Livestock and poultry traditional Chinese medicine extract feed additive capable of replacing antibiotics and preparation method of livestock and poultry traditional Chinese medicine extract feed additive

Abstract

The invention relates to the technical field of animal feed, in particular to a livestock and poultry traditional Chinese medicine extract feed additive capable of replacing antibiotics and a preparation method of the livestock and poultry traditional Chinese medicine extract feed additive. The traditional Chinese medicine extract composition and the livestock and poultry traditional Chinese medicine extract feed additive provided by the invention are composed of extracts of astragalus membranaceus, dandelion, rhizoma polygonati, agastache rugosus, scutellaria baicalensis, wild chrysanthemum, mangnolia officinalis, folium isatidis, hawthorn, fructus cannabis, honeysuckle, isatis root, artemisia apiacea, schisandra chinensis, Chinese pulsatilla root, gallnut and liquorice. The composition has the characteristics of no pollution, no residue, better safety, small toxic and side effects, no drug resistance and the like. The result of the specific embodiment of the invention shows that the traditional Chinese medicine extract composition and the livestock and poultry traditional Chinese medicine extract feed additive provided by the invention can improve the immune function of livestock and poultry, promote the growth and digestive absorption of livestock and poultry, and have the effects of diminishing inflammation, resisting bacteria, resisting diarrhea caused by pathogenic microorganisms, and regulating the intestinal flora structure and balancing.

Description

Technical Field

[0001] The invention relates to the technical field of animal feed, and in particular to a feed additive of livestock and poultry traditional Chinese medicine extract which can be used to replace antibiotics and a preparation method thereof. Background Art

[0002] In recent years, the use of antibiotics, chemical synthetic drugs and steroid hormones in feed, in addition to their anti-disease effects, has also promoted the effective utilization of nutrients in feed and promoted production. However, chemical drug feed additives have disadvantages to varying degrees, such as reducing the body's production performance, causing toxic side effects and drug residues, and endangering human health. In my country, how to ensure the safety of livestock and poultry products, and thus protect people's health and protect the ecology, has also become a major issue of common concern to the government and the whole society. On July 9, 2019, the Ministry of Agriculture and Rural Affairs issued Announcement No. 194, withdrawing all growth-promoting drug feed additives except traditional Chinese medicine from January 1, 2020. How to ensure that my country's feed additives become "green, efficient and safe" products is an urgent problem to be solved in the livestock and poultry farming industry.

[0003] Chinese herbal medicine feed additives are the hot spots of animal nutrition research in recent years. According to literature reports, there are more than 200 Chinese herbal medicines currently used for feed additive raw materials, and some invention patents also disclose the formulas of various Chinese herbal medicine feed additives, and the effects and costs of various formulas are also different, and the preparation methods of Chinese herbal medicines are also ever-changing. For example, CN103005210 B discloses a pig feed additive that can replace antibiotics, which is composed of Shenqu, Angelica, Astragalus, Hawthorn, Codonopsis, citric acid, lactic acid, coated phosphoric acid, microecological preparations, yeast cell wall polysaccharides, composite plant essential oils and carriers. For another example, CN103947907B discloses another pig immune enhancement feed additive that can replace antibiotics, which is composed of lotus leaf polysaccharides, Choerospondias fasciata extract, Angelica powder, Perilla leaf powder, Evergreen powder, Lianqiancao powder, microecological preparations, allicin, multivitamins, composite minerals and carriers. The formulas in the above patents are mostly Chinese medicines for nourishing yin, tonifying the kidney and nourishing the stomach, or Chinese medicines for enhancing non-specific immune function with polysaccharides, and their effects and application range are greatly limited. Therefore, developing a "green, efficient and safe" feed additive product can effectively serve the local area and solve the "bottleneck" problem. Summary of the invention

[0004] The purpose of the present invention is to provide a feed additive of Chinese herbal medicine extracts for livestock and poultry that can be used to replace antibiotics and a preparation method thereof, so as to solve the problems existing in the above-mentioned prior art. The present invention uses Chinese herbal medicine extracts as feed additives to improve feed conversion rate, body immunity, disease resistance, anti-stress ability, antibacterial and antiviral effects, while also improving the quality of livestock and poultry products, thereby generating good economic and social benefits.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] The present invention provides a combination of Chinese herbal extracts that can be used to replace antibiotics. The combination of Chinese herbal extracts comprises the following components in parts by weight:

[0007] 25-36 parts of Astragalus extract, 15-22 parts of Dandelion extract, 15-26 parts of Polygonatum sibiricum extract, 15-20 parts of Patchouli extract, 12-18 parts of Scutellaria baicalensis extract, 10-18 parts of Chrysanthemum indica extract, 12-19 parts of Magnolia officinalis extract, 10-18 parts of Isatis indigotica extract, 12-18 parts of Crataegus pinnatifida extract, 7-16 parts of Cannabis sativa seed extract, 5-15 parts of Honeysuckle extract, 5-12 parts of Isatis indigotica extract, 6-15 parts of Artemisia annua extract, 6-14 parts of Schisandra chinensis extract, 7-12 parts of Pulsatilla striata extract, 5-10 parts of Galla chinensis extract and 12-17 parts of Licorice extract.

[0008] Preferably, the Chinese herbal extract combination comprises the following components in parts by weight:

[0009] 25 parts of Astragalus extract, 15 parts of Dandelion extract, 17 parts of Polygonatum sibiricum extract, 15 parts of Patchouli extract, 16 parts of Scutellaria baicalensis extract, 16 parts of Chrysanthemum indica extract, 12 parts of Magnolia officinalis extract, 10 parts of Isatis indigotica extract, 17 parts of Crataegus pinnatifida extract, 7 parts of Cannabis sativa seed extract, 8 parts of Honeysuckle extract, 5 parts of Isatis indigotica extract, 6 parts of Artemisia annua extract, 6 parts of Schisandra chinensis extract, 7 parts of Pulsatilla striata extract, 5 parts of Galla chinensis extract and 17 parts of Licorice extract.

[0010] Preferably, the preparation method of the astragalus extract comprises the steps of crushing, water extraction, alcohol extraction, centrifugation and drying the astragalus in sequence to obtain the astragalus extract;

[0011] The preparation method of the dandelion extract comprises the steps of sequentially crushing, water extraction, ultrasonication, filtration and freeze drying the dandelion to obtain the dandelion extract;

[0012] The preparation method of the polygonatum sibiricum extract comprises the steps of sequentially crushing polygonatum sibiricum, ultrasonic alcohol extraction, filtering and vacuum drying to obtain the polygonatum sibiricum extract;

[0013] The preparation method of the Patchouli extract comprises the steps of crushing Patchouli in sequence, extracting with petroleum ether and concentrating the Patchouli to obtain the Patchouli extract;

[0014] The preparation method of the scutellaria baicalensis extract comprises the steps of sequentially crushing, alcohol extraction, filtering and freeze-drying the scutellaria baicalensis to obtain the scutellaria baicalensis extract;

[0015] The method for preparing the wild chrysanthemum extract comprises the steps of sequentially crushing the wild chrysanthemum, extracting with alcohol, filtering and vacuum drying to obtain the wild chrysanthemum extract;

[0016] The preparation method of the Magnolia bark extract comprises the steps of sequentially crushing the Magnolia bark, performing microwave alcohol extraction, filtering and vacuum drying to obtain the Magnolia bark extract;

[0017] The preparation method of the isatis indigo leaf extract comprises the steps of sequentially crushing the isatis indigo leaf, performing microwave alcohol extraction, filtering and vacuum drying to obtain the isatis indigo leaf extract;

[0018] The preparation method of the hawthorn extract comprises the steps of sequentially crushing hawthorn, enzymolysis, ultrasonic alcohol extraction, filtration and vacuum drying to obtain the hawthorn extract;

[0019] The preparation method of the hemp seed extract comprises the steps of crushing the hemp seed in sequence, extracting with petroleum ether, filtering and freeze-drying to obtain the hemp seed extract;

[0020] The preparation method of the honeysuckle extract comprises the steps of sequentially crushing honeysuckle, alcohol extraction, filtering and vacuum drying to obtain the honeysuckle extract;

[0021] The preparation method of the Radix Isatidis extract comprises the steps of sequentially crushing the Radix Isatidis, extracting with alcohol, filtering and vacuum drying the Radix Isatidis to obtain the Radix Isatidis extract;

[0022] The preparation method of the artemisia annua extract comprises the steps of crushing the artemisia annua in sequence, extracting with petroleum ether, washing with a NaOH solution, filtering, concentrating and vacuum drying the artemisia annua extract;

[0023] The preparation method of the Schisandra chinensis extract comprises the steps of sequentially crushing, alcohol extracting, filtering and vacuum drying the Schisandra chinensis to obtain the Schisandra chinensis extract;

[0024] The preparation method of the Pulsatilla chinensis extract comprises the steps of sequentially crushing, water extracting, filtering and vacuum concentrating the Pulsatilla chinensis to obtain the Pulsatilla chinensis;

[0025] The preparation method of the gallnut extract comprises the steps of sequentially crushing the gallnut, ultrasonically extracting with alcohol, filtering and vacuum drying the gallnut to obtain the gallnut extract;

[0026] The preparation method of the licorice extract comprises the steps of sequentially crushing licorice, ultrasonic alcohol extraction, suction filtration, filtration and vacuum drying to obtain the licorice extract.

[0027] Preferably, when preparing the astragalus extract, the solid-liquid ratio of the water extraction is 1g: (8-10)mL, the temperature is 100°C, and the time is 3h; the solvent used in the alcohol extraction is 90% ethanol, the temperature is 4°C, and the time is 12h; the speed of the centrifugation is 8000-10000r / min, and the time is 10min;

[0028] When preparing the dandelion extract, the water extraction material-liquid ratio is 1g: (20-25)mL, and the time is 20-24h; the power of the ultrasound is 120W, the temperature is 60°C, and the time is 1.5-2h;

[0029] When preparing the polygonatum sibiricum extract, the solvent used in the ultrasonic alcohol extraction is 75% ethanol; the material-liquid ratio of the ultrasonic alcohol extraction is 1g: (40-45)mL, the temperature is 37°C, the power is 300W, the number of ultrasonic times is 3-4 times, and the time of each ultrasonic time is 30min;

[0030] When preparing the Patchouli extract, the material-liquid ratio of the petroleum ether extraction is 1 g: (5-8) mL, the temperature is 50° C., the number of times is 3-4 times, and the time of each petroleum ether extraction is 12-24 hours;

[0031] When preparing the scutellaria baicalensis extract, the solvent used in the alcohol extraction is 75% ethanol; the solid-liquid ratio of the alcohol extraction is 1g: (8-10)mL, the temperature is 80°C, the number of times is 2, and the time of each alcohol extraction is 1.5-2h;

[0032] When preparing the wild chrysanthemum extract, the solvent used in the alcohol extraction is 75% ethanol; the solid-liquid ratio of the alcohol extraction is 1g: (18-20)mL, the temperature is 60°C, and the time is 1.5-2h;

[0033] When preparing the Magnolia officinalis extract, the solvent used in the microwave alcohol extraction is 75% ethanol; the material-liquid ratio of the microwave alcohol extraction is 1g: (18-20)mL, the temperature is 70°C, the power is 240W, and the time is 5-7min;

[0034] When preparing the isatis indigofera extract, the solvent used in the microwave alcohol extraction is 80% ethanol; the material-liquid ratio of the microwave extraction is 1g: (20-25)mL, the temperature is 60°C, the power is 1000W, and the time is 3-5min;

[0035] When preparing the hawthorn extract, the enzymes used in the enzymatic hydrolysis are cellulase and pectinase; the amount of the cellulase used is 4% to 5% of the mass of the hawthorn obtained after crushing; the amount of the pectinase used is 5% to 6% of the mass of the hawthorn obtained after crushing; the temperature of the enzymatic hydrolysis is 55°C, the pH is 5.5 to 6.5, and the time is 1.5 to 2h; the solvent used in the ultrasonic alcohol extraction is 75% ethanol; the material-liquid ratio of the ultrasonic alcohol extraction is 1g: (30 to 35)mL, the temperature is 40°C, and the time is 30min;

[0036] When preparing the hemp seed extract, the material-liquid ratio of the petroleum ether extraction is 1g: (8-12)mL, the temperature is 60°C, and the time is 6h;

[0037] When preparing the honeysuckle extract, the solvent used in the alcohol extraction is 80% ethanol; the solid-liquid ratio of the alcohol extraction is 1g: (8-10)mL, the temperature is 80°C, the number of times is 2, and the time of each alcohol extraction is 1.5-2h;

[0038] When preparing the Radix Isatidis extract, the solvent used in the alcohol extraction is 75% ethanol; the solid-liquid ratio of the alcohol extraction is 1g: (8-10)mL, the temperature is 90°C, and the time is 30min;

[0039] When preparing the Artemisia annua extract, the material-liquid ratio of the petroleum ether extraction is 1 g: (18-20) mL, the temperature is 50° C., and the time is 2-2.5 h;

[0040] When preparing the Schisandra chinensis extract, the solvent used in the alcohol extraction is 80% ethanol; the solid-liquid ratio of the alcohol extraction is 1g: (6-8)mL, the temperature is 80°C, and the time is 30-40min;

[0041] When preparing the Pulsatilla chinensis extract, the water extraction solid-liquid ratio is 1 g: (10-15) mL, the temperature is 80° C., the number of times is 3, and the time of each water extraction is 5 hours;

[0042] When preparing the gallnut extract, the solvent used in the ultrasonic alcohol extraction is a mixed solution of 75% ethanol and 1% acetic acid; the volume of 75% ethanol and 1% acetic acid in the mixed solution is 65:1; the solid-liquid ratio of the alcohol extraction is 1g: (20-25)mL, the temperature is 50°C, and the time is 1-1.5h;

[0043] When preparing the licorice extract, the solvent used in the ultrasonic extraction is 60% ethanol; the solid-liquid ratio of the alcohol extraction is 1g:15mL, the temperature is 80°C, and the time is 30min.

[0044] The present invention provides the use of the above-mentioned Chinese medicine extract combination in the preparation of a feed additive that can be used to replace antibiotics.

[0045] The invention provides a feed additive which can be used to replace antibiotics. The feed additive comprises the above-mentioned traditional Chinese medicine extract combination.

[0046] The present invention provides a method for preparing the above-mentioned feed additive, which comprises the following steps:

[0047] The astragalus extract, the dandelion extract, the polygonatum extract, the patches extract, the scutellaria extract, the wild chrysanthemum extract, the magnolia bark extract, the isatis indigotica extract, the hawthorn extract, the hemp seed extract, the honeysuckle extract, the isatis root extract, the artemisia annua extract, the schisandra chinensis extract, the pulsatilla extract, the gallnut extract and the liquorice extract are mixed evenly to obtain the feed additive.

[0048] The present invention provides the use of the above-mentioned Chinese medicine extract combination or the above-mentioned feed additive in preparing feed that can be used to replace antibiotics.

[0049] The invention provides a feed which can be used to replace antibiotics. The feed comprises the feed additive and a basic diet.

[0050] The present invention provides the use of the above-mentioned Chinese medicine extract combination, the above-mentioned feed additive or the above-mentioned feed in any of the following items:

[0051] (1) Application in improving the immunity of livestock and poultry;

[0052] (2) Application in the preparation of products for improving the immunity of livestock and poultry;

[0053] (3) Application in promoting the growth of livestock and poultry;

[0054] (4) Application in the preparation of products that promote the growth of livestock and poultry;

[0055] (5) Application in improving the survival rate of livestock and poultry;

[0056] (6) Application in the preparation of products for improving the survival rate of livestock and poultry;

[0057] (7) Application in improving the jejunal mucosal tissue structure of livestock and poultry;

[0058] (8) Application in the preparation of products for improving the jejunal mucosal tissue structure of livestock and poultry;

[0059] (9) Application in improving the intestinal flora structure of livestock and poultry;

[0060] (10) Application in the preparation of products for improving the intestinal flora structure of livestock and poultry.

[0061] Further preferably, the livestock and poultry are pigs and the poultry are chickens.

[0062] The present invention discloses the following technical effects:

[0063] The invention provides a Chinese medicine extract combination and a Chinese medicine extract feed additive for livestock and poultry that can be used to replace antibiotics. The Chinese medicine extract combination and the Chinese medicine extract feed additive for livestock and poultry are composed of extracts of astragalus, dandelion, polygonatum, patchouli, scutellaria, wild chrysanthemum, magnolia bark, isatis indigotica, hawthorn, hemp seed, honeysuckle, isatis root, artemisia annua, schisandra chinensis, pulsatilla, gallnut and liquorice, and have the characteristics of no pollution, no residue, good safety, little toxic and side effects, no drug resistance, etc. The Chinese medicine extract combination and the Chinese medicine extract feed additive for livestock and poultry can improve the immune function of livestock and poultry, promote the growth and digestion and absorption of livestock and poultry, and have the effects of anti-inflammatory, antibacterial, resistance to diarrhea caused by pathogenic microorganisms, and regulating the structure and balance of intestinal flora. The Chinese medicine extract combination of the present invention is based on the theory of traditional Chinese medicine and traditional prescriptions, with four herbs, namely, astragalus, dandelion, polygonatum, and patchouli as the main herbs; five herbs, namely, scutellaria, wild chrysanthemum, magnolia bark, isatis indigotica, and hawthorn as the subordinate herbs; seven herbs, namely, hemp seed, honeysuckle, isatis root, artemisia annua, schisandra chinensis, pulsatilla scabra, and gallnut as the adjuvant herbs; and one herb, namely, licorice as the envoy. Through the compatibility of the drugs and the test of clinical efficacy, the compatibility ratio of the extracts of astragalus, dandelion, polygonatum, patchouli, scutellaria, wild chrysanthemum, magnolia bark, isatis indigotica, hawthorn, hemp seed, honeysuckle, isatis root, artemisia annua, schisandra chinensis, pulsatilla scabra, gallnut and licorice is determined, and a Chinese medicine extract feed additive for livestock and poultry that can be used to replace antibiotics is prepared, and its advantages are as follows:

[0064] 1. It can improve the immune function of livestock and poultry, and promote the growth and digestion and absorption of livestock and poultry. The extracts of hawthorn and hemp seed in the Chinese medicine extract combination contain rich physiologically active substances such as protein, polysaccharides, glycosides, organic acids, carotene, amylase, pepsin, lipase, vitamin A, vitamin B and vitamin C, which can promote immune enhancement or immune stimulation, activate innate immune cells such as macrophages and natural killer cells, enhance the phagocytic function of macrophages, promote the proliferation of T lymphocytes and B lymphocytes, promote the generation of antibodies, and promote the secretion of cytokines and other functions; it can promote the gastrointestinal motility and digestive gland secretion of livestock and poultry, improve the digestibility and utilization rate of nutrients, enhance metabolism, promote the growth of livestock and poultry, and reduce the feed-to-meat ratio; it can enhance the disease prevention and disease resistance of livestock and poultry, improve the defense ability of livestock and poultry and alleviate stress response, effectively improve the intestinal environment of livestock and poultry, enhance the body's absorption and utilization of nutrients, and improve the digestion and utilization rate of plant protein in feed by livestock and poultry. In addition, the Chinese medicine extract combination provided by the present invention has the effects of strengthening the body and eliminating evil, and replenishing the middle and replenishing qi. After being fed to livestock and poultry, it can increase the antibody level of Newcastle disease and classical swine fever; increase the average daily weight gain of livestock and poultry, reduce the feed-to-meat ratio, and produce good economic benefits.

[0065] 2. It has anti-inflammatory, antibacterial and anti-diarrhea effects caused by pathogenic microorganisms. The Chinese herbal extracts such as dandelion, scutellaria, polygonatum, isatis root, and schisandra contain rich polysaccharides, organic acids, alkaloids, glycosides, and volatile oils. Among them, polysaccharides can improve the barrier function of the intestinal mucosa to reduce the release of inflammatory factors, inhibit inflammatory hyperplasia, and the secretion of inflammatory cytokines (IL-6, CRP, IL-1β, TNF-α, and NF-κB). Organic acids can inhibit the production of inflammatory mediators, thereby reducing inflammatory reactions; they can regulate the function of immune cells to inhibit excessive immune reactions, thereby reducing inflammation; they can regulate the acid-base balance in the body, thereby reducing inflammatory reactions. Glycosides can inhibit the generation of inflammatory mediators such as prostaglandin E2 and nitric oxide; can increase the activity of antioxidant enzymes such as superoxide dismutase, thereby enhancing the body's ability to scavenge free radicals; can reduce the levels of proinflammatory cytokines such as tumor necrosis factor α and interleukin-1β, while increasing the levels of anti-inflammatory cytokines such as interferon γ and interleukin-4; can inhibit the activation of nuclear factor κB, and participate in regulating the expression of multiple inflammatory genes. At the same time, the feed additive provided by the present invention has the effects of clearing away heat and detoxification, replenishing the middle and replenishing qi, anti-inflammatory and antibacterial. The results of the specific embodiments of the present invention show that after livestock and poultry are fed with the feed additive provided by the present invention, the content of IFN-γ in serum can be increased, the content of IL-1β, IL-6 and TNF-α in serum can be reduced, the survival rate of livestock and poultry can be improved, the abnormalities of feces and respiratory tract can be reduced, the villus height and crypt depth of the jejunal mucosal tissue structure of livestock and poultry can be increased, the number of Escherichia coli in the ileum and rectum can be reduced, the number of bifidobacteria and lactobacilli can be increased, and the intestinal microecology of livestock and poultry can be kept balanced.

[0066] 3. Regulate the structure and balance of intestinal flora. The Chinese herbal extract combination of astragalus, hemp seed, honeysuckle, wild chrysanthemum, schisandra chinensis, etc. contains rich protein, volatile oil, flavonoids, polysaccharides, saponins and other physiologically active substances, which can regulate intestinal flora and promote digestive juice secretion. Intestinal microorganisms can degrade polysaccharides into short-chain fatty acids, mainly acetic acid, propionic acid and butyric acid. Metabolism produces a large amount of acid, which leads to a decrease in pH. Low pH inhibits the growth of some intestinal pathogens and can also change the metabolic absorption of colon cells. After flavonoids can be decomposed into small molecules by intestinal microorganisms, their metabolites can inhibit the growth of harmful intestinal microorganisms, promote the proliferation of beneficial microorganisms, maintain the balance of intestinal flora, and benefit the health of the intestinal microenvironment. Saponins can promote the growth of intestinal microorganisms, adjust the phenomenon of intestinal flora imbalance, increase the number of bifidobacteria and lactobacilli, decrease the number of enterococci and enterobacteria, and restore the ratio of positive bacteria to negative bacteria to normal. DETAILED DESCRIPTION

[0067] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0068] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0069] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0070] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to the skilled artisan. The present invention description and examples are exemplary only.

[0071] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0072] The ethanol described in the present invention is all content by volume percentage.

[0073] Example 1

[0074] A feed additive that can be used to replace antibiotics (a feed additive for livestock and poultry made from Chinese herbal medicine extracts that can replace antibiotics), the components of which are as follows, measured by weight:

[0075] 25 parts of Astragalus extract, 15 parts of Dandelion extract, 17 parts of Polygonatum sibiricum extract, 15 parts of Patchouli extract, 16 parts of Scutellaria baicalensis extract, 16 parts of Chrysanthemum indica extract, 12 parts of Magnolia officinalis extract, 10 parts of Isatis indigotica extract, 17 parts of Crataegus pinnatifida extract, 7 parts of Cannabis sativa seed extract, 8 parts of Honeysuckle extract, 5 parts of Isatis indigotica extract, 6 parts of Artemisia annua extract, 6 parts of Schisandra chinensis extract, 7 parts of Pulsatilla striata extract, 5 parts of Galla chinensis extract and 17 parts of Licorice extract, i.e. : Astragalus extract: Dandelion extract: Polygonatum sibiricum extract: Patchouli extract: Scutellaria baicalensis extract: Chrysanthemum officinale extract: Magnolia officinalis extract: Isatis indigotica extract: Crataegus pinnatifida extract: Hemp seed extract: Honeysuckle extract: Isatis indigotica root extract: Artemisia annua extract: Schisandra chinensis extract: Pulsatilla striata extract: Gallnut chinensis extract: Licorice extract = 25:15:17:15:16:16:12:10:17:7:8:5:6:6:7:5:17.

[0076] The preparation method of the feed additive comprises the following specific steps:

[0077] Astragalus extract: After the astragalus is dried, it is crushed and passed through a 60-mesh sieve. Distilled water is added in a ratio of 1g:9mL. The mixture is boiled at 100°C for 3h and then filtered. The filtrate is concentrated to 45% of the original volume. 3.5 times the volume of the concentrated solution of 90% ethanol is added and mixed evenly. The mixture is extracted at 4°C for 12h. After that, the precipitate is centrifuged at 9000r / min for 10min to obtain the precipitate. The precipitate is vacuum dried at 65°C for 7-9h and crushed and passed through a 60-mesh sieve to obtain the astragalus extract.

[0078] Dandelion extract: Dry the dandelion, crush it through a 40-mesh sieve, add distilled water at a solid-liquid ratio of 1 g:23 mL, soak at room temperature for 22 hours, then ultrasonicate at 120 W and 60 ° C for 1.75 hours, filter, freeze-dry the filtrate at -55 to -60 ° C for 24 to 30 hours, then crush it through a 60-mesh sieve to obtain the dandelion extract.

[0079] Polygonatum sibiricum extract: Dry and crush the polygonatum sibiricum, pass through a 40-mesh sieve, add 75% ethanol in a ratio of 1g:43mL, ultrasonicate at 300W and 37℃ for 30min, ultrasonicate 3 times, filter, vacuum dry the filtrate at 60℃ for 8-10h, crush and pass through a 60-mesh sieve to obtain the polygonatum sibiricum extract.

[0080] Patchouli extract: Dry and crush the Patchouli, pass through a 40-mesh sieve, add petroleum ether in a solid-liquid ratio of 1 g: 7 mL, extract at 50°C for 4 times, each time for 18 hours, concentrate on a rotary evaporator, dry naturally, crush and pass through a 60-mesh sieve to obtain the Patchouli extract.

[0081] Scutellaria baicalensis extract: Dry and crush the scutellaria baicalensis, pass through a 40-mesh sieve, add 75% ethanol in a solid-liquid ratio of 1g:9mL, extract at 80°C under reflux for 2 times, each time for 1.75h, filter, freeze-dry the filtrate at -55 to -60°C for 24 to 30h, crush and pass through a 60-mesh sieve to obtain the scutellaria baicalensis extract.

[0082] Wild chrysanthemum extract: dry and crush the wild chrysanthemum, pass through a 40-mesh sieve, add 75% ethanol in a solid-liquid ratio of 1g:19mL, extract at 60°C for 1.75h, filter, and dry the filtrate in a vacuum at 60°C for 8-10h, crush and pass through a 60-mesh sieve to obtain the wild chrysanthemum extract.

[0083] Magnolia bark extract: Dry and crush the Magnolia bark, pass through a 40-mesh sieve, add 75% ethanol in a solid-liquid ratio of 1 g:19 mL, microwave at 70°C and 240 W for 6 min, filter, vacuum dry the filtrate at 60°C for 8 to 10 h, crush and pass through a 60-mesh sieve to obtain the Magnolia bark extract.

[0084] Folium Isatidis Extract: Dry and crush the Folium Isatidis, pass through a 40-mesh sieve, add 80% methanol in a solid-liquid ratio of 1g:23mL, microwave at 60°C, 1000W for 4min, filter the filtrate and dry it in a vacuum at 60°C for 8-10h, crush and pass through a 60-mesh sieve to obtain the Folium Isatidis Extract.

[0085] Hawthorn extract: The hawthorn is dried and crushed, passed through a 40-mesh sieve, 4.5% of the mass of the crushed hawthorn by cellulase and 5.5% of the mass of the crushed hawthorn by pectinase are added, and enzymolysis is carried out at 55°C and pH 6 for 1.75 hours to obtain enzymolysis hawthorn powder; then, 75% ethanol is added at a solid-liquid ratio of 1g:33mL, ultrasonication is carried out at 40°C for 30 minutes, and filtration is carried out. The filtrate is vacuum dried at 60°C for 8-10 hours, crushed and passed through a 60-mesh sieve to obtain the hawthorn extract.

[0086] Hemp seed extract: Dry and crush the hemp seed, pass through a 60-mesh sieve, add petroleum ether at a solid-liquid ratio of 1g:10mL, condense and reflux at 60°C for 6h, let stand for 30min and then filter, extract the residue again once, dry the obtained extract at 60°C in vacuum for 8-10h, crush and pass through a 60-mesh sieve to obtain the hemp seed extract.

[0087] Honeysuckle extract: Dry and crush the honeysuckle, pass through a 40-mesh sieve, add 80% ethanol in a solid-liquid ratio of 1g:9mL, extract under reflux at 80°C twice, each time for 1.75h, filter the filtrate and vacuum dry at 60°C for 8-10h to obtain the honeysuckle extract.

[0088] Radix Isatidis Extract: Dry and crush the Radix Isatidis, pass through a 40-mesh sieve, add 75% ethanol in a solid-liquid ratio of 1 g:9 mL, extract at 90°C for 30 min, filter, freeze-dry the filtrate at -55 to -60°C for 24 to 30 h, crush and pass through a 60-mesh sieve to obtain the Radix Isatidis Extract.

[0089] Artemisia annua extract: Dry and crush the Artemisia annua leaves, pass through a 40-mesh sieve, add petroleum ether in a solid-liquid ratio of 1 g:19 mL, extract at 50°C for 2.25 h, filter at room temperature, concentrate under reduced pressure, add 2 wt.% NaOH solution for washing and filtration, wash 4 times with distilled water, concentrate on a rotary evaporator, and vacuum dry the resulting concentrate at 60°C for 8 to 10 h, crush and pass through a 60-mesh sieve to obtain the Artemisia annua extract.

[0090] Schisandra extract: Dry and crush the schisandra, pass through a 40-mesh sieve, add 80% ethanol in a ratio of 1 g:7 mL, extract at 80°C for 35 min, filter the filtrate and dry it in a vacuum at 60°C for 8 to 10 h, crush and pass through a 60-mesh sieve to obtain the schisandra extract.

[0091] Pulsatilla extract: Crush the Pulsatilla, pass through a 60-mesh sieve, add distilled water in a solid-liquid ratio of 1g:12mL, extract for 3 times, each extraction time is 5h, and the extraction temperature is 80℃; combine the filtrate, vacuum dry the filtrate at 60℃ for 8-10h, crush and pass through a 60-mesh sieve to obtain the Pulsatilla extract.

[0092] Gallnut extract: Crush the gallnut, pass through a 40-mesh sieve, add a mixture of 75% ethanol and 1.0wt.% hydrochloric acid in a solid-liquid ratio of 1g:22mL (the volume ratio of 75% ethanol and 1.0wt.% hydrochloric acid in the mixture is 65:1), perform ultrasonic extraction at 50°C for 1.25h, filter the filtrate and vacuum dry it at 60°C for 8-10h, crush and pass through a 60-mesh sieve to obtain the gallnut extract.

[0093] Licorice extract: Grind the licorice, pass through a 90-mesh sieve, add 60% ethanol in a solid-liquid ratio of 1 g:15 mL, ultrasonically extract at 80°C for 30 min, filter, and dry the filtrate in a vacuum at 60°C for 8 to 10 h, grind and pass through a 60-mesh sieve to obtain the licorice extract.

[0094] The raw materials prepared above are mixed uniformly according to the following mass proportions to obtain a traditional Chinese medicine feed additive that can replace antibiotics.

[0095] Example 2

[0096] A feed additive that can be used to replace antibiotics (a feed additive for livestock and poultry made from Chinese herbal medicine extracts that can replace antibiotics), the components of which are as follows, measured by weight:

[0097] 30 parts of Astragalus extract, 20 parts of Dandelion extract, 26 parts of Polygonatum sibiricum extract, 18 parts of Patchouli extract, 12 parts of Scutellaria baicalensis extract, 10 parts of Chrysanthemum indica extract, 19 parts of Magnolia officinalis extract, 18 parts of Isatis indigotica extract, 12 parts of Crataegus pinnatifida extract, 12 parts of Cannabis sativa seed extract, 5 parts of Honeysuckle extract, 8 parts of Isatis indigotica extract, 10 parts of Artemisia annua extract, 10 parts of Schisandra chinensis extract, 12 parts of Pulsatilla striata extract, 7 parts of Galla chinensis extract and 12 parts of Licorice extract.

[0098] The preparation method of the feed additive comprises the following specific steps:

[0099] Astragalus extract: After the astragalus is dried, it is crushed and passed through a 60-mesh sieve. Distilled water is added at a solid-liquid ratio of 1g:8mL, and the mixture is boiled at 100℃ for 3h and filtered. The filtrate is concentrated to 40% of the original volume, and 90% ethanol (3 times the volume of the concentrate) is added and mixed evenly. Extraction is performed at 4℃ for 12h, and then centrifuged at 8000r / min for 10min to obtain the precipitate. The precipitate is vacuum dried at 60℃ for 8-10h, and crushed and passed through a 60-mesh sieve to obtain the astragalus extract.

[0100] Dandelion extract: Dry the dandelion, crush it through a 40-mesh sieve, add distilled water at a solid-liquid ratio of 1 g:20 mL, soak at room temperature for 20 hours, ultrasonically treat at 120 W and 60°C for 1.5 hours, filter, freeze-dry the filtrate at -55 to -60°C for 24 to 30 hours, and then crush it through a 60-mesh sieve to obtain the dandelion extract.

[0101] Polygonatum sibiricum extract: Dry and crush the polygonatum sibiricum, pass through a 40-mesh sieve, add 75% ethanol in a ratio of 1g:40mL, ultrasonicate at 300W and 37℃ for 30min, ultrasonicate 3 times, filter the filtrate and vacuum dry at 60℃ for 8-10h, crush and pass through a 60-mesh sieve to obtain the polygonatum sibiricum extract.

[0102] Patchouli extract: Dry and crush the Patchouli, pass through a 40-mesh sieve, add petroleum ether in a solid-liquid ratio of 1g:5mL, extract at 50°C for 3 times, each time for 12h, concentrate on a rotary evaporator, dry naturally, crush and pass through a 60-mesh sieve to obtain the Patchouli extract.

[0103] Scutellaria baicalensis extract: Dry and crush the scutellaria baicalensis, pass through a 40-mesh sieve, add 75% ethanol in a solid-liquid ratio of 1g:8mL, extract at 80°C under reflux for 2 times, each time for 1.5h, filter, freeze-dry the filtrate at -55 to -60°C for 24 to 30h, crush and pass through a 60-mesh sieve to obtain the scutellaria baicalensis extract.

[0104] Wild chrysanthemum extract: dry and crush the wild chrysanthemum, pass through a 40-mesh sieve, add 75% ethanol in a solid-liquid ratio of 1g:18mL, extract at 60°C for 1.5h, filter, and vacuum dry the filtrate at 60°C for 8-10h, crush and pass through a 60-mesh sieve to obtain the wild chrysanthemum extract.

[0105] Magnolia bark extract: Dry and crush the Magnolia bark, pass through a 40-mesh sieve, add 75% ethanol in a solid-liquid ratio of 1g:18mL, microwave at 70℃ and 240W for 5min, filter the filtrate and vacuum dry at 60℃ for 8-10h, crush and pass through a 60-mesh sieve to obtain the Magnolia bark extract.

[0106] Folium Isatidis Extract: Dry and crush the Folium Isatidis, pass through a 40-mesh sieve, add 80% methanol at a solid-liquid ratio of 1 g:20 mL, microwave at 60°C, 1000 W for 3 min, filter, vacuum dry the filtrate at 60°C for 8 to 10 h, crush and pass through a 60-mesh sieve to obtain the Folium Isatidis Extract.

[0107] Hawthorn extract: The hawthorn is dried and crushed, passed through a 40-mesh sieve, 4% of the mass of the crushed hawthorn by cellulase and 5% of the mass of the crushed hawthorn by pectinase are added, and enzymolysis is carried out at 55°C and pH 5.5 for 1.5 hours to obtain enzymolysis hawthorn powder; then, 75% ethanol is added at a solid-liquid ratio of 1g:30mL, ultrasonication is carried out at 40°C for 30 minutes, and filtration is carried out. The filtrate is vacuum dried at 60°C for 8-10 hours, crushed and passed through a 60-mesh sieve to obtain the hawthorn extract.

[0108] Hemp seed extract: Dry and crush the hemp seed, pass through a 60-mesh sieve, add petroleum ether at a solid-liquid ratio of 1g:8mL, condense and reflux at 60°C for 6h, let stand for 30min and then filter, extract the residue again once, dry the obtained extract at 60°C in vacuum for 8-10h, crush and pass through a 60-mesh sieve to obtain the hemp seed extract.

[0109] Honeysuckle extract: Dry and crush the honeysuckle, pass through a 40-mesh sieve, add 80% ethanol at a solid-liquid ratio of 1 g:8 mL, extract under reflux at 80°C twice, each time for 1.5 h, filter, and vacuum dry the filtrate at 60°C for 8 to 10 h to obtain the honeysuckle extract.

[0110] Radix Isatidis Extract: Dry and crush the Radix Isatidis, pass through a 40-mesh sieve, add 75% ethanol in a solid-liquid ratio of 1 g:8 mL, extract at 90°C for 30 min, filter, freeze-dry the filtrate at -55 to -60°C for 24 to 30 h, crush and pass through a 60-mesh sieve to obtain the Radix Isatidis Extract.

[0111] Artemisia annua extract: Dry and crush the Artemisia annua leaves, pass through a 40-mesh sieve, add petroleum ether in a solid-liquid ratio of 1g:18mL, extract at 50°C for 2h, filter at room temperature, concentrate under reduced pressure, add 2wt.% NaOH solution for washing and filtration, wash 3 times with distilled water, concentrate on a rotary evaporator, and vacuum dry the obtained concentrate at 60°C for 8-10h, crush and pass through a 60-mesh sieve to obtain the Artemisia annua extract.

[0112] Schisandra extract: Dry and crush the schisandra, pass through a 40-mesh sieve, add 80% ethanol in a solid-liquid ratio of 1 g:6 mL, extract at 80°C for 30 min, filter, and dry the filtrate in a vacuum at 60°C for 8 to 10 h, crush and pass through a 60-mesh sieve to obtain the schisandra extract.

[0113] Pulsatilla extract: Crush the Pulsatilla, pass through a 60-mesh sieve, add distilled water in a solid-liquid ratio of 1g:10mL, extract for 3 times, each extraction time is 5h, and the extraction temperature is 80℃; combine the filtrate, vacuum dry the filtrate at 60℃ for 8-10h, crush and pass through a 60-mesh sieve to obtain the Pulsatilla extract.

[0114] Gallnut extract: Crush the gallnut, pass through a 40-mesh sieve, add a mixture of 75% ethanol and 1.0wt.% hydrochloric acid in a solid-liquid ratio of 1g:20mL (the volume ratio of 75% ethanol and 1.0wt.% hydrochloric acid in the mixture is 65:1), ultrasonically extract at 50°C for 1h, filter, vacuum dry the filtrate at 60°C for 8-10h, crush and pass through a 60-mesh sieve to obtain the gallnut extract.

[0115] Licorice extract: Grind the licorice, pass through a 90-mesh sieve, add 60% ethanol in a solid-liquid ratio of 1 g:15 mL, ultrasonically extract at 80°C for 30 min, filter, and dry the filtrate in a vacuum at 60°C for 8 to 10 h, grind and pass through a 60-mesh sieve to obtain the licorice extract.

[0116] The raw materials prepared above are mixed uniformly according to the following mass proportions to obtain a traditional Chinese medicine feed additive that can replace antibiotics.

[0117] Example 3

[0118] A feed additive that can be used to replace antibiotics (a feed additive for livestock and poultry made from Chinese herbal medicine extracts that can replace antibiotics), the components of which are as follows, measured by weight:

[0119] 36 parts of Astragalus extract, 22 parts of Dandelion extract, 15 parts of Polygonatum sibiricum extract, 20 parts of Patchouli extract, 18 parts of Scutellaria baicalensis extract, 18 parts of Chrysanthemum indica extract, 15 parts of Magnolia officinalis extract, 15 parts of Isatis indigotica extract, 12 parts of Crataegus pinnatifida extract, 16 parts of Cannabis sativa seed extract, 15 parts of Honeysuckle extract, 12 parts of Isatis indigotica extract, 15 parts of Artemisia annua extract, 14 parts of Schisandra chinensis extract, 9 parts of Pulsatilla striata extract, 10 parts of Galla chinensis extract and 15 parts of Licorice extract.

[0120] The preparation method of the feed additive comprises the following specific steps:

[0121] Astragalus extract: After the astragalus is dried, it is crushed and passed through a 60-mesh sieve. Distilled water is added in a ratio of 1g:10mL. The mixture is boiled at 100°C for 3 hours and then filtered. The filtrate is concentrated to 50% of the original volume. 90% ethanol in a volume 4 times that of the concentrate is added and mixed evenly. The mixture is extracted at 4°C for 12 hours. After that, the precipitate is centrifuged at 10000r / min for 10 minutes to obtain the precipitate. The precipitate is vacuum dried at 70°C for 7 to 9 hours and crushed and passed through a 60-mesh sieve to obtain the astragalus extract.

[0122] Dandelion extract: Dry the dandelion, crush it through a 40-mesh sieve, add distilled water at a solid-liquid ratio of 1g:25mL, soak at room temperature for 24 hours, ultrasonically treat at 120W and 60℃ for 2 hours, filter, freeze-dry the filtrate at -55 to -60℃ for 24 to 30 hours, and then crush it through a 60-mesh sieve to obtain the dandelion extract.

[0123] Polygonatum sibiricum extract: Dry and crush the polygonatum sibiricum, pass through a 40-mesh sieve, add 75% ethanol in a ratio of 1g:45mL, ultrasonicate at 300W and 37℃ for 30min, ultrasonicate 4 times, filter, vacuum dry the filtrate at 60℃ for 8-10h, crush and pass through a 60-mesh sieve to obtain the polygonatum sibiricum extract.

[0124] Patchouli extract: Dry and crush the Patchouli, pass through a 40-mesh sieve, add petroleum ether in a solid-liquid ratio of 1g:8mL, extract at 50°C for 4 times, each time for 24h, concentrate on a rotary evaporator, dry naturally, crush and pass through a 60-mesh sieve to obtain the Patchouli extract.

[0125] Scutellaria baicalensis extract: Dry and crush the scutellaria baicalensis, pass through a 40-mesh sieve, add 75% ethanol in a solid-liquid ratio of 1g:10mL, extract twice under reflux at 80°C for 2 hours each time, filter, freeze-dry the filtrate at -55 to -60°C for 24 to 30 hours, crush and pass through a 60-mesh sieve to obtain the scutellaria baicalensis extract.

[0126] Wild chrysanthemum extract: dry and crush the wild chrysanthemum, pass through a 40-mesh sieve, add 75% ethanol in a solid-liquid ratio of 1g:20mL, extract at 60°C for 2h, filter, and vacuum dry the filtrate at 60°C for 8-10h, crush and pass through a 60-mesh sieve to obtain the wild chrysanthemum extract.

[0127] Magnolia bark extract: Dry and crush the Magnolia bark, pass through a 40-mesh sieve, add 75% ethanol in a solid-liquid ratio of 1 g:20 mL, microwave at 70°C and 240 W for 7 min, filter, vacuum dry the filtrate at 60°C for 8 to 10 h, crush and pass through a 60-mesh sieve to obtain the Magnolia bark extract.

[0128] Folium Isatidis Extract: Dry and crush the Folium Isatidis, pass through a 40-mesh sieve, add 80% methanol in a ratio of 1 g:25 mL, microwave at 60°C, 1000 W for 5 min, filter, vacuum dry the filtrate at 60°C for 8-10 h, crush and pass through a 60-mesh sieve to obtain the Folium Isatidis Extract.

[0129] Hawthorn extract: The hawthorn is dried and crushed, passed through a 40-mesh sieve, 5% of the mass of the crushed hawthorn by cellulase and 6% of the mass of the crushed hawthorn by pectinase are added, and enzymolysis is carried out at 55°C and pH 6.5 for 2 hours to obtain enzymolysis hawthorn powder; then, 75% ethanol is added at a solid-liquid ratio of 1g:35mL, ultrasonication is carried out at 40°C for 30 minutes, and filtration is carried out. The filtrate is vacuum dried at 60°C for 8-10 hours, crushed and passed through a 60-mesh sieve to obtain the hawthorn extract.

[0130] Hemp seed extract: Dry and crush the hemp seed, pass through a 60-mesh sieve, add petroleum ether at a solid-liquid ratio of 1g:12mL, condense and reflux at 60°C for 6h, let stand for 30min and then filter, extract the residue again once, dry the obtained extract at 60°C in vacuum for 8-10h, crush and pass through a 60-mesh sieve to obtain the hemp seed extract.

[0131] Honeysuckle extract: Dry and crush the honeysuckle, pass through a 40-mesh sieve, add 80% ethanol in a solid-liquid ratio of 1 g:10 mL, extract at 80°C under reflux for 2 times, each time for 2 hours, filter, and vacuum dry the filtrate at 60°C for 8 to 10 hours to obtain the honeysuckle extract.

[0132] Radix Isatidis Extract: Dry and crush the Radix Isatidis, pass through a 40-mesh sieve, add 75% ethanol in a solid-liquid ratio of 1 g:10 mL, extract at 90°C for 30 min, filter, freeze-dry the filtrate at -55 to -60°C for 24 to 30 h, crush and pass through a 60-mesh sieve to obtain the Radix Isatidis Extract.

[0133] Artemisia annua extract: Dry and crush the Artemisia annua leaves, pass through a 40-mesh sieve, add petroleum ether in a solid-liquid ratio of 1 g:20 mL, extract at 50°C for 2.5 h, filter at room temperature, concentrate under reduced pressure, add 2 wt.% NaOH solution for washing and filtration, wash 4 times with distilled water, concentrate on a rotary evaporator, and vacuum dry the resulting concentrate at 60°C for 8 to 10 h, crush and pass through a 60-mesh sieve to obtain the Artemisia annua extract.

[0134] Schisandra extract: Dry and crush the schisandra, pass through a 40-mesh sieve, add 80% ethanol in a solid-liquid ratio of 1g:8mL, extract at 80°C for 40min, filter the filtrate and dry it in a vacuum at 60°C for 8-10h, crush and pass through a 60-mesh sieve to obtain the schisandra extract.

[0135] Pulsatilla extract: Crush the Pulsatilla, pass through a 60-mesh sieve, add distilled water in a solid-liquid ratio of 1g:15mL, extract for 3 times, each extraction time is 5h, and the extraction temperature is 80℃; combine the filtrate, vacuum dry the filtrate at 60℃ for 8-10h, crush and pass through a 60-mesh sieve to obtain the Pulsatilla extract.

[0136] Gallnut extract: Crush the gallnut, pass through a 40-mesh sieve, add a mixture of 75% ethanol and 1.0wt.% hydrochloric acid in a solid-liquid ratio of 1g:25mL (the volume ratio of 75% ethanol and 1.0wt.% hydrochloric acid in the mixture is 65:1), ultrasonically extract at 50°C for 1.5h, filter the filtrate and vacuum dry it at 60°C for 8-10h, crush and pass through a 60-mesh sieve to obtain the gallnut extract.

[0137] Licorice extract: Grind the licorice, pass through a 90-mesh sieve, add 60% ethanol in a solid-liquid ratio of 1 g:15 mL, ultrasonically extract at 80°C for 30 min, filter, vacuum dry the filtrate at 60°C for 8-10 h, grind and pass through a 60-mesh sieve to obtain the licorice extract.

[0138] The raw materials prepared above are mixed uniformly according to the following mass proportions to obtain a traditional Chinese medicine feed additive that can replace antibiotics.

[0139] Example 4 Test results of Chinese herbal medicine extract feed additives for livestock and poultry that replace antibiotics on Newcastle disease antibody titer in broilers

[0140] 720 healthy AA broilers at 1 day old were selected and randomly divided into 4 groups, denoted as Group I-Group IV, with 180 chickens in each group, 3 replicates in each group, and 60 chickens in each replicate. Group I (control group) was fed with a basic diet, and Group II, Group III and Group IV were fed with a feed additive prepared in Example 1 (a feed additive for livestock and poultry Chinese medicine extracts that replaces antibiotics) added to the basic diet, respectively, and the addition amount accounted for 0.5%, 1.0% and 2.0% of the weight of the basic diet, respectively, mixed evenly, and fed freely. On the 7th day, chicken Newcastle disease (ND) IV vaccine was inoculated with nasal drops and eye drops, and chicken Newcastle disease IV vaccine was injected intramuscularly on the 14th day, and the test period was 42d. At 28 and 42 days of age, 20 chickens were randomly selected from each group, 1 mL of blood was collected from the subwing vein, and serum was separated. The titer of Newcastle disease antibody was detected by hemagglutination and hemagglutination inhibition test (HA-HI), and the test results are shown in Table 1.

[0141] Table 1 Test results of Chinese herbal medicine extract feed additives for livestock and poultry that replace antibiotics on the titer of Newcastle disease antibodies in broilers

[0142]

[0143]

[0144] Note: Data in the same column with different capital letters on shoulders indicate extremely significant differences (P<0.01), data with different lowercase letters on shoulders indicate significant differences (P<0.05), and data with the same letters on shoulders or no letters on shoulders indicate no significant differences (P>0.05); the same as in the following tables.

[0145] As shown in Table 1, at 28 days of age, the NDV antibody levels of group III and group IV were significantly higher than those of the control group (P < 0.01), and group II was significantly higher than the control group (P < 0.5); at 42 days of age, the NDV antibody level of group III was significantly higher than that of the control group (P < 0.01), and the NDV antibody levels of group II and group IV were significantly higher than those of the control group (P < 0.05). It can be seen that adding the feed additive prepared in Example 1 to the basic diet can increase the NDV antibody level of broilers.

[0146] Example 5 Test results of Chinese herbal medicine extract feed additives for livestock and poultry that replace antibiotics on swine fever antibodies

[0147] 120 28-day-old "Du × Chang × Da" three-way crossbred piglets with similar body weight were selected and randomly divided into 4 groups, recorded as Group I-Group IV, with 30 piglets in each group, 3 replicates in each group, and 10 piglets in each replicate. At 28 days of age, 1 dose of swine fever rabbit attenuated vaccine was injected per head. Group I (control group) was fed with a basic diet, and Group II, Group III and Group IV were fed with the feed additive prepared in Example 1 added to the basic diet, respectively, in an amount of 1%, 2% and 4% of the mass of the basic diet, mixed evenly, and fed freely. The test started at 28 days of age and ended at 64 days of age, with a test period of 36 days. At 42 days of age, 49 days of age, 56 days of age and 64 days of age, 3 piglets were randomly selected from each group, with 1 pig in each replicate, 6 mL of blood was collected from the anterior vena cava, centrifuged at 3000 r / min for 10 minutes, and serum was prepared. The test was strictly determined according to the instructions of the swine fever ELISA antibody detection kit, and the test results are shown in Table 2.

[0148] Table 2 Test results of Chinese herbal medicine extract feed additives for livestock and poultry that replace antibiotics on swine fever antibodies

[0149]

[0150] As shown in Table 2, at 42 days of age, the levels of classical swine fever antibodies in Group III and Group IV were significantly higher than those in the control group (P < 0.01), and the level of classical swine fever antibodies in Group II was higher than that in the control group, but the difference was not significant (P > 0.05). At 49, 56 and 64 days of age, the level of classical swine fever antibodies in Group III was significantly higher than that in the control group (P < 0.01), and the levels of classical swine fever antibodies in Group II and Group IV were significantly higher than those in the control group (P < 0.05). It can be seen that adding the feed additive prepared in Example 1 to the basic diet can increase the level of classical swine fever antibodies.

[0151] Example 6 Test results of Chinese herbal medicine extract feed additives for livestock and poultry that replace antibiotics on the average daily feed intake, average daily weight gain and feed-to-meat ratio of broilers

[0152] 720 healthy AA broilers of 1 day old were selected and randomly divided into 4 groups, recorded as Group I-Group IV, with 180 chickens in each group, 3 replicates in each group, and 60 chickens in each replicate. Group I (control group) was fed with a basal diet, and Group II, Group III and Group IV were fed with the feed additive prepared in Example 1 added to the basal diet, and the addition amount accounted for 0.5%, 1.0% and 2.0% of the basal diet mass, respectively, mixed evenly, and fed freely. During the test, the feed consumption was accurately weighed and recorded every day. On the 42nd day of the test, the experimental chickens were weighed on an empty stomach, the feed consumption during the test period was counted, and the average daily weight gain, average daily feed intake and feed-to-meat ratio of the entire test period were calculated. The statistical results are shown in Table 3.

[0153] Table 3 Test results of Chinese herbal medicine extract feed additives for livestock and poultry that replace antibiotics on the average daily feed intake, average daily weight gain and feed-to-meat ratio of broilers

[0154]

[0155] As shown in Table 3, the average daily feed intake of broilers in Group II, Group III and Group IV was higher than that in the control group, but the difference was not significant (P>0.05). The average daily weight gain of broilers in Group II, Group III and Group IV was significantly higher than that in the control group (P<0.05). The feed-to-meat ratio of broilers in Group III was significantly lower than that in the control group (P<0.01), and both Group II and Group IV were significantly lower than the control group (P<0.05). It can be seen that adding the feed additive prepared in Example 1 to the basal diet can increase the average daily weight gain of broilers and reduce the feed-to-meat ratio.

[0156] Example 7 Test results of Chinese herbal medicine extract feed additives for livestock and poultry that replace antibiotics on the average daily feed intake, average daily weight gain and feed-to-meat ratio of pigs

[0157] 120 "Du × Chang × Da" three-way hybrid pigs (weighing about 60kg) were selected and randomly divided into 4 groups, recorded as Group I-Group IV, 30 pigs in each group, 3 replicates in each group, and 10 pigs in each replicate. Group I (control group) was fed with a basic diet, and Group II, Group III and Group IV were fed with the feed additive prepared in Example 1 added to the basic diet, the addition amount accounted for 1%, 2% and 4% of the basic diet mass, respectively, mixed evenly, and fed freely. The test period was 60 days, and the initial and final weights of the pigs in the test period and the feed consumption during the fattening period were recorded, and the average daily feed intake, average daily weight gain and feed-to-meat ratio were calculated. The statistical results are shown in Table 4.

[0158] Table 4 Test results of Chinese herbal medicine extract feed additives for livestock and poultry that replace antibiotics on the average daily feed intake, average daily weight gain and feed-to-meat ratio of pigs

[0159]

[0160] As can be seen from Table 4, the weight of pigs in group III at the end of the test period was significantly higher than that in the control group (P < 0.01), and the weight of pigs in groups II and IV at the end of the test period was significantly higher than that in the control group (P < 0.05). The average daily feed intake of pigs in groups II, III and IV was higher than that in the control group, but the difference was not significant (P > 0.05). The average daily weight gain of pigs in groups II, III and IV was significantly higher than that in the control group (P < 0.01). The feed-to-meat ratio of group III was significantly lower than that of the control group (P < 0.01), and the feed-to-meat ratio of groups II and IV was significantly lower than that of the control group (P < 0.05). It can be seen that the feed additive prepared in Example 1 can increase the average daily weight gain and final weight of pigs and reduce the feed-to-meat ratio.

[0161] Example 8 Detection results of Chinese herbal medicine extract feed additives for livestock and poultry replacing antibiotics on IL-1β, IL-6, IFN-γ and TNF-α in broiler serum

[0162] 720 healthy AA broilers at one day of age were selected and randomly divided into 4 groups, designated as Group I-Group IV, with 180 chickens in each group, 3 replicates in each group, and 60 chickens in each replicate. Group I (control group) was fed with a basal diet, Group II was fed with a basal diet + 2% of the mass of the basal diet prepared by the feed additive in Example 1, Group III (model group) was fed with a basal diet + 500 μg / kg body weight of LPS injected, and Group IV was fed with a basal diet + 2% of the mass of the basal diet prepared by Example 1 + 500 μg / kg body weight of LPS injected; the feeds were mixed evenly and fed freely. On the 15th and 21st days, Group III and Group IV were injected with 500 μg / kg body weight of LPS on an empty stomach, respectively, and Group I and Group II were injected with an equal volume of normal saline on an empty stomach, respectively. The experimental period was 28 days. After the experiment, 9 broilers were randomly selected from each group, with 3 broilers in each replicate. 2 mL of blood was collected from the wing vein of each broiler, and the serum was separated by centrifugation. IL-1β, IL-6, IFN-γ and TNF-α were determined according to the instructions of the ELISA kit. The results are shown in Table 5.

[0163] Table 5 Detection results of Chinese herbal medicine extract feed additives for livestock and poultry that replace antibiotics on broiler serum IL-1β, IL-6, IFN-γ and TNF-α

[0164]

[0165] As shown in Table 5, without LPS injection, the content of cytokines IL-1β, IL-6 and TNF-α in the serum of broilers in group II was significantly lower than that in the control group (P < 0.05), and the content of IFN-γ in group II was significantly higher than that in the control group (P < 0.05). After LPS injection, the content of cytokine IL-1β in the serum of broilers in group IV was significantly lower than that in the model group (P < 0.05), the content of IL-6 and TNF-α in group IV was significantly lower than that in the model group (P < 0.01), and the content of IFN-γ in group IV was significantly higher than that in the model group (P < 0.05). It can be seen that the feed additive prepared in Example 1 can reduce the content of cytokines IL-1β, IL-6 and TNF-α in broiler serum and increase the content of IFN-γ.

[0166] Example 9 Test results of Chinese herbal medicine extract feed additives for livestock and poultry as an alternative to antibiotics on the survival rate of pigs and the incidence of digestive and respiratory diseases

[0167] The experimental animals were 372 fattening pigs of "Du×Chang×Da" three-way crossbreed weighing 9-12 kg from three pig farms in a certain place, divided into 4 groups, recorded as Group I-Group IV, 93 pigs in each group, 3 replicates in each group, and 31 pigs in each replicate. Group I (control group) was fed with a basic diet, and Group II, Group III and Group IV were fed with the feed additive prepared in Example 1 added to the basic diet, the added amount accounted for 1%, 2% and 4% of the weight of the basic diet, respectively, mixed evenly, and fed freely. The experimental period was 120 days. The pig survival rate, fecal abnormality rate and respiratory abnormality rate were statistically analyzed, and the results are shown in Table 6.

[0168] Table 6 Test results of Chinese herbal medicine extract feed additives for livestock and poultry that replace antibiotics on the survival rate of pigs, the incidence of digestive tract and respiratory diseases

[0169]

[0170]

[0171] As can be seen from Table 6, the survival rates of pigs in Group II, Group III and Group IV were significantly higher than those in the control group (P < 0.01). The abnormal feces rates of pigs in Group III and Group IV were significantly lower than those in the control group (P < 0.01), and the abnormal feces rates of pigs in Group II were significantly lower than those in the control group (P < 0.05). The respiratory tract abnormality rates of Group III and Group IV were significantly lower than those in the control group (P < 0.05), and the respiratory tract abnormality rate of Group II was lower than that in the control group, but the difference was not significant (P > 0.05). It can be seen that the feed additive prepared in Example 1 can improve the survival rate of pigs and reduce the abnormal feces rates and respiratory tract abnormality rates.

[0172] Example 10 Effect of Chinese herbal medicine extract feed additives replacing antibiotics on the tissue structure and flora of jejunal mucosa of broiler chickens

[0173] 720 healthy AA broilers of 1 day old were selected and randomly divided into 4 groups, denoted as Group I-Group IV, with 180 chickens in each group, 3 replicates in each group, and 60 chickens in each replicate. Group I (control group) was fed with a basal diet, and Group II, Group III and Group IV were fed with the feed additive prepared in Example 1 added to the basal diet, the addition amount accounted for 0.5%, 1.0% and 2.0% of the basal diet mass, respectively, mixed evenly, and fed freely. Three chickens were randomly selected from each group at 42 days of age, and one chicken was selected from each replicate. The neck was bled to death, the abdominal cavity was opened, the jejunum was separated, about 2 to 3 cm was cut from the middle part of the jejunum, and the contents were quickly immersed in physiological saline to rinse, and then placed in 10% formaldehyde fixative to make tissue sections. After HE staining, a fluorescent inverted microscope and a microscopic image analysis system were used to measure the intestinal villus height (V) and crypt depth (C), and the villus height / crypt depth (V / C) were calculated. Three chickens were randomly selected from each group at 42 days of age, with one chicken in each replicate. They were killed by bleeding from the neck, and the contents of the mid-ileum were quickly collected and frozen in liquid nitrogen. The number of Escherichia coli, Lactobacillus and Bifidobacterium colonies was detected by colony counting method using specific culture media (MacConkey, MRS, BLB). The results are shown in Tables 7 and 8.

[0174] Table 7 Effects of Chinese herbal medicine extract feed additives replacing antibiotics on the jejunal mucosal tissue structure of broiler chickens

[0175]

[0176] As shown in Table 7, the height of jejunal villi of broiler chickens in groups II, III and IV was significantly higher than that in the control group (P < 0.01). The depth of jejunal crypts of broiler chickens in groups II, III and IV was significantly higher than that in the control group (P < 0.05). The height of jejunal villi and the depth of crypts of broiler chickens in groups II, III and IV were not significantly different from those in the control group (P > 0.05). It can be seen that the feed additive prepared in Example 1 can increase the height of jejunal villi and the depth of crypts of broiler chickens.

[0177] Table 8 Effects of Chinese herbal medicine extract feed additives replacing antibiotics on ileal flora of broiler chickens

[0178]

[0179]

[0180] As can be seen from Table 8, the number of Escherichia coli in the ileum of broiler chickens in groups III and IV was significantly lower than that in the control group (P < 0.01), and the number of Escherichia coli in the ileum of broiler chickens in group II was significantly lower than that in the control group (P < 0.05). The number of Bifidobacterium in the ileum of broiler chickens in groups III and IV was significantly higher than that in the control group (P < 0.01), and the number of Bifidobacterium in the ileum of broiler chickens in group II was significantly higher than that in the control group (P < 0.05). The number of Lactobacillus in the ileum of broiler chickens in groups II, III and IV was significantly higher than that in the control group (P < 0.01). It can be seen that the feed additive prepared in Example 1 can reduce the number of Escherichia coli in the ileum of broiler chickens and increase the number of Bifidobacterium and Lactobacillus.

[0181] Example 11 Detection results of Chinese herbal medicine extract feed additives for livestock and poultry that replace antibiotics on pig jejunal mucosal tissue structure and rectal flora

[0182] 120 fattening pigs of "Du×Chang×Da" three-way cross with a body weight of about 40 kg were selected and randomly divided into 4 groups, 30 pigs in each group, 3 replicates in each group, and 10 pigs in each replicate. Group I (control group) was fed with a basal diet, and groups II, III and IV were fed with the feed additive prepared in Example 1 added to the basal diet, respectively, in an amount of 1%, 2% and 4% of the mass of the basal diet, respectively. The added amount accounted for 1%, 2% and 4% of the mass of the basal diet, respectively. The pigs were mixed evenly and fed freely. The test period was 90 days. On the 60th day of the test, 3 pigs were randomly selected from each group, 1 pig in each replicate, and fecal samples were collected in an aseptic rectum and sterilized and stored at low temperature. After the test, 3 pigs were randomly selected from each group, 1 pig in each replicate, and the jejunum was taken after slaughter. It was fixed with 4% paraformaldehyde, paraffin sections were made, and microscopic analysis was performed to measure the height of intestinal villi and the depth of crypts, and the villus-crypt ratio was calculated. MRS medium and MacConkey agar medium were plated to count lactic acid bacteria, Escherichia coli and Bifidobacterium in fecal samples, respectively. The bacterial count was expressed as lg CFU / g. The results are shown in Tables 9 and 10.

[0183] Table 9 Test results of Chinese herbal medicine extract feed additives for livestock and poultry replacing antibiotics on the jejunal mucosal tissue structure of pigs

[0184]

[0185] As shown in Table 9, the height of the jejunal villi of pigs in Group II, Group III and Group IV was significantly higher than that in the control group (P < 0.05). The depth of the jejunal crypts of pigs in Group III was extremely significantly higher than that in the control group (P < 0.01), and the depth of the jejunal crypts of pigs in Group II and Group IV were significantly higher than those in the control group (P < 0.05). The ratio of the jejunal villi height to the crypt depth of pigs in Group II, Group III and Group IV was higher than that in the control group, but the differences were not significant (P > 0.05). It can be seen that the feed additive prepared in Example 1 can increase the height of the jejunal villi and the crypt depth of pigs.

[0186] Table 10 Test results of Chinese herbal medicine extract feed additives for livestock and poultry that replace antibiotics on pig rectal flora

[0187]

[0188]

[0189] As can be seen from Table 10, the number of Escherichia coli in the pig rectum of groups III and IV was significantly lower than that of the control group (P < 0.01), and the number of Escherichia coli in the pig rectum of group II was significantly lower than that of the control group (P < 0.05). The number of Bifidobacterium in the pig rectum of groups II, III and IV was significantly higher than that of the control group (P < 0.05). The number of Lactobacillus in the pig rectum of group III was significantly higher than that of the control group (P < 0.01), and the number of Lactobacillus in the pig rectum of groups II and IV was significantly higher than that of the control group (P < 0.05). It can be seen that the feed additive prepared in Example 1 can reduce the number of Escherichia coli in the pig rectum and increase the number of Bifidobacterium and Lactobacillus.

[0190] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A combination of Chinese herbal extracts that can be used to replace antibiotics, characterized in that: The Chinese medicine extract combination comprises the following components in parts by weight: 25-36 parts of Astragalus extract, 15-22 parts of Dandelion extract, 15-26 parts of Polygonatum sibiricum extract, 15-20 parts of Patchouli extract, 12-18 parts of Scutellaria baicalensis extract, 10-18 parts of Chrysanthemum indica extract, 12-19 parts of Magnolia officinalis extract, 10-18 parts of Isatis indigotica extract, 12-18 parts of Crataegus pinnatifida extract, 7-16 parts of Cannabis sativa seed extract, 5-15 parts of Honeysuckle extract, 5-12 parts of Isatis indigotica extract, 6-15 parts of Artemisia annua extract, 6-14 parts of Schisandra chinensis extract, 7-12 parts of Pulsatilla striata extract, 5-10 parts of Galla chinensis extract and 12-17 parts of Licorice extract.

2. The Chinese herbal medicine extract combination according to claim 1, characterized in that: The Chinese medicine extract combination comprises the following components in parts by weight: 25 parts of Astragalus extract, 15 parts of Dandelion extract, 17 parts of Polygonatum sibiricum extract, 15 parts of Patchouli extract, 16 parts of Scutellaria baicalensis extract, 16 parts of Chrysanthemum indica extract, 12 parts of Magnolia officinalis extract, 10 parts of Isatis indigotica extract, 17 parts of Crataegus pinnatifida extract, 7 parts of Cannabis sativa seed extract, 8 parts of Honeysuckle extract, 5 parts of Isatis indigotica extract, 6 parts of Artemisia annua extract, 6 parts of Schisandra chinensis extract, 7 parts of Pulsatilla striata extract, 5 parts of Galla chinensis extract and 17 parts of Licorice extract.

3. The Chinese herbal medicine extract combination according to claim 1, characterized in that: The preparation method of the astragalus extract comprises the steps of crushing, water extraction, alcohol extraction, centrifugation and drying the astragalus in sequence to obtain the astragalus extract; The preparation method of the dandelion extract comprises the steps of sequentially crushing, water extraction, ultrasonication, filtration and freeze drying the dandelion to obtain the dandelion extract; The preparation method of the polygonatum sibiricum extract comprises the steps of sequentially crushing polygonatum sibiricum, ultrasonic alcohol extraction, filtering and vacuum drying to obtain the polygonatum sibiricum extract; The preparation method of the Patchouli extract comprises the steps of crushing Patchouli in sequence, extracting with petroleum ether and concentrating the Patchouli to obtain the Patchouli extract; The preparation method of the scutellaria baicalensis extract comprises the steps of sequentially crushing, alcohol extraction, filtering and freeze-drying the scutellaria baicalensis to obtain the scutellaria baicalensis extract; The method for preparing the wild chrysanthemum extract comprises the steps of sequentially crushing the wild chrysanthemum, extracting with alcohol, filtering and vacuum drying to obtain the wild chrysanthemum extract; The preparation method of the Magnolia bark extract comprises the steps of sequentially crushing the Magnolia bark, performing microwave alcohol extraction, filtering and vacuum drying to obtain the Magnolia bark extract; The preparation method of the isatis indigo leaf extract comprises the steps of sequentially crushing the isatis indigo leaf, performing microwave alcohol extraction, filtering and vacuum drying to obtain the isatis indigo leaf extract; The preparation method of the hawthorn extract comprises the steps of sequentially crushing hawthorn, enzymolysis, ultrasonic alcohol extraction, filtration and vacuum drying to obtain the hawthorn extract; The preparation method of the hemp seed extract comprises the steps of crushing the hemp seed in sequence, extracting with petroleum ether, filtering and freeze-drying to obtain the hemp seed extract; The preparation method of the honeysuckle extract comprises the steps of sequentially crushing honeysuckle, alcohol extraction, filtering and vacuum drying to obtain the honeysuckle extract; The preparation method of the Radix Isatidis extract comprises the steps of sequentially crushing the Radix Isatidis, extracting with alcohol, filtering and vacuum drying the Radix Isatidis to obtain the Radix Isatidis extract; The preparation method of the artemisia annua extract comprises the steps of sequentially crushing the artemisia annua, extracting with petroleum ether, washing with a NaOH solution, filtering, concentrating and vacuum drying to obtain the artemisia annua extract; The preparation method of the Schisandra chinensis extract comprises the steps of sequentially crushing, alcohol extracting, filtering and vacuum drying the Schisandra chinensis to obtain the Schisandra chinensis extract; The preparation method of the Pulsatilla chinensis extract comprises the steps of sequentially crushing, water extracting, filtering and vacuum concentrating the Pulsatilla chinensis to obtain the Pulsatilla chinensis; The preparation method of the gallnut extract comprises the steps of sequentially crushing the gallnut, ultrasonically extracting with alcohol, filtering and vacuum drying the gallnut to obtain the gallnut extract; The preparation method of the licorice extract comprises the steps of sequentially crushing licorice, ultrasonic alcohol extraction, suction filtration, filtration and vacuum drying to obtain the licorice extract.

4. The Chinese herbal medicine extract combination according to claim 3, characterized in that: When preparing the astragalus extract, the solid-liquid ratio of the water extraction is 1g: (8-10)mL, the temperature is 100°C, and the time is 3h; the solvent used in the alcohol extraction is 90% ethanol, the temperature is 4°C, and the time is 12h; the speed of the centrifugation is 8000-10000r / min, and the time is 10min; When preparing the dandelion extract, the water extraction material-liquid ratio is 1g: (20-25)mL, and the time is 20-24h; the power of the ultrasound is 120W, the temperature is 60°C, and the time is 1.5-2h; When preparing the polygonatum sibiricum extract, the solvent used in the ultrasonic alcohol extraction is 75% ethanol; the material-liquid ratio of the ultrasonic alcohol extraction is 1g: (40-45)mL, the temperature is 37°C, the power is 300W, the number of ultrasonic times is 3-4 times, and the time of each ultrasonic time is 30min; When preparing the Patchouli extract, the material-liquid ratio of the petroleum ether extraction is 1 g: (5-8) mL, the temperature is 50° C., the number of times is 3-4 times, and the time of each petroleum ether extraction is 12-24 hours; When preparing the scutellaria baicalensis extract, the solvent used in the alcohol extraction is 75% ethanol; the solid-liquid ratio of the alcohol extraction is 1g: (8-10)mL, the temperature is 80°C, the number of times is 2, and the time of each alcohol extraction is 1.5-2h; When preparing the wild chrysanthemum extract, the solvent used in the alcohol extraction is 75% ethanol; the solid-liquid ratio of the alcohol extraction is 1g: (18-20)mL, the temperature is 60°C, and the time is 1.5-2h; When preparing the Magnolia officinalis extract, the solvent used in the microwave alcohol extraction is 75% ethanol; the material-liquid ratio of the microwave alcohol extraction is 1g: (18-20)mL, the temperature is 70°C, the power is 240W, and the time is 5-7min; When preparing the isatis indigofera extract, the solvent used in the microwave alcohol extraction is 80% ethanol; the material-liquid ratio of the microwave extraction is 1g: (20-25)mL, the temperature is 60°C, the power is 1000W, and the time is 3-5min; When preparing the hawthorn extract, the enzymes used in the enzymatic hydrolysis are cellulase and pectinase; the amount of the cellulase used is 4% to 5% of the mass of the hawthorn obtained after crushing; the amount of the pectinase used is 5% to 6% of the mass of the hawthorn obtained after crushing; the temperature of the enzymatic hydrolysis is 55°C, the pH is 5.5 to 6.5, and the time is 1.5 to 2h; the solvent used in the ultrasonic alcohol extraction is 75% ethanol; the material-liquid ratio of the ultrasonic alcohol extraction is 1g: (30 to 35)mL, the temperature is 40°C, and the time is 30min; When preparing the hemp seed extract, the material-liquid ratio of the petroleum ether extraction is 1g: (8-12)mL, the temperature is 60°C, and the time is 6h; When preparing the honeysuckle extract, the solvent used in the alcohol extraction is 80% ethanol; the solid-liquid ratio of the alcohol extraction is 1g: (8-10)mL, the temperature is 80°C, the number of times is 2, and the time of each alcohol extraction is 1.5-2h; When preparing the Radix Isatidis extract, the solvent used in the alcohol extraction is 75% ethanol; the solid-liquid ratio of the alcohol extraction is 1g: (8-10)mL, the temperature is 90°C, and the time is 30min; When preparing the Artemisia annua extract, the material-liquid ratio of the petroleum ether extraction is 1 g: (18-20) mL, the temperature is 50° C., and the time is 2-2.5 h; When preparing the Schisandra chinensis extract, the solvent used in the alcohol extraction is 80% ethanol; the solid-liquid ratio of the alcohol extraction is 1g: (6-8)mL, the temperature is 80°C, and the time is 30-40min; When preparing the Pulsatilla chinensis extract, the water extraction solid-liquid ratio is 1 g: (10-15) mL, the temperature is 80° C., the number of times is 3, and the time of each water extraction is 5 hours; When preparing the gallnut extract, the solvent used in the ultrasonic alcohol extraction is a mixed solution of 75% ethanol and 1% acetic acid; the volume of 75% ethanol and 1% acetic acid in the mixed solution is 65:1; the solid-liquid ratio of the alcohol extraction is 1g: (20-25)mL, the temperature is 50°C, and the time is 1-1.5h; When preparing the licorice extract, the solvent used in the ultrasonic extraction is 60% ethanol; the solid-liquid ratio of the alcohol extraction is 1g:15mL, the temperature is 80°C, and the time is 30min.

5. Use of the Chinese herbal extract combination according to any one of claims 1 to 4 in the preparation of a feed additive that can be used to replace antibiotics.

6. A feed additive that can be used to replace antibiotics, characterized in that: The feed additive comprises the combination of Chinese medicinal extracts according to any one of claims 1 to 4.

7. The method for preparing the feed additive according to claim 6, characterized in that: The preparation method comprises the following steps: The astragalus extract, the dandelion extract, the polygonatum extract, the patches extract, the scutellaria extract, the wild chrysanthemum extract, the magnolia bark extract, the isatis indigotica extract, the hawthorn extract, the hemp seed extract, the honeysuckle extract, the isatis root extract, the artemisia annua extract, the schisandra chinensis extract, the pulsatilla extract, the gallnut extract and the liquorice extract are mixed evenly to obtain the feed additive.

8. Use of the Chinese medicinal extract combination according to any one of claims 1 to 4 or the feed additive according to claim 6 in preparing feed that can be used to replace antibiotics.

9. A feed that can be used to replace antibiotics, characterized in that: The feed comprises the feed additive according to claim 6 and a basic diet.

10. Use of the Chinese medicinal extract combination according to any one of claims 1 to 4, the feed additive according to claim 6 or the feed according to claim 8 in any one of the following: (1) Application in improving the immunity of livestock and poultry; (2) Application in the preparation of products for improving the immunity of livestock and poultry; (3) Application in promoting the growth of livestock and poultry; (4) Application in the preparation of products that promote the growth of livestock and poultry; (5) Application in improving the survival rate of livestock and poultry; (6) Application in the preparation of products for improving the survival rate of livestock and poultry; (7) Application in improving the jejunal mucosal tissue structure of livestock and poultry; (8) Application in the preparation of products for improving the jejunal mucosal tissue structure of livestock and poultry; (9) Application in improving the intestinal flora structure of livestock and poultry; (10) Application in the preparation of products for improving the intestinal flora structure of livestock and poultry.

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