A composition, preparation and use of a combination of a plant essential oil and benzyl isothiocyanate

Abstract

The application discloses a plant essential oil and benzyl isothiocyanate composition, preparation and application, effective components are active ingredient A and active ingredient B, the active ingredient A is benzyl isothiocyanate, and the active ingredient B is selected from one or more of lemon grass oil, eugenol, catnip oil, verbena oil and peppermint oil.The weight ratio of the active ingredient A to the active ingredient B in the composition is 1:20-20:1.The compound agent can be prepared into oil agent, water emulsion, soluble liquid agent, microemulsion, granules and the like, and is applied to the prevention and treatment of soil nematodes, soil pests and soil-borne pathogenic bacteria.The compound agent has obvious synergistic effect, high bacteriostatic and nematocidal activity, and less relative dosage, and has obvious fumigation effect on soil nematodes, soil-borne pathogenic fungi and other harmful organisms.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pesticide combination, and relates to a plant source component composition for preventing and treating harmful organisms (nematodes, soil-borne pathogenic fungi, soil-borne pathogenic bacteria and soil pests) in soil and application thereof, in particular, a composition containing plant essential oil and benzyl isothiocyanate, a preparation and application. BACKGROUND

[0002] Plant parasitic nematodes are widely distributed and have a large variety of species in the world, and are one of the main pathogenic organisms that harm agricultural production. According to statistics, there are more than 5000 species of plant parasitic nematodes in the world, causing losses of more than 173 billion US dollars. Among them, the most serious impact on crops is a class of root-knot nematodes. It is found that the damage of root-knot nematodes is widespread in various food crops, economic crops, horticultural seedlings, etc., more than 3000 species. Among them, the most serious yield reduction is caused by cruciferous vegetables, solanaceous vegetables, leguminous vegetables and liliaceous vegetables. In addition to directly causing crop death, root-knot nematodes can also synergize with other soil-borne diseases to cause complex infections and aggravate diseases. At the same time, the wounds caused by nematodes when invading plants also open the door for the invasion of bacteria, fungi and viruses, greatly affecting the quality and yield of agricultural products and seriously threatening the sustainable development of agriculture.

[0003] The prevention and control methods of root-knot nematodes include chemical control, physical control, agricultural control, genetic engineering breeding and biological control, among which chemical control is the main measure. Traditional chemical control methods rely mainly on fumigating chemical pesticides, among which high-toxicity pesticides dominate. Such pesticides have high cost, strong toxicity and great harm to the environment. With the improvement of public environmental awareness and the increase of safety standards for agricultural products, high-toxicity pesticides have been gradually banned and eliminated. At present, the types of prevention and control agents for plant nematode diseases on the market are limited, mainly including abamectin, fostic-acid, fluopyram and fluensulfone. Especially, fostic-acid and abamectin and their compound preparations have been the market mainstream for a long time. However, the effect of fostic-acid is poor at low dosage, and high dosage is easy to damage roots; abamectin has poor horizontal migration in soil, is easily adsorbed by soil particles or degraded by microorganisms, resulting in fluctuation and instability of the control effect.

[0004] Plant resources in nature are extremely rich, and are an important source of natural products. Compared with traditional chemical pesticides, the use of plant extracts to prevent and control pests and diseases has many advantages. It has lower toxicity, is easy to decompose, can coexist with the environment in harmony, and is not easy to cause resistance, which is of great benefit to the healthy growth of plants and biological safety protection. At present, the most widely used plant source insecticides in the world include nicotine, aldehyde compounds, ketone compounds, sulfur-containing compounds, triterpenoids, alkaloids, glycosides, flavonoids and various plant essential oils.

[0005] Benzyl isothiocyanate, also known as benzyl isothiocyanate, benzyl mustard oil, etc., is a light yellow oily liquid, which is widely present in plants of Brassicaceae, especially Brassica and Eutrema plants. In addition to the flavoring effect, people have widely studied the various biological activities of isothiocyanate compounds, and it has been proved that isothiocyanate has the ability to kill nematodes or inhibit bacteria, such as southern root-knot nematode, pine nematode, salmonella, staphylococcus aureus, tuberculosis, salmonella, diphtheria, escherichia coli, bacillus subtilis, etc. have strong inhibitory activity. Fungi such as Paecilomyces variotii and beer yeast also have high inhibition rate. As a potential nematode-killing bactericide, benzyl isothiocyanate has certain research value and application prospect.

[0006] How to utilize plant source pesticides under the premise of higher biological safety to improve the plant nematode control effect for farmers is a problem to be solved at present. SUMMARY

[0007] The purpose of the present application is to provide a composition of benzyl isothiocyanate and plant essential oil with obvious synergistic effect and a complex agent.

[0008] Another purpose of the present application is to provide the application of benzyl isothiocyanate and plant essential oil complex agent in preparing a pesticide fumigant for preventing and treating soil-borne pathogens and soil nematodes.

[0009] In order to achieve the above purpose, the technical scheme adopted by the present application is:

[0010] A composition containing plant essential oil and benzyl isothiocyanate, the effective components of the composition are active ingredient A and active ingredient B;

[0011] The active ingredient A is benzyl isothiocyanate;

[0012] The active ingredient B is selected from at least one of lemon grass oil, eugenol, catnip oil, verbena oil and peppermint oil;

[0013] The weight ratio of active ingredient A and active ingredient B is (1-20):(20-1).

[0014] Optionally, the composition comprises: the weight ratio of benzyl isothiocyanate and lemon grass oil is 1:20-15:1; preferably 1:1;

[0015] The weight ratio of benzyl isothiocyanate and eugenol is 13:1-1:9; preferably 1:5;

[0016] The weight ratio of benzyl isothiocyanate and catnip oil is 9:1-1:9; preferably 1:5;

[0017] The weight ratio of benzyl isothiocyanate and verbena oil is 15:1-1:9; preferably 1:3;

[0018] The weight ratio of benzyl isothiocyanate to peppermint oil is 20:1 to 1:15; preferably 5:1.

[0019] A preparation of a plant essential oil and benzyl isothiocyanate composition, the preparation containing any of the plant essential oil and benzyl isothiocyanate compositions described herein;

[0020] The active ingredient A is 1% to 15% and the active ingredient B is 1% to 30% by mass percentage in the preparation.

[0021] Optionally, the preparation is an oil agent, and the components and mass percentage contents are as follows:

[0022] The active ingredient A is 1% to 15%, the active ingredient B is 1% to 30%, the antioxidant is 0.1% to 2%, the solvent is 10% to 85%, and the plant oil is supplemented to 100%.

[0023] The antioxidant is ascorbic acid or pentagalloyl resorcinol; the solvent is at least one of ethyl formate, sec-butyl acetate, furfural, 5-hydroxymethyl furfural, 5-methyl furfural, or ethyl acetate; and the plant oil is turpentine oil or camphor oil.

[0024] Optionally, the preparation is an emulsion in water, and the components and mass percentage contents are as follows:

[0025] The active ingredient A is 1% to 10%, the active ingredient B is 1% to 35%, the emulsifier is 12% to 28%, the ascorbic acid is 0.1% to 2%, the solvent is 10% to 30%, and water is supplemented to 100%.

[0026] The emulsifier is Farm Emulsion 500, 1601, Tween-80, TX-10, OP-10, or Span 80; and the solvent is at least one of ethyl formate, sec-butyl acetate, furfural, 5-hydroxymethyl furfural, 5-methyl furfural, or ethyl acetate.

[0027] Optionally, the preparation is a soluble liquid agent, and the components and mass percentage contents are as follows:

[0028] The active ingredient A is 1% to 20%, the active ingredient B is 1% to 30%, the emulsifier is 12% to 60%, the ascorbic acid is 1% to 8%, and the solvent is supplemented to 100%.

[0029] The emulsifier is Farm Emulsion 500, 1601, Tween-80, TX-10, OP-10, or Span 80; and the solvent is at least one of ethyl formate, sec-butyl acetate, furfural, 5-hydroxymethyl furfural, 5-methyl furfural, or ethyl acetate.

[0030] Optionally, the preparation is a microemulsion, and the components and mass percentage contents are as follows:

[0031] active ingredient A 1-15%, active ingredient B 1-30%, solvent 10-40%, emulsifier 5-20%, ascorbic acid 0.1-2%;

[0032] The emulsifier is at least one of Farm Emulsion 500, 1601, Tween-80, TX-10, OP-10 or Span 80; and the solvent is at least one of ethyl formate, sec-butyl acetate, furfural, 5-hydroxymethyl furfural, 5-methyl furfural or ethyl acetate.

[0033] Optionally, the preparation is a granule, and the components and the weight percentage contents are as follows:

[0034] active ingredient A 1-15%, active ingredient B 1-30%, solvent 10-40%, emulsifier 5-20%, ascorbic acid 0.1-2%;

[0035] The carrier filler is at least one of starch, bentonite, diatomite and light calcium carbonate; the disintegrant is at least one of cellulose ether and its derivatives, sodium carboxymethyl starch and bentonite; the binder is at least one of sodium carboxymethyl cellulose, xanthan gum and polyethylene glycol; and the auxiliary agent is at least one of dextrose, calcium lignosulfonate and magnesium aluminum silicate.

[0036] The application of the composition containing plant essential oil and benzyl isothiocyanate or the preparation of the composition containing plant essential oil and benzyl isothiocyanate according to any of the application to prepare a fumigant for controlling soil-borne pathogens and / or soil nematodes.

[0037] Optionally, the soil-borne pathogens include Agrobacterium tumefaciens and / or Fusarium oxysporum f. sp. niveum;

[0038] The soil nematode is Meloidogyne hapla.

[0039] The application has the following advantages:

[0040] The compound agent of the application has obvious synergistic effect, high bacteriostatic and nematocidal activity, and less relative amount, and has obvious fumigation effect on soil nematodes, soil-borne pathogenic fungi and other harmful organisms. DETAILED DESCRIPTION

[0041] The composition containing plant essential oil and benzyl isothiocyanate of the application has the following features: active ingredient A is benzyl isothiocyanate, active ingredient B is at least one of lemon grass oil, eugenol, catnip oil, verbena oil and mint oil, and the weight ratio of active ingredient A to active ingredient B is 1:20-20:1.

[0042] The preferred combination is: the weight ratio of benzyl isothiocyanate to lemon grass oil is 1:20-20:1; the weight ratio of benzyl isothiocyanate to eugenol is 1:9-13:1; the weight ratio of benzyl isothiocyanate to catmint oil is 1:9-9:1; the weight ratio of benzyl isothiocyanate to verbena oil is 1:9-15:1; and the weight ratio of benzyl isothiocyanate to peppermint oil is 1:15-20:1.

[0043] The plant essential oil and benzyl isothiocyanate composition of the present application is used as a preparation.

[0044] The preparation is an oil agent, and the components and weight percentage contents are: active ingredient A 1%-15%, active ingredient B 1%-30%, antioxidant 0.1%-2%, solvent 10%-85%, and plant oil to 100%; wherein the antioxidant is ascorbic acid or pentagalloyl resorcinol; the solvent is one or several of ethyl formate, sec-butyl acetate, furfural, 5-hydroxymethyl furfural, 5-methyl furfural, or ethyl acetate; and the plant oil is turpentine or camphor oil. The preparation process is: first, the antioxidant is put into the mixed mother liquor of the benzyl isothiocyanate and the plant extracted essential oil, and then the plant oil and the solvent are mixed uniformly. Finally, the two solutions are mixed in a preparation tank and continuously stirred for 1-3 hours. The product is packed in double barrier plastic bottles or aluminum bottles with an inner barrier layer, and stored in a sealed manner.

[0045] The preparation is a water emulsion, and the components and weight percentage contents are: active ingredient A 1%-10%, active ingredient B 1%-35%, emulsifier 12%-28%, ascorbic acid 0.1%-2%, solvent 10%-30%, and water to 100%; wherein the emulsifier is nongyou 500, 1601, Tween-80, TX-10, OP-10, or span 80; and the solvent is one or several of ethyl formate, sec-butyl acetate, furfural, 5-hydroxymethyl furfural, 5-methyl furfural, or ethyl acetate. The preparation process is: first, the ascorbic acid is put into the mixed mother liquor of the benzyl isothiocyanate and the plant extracted essential oil, and then the emulsifier and the solvent are mixed uniformly. Finally, the two solutions are continuously stirred in a preparation tank for 1-3 hours. The product is packed in double barrier plastic bottles, glass bottles, or aluminum bottles with an inner barrier layer, and stored in a sealed manner.

[0046] The preparation is a soluble liquid agent, and the components and weight percentage contents are as follows: active ingredient A 1%-20%, active ingredient B 1%-30%, emulsifier 12%-60%, ascorbic acid 1%-8%, and solvent supplementing to 100%; wherein the emulsifier is Farm Emulsion 500, 1601, Tween-80, TX-10, OP-10 or Span 80; and the solvent is one or several of ethyl formate, sec-butyl alcohol acetate, furfural, 5-hydroxymethyl furfural, 5-methyl furfural or ethyl acetate. The preparation process is as follows: firstly, the ascorbic acid is put into the mixed mother liquor of the benzyl isothiocyanate and the plant extraction essential oil which are weighed accurately, and then mixed uniformly. The agricultural emulsifier and the solvent which are weighed accurately are mixed uniformly. Finally, the two solutions are continuously stirred in the preparation tank for 1-3 hours. The product is divided into double barrier plastic bottles for storage.

[0047] The preparation is a microemulsion, and the components and weight percentage contents are as follows: active ingredient A 1%-15%, active ingredient B 1%-30%, solvent 10%-40%, emulsifier 5%-20%, and ascorbic acid 0.1%-2%; wherein the emulsifier is Farm Emulsion 500, 1601, Tween-80, TX-10, OP-10 or Span 80; and the solvent is one or several of ethyl formate, sec-butyl alcohol acetate, furfural, 5-hydroxymethyl furfural, 5-methyl furfural or ethyl acetate. The preparation process is as follows: the benzyl isothiocyanate and the plant extraction essential oil are mixed uniformly in the preparation tank (1). The ascorbic acid is put into the preparation tank (1) and mixed uniformly. The emulsifier is added into the preparation tank (2), and then the solvent is added after mixing and stirred uniformly. The materials in the preparation tank (2) are moved into the preparation tank (1) through the pipeline, and then the tank opening is sealed and the stirrer is started. The product is divided into double barrier plastic bottles, and each bottle contains 500 mL of the medicine.

[0048] The preparation is a granule, and the components and weight percentage contents are as follows: active ingredient A 1%-10%, active ingredient B 1%-30%, carrier filler 60%-80%, disintegrating agent 1%-3%, binder 1%-3%, and auxiliary agent 0%-10%; the carrier filler is selected from the group consisting of starch, kaolin, diatomite and light calcium carbonate; the disintegrating agent includes cellulose ether and its derivatives, sodium carboxymethyl starch and bentonite; the binder includes sodium carboxymethyl cellulose, xanthan gum and polyethylene glycol (PEG); and the auxiliary agent includes glucose, calcium lignosulfonate and magnesium aluminum silicate. One or more of the above is selected. The preparation process is as follows: the granule is prepared by the extrusion granulation method. Firstly, a certain amount of benzyl isothiocyanate and plant essential oil are mixed with the carrier filler for adsorption, and then the binder, the disintegrating agent and the auxiliary agent are added and mixed uniformly. Then, the extrusion granulator is used for granulation, and the prepared granule is dried in a drying oven at 60°C for 6 hours. The qualified granule is packaged with suitable packaging materials, such as aluminum foil bags or plastic bottles.

[0049] The above production processes are completed by machines under closed conditions, and workers should avoid direct contact with the liquid medicine. The production workers should have perfect protection measures.

[0050] The plant source component composition of the present application is used for preparing a fumigant for controlling soil-borne pathogens and / or soil nematodes. The plant source component composition preparation of the present application is used for preparing a fumigant for controlling soil-borne pathogens and / or soil nematodes.

[0051] The Fusarium oxysporum and Agrobacterium tumefaciens used in the following experiments are conventional pathogens, which can be obtained by laboratory self-isolation and identification, or purchased on the market.

[0052] Experimental Example 1: Indoor fumigation activity determination of five plant essential oils, benzyl isothiocyanate and their compound agents on root-knot nematodes

[0053] The indoor fumigation activity determination of five plant essential oils, benzyl isothiocyanate and their compound agents on root-knot nematodes is carried out by using an indoor desiccator fumigation method. After fumigation in the desiccator, the number of nematode deaths in the twenty-four-hole plate is counted under a microscope with a bottom light source at 24 h, 48 h and 72 h, and the number of nematodes in each field of view is about 200. The death of nematodes is observed and recorded.

[0054] The purchased five plant essential oils, benzyl isothiocyanate and their compound agents are prepared into different concentrations with acetone for preliminary screening of the fumigation activity on root-knot nematodes. A total of 10 treatments are set for the test:

[0055] (1) 5 μg / mL lemon grass oil;

[0056] (2) 5 μg / mL eugenol;

[0057] (3) 5 μg / mL catmint oil;

[0058] (4) 5 μg / mL verbena oil;

[0059] (5) 5 μg / mL peppermint oil;

[0060] (6) 5 μg / mL benzyl isothiocyanate

[0061] (7) 5 μg / mL lemon grass oil + 5 μg / mL benzyl isothiocyanate (1:1);

[0062] (8) 5 μg / mL eugenol + 5 μg / mL benzyl isothiocyanate (1:1);

[0063] (9) 5 μg / mL catmint oil + 5 μg / mL benzyl isothiocyanate (1:1);

[0064] (10) 5 μg / mL Lippia oil + 5 μg / mL benzyl isothiocyanate (1:1);

[0065] (11) 5 μg / mL Peppermint oil + 5 μg / mL benzyl isothiocyanate (1:1);

[0066] Table 1 Fumigant activity of different plant essential oils and their combinations against Meloidogyne incognita

[0067]

[0068]

[0069] Lemon grass oil, eugenol, catmint oil, lippia oil and peppermint oil all had certain fumigant activity against M. incognita at a concentration of 5 μg / mL, among which lemon grass oil had the strongest fumigant activity, with a mortality rate of 99.1% against M. incognita after 72 h of fumigation. After being combined with benzyl isothiocyanate, all of them showed synergistic effects, and the bioactivity of the combinations was significantly higher than that of the single agents, which could significantly reduce the use amount of benzyl isothiocyanate.

[0070] Experimental Example 2: Determination of the indoor contact activity of plant essential oils, benzyl isothiocyanate and their combinations against M. incognita, with a total of 11 treatment groups:

[0071] (1) 25 μg / mL Lemon grass oil;

[0072] (2) 25 μg / mL Eugenol;

[0073] (3) 25 μg / mL Catmint oil;

[0074] (4) 25 μg / mL Lippia oil;

[0075] (5) 25 μg / mL Peppermint oil;

[0076] (6) 25 μg / mL Benzyl isothiocyanate;

[0077] (7) 25 μg / mL Lemon grass oil + 25 μg / mL Benzyl isothiocyanate (1:1);

[0078] (8) 25 μg / mL Eugenol + 25 μg / mL Benzyl isothiocyanate (1:1);

[0079] (9) 25 μg / mL Catmint oil + 25 μg / mL Benzyl isothiocyanate (1:1);

[0080] (10) 25 μg / mL Lippia oil + 25 μg / mL Benzyl isothiocyanate (1:1);

[0081] (11) 25 μg / mL of peppermint oil + 25 μg / mL of benzyl isothiocyanate (1:1);

[0082] Table 2 In vitro contact toxicity of different plant essential oils and their combinations against Meloidogyne incognita

[0083]

[0084]

[0085] The test was carried out by soaking the nematodes in the liquid. The second instar larvae were added to the suspension containing the plant essential oils at a certain concentration in a 24-well plate. The mortality of the nematodes was observed and counted after 24 h and 48 h. The preliminary screening results showed that the plant essential oils of lemon grass, eugenol, catnip, verbena and peppermint had certain contact toxicity against the root-knot nematodes at a concentration of 25 μg / mL. The contact toxicity of the lemon grass oil was the best, with a corrected mortality of 70.21% after 48 h. The benzyl isothiocyanate had a 24 h and 48 h control effect of 57.21% and 69.10% against the root-knot nematodes at a concentration of 25 μg / mL. The bioactivity of the combination was obviously higher than that of the single agent, indicating that the combination had a synergistic effect.

[0086] Example 3 In vitro fumigation toxicity of five plant essential oils and benzyl isothiocyanate against Meloidogyne incognita

[0087] The fumigation toxicity of the five plant essential oils and benzyl isothiocyanate against the root-knot nematodes was determined by the method of Example 1. The results are shown in Table 3.

[0088] Table 3 Analysis of the fumigation activity of the five agents against Meloidogyne incognita (48 h)

[0089]

[0090] As shown in Table 3, the LC50 of the five plant essential oils and benzyl isothiocyanate against the root-knot nematodes were at a low level, indicating that they had high fumigation activity. The LC50 of the lemon grass oil was the lowest, only 1.25 μl / L, followed by the benzyl isothiocyanate, 2.25 μl / L. 50 50

[0091] Example 4 Determination of the joint toxicity of the combination of lemon grass oil and benzyl isothiocyanate against Meloidogyne incognita

[0092] The co-toxicity coefficient (CTC) was calculated by the method of Sun Yunpei, and the synergistic effect of the combination of the agents was qualitatively evaluated according to the co-toxicity coefficient. The LC50 of the optimal combination of the agents was determined, and one of the single agents was set as a standard nematocide. The LC50 of the combination was compared with that of the single agent to determine the synergistic effect. 50 50 ​​​The ratio of the toxicity index of the mixture to the toxicity index of the single agent determines the toxicity index of the mixture. Finally, the CTC is calculated by the actual toxicity index of the mixture and the theoretical toxicity index of the mixture, and the formula is as follows:

[0093]

[0094] Theoretical toxicity index of the mixture = ∑ toxicity index of the single agent × mass percentage of the single agent in the mixture

[0095]

[0096] Note: When CTC≥120, it is synergistic effect; when CTC≤80, it is antagonistic effect; when 80<CTC<120, it is additive effect.

[0097] Table 4 Fumigant toxicity test results of the mixture of lemon grass oil and benzyl isothiocyanate on root-knot nematode

[0098]

[0099] Note: In the table, "N" represents lemon grass oil, and "Y" represents benzyl isothiocyanate.

[0100] The results of Table 4 show that the mixture composed of lemon grass oil and benzyl isothiocyanate has a CTC greater than 120 in the combination of the weight ratio of 1:20 to 15:1, indicating that the two components exhibit synergistic effect in the range of 1:20 to 20:1. When the mass ratio of lemon grass oil to benzyl isothiocyanate is 1:1, the synergistic coefficient is 236.34, and the synergistic effect is the most obvious.

[0101] Experimental Example 5: Fumigant toxicity test results of the mixture of eugenol and benzyl isothiocyanate on root-knot nematode

[0102] Table 5 Fumigant toxicity test results of the mixture of eugenol and benzyl isothiocyanate on root-knot nematode

[0103]

[0104] Note: In the table, "D" represents eugenol, and "Y" represents benzyl isothiocyanate.

[0105] As shown in the results of Table 5, the mixture composed of eugenol and benzyl isothiocyanate has a CTC greater than 120 in the combination of the weight ratio of 1:9 to 13:1, indicating that the two components exhibit synergistic effect in the range of 1:9 to 13:1. When the mass ratio of eugenol to benzyl isothiocyanate is 5:1, the synergistic coefficient is 240.03, and the synergistic effect is the most obvious.

[0106] Experimental Example 6: Fumigant toxicity test results of the mixture of catmint oil and benzyl isothiocyanate on root-knot nematode

[0107] Table 6 Results of fumigation toxicity determination of the compound preparation of catmint oil and benzyl isothiocyanate on root-knot nematode

[0108]

[0109]

[0110] Note: "J" in the table represents catmint oil, and "Y" represents benzyl isothiocyanate.

[0111] As shown in the results of Table 6, the compound preparation composed of catmint oil and benzyl isothiocyanate has a CTC greater than 120 in the combination of the weight ratio of 1:9 to 9:1, indicating that both of them show synergistic effect in the range of 1:9 to 9:1. When the mass ratio of catmint oil to benzyl isothiocyanate is 5:1, the synergistic coefficient is 221.57, and the synergistic effect is the most obvious.

[0112] Experimental Example 7: Determination of the indoor combined toxicity of Verbena oil and benzyl isothiocyanate composition on Agrobacterium tumefaciens

[0113] The indoor biological activity of Verbena oil and benzyl isothiocyanate composition on soil-borne pathogenic bacteria Agrobacterium tumefaciens was determined by the inhibition zone method, and the synergistic effect of the compound preparation was qualitatively evaluated according to the CTC.

[0114] Table 7 Results of indoor biological activity toxicity determination of the compound preparation of Verbena oil and benzyl isothiocyanate on fungi in soil

[0115]

[0116]

[0117] Note: "M" in the table represents Verbena oil, and "Y" represents benzyl isothiocyanate.

[0118] As shown in the results of Table 7, the compound preparation composed of Verbena oil and benzyl isothiocyanate has a CTC greater than 120 in the combination of the weight ratio of 1:9 to 15:1, indicating that both of them show synergistic effect in the range of 1:9 to 15:1. When the mass ratio of Verbena oil to benzyl isothiocyanate is 3:1, the synergistic coefficient is 151.26, and the synergistic effect is the most obvious.

[0119] Experimental Example 8: Determination of the indoor combined toxicity of peppermint oil and benzyl isothiocyanate composition on Fusarium oxysporum

[0120] The indoor biological activity of Verbena oil and benzyl isothiocyanate composition on soil-borne pathogenic fungi Fusarium oxysporum was determined by the inhibition zone method, and the synergistic effect of the compound preparation was qualitatively evaluated according to the CTC.

[0121] Table 8 Results of indoor bioactivity virulence determination of the compound preparation of peppermint oil and benzyl isothiocyanate on bacteria in soil

[0122]

[0123] Note: "B" in the table represents peppermint oil, and "Y" represents benzyl isothiocyanate.

[0124] As shown in the results of Table 8, the compound preparation consisting of peppermint oil and benzyl isothiocyanate has a CTC greater than 120 in the combination of the weight ratio of 1:15-20:1, indicating that both of them show a synergistic effect when mixed in the range of 1:15-20:1. When the mass ratio of peppermint oil to benzyl isothiocyanate is 1:5, the synergistic coefficient is 145.10, and the synergistic effect is most obvious.

[0125] Experimental Example 9: Pot experiment of 5 kinds of plant essential oils, benzyl isothiocyanate and compound preparation on root-knot nematode

[0126] The soil of the plough layer (depth about 0-15 cm) collected from the tomato field with serious root-knot nematode disease in Dali County, Shaanxi Province was incubated and moisturized, and the diseased roots of tomato infected with root-knot nematode were buried in the soil to rot in the soil. After a period of time, the soil contained a large number of tomato root-knot nematode larvae. Then the above soil (absolute water content about 20%) was mixed uniformly and filled into pots, 1 kg per pot. Then, the compound preparation was diluted with water and stirred according to the following concentration gradient: 50, 200 μL / kg (effective ingredient and soil weight ratio), and then the soil was treated. Finally, the pots treated with the drug and sand were sealed with polyvinyl chloride plastic film for 7 days, and then opened for 4 days, and then transplanted tomato seedlings cultivated for 14 days. At the same time, 20% horseradish water emulsion with a concentration of 50 mg / kg was used as a drug control, and no drug was used as a blank control, each treatment was repeated 4 times, and cultivated under the same water and fertilizer management conditions. The results were counted after 45 days, and the results were as follows.

[0127] Table 9 Effects of various preparations on root-knot nematode pot experiment

[0128]

[0129]

[0130] From the pot experiment results, it can be seen that the prevention and control effects of lemon grass oil, eugenol, catmint oil, verbena oil, mint oil and benzyl isothiocyanate are 78.32%, 67.56%, 64.56%, 60.33%, 58.09% and 70.11% respectively at the treatment of 200 μl / kg, and all have high prevention and control effects on root-knot nematodes. At the treatment of 200 μl / kg, the prevention and control effects of lemon grass oil: benzyl isothiocyanate (1:1), eugenol: benzyl isothiocyanate (5:1), catmint oil: benzyl isothiocyanate (5:1), verbena oil: benzyl isothiocyanate (3:1) and mint oil: benzyl isothiocyanate (1:5) are 100%, 89.55%, 80.33%, 79.34% and 71.99% respectively, all of which are higher than the prevention and control effect of benzyl isothiocyanate alone (70.11%), which indicates that the plant essential oils and benzyl isothiocyanate have obvious synergistic effect in combination, can reduce the use of single agent and delay resistance.

[0131] Field experiment of five plant essential oils, benzyl isothiocyanate and compound preparation on root-knot nematodes

[0132] The field experiment was carried out in a place where root-knot nematode disease frequently occurs in Dali County, Shaanxi Province (disease index 5 or more). The population of root-knot nematodes in the plot is difficult to control due to long-term planting of single crops. The previous crop planted in the plot was watermelon, and no pesticide was used in the plot one year before the experiment. The plot was planted in single ridge and double rows, and normal ploughing and management were carried out before the experiment without applying any nematicide. Before the experiment, the test pumpkin seedlings were transplanted, and the soil was irrigated with the pesticide diluted with water until the lower 25 cm soil layer was wet. After the treatment, the field ridge was sealed with polyethylene plastic film, and the same method was used to apply water and cover film. After sealing for 7 days, the plot was opened for 4 days, and the pumpkin seedlings were transplanted. The control agent was 20% horseradish water emulsion, and the treatment method was the same. After 60 days of pesticide application, the root-knot index of each treatment was investigated, and the control effect of each pesticide was calculated.

[0133] Table 10 Prevention and control effects of each preparation on root-knot nematodes in field experiment

[0134]

[0135] The field experiment shows that the same 12 g / m 2The field test of the amount of lemon grass oil: benzyl isothiocyanate (1:1) against root-knot nematodes has an efficacy of 80.43%, the field test of the amount of eugenol: benzyl isothiocyanate (5:1) has an efficacy of 75.99%, the field test of the amount of catmint oil: benzyl isothiocyanate (5:1) has an efficacy of 70.82%, the field test of the amount of verbena oil: benzyl isothiocyanate (3:1) has an efficacy of 71.73%, and the field test of the amount of peppermint oil: benzyl isothiocyanate (1:5) has an efficacy of 72.11%, all of which are higher than the use of essential oil and benzyl isothiocyanate alone, and the plant essential oil and benzyl isothiocyanate plant source composition of the application have obvious synergistic effect.

[0136] Example 1: Production of 14% lemon grass oil and 6% benzyl isothiocyanate composition oil agent nemacole A

[0137] Accurately weigh 7 kg of lemon grass oil, and then weigh 3 kg of benzyl isothiocyanate technical material. Mix the two active ingredients in a 2M 3 dispensing tank. Accurately weigh 0.8 kg of ascorbic acid and add it into the dispensing tank (1) and mix again. Then accurately add 25 kg of ethyl formate and 14.2 kg of soybean oil into the dispensing tank, and mix thoroughly. Continue stirring for 1 hour. The prepared 50 kg of lemon grass oil and benzyl isothiocyanate composition oil agent product is obtained. Sampling analysis shows that the content of lemon grass oil is 19.88% and the content of benzyl isothiocyanate is 9.98%. The product is divided into 10 aluminum cans coated with barrier material, each can containing 5 kg. Seal the can and label it as the 29.86% lemon grass oil and benzyl isothiocyanate composition fungicidal and nematocidal oil agent A product.

[0138] Example 2: Production of 30% verbena essential oil and 10% benzyl isothiocyanate composition soluble liquid nemacole B

[0139] Accurately weigh 180 kg of verbena essential oil and 60 kg of benzyl isothiocyanate technical material. Mix the two active ingredients in a 2M 3 dispensing tank. Accurately weigh 0.8 kg of ascorbic acid and add it into the dispensing tank (1) and mix again. Then accurately add 25 kg of ethyl formate and 14.2 kg of soybean oil into the dispensing tank, and mix thoroughly. Continue stirring for 1 hour. The prepared 50 kg of lemon grass oil and benzyl isothiocyanate composition oil agent product is obtained. Sampling analysis shows that the content of lemon grass oil is 19.88% and the content of benzyl isothiocyanate is 9.98%. The product is divided into 10 aluminum cans coated with barrier material, each can containing 5 kg. Seal the can and label it as the 29.86% lemon grass oil and benzyl isothiocyanate composition fungicidal and nematocidal oil agent A product.

[0140] Example 3: Production of 20% eugenol and 8% benzyl isothiocyanate composition emulsifiable concentrate of myclobutanil

[0141] Accurately weigh 64 kg of benzyl isothiocyanate TC, 160 kg of eugenol TC, and 16 kg of ascorbic acid, and mix them well in a preparation tank. Then, add 120 kg of agricultural emulsifier 1601, 250 kg of ethyl formate, and 190 kg of water into the preparation tank while stirring. After all the materials are added, continue stirring for 1 hour. The sample analysis shows that the product contains 7.99% of benzyl isothiocyanate and 19.79% of eugenol, and the total content of effective components is 27.78%. The product is 800 kg, which is sealed in 500 mL glass bottles.

[0142] Example 4: Production of 35% catmint oil and 12% benzyl isothiocyanate composition soluble concentrate of myclobutanil

[0143] Accurately weigh 120 kg of benzyl isothiocyanate TC and 350 kg of catmint oil, and mix them well in a preparation tank (1). Then, accurately weigh 15 kg of ascorbic acid, and add it into the preparation tank (1) to mix well again. In a preparation tank (2), add 60 kg of agricultural emulsifier 500 and 80 kg of emulsifier 1602, mix, then add 375 kg of ethyl formate, and mix well. Move all the 515 kg of materials in the preparation tank (2) into the preparation tank (1) through a pipeline, seal the tank, and stir for 1 hour. The product is 1 ton of catmint oil and benzyl isothiocyanate soluble concentrate. The sample analysis shows that the product contains 12% of benzyl isothiocyanate and 35% of catmint oil, which is 47% of myclobutanil product. The product is divided into double-barrier plastic bottles, each containing 500 mL of the product.

[0144] Example 5: Production of 40% peppermint oil and 15% benzyl isothiocyanate composition microemulsion of myclobutanil

[0145] Accurately weigh 300 kg of benzyl isothiocyanate and 800 kg of peppermint oil. Mix the two active liquids thoroughly in a preparation tank (1). Accurately weigh 40 kg of ascorbic acid and add it to the preparation tank (1) and mix again thoroughly. Add 40 kg of Farm Emulsion 602 and 100 kg of emulsifier OP-10 to a preparation tank (2), mix, then add 720 kg of ethyl formate, and mix thoroughly. Transfer all the contents of the preparation tank (2) to the preparation tank (1) through a pipe, seal the tank, and start the stirrer for 1 hour. Prepare 2000 kg of the peppermint oil-benzyl isothiocyanate soluble liquid product. The content of peppermint oil in the product is 40%, the content of benzyl isothiocyanate is 14.99%, and the product becomes 54.99% of the mycosporine-like amino acid E product. Package the product in double-sealed plastic bottles, with 500 mL of the product in each bottle.

[0146] Example 6: Preparation of 3% lemon grass oil and 3% benzyl isothiocyanate composition granules of mycosporine-like amino acid F

[0147] Accurately weigh 6 kg of benzyl isothiocyanate and 6 kg of lemon grass oil. Mix the two active liquids thoroughly in a preparation tank (1). Accurately weigh 144 kg of starch and add it to the preparation tank (1) and mix again thoroughly. Add 6 kg of carboxymethylcellulose sodium, 19 kg of bentonite, and 19 kg of calcium lignosulfonate to a preparation tank (2), mix thoroughly. Transfer all the contents of the preparation tank (2) to the preparation tank (1) through a pipe, seal the tank, and start the stirrer for 1 hour. Slowly add water several times during this period. When the material can be formed into a ball and crumbled by hand, add all the contents of the preparation tank (1) to an extrusion granulator and granulate. Dry the prepared granules in a drying oven at 60°C for 4 hours to prepare 200 kg of lemon grass oil-benzyl isothiocyanate granules. The content of benzyl isothiocyanate in the granules is 2.98%, the content of lemon grass oil is 2.92%, and the total content of effective components in the product is 5.9%. Package the granules in suitable packaging materials such as aluminum foil bags or plastic bottles.

[0148] Example 7:

[0149] In Shaanxi Weinan vegetable base, pick a piece of cucumber field planted all year round, and long-term serious damage by cucumber root-knot nematode, divided into small area, each area of 30 m2, random arrangement, open the same size hole, each hole using hole irrigation, control agent for 20% horseradish element emulsion, the treatment group agent for 8% benzyl isothiocyanate oil (8% benzyl isothiocyanate + 10% emulsifier 1601 + 82% ethyl acetate), 25% bacteria line kill cabbage element A oil, respectively diluted 2000 times, hole irrigation 250 ml, two months later, the results of statistics, control group, 8% benzyl isothiocyanate oil, 25% bacteria line kill cabbage element A oil, the control effect is 65.87%, 89.45%, 70.11% respectively.

[0150] Example 8:

[0151] In Henan Luoyang vegetable test base, choose a piece of amaranth root-knot nematode infection serious field, divided into small area, random arrangement, each area of 50 m 2 , direct spray, mulching, soil fumigation treatment 7 days, interval 14 days transplant seedlings. Control agent for 20% phosphorothiolate emulsion (jiangsu hui fortune biological agricultural limited company), the treatment group agent for 10% benzyl isothiocyanate oil (10% benzyl isothiocyanate + 8% tween-80 + 5% agricultural emulsion 500 + 50% sec-butyl acetate + 27% ethyl acetate), 40% bacteria line kill cabbage element Y soluble liquid, respectively diluted 1200 times, each square meter of each treatment with drug amount is 15 g, two months later, statistics of each gram of root knot, the control effect is 49.78%, 70.32%, 92.67% respectively.

[0152] Example 9:

[0153] In Foshan Gaoming vegetable base, select a piece of root-knot disease infection serious field, divided into three small area, each area of 100 m 2 . Using ditching way, every 0.6 m open a ditch, set 27.78% emulsion bacteria line kill cabbage element C produced in example 3, 12% benzyl isothiocyanate oil (12% benzyl isothiocyanate + 6% OP-10 + 6% agricultural emulsion 500#+40% ethyl acetate + 42% ethyl formate), water three kinds of treatment, all diluted 1200 times, each ditch dosage is 10 L, cover soil, then put on thick film, airtight seven days, after four days, sow cabbage. After sowing, the management measures of each treatment are the same. To cabbage harvest season, check the root-knot. Water control is 96.89%, 27.78% emulsion bacteria line kill cabbage element C treatment group root-knot infection rate is 6.89%, 12% benzyl isothiocyanate oil treatment root-knot infection rate is 15.32%.

[0154] Example 10:

[0155] The soil with serious watermelon fusarium wilt is put into a bucket, 6 kg of soil is put into each bucket. 60 buckets are prepared, 30 of which are used as blank control and irrigated with 250 mL of clean water. The blank control buckets are wrapped with double nylon film bags. Then 47% soluble liquid Xanthomonas campestris cell line D produced in Example 4 is diluted 1200 times, 250 mL of the diluted solution is irrigated into each bucket, and the soil is covered. Each bucket is wrapped with a nylon bag as soon as possible, and the wrapped buckets are tightly sealed for 7 days. The film is removed, and the buckets are exposed to air for 7 days. Then, watermelon seedlings are transplanted into each bucket, and two seedlings are transplanted into each bucket. Under the same management conditions, the watermelon seedlings are grown for 45 days. 50 of the 60 watermelon seedlings in the blank control die, and 6 of the 60 watermelon seedlings in the soil treated with the agent of the application die, and the control effect is 90%.

[0156] Example 11

[0157] The soil with serious crucifer soft rot infection is mixed uniformly and put into a flowerpot, 1 kg of soil is put into each pot. Then 1.26 g of 7.9% granular Xanthomonas campestris cell line F produced in Example 6 is weighed and uniformly mixed into the soil, and a small amount of clean water is irrigated. The pot and sand treated with the agent are sealed with polyvinyl chloride plastic film for 7 days, and then exposed to air for 4 days. Brassica napus seeds are sown in the flowerpot, and 200 seeds are sown in each of the blank and treatment groups. After half a month, the survival rate is calculated. The survival rate of the control group is almost zero. The mortality rate of the agent treatment group is only 15.78%.

[0158] Although the present application has been described in detail in the foregoing description with general principles and specific embodiments, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application are within the scope of the present application.

Claims

1. A composition comprising a plant essential oil and benzyl isothiocyanate, wherein, The effective components of the composition are lemon grass oil and benzyl isothiocyanate; The weight ratio of the lemon grass oil and benzyl isothiocyanate is 1:20-15:

1.

2. Use of the plant essential oil and benzyl isothiocyanate containing composition of claim 1 for preparing a fumigant for controlling soil-borne pathogens and / or soil nematodes.

3. Use according to claim 2, characterized in that, The soil-borne pathogens include Agrobacterium tumefaciens and / or Fusarium oxysporum f. sp. niveum; The soil nematodes are root knot nematodes.

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