Moisturizing composition for promoting expression of aquaporin and cosmetic application of moisturizing composition

Abstract

The invention discloses a moisturizing composition for promoting expression of aquaporin and cosmetic application of the moisturizing composition, and relates to the technical field of cosmetics. The moisturizing composition for promoting aquaporin expression is prepared from glycosylglycerol and trehalose which are sourced from blue-green algae in a mass ratio of (1-10): (1-5). When the composition is prepared by matching the glycosylglycerol derived from blue-green algae with the trehalose, the composition has remarkable advantages in improving the concentration of aquaporin and preserving moisture; the composition has a very obvious promotion effect on the aspects of skin cell aquaporin expression, skin barrier and deep skin moisturizing. If any substance is lost or the dosage of the substance is reduced, the effect becomes poor.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a moisturizing composition for promoting the expression of aquaporin and a cosmetic application thereof. Background Art

[0002] At present, the classic idea for products that enhance the skin barrier and moisturize is to add ingredients such as plant oils (such as jojoba oil and shea butter), animal oils (such as lanolin) and petrolatum (such as mineral oil and vaseline) to form a layer of oil film on the skin surface to prevent water evaporation; use ingredients such as polyols and sodium pyrrolidone carboxylate that can absorb water from the surrounding environment to increase the water content of the skin; use ingredients such as hyaluronic acid that can form a network structure to bind free water molecules into their network to prevent water from evaporating; or use ingredients such as squalane and ceramide glue, which can penetrate into the skin epidermis and repair the water absorption capacity and barrier structure of the stratum corneum. However, long-term use of overly greasy moisturizing products may cause pores to become clogged, increase the burden on the skin, and even cause skin problems such as folliculitis; in an environment with low relative humidity, hygroscopic agents may absorb water from the skin, causing the skin to become drier; it may also cause irritation or allergic reactions to the skin. The hydration and moisturizing mechanism is relatively simple, and it is impossible to seal moisture and moisturize for a long time, so a variety of moisturizers must be used in combination.

[0003] At present, people have higher and higher requirements for cosmetics. In order to meet the growing needs of consumers, the development of pure natural cosmetics has become a hot topic. Exploring the synergistic effects of different plant extracts in moisturizing and enhancing the skin barrier has become a problem that needs to be solved.

[0004] In view of this, the present invention is proposed. Summary of the invention

[0005] The object of the present invention is to provide a moisturizing composition for promoting the expression of aquaporin and its cosmetic application.

[0006] The present invention is achieved in that:

[0007] In a first aspect, the present invention provides a moisturizing composition for promoting the expression of aquaporin, which comprises, by mass percentage, 1-10 parts of cyanobacteria-derived glycerol glucoside and 1-5 parts of trehalose.

[0008] In an alternative embodiment, the cyanobacteria is Synechocystis.

[0009] In an alternative embodiment, the Synechocystis sp. is cultured in a culture medium containing a salt component.

[0010] In an alternative embodiment, the concentration of the salt component in the culture medium is 3-4%.

[0011] In an optional embodiment, the method for preparing the cyanobacteria-derived glycerol glucoside comprises:

[0012] (1) collecting and washing the algal mud obtained by culturing Synechocystis in a medium containing salt components;

[0013] (2) reducing the water content of the Synechocystis algae mud to 50% solid content, adding 1-5 mL of mixed biological enzyme per liter, and performing enzymolysis at 55-65° C. for 5-8 hours to obtain a preliminary enzymolysis solution;

[0014] (3) pouring the preliminary enzymatic hydrolysate into a high temperature and high pressure reactor, adding 2-3 times the volume of 1-2% hydrogen peroxide aqueous solution per liter of the preliminary enzymatic hydrolysate, oxidizing under high temperature and high pressure reaction conditions, the oxidation reaction time is 2-3 hours, and obtaining an oxidation product;

[0015] (4) pressing and filtering the oxidation product to obtain a clear liquid rich in small molecular organic carbon source active substances;

[0016] (5) using activated carbon adsorption, and then using 45%-60% ethanol concentration (v / v) eluent to separate and elute the glycerol glucoside to obtain a glycerol glucoside solution;

[0017] (6) Using a nanofiltration membrane to filter and separate the glycerol glucoside solution to collect the glycerol glucoside product.

[0018] In an optional embodiment, the mixed biological enzyme comprises cellulase, pectinase, glucose oxidase and acid protease in a mass ratio of 1-5:1-5:1-2:0.5-1;

[0019] And / or, the high temperature and high pressure reaction conditions have a temperature of 200-300° C. and a pressure of 9-10 MPa.

[0020] In a second aspect, the present invention provides use of the moisturizing composition for promoting the expression of aquaporin as described in any one of the aforementioned embodiments in the preparation of cosmetics.

[0021] In a third aspect, the present invention provides a moisturizing essence, comprising the moisturizing composition for promoting the expression of aquaporins as described in any one of the aforementioned embodiments, wherein the mass percentage of the moisturizing composition for promoting the expression of aquaporins in the moisturizing essence is 1-3%.

[0022] In an optional embodiment, the ingredients of the moisturizing essence also include moisturizers, chelating agents, thickeners, preservatives, emollients, emulsifiers, fragrances and water;

[0023] Preferably, based on the weight percentage of the moisturizing essence, the moisturizer is 1-3% glycerol; the chelating agent is 0.02-0.05% EDTA-2Na; the thickener is 0.5-1% ammonium acryloyldimethyltaurate / VP copolymer; the preservative is 0.2-0.5% phenoxyethanol; the emollient is 3-5% caprylic / capric triglyceride; the emulsifier is 0.4-0.6% polyglyceryl-10 stearate; the fragrance is 0.001-0.003% essence; the balance is water.

[0024] In an optional embodiment, the preparation method of the moisturizing essence comprises:

[0025] (1) mixing the moisturizer, the chelating agent, the thickener, the emollient, the emulsifier and the water at a temperature of 80 to 85° C., stirring until completely dissolved, and sterilizing by heat preservation to obtain a phase A mixture, which is then kept warm;

[0026] (2) stirring and heating the moisturizing composition for promoting the expression of aquaporin and the fragrance at 80-85° C. to completely dissolve to obtain a phase B mixture, and keeping the mixture warm;

[0027] (3) adding the phase A mixture to the phase B mixture under homogenous stirring, homogenizing, stirring and keeping warm, and then starting to cool;

[0028] (4) After the temperature drops to 60° C., add the preservative, stir evenly and serve.

[0029] The present invention has the following beneficial effects:

[0030] When the present invention uses glycerol glucoside and trehalose from cyanobacteria to prepare a composition, it has significant advantages in increasing the concentration of aquaporin and moisturizing; it has a very obvious promoting effect on the expression of aquaporin in skin cells, the skin barrier and the deep moisturizing of the skin. The lack of any one substance or the reduction in the amount will lead to a poor effect. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0032] The invention provides a moisturizing composition for promoting the expression of aquaporin, which comprises 1-10 parts of glycerol glucoside derived from cyanobacteria and 1-5 parts of trehalose in terms of mass percentage.

[0033] Glucosylglycerol (GG) is a glycoside compound synthesized by some stressed microorganisms to protect themselves. It has the ability to stabilize protein structure, activate and promote cell regeneration, enhance cell vitality, accelerate cell metabolism, enhance cell antioxidant capacity, and promote collagen synthesis.

[0034] Trehalose is extracted from marine algae such as kelp. It can resist ultraviolet stimulation and protect the skin. It also has antioxidant effects. It can neutralize and eliminate free radicals in the body, reduce the damage caused by oxidative stress, and delay cell aging.

[0035] Glycerol glucoside can be synthesized by various green plants (such as lily and mirabilis), algae (such as various cyanobacteria) and some heterotrophic microorganisms. In the present invention, glycerol glucoside derived from cyanobacteria has a better effect.

[0036] Specifically, the cyanobacteria in the present invention is Synechocystis sp., which is cultured in a medium containing salt components, wherein the concentration of the salt components in the medium is 3-4%. Salt stress can promote the production of glycerol glucoside by Synechocystis sp., thereby increasing the subsequent glycerol glucoside content.

[0037] The preparation method of glycerol glucoside from cyanobacteria includes:

[0038] (1) collecting and washing the algal mud obtained by culturing Synechocystis in a medium containing salt components;

[0039] (2) reducing the moisture content of the Synechocystis algae mud to 50% solid content, adding 1-5 mL of mixed biological enzymes per liter, wherein the mixed biological enzymes include cellulase, pectinase, glucose oxidase and acid protease in a mass ratio of 1-5:1-5:1-2:0.5-1; performing enzymolysis at 55-65° C. for 5-8 hours to obtain a preliminary enzymolysis solution;

[0040] (3) pouring the preliminary enzymatic hydrolysate into a high temperature and high pressure reactor, adding 2-3 times the volume of 1-2% hydrogen peroxide aqueous solution per liter of the preliminary enzymatic hydrolysate, and oxidizing under high temperature and high pressure reaction conditions, wherein the temperature under the high temperature and high pressure reaction conditions is 200-300° C., the pressure is 9-10 MPa, and the oxidation reaction time is 2-3 hours to obtain an oxidation product;

[0041] (4) pressing and filtering the oxidation product to obtain a clear liquid rich in small molecular organic carbon source active substances;

[0042] (5) using activated carbon adsorption, and then using 45%-60% ethanol concentration (v / v) eluent to separate and elute the glycerol glucoside to obtain a glycerol glucoside solution;

[0043] (6) Using a nanofiltration membrane to filter and separate the glycerol glucoside solution, and collect the glycerol glucoside product.

[0044] In the present invention, the glycerol glucoside in Synechocystis is extracted by the method of "salt stress + enzymolysis + high temperature and high pressure oxidation", so that a higher content of glycerol glucoside can be obtained.

[0045] In the present invention, glycerol glucoside and trehalose derived from cyanobacteria are mixed in a specific ratio, and the obtained moisturizing composition for promoting the expression of aquaporin can promote the expression of aquaporin, enhance the skin barrier and enhance the moisturizing performance.

[0046] Aquaporin (Aquaporin, AQP for short) is a protein located on the cell membrane. Like the water pump of the cell, they form "pores" on the cell membrane to control the transmembrane transport of water molecules. Aquaporin is highly selective, mainly allowing water molecules to pass quickly and efficiently, and plays an important role in maintaining the balance of water inside and outside the cell, regulating cell volume and fluid balance, etc. In the skin, AQP3 is mainly expressed, which, in addition to participating in skin hydration and skin barrier function, can also regulate the proliferation, migration and early differentiation of keratinocytes, and plays a key role in maintaining skin morphology, moisturizing, and wound healing. The moisturizing composition for promoting the expression of aquaporin provided by the present invention can play a significant effect of promoting the expression of aquaporin, and it can significantly enhance the barrier function of the skin and improve the moisturizing effect.

[0047] Therefore, the moisturizing composition for promoting the expression of aquaporin can be widely used in the preparation of cosmetics.

[0048] Specifically, the present invention provides a typical but non-limiting cosmetic example: moisturizing essence.

[0049] The ingredients of the moisturizing essence include the moisturizing composition for promoting the expression of aquaporin, and the mass percentage of the moisturizing composition for promoting the expression of aquaporin in the moisturizing essence is 1-3%.

[0050] Furthermore, the ingredients of the moisturizing essence also include humectants, chelating agents, thickeners, preservatives, emollients, emulsifiers, fragrances and water;

[0051] Preferably, based on the weight percentage of the moisturizing essence, the moisturizer is 1-3% glycerol; the chelating agent is 0.02-0.05% EDTA-2Na; the thickener is 0.5-1% ammonium acryloyldimethyltaurate / VP copolymer; the preservative is 0.2-0.5% phenoxyethanol; the emollient is 3-5% caprylic / capric triglyceride; the emulsifier is 0.4-0.6% polyglyceryl-10 stearate; the fragrance is 0.001-0.003% essence; and the balance is water.

[0052] The preparation method of the moisturizing essence comprises:

[0053] (1) mixing a moisturizer, a chelating agent, a thickener, an emollient, an emulsifier and water at a temperature of 80 to 85° C., stirring until completely dissolved, and sterilizing by heat preservation to obtain a phase A mixture, which is then kept warm;

[0054] (2) stirring and heating the moisturizing composition for promoting the expression of aquaporin and the fragrance at 80-85° C. to completely dissolve to obtain a phase B mixture, and keeping the mixture warm;

[0055] (3) Add the phase A mixture to the phase B mixture while stirring, homogenize, stir and keep warm, then start cooling;

[0056] (4) After the temperature drops to 60°C, add preservatives, stir evenly and serve.

[0057] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0058] Example 1

[0059] This embodiment provides a cyanobacteria-derived glycerol glucoside, which is extracted from Synechocystis and cultured in a BG11 culture medium containing 3.5% sodium chloride solution.

[0060] Specifically, the preparation method of glycerol glucoside derived from cyanobacteria includes:

[0061] 1) collecting and washing the algae mud of Synechocystis cultured in BG11 medium containing 3.5% sodium chloride solution;

[0062] 2) reducing the water content in the algae mud to 50% solid content, adding 1-5 mL of mixed biological enzymes (cellulase, pectinase, glucose oxidase and acid protease in a ratio of 2:2:1:0.5) per liter, and performing enzymolysis at 55-65°C for 6 hours to obtain a preliminary enzymolysis solution;

[0063] 3) pouring the preliminary enzymatic hydrolysate into a high temperature and high pressure reactor, adding 2 times the volume of 2% hydrogen peroxide aqueous solution per liter of the preliminary enzymatic hydrolysate, oxidizing under high temperature and high pressure reaction conditions of 200° C. and 10 MPa, the oxidation reaction time is 3 hours, and obtaining an oxidation product;

[0064] 4) The oxidized product is squeezed and filtered to obtain a clear liquid rich in small molecular organic carbon source active substances;

[0065] 5) using activated carbon adsorption and then using 55% ethanol concentration (v / v) as an eluent to separate and elute the glycerol glucoside;

[0066] 6) Using a nanofiltration membrane to filter and separate the glycerol glucoside solution, and collecting the glycerol glucoside product.

[0067] The glycerol glucoside extracted in Example 1 was mixed evenly with trehalose in different proportions to obtain the moisturizing compositions for promoting the expression of aquaporins in Examples 2-13.

[0068] Example 2

[0069] This embodiment provides a moisturizing composition for promoting the expression of aquaporin. The moisturizing composition for promoting the expression of aquaporin has a total mass of 10 g, and includes glycerol glucoside and trehalose derived from cyanobacteria in a mass ratio of 1:1.

[0070] Example 3

[0071] This embodiment provides a moisturizing composition for promoting the expression of aquaporin. The moisturizing composition for promoting the expression of aquaporin has a total mass of 10 g, and comprises glycerol glucoside and trehalose derived from cyanobacteria in a mass ratio of 1:2.

[0072] Example 4

[0073] This embodiment provides a moisturizing composition for promoting the expression of aquaporin. The moisturizing composition for promoting the expression of aquaporin has a total mass of 10 g, and comprises glycerol glucoside and trehalose derived from cyanobacteria in a mass ratio of 1:3.

[0074] Example 5

[0075] This embodiment provides a moisturizing composition for promoting the expression of aquaporin. The moisturizing composition for promoting the expression of aquaporin has a total mass of 10 g, and includes glycerol glucoside and trehalose derived from cyanobacteria in a mass ratio of 1:5.

[0076] Example 6

[0077] This embodiment provides a moisturizing composition for promoting the expression of aquaporin. The moisturizing composition for promoting the expression of aquaporin has a total mass of 10 g, and comprises glycerol glucoside and trehalose derived from cyanobacteria in a mass ratio of 2:1.

[0078] Example 7

[0079] This embodiment provides a moisturizing composition for promoting the expression of aquaporin. The moisturizing composition for promoting the expression of aquaporin has a total mass of 10 g, and includes glycerol glucoside and trehalose derived from cyanobacteria in a mass ratio of 3:1.

[0080] Example 8

[0081] This embodiment provides a moisturizing composition for promoting the expression of aquaporin. The moisturizing composition for promoting the expression of aquaporin has a total mass of 10 g, and includes glycerol glucoside and trehalose derived from cyanobacteria in a mass ratio of 5:1.

[0082] Example 9

[0083] This embodiment provides a moisturizing composition for promoting the expression of aquaporin. The moisturizing composition for promoting the expression of aquaporin has a total mass of 10 g, and includes glycerol glucoside and trehalose derived from cyanobacteria in a mass ratio of 7:1.

[0084] Example 10

[0085] This embodiment provides a moisturizing composition for promoting the expression of aquaporin. The moisturizing composition for promoting the expression of aquaporin has a total mass of 10 g, and includes glycerol glucoside and trehalose derived from cyanobacteria in a mass ratio of 10:1.

[0086] Embodiment 11

[0087] This embodiment provides a moisturizing composition for promoting the expression of aquaporin. The moisturizing composition for promoting the expression of aquaporin has a total mass of 10 g, and includes 3 parts of glycerol glucoside derived from cyanobacteria and 2 parts of trehalose in a mass ratio of 3:2.

[0088] Example 12

[0089] This embodiment provides a moisturizing composition for promoting the expression of aquaporin. The moisturizing composition for promoting the expression of aquaporin has a total mass of 10 g, and includes glycerol glucoside and trehalose derived from cyanobacteria in a mass ratio of 5:2.

[0090] Example 13

[0091] This embodiment provides a moisturizing composition for promoting the expression of aquaporin. The moisturizing composition for promoting the expression of aquaporin has a total mass of 10 g, and includes glycerol glucoside and trehalose derived from cyanobacteria in a mass ratio of 6:1.

[0092] Comparative Example 1

[0093] This comparative example contains only 10 g of glycerol glucoside derived from cyanobacteria, but does not contain trehalose.

[0094] Comparative Example 2

[0095] This comparative example does not contain glycerol glucoside derived from cyanobacteria, but only contains 10 g of trehalose.

[0096] Comparative Example 3

[0097] This comparative example provides a moisturizing composition, which comprises glycerol glucoside and trehalose in a mass ratio of 1:1, based on a total mass of 10 g of the moisturizing composition, wherein the glycerol glucoside is obtained by extracting from other cyanobacteria (Anabaena) according to the method of Example 1.

[0098] Comparative Example 4

[0099] This comparative example provides a moisturizing composition for promoting the expression of aquaporin. The total mass of the moisturizing composition for promoting the expression of aquaporin is 10 g, and the composition comprises glycerol glucoside and trehalose of cyanobacteria origin in a mass ratio of 1:1. The difference between this comparative example and Example 2 is that the step of high temperature and high pressure oxidation of the preliminary enzymatic hydrolysate in Example 1 is omitted for the glycerol glucoside of cyanobacteria origin in this comparative example, and the preliminary enzymatic hydrolysate is directly subjected to the treatment of steps (4)-(6).

[0100] Test Example 1: Determination of polysaccharide content

[0101] Samples were taken from the products obtained in Examples 2-13 and Comparative Examples 1-4, and the total sugar content in the samples was determined as follows:

[0102] (1) Glucose standard solution

[0103] Accurately weigh 10 mg of anhydrous glucose reference substance, place it in a 100 mL volumetric flask, add appropriate amount of distilled water to dissolve, dilute to the scale, and shake well. At this time, the glucose concentration is 0.1 mg / mL.

[0104] (2) Standard curve

[0105] Take 0, 100, 200, 400, 600, 800, 1000 μL of the above-mentioned reference solution, place them in test tubes respectively, dilute to 1 mL, continue to add 1 mL of 5% phenol solution (prepared immediately before use), shake well, quickly add 5 mL of concentrated sulfuric acid (add slowly), shake well, keep warm in a boiling water bath for 10-15 min, take out, cool to room temperature, use the corresponding reagent as a blank control, measure the absorbance at a wavelength of 488 nm according to the UV-Vis spectrophotometry, and draw a standard curve with the absorbance value as the ordinate and the glucose concentration as the abscissa.

[0106] (3) Determination of polysaccharide content in samples

[0107] Pipette 1 mL of sample into a test tube, add 1 mL of distilled water, and follow the same steps as the standard curve determination to determine the optical density of the sample. Calculate the polysaccharide content of the sample.

[0108] Table 1. Polysaccharide content test results of each sample

[0109]

[0110]

[0111] It can be seen from Table 1 above that there is a significant difference in the total sugar content between Examples 2-13 and Comparative Examples 1-4. The specific reason is that both glycerol glucoside and trehalose are glycoside compounds, and the lack of glycerol glucoside or trehalose from cyanobacteria will lead to a decrease in the total sugar content of the prepared composition. And the combination of the two in a specific ratio has a synergistic effect. When other cyanobacteria are selected to extract glycerol glucoside, the total sugar content obtained is significantly lower than that of Example 2, and in the process of extracting glycerol glucoside, if the high temperature and high pressure oxidation step is omitted, the content of glycerol glucoside will also be reduced, and the total sugar content obtained will be significantly lower than that of Example 2.

[0112] Test Example 2: Cytotoxicity Detection

[0113] (1) Preparation of cell suspension: Digest HaCat cells with 2 mL of 0.05% trypsin for 2 min, then add 4 mL of fresh MEM complete medium to terminate the enzymatic hydrolysis. Centrifuge, remove the supernatant, add 1 mL of fresh MEM complete medium, resuspend the cells, and finally count them using a hemocytometer. Inoculate into a 96-well plate and plate according to the appropriate number of cells (approximately 1×10 4 Each well was filled with about 100 μL of cell suspension. After the cells were inoculated, they were placed in a 37°C incubator and cultured until the confluence reached 70-80%, and then used for cytotoxicity testing.

[0114] (2) After removing the culture medium, the mixture was washed once with PBS, and the samples prepared in Examples 2-13 and Comparative Examples 1-4 were added. Three replicates were set for each sample, and the mixture was placed in a 37° C. incubator for 24 hours.

[0115] (3) Add MTT solution: Add 10 μL of 5 mg / mL MTT solution to each well and continue incubation for 4 hours. Remove the supernatant and add 100 μL of DMSO to each well. Incubate in a 37°C incubator for 15 minutes to allow the crystals to fully dissolve.

[0116] (4) Use an enzyme-linked immunosorbent assay (ELISA) to measure the absorbance of each well at 490 nm.

[0117] (5) Analyze data.

[0118] Table 2. Cell viability test results of each sample

[0119] sample Cell survival rate / % Control 100％ Example 2 112.44％ Example 3 113.86％ Example 4 116.10％ Example 5 121.44％ Example 6 122.14％ Example 7 101.92％ Example 8 106.36％ Example 9 109.22％ Example 10 112.95％ Embodiment 11 134.92％ Example 12 143.55％ Example 13 124.11％ Comparative Example 1 103.26％ Comparative Example 2 101.25％ Comparative Example 3 96.54％ Comparative Example 4 102.38％

[0120] It can be seen from Table 2 that: According to the analysis of the experimental results, the samples prepared in Examples 2-13 and Comparative Examples 1-2 and 4 did not show obvious cytotoxicity after being diluted 10 times, and they were able to promote cell proliferation. However, since Comparative Example 3 was extracted with Anabaena, its cell survival rate was lower than that of the blank group, indicating that it had certain cytotoxicity.

[0121] Test Example 3: Determination of the efficacy of promoting aquaporin

[0122] Samples were taken from the products obtained in Examples 2-13 and Comparative Examples 1-4, and the samples were tested for their efficacy in promoting aquaporin. The specific method is as follows:

[0123] (1) Cell seeding: Keratinocytes were diluted with MEM medium to a seeding density (the degree of confluence reached 45%-60% 24 h after seeding), seeded into a 96-well plate with 200 μL of cell suspension per well, and placed in a CO2 incubator for 24 h±2 h.

[0124] (2) Dosing: Aspirate the culture medium in the 96-well plate and carry out the dosing operation. Dilute the sample 10 times and add it to the 96-well plate. Add normal cell culture medium to the blank / solvent control wells, 200 μL per well. After the dosing is completed, place the 96-well plate in a CO2 incubator and culture for 24 h ± 2 h.

[0125] (3) Test: After the sample is cultured for 24 h±2 h, all cells are collected, and then the cells are lysed by cell lysis or ultrasonic lysis, and then tested using an enzyme immunoassay kit. The test results are shown in Table 3 below.

[0126] Table 3. Results of the detection of the concentration of water channel proteins in each sample

[0127]

[0128]

[0129] It can be seen from Table 3 above that compared with Examples 2-13, the concentration of water channel proteins promoted by Comparative Examples 1-4 is not high. The specific reasons are as follows: Glycerol glucoside and trehalose derived from cyanobacteria are both polysaccharides, and the lack of any one of them will lead to a reduction in their total sugar content; and polysaccharides can promote the expression of keratinocyte water channel proteins, so the lack of any one of glycerol glucoside and trehalose derived from cyanobacteria will lead to a decrease in the efficacy of the composition in promoting the increase of water channel protein concentration. The total sugar content of Example 2-13 is high, so compared with Comparative Examples 1-4, the composition prepared therefrom significantly promotes the upregulation of water channel proteins, so Example 2-13 was selected for the next step of human efficacy testing.

[0130] Test Example 4: Human efficacy test - Determination of the efficacy of the stratum corneum in promoting the expression of aquaporin in skin cells

[0131] Samples were taken from the compositions prepared in Examples 2-13, and the samples were subjected to human efficacy tests, that is, the efficacy of the stratum corneum in promoting the expression of aquaporin in skin cells was determined. The specific method is as follows:

[0132] (1) 30 subjects aged between 18 and 45 years (excluding pregnant or lactating women). The baseline value of the capacitive skin moisture meter in the forearm test area is required to be between 15 and 40. The subjects should have no serious systemic diseases, no immune deficiency or autoimmune diseases, no active allergic diseases, no history of severe allergies to skin care cosmetics, no use of hormone drugs and immunosuppressants in the past month, and no participation in other clinical trials. No external medications should be used on the test area 2-3 days before. Before the test, the subjects need to agree to clean the inner forearms of both hands, wipe them clean with a paper towel, and wait for 30 minutes in a constant temperature and humidity chamber (test temperature is (21±1)℃, relative humidity is (50±10)%RH). Mark the measurement area on the inner forearms of both hands of the subjects.

[0133] (2) During the experiment, the inner sides of the left and right arms were marked with 3×3 cm 2 Test area, multiple areas are marked on the same arm at the same time. The test product (composition of Example 2-13) is prepared into a 2% aqueous solution and a blank control is randomly distributed on the left and right arms. The MMV value of the test area is tested and recorded. Each area is measured in parallel 3 times. First measure the blank value of each test area, and then press 2±0.1mg sample / cm 2 The test product was evenly applied to the test area. The skin moisture content of the test area and the blank control area was measured 4 hours after application. The test of the same subject was completed by the same staff, and the test results are shown in Table 4 below.

[0134] Table 4. Test results of stratum corneum water content in each sample

[0135] sample Water content of stratum corneum 0h (%) Water content of stratum corneum 4h (%) Example 2 22.03 27.67 Example 3 19.75 24.71 Example 4 21.23 24.53 Example 5 20.45 25.85 Example 6 19.69 32.18 Example 7 23.12 30.62 Example 8 22.42 33.27 Example 9 20.71 35.78 Example 10 20.68 38.57 Embodiment 11 20.67 45.37 Example 12 19.77 46.79 Example 13 23.14 42.34 Blank 22.31 23.48

[0136] It can be seen from Table 4 above that the compositions prepared in Examples 11-12 have better moisturizing effects than other examples. The moisturizing performance of Examples 11-12 of the present invention is better than that of other examples because both glycerol glucoside and trehalose derived from cyanobacteria are polysaccharides, and when the two are combined into a composition, they synergize to promote better moisturizing effects on the human body.

[0137] In summary, when the present invention uses glycerol glucoside and trehalose from cyanobacteria to prepare a composition, it has significant advantages in increasing the concentration of aquaporin and moisturizing; it has a very obvious promoting effect on the expression of aquaporin in skin cells, the skin barrier, and the deep moisturizing of the skin. The lack of any substance or the reduction in dosage will lead to a deterioration of the effect. The above-mentioned moisturizing composition that promotes the expression of aquaporin can be widely used in the preparation of cosmetics, for example, in moisturizing essences.

[0138] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A moisturizing composition for promoting the expression of aquaporin, characterized in that: The invention comprises glycerol glucoside and trehalose derived from cyanobacteria in a mass ratio of 1-10:1-5.

2. The moisturizing composition for promoting the expression of aquaporin according to claim 1, characterized in that: The cyanobacteria are Synechocystis.

3. The moisturizing composition for promoting the expression of aquaporin according to claim 2, characterized in that: The Synechocystis sp. is cultured in a culture medium containing a salt component.

4. The moisturizing composition for promoting the expression of aquaporin according to claim 3, characterized in that: The concentration of the salt component in the culture medium is 3-4%.

5. The moisturizing composition for promoting the expression of aquaporin according to claim 1, characterized in that: The preparation method of the cyanobacteria-derived glycerol glucoside comprises: (1) collecting and washing the algal mud obtained by culturing Synechocystis in a medium containing salt components; (2) reducing the water content of the Synechocystis algae mud to 50% solid content, adding 1-5 mL of mixed biological enzyme per liter, and performing enzymolysis at 55-65° C. for 5-8 hours to obtain a preliminary enzymolysis solution; (3) pouring the preliminary enzymatic hydrolysate into a high temperature and high pressure reactor, adding 2-3 times the volume of 1-2% hydrogen peroxide aqueous solution per liter of the preliminary enzymatic hydrolysate, oxidizing under high temperature and high pressure reaction conditions, the oxidation reaction time is 2-3 hours, and obtaining an oxidation product; (4) pressing and filtering the oxidation product to obtain a clear liquid rich in small molecular organic carbon source active substances; (5) using activated carbon adsorption, and then using 45%-60% ethanol concentration (v / v) eluent to separate and elute the glycerol glucoside to obtain a glycerol glucoside solution; (6) Using a nanofiltration membrane to filter and separate the glycerol glucoside solution to collect the glycerol glucoside product.

6. The moisturizing composition for promoting the expression of aquaporin according to claim 5, characterized in that: The mixed biological enzymes include cellulase, pectinase, glucose oxidase and acid protease in a mass ratio of 1-5:1-5:1-2:0.5-1; And / or, the high temperature and high pressure reaction conditions have a temperature of 200-300° C. and a pressure of 9-10 MPa.

7. Use of the moisturizing composition for promoting the expression of aquaporin according to any one of claims 1 to 6 in the preparation of cosmetics.

8. A moisturizing essence, characterized in that: It comprises the moisturizing composition for promoting the expression of aquaporin as claimed in any one of claims 1 to 6, wherein the mass percentage of the moisturizing composition for promoting the expression of aquaporin in the moisturizing essence is 1-3%.

9. The moisturizing essence according to claim 8, characterized in that: The ingredients of the moisturizing essence also include moisturizers, chelating agents, thickeners, preservatives, emollients, emulsifiers, fragrances and water; Preferably, based on the weight percentage of the moisturizing essence, the moisturizer is 1-3% glycerol; the chelating agent is 0.02-0.05% EDTA-2Na; the thickener is 0.5-1% ammonium acryloyldimethyltaurate / VP copolymer; the preservative is 0.2-0.5% phenoxyethanol; the emollient is 3-5% caprylic / capric triglyceride; the emulsifier is 0.4-0.6% polyglyceryl-10 stearate; the fragrance is 0.001-0.003% essence; the balance is water.

10. The moisturizing essence according to claim 9, characterized in that: The preparation method of the moisturizing essence comprises: (1) mixing the moisturizer, the chelating agent, the thickener, the emollient, the emulsifier and the water at a temperature of 80 to 85° C., stirring until completely dissolved, and sterilizing by heat preservation to obtain a phase A mixture, which is then kept warm; (2) stirring and heating the moisturizing composition for promoting the expression of aquaporin and the fragrance at 80-85° C. to completely dissolve to obtain a phase B mixture, and keeping the mixture warm; (3) adding the phase A mixture to the phase B mixture under homogenous stirring, homogenizing, stirring and keeping warm, and then starting to cool; (4) After the temperature drops to 60° C., add the preservative, stir evenly and serve.

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