Litopenaeus vannamei factory circulating water marking system and method

Abstract

The invention relates to a factory circulating water calibration system and method for penaeus vannamei boone. The calibration method comprises the following steps: step 1, constructing a circulating water treatment system comprising a juvenile penaeus vannamei culture pond, a settling pond, a microfilter, a biochemical pond, an oxygenation device, a temperature control device and a water pump; 2, a staged water treatment process is adopted, a biofloc technology for directional culture of a nano-cluster flora carrier is used in the early stage, and a biofilm nitrification technology is combined in the later stage; step 3, forming a bacterium-algae composite biological floc through a nano-cluster carrier material, wherein the carrier material is composed of a nano-scale porous silicate composite microorganism immobilization carrier; 4, a dynamic feeding model is established, an intelligent feeding system is adopted to implement a gradient feeding strategy, and the feeding amount and glucose are matched according to the ratio of 1: 2; and step 5, implementing a progressive salinity adjustment process, completing salinity transition from 20% o to 7% o at the rate of reducing 2.6% o every day in 6-10 days, and adjusting the rate to be less than or equal to 0.5% o per hour.

Description

Technical Field

[0001] The invention relates to the technical field of aquaculture, and in particular to a system and method for industrialized circulating water standardization of white shrimp. Background Art

[0002] The standardization of industrial circulating water for white shrimp refers to the refined breeding stage of shrimp seedlings in a high-density, controllable circulating water aquaculture system. Its core goal is to improve the survival rate, adaptability and uniformity of shrimp seedlings through standardized management, laying the foundation for subsequent shrimp farming.

[0003] Among the existing technologies, the traditional shrimp farming density is low (less than 10,000 shrimp / m 3 ), water quality control difficulties, frequent diseases and other problems. The existing biofloc technology has poor stability and is easily affected by temperature fluctuations (>±2℃) and salinity changes (>5% / day), which leads to an imbalance in the bacterial flora. The removal rate of ammonia nitrogen and nitrite nitrogen in the circulating water system is low, which greatly affects the survival rate of shrimp. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention aims to provide a system and method for industrial circulating water standardization of white shrimp, which forms stable bioflocs through the directional cultivation technology of nano-cluster bacteria, combines the phased water treatment process and the intelligent feeding model, and achieves a standard density of 50,000 shrimp / m 3 The above, the breeding cycle is 30 days, and the survival rate is >90%; the system includes modular breeding units, three-level water treatment modules and intelligent environmental control devices, with the advantages of stable water quality, disease prevention and control, and high feed efficiency.

[0005] The above-mentioned object of the present invention is achieved through the following technical solutions:

[0006] A method for industrial circulating water standardization of Penaeus vannamei comprises the following steps:

[0007] Step 1, constructing a circulating water treatment system including a shrimp breeding pond, a sedimentation tank, a microfiltration machine, a biochemical pool, an oxygenation device, a temperature control device, and a water pump;

[0008] Step 2: adopt a phased water treatment process, using the biofloc technology of nano-cluster bacterial carrier directional culture in the early stage, and combining biofilm nitrification technology in the later stage;

[0009] Step 3, forming bacterial-algal composite bioflocs by using a nanocluster carrier material, wherein the carrier material is composed of a nanoscale porous silicate composite microorganism immobilization carrier;

[0010] Step 4: Establish a dynamic feeding model, use an intelligent feeding system to implement a gradient feeding strategy, and mix the feeding amount with glucose in a ratio of 1:2;

[0011] Step 5: Implement a progressive salinity adjustment process to complete the salinity transition from 20‰ to 7‰ at a rate of 2.6‰ per day in 6-10 days, with an adjustment rate of ≤0.5‰ per hour.

[0012] As a further technical solution of the present invention: the biofloc technology comprises the following steps:

[0013] S1. Adding nano-cluster directional bacterial complexes, including nitrifying bacteria, Bacillus and photosynthetic bacteria, into aquaculture water;

[0014] S2, maintain dissolved oxygen ≥ 5 mg / L, temperature 29-31 ° C, pH 7.5-8.5;

[0015] S3, forming stable flocs with a particle size of 50-200μm, and the floc density is maintained at 3-5g / L.

[0016] As a further technical solution of the present invention: the bacterial population ratio of the nitrifying bacteria, the Bacillus and the photosynthetic bacteria is 5:3:2.

[0017] As a further technical solution of the present invention: in step 2, the nanocluster bacteria carrier is composited by porous diatomaceous earth and sodium alginate in a mass ratio of 3:1.

[0018] As a further technical solution of the present invention: the pore size of the porous diatomaceous earth is 20-50 nm.

[0019] As a further technical solution of the present invention: in the step 2, the phased water treatment process includes the following steps: using the biofloc technology from 10 days before stocking to the 5th day of breeding, the biofloc is formed by loading a composite bacterial community on a nanocluster carrier; starting from the 6th day of breeding, the MBBR biofilm nitrification mode is started using the biofilm nitrification technology.

[0020] As a further technical solution of the present invention: the particle size of the biofloc is 80-150 μm, the density is 3.5-4.2 g / L, the carbon-nitrogen ratio (C / N) is maintained at 15-20:1, and the floc structure is maintained by an ultrasonic stabilization device (frequency 28 kHz±5%, power density 0.5 W / L).

[0021] As a further technical solution of the present invention: in step 4, the intelligent feeding system includes:

[0022] Leftover bait identification module: Identify leftover bait particles based on the YOLOv5 algorithm, with a detection accuracy of ≥90%;

[0023] Feedback control module: Fuzzy PID controller is used to dynamically adjust the feeding amount according to the amount of residual bait. The adjustment formula is: Q n+1 =Qn ×[1-0.2×(R n / S n )], where Q n is the feeding amount for the nth time, R n is the amount of residual bait, S n is the standard food intake;

[0024] Feeding actuator: pneumatic conveying feeder, feeding error ≤±5%.

[0025] A circulating water roughing system of the above-mentioned industrial circulating water roughing method for white shrimp comprises:

[0026] Breeding unit: circular pool with a diameter of 5-7m, nano-coating on the pool wall (contact angle>150°), bottom nano-aeration pipe network (aeration density 0.3-0.5m 3 / h·m 2 );

[0027] Water treatment unit: cyclone sedimentation tank (hydraulic retention time HRT = 30min), rotary drum microfilter (120 mesh screen), biofloc reactor (HRT = 3h, dissolved oxygen DO = 6-8mg / L), MBBR reactor (filling rate 40%, K3 filler) connected in sequence;

[0028] Environmental control unit: includes PID temperature control module (accuracy ±0.5℃), dissolved oxygen feedback system (PID adjusts aeration volume), and pH automatic compensation device (sodium bicarbonate addition rate 0-5g / min).

[0029] As a further technical solution of the present invention: the nano aeration pipe network is made of a hydrophobic polytetrafluoroethylene membrane with a pore size distribution of 30-50nm and an oxygen utilization rate of ≥85%.

[0030] In summary, the present invention includes at least one of the following beneficial technical effects:

[0031] 1. The present invention discloses a system and method for standardizing the circulating water of Penaeus vannamei in a factory, which forms a stable biofloc by fixing the composite bacterial community through a nanocluster carrier, combines a graded water treatment process (biofloc → MBBR biofilm) and an intelligent feeding system, and achieves a standardizing density of ≥50,000 shrimp / m 3 , ammonia nitrogen removal rate> 90%, feed coefficient <1.2. The system includes nano-aeration culture pond, three-stage water treatment module and feeding control system based on machine vision, which is suitable for multi-crop factory culture of white shrimp throughout the year.

[0032] 2. The roughing stage of the shrimp of the present invention is not affected by external conditions and can be carried out 10 times throughout the year, which improves the production efficiency throughout the year and provides a basis for multi-crop farming of shrimp in one year. The specification of roughing juvenile shrimp reaches about 500 tails / jin, which increases the nutritional basis of shrimp fry in the early stage, improves the growth rate of each stage of shrimp farming, shortens the adult shrimp farming cycle, and facilitates multi-season shrimp farming. The biofloc and biofilm nitrification water treatment technology is used in stages to solve the problem of harmful substances such as ammonia nitrogen and nitrite nitrogen generated during the breeding process, improves the breeding density in the roughing stage, and increases the roughing production capacity and efficiency. The biofloc method of circulating water white shrimp breeding technology uses nano-cluster bacterial community directional materials and forms stable water biological flocs through specific bacterial community cultivation methods.

[0033] 2. The present invention has good stability. Compared with the traditional flocculant technology, the biofloc formed by the nanocluster carrier has the greatest feature of good stability. Once the nanocluster flocculants are formed, they have long-term stability and are relatively tolerant to temperature, salinity, common fungicides and environmental changes. They are effective in inhibiting bacteria and viruses. Once the nanocluster bioflocs are formed, they can effectively inhibit the reproduction of environmental bacteria and viruses and directionally amplify the probiotic system.

[0034] 3. The present invention can effectively control water quality indicators, especially in high-density breeding environments, where a large number of shrimp biological metabolism produces a lot of metabolic toxins (mainly ammonia nitrogen and nitrite). As long as the biological flocs are effectively formed, ammonia nitrogen and nitrite can be decomposed and converted in situ, and ammonia nitrogen and nitrite indicators can be controlled within the safety precautions for shrimp production.

[0035] 4. The nano-cluster flocs of the present invention are a bacterial-algal composite system, which further improves the utilization rate of shrimp to feed through the algae's absorption and conversion of shrimp feces and metabolites, effectively reducing the feed ratio coefficient in shrimp farming. A shrimp standard coarse stage feeding model is established, and intelligent feeding equipment is used to effectively reduce labor costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is a structural schematic diagram of the circulating water treatment system in the present invention.

[0037] Figure 2 This is a schematic diagram of the main pathogen detection report of shrimp in the present invention. DETAILED DESCRIPTION

[0038] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application; obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments, and all other embodiments obtained by ordinary technicians in this field based on the embodiments in the present application without making creative work are within the scope of protection of the present application.

[0039] In the description of the present application, it should be noted that the terms "upper", "lower", "inner", "outer", "top / bottom" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as limiting the present application. In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0040] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "set / connected", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0041] Embodiment 1:

[0042] The invention discloses a method for industrialized circulating water standardization of white shrimp, comprising the following steps:

[0043] Step 1: Construct a circulating water treatment system including a shrimp breeding pond, a sedimentation tank, a microfiltration machine, a biochemical pool, an oxygenation device, a temperature control device, and a water pump (see Figure 1 );

[0044] Step 2: adopt a phased water treatment process, using the biofloc technology of nano-cluster bacterial carrier directional culture in the early stage, and combining biofilm nitrification technology in the later stage;

[0045] Step 3, forming bacterial-algal composite bioflocs by using nano-cluster carrier materials, wherein the carrier materials are composed of nano-scale porous silicate composite microorganism immobilization carriers;

[0046] Step 4: Establish a dynamic feeding model, use an intelligent feeding system to implement a gradient feeding strategy, and mix the feeding amount with glucose in a ratio of 1:2;

[0047] Step 5: Implement a progressive salinity adjustment process to complete the salinity transition from 20‰ to 7‰ at a rate of 2.6‰ per day in 6-10 days, with an adjustment rate of ≤0.5‰ per hour.

[0048] Biofloc technology includes the following steps:

[0049] S1. Adding nano-cluster directional bacterial complexes, including nitrifying bacteria, Bacillus and photosynthetic bacteria, into aquaculture water;

[0050] S2, maintain dissolved oxygen ≥ 5 mg / L, temperature 29-31 ° C, pH 7.5-8.5;

[0051] S3, forming stable flocs with a particle size of 50-200μm, and the floc density is maintained at 3-5g / L.

[0052] Among them, the bacterial community ratio of nitrifying bacteria, Bacillus and photosynthetic bacteria is 5:3:2. In step 2, the nanocluster bacterial community carrier is composited by porous diatomaceous earth and sodium alginate in a mass ratio of 3:1, and the pore size of the porous diatomaceous earth is 20-50nm. In step 2, the phased water treatment process includes the following steps: using the biofloc technology from 10 days before seedlings to the 5th day of breeding, and the biofloc is formed by loading the composite bacterial community with the nanocluster carrier; starting from the 6th day of breeding, the MBBR biofilm nitrification mode is started using the biofilm nitrification technology.

[0053] The particle size of the biofloc is 80-150 μm, the density is 3.5-4.2 g / L, the carbon-nitrogen ratio (C / N) is maintained at 15-20:1, and the floc structure is maintained by an ultrasonic stabilization device (frequency 28 kHz±5%, power density 0.5 W / L).

[0054] In step 4, the intelligent feeding system includes:

[0055] Leftover bait identification module: Identify leftover bait particles based on the YOLOv5 algorithm, with a detection accuracy of ≥90%;

[0056] Feedback control module: Fuzzy PID controller is used to dynamically adjust the feeding amount according to the amount of residual bait. The adjustment formula is: Q n+1 =Q n ×[1-0.2×(R n / S n )], where Q n is the feeding amount for the nth time, R n is the amount of residual bait, S n is the standard food intake;

[0057] Feeding actuator: pneumatic conveying feeder, feeding error ≤±5%.

[0058] In this embodiment, the roughing cycle is from P5 juvenile shrimp to juvenile shrimp of specification 1 g / tail, and this stage is about 30 days.

[0059] The juvenile shrimp farming process specifically includes the following steps:

[0060] (1) According to the base plan, formulate a procurement plan for materials and shrimp seedlings, and all materials required for the juvenile shrimp breeding stage should be in place one day before the shrimp seedlings arrive;

[0061] (2) Cleaning and tidying up the shrimp pond:

[0062] A. Check and maintain the heating system, aeration system, and circulation system to ensure that the equipment can work normally;

[0063] B. Use potassium permanganate with a concentration of 4% to disinfect the aquaculture system, mainly to kill bacteria, parasites, etc.;

[0064] (3) Prepare the aquaculture water 10 days in advance, aerate, remove dissolved organic matter, and cultivate algae. The prepared aquaculture water is crisp green when observed with the naked eye;

[0065] (4) Salinity of water used for preparing juvenile shrimp (P5): above 20‰;

[0066] (5) One day before stocking, retest the salinity, temperature, pH, dissolved oxygen, ammonia nitrogen, and nitrite concentrations of the water used for shrimp farming to ensure that everything is safe; in spring, autumn, and winter, the water temperature should be controlled between 22 and 24°C; the pH should be controlled between 7.5 and 8.5; the dissolved oxygen concentration should be above 7 mg / L; and the ammonia nitrogen and nitrite concentrations should be below 0.1 mg / L. After the test is completed, the water should be registered. If any item fails to meet the standard, stocking will be postponed until it meets the standard;

[0067] (6) When releasing the shrimp, first check the activity of the shrimp fry and observe whether there are any white fry. If the activity of the shrimp fry is not good and there are any white fry, discard them;

[0068] (7) Take four samples from the same batch of shrimp seedlings for virus testing, one for third-party testing, one for internal testing, and two for backup;

[0069] (8)Reference Figure 2 , which is the sample test results of the main shrimp pathogen detection report;

[0070] (9) After the shrimp fry arrive, test the pH of the water in the fry bag. The pH of artificially prepared seawater is generally between 8.2-8.5; the pH of the water for the fry is lower, between 7.2-7.5; therefore, after the shrimp fry arrive, they need to be washed; pour the shrimp fry into a temporary holding bucket (whose volume is more than 3 times the water body to be released), fully aerate, and ensure that the dissolved oxygen concentration is above 7mg / L. Every 2-3 minutes, add 30% of the water in the fry bag into the temporary holding bucket. The entire frying process lasts about 2 hours; when the pH and temperature are consistent inside and outside, release the fry into the standard coarse bucket, with a stocking density of 50,000 tails / m 3 .

[0071] (10) Within 12 hours after stocking, the water temperature should be slowly raised to 27°C, and within 12-24 hours, the water temperature should be slowly raised to 31°C. The shrimp juvenile management personnel should regularly check the water temperature;

[0072] (11) On the day of stocking, start adding shrimp powder feed; for the first time, if the diameter of the pond is 5 meters, add 150g; if the diameter of the pond is 6 meters, add 200g; if the diameter of the pond is 7 meters, add 250g;

[0073] (12) Feeding shrimp powder: Establish a feeding parameter model for the standard and rough stage of shrimp, use intelligent feeding technology, feed six times a day, from 8 am to 8 pm, and feed once every 2 hours on average. Mix glucose at the same time, and the amount is twice the amount of feed fed at that time. Check the remaining bait regularly, and decide the next feeding amount based on the remaining bait situation, and repeat this process;

[0074] (13) Test water quality indicators at 8:00, 14:00, and 20:00 every day to ensure that the water quality indicators are normal; especially the indicators of ammonia nitrogen and nitrite, and judge whether the biofloc is functioning normally based on their concentration;

[0075] (14) During the shrimp farming period, the water temperature is maintained between 29 and 31°C; the pH is controlled between 7.5 and 8.5; the dissolved oxygen concentration is above 5.0 mg / L; the ammonia nitrogen and nitrous oxide are below 1.5 mg / L and 0.5 mg / L respectively. If any of the water quality indicators cannot be met, appropriate measures should be taken to make timely adjustments;

[0076] (15) On the sixth day of culture, water was slowly added to desalinate the water. On the tenth day, the water content was reduced to about 7‰, and the desalination cycle was completed.

[0077] (16) Weigh the average weight of shrimp regularly every week and evaluate the shrimp pond stock based on the average weight and number of shrimp;

[0078] (17) When the juvenile shrimp farming is finished, the shrimp are sampled and weighed. Then, the water is drained and the pond is lowered. The juvenile shrimp are caught with a scoop net, weighed, and transported to the adult shrimp pond. When catching, the pond bottom is fully aerated and oxygenated. The amount caught each time should not be too large to prevent death due to lack of oxygen.

[0079] The following is a table of water quality parameters for whiteleg shrimp:

[0080]

[0081]

[0082] Embodiment 2:

[0083] A circulating water roughing system of the above-mentioned industrial circulating water roughing method for white shrimp comprises:

[0084] Breeding unit: circular pool with a diameter of 5-7m, nano-coating on the pool wall (contact angle>150°), bottom nano-aeration pipe network (aeration density 0.3-0.5m 3 / h·m2 );

[0085] Water treatment unit: cyclone sedimentation tank (hydraulic retention time HRT = 30min), rotary drum microfilter (120 mesh screen), biofloc reactor (HRT = 3h, dissolved oxygen DO = 6-8mg / L), MBBR reactor (filling rate 40%, K3 filler) connected in sequence;

[0086] Environmental control unit: includes PID temperature control module (accuracy ±0.5℃), dissolved oxygen feedback system (PID adjusts aeration volume), and pH automatic compensation device (sodium bicarbonate addition rate 0-5g / min).

[0087] In this embodiment, the nano-aeration pipe network is made of a hydrophobic polytetrafluoroethylene membrane with a pore size distribution of 30-50 nm and an oxygen utilization rate of ≥85%.

[0088] The core technology of this invention is: Nanocluster biological carrier technology: the carrier specific surface area is 800-1000m 2 / g, the bacterial load is 3 times higher than that of traditional carriers; the diffusion of bacteria is limited by diatomaceous earth nanopores to maintain the stability of the bacterial ratio (coefficient of variation <5%).

[0089] Gradual water treatment process: Early stage (1-5 days): biological floc mode quickly degrades ammonia nitrogen (efficiency> 90%); late stage (6-30 days): MBBR biofilm mode stably controls nitrite (<0.2mg / L).

[0090] Intelligent feeding system: The accuracy rate of residual bait identification is 92.3%, and the feed coefficient is reduced to below 1.2; combined with glucose feeding, floc formation is promoted, and the carbon source utilization rate is increased by 40%.

[0091] Example 3: Shrimp crude production test:

[0092] Breeding pond: diameter 6m, water depth 1m, stocking density 52,000 fish / m 3 ; Water quality control: temperature 30±0.5℃ (PID control); dissolved oxygen 6.5±0.3mg / L; pH 8.0±0.2 (automatic compensation system). Results: 30-day average weight 1.08g, survival rate 93.5%; ammonia nitrogen peak 0.35mg / L, nitrite peak 0.18mg / L; feed coefficient 1.15.

[0093] Example 4: Nanocarrier preparation process:

[0094] Diatomaceous earth nanoparticles (D50 = 80nm) and sodium alginate solution (3wt%) were mixed at a ratio of 3:1; injected into a mold and freeze-dried (-40°C, 24h) to form a porous block; impregnated with a composite bacterial solution (bacterial concentration 10 8CFU / mL) and then vacuum packaged.

[0095] Example 5: Verification of residual bait identification algorithm:

[0096] Dataset: 5,000 images of bait residue were collected (including scenes with different lighting and turbidity); Model training: YOLOv5 network, 300 epochs; Test results: precision rate 92.7%, recall rate 91.4%.

[0097] The implementation principle of the present invention is as follows: the present invention discloses a system and method for standardizing the circulating water of Penaeus vannamei in a factory, which forms a stable biofloc by fixing the composite bacterial community through a nanocluster carrier, combines a graded water treatment process (biofloc → MBBR biofilm) and an intelligent feeding system, and achieves a standardizing density of ≥50,000 shrimp / m 3 , ammonia nitrogen removal rate> 90%, feed coefficient <1.2. The system includes nano-aeration culture pond, three-stage water treatment module and feeding control system based on machine vision, which is suitable for multi-crop factory culture of white shrimp throughout the year.

[0098] The embodiments of this specific implementation method are all preferred embodiments of the present invention, and are not intended to limit the protection scope of the present invention. Therefore, all equivalent changes made based on the structure, shape, and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for industrial circulating water standardization of Penaeus vannamei, characterized in that: The following steps are involved: Step 1, constructing a circulating water treatment system including a shrimp breeding pond, a sedimentation tank, a microfiltration machine, a biochemical pool, an oxygenation device, a temperature control device, and a water pump; Step 2: adopt a phased water treatment process, using the biofloc technology of nano-cluster bacterial carrier directional culture in the early stage, and combining biofilm nitrification technology in the later stage; Step 3, forming bacterial-algal composite bioflocs by using a nanocluster carrier material, wherein the carrier material is composed of a nanoscale porous silicate composite microorganism immobilization carrier; Step 4: Establish a dynamic feeding model, use an intelligent feeding system to implement a gradient feeding strategy, and mix the feeding amount with glucose in a ratio of 1:2; Step 5: Implement a progressive salinity adjustment process to complete the salinity transition from 20‰ to 7‰ at a rate of 2.6‰ per day in 6-10 days, with an adjustment rate of ≤0.5‰ per hour.

2. A method for industrialized circulating water treatment of Penaeus vannamei according to claim 1, characterized in that: The biofloc technology comprises the following steps: S1. Adding nano-cluster directional bacterial complexes, including nitrifying bacteria, Bacillus and photosynthetic bacteria, into aquaculture water; S2, maintain dissolved oxygen ≥ 5 mg / L, temperature 29-31 ° C, pH 7.5-8.5; S3, forming stable flocs with a particle size of 50-200μm, and the floc density is maintained at 3-5g / L.

3. A system and method for industrialized circulating water standardization of white shrimp according to claim 2, characterized in that: The bacterial population ratio of the nitrifying bacteria, the Bacillus and the photosynthetic bacteria is 5:3:

2.

4. A method for industrialized circulating water standardization of Penaeus vannamei according to claim 1, characterized in that: In the step 2, the nanocluster bacterial community carrier is composited by porous diatomaceous earth and sodium alginate in a mass ratio of 3:

1.

5. A method for industrialized circulating water standardization of Penaeus vannamei according to claim 4, characterized in that: The pore size of the porous diatomaceous earth is 20-50 nm.

6. A method for industrialized circulating water standardization of Penaeus vannamei according to claim 1, characterized in that: In step 2, the phased water treatment process The method comprises the following steps: using the biofloc technology from 10 days before stocking to the 5th day of breeding, wherein the biofloc is formed by loading a composite bacterial community on a nanocluster carrier; and starting the MBBR biofilm nitrification mode from the 6th day of breeding by using the biofilm nitrification technology.

7. A system and method for industrialized circulating water treatment of Penaeus vannamei according to claim 6, characterized in that: The particle size of the biological flocs is 80-150 μm, the density is 3.5-4.2 g / L, the carbon-nitrogen ratio is maintained at 15-20:1, and the floc structure is maintained by an ultrasonic stabilization device.

8. A system and method for industrialized circulating water standardization of Penaeus vannamei according to claim 1, characterized in that: In step 4, the intelligent feeding system includes: Leftover bait identification module: Identify leftover bait particles based on the YOLOv5 algorithm, with a detection accuracy of ≥90%; Feedback control module: Fuzzy PID controller is used to dynamically adjust the feeding amount according to the amount of residual bait. The adjustment formula is: Q n+1 =Q n ×[1-0.2×(R n / S n )], where Q n is the feeding amount for the nth time, R n is the amount of residual bait, S n is the standard food intake; Feeding actuator: pneumatic conveying feeder, feeding error ≤±5%.

9. A circulating water roughing system for realizing the industrialized circulating water roughing method for Penaeus vannamei as described in any one of claims 1 to 8, characterized in that: include: Breeding unit: a circular pool with a diameter of 5-7m, a nano-coating on the pool wall, and a bottom nano-aeration network. The aeration density of the bottom nano-aeration network is 0.3-0.5m 3 / h·m 2 ; Water treatment unit: cyclone sedimentation tank, drum microfiltration machine, biofloc reactor, MBBR reactor connected in sequence; Environmental control unit: includes PID temperature control module, dissolved oxygen feedback system and pH automatic compensation device.

10. A system and method for industrialized circulating water standardization of Penaeus vannamei according to claim 1, characterized in that: The nanometer aeration pipe network is made of hydrophobic polytetrafluoroethylene membrane, with a pore size distribution of 30-50nm and an oxygen utilization rate of ≥85%.

Images