detail here. In this embodiment, since the fourth overflow chamber 1012 is used to collect the sample overflowing from the first sample quantification chamber 112 and to collect the mixed liquid overflowing from the distribution chamber 116, it is equivalent to combining the overflow chamber for sample quantification and the overflow chamber for the mixed liquid into one, which can also help reduce the number of overflow chambers, and further help simplify the structure of the microfluidic chip 100 and improve the structural compactness of the microfluidic chip 100, which can ultimately facilitate the miniaturization design of the microfluidic chip 100.

[0172] Except for the above differences, other parts of the microfluidic chip 100 and the microfluidic analysis system provided in this embodiment can refer to the first or second embodiment and will not be described in detail here.

[0173] The above description is only a preferred embodiment of the present application and does not limit the patent scope of the present application. All equivalent structural transformations made by using the contents of the present application description and drawings under the inventive concept of the present application, or direct / indirect application in other related technical fields are included in the patent protection scope of the present application.

Claims

1. A microfluidic chip, characterized in that: The chip comprises a non-circular chip body, wherein the non-circular chip body is formed with a sample injection cavity, a first sample quantitative cavity, a diluent inlet cavity, a diluent quantitative cavity, a mixing cavity, a distribution cavity, a reaction detection cavity, a first overflow cavity, and a second overflow cavity; The injection cavity is used to store samples entering the microfluidic chip; The first sample quantification chamber is in communication with the injection chamber, so as to quantify the sample from the injection chamber when the microfluidic chip is centrifuged; The diluent inlet cavity is used to store the diluent entering the microfluidic chip; The diluent quantitative chamber is in communication with the diluent inlet chamber, so as to quantitatively measure the diluent from the diluent inlet chamber when the microfluidic chip is centrifuged; The mixing chamber is communicated with the first sample quantitative chamber and the diluent quantitative chamber respectively, so as to receive and mix the sample entering from the first sample quantitative chamber and the diluent entering from the diluent quantitative chamber when the microfluidic chip is centrifuged; The distribution chamber is communicated with the mixing chamber and the reaction detection chamber respectively, so as to receive a mixed solution formed by mixing the sample and the diluent from the mixing chamber when the microfluidic chip is centrifuged, and distribute the mixed solution to the reaction detection chamber; The reaction detection chamber is used for the reagent to react with the mixed solution to form a sample; The first overflow chamber is communicated with the diluent quantitative chamber and the distribution chamber respectively, so as to collect the diluent overflowing from the diluent quantitative chamber and the mixed liquid overflowing from the distribution chamber; The second overflow chamber is communicated with the first sample quantification chamber to collect the sample overflowing from the first sample quantification chamber.

2. The microfluidic chip according to claim 1, wherein: The non-circular chip body is further formed with a sample determination cavity, which is in communication with the second overflow cavity, so as to allow the optical detection component to detect whether there is a sample overflowing from the first sample quantification cavity; The distance between the sample determination chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip is the same as the distance between the reaction detection chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip.

3. The microfluidic chip according to claim 2, wherein: The non-circular chip body is further formed with a diluent determination chamber, which is in communication with the first overflow chamber, so as to allow an optical detection component to detect whether there is diluent overflowing from the diluent quantitative chamber; The distance between the diluent determination chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip is the same as the distance between the reaction detection chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip.

4. The microfluidic chip according to claim 3, wherein: The distance between the diluent judgment chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip is greater than the distance between the first overflow chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip.

5. The microfluidic chip according to claim 3 or 4, wherein: The sample judgment chamber and the diluent judgment chamber are respectively located on both sides of the reaction detection chamber pair along the direction of centrifugal rotation of the microfluidic chip.

6. The microfluidic chip according to any one of claims 1 to 4, wherein: The non-circular chip body also forms a first channel and a second channel, the two ends of the first channel are respectively connected to the distribution chamber and the first overflow chamber, the two ends of the second channel are respectively connected to the distribution chamber and the reaction detection chamber, and the width of the first channel in the centrifugal rotation direction of the microfluidic chip is equal to the width of the second channel in the centrifugal rotation direction of the microfluidic chip.

7. The microfluidic chip according to any one of claims 1 to 4, wherein: The non-circular chip body has a first plate surface and a second plate surface disposed opposite to each other, and the sample injection chamber, the first sample quantitative chamber, the diluent inlet chamber, the diluent quantitative chamber, the mixing chamber, the distribution chamber, the reaction detection chamber, the first overflow chamber, and the second overflow chamber are all recessed from the first plate surface toward the second plate surface, and are spaced apart from the second plate surface. The microfluidic chip further includes a sealing film, which is attached to the first plate surface to cover at least the sample injection chamber, the first sample quantitative chamber, the diluent inlet chamber, the diluent quantitative chamber, the mixing chamber, the distribution chamber, the reaction detection chamber, the first overflow chamber, and the second overflow chamber; The sealing film is provided with a sample injection hole at a position corresponding to the injection cavity. The sample injection hole is communicated with the injection cavity for injecting the sample into the injection cavity.

8. The microfluidic chip according to claim 7, wherein: The sealing film is provided with a diluent injection hole at a portion corresponding to the diluent inlet cavity, and the diluent injection hole is communicated with the diluent inlet cavity for injecting the diluent into the diluent inlet cavity; or A diluent bag is placed in the diluent inlet cavity.

9. The microfluidic chip according to claim 7, wherein: The non-circular chip body is further formed with a diluent overflow channel and a sample overflow channel, wherein the diluent overflow channel is connected between the diluent quantitative chamber and the first overflow chamber, and the sample overflow channel is connected between the first sample quantitative chamber and the second overflow chamber; The sealing film is further penetrated by a first vent hole, a second vent hole and a third vent hole. The first vent hole is communicated with the diluent overflow channel, the second vent hole is communicated with the sample overflow channel, and the third vent hole is communicated with the mixing chamber.

10. The microfluidic chip according to any one of claims 1 to 4, characterized in that: The non-circular chip body is further formed with a second sample quantification cavity, a sample quantification channel and a sample drainage capillary, and the two ends of the sample quantification channel are respectively connected to the first sample quantification cavity and the second sample quantification cavity; The distance between the sample quantification channel and the central axis of rotation of the centrifugal rotation of the microfluidic chip is greater than the distance between the first sample quantification chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip, and is less than the distance between the second sample quantification chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip; The two ends of the sample drainage capillary are respectively connected to the sample quantitative channel and the mixing chamber, and the sample drainage capillary has a first bending portion, and the distance between the first bending portion and the central axis of rotation of the centrifugal rotation of the microfluidic chip is smaller than the distance between the first sample quantitative chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip.

11. The microfluidic chip according to any one of claims 1 to 4, characterized in that: The non-circular chip body is further formed with a mixed liquid drainage capillary and an initial liquid cavity; The two ends of the mixed liquid drainage capillary are respectively connected to the mixing chamber and the distribution chamber, and the mixed liquid drainage capillary has a second bent portion, and the distance between the second bent portion and the central axis of rotation of the centrifugal rotation of the microfluidic chip is smaller than the distance between the mixing chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip; The initial liquid chamber is in communication with one end of the distribution chamber close to the mixed liquid drainage capillary, so as to at least collect the initial liquid entering the distribution chamber from the mixed liquid drainage capillary; The distance between the initial liquid chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip is smaller than the distance between the reaction detection chamber and the central axis of rotation of the centrifugal rotation of the microfluidic chip.

12. The microfluidic chip according to claim 11, wherein: The volume of the initial liquid chamber is smaller than the volume of the reaction detection chamber; and / or, The non-circular chip body also forms a second channel and a third channel, the two ends of the second channel are respectively connected to the distribution chamber and the reaction detection chamber, the two ends of the third channel are respectively connected to the distribution chamber and the initial liquid chamber, and the width of the third channel in the centrifugal rotation direction of the microfluidic chip is equal to the width of the second channel in the centrifugal rotation direction of the microfluidic chip.

13. The microfluidic chip according to claim 1, wherein: The non-circular chip body includes a first edge and a second edge, the first edge and the second edge are spaced apart and arranged opposite to each other, the first edge is provided at an end of the microfluidic chip close to the central axis of rotation of the microfluidic chip centrifugally, and the second edge is provided at an end of the microfluidic chip away from the central axis of rotation of the microfluidic chip centrifugally; The diluent inlet chamber, the diluent quantitative chamber, the mixing chamber, the distribution chamber, and the first overflow chamber are sequentially arranged between the first edge and the second edge; The injection chamber, the first sample quantitative chamber, the mixing chamber, the distribution chamber, and the second overflow chamber are sequentially arranged between the first edge and the second edge.

14. The microfluidic chip according to claim 13, wherein: The length of the second edge is greater than the length of the first edge.

15. The microfluidic chip according to claim 13, wherein: The first edge and the second edge are two arc-shaped edges with the same center; or, The first edge and the second edge are two straight edges parallel to each other.

16. The microfluidic chip according to any one of claims 13 to 15, wherein: The non-circular chip body further includes a third edge and a fourth edge; The third edge is spaced apart from the fourth edge and is arranged opposite to each other. The third edge extends from one end of the first edge to one end of the second edge, and the fourth edge extends from the other end of the first edge to the other end of the second edge. The diluent inlet chamber, the diluent quantitative chamber, one end of the mixing chamber, one end of the distribution chamber, and the first overflow chamber are arranged in sequence along the third edge; The injection chamber, the first sample quantitative chamber, and the second overflow chamber are arranged in sequence along the fourth edge.

17. The microfluidic chip according to claim 16, wherein: An angle formed by the third edge and the fourth edge is greater than 0° and less than or equal to 90°.

18. The microfluidic chip according to claim 17, wherein: The included angle formed by the third edge and the fourth edge is 60°±15°.

19. The microfluidic chip according to any one of claims 1 to 4 or any one of claims 13 to 15, wherein: The number of the reaction detection cavities formed by the non-circular chip body is less than or equal to six.

20. The microfluidic chip according to claim 19, wherein: The number of the reaction detection cavities formed by the non-circular chip body is two, three, four or five.

21. A microfluidic chip, characterized in that: The non-circular chip body is formed with a sample injection cavity, a first sample quantitative cavity, a diluent inlet cavity, a diluent quantitative cavity, a mixing cavity, a distribution cavity, a reaction detection cavity, a third overflow cavity, and a fourth overflow cavity; The first sample quantification chamber is in communication with the injection chamber, so as to quantify the sample from the injection chamber when the microfluidic chip is centrifuged; The diluent quantitative chamber is in communication with the diluent inlet chamber, so as to quantitatively measure the diluent from the diluent inlet chamber when the microfluidic chip is centrifuged; The mixing chamber is communicated with the first sample quantitative chamber and the diluent quantitative chamber respectively, so as to receive and mix the sample entering from the first sample quantitative chamber and the diluent entering from the diluent quantitative chamber when the microfluidic chip is centrifuged; The distribution chamber is communicated with the mixing chamber and the reaction detection chamber respectively, so as to receive a mixed solution formed by mixing the sample and the diluent from the mixing chamber when the microfluidic chip is centrifuged, and distribute the mixed solution to the reaction detection chamber; The reaction detection chamber is used for the reagent to react with the mixed solution to form a sample; The third overflow chamber is in communication with the diluent quantitative chamber, and is used to collect the diluent overflowing from the diluent quantitative chamber; The fourth overflow chamber is communicated with the first sample quantitative chamber and the distribution chamber respectively, so as to collect the sample overflowing from the first sample quantitative chamber and the mixed liquid overflowing from the distribution chamber.

22. A microfluidic analysis system, characterized in that: comprising a turntable, an optical detection component, a rotation drive mechanism, and the microfluidic chip according to any one of claims 1 to 21; The turntable is formed with a plurality of accommodating positions distributed in sequence along the circumferential direction, each of the accommodating positions is used to accommodate one of the microfluidic chips or a counterweight component with the same outer contour and the same weight as the microfluidic chip; The optical detection assembly includes a light emitting element and a light receiving element, wherein the light emitting element is disposed above the turntable and is used to at least irradiate light toward the sample in the reaction detection chamber; The light receiving element is provided below the turntable and directly below the light emitting element, so as to receive the light emitted by the light emitting element through the microfluidic chip; The rotary drive mechanism is used to drive the turntable to drive the microfluidic chip to rotate, so as to respectively achieve: quantification of the sample and the diluent, mixing of the sample and the diluent, distribution of the mixed solution, and rotation of the reaction detection chamber to directly below the light receiving element.

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