Sample detection method, device and system and storage medium

Abstract

The embodiment of the invention provides a sample detection method, device and system and a storage medium, and the sample detection method comprises the following steps: carrying out purification treatment on a sample by using a purification device to obtain a plurality of purified fractions corresponding to the sample; grouping the plurality of purified fractions to obtain at least one group of purified fractions corresponding to the sample; determining a target purified fraction in each group of purified fractions, and conveying the container filled with the target purified fraction to detection equipment through a transfer device; and detecting the target purified fraction by using detection equipment to obtain a detection result of the target purified fraction. According to the embodiment of the invention, the efficiency of the purification and detection experiment process can be improved.

Description

Technical Field

[0001] This application relates to the field of detection technology, and particularly relates to a sample detection method, device, system and storage medium. Background Art

[0002] In scientific experiments, the purification and detection of samples are important processes. The equipment required for sample purification and the equipment required for detection are different, and the interaction between the equipment required for purification and the equipment required for detection all requires manual operation and monitoring. Due to the need for a large amount of manual intervention, the efficiency of the entire purification and detection experimental process is low, increasing the workload and time cost, and it is easy to have errors caused by manual operation. Summary of the Invention

[0003] Embodiments of this application provide a sample detection method, device, system and storage medium, which can improve the efficiency of the purification and detection experimental process.

[0004] In a first aspect of the embodiments of this application, a sample detection method is provided. The method is applied to a sample detection system, and the sample detection system includes: a transfer device, a purification device and a detection device; the method includes:

[0005] Using the purification device to purify the sample to obtain multiple purified fractions corresponding to the sample;

[0006] Grouping the multiple purified fractions to obtain at least one group of purified fractions corresponding to the sample;

[0007] Determining the target purified fraction in each group of purified fractions, and transporting the container containing the target purified fraction to the detection device through the transfer device;

[0008] Using the detection device to detect the target purified fraction to obtain the detection result of the target purified fraction.

[0009] In a second aspect of the embodiments of this application, a sample detection device is provided. The sample detection device is applied to a sample detection system, and the sample detection system includes: a transfer device, a purification device and a detection device; the sample detection device includes:

[0010] A purification processing unit, configured to use the purification device to purify the sample to obtain multiple purified fractions corresponding to the sample;

[0011] A grouping unit, configured to group the multiple purified fractions to obtain at least one group of purified fractions corresponding to the sample;

[0012] A determination unit, configured to determine the target purified fraction in each group of purified fractions;

[0013] A transfer unit, configured to transport a container containing the target purified fraction to the detection device through the transfer device;

[0014] A detection unit, configured to detect the target purified fraction by using the detection device to obtain a detection result of the target purified fraction.

[0015] A third aspect of the embodiments of the present application provides an electronic device, including a processor and a memory. The memory is configured to store a computer program, the computer program includes program instructions, and the processor is configured to call the program instructions to execute the step instructions in the first aspect of the embodiments of the present application.

[0016] A fourth aspect of the embodiments of the present application provides a computer-readable storage medium. The computer-readable storage medium stores a computer program for electronic data exchange. The computer program includes program instructions, and when the program instructions are executed by a processor, the processor is caused to execute the step instructions in the first aspect of the embodiments of the present application.

[0017] A fifth aspect of the embodiments of the present application provides a computer program product. The computer program product includes a computer program, the computer program includes program instructions, and when the program instructions are executed by a processor, the processor is caused to execute the step instructions in the first aspect of the embodiments of the present application.

[0018] A sixth aspect of the embodiments of the present application provides a sample detection system, including: a transfer device, a purification device, a detection device, and a control device. The transfer device, the purification device, and the detection device are all communicatively connected to the control device;

[0019] The purification device is configured to perform a purification process on a sample to obtain a plurality of purified fractions corresponding to the sample;

[0020] The control device is configured to perform a grouping process on the plurality of purified fractions to obtain at least one group of purified fractions corresponding to the sample;

[0021] The control device is further configured to determine a target purified fraction in each group of purified fractions;

[0022] The transfer device is configured to transport a container containing the target purified fraction to the detection device;

[0023] The detection device is configured to detect the target purified fraction to obtain a detection result of the target purified fraction.

[0024] Optionally, the sample detection system further includes a storage device;

[0025] The storage device is used to place a container containing a sample when receiving a feeding instruction;

[0026] The transfer device is further used to transport the container containing the sample to the purification equipment.

[0027] Optionally, the sample detection system further includes a silo;

[0028] The transfer device is further used to transport a container containing the multiple purified fractions to the silo; and / or,

[0029] The transfer device is further used to transport a container containing the target purified fraction to the silo after the detection of the target purified fraction is completed.

[0030] Optionally, the detection equipment includes a sample preparation module and a detection module;

[0031] The sample preparation module is used to prepare a sample from the target purified fraction to obtain a prepared sample;

[0032] The detection module is used to detect the prepared sample to obtain the detection result of the target purified fraction.

[0033] Optionally, the transfer device includes a robotic arm and a ground rail, and the robotic arm is slidably connected to the ground rail; the sample detection system further includes a storage device and a silo, and the purification equipment, the detection equipment, the storage device and the silo are respectively arranged around the ground rail, and the ground rail is used to drive the robotic arm to move between the purification equipment, the detection equipment, the storage device and the silo.

[0034] In the sample detection method of the embodiment of the present application, the purification equipment is used to purify the sample to obtain multiple purified fractions corresponding to the sample; the multiple purified fractions are grouped to obtain at least one group of purified fractions corresponding to the sample; the target purified fraction in each group of purified fractions is determined, and the container containing the target purified fraction is transported to the detection equipment through the transfer device; the detection equipment is used to detect the target purified fraction to obtain the detection result of the target purified fraction. In the embodiment of the present application, the purification equipment can be used to purify the sample, the purified fractions obtained after the purification treatment can be grouped, and the target purified fraction in each group of purified fractions can be transported to the detection equipment through the transfer device for detection. There is no need for manual intervention between purification and detection, and full-automatic interaction is achieved through the transfer device, thereby improving the experimental process efficiency of purification and detection. Description of the Drawings

[0035] To more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0036] Figure 1 is a schematic structural diagram of a sample detection system provided by an embodiment of the present application;

[0037] Figure 2 is a schematic flowchart of a sample detection method provided by an embodiment of the present application;

[0038] Figure 3 is a schematic flowchart of another sample detection method provided by an embodiment of the present application;

[0039] Figure 4 is a schematic specific structural diagram of a sample detection system provided by an embodiment of the present application;

[0040] Figure 5 is a schematic structural diagram of a sample detection device provided by an embodiment of the present application;

[0041] Figure 6 is a schematic structural diagram of an electronic device provided by an embodiment of the present application. Detailed implementation manners

[0042] The following will clearly and completely describe the technical solutions in the embodiments of the present application with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some embodiments of the present application, rather than all embodiments. Based on the embodiments of the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present application.

[0043] The terms "first", "second", etc. in the specification and claims of the present application and the above drawings are used to distinguish different objects, rather than to describe a specific order. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product or device that includes a series of steps or units is not limited to the listed steps or units, but optionally further includes steps or units not listed, or optionally further includes other steps or units inherent to these processes, methods, products or devices.

[0044] References to "embodiments" in this application mean that the specific features, structures, or characteristics described in connection with the embodiments can be included in at least one embodiment of this application. The phrase appears in various places in the specification and does not necessarily refer to the same embodiment each time, nor are they independent or alternative embodiments mutually exclusive of other embodiments. Those skilled in the art will explicitly and implicitly understand that the embodiments described in this application can be combined with other embodiments.

[0045] The electronic device involved in the embodiments of this application is a device with data processing and computing capabilities. It can be a mobile phone, a tablet computer, a laptop computer, an ultra-mobile personal computer (UMPC), a netbook, a digital signal processor, a microcontroller unit (MCU), etc.

[0046] In the embodiments of this application, a purification device can be used to purify a sample. The purified fractions obtained after the purification process can be grouped. The target purified fraction in each group of purified fractions can be transported to a detection device by a transfer device for detection. There is no need for manual intervention between purification and detection, and full-automatic interaction is achieved through the transfer device, thereby improving the efficiency of the experimental processes of purification and detection. The following will be specifically described.

[0047] Please refer to Figure 1 , Figure 1 which is a schematic structural diagram of a sample detection system provided by the embodiments of this application. As Figure 1 shown, the sample detection system can include: a transfer device, a purification device, a detection device, and a control device (not shown in the figure); the transfer device, the purification device, and the detection device are all communicatively connected to the control device;

[0048] The purification device is used to purify a sample to obtain various purified fractions corresponding to the sample;

[0049] The control device is used to group various purified fractions to obtain at least one group of purified fractions corresponding to the sample;

[0050] The control device is further used to determine the target purified fraction in each group of purified fractions;

[0051] The transfer device is used to transport the container containing the target purified fraction to the detection device;

[0052] The detection device is used to detect the target purified fraction to obtain the detection result of the target purified fraction.

[0053] In the embodiments of the present application, the purification equipment may include a column chromatography machine, which can be used to purify a sample by the column chromatography machine to obtain a purified fraction corresponding to the sample. The purification equipment may adopt Flash technology for purification. Flash technology is a technology commonly used for separating and purifying compounds, and is applicable to the fields of organic synthesis and drug discovery.

[0054] The transfer device can provide logistics connection capabilities and transfer materials between the purification equipment and the detection equipment. Exemplarily, the transfer device can be any one of a robot and an automated guided vehicle (AGV). The robot may include a floor-mounted robot, which is a robot moving on a floor-mounted track.

[0055] The detection equipment may include a liquid chromatography-mass spectrometry (LCMS) instrument. The target purified fraction can be detected by the LCMS instrument to obtain the detection result of the target purified fraction. LCMS is an analytical technique that combines liquid chromatography (LC) and mass spectrometry (MS) and is used in the fields of chemical and biological analysis. It can be understood that the detection equipment may also include an MS instrument, which is not limited here.

[0056] In the embodiments of the present application, the purification equipment purifies the sample, and the purified fractions obtained after the purification treatment can be grouped. The target purified fraction in each group of purified fractions can be transported to the detection equipment by the transfer device for detection. There is no need for manual intervention between purification and detection, and full-automatic interaction is realized through the transfer device, thereby improving the efficiency of the experimental process of purification and detection.

[0057] Optionally, Figure 1 It may further include a storage device.

[0058] The storage device is used to place a container containing a sample when receiving a feeding instruction;

[0059] The transfer device is further used to transport the container containing the sample to the purification equipment.

[0060] The user can place the container containing the sample in the storage device. The transfer device is further used to transport the container containing the sample to the purification equipment.

[0061] In the embodiments of the present application, the user can send a feeding instruction to the storage device, and then the storage device can automatically open after receiving the feeding instruction. After the storage device is opened, the user or an external mobile robot can place the container containing the sample in the storage device.

[0062] Optionally, Figure 1 it may further include a silo.

[0063] The transfer device is further configured to transport the container filled with the multiple purified fractions to the silo.

[0064] The transfer device can provide logistics connection capabilities and can transfer materials between the storage device, the purification equipment, the detection equipment, and the silo.

[0065] The transfer device includes a robotic arm and a ground rail, and the robotic arm is slidably connected to the ground rail; the purification equipment, the detection equipment, the storage device, and the silo are respectively arranged around the ground rail, and the ground rail is used to drive the robotic arm to move between the purification equipment, the detection equipment, the storage device, and the silo.

[0066] The transfer device is configured to grab the container filled with the target purified fraction through the robotic arm, and drive the robotic arm through the ground rail to transport the container filled with the target purified fraction to the detection equipment. The transfer device can grab the container filled with the target purified fraction from the purification equipment or the silo through the robotic arm.

[0067] Exemplarily, the transfer device may include a robotic arm and a ground rail logistics system. The ground rail logistics system may include multiple ground rails, and the storage device, the purification equipment, the detection equipment, and the silo can be connected through the multiple ground rails. By using the robotic arm and the ground rail logistics system, automatic connection between devices is achieved, improving the experimental efficiency and accuracy. The robotic arm and the ground rail can accurately complete the sampling and transfer of containers (such as test tubes), reducing the error of manual operation.

[0068] Optionally, after the detection of the target purified fraction is completed, the transfer device is further configured to separately transport the container filled with the target purified fraction to the silo; and / or,

[0069] The transfer device is further configured to transport the container filled with the multiple purified fractions to the silo.

[0070] The storage device is further configured to, when receiving the discharging instruction for the sample, control the transfer device to transport the container filled with the purified fraction corresponding to the sample from the silo to the storage device, and discharge the container filled with the purified fraction corresponding to the sample.

[0071] In the embodiment of the present application, after the detection of the target purified fraction is completed, the container of the target purified fraction can be transported to the silo through a transfer device. When the user needs to discharge the material, the user can send a discharge instruction for the sample to the storage device, and control the transfer device to transport the container containing the purified fraction corresponding to the sample from the silo to the storage device, and discharge the container containing the purified fraction corresponding to the sample.

[0072] In the embodiment of the present application, the user only needs to place the container containing the sample in the storage device. Through this sample detection system, the sample can be automatically purified and detected. After the detection is completed, a discharge instruction for the sample is sent to the silo, so that the silo discharges the container containing the purified fraction corresponding to the sample. There is no need for manual intervention between purification and detection, and full-automatic interaction is realized through the transfer device, thereby improving the efficiency of the experimental process of purification and detection.

[0073] Optionally, the detection device includes a sample preparation module and a detection module;

[0074] The sample preparation module is used to prepare a sample from the target purified fraction to obtain a prepared sample;

[0075] The detection module is used to detect the prepared sample to obtain the detection result of the target purified fraction.

[0076] The sample preparation module prepares a sample from the target purified fraction to obtain a prepared sample, which may be to perform dilution treatment and filtration treatment on the target purified fraction to obtain a prepared sample.

[0077] The detection module may be an LCMS instrument or an MS instrument. Optionally, the detection device may further include a robotic arm to realize the material interaction between the detection module and the sample preparation module. It can be understood that the material transfer between the detection module and the sample preparation module can also be realized through a transfer device, which is not limited here.

[0078] Please refer to Figure 2 , Figure 2 which is a schematic flowchart of a sample detection method provided by an embodiment of the present application. Figure 2 The method can be applied to Figure 1 the sample detection system shown. As Figure 2 shown, the method may include the following steps.

[0079] 201, the electronic device uses a purification device to purify a sample to obtain multiple purified fractions corresponding to the sample.

[0080] In the embodiments of the present application, the sample can be directly placed into the purification device manually or transported to the purification device through a transfer device. The sample can be a solution sample and can be placed in a container. The container can be any one of a test tube, a beaker, and a graduated cylinder. The electronic device can be the above-mentioned control device.

[0081] Exemplarily, when the purification device purifies the sample, the sample can be placed under different temperatures and different pressures for distillation to obtain multiple purified fractions.

[0082] Optionally, step 201 can specifically include the following steps:

[0083] The electronic device replaces the corresponding purification column for the sample, and uses the purification column to purify the sample to obtain multiple purified fractions.

[0084] The purification device can include a purification column. A purification column is an experimental tool for biomolecule purification. It can separate target molecules from a mixed sample through the affinity difference of substances on a solid-phase material. The core of the purification column is the solid-phase material, which has a specific affinity for binding to the target molecule. For example, a protein A purification column uses an affinity resin containing protein A and can selectively capture protein A and remove other proteins. When the sample passes through the column, the solid-phase material adsorbs the target molecule, while the impurities flow through the column. Then, the target molecule can be released from the column through an elution step for further analysis or application. Purification columns can be widely used in the biomedical field, including the purification and separation of biomolecules such as proteins and nucleic acids. The purified biomolecules can be used in fields such as structural research, function verification, drug screening, food, and environmental monitoring.

[0085] The purification device can perform ultraviolet spectrophotometric detection on the substances in the purified fractions to obtain spectral information of the purified fractions. Among them, the spectral information can be used to indicate relevant information about the ultraviolet and visible spectra of the purified fractions.

[0086] In a possible embodiment, a robotic arm can be used to replace the corresponding purification column for the sample. The robotic arm can be controlled to install the purification column at a designated position, and then docking the corresponding pipelines can complete the replacement of the purification column. In the embodiments of the present application, through an automated process, the robotic arm is controlled to automatically replace the column as needed to ensure the continuity and efficiency of the experiment.

[0087] In a possible embodiment, the purification column can be replaced manually. The corresponding purification column for the sample can be replaced through manual installation.

[0088] Among them, the purification column can be replaced when one sample has passed through the column. The replacement of N purification columns is supported, and the purification of N samples can be completed at one time. Ensure that the purification equipment can operate continuously for a long time. N can be an integer greater than or equal to 2.

[0089] 202. The electronic device performs grouping processing on multiple purification fractions to obtain at least one group of purification fractions corresponding to the sample.

[0090] In the embodiments of the present application, the multiple purification fractions can be located in the purification equipment or in the storage bin.

[0091] In a possible embodiment, the electronic device can perform grouping processing on multiple purification fractions in the purification equipment to obtain at least one group of purification fractions corresponding to the sample.

[0092] In another possible embodiment, the container containing the multiple purification fractions can be transported to the storage bin through the transfer device. The electronic device can perform grouping processing on the multiple purification fractions in the storage bin to obtain at least one group of purification fractions corresponding to the sample.

[0093] The electronic device can perform grouping processing on multiple purification fractions according to the spectrum information to obtain at least one group of purification fractions corresponding to the sample. The spectrum information of each purification fraction in the same group of purification fractions is close. The same group of purification fractions refers to a group of purification fractions with close spectrum information. The spectrum information can include at least one of ultraviolet absorbance and the slope of the absorption curve.

[0094] Optionally, step 202 can specifically include any one of the following steps (11) to step (13).

[0095] (11) The electronic device performs grouping processing on the multiple purification fractions according to the ultraviolet absorbance to obtain at least one group of purification fractions corresponding to the sample. Each purification fraction in each group of purification fractions is in the same ultraviolet absorbance interval, that is, the ultraviolet absorbances corresponding to all the purification fractions included in the same group of purification fractions are in the same ultraviolet absorbance interval;

[0096] (12) The electronic device performs grouping processing on the multiple purification fractions according to the slope of the absorption curve to obtain at least one group of purification fractions corresponding to the sample. Each purification fraction in each group of purification fractions is in the same slope interval, that is, the slopes of the absorption curves corresponding to all the purification fractions included in the same group of purification fractions are in the same slope interval;

[0097] (13) The electronic device groups the multiple purified fractions according to the ultraviolet absorbance and the slope of the absorption curve, and obtains at least one group of purified fractions corresponding to the sample. Each purified fraction in each group of purified fractions is in the same ultraviolet absorbance range, and each purified fraction in each group of purified fractions is in the same slope range.

[0098] In the embodiments of the present application, when the spectral information includes the ultraviolet absorbance, the spectral information being close can be a group of purified fractions with the ultraviolet absorbance in the same ultraviolet absorbance range. Exemplarily, a group of purified fractions with the ultraviolet absorbance in the ultraviolet absorbance range of 1 - 1.1 can be grouped into the same group of purified fractions.

[0099] When the spectral information includes the slope of the absorption curve, the spectral information being close can be a group of purified fractions with the slope of the absorption curve in the same slope range. Exemplarily, a group of purified fractions with the slope of the absorption curve in the slope range of 1.5 - 2 can be grouped into the same group of purified fractions.

[0100] When the spectral information includes the ultraviolet absorbance and the slope of the absorption curve, the spectral information being close can be a group of purified fractions with the ultraviolet absorbance in the same ultraviolet absorbance range and the slope of the absorption curve in the same slope range. Exemplarily, a group of purified fractions with the ultraviolet absorbance in the ultraviolet absorbance range of 1 - 1.1 and the slope of the absorption curve in the slope range of 1.5 - 2 can be grouped into the same group of purified fractions.

[0101] 203. The electronic device determines the target purified fraction in each group of purified fractions, and transports the container containing the target purified fraction to the detection device through the transfer device.

[0102] In the embodiments of the present application, the same group of purified fractions refers to a group of purified fractions with close spectral information. The spectral information can include at least one of the ultraviolet absorbance and the slope of the absorption curve.

[0103] Optionally, the electronic device determines the target purified fraction in each group of purified fractions, including:

[0104] The electronic device determines the purified fraction with the highest ultraviolet absorbance in each group of purified fractions as the target purified fraction in this group of purified fractions. For example, multiple purified fractions obtained by purifying a sample are grouped to obtain 4 groups of purified fractions. For each group of these purified fractions, the purified fraction with the highest ultraviolet absorbance is obtained as the target purified fraction in this group of purified fractions, thereby obtaining 4 target purified fractions.

[0105] In the embodiments of the present application, the higher the ultraviolet absorbance of the purified fraction, the greater the concentration of the light-absorbing substance in the purified fraction. The substances in the purified fraction can be detected by ultraviolet spectrophotometry using the purification equipment in step 201 to obtain the spectral information of the purified fraction. This spectrum can be an ultraviolet-visible absorption spectrum. A high absorbance of the ultraviolet-visible absorption spectrum indicates that there are more absorbing substances in the sample. Among the same group of purified fractions, the purified fraction with the highest ultraviolet absorbance has a better purification effect. To accurately measure the substance content of the purified fraction with a better purification effect, the purified fraction with the highest ultraviolet absorbance in the same group of purified fractions is selected as the target purified fraction in this group.

[0106] In the embodiments of the present application, after obtaining the purification result (purified fraction) corresponding to the sample, according to the inherent logic (the purified fraction with the highest ultraviolet absorbance in the same group of purified fractions is the target purified fraction in this group), the test tube (the container containing the target purified fraction) that needs to be detected is judged, and the robotic arm and the ground rail system are notified to perform corresponding operations. This can reduce the influence of human factors on the experimental results and improve the consistency and reliability of the experiment.

[0107] 204. The electronic device uses the detection device to detect the target purified fraction to obtain the detection result of the target purified fraction.

[0108] In the embodiments of the present application, the detection device may include an LCMS instrument. The target purified fraction can be detected by the LCMS instrument to obtain the detection result of the target purified fraction.

[0109] Optionally, step 204 may specifically include the following steps:

[0110] The electronic device uses the detection device to prepare a sample of the target purified fraction to obtain a prepared sample, and detects the prepared sample to obtain the detection result of the target purified fraction.

[0111] In the embodiments of the present application, preparing a sample of the target purified fraction to obtain a prepared sample may be to perform dilution treatment and filtration treatment on the target purified fraction to obtain a prepared sample.

[0112] Dilution treatment: The amount of the target purified fraction is relatively large, while only a small amount of liquid is required for detection. In the experiment, usually, the sample with a high absorbance is diluted before measurement to avoid measurement errors caused by too high absorbance. To ensure the accuracy of the subsequent measurement results, generally, the purified fraction with the highest ultraviolet absorbance in the same group of purified fractions is selected as the target purified fraction, and the target purified fraction is diluted before detection.

[0113] Filtration treatment: Filtration is to ensure that the undissolved substances in the diluent are filtered out, ensure the clarity of the prepared sample, and thus improve the reliability of the detection result of the prepared sample.

[0114] In the embodiments of the present application, a purification device can be used to purify a sample. The purified fractions obtained after the purification process can be grouped. The target purified fraction in each group of purified fractions can be transported to a detection device by a transfer device for detection. There is no need for manual intervention between purification and detection, and full-automatic interaction is achieved through the transfer device, thereby improving the efficiency of the experimental processes of purification and detection.

[0115] Please refer to Figure 3 , Figure 3 which is a schematic flowchart of another sample detection method provided by the embodiments of the present application. Figure 3 The method of Figure 1 can be applied to the sample detection system shown in Figure 3 . As shown in

[0116] 301. When receiving a feeding instruction, the electronic device places the container containing the sample in the storage device.

[0117] In the embodiments of the present application, the user can send a feeding instruction to the storage device. After receiving the feeding instruction, the storage device can be automatically opened. After the storage device is opened, the user or an external mobile robot can place the container containing the sample in the storage device.

[0118] Exemplarily, the user can scan the QR code on the storage device through a device such as a mobile phone to send a feeding instruction to the storage device.

[0119] 302. The electronic device transports the container containing the sample to the purification device through the transfer device.

[0120] The transfer device can include a robotic arm and a ground rail system. The container containing the sample (such as a test tube) is transported to the purification device through the robotic arm and the ground rail system.

[0121] 303. The electronic device uses the purification device to purify the sample to obtain various purified fractions corresponding to the sample.

[0122] 304. The electronic device transports the container containing the purified fractions to the silo through the transfer device.

[0123] 305. The electronic device performs grouping processing on the various purified fractions to obtain at least one group of purified fractions corresponding to the sample.

[0124] After performing step 303, the electronic device transports the container filled with the purified fraction to the silo through a transfer device. After obtaining the purified fraction in step 303, one purified fraction is correspondingly filled into one container, and multiple containers filled with different purified fractions are transported to the silo through the transfer device. The container can be any one of a test tube, a beaker, and a measuring cylinder. Due to the limited space of the purification equipment, when there are more purified fractions obtained after the sample is purified, the obtained purified fractions can be transferred by the transfer device to the silo for storage. After the sample purification is completed, all the obtained purified fractions are grouped, so as to ensure the smooth collection of the purified fractions and the accuracy of grouping.

[0125] 306. The electronic device determines the target purified fraction in each group of purified fractions in the silo, and transports the container filled with the target purified fraction to the detection device through the transfer device.

[0126] 307. The electronic device uses the detection device to detect the target purified fraction and obtains the detection result of the target purified fraction.

[0127] Among them, for the specific implementation of steps 303 to 304, reference can be made to the above steps 201 to 202, and for the specific implementation of steps 306 to 307, reference can be made to the above steps 203 to 204, which will not be elaborated here.

[0128] 308. After the detection of the target purified fraction is completed, the electronic device discharges the container filled with the target purified fraction through the transfer device.

[0129] In the embodiment of the present application, the transfer device may include a robotic arm and a ground rail system, and the container (such as a test tube) filled with the target purified fraction is transported to the storage device or the silo through the robotic arm and the ground rail system. In a possible example, after the container (such as a test tube) filled with the target purified fraction is transported to the storage device through the robotic arm and the ground rail system, the user can open the storage device for discharging. Or the user can scan the QR code on the storage device through a device such as a mobile phone, so as to send a discharging instruction for the sample to the storage device, and then discharge.

[0130] In another possible example, after the container (such as a test tube) filled with the target purified fraction is transported to the silo through the robotic arm and the ground rail system, when receiving the discharging instruction for the sample, the electronic device discharges the container filled with the purified fraction corresponding to the sample.

[0131] In the embodiment of the present application, after the detection of the target purified fraction is completed, the container of the target purified fraction can be transported to the silo through the transfer device. The user can send a discharging instruction for the sample to the storage device, control the transfer device to transfer the purified fraction corresponding to the sample from the silo to the storage device, so that the storage device discharges the container filled with the purified fraction corresponding to the sample.

[0132] Exemplarily, the user can scan the QR code on the storage device through a device such as a mobile phone, so as to send a discharging instruction for the sample to the storage device.

[0133] Optionally, after step 307 is executed, step 309 can also be executed.

[0134] 309. The electronic device outputs the detection result of the target purified fraction, and the detection result includes mass spectrometry and purity.

[0135] In the embodiments of the present application, the detection result will be provided to the user in the form of a report. The report can be an electronic report or a paper report. For example, the report can be a report in PDF format.

[0136] In the embodiments of the present application, the container containing the sample can be placed in the storage device, and the container containing the sample can be transported to the purification device through the transfer device. The sample is purified by the purification device, and the purified fraction obtained after the purification process can be transported to the silo through the transfer device. The target purified fraction in the silo can be transported to the detection device through the transfer device for detection. After the detection of the target purified fraction is completed, the container containing the target purified fraction is transported to the silo through the transfer device, the container containing the purified fraction corresponding to the sample is discharged, and the detection result of the target purified fraction can also be output. During the feeding and discharging processes, including purification, detection, and the operation of materials, no manual intervention is required, and full-automatic interaction is realized through the transfer device, thereby improving the efficiency of the experimental process of purification and detection. While improving the experimental efficiency, accuracy, and reliability, functions such as automatic detection after purification are realized, providing convenient and high-quality experimental services for actual users.

[0137] Please refer to Figure 4 , Figure 4 which is a schematic structural diagram of a sample detection system provided by the embodiments of the present application. As Figure 4 shown, the sample detection system may include: a storage rack, a ground rail robot, a Flash medium and low pressure separation and purification workstation, an LCMS detection station, and a silo.

[0138] The storage rack (regarded as the above-mentioned storage device) provides the user with the ability to place the material consumables in the middle process of the experiment (such as sample solutions, tip heads, filter heads, etc.);

[0139] The ground rail robot (regarded as the above-mentioned transfer device) provides the experimental island with logistics connection capabilities and can transfer materials between stations;

[0140] In the Flash medium and low pressure separation and purification workstation (regarded as the above-mentioned purification device), a robotic arm is used to complete operations such as column replacement, and at the same time, the purification process is automatically started according to the instructions issued by the system;

[0141] The silo provides users with the ability to store and manage compounds. The fractions after separation and purification as well as those after detection will be transported by the ground rail and robotic arm to the silo for storage, ensuring safety. Adding a silo to manage the fraction compounds reduces the difficulty for users to manage compounds, reduces error proneness, and at the same time ensures the safety of chemicals and reduces risks.

[0142] The LCMS detection station (regarded as the above-mentioned detection equipment) consists of a dilution and filtration station and an ultraperformance liquid chromatography (UPLC) attachment station, providing users with the ability to prepare samples by dilution and filtration. After the samples to be sent for inspection are prepared, the robotic arm in the station sends them into the LCMS instrument for detection, and the mass spectrum and purity of the samples are fed back to the users.

[0143] Based on Figure 4 the sample detection system shown, the embodiments of the present application provide a sample detection method, which may specifically include the following steps (410) to step (421).

[0144] (410) Manually scan the code for feeding;

[0145] Among them, manual code scanning for feeding can be carried out on the storage rack. After scanning the code, the operator puts the materials into the storage rack manually.

[0146] (411) The ground rail robot transfers materials;

[0147] It is possible to control the ground rail robot to transfer materials when it is detected that there is code-scanned feeding on the storage rack.

[0148] (412) Automatically start the separation and purification instrument;

[0149] The Flash medium and low pressure separation and purification workstation may include a separation and purification instrument. Starting the separation and purification instrument can start the automated purification process. It is possible to automatically start the separation and purification instrument after it is detected that materials are transferred to the Flash medium and low pressure separation and purification workstation.

[0150] (413) The ground rail robot transfers the purified fraction to the silo;

[0151] It is possible to control the ground rail robot to transfer the purified fraction to the silo after it is detected that the Flash medium and low pressure separation and purification workstation has completed the separation and purification of a sample.

[0152] (414) Automatically judge the purification result;

[0153] The purification result can be automatically judged through an electronic device.

[0154] After the purification is completed at the Flash medium and low pressure separation and purification station, spectral information of the purified fractions and fraction grouping information will be obtained. The electronic device will automatically analyze and identify the test tube with the largest value of ultraviolet absorbance in each grouped fraction according to the corresponding information, and issue an instruction to let the ground rail system and the robotic arm transfer the corresponding test tube to the LCMS detection station for sample preparation and testing.

[0155] (415) The ground rail robot transfers the test tube of the fraction to be detected to the LCMS;

[0156] After judging the purification result in step (414), the ground rail robot can be controlled to transfer the test tube of the fraction to be detected to the LCMS;

[0157] (416) The LCMS detection station performs sample preparation and testing on the test tube to be detected;

[0158] After detecting that the test tube of the fraction to be detected is transferred to the LCMS detection station, the LCMS detection station can be controlled to perform sample preparation and testing on the test tube to be detected.

[0159] (417) The ground rail robot transfers the test tube of the fraction with the detection completed to the silo;

[0160] After detecting that the LCMS detection station has completed the detection of the test tube, the ground rail robot can be controlled to transfer the test tube of the fraction with the detection completed to the silo.

[0161] (418) Fraction management and storage;

[0162] Fraction management and storage can be carried out in the silo.

[0163] (419) Manual selection of discharging;

[0164] (420) Transfer the fraction to the storage rack;

[0165] After detecting the manual selection of discharging, the ground rail robot is controlled to transfer the fraction from the silo to the storage rack.

[0166] (421) Manual scanning code for discharging.

[0167] Among them, manual scanning code for feeding can be carried out on the storage rack. After scanning the code, the fraction in the storage rack will be automatically discharged.

[0168] Traditional purification equipment can only perform single-sample purification and requires frequent human intervention. In the embodiments of the present application, a robotic arm is used to replace samples and purification columns, increasing the throughput of the purification part from traditional single-sample to continuous purification of multiple samples, significantly improving human efficiency. After purification, the samples are automatically detected, which can greatly improve the working efficiency of users and directly obtain experimental results. While considering efficiency, a feed bin is added to manage fractionated compounds, reducing the difficulty for users to manage compounds, reducing error-proneness, and ensuring the safety of chemicals and reducing risks.

[0169] The above mainly introduces the solutions of the embodiments of the present application from the perspective of the execution process on the method side. It can be understood that in order for an electronic device to implement the above functions, it includes corresponding hardware structures and / or software modules for executing each function. Those skilled in the art should easily realize that, combined with the units and algorithm steps of each example described in the embodiments provided herein, the present application can be implemented in the form of hardware or a combination of hardware and computer software. Whether a certain function is executed in the form of hardware or computer software driving hardware depends on the specific application and design constraints of the technical solution. Professional technicians can use different methods to implement the described functions for each specific application, but such implementation should not be considered to exceed the scope of the present application.

[0170] The embodiments of the present application can divide the functional units of the electronic device according to the above method examples. For example, each functional unit can be divided corresponding to each function, or two or more functions can be integrated into one processing unit. The above integrated units can be implemented in the form of hardware or in the form of software functional units. It should be noted that the division of units in the embodiments of the present application is illustrative, only a logical function division, and there may be other division methods in actual implementation.

[0171] Please refer to Figure 5 , Figure 5 which is a schematic structural diagram of a sample detection device provided by the embodiments of the present application. The sample detection device is applied to a sample detection system, and the sample detection system includes: a transfer device, a purification device, and a detection device; as Figure 5 shown, the sample detection device 500 includes:

[0172] A purification processing unit 501, configured to use the purification device to perform purification processing on a sample to obtain multiple purified fractions corresponding to the sample;

[0173] A grouping unit 502, configured to perform grouping processing on multiple purified fractions to obtain at least one group of purified fractions corresponding to the sample;

[0174] A determination unit 503, configured to determine the target purified fraction in each group of purified fractions;

[0175] A transfer unit 504, configured to transport a container containing the target purified fraction to the detection device through the transfer device;

[0176] A detection unit 505, configured to detect the target purified fraction by using the detection device to obtain a detection result of the target purified fraction.

[0177] Optionally, the sample detection system further includes a storage device;

[0178] The transfer unit 504 is further configured to, upon receiving a feeding instruction, place a container containing a sample in the storage device and then transport the container containing the sample to the purification device through the transfer device.

[0179] Optionally, the sample detection system further includes a silo;

[0180] The transfer unit 504 is further configured to transport a container containing the multiple purified fractions to the silo through the transfer device.

[0181] Optionally, the sample detection device 500 further includes a discharging unit 506.

[0182] The discharging unit 506 is further configured to discharge a container containing the target purified fraction through the transfer device after the detection of the target purified fraction is completed.

[0183] Optionally, the sample detection device 500 further includes an output unit 507.

[0184] The output unit 507 is configured to output the detection result of the target purified fraction after the detection unit 505 uses the detection device to detect the target purified fraction to obtain the detection result of the target purified fraction, and the detection result includes a mass spectrum and purity.

[0185] Optionally, the detection unit 505 uses the detection device to detect the target purified fraction to obtain the detection result of the target purified fraction, including: preparing a sample of the target purified fraction by using the detection device to obtain a prepared sample, and detecting the prepared sample to obtain the detection result of the target purified fraction.

[0186] Optionally, the purification processing unit 501 uses the purification device to perform purification processing on the sample to obtain a purified fraction, including: replacing a corresponding purification column for the sample, and using the purification column to perform purification processing on the sample to obtain a purified fraction.

[0187] Optionally, the determining unit 503 determines the target purified fraction in each group of purified fractions, including: determining the purified fraction with the highest ultraviolet absorbance in each group of purified fractions as the target purified fraction in this group of purified fractions.

[0188] Optionally, the grouping unit 502 performs grouping processing on a plurality of the purified fractions to obtain at least one group of purified fractions corresponding to the sample, including: performing grouping processing on the plurality of purified fractions according to the ultraviolet absorbance to obtain at least one group of purified fractions corresponding to the sample, and each purified fraction in each group of purified fractions is in the same ultraviolet absorbance range; or, performing grouping processing on the plurality of purified fractions according to the slope of the absorption curve to obtain at least one group of purified fractions corresponding to the sample, and each purified fraction in each group of purified fractions is in the same slope range; or, performing grouping processing on the plurality of purified fractions according to the ultraviolet absorbance and the slope of the absorption curve to obtain at least one group of purified fractions corresponding to the sample, and each purified fraction in each group of purified fractions is in the same ultraviolet absorbance range, and each purified fraction in each group of purified fractions is in the same slope range.

[0189] Among them, the purification processing unit 501, the grouping unit 502, the transfer unit 504, the determining unit 503, the detection unit 505, the discharging unit 506, and the output unit 507 may be processors in an electronic device.

[0190] Figure 5 For the specific implementation of the sample detection device 500 shown, reference may be made to Figure 2 and Figure 3 the method embodiments shown, which will not be elaborated here.

[0191] In the embodiments of the present application, a purification device can be used to perform purification processing on a sample. The purified fractions obtained after the purification processing can be grouped. The target purified fraction in each group of purified fractions can be transported to a detection device for detection by a transfer device. There is no need for manual intervention between purification and detection, and full-automatic interaction is realized through the transfer device, thereby improving the experimental process efficiency of purification and detection.

[0192] Please refer to Figure 6 , Figure 6 which is a schematic structural diagram of an electronic device provided by an embodiment of the present application. As shown in Figure 6As shown, the electronic device 600 includes a processor 601 and a memory 602. The processor 601 and the memory 602 can be interconnected via a communication bus 603. The communication bus 603 can be a Peripheral Component Interconnect (PCI) bus or an Extended Industry Standard Architecture (EISA) bus, etc. The communication bus 603 can be divided into an address bus, a data bus, a control bus, etc. For ease of representation, Figure 6 only a thick line is shown in [the figure], but it does not mean that there is only one bus or one type of bus. The memory 602 is used to store a computer program. The computer program includes program instructions. The processor 601 is configured to call the program instructions. The above program includes those for executing Figure 2 and Figure 3 some or all of the steps in the method shown.

[0193] The memory 602 can be a Read-Only Memory (ROM) or other type of static storage device that can store static information and instructions, a Random Access Memory (RAM) or other type of dynamic storage device that can store information and instructions, or it can also be an Electrically Erasable Programmable Read-Only Memory (EEPROM), a Compact Disc Read-Only Memory (CD-ROM) or other optical disc storage, optical disc storage (including compact discs, laser discs, optical discs, digital versatile discs, Blu-ray discs, etc.), magnetic disk storage media or other magnetic storage devices, or any other medium that can be used to carry or store the desired program code in the form of instructions or data structures and can be accessed by a computer, but is not limited thereto. The memory can exist independently and be connected to the processor via a bus. The memory can also be integrated with the processor.

[0194] In the embodiments of the present application, a purification device can be used to purify a sample. The purified fractions obtained after the purification treatment can be grouped. The target purified fraction in each group of purified fractions can be transported to a detection device for detection by a transfer device. There is no need for manual intervention between purification and detection, and full-automatic interaction is achieved through the transfer device, thereby improving the experimental process efficiency of purification and detection.

[0195] The embodiments of the present application also provide a computer-readable storage medium. The computer-readable storage medium stores a computer program for electronic data exchange, and the computer program enables a computer to execute some or all of the steps of any one of the sample detection methods described in the foregoing method embodiments.

[0196] It should be noted that, for the foregoing method embodiments, for the sake of simple description, they are all expressed as a series of action combinations. However, those skilled in the art should know that the present application is not limited by the described action sequence, because according to the present application, some steps can be performed in other sequences or simultaneously. Secondly, those skilled in the art should also know that the embodiments described in the specification are all preferred embodiments, and the actions and modules involved are not necessarily essential to the present application.

[0197] In the above embodiments, the descriptions of the respective embodiments have their own emphases. For the parts not detailed in a certain embodiment, reference may be made to the relevant descriptions of other embodiments.

[0198] In several embodiments provided by the present application, it should be understood that the disclosed device can be implemented in other ways. For example, the device embodiments described above are merely illustrative. For example, the division of the units is only a logical function division. In actual implementation, there may be other division methods. For example, multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the displayed or discussed coupling or direct coupling or communication connection between each other can be through some interfaces. The indirect coupling or communication connection of the device or unit can be in an electrical or other form.

[0199] The unit described as a separated component may or may not be physically separated, and the component displayed as a unit may or may not be a physical unit, that is, it may be located in one place, or may be distributed to multiple network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0200] In addition, each functional unit in the various embodiments of the application can be integrated in a processing unit, or each unit can exist physically alone, or two or more units can be integrated in one unit. The above integrated unit can be implemented in the form of hardware or in the form of a software program module.

[0201] When the integrated unit is implemented in the form of a software program module and sold or used as an independent product, it can be stored in a computer-readable memory. Based on this understanding, the technical solution of the present application, in essence, or the part that contributes to the prior art, or all or part of the technical solution, can be embodied in the form of a software product. The computer software product is stored in a memory and includes several instructions for causing a computer device (which can be a personal computer, an electronic device, or a network device, etc.) to execute all or part of the steps of the methods described in the various embodiments of the present application. The aforementioned memory includes various media that can store program codes, such as USB flash drives, read-only memories (ROMs), random access memories (RAMs), mobile hard disks, magnetic disks, or optical discs.

[0202] Those of ordinary skill in the art can understand that all or part of the steps in the various methods of the above embodiments can be completed by instructing relevant hardware through a program. The program can be stored in a computer-readable memory, and the memory can include: flash drives, read-only memories, random access memories, magnetic disks, or optical discs, etc.

[0203] The above has introduced the embodiments of the present application in detail. Specific examples are used in this article to elaborate on the principle and implementation manner of the present application. The description of the above embodiments is only used to help understand the method and its core idea of the present application; at the same time, for those of ordinary skill in the art, according to the idea of the present application, there will be changes in the specific implementation manner and application scope. In summary, the content of this specification should not be construed as a limitation to the present application.

Claims

1. A sample detection method, characterized in that: The method is applied to a sample detection system, which includes: a transport device, a purification device and a detection device; the method includes: Purifying the sample using the purification device to obtain multiple purified fractions corresponding to the sample; performing grouping processing on the plurality of the purified fractions to obtain at least one group of purified fractions corresponding to the sample; determining a target purified fraction in each group of purified fractions, and transporting a container containing the target purified fraction to the detection device through the transport device; The target purified fraction is detected by using the detection device to obtain a detection result of the target purified fraction.

2. The method according to claim 1, characterized in that The sample detection system further includes a storage device. Before the purification device is used to purify the sample to obtain a plurality of purified fractions corresponding to the sample, the method further includes: Upon receiving a feeding instruction, placing a container containing a sample in the storage device; The container containing the sample is transported to the purification equipment by the transport device.

3. The method according to claim 1, characterized in that The sample detection system further includes a silo. After obtaining the plurality of purified fractions corresponding to the sample, the method further includes: The containers containing the plurality of purified fractions are transported to the silo by the transfer device.

4. The method according to claim 1, characterized in that: After the detection device is used to detect the target purified fraction and the detection result of the target purified fraction is obtained, the method further comprises: After the detection of the target purified fraction is completed, the container containing the target purified fraction is discharged through the transfer device.

5. The method according to claim 1, characterized in that After the detection device is used to detect the target purified fraction and the detection result of the target purified fraction is obtained, the method further comprises: The detection result of the target purified fraction is output, wherein the detection result includes a mass spectrum and purity.

6. The method according to any one of claims 1 to 5, characterized in that: The detecting device is used to detect the target purified fraction to obtain the detection result of the target purified fraction, including: The target purified fraction is sampled using the detection device to obtain a sample preparation sample, and the sample preparation sample is tested to obtain a test result of the target purified fraction.

7. The method according to any one of claims 1 to 5, characterized in that: The step of determining a target purified fraction in each group of purified fractions comprises: The purified fraction with the highest ultraviolet absorbance in each group of purified fractions is determined as the target purified fraction in the group of purified fractions.

8. The method according to any one of claims 1 to 5, characterized in that: The grouping of the plurality of purified fractions to obtain at least one group of purified fractions corresponding to the sample comprises: The plurality of purified fractions are grouped according to ultraviolet absorbance to obtain at least one group of purified fractions corresponding to the sample, wherein each purified fraction in each group of purified fractions is located in the same ultraviolet absorbance interval; Alternatively, the plurality of purified fractions are grouped according to the slope of the absorption curve to obtain at least one group of purified fractions corresponding to the sample, wherein each purified fraction in each group of purified fractions is located in the same slope interval; Alternatively, the plurality of purified fractions are grouped according to the ultraviolet absorbance and the slope of the absorption curve to obtain at least one group of purified fractions corresponding to the sample, wherein each purified fraction in each group of purified fractions is located in the same ultraviolet absorbance interval and the same slope interval.

9. A sample detection device, characterized in that: The sample detection device is applied to a sample detection system, and the sample detection system comprises: a transport device, a purification device and a detection device; the sample detection device comprises: A purification processing unit, used to purify the sample using the purification device to obtain a plurality of purified fractions corresponding to the sample; A grouping unit, used for grouping the plurality of purified fractions to obtain at least one group of purified fractions corresponding to the sample; a determination unit, configured to determine a target purified fraction in each group of purified fractions; A transport unit, used for transporting the container containing the target purified fraction to the detection equipment through the transport device; The detection unit is used to detect the target purified fraction using the detection device to obtain the detection result of the target purified fraction.

10. An electronic device, characterized in that: The method comprises a processor and a memory, wherein the memory is used to store a computer program, the computer program comprises program instructions, and the processor is configured to call the program instructions to execute the method according to any one of claims 1 to 8.

11. A computer-readable storage medium, characterized in that: The computer-readable storage medium stores a computer program, wherein the computer program includes program instructions, and when the program instructions are executed by a processor, the processor is caused to execute the method according to any one of claims 1 to 8.

12. A sample detection system, characterized in that: include: A transport device, a purification device, a detection device and a control device, wherein the transport device, the purification device and the detection device are all in communication connection with the control device; The purification device is used to purify the sample to obtain a plurality of purified fractions corresponding to the sample; The control device is used to group the plurality of purified fractions to obtain at least one group of purified fractions corresponding to the sample; The control device is further used to determine a target purified fraction in each group of purified fractions; The transport device is used to transport the container containing the target purified fraction to the detection equipment; The detection device is used to detect the target purified fraction to obtain the detection result of the target purified fraction.

13. The system according to claim 12, characterized in that The sample detection system also includes a storage device; The storage device is used to place the container containing the sample when receiving the feeding instruction; The transport device is also used to transport the container containing the sample to the purification equipment.

14. The system according to claim 12, characterized in that The sample detection system also includes a silo; The transfer device is further used to transport the containers containing the plurality of purified fractions to the silo; and / or, The transfer device is also used to transport the container containing the target purified fraction to the silo after the detection of the target purified fraction is completed.

15. The system according to any one of claims 12 to 14, characterized in that: The detection equipment comprises a sample preparation module and a detection module; The sample preparation module is used to prepare the target purified fraction to obtain a prepared sample; The detection module is used to detect the prepared sample to obtain the detection result of the target purified fraction.

16. The system according to any one of claims 12 to 14, characterized in that: The transfer device includes a robotic arm and a ground rail, and the robotic arm is slidably connected to the ground rail; the sample detection system also includes a storage device and a silo, and the purification equipment, the detection equipment, the storage device and the silo are respectively arranged around the ground rail, and the ground rail is used to drive the robotic arm to move between the purification equipment, the detection equipment, the storage device and the silo.

Images