Compact fluorescence detection system and compact fluorescence detection system for immunochromatography

Abstract

The invention discloses a compact fluorescence detection system and a compact fluorescence detection system for immunochromatography. The system comprises an excitation light source device, a diaphragm device, a collimation flat top device, an excitation / emission beam splitting device, a light spot focusing lens, an emission optical filter, an emission focusing lens and a photoelectric detection device, the excitation light source device is used for emitting an excitation light beam with a specific wavelength; the diaphragm device is used for blocking a large-angle excitation light beam from passing through; the collimation flat-top device is used for angle collimation and light beam shaping of the excitation light beam; the excitation / emission beam splitting device is used for separating an excitation light beam and an emission light beam; the light spot focusing lens is used for focusing the excitation light beam into a light spot; the emission optical filter is used for filtering the excitation light signal; the emission focusing lens is used for converging emission light beams; the photoelectric detection device is used for receiving the emitted light beams and converting the emitted light beams into electric signals. The problems that a traditional fluorescence immunochromatography detection system is large in size, low in detection sensitivity and the like are solved.

Description

Technical Field

[0001] The present application relates to the field of in vitro diagnostic instruments, and in particular to a compact fluorescence detection system and a compact fluorescence detection system for immunochromatography. Background Art

[0002] Immunochromatographic Assay (ICA) is an analytical method developed in the late 20th century that combines immunoassay and chromatographic techniques. This method is specific, easy to operate, and rapid, and is widely used in important fields such as clinical diagnosis, environmental monitoring, and food safety. Traditional immunochromatographic technology uses colloidal gold as a marker to perform qualitative detection or semi-quantitative analysis of the target through strip color development. Although this method is simple and rapid, it has poor sensitivity and is difficult to accurately quantify. As a new type of immunoassay technology, fluorescent immunochromatography not only retains the advantages of traditional colloidal gold test strips for on-site rapid detection, but also incorporates the high sensitivity of fluorescent detection technology, becoming one of the main ways to improve the detection performance of immunochromatographic methods.

[0003] The traditional fluorescent immunochromatographic detection system is limited by the traditional DM spectroscopic optical path, and the overall vertical height is difficult to reduce. There are problems such as large overall volume and low detection sensitivity. Summary of the invention

[0004] The purpose of the embodiments of the present application is to provide a compact fluorescence detection system and a compact fluorescence detection system for immunochromatography, so as to improve the problems of traditional fluorescence immunochromatography detection systems such as large size and low detection sensitivity.

[0005] In the first aspect, an embodiment of the present application provides a compact fluorescence detection system for immunochromatography, comprising: an excitation light source device, an aperture device, a collimating flat-top device, an excitation / emission beam splitter device, a spot focusing lens, an emission filter, an emission focusing lens and a photoelectric detection device; the excitation light source device is used to emit an excitation light beam with a specific wavelength; the aperture device is used to block the passage of an excitation light beam at a large angle; the collimating flat-top device is used for angular collimation and beam shaping of the excitation light beam; the excitation / emission beam splitter device is used to separate the excitation light beam and the emission light beam; the spot focusing lens is used to focus the excitation light beam into a point spot or a line spot; the emission filter is used to filter the excitation light signal; the emission focusing lens is used to converge the emission light beam; the photoelectric detection device is used to receive the emission light beam and convert it into an electrical signal.

[0006] Further preferably, the compact fluorescence detection system comprises: an excitation light source device, an aperture device, a collimating flat-top device, a spot focusing lens, an emission filter, an emission focusing lens and a photoelectric detection device, and also comprises: an excitation / emission beam splitter; the excitation light source device is used to emit an excitation light beam; the aperture device is used to limit the aperture of the excitation light beam; the collimating flat-top device is used to collimate and shape the excitation light beam output by the aperture device; the excitation / emission beam splitter is used to vertically reflect the light beam output by the collimating flat-top device to the spot focusing lens; the spot focusing lens is used to receive the excitation light beam The light beam is focused into a point light spot or a line light spot and output to a test card with a fluorescent substance. The fluorescent substance on the surface of the test card is excited by the point light spot or the line light spot to generate a fluorescent signal. The fluorescent signal is collected by the light spot focusing lens to form an emission light beam and then vertically reflected to the emission filter through the excitation / emission beam splitter. The emission filter is used to filter stray light from the light beam reflected by the excitation / emission beam splitter. The emission focusing lens is used to converge the emission light beam output by the emission filter. The photoelectric detection device is used to receive the emission light beam output by the emission focusing lens and convert it into an electrical signal. The direction of the emission light beam received by the photoelectric detection device is consistent with the direction of the excitation light beam emitted by the excitation light source device. The present invention greatly improves the detection signal-to-noise ratio while reducing the relative height of the entire system to half of that of a general module through the innovation of the optical path and the film system structure, and the combination of different film systems and different materials.

[0007] The embodiment of the present application can obtain an excitation light beam of a specific wavelength by setting an excitation light source device in the fluorescence detection system, can block the passage of the excitation light beam at a large angle by setting an aperture device, can achieve angle collimation and beam shaping of the excitation light beam by setting a collimating flat-top device, can separate the excitation light beam and the emission light beam by setting an excitation / emission beam splitter, can focus the excitation light beam into a point light spot or a line light spot by setting a light spot focusing lens to improve the energy density of the excitation light beam on the test card, can collect the emission light beam to improve the energy of the emission signal, can filter the excitation light signal by setting an emission filter to improve the signal-to-noise ratio, can converge the emission light beam and then incident on the photoelectric detection device by setting an emission focusing lens and a photoelectric detection device to achieve efficient photoelectric signal conversion. In this way, the volume of the fluorescence detection system can be compressed while the detection sensitivity is greatly improved.

[0008] Furthermore, the excitation light source device comprises an ultraviolet light source device for emitting an excitation light beam, and the excitation light beam is used to excite the fluorescent signal of the test card.

[0009] In the embodiment of the present application, an excitation light source device is provided to emit the excitation light beam and transmit it along the propagation direction of the optical path. The excitation light beam has a certain divergence angle and a wavelength of 365nm±10nm.

[0010] Furthermore, the excitation light beam emitted by the excitation light source device passes through the aperture device and then enters the collimating flat-top device.

[0011] The embodiment of the present application sets a light-transmitting aperture of the aperture device to block the large-angle excitation light beam from passing through, thereby correcting the angle and spot shape of the excitation light beam. The light-transmitting aperture of the aperture device is 1.5 to 2.5 mm (most preferably 2 mm), and the aperture surface is matte treated.

[0012] Furthermore, the collimating flat-top device comprises a first lens and a second lens sequentially arranged along the emitting direction of the aperture device, the first lens performs preliminary focusing on the excitation light beam, and the second lens performs further collimation on the excitation light beam.

[0013] The embodiment of the present application arranges the collimating flat-top device to change the luminous angle and light intensity distribution of the excitation light beam, so that the Gaussian light energy distribution is as uniform as possible, and the beam energy loss of the excitation light beam during transmission is reduced. The first lens and the second lens are glass spherical surfaces, the material is H-K9L, and the transmittance is 365nm±10nm.

[0014] Furthermore, the excitation / emission beam splitter device includes a blue-reverse-red-transmitting filter group and a red-reverse-blue-transmitting filter group. The blue-reverse-red-transmitting filter group and the red-reverse-blue-transmitting filter group are arranged in an "X" shape through structural positioning, and each filter is set at 90°.

[0015] The embodiment of the present application realizes the separation of the excitation light beam and the emission light beam by setting an excitation / emission beam splitter device. The anti-blue and red-transmitting filter group is coated with an anti-blue-purple and red-transmitting diaphragm film and an anti-reflection film on both sides, which can reflect the excitation light beam and focus it on the test card, and the emission light beam can "losslessly" pass through the anti-blue and red-transmitting filter group; the anti-red and blue-transmitting filter group is coated with an anti-red and blue-purple and diaphragm film and an anti-reflection film on both sides, which can reflect the emission light beam and focus it on the photoelectric detection device, and the excitation light beam can "losslessly" pass through the anti-red and blue-transmitting filter group during transmission. The film system requirements of the blue-reflecting and red-transmitting filter group are: when the incident angle is between 37° and 53°, Tmax<1%@350-400nm, Tmax>96%580-650nm; the film system requirements of the red-reflecting and blue-transmitting filter group are: when the incident angle is between 37° and 53°, Tmax>96%@350-400nm, Tmax<1%@580-650nm.

[0016] Furthermore, the system further comprises: a light spot focusing lens; the light spot focusing lens forms a high energy density light spot on the surface of the test card by focusing the collimated excitation light beam.

[0017] The embodiment of the present application can focus the collimated excitation light beam by setting a spot focusing lens, and finally form a point spot or a line spot on the surface of the test card. The spot focusing lens can be a glass spherical lens or a glass cylindrical lens, and the material is H-K9L.

[0018] Furthermore, the system also includes: an emission filter; the emission filter includes a bandpass film system with a high cut-off depth and an anti-reflection film system, which filters the reflected emission light beam and transmits it to the photoelectric detection device.

[0019] The embodiment of the present application improves the signal-to-noise ratio of the entire detection system by setting an emission filter, which can block most of the excitation light beam and other stray light from passing through, and has a high transmittance for the emission light beam, i.e., the effective fluorescence signal. The emission filter film system requirements are as follows: Tmax>96%@590-630nm, OD>6.

[0020] Furthermore, the system also includes an emission focusing lens; the emission focusing lens focuses the reflected emission light beam and transmits it to the photoelectric detection device.

[0021] The embodiment of the present application sets an emission focusing lens so that the emission light beam is focused and received by the photoelectric detection device. The emission focusing lens is a glass spherical lens made of H-ZLAF92. The emission focusing lens can absorb the excitation light beam and achieve high transmittance for the emission light beam. The transmittance of the excitation light beam is 0.9%, and the internal transmittance of the emission light beam is 98.4%, which further improves the signal-to-noise ratio of the entire system.

[0022] Furthermore, the system also includes a photoelectric detection device for receiving the emission light beam, and the photoelectric detection device is used to convert the received emission light beam into an electrical signal, thereby further amplifying the detection signal.

[0023] Most preferably, the compact fluorescence detection system comprises:

[0024] An excitation light source device, used for emitting an excitation light beam with a specific wavelength;

[0025] Aperture device, used to block the passage of the excitation light beam at a large angle;

[0026] Collimation flat-top device, used for angular collimation and beam shaping of the excitation beam;

[0027] An excitation / emission beam splitter, used to separate an excitation beam and an emission beam;

[0028] A spot focusing lens is used to focus the excitation beam into a point spot or a line spot;

[0029] An emission filter, used to filter the excitation light signal;

[0030] A transmission focusing lens, used for converging the transmission light beam;

[0031] Photoelectric detection device, used to receive the transmitted light beam and convert it into an electrical signal.

[0032] Compared with the prior art, the present invention has the following beneficial technical effects:

[0033] First, the excitation light path uses high-transmittance materials. Through lens shaping and focusing, the energy density of the excitation beam spot at the test card position is improved, greatly increasing the effective energy of the excitation beam;

[0034] Second, the introduction of the "X" arrangement of filter groups innovated the optical path design and reduced the entire vertical height. Compared with the general module, the overall height was reduced by half, providing new ideas for the innovation of flat devices.

[0035] Third, the film system design is optimized for specific bands, making the film layer more uniform and flatter.

[0036] Fourth, the present invention realizes a compact fluorescence detection system with ultra-high cutoff depth through reasonable material selection and coating curve optimization. Combined with the absorption of the material background and the bandpass film system with high cutoff depth (OD>6), the OD value of the entire system is increased to 7-8, greatly improving the detection accuracy and signal-to-noise ratio.

[0037] Other features and advantages of the present application will be described in the following description, and partly become apparent from the description, or be understood by practicing the embodiments of the present application. The purpose and other advantages of the present application can be realized and obtained by the structures specifically pointed out in the written description, claims, and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments of the present application will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0039] Figure 1 A schematic diagram of the structure of a compact fluorescence detection system provided in an embodiment of the present application;

[0040] Figure 2 A schematic diagram of the optical path of a compact fluorescence detection system provided in an embodiment of the present application;

[0041] Figure 3 A schematic diagram of the structure of an excitation / emission beam splitting device provided in an embodiment of the present application;

[0042] Figure 4 A schematic diagram of the structure of a traditional fluorescence detection system provided in an embodiment of the present application.

[0043] Figure 5 A schematic diagram of a design curve of a blue-purple-transmitting red film system for an excitation / emission beam splitting device provided in an embodiment of the present application;

[0044] Figure 6 A schematic diagram of a design curve of a red-reflecting and blue-purple-transmitting film system for an excitation / emission beam splitting device provided in an embodiment of the present application;

[0045] Figure 7 A schematic diagram of a wide-band anti-reflection film system design curve provided in an embodiment of the present application;

[0046] Figure 8 A schematic diagram of a film system design curve of an emission filter provided in an embodiment of the present application;

[0047] Icons: 1-excitation light source device; 2-aperture device; 3-collimation flat top device; 4-excitation / emission beam splitter device; 5-spot focusing lens; 6-test card; 7-emission filter; 8-emission focusing lens; 9-photoelectric detection device. DETAILED DESCRIPTION

[0048] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application.

[0049] In the description of this application, it should be noted that the terms "inside", "outside", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the product of the application is usually placed when in use, which is only for the convenience of describing this application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application. In addition, the terms "first", "second", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0050] Figure 1A structural schematic diagram of a compact fluorescence detection system provided in an embodiment of the present application, the embodiment of the present application provides a compact fluorescence detection system, including: an excitation light source device 1, an aperture device 2, a collimating flat-top device 3, an excitation / emission beam splitter device 4, a spot focusing lens 5, a test card 6, an emission filter 7, an emission focusing lens 8 and a photoelectric detection device 9; the excitation light source device 1 is used to emit an excitation light beam with a specific wavelength and transmit it to the aperture device 2; the aperture device 2 is used to block the passage of an excitation light beam at a large angle; the collimating flat-top device 3 performs angle collimation and beam shaping on the excitation light beam passing through the aperture device 2; the excitation / emission beam splitter device 4 is used to separate the excitation light beam and the emission light beam; the spot focusing lens 5 is used to focus the excitation light beam into a point light spot or a line light spot in the plane of the test card 6; the emission filter 7 is used to filter the excitation light signal and improve the signal-to-noise ratio; the emission focusing lens 8 is used to converge the emission light beam; the photoelectric detection device 9 is used to receive the emission light beam and convert it into an electrical signal.

[0051] First, considering that the traditional fluorescent immunochromatographic detection system is limited by the traditional DM spectroscopic optical path, the overall vertical height is difficult to reduce, and there are problems such as large overall volume and low detection sensitivity. Especially for miniaturized handheld devices, it is difficult to balance size and detection sensitivity.

[0052] In the optional implementation process of the present application, the following are arranged in the fluorescence detection system: an excitation light source device 1, an aperture device 2, a collimating flat-top device 3, an excitation / emission beam splitter device 4, a spot focusing lens 5, a test card 6, an emission filter 7, an emission focusing lens 8 and a photoelectric detection device 9. By combining different spectroscopic film systems in different areas of the excitation / emission beam splitter device 4, the incident direction of the excitation light beam can be kept consistent with the receiving direction of the emission light beam, thereby reducing the longitudinal height of the entire fluorescence detection system. Combined with the high cutoff filtering characteristics of the bandpass film system of the emission filter 7 and the excitation light (365nm) absorption effect of the emission focusing lens 8, a fluorescence detection system with a high signal-to-noise ratio is finally realized.

[0053] Figure 2 A schematic diagram of the optical path of a compact fluorescence detection system provided in an embodiment of the present application is shown in FIG. Figure 2 As shown, the excitation light source device 1 emits an excitation light beam. The excitation light beam emitted by the excitation light source device is usually 365nm and has a certain divergence angle.

[0054] Secondly, the excitation light source device 1 emits an excitation light beam which is transmitted to the collimating flat-top device 3 through the aperture device 2. The aperture device 2 blocks the excitation light beam at a large angle by giving an effective aperture, and only allows the excitation light beam within a specific angle to pass through. The collimating flat-top device 3 performs angle collimation and beam shaping on the excitation light beam passing through the aperture device 2. At the same time, the aperture device 2 and the collimating flat-top device 3 are combined to change the divergence angle and angular energy distribution of the excitation light beam without changing the propagation direction of the light beam, thereby ensuring that the energy loss of the excitation light beam during transmission is reduced to a minimum.

[0055] Furthermore, after the excitation light beam is incident through the excitation / emission beam splitter 4, the excitation / emission beam splitter 4 reflects the excitation light beam and focuses it on the surface of the test card 6 through the light spot focusing lens 5. The excitation / emission beam splitter 4 achieves selective reflection or transmission of a specific wavelength band by coating a spectroscopic film on the glass surface. The light spot focusing lens 5 converges the excitation light beam to form a small light spot on the surface of the test card, thereby increasing the energy density of the excitation light and improving the excitation efficiency.

[0056] Afterwards, the fluorescent material on the surface of the test card 6 is excited by the excitation light beam to generate a fluorescent signal. The fluorescent signal is collected by the spot focusing lens 5 to form an emission light beam that returns along the original light path, and is reflected to the opposite side of the excitation light path by the excitation / emission beam splitter 4. The reflected emission light beam is incident on the emission filter 7. The excitation / emission beam splitter 4 separates the excitation light beam and the emission light beam by coating different spectroscopic films on different glass surfaces. The surface of the emission filter 7 is coated with a bandpass film with a high cut-off depth, which can allow the emission light beam to pass through and block the excitation light beam and other stray light signals, thereby improving the signal-to-noise ratio of the fluorescence detection system.

[0057] Finally, the emission light beam is converged by the emission focusing lens 8 and transmitted to the photoelectric detection device 9, which converts the received emission light beam into an electrical signal for further signal processing. The emission focusing lens 8 is made of a high-refractive index glass substrate, which can absorb the stray light energy of the excitation light beam while converging the emission light beam, further improving the signal-to-noise ratio of the entire detection system.

[0058] The photoelectric detection device 9 is mainly used to convert the emission light beam with the fluorescent signal into an electrical signal. The photoelectric detection device 9 can be a photodiode. The specific type of the photoelectric detection device 9 is not limited and can be adjusted according to actual projection requirements.

[0059] Therefore, through the fluorescence detection system provided in the embodiment of the present application, by combining different splitting film systems in different areas of the excitation / emission beam splitting device 4, the incident direction of the excitation light beam can be kept consistent with the receiving direction of the emission light beam, thereby reducing the longitudinal height of the entire fluorescence detection system. In combination with the high cutoff filtering characteristics of the bandpass film system of the emission filter 7 and the excitation light (365nm) absorption effect of the emission focusing lens 8, a compact fluorescence detection system with a high signal-to-noise ratio is finally realized.

[0060] Based on the above embodiments, Figure 3 As shown, the excitation / emission beam splitter 4 is composed of 4 regional spectroscopic films. The specific type of the excitation / emission beam splitter 4 is not limited, and generally has two configurations: a flat plate "x-plate" or a prism "x-cube". The flat plate "x-plate" beam splitter is composed of 4 flat plate spectroscopes, of which the 02 and 04 surfaces are coated with a spectroscopic film system that reflects blue purple and transmits red, the 06 and 08 surfaces are coated with a spectroscopic film system that reflects red and transmits blue purple, and the remaining 01, 03, 05 and 07 surfaces are coated with a wide-band anti-reflection film system; the prism "x-cube" beam splitter is composed of 4 triangular prisms, of which the 101 and 102 surfaces are coated with a spectroscopic film system that reflects blue purple and transmits red, the 103 and 104 surfaces are coated with a spectroscopic film system that reflects red and transmits blue purple, the remaining 105, 106 and 107 surfaces are coated with a wide-band anti-reflection film, and the 108 surface is subjected to extinction treatment. The requirements for the blue-purple-reflecting and red-transmitting spectral film system, the red-reflecting and blue-purple-transmitting spectral film system, and the wide-band anti-reflection film system are as follows: for the blue-purple-reflecting and red-transmitting spectral film system, when the incident angle is between 37° and 53°, Tmax<1%@350-400nm and Tmax>96%@580-650nm; for the red-reflecting and blue-purple-transmitting spectral film system, when the incident angle is between 37° and 53°, Tmax>96%@350-400nm and Tmax<1%@580-650nm; for the wide-band anti-reflection film system, when the incident angle is between 37° and 53°, Rmax<2%@450-650nm.

[0061] Figure 4 A schematic diagram of the structural comparison between a traditional fluorescence detection system and a compact fluorescence detection system provided in an embodiment of the present application. The traditional fluorescence detection system realizes the splitting of the excitation beam and the emission beam through a DM. The excitation beam and the emission beam are arranged at 90°, and the longitudinal height is difficult to compress. The compact fluorescence detection system innovates the optical path design by setting different splitting film systems on different surfaces, and finally realizes the multiplexing and compression of the optical path, reducing the entire longitudinal height. Compared with the general module, the overall height is reduced by half, which provides a new idea for the innovation of flat equipment.

[0062] According to the requirements of the above coating design indicators, the film system is designed for natural light. As shown in Table 1, the blue-purple-reflecting and red-transmitting splitting film is composed of 31 layers of optical thin films, including two materials with high refractive index Nb2O5 and low refractive index SiO2, which are alternately composed, with a total thickness of 1838.77nm.

[0063] Table 1

[0064]

[0065] As shown in Table 2, the red-reflecting and blue-violet-transmitting splitter film is composed of 60 layers of optical thin films including two materials, high-refractive-index Ta2O5 and low-refractive-index SiO2, alternating with each other, and has a total thickness of 5251.14 nm.

[0066] Table 2

[0067]

[0068] As shown in Table 3, the wide-band anti-reflection film is composed of 5 layers of optical thin films including two materials of high refractive index H4 and low refractive index MgF2, and the total thickness is 276.44nm.

[0069] Table 3

[0070]

[0071] Figure 5 This is a schematic diagram of a blue-purple-transmitting red spectroscopic film curve provided in the example of this application. It has been optimized and adjusted for specific bands (345-385nm, 590-630nm). The actual curve flatness and characteristics within the angle range meet the use requirements and comply with the film design requirements.

[0072] Figure 6 A schematic diagram of a design curve of a red-reflecting blue-purple film system provided in an embodiment of the present application is optimized and adjusted for a specific wavelength band (345-385nm, 590-630nm). The flatness and characteristics of the actual curve within the angle range meet the use requirements and meet the film system design requirements;

[0073] Figure 7 A schematic diagram of a wide-band anti-reflection film system design curve provided in an embodiment of the present application is optimized and adjusted for the band (450-650nm). The actual curve flatness and characteristics within the angle range meet the use requirements and meet the film system design requirements;

[0074] Figure 8A schematic diagram of a film system design curve of an emission filter provided in an embodiment of the present application, wherein the surface of the emission filter is coated with a bandpass film system with a high cut-off depth, which is optimized and adjusted for a specific emission band. The actual curve flatness and characteristics within the angle range meet the use requirements and comply with the film system design requirements. It can allow the emission light beam (590-630nm) to pass through and block the excitation light beam (345-385nm) and other stray light signals, thereby improving the signal-to-noise ratio of the fluorescence detection system.

[0075] In summary, the embodiment of the present application provides a compact fluorescence detection system, including: an excitation light source device 1, an aperture device 2, a collimating flat-top device 3, an excitation / emission beam splitter device 4, a spot focusing lens 5, a test card 6, an emission filter 7, an emission focusing lens 8 and a photoelectric detection device 9; the excitation light source device 1 is used to emit an excitation light beam with a specific wavelength and transmit it to the aperture device 2; the aperture device 2 is used to block the passage of an excitation light beam at a large angle; the collimating flat-top device 3 performs angle collimation and beam shaping on the excitation light beam passing through the aperture device 2; the excitation / emission beam splitter device 4 is used to separate the excitation light beam and the emission light beam; the spot focusing lens 5 is used to focus the excitation light beam into a point light spot or a line light spot in the plane of the test card 6; the emission filter 7 is used to filter the excitation light signal and improve the signal-to-noise ratio; the emission focusing lens 8 is used to converge the emission light beam; the photoelectric detection device 9 is used to receive the emission light beam and convert it into an electrical signal. By combining different spectroscopic film systems in different areas of the excitation / emission beam splitting device 4, the incident direction of the excitation light beam can be kept consistent with the receiving direction of the emission light beam, thereby reducing the longitudinal height of the entire fluorescence detection system. Combined with the high cutoff filtering characteristics of the bandpass film system of the emission filter 7 and the excitation light (365nm) absorption effect of the emission focusing lens 8, a fluorescence detection system with a high signal-to-noise ratio is finally achieved.

[0076] The above description is only an embodiment of the present application and is not intended to limit the protection scope of the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A compact fluorescence detection system comprising: The excitation light source device, the aperture device, the collimating flat-top device, the light spot focusing lens, the emission filter, the emission focusing lens and the photoelectric detection device are characterized in that they also include: an excitation / emission beam splitting device; The excitation light source device is used to emit an excitation light beam; The aperture device is used to limit the aperture of the excitation light beam; The collimating flat-top device is used to collimate and shape the excitation light beam output by the aperture device; The excitation / emission beam splitter is used to vertically reflect the light beam output by the collimating flat-top device to the light spot focusing lens; The emission filter is used to filter stray light from the light beam reflected by the excitation / emission beam splitter; The emission focusing lens is used to converge the emission light beam output by the emission filter; The photoelectric detection device is used to receive the emission light beam output by the emission focusing lens and convert it into an electrical signal.

2. The compact fluorescence detection system according to claim 1, characterized in that: The spot focusing lens is used to focus the received excitation light beam into a point spot or a line spot and output it to a test card with fluorescent material. The fluorescent material on the surface of the test card is excited by the point spot or the line spot to generate a fluorescent signal. The fluorescent signal is collected by the spot focusing lens to form an emission light beam and then vertically reflected to the emission filter through the excitation / emission beam splitter.

3. The compact fluorescence detection system according to claim 1, characterized in that: The excitation / emission beam splitting device comprises two filters coated with blue-purple-reflecting and red-transmitting spectral films and two filters coated with red-reflecting and blue-purple-transmitting spectral films.

4. The compact fluorescence detection system according to claim 3, characterized in that: Two filters coated with blue-violet-reflecting and red-transmitting spectral films and two filters coated with red-reflecting and blue-violet-transmitting spectral films are arranged in an X shape to form a flat plate x-plate; Two filters coated with blue-purple-reflecting and red-transmitting spectral films are on a first straight line, the blue-purple-reflecting and red-transmitting spectral films on the two filters are located on the same side of the first straight line, and an anti-reflection film system is provided on the other side of the first straight line where the two filters are located; Two filters coated with red-reflecting and blue-violet-transmitting spectral films are on the second straight line. The red-reflecting and blue-violet-transmitting spectral films on the two filters are located on the same side of the second straight line. An anti-reflection film system is provided on the other side of the second straight line where the two filters are located.

5. The compact fluorescence detection system according to claim 1, characterized in that: The excitation / emission beam splitter device includes two triangular prisms coated with blue-purple-reflecting and red-transmitting beam splitting films and two triangular prisms coated with red-reflecting and blue-purple-transmitting beam splitting films, and four triangular prisms are spliced ​​to form a prism x-cube; The first plane of the intersecting surface in the prism x-cube is coated with a blue-violet-reflecting and red-transmitting spectral film; The second plane of the intersecting surface in the prism x-cube is coated with a red-reflecting and blue-violet-transmitting dichroic film.

6. The compact fluorescence detection system according to claim 1, characterized in that: The direction of the emission light beam received by the photoelectric detection device is consistent with the direction of the excitation light beam emitted by the excitation light source device.

7. The compact fluorescence detection system according to claim 1, characterized in that: The surface of the aperture device is matte treated and is provided with a light-through hole.

8. The compact fluorescence detection system according to claim 1, characterized in that: The collimating and flattening device comprises a first lens and a second lens which are sequentially arranged along the emission direction of the aperture device; the first lens and the second lens are used to collimate and flatten the light emitted by the light source.

9. The compact fluorescence detection system according to claim 1, characterized in that: The emission filter comprises a bandpass film system with a high cut-off depth and an anti-reflection film system.

10. A compact fluorescence detector for immunochromatography, characterized in that: A compact fluorescence detection system comprising any one of claims 1 to 9 above.

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