Fresh-keeping storage method of grouper

Abstract

The invention provides a method for preserving and storing groupers, which comprises the following steps of: washing iced and fresh groupers with saline water, draining, soaking in treating fluid for 25-30 minutes, taking out, air-drying the soaked groupers at 0-2 DEG C, carrying out vacuum packaging, and storing at 0-4 DEG C, the treating fluid comprises treating fluid A and treating fluid B; the treating fluid A comprises the following raw materials: clinacanthus nutans extract, polygonatum polysaccharide, peach gum powder, pectin and glycerol; the treating fluid B comprises the following raw materials: lalang grass rhizome extract, honeysuckle flower extract, sodium alginate, soya bean lecithin, carboxymethyl cellulose and butylated hydroxyanisole. In the grouper fresh-keeping storage process, a scientific and reasonable treating fluid prepared from clinacanthus nutans extract, polygonatum polysaccharide, peach gum powder, lalang grass rhizome extract, honeysuckle flower extract and other raw materials is adopted to treat chilled groupers, and a layer of uniform and stable fresh-keeping film can be formed to kill and block bacteria and microorganisms on the surfaces of grouper bodies; the freshness of the grouper meat in the refrigeration process is guaranteed, and the shelf life is prolonged.

Description

Technical Field

[0001] The invention relates to the technical field of fresh-keeping, and in particular to a method for preserving and storing grouper. Background Art

[0002] Grouper is high in unsaturated fatty acids and mineral elements, has high nutritional value, and has delicious meat. It is loved by many consumers and is one of the four famous fishes in my country. With the development of grouper breeding and aquaculture technology, the aquaculture output of grouper has increased year by year, and the demand has also increased year by year. For long-distance transportation, grouper needs to be frozen for preservation. In order to avoid the influence of freezing on grouper during short-distance transportation, most of them are kept fresh by low-temperature refrigeration. Since grouper muscle tissue contains high protein and water, it is easy to be corrupted and deteriorated by microorganisms and enzymes during storage, resulting in a short shelf life and difficulty in long-term storage. At the same time, it also affects the sensory quality of the fish. At present, grouper can be treated with plastic wrap or preservative liquid during low-temperature refrigeration. Although the shelf life can be increased, the taste and freshness of grouper are still greatly reduced, which is difficult to meet the requirements. Therefore, new grouper preservation and storage technology is needed, which is of great significance for extending the shelf life of grouper and improving product quality. Summary of the invention

[0003] In view of this, the present invention provides a method for preserving and storing grouper. The method can effectively extend the shelf life of grouper without affecting the quality of its meat.

[0004] The technical solution of the present invention is achieved in this way:

[0005] A method for preserving and storing grouper comprises the following steps:

[0006] Take the fresh grouper, rinse it with salt water, drain it, soak it in the treatment solution for 25-30 minutes, take it out, air-dry the soaked grouper at 0-2°C, vacuum pack it, and store it at 0-4°C;

[0007] The treatment liquid includes treatment liquid A and treatment liquid B;

[0008] The treatment solution A comprises the following raw materials: extract of Herba Lycopodii, Polygonatum cyrtonemae polysaccharide, peach gum powder, pectin, glycerin and water;

[0009] The treatment solution B comprises the following raw materials: Imperata root extract, honeysuckle extract, sodium alginate, soybean lecithin, carboxymethyl cellulose, butylated hydroxyanisole and water.

[0010] Furthermore, the concentration of the brine is 10-15wt%.

[0011] Furthermore, the treatment liquid includes treatment liquid A and treatment liquid B in a mass ratio of 6-9:3-5.

[0012] Furthermore, the treatment solution A comprises the following raw materials in parts by weight: 18-23 parts of Herba Lycopodii extract, 10-13 parts of Polygonatum sibiricum polysaccharide, 5-8 parts of peach gum powder, 5-8 parts of pectin, 3-4 parts of glycerin, and 48-56 parts of water.

[0013] Furthermore, the treatment solution B comprises the following raw materials in parts by weight: 20-26 parts of Imperata root extract, 20-26 parts of honeysuckle extract, 9-13 parts of sodium alginate, 5-8 parts of soybean lecithin, 2-4 parts of carboxymethyl cellulose, 0.002-0.005 parts of butylated hydroxyanisole, and 60-75 parts of water.

[0014] Furthermore, the preparation method of the A treatment liquid is: adding the Herba Lysimachiae extract and Polygonatum sibiricum polysaccharide to water while stirring and mixing evenly, then adding peach gum powder and pectin and stirring and mixing evenly, and then adding glycerin and stirring and mixing evenly, so as to obtain the A treatment liquid.

[0015] Furthermore, the stirring speed is 600-800 r / min and the time is 8-20 min.

[0016] Furthermore, the preparation method of the B treatment solution is: soybean lecithin and butylated hydroxyanisole are stirred and mixed evenly to obtain a mixed solution, and the mixture is set aside; sodium alginate and carboxymethyl cellulose are added into water and stirred and mixed evenly, and then Imperata root extract and honeysuckle extract are added and stirred and mixed evenly, and then the mixed solution is added and stirred and mixed evenly at a high speed to obtain the B treatment solution.

[0017] Furthermore, the stirring speed for uniform mixing is 400-600 r / min and the time is 10-16 min; the stirring speed for uniform high-speed stirring is 1000-1200 r / min and the time is 5-7 min.

[0018] Furthermore, the treatment liquid of the present invention is obtained by stirring and mixing treatment liquid A and treatment liquid B uniformly.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] During the preservation and storage process of grouper, the present invention uses a scientific and reasonable treatment liquid made from raw materials such as wormwood extract, polygonatum polysaccharide, peach gum powder, cogongrass root extract, honeysuckle extract, etc. to treat the chilled grouper, so that a uniform and stable preservation film can be formed on the surface of the grouper, which kills and blocks bacteria and microorganisms on the surface of the fish body, ensures the freshness of the grouper meat during the refrigeration process, and improves the shelf life of the grouper.

[0021] The present invention prepares treatment liquid A and treatment liquid B separately and then mixes the two for use, thereby ensuring the film-forming stability of treatment liquid A and treatment liquid B during use and prolonging the preservation effect. In the process of preparing treatment liquid A, after mixing the Herba Imperatae Extract and Polygonatum sibiricum Polysaccharide, the mixture is evenly mixed with peach gum powder, pectin and glycerin, which can enhance the stability of the active substances of Herba Imperatae Extract and Polygonatum sibiricum Polysaccharide, and can slowly release the fresh-keeping active substances, thereby extending the fresh-keeping period of the treatment liquid for grouper, and does not affect the meat quality of grouper. In the process of preparing treatment liquid B, by stirring and mixing evenly for multiple times, the stability of the fresh-keeping active substances in raw materials such as Imperatae Rhizome Extract and Honeysuckle Extract can also be enhanced, thereby improving the effect.

[0022] The preservation method of the invention is simple, easy to operate, and has a good preservation effect, and has broad promotion and application value. It provides a new solution for the preservation of seafood products such as grouper, helps to reduce production costs and improve economic benefits. DETAILED DESCRIPTION

[0023] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0024] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0025] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0026] Example 1

[0027] The treatment liquid of this embodiment includes treatment liquid A and treatment liquid B in a mass ratio of 8:5;

[0028] A treatment solution includes the following raw materials in parts by weight: 20 parts of Herba Achyranthis Bidentatae extract, 13 parts of Polygonatum cyrtonema polysaccharide, 6 parts of peach gum powder, 7 parts of pectin, 3 parts of glycerin, and 48 parts of water;

[0029] Treatment solution B includes the following raw materials in parts by weight: 26 parts of Imperata root extract, 24 parts of honeysuckle extract, 12 parts of sodium alginate, 6 parts of soybean lecithin, 3 parts of carboxymethyl cellulose, 0.003 parts of butylated hydroxyanisole, and 70 parts of water;

[0030] The preparation method of treatment liquid A is as follows: add the extract of Herba Achyranthis Bidens and Polygonatum cyrtonemae to water under the condition of stirring at a speed of 700 r / min, stirring for 10 minutes to mix evenly, then add peach gum powder and pectin under the condition of stirring at a speed of 800 r / min, stirring for 12 minutes to mix evenly, then add glycerin under the condition of stirring at a speed of 800 r / min, stirring for 15 minutes to mix evenly, thus obtaining treatment liquid A;

[0031] The preparation method of treatment solution B is as follows: soybean lecithin and butylated hydroxyanisole are stirred and mixed to obtain a mixed solution, which is set aside; sodium alginate and carboxymethyl cellulose are added to water and stirred for 15 minutes at a stirring speed of 400 r / min to mix evenly, then Imperata root extract and honeysuckle extract are added and stirred for 10 minutes at a stirring speed of 500 r / min to mix evenly, and then the mixed solution is added and stirred at a high speed of 1200 r / min for 6 minutes to mix evenly, thereby obtaining treatment solution B;

[0032] The method for preserving and storing grouper of the present embodiment comprises the following steps: taking the fresh grouper, washing it with 12wt% salt water, draining it, soaking it in a treatment solution for 30 minutes, taking it out, air-drying the soaked grouper at 0°C, vacuum-packing it, and storing it at 0-4°C.

[0033] Example 2

[0034] The treatment liquid of this embodiment includes treatment liquid A and treatment liquid B in a mass ratio of 9:4;

[0035] A treatment solution includes the following raw materials in parts by weight: 23 parts of Herba Achyranthis Bidentatae extract, 10 parts of Polygonatum cyrtonema polysaccharide, 5 parts of peach gum powder, 8 parts of pectin, 4 parts of glycerin, and 56 parts of water;

[0036] Treatment solution B includes the following raw materials in parts by weight: 23 parts of Imperata root extract, 20 parts of honeysuckle extract, 13 parts of sodium alginate, 5 parts of soybean lecithin, 4 parts of carboxymethyl cellulose, 0.002 parts of butylated hydroxyanisole, and 60 parts of water;

[0037] The preparation method of treatment liquid A is as follows: add the extract of Herba Achyranthis Bidens and Polygonatum cyrtonemae to water under the condition of stirring at a speed of 600 r / min, stirring for 15 minutes to mix evenly, then add peach gum powder and pectin under the condition of stirring at a speed of 600 r / min, stirring for 8-20 minutes to mix evenly, then add glycerin under the condition of stirring at a speed of 650 r / min, stirring for 20 minutes to mix evenly, thus obtaining treatment liquid A;

[0038] The preparation method of treatment solution B is as follows: soybean lecithin and butylated hydroxyanisole are stirred and mixed to obtain a mixed solution, which is set aside; sodium alginate and carboxymethyl cellulose are added into water and stirred for 10 minutes at a stirring speed of 500 r / min to mix evenly, then Imperata root extract and honeysuckle extract are added and stirred for 15 minutes at a stirring speed of 600 r / min to mix evenly, and then the mixed solution is added and stirred at a high speed of 1100 r / min for 7 minutes to mix evenly, thereby obtaining treatment solution B;

[0039] The preservation and storage method of the grouper of the present embodiment comprises the following steps: taking the fresh ice grouper, washing it with 15wt% salt water and then draining it, then soaking it in a treatment solution for 25 minutes and then taking it out, air-drying the soaked grouper at 2°C, and then vacuum-packing it and storing it at 0-4°C.

[0040] Example 3

[0041] The treatment liquid of this embodiment includes treatment liquid A and treatment liquid B in a mass ratio of 6:3;

[0042] A treatment solution includes the following raw materials in parts by weight: 18 parts of Herba Achyranthis Bidentatae extract, 12 parts of Polygonatum cyrtonema polysaccharide, 8 parts of peach gum powder, 6 parts of pectin, 3 parts of glycerin, and 50 parts of water;

[0043] Treatment solution B includes the following raw materials in parts by weight: 20 parts of Imperata root extract, 26 parts of honeysuckle extract, 9 parts of sodium alginate, 8 parts of soybean lecithin, 2 parts of carboxymethyl cellulose, 0.005 parts of butylated hydroxyanisole, and 65 parts of water;

[0044] The preparation method of treatment liquid A is as follows: add the extract of Herba Achyranthis Bidens and Polygonatum cyrtonemae to water under the condition of stirring at a speed of 800 r / min, stirring for 12 minutes to mix evenly, then add peach gum powder and pectin under the condition of stirring at a speed of 700 r / min, stirring for 15 minutes to mix evenly, then add glycerin under the condition of stirring at a speed of 7000 r / min, stirring for 10 minutes to mix evenly, thus obtaining treatment liquid A;

[0045] The preparation method of treatment solution B is as follows: soybean lecithin and butylated hydroxyanisole are stirred and mixed to obtain a mixed solution, which is set aside; sodium alginate and carboxymethyl cellulose are added into water and stirred for 12 minutes at a stirring speed of 600 r / min to mix evenly, then Imperata root extract and honeysuckle extract are added and stirred for 14 minutes at a stirring speed of 600 r / min to mix evenly, and then the mixed solution is added and stirred at a high speed of 1000 r / min for 5 minutes to mix evenly, thereby obtaining treatment solution B;

[0046] The method for preserving and storing grouper of the present embodiment comprises the following steps: taking the fresh grouper, washing it with 10wt% salt water, draining it, soaking it in a treatment solution for 25 minutes, taking it out, air-drying the soaked grouper at 1°C, vacuum-packing it, and storing it at 0-4°C.

[0047] Comparative Example 1

[0048] In this comparative example, the raw material ratio of the treatment liquid A and the treatment liquid B is adjusted on the basis of Example 1, and the rest remains the same as Example 1.

[0049] The A treatment solution of this comparative example includes the following raw materials in parts by weight: 5 parts of Herba Imperatae Extract, 29 parts of Polygonatum sibiricum Polysaccharide, 6 parts of Peach Gum Powder, 2 parts of Pectin, 7 parts of Glycerin, and 48 parts of Water; the B treatment solution includes the following raw materials in parts by weight: 6 parts of Imperatae cylindrica Rhizome Extract, 38 parts of Honeysuckle Flos Extract, 5 parts of Sodium Alginate, 14 parts of Soybean Lecithin, 8 parts of Carboxymethyl Cellulose, 0.003 parts of Butylated Hydroxyanisole, and 70 parts of Water.

[0050] Comparative Example 2

[0051] Compared with Example 1, the treatment solution A of this comparative example does not contain the extract of Herba Lycopodii, and the rest is the same as that of Example 1.

[0052] The treatment solution A of this comparative example comprises the following raw materials in parts by weight: 18 parts of polygonatum polysaccharide, 11 parts of peach gum powder, 12 parts of pectin, 8 parts of glycerin, and 48 parts of water.

[0053] Comparative Example 3

[0054] Compared with Example 1, the treatment solution B of this comparative example does not contain the Imperata cylindrica root extract, and the rest is the same as Example 1.

[0055] The treatment solution B of this comparative example includes the following raw materials in parts by weight: 32 parts of honeysuckle extract, 18 parts of sodium alginate, 12 parts of soybean lecithin, 9 parts of carboxymethyl cellulose, 0.003 parts of butylated hydroxyanisole, and 70 parts of water.

[0056] Comparative Example 4

[0057] Compared with Example 1, the present comparative example does not prepare treatment solution A and treatment solution B separately, and the rest remains the same as Example 1.

[0058] The preparation method of the treatment liquid of this comparative example is: mix the Herba Imperatae Extract, Polygonatum sibiricum Polysaccharide, peach gum powder, pectin, Imperatae cylindrica Rhizome Extract, Honeysuckle Flos Extract, sodium alginate, soybean lecithin, carboxymethyl cellulose and butylated hydroxyanisole evenly, add the mixture into water while stirring at a speed of 200 r / min, stir and mix evenly, then add glycerol and stir and mix evenly again to obtain the treatment liquid.

[0059] Comparative Example 5

[0060] Compared with Example 1, the treatment liquid of this comparative example only contains treatment liquid A, and the rest remains the same as Example 1.

[0061] Comparative Example 6

[0062] Compared with Example 1, the treatment solution of this comparative example only contains treatment solution B, and the rest remains the same as Example 1.

[0063] After the grouper was preserved and stored for a certain period of time according to the methods of the above embodiments 1-3 and comparative examples 1-6, the quality indicators of the grouper were measured. The experimental process and results are as follows.

[0064] Six professionals were selected to conduct sensory evaluation and scoring on the grouper after it had been stored for a certain period of time according to the sensory evaluation standards in the table below. The maximum score for each item was 4 points, and the results were averaged.

[0065] Table 1 Sensory evaluation standards for grouper storage

[0066]

[0067] Table 2 Sensory evaluation results

[0068] Day 4 Day 8 Day 12 Example 1 12.0 11.0 9.5 Example 2 11.8 10.5 9.2 Example 3 12.0 10.0 9.0 Comparative Example 1 11.5 10.7 8.5 Comparative Example 2 11.0 9.8 7.6 Comparative Example 3 11.2 10.2 8.2 Comparative Example 4 10.7 9.3 7.3 Comparative Example 5 10.2 9.0 6.8 Comparative Example 6 10.3 9.2 6.3

[0069] It can be seen from Table 2 that, compared with Comparative Examples 1-6, the fresh-keeping storage method of Examples 1-3 of the present invention can still maintain good sensory quality for 12 days.

[0070] On the 12th day, the total colony count and volatile basic nitrogen (TVB-N) of the grouper treated by the fresh-keeping storage methods of Examples 1-3 and Comparative Examples 1-6 were measured. The results are shown in Table 3 below.

[0071] Total colony count: Determined in accordance with the national standard GB 4789.2-2022 "National Food Safety Standard Food Microbiological Examination Determination of Total Colony Count".

[0072] Volatile basic nitrogen (TVB-N): Determined according to the Kjeldahl method in the national standard GB 5009.228-2016 "National Food Safety Standard Determination of Volatile Basic Nitrogen in Food".

[0073] Table 3 Performance indexes of grouper after 12 days of storage

[0074] Total colony count (cfu / g) TVBN value (mg / 100g) Example 1 <![CDATA[1.25×10 7 ]]> 16.37 Example 2 <![CDATA[1.38×10 7 ]]> 17.25 Example 3 <![CDATA[1.61×10 7 ]]> 17.83 Comparative Example 1 <![CDATA[3.46×10 7 ]]> 20.54 Comparative Example 2 <![CDATA[2.07×10 7 ]]> 23.70 Comparative Example 3 <![CDATA[6.72×10 7 ]]> 21.86 Comparative Example 4 <![CDATA[3.24×10 8 ]]> 25.43 Comparative Example 5 <![CDATA[6.08×10 8 ]]> 27.16 Comparative Example 6 <![CDATA[5.62×10 8 ]]> 26.09

[0075] The above results show that the present invention can effectively inhibit the growth of bacteria and the production of volatile basic nitrogen during the storage of grouper by preparing the treatment liquid made of raw materials such as the extract of the herb, polygonatum polysaccharide, peach gum powder, white imperata root extract, and honeysuckle extract in a suitable manner, and maintaining the good sensory quality of the grouper meat. Compared with Example 1 of the present invention, the various raw material components in the treatment liquid in Comparative Example 1 cannot play a good synergistic role due to the appropriate raw material ratio, and the preservation effect is weakened. Compared with Example 1, the A treatment liquid of Comparative Example 2 does not contain the extract of the herb, and the B treatment liquid of Comparative Example 3 does not contain the white imperata root extract, which also affects the preservation and storage effect of grouper, indicating that adding the extract of the herb or the white imperata root extract to the treatment liquid can improve the preservation effect of the treatment liquid. Compared with Example 1, Comparative Example 4 changed the preparation method of the treatment liquid, resulting in the treatment liquid failing to form a uniform and stable protective film on the surface of the grouper, reducing the preservation effect. Compared with Example 1, the processing liquid of Comparative Example 5 contains only processing liquid component A, and the processing liquid of Comparative Example 6 contains only processing liquid component B, and the preservation effect is reduced, indicating that the combination of processing liquid A and processing liquid B can effectively delay the spoilage rate of grouper during storage, has obvious antibacterial and preservation effects, thereby extending the shelf life of grouper.

[0076] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preserving and storing grouper, characterized in that: The following steps are involved: Take the fresh grouper, rinse it with salt water, drain it, soak it in the treatment solution for 25-30 minutes, take it out, air-dry the soaked grouper at 0-2°C, vacuum pack it, and store it at 0-4°C; The treatment liquid includes treatment liquid A and treatment liquid B; The treatment solution A comprises the following raw materials: extract of Herba Lycopodii, Polygonatum cyrtonemae polysaccharide, peach gum powder, pectin, glycerin and water; The treatment solution B comprises the following raw materials: Imperata root extract, honeysuckle extract, sodium alginate, soybean lecithin, carboxymethyl cellulose, butylated hydroxyanisole and water.

2. The method for preserving and storing grouper according to claim 1, characterized in that: The concentration of the brine is 10-15 wt %.

3. The method for preserving and storing grouper according to claim 1, characterized in that: The treatment liquid includes treatment liquid A and treatment liquid B in a mass ratio of 6-9:3-5.

4. The method for preserving and storing grouper according to claim 1, characterized in that: The treatment solution A comprises the following raw materials in parts by weight: 18-23 parts of Herba Lycopodii extract, 10-13 parts of Polygonatum cyrtonema polysaccharide, 5-8 parts of peach gum powder, 5-8 parts of pectin, 3-4 parts of glycerin, and 48-56 parts of water.

5. The method for preserving and storing grouper according to claim 1, characterized in that: The treatment solution B comprises the following raw materials in parts by weight: 20-26 parts of Imperata root extract, 20-26 parts of honeysuckle extract, 9-13 parts of sodium alginate, 5-8 parts of soybean lecithin, 2-4 parts of carboxymethyl cellulose, 0.002-0.005 parts of butylated hydroxyanisole, and 60-75 parts of water.

6. The method for preserving and storing grouper according to any one of claims 1 to 3, characterized in that: The preparation method of the A treatment liquid is as follows: add the Herba Euphorbiae extract and Polygonatum sibiricum polysaccharide into water while stirring and mixing evenly, then add peach gum powder and pectin and stir and mix evenly, and then add glycerin and stir and mix evenly to obtain the A treatment liquid.

7. The method for preserving and storing grouper according to claim 5, characterized in that: The stirring speed is 600-800 r / min and the time is 8-20 min.

8. The method for preserving and storing grouper according to any one of claims 1 to 2 or 4, characterized in that: The preparation method of the B treatment solution is as follows: soybean lecithin and butylated hydroxyanisole are stirred and mixed to obtain a mixed solution for use; sodium alginate and carboxymethyl cellulose are added into water and stirred and mixed to obtain a mixed solution; then, Imperata root extract and honeysuckle extract are added and stirred and mixed to obtain a mixed solution; and then, the mixed solution is added and stirred and mixed to obtain a B treatment solution.

9. The method for preserving and storing grouper according to claim 8, characterized in that: The stirring speed for uniform mixing is 400-600 r / min and the time is 10-16 min; the stirring speed for uniform high-speed stirring is 1000-1200 r / min and the time is 5-7 min.

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