Brassica napus seed oil content gene soca9 and related molecular markers

By cloning the oil content gene SOCA9 in rapeseed seeds and its molecular markers, the problem of weak oil content regulation in existing technologies has been solved, achieving breeding results with high oil content, low lignocellulose and stable fatty acid composition, and providing an efficient breeding tool.

CN120005893BActive Publication Date: 2026-06-02HUAZHONG UNIV OF SCI & TECH
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Patent Information

Application Number
CN202311521958.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-11-15
Publication Date
2026-06-02
Estimated Expiration
2043-11-15

AI Technical Summary

Technical Problem

In existing technologies, the gene regulation effect on oil content in rapeseed seeds is relatively weak, and it is difficult to simultaneously improve lignocellulose content and seed coat color, which limits the breeding potential for high oil content. Furthermore, transgenic breeding may alter the fatty acid composition of rapeseed oil.

Method used

The oil content gene SOCA9 of rapeseed and its related molecular markers were cloned and utilized. Through gene modification, complementation, knockout or silencing, the seed oil content, lignocellulose content and seed coat color were regulated. Molecular markers A9-M6 and A9-M7 closely linked to SOCA9 were developed for molecular marker-assisted selection breeding.

Benefits of technology

It significantly increases the oil content of Brassica napus seeds, reduces the lignocellulose content, and maintains the seed coat color and fatty acid composition of rapeseed oil, providing an efficient breeding tool and improving breeding efficiency and quality.

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Abstract

The application belongs to the technical field of molecular biology and genetic breeding, and discloses a Brassica napus seed oil content gene SOCA9 and related molecular markers, wherein the SOCA9 gene for regulating the seed oil content of Brassica napus has a nucleotide sequence as shown in SEQ ID No. 1 or a complementary sequence thereof, or a derived nucleotide sequence with not less than 95% homology with the nucleotide sequence as shown in SEQ ID No. 1 and the same function due to addition, deletion or replacement of one or more nucleotides. The gene SOCA9 with the nucleotide sequence as shown in SEQ ID No. 1 (or a complementary sequence thereof, a derived nucleotide sequence, etc.) can be used to improve the seed oil content of Brassica napus, reduce the seed lignocellulose content and make the seed coat color lighter, and can be used for high-oil-content Brassica napus transgenic breeding and molecular marker assisted screening breeding.
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