Device and method for loading freeze-drying protective agent on red blood cells

Abstract

The invention relates to a device and method for loading a freeze-drying protective additive on red blood cells and relates to the field of red blood cell treatment.The device comprises a material box and a plurality of material shelves, an opening is formed in one side of the material box, a box door is hinged to the opening, and the edge of the closed box door is connected with the material box in a sealed mode; first sliding grooves are correspondingly formed in the two opposite sides in the material box, and an open port is formed in one end of each first sliding groove so that the material frame can be in sliding connection with the first sliding grooves through the open ports of the first sliding grooves; heater assemblies are arranged on the upper and lower sides of the material rack; a vacuumizing assembly is arranged on the top of the material box, and an extraction opening of the vacuumizing assembly communicates with the geometric center positions of the two opposite sides of the material box correspondingly. A condensation assembly is arranged at the top in the material box; a containing groove is formed in the bottom of the material box. A refrigerator is fixedly arranged in the placing groove; and a controller is also arranged on the material box. The problems that in the prior art, temperature and vacuum degree control precision is low, and device setting is unreasonable are solved.

Description

Technical Field

[0001] The present invention relates to the field of red blood cell processing, and in particular to a device and method for loading red blood cells with a freeze-dried protective agent. Background Art

[0002] In the biomedical field, red blood cells are an important blood component, and their storage and processing are crucial to maintaining their biological activity and function. In particular, in the process of red blood cells loaded with lyophilization protectants, the control of environmental conditions such as temperature, vacuum, and humidity is extremely strict. This is because red blood cells are susceptible to physical and chemical damage during the freeze-drying process, such as cell rupture caused by ice crystal formation and osmotic pressure changes caused by uneven protective agent concentrations, which will seriously affect the survival rate and function of red blood cells.

[0003] Traditional red blood cell freeze-drying devices often find it difficult to meet these stringent requirements at the same time. For example, some devices heat the material unevenly and have rough temperature control capabilities, which in turn affects the survival rate of red blood cells; other devices only use single-point vacuuming, resulting in insufficient local vacuuming, which also affects the survival rate of red blood cells; in addition, the layout of the heater component and condensation component of traditional devices is not reasonable, resulting in uneven heat energy transfer, affecting the freeze-drying effect. Summary of the invention

[0004] In view of this, the present invention aims to provide a device and method for loading lyophilized protective agents on red blood cells, so as to solve the problems of low temperature and vacuum control accuracy and unreasonable device settings in the prior art.

[0005] To achieve the above object, the technical solution of the present invention is achieved as follows:

[0006] The first aspect of the present invention provides a device for loading lyophilized protective agents on red blood cells:

[0007] It comprises a material box and a plurality of material racks, wherein one side of the material box is open and a box door is hinged at the opening, and the edge of the box door is sealed and connected to the material box after closing;

[0008] First slide grooves are correspondingly provided on opposite sides of the material box, and an open port is provided at one end of the first slide groove so that the material rack is slidably connected with the first slide groove through the open port of the first slide groove;

[0009] Heater assemblies are provided on the upper and lower sides of the material rack;

[0010] The material box is provided with second slide grooves at two opposite sides thereof, and one end of the second slide groove is provided with an open port so that the heater assembly can be slidably connected with the second slide groove through the open port of the second slide groove;

[0011] A vacuum pumping assembly is disposed on the top of the material box, and the air suction ports of the vacuum pumping assembly are respectively connected to the geometric center positions of the two opposite sides of the material box;

[0012] A condensing assembly is arranged on the top of the material box; a placement groove is provided at the bottom of the material box; a refrigerator is fixedly arranged in the placement groove, and a refrigeration port communicating with the interior of the material box is longitudinally provided on the placement groove;

[0013] The material box is also provided with a controller which is respectively connected to the refrigerator, the vacuum assembly, the heater assembly and the condensation assembly.

[0014] Furthermore, the box door is provided with a transparent observation window for observing the interior of the material box.

[0015] Furthermore, a first sealing groove is provided on one side of the material box opening; a second sealing groove matching the first sealing groove is provided on the side of the box door close to the material box; and a sealing gasket is provided between the first sealing groove and the second sealing groove.

[0016] Furthermore, the heater assembly includes a connecting plate; at least one heating plate is respectively arranged on the upper and lower sides of the connecting plate;

[0017] The material box is provided with temperature sensors corresponding to the number of the heating plates, and is used to measure the temperature of the corresponding heating plates.

[0018] Furthermore, there are multiple heating plates.

[0019] Furthermore, the vacuum pump assembly includes a vacuum pump and a vacuum tube; the vacuum port of the vacuum pump is connected to the geometric center positions on two opposite sides of the material box through the vacuum tube.

[0020] Furthermore, the condensation assembly includes a condenser arranged at the top of the material box, a water receiving pan for collecting water dripping from the condenser, a drain pipe, and a water storage tank arranged on the side of the material box away from the opening of the material box, and the lowest point of the water receiving pan is connected to the drain pipe; one end of the drain pipe is connected to the lowest point of the water receiving pan, and the other end is connected to the water storage tank.

[0021] Furthermore, a bottom cover is fixed to the notch of the placement slot by bolts.

[0022] Furthermore, the refrigeration port is provided with a grille.

[0023] The second aspect of the present invention provides a method for loading erythrocytes with a lyophilization protectant, the method comprising:

[0024] The controller controls the refrigerator to cool down to a first preset temperature so as to solidify the water in the red blood cells loaded with the freeze-dried protective agent on the material rack into a solid state;

[0025] The vacuum pumping component is controlled by the controller to vacuum the inside of the material box;

[0026] Controlling the heater assembly to heat to a second preset temperature through a controller so that the water in the red blood cells sublimates into steam;

[0027] The condensation component is opened by a controller to condense the sublimated steam into liquid water and collect the liquid water.

[0028] Compared with the prior art, the present invention has the following advantages:

[0029] In the present invention, by arranging heater components on the upper and lower sides of the material rack, the temperature of the material on the material rack can be precisely controlled, avoiding the problem of uneven and rough temperature control in traditional devices, thereby reducing the physical and chemical damage to red blood cells caused by temperature changes during the freeze-drying process, thereby improving the survival rate and functional retention of red blood cells.

[0030] The present invention uses a vacuum pumping component to vacuum the material box at multiple points, so that the present invention can more evenly extract the gas in the material box, avoiding the problem of insufficient local vacuum caused by single-point vacuum pumping. The uniform vacuum distribution helps to accelerate the freeze-drying process, improve the freeze-drying efficiency, and reduce the damage to red blood cells caused by uneven vacuum.

[0031] The present invention arranges the condensation assembly at the top of the material box and arranges the heater assembly on both sides of the material rack. By rationally arranging the heater assembly, the present invention optimizes the heat transfer path and reduces heat loss. By rationally arranging the condensation assembly, the condensation efficiency of the condenser is improved. This layout not only improves the heating and condensation efficiency, but also ensures the uniformity of the temperature distribution in the material box, further improving the freeze-drying effect.

[0032] The material rack and the heater assembly in the present invention are connected to the material box through a slide slot, which is convenient for users to quickly adjust the position or replace them according to actual needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] The accompanying drawings constituting a part of the present invention are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the accompanying drawings:

[0034] Figure 1 It is a schematic diagram of the overall structure of the box door of the present invention when it is closed;

[0035] Figure 2 It is a schematic diagram of the overall structure of the first embodiment of the present invention when the cabinet door is opened;

[0036] Figure 3 For the present invention Figure 2A partial enlarged view of the middle A;

[0037] Figure 4 This is a schematic diagram of the overall structure of the second embodiment of the present invention when the door is open;

[0038] Figure 5 It is a structural schematic diagram of the condensation component of the present invention;

[0039] Figure 6 It is a rear view of the material box of the present invention when the box door is opened;

[0040] Figure 7 A bottom view of the material box of the present invention when the box door is opened;

[0041] Figure 8 It is a bottom view of the box door of the present invention when it is opened and the bottom cover is removed;

[0042] Fig. 9 is a schematic structural diagram of a heater assembly of the present invention;

[0043] Fig.10 It is a structural schematic diagram of the material rack of the present invention.

[0044] Description of reference numerals:

[0045] 1. Material box; 11. First slide; 12. Second slide; 13. Temperature sensor; 14. Bottom cover; 15. Refrigeration port; 16. First sealing groove; 17. Second sealing groove; 2. Box door; 21. Observation window; 3. Material rack; 4. Heater assembly; 41. Connecting plate; 42. Heating plate; 5. Controller; 6. Vacuum assembly; 61. Vacuum pump; 62. Vacuum tube; 7. Refrigerator; 8. Condensation assembly; 81. Condenser; 82. Water tray; 83. Drain pipe; 84. Water storage tank. DETAILED DESCRIPTION

[0046] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments may be combined with each other.

[0047] In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "upper", "lower", "inner", "back", etc. are based on the directions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0048] In addition, in the description of the present invention, unless otherwise clearly defined, the terms "installed", "connected", "connected", and "connector" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood in combination with specific circumstances.

[0049] The following will refer to the attached Figures 1 to 10 The present invention is described in detail with reference to embodiments.

[0050] Overall, if Figure 1 , Figure 2 , Figure 3 , Figure 4 as well as Fig.10 As shown, the first embodiment of the present invention provides a device for loading lyophilized protective agent on red blood cells:

[0051] It includes a material box 1 and a plurality of material racks 3. One side of the material box 1 is open and a box door 2 is hinged at the opening. When closed, the edge of the box door 2 is sealed and connected to the material box 1.

[0052] First slide grooves 11 are correspondingly provided on opposite sides of the material box 1, and an open port is provided at one end of the first slide groove 11 so that the material rack 3 is slidably connected to the first slide groove 11 through the open port of the first slide groove 11;

[0053] Heater assemblies 4 are provided on the upper and lower sides of the material rack 3;

[0054] Second chutes 12 are correspondingly provided on opposite sides of the material box 1, and an open port is provided at one end of the second chutes 12 so that the heater assembly 4 is slidably connected to the second chutes 12 through the open port of the second chutes 12;

[0055] A vacuum assembly 6 is disposed on the top of the material box 1, and the vacuum ports of the vacuum assembly 6 are respectively connected to the geometric center positions of the two opposite sides of the material box 1;

[0056] A condensing assembly 8 is provided at the top of the material box 1; a placement groove is provided at the bottom of the material box 1; a refrigerator 7 is fixedly provided in the placement groove, and a refrigeration port 15 communicating with the interior of the material box 1 is longitudinally provided on the placement groove;

[0057] The material box 1 is also provided with a controller 5 which is respectively connected to the refrigerator 7, the vacuum assembly 6, the heater assembly 4 and the condensation assembly 8.

[0058] In the present invention, by arranging heater components 4 on the upper and lower sides of the material rack 3, the temperature of the material on the material rack 3 can be precisely controlled, avoiding the problem of uneven and rough temperature control in traditional devices, thereby reducing the physical and chemical damage to red blood cells caused by temperature changes during the freeze-drying process, thereby improving the survival rate and functional retention of red blood cells.

[0059] The present invention uses the vacuum pumping assembly 6 to vacuum the material box 1 at multiple points, so that the present invention can more evenly extract the gas in the material box 1, avoiding the problem of insufficient local vacuum caused by single-point vacuuming. The uniform vacuum distribution helps to accelerate the freeze-drying process, improve the freeze-drying efficiency, and reduce the damage to red blood cells caused by uneven vacuum.

[0060] The present invention arranges the condensation assembly 8 at the top of the material box 1, and arranges the heater assembly 4 on both sides of the material rack 3. By rationally arranging the heater assembly 4, the present invention optimizes the heat transfer path and reduces heat loss. By rationally arranging the condensation assembly 8, the condensation efficiency of the condenser 81 is improved. This layout not only improves the heating and condensation efficiency, but also ensures the uniformity of the temperature distribution in the material box 1, further improving the freeze-drying effect.

[0061] The material rack 3 and the heater assembly 4 in the present invention are both connected to the material box 1 through a slide slot, so that the user can quickly adjust the position or replace them according to actual needs.

[0062] In one possible implementation, the box door 2 is provided with a transparent observation window 21 for observing the interior of the material box 1. The transparent observation window 21 allows the user to directly observe the status of the red blood cells and the lyophilization protective agent inside the material box 1 without opening the box door 2. This helps the user to promptly discover and deal with any abnormalities, such as uneven material distribution, lyophilization progress not meeting expectations, etc., thereby ensuring the smooth progress of the lyophilization process. In addition, frequently opening the box door 2 to check the status of the material may increase the risk of external contaminants entering the material box 1. Observation through the transparent observation window 21 can avoid unnecessary opening of the box door 2, thereby reducing the risk of contamination and protecting the purity and integrity of the red blood cells.

[0063] In a possible implementation, a first sealing groove 16 is provided on the opening side of the material box 1; a second sealing groove 17 cooperating with the first sealing groove 16 is provided on the side of the box door 2 close to the material box 1; and a sealing gasket is provided between the first sealing groove 16 and the second sealing groove 17. The first sealing groove 16, the second sealing groove 17 and the sealing gasket cooperate to improve the airtightness of the material box 1, thereby reducing material loss or contamination caused by poor sealing and improving product quality and stability.

[0064] In one possible implementation, Fig. 9As shown, the heater assembly 4 includes a connecting plate 41; at least one heating plate 42 is respectively arranged on the upper and lower sides of the connecting plate 41;

[0065] The material box 1 is provided with temperature sensors 13 corresponding to the number of the heating plates 42 , and used for measuring the temperature of the corresponding heating plates 42 .

[0066] In a possible implementation, there are multiple heating plates 42 .

[0067] In this embodiment, the controller 5 controls the corresponding heating plate 42 individually according to the data fed back by each temperature sensor 13, and the control algorithm is based on the PID algorithm; the individual control of multiple heating plates 42 enables the present invention to have higher temperature control accuracy.

[0068] In a possible implementation, the vacuum assembly 6 includes a vacuum pump 61 and a vacuum tube 62 ; the vacuum port of the vacuum pump 61 is connected to the geometric center positions of the opposite sides of the material box 1 through the vacuum tube 62 .

[0069] In one possible implementation, Figure 5 and Figure 6 As shown, the condensation assembly 8 includes a condenser 81 arranged at the top of the material box 1, a water receiving tray 82 for collecting water dripping from the condenser 81, a drain pipe 83, and a water storage tank 84 arranged on the material box 1 away from the opening side of the material box 1, and the drain pipe 83 is connected to the lowest point of the water receiving tray 82; one end of the drain pipe 83 is connected to the lowest point of the water receiving tray 82, and the other end is connected to the water storage tank 84.

[0070] In one possible implementation, Figure 7 and Figure 8 As shown, the notch of the placement slot is fixed with a bottom cover 14 by bolts.

[0071] The bottom cover 14 can not only be used to protect the refrigerator 7, but also the installation mode in which the bolts can be detachably assembled and assembled is convenient for maintenance.

[0072] In a possible implementation, the refrigeration port 15 is provided with a grille. The tiny holes of the grille can effectively prevent external foreign matter (such as dust, particles, etc.) from entering the interior of the material box 1.

[0073] This helps to maintain the cleanliness of the material box 1 and reduce the loss or functional decline of red blood cells caused by foreign matter contamination.

[0074] Another embodiment of the present invention provides a method for loading lyophilization protective agent on red blood cells, the method comprising:

[0075] Step S1, controlling the refrigerator 7 to cool down to a first preset temperature through the controller 5, so as to solidify the water in the red blood cells loaded with the lyophilization protective agent on the material rack 3 into a solid state;

[0076] Step S2, controlling the vacuum pumping assembly 6 to vacuum the interior of the material box 1 through the controller 5;

[0077] Step S3, controlling the heater assembly 4 to heat to a second preset temperature through the controller 5, so that the water in the red blood cells sublimates into steam;

[0078] Step S4: the controller 5 controls the condensation assembly 8 to start, condenses the sublimated steam into liquid water and collects the liquid water.

[0079] Step S4 further includes that the controller 5 controls the condenser 81 to open, the water condensed by the condenser 81 is received by the water receiving pan 82, the water flows to the lowest point of the water receiving pan 82 and flows into the water storage tank 84 through the drain pipe 83, and a sealing plug is provided at the bottom of the water storage tank 84, and the water is drained by opening the sealing plug after condensation is completed.

[0080] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A device for loading lyophilized protective agent on red blood cells, characterized in that: It comprises a material box (1) and a plurality of material racks (3); the material box (1) has an opening on one side and a box door (2) is hinged at the opening; the edge of the box door (2) is sealed and connected to the material box (1) when closed; The material box (1) has first slide grooves (11) formed on opposite sides thereof, and one end of the first slide groove (11) has an open port so that the material rack (3) is slidably connected to the first slide groove (11) through the open port of the first slide groove (11); Heater assemblies (4) are provided on the upper and lower sides of the material rack (3); The material box (1) has second slide grooves (12) formed on opposite sides thereof, and one end of the second slide groove (12) is provided with an open port so that the heater assembly (4) is slidably connected to the second slide groove (12) through the open port of the second slide groove (12); The top of the material box (1) is provided with a vacuum pumping assembly (6), and the air suction port of the vacuum pumping assembly (6) is respectively connected to the geometric center positions of two opposite sides of the material box (1); A condensing assembly (8) is arranged at the top of the material box (1); a placement groove is provided at the bottom of the material box (1); a refrigerator (7) is fixedly arranged in the placement groove, and a refrigeration port (15) communicating with the interior of the material box (1) is longitudinally provided on the placement groove; The material box (1) is also provided with a controller (5) which is respectively connected to a refrigerator (7), a vacuum pumping component (6), a heater component (4) and a condensing component (8).

2. The device for loading lyophilized protective agent on red blood cells according to claim 1, characterized in that: The box door (2) is provided with a transparent observation window (21) for observing the interior of the material box (1).

3. The device for loading lyophilized protective agent on red blood cells according to claim 1, characterized in that: A first sealing groove (16) is provided on one side of the opening of the material box (1); a second sealing groove (17) cooperating with the first sealing groove (16) is provided on the side of the box door (2) close to the material box (1); and a sealing gasket is provided between the first sealing groove (16) and the second sealing groove (17).

4. The device for loading lyophilized protective agent on red blood cells according to claim 1, characterized in that: The heater assembly (4) comprises a connecting plate (41); at least one heating plate (42) is respectively arranged on the upper and lower sides of the connecting plate (41); The material box (1) is provided with temperature sensors (13) corresponding in number to the number of the heating plates (42) for measuring the temperature of the corresponding heating plates (42).

5. The device for loading lyophilized protective agent on red blood cells according to claim 4, characterized in that: There are multiple heating plates (42).

6. The device for loading lyophilized protective agent on red blood cells according to claim 1, characterized in that: The vacuum pump assembly (6) comprises a vacuum pump (61) and a vacuum tube (62); the vacuum port of the vacuum pump (61) is connected to the geometric center positions of the two opposite sides of the material box (1) through the vacuum tube (62).

7. The device for loading lyophilized protective agent on red blood cells according to claim 1, characterized in that: The condensation assembly (8) comprises a condenser (81) arranged at the top of the material box (1), a water receiving tray (82) for receiving water dripping from the condenser (81), a drain pipe (83), and a water storage tank (84) arranged on the material box (1) at a side away from the opening of the material box (1), wherein the lowest point of the water receiving tray (82) is connected to the drain pipe (83); one end of the drain pipe (83) is connected to the lowest point of the water receiving tray (82), and the other end is connected to the water storage tank (84).

8. The device for loading lyophilized protective agent on red blood cells according to claim 1, characterized in that: The notch of the placement slot is fixed with a bottom cover (14) via bolts.

9. The device for loading lyophilized protective agent on red blood cells according to claim 1, characterized in that: The refrigeration port (15) is provided with a grille.

10. A method for loading erythrocytes with a lyophilization protectant, characterized in that: Methods include The controller (5) controls the refrigerator (7) to cool down to a first preset temperature so as to solidify the water in the red blood cells loaded with the freeze-drying protective agent on the material rack (3) into a solid state; A controller (5) controls a vacuum pumping component (6) to vacuum the interior of the material box (1); Controlling the heater assembly (4) to heat to a second preset temperature through a controller (5) so that the water in the red blood cells sublimates into steam; The controller (5) controls the condensation component (8) to open, condenses the sublimated steam into liquid water and collects the liquid water.

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