Portable living cell imaging analysis system and method

Abstract

The invention discloses a portable living cell imaging analysis system and method.The system is characterized in that a rectangular cavity is formed in the upper portion of a main body frame, a sample frame support is arranged at the bottom in the rectangular cavity, a sample frame is fixed to the sample frame support, a sample cavity is formed in the sample frame, and a conductive glass plate is arranged in the sample frame; a microscope module is arranged in the main body frame below the through hole, the microscope module is erected in the main body frame through a microscope module support, an LED light source is arranged in the main body frame, and a driving plate is arranged in the main body frame. Through modular and integrated arrangement, the living cell detection device has the capacity of being quickly disassembled and assembled, flexible deployment in different scenes is facilitated, maintenance and upgrading of equipment are simpler and more efficient, and through an integrated temperature control system, an ideal environment needed by cells can be accurately adjusted and maintained. Through combination of a high-precision optical system and an image enhancement technology, high-resolution imaging can be realized.

Description

Technical Field

[0001] The present invention relates to the field of biological detection technology, and in particular to a portable living cell imaging analysis system and method. Background Art

[0002] With the deepening of life science research, live cell detection technology is increasingly playing a key role in the fields of cell biology, disease diagnosis, drug screening, etc. Live cell detection can monitor the dynamic behavior of cells in real time, such as migration, division, apoptosis and other processes, and provide important data on cell physiological changes. However, the current live cell detection system mainly relies on high-resolution equipment such as traditional optical microscopes and fluorescence microscopes. These technologies have irreplaceable advantages in the dynamic observation of cells and the tracking of internal processes, but they are usually bulky, costly, and complex to operate, requiring professionally trained technicians to operate. Therefore, these devices are mostly used in laboratory environments and cannot meet the actual application needs such as rapid on-site detection, primary medical care, and emergency response.

[0003] Although there are some portable microscopes on the market, they are usually only suitable for short-term observation of samples. Especially in live cell detection, existing portable devices often lack sufficient imaging resolution and are difficult to observe and track dynamic cells for a long time. These limitations greatly restrict the application of existing portable microscopes in live cell research. Summary of the invention

[0004] The embodiments of the present invention provide a portable live cell imaging analysis system and method to solve the problems in the prior art that the live cell detection device is large in size, inconvenient to disassemble, difficult to operate and has poor applicability, and cannot perform long-term live cell detection outside the laboratory environment, thereby affecting the experimental efficiency.

[0005] In one aspect, an embodiment of the present invention provides a portable living cell imaging analysis system, comprising:

[0006] The main body frame is a rectangular parallelepiped, a rectangular cavity is arranged on the upper part of the main body frame, a sample holder bracket is arranged at the bottom of the rectangular cavity, a through hole is arranged at the center of the sample holder bracket, telescopic electrodes are arranged on both sides of the through hole, a sample holder is fixed on the sample holder bracket, a fixing groove is arranged on the sample holder, clips are arranged at the four corners of the top of the fixing groove by fixing screws, a sample cavity corresponding to the size of the fixing groove is arranged in the fixing groove, a slot is arranged inside the sample holder, a square through hole is arranged between the slot and the fixing groove, a conductive glass plate is arranged in the slot, and a microscope is arranged inside the main body frame below the through hole. The microscope module is mounted inside the main frame through a microscope module bracket, the microscope module bracket is slidably connected to the main frame through a movable slide, an LED light source is arranged in the main frame above the sample frame, a driving board is arranged in the main frame, a rear cover is arranged behind the main frame, a power interface, a power switch, a temperature control board and a communication interface are arranged on the rear cover, the power interface and the power switch are electrically connected to the driving board, the LED light source, the telescopic electrode and the temperature control board, the temperature probe of the temperature control board is arranged in the fixed groove, and the telescopic electrode is electrically connected to the conductive glass plate.

[0007] In a possible implementation, the main frame and the rear cover are fixedly connected by screws, the top of the rear cover covers the top of the main frame, and the rear cover covers the back opening of the main frame.

[0008] In a possible implementation, the bottom of the sample rack is set to correspond to the size of the top of the sample rack support, and the sample rack is fixedly connected to the main rack support by screws.

[0009] In a possible implementation, the sample through hole, the conductive glass plate, the fixing groove, the square through hole and the LED light source are arranged in the same optical path, the lens of the microscope module is arranged corresponding to the through hole, and the lens of the microscope module is arranged toward the direction of the LED light source.

[0010] In a possible implementation, the microscope module includes a lower base, a data interface, an upper base, an upper adapter, a camera sensor chip, a tube lens, a magnifying glass and a top cover.

[0011] In a possible implementation, the camera sensor chip is arranged between the lower bottom and the upper bottom, the data interface is arranged on the side of the lower bottom, the tube lens is arranged between the upper bottom and the upper adapter, the tube lens is inserted into the upper adapter, the magnifying glass is arranged on the top of the upper adapter, the top cover is covered on the top of the upper adapter, a center through hole is arranged in the center of the top cover, and the size of the center through hole is set corresponding to the size of the magnifying glass.

[0012] In another aspect, an embodiment of the present invention provides a living cell imaging analysis method, comprising:

[0013] Assemble the main frame, sample holder, microscope module and LED light source into a portable live cell imaging analysis system;

[0014] Preheating the sample holder by means of a conductive glass plate;

[0015] Loading a sample through the sample cavity and placing it into the sample rack;

[0016] The photographing of the sample is completed by the autofocus algorithm of the microscope module.

[0017] In a possible implementation, before completing the photographing of the sample by the autofocus algorithm of the microscope module, the method further includes completing enhancement of the sample image by an image enhancement algorithm.

[0018] A portable living cell imaging analysis system and method in the present invention has the following advantages:

[0019] (1) The modular and integrated settings enable the living cell detection device to be quickly disassembled and assembled, facilitating flexible deployment in different scenarios, and making equipment maintenance and upgrades simpler and more efficient.

[0020] (2) Through the integrated temperature control system, the ideal environment required by cells can be accurately adjusted and maintained. This temperature control system can monitor and adjust the temperature inside the device in real time to ensure that the physiological state of cells during the detection process is not affected by external temperature fluctuations, thereby providing cells with a stable and constant culture environment to meet the strict temperature requirements of living cell experiments.

[0021] (3) Through the combination of high-precision optical systems and image enhancement technology, high-resolution imaging can be achieved to clearly display the morphology, position and dynamic behavior of living cells. This enables accurate observation of the basic structure and biological processes of cells, such as cell migration, proliferation and division, and meets the needs of high-precision living cell detection.

[0022] (4) The combination of high-resolution imaging technology and precise temperature control system ensures that the device can still provide high-quality cell detection in various field environments. The device can not only perform dynamic observation for a long time under stable temperature control conditions, but also effectively avoid the interference of external environmental fluctuations on the physiological state of cells, thereby improving the reliability and accuracy of live cell detection. This technology fusion enhances the applicability and convenience of portable devices in field applications, enabling accurate and continuous live cell monitoring in different scenarios. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0024] Figure 1 A schematic diagram of the structure of a portable living cell imaging analysis system provided by an embodiment of the present invention;

[0025] Figure 2 A flow chart of a living cell imaging analysis method provided by an embodiment of the present invention;

[0026] Figure 3 A schematic diagram of the structure of a microscope module of a portable living cell imaging analysis system provided by an embodiment of the present invention;

[0027] Figure 4 An internal schematic diagram of a portable living cell imaging analysis system provided by an embodiment of the present invention;

[0028] Figure 5 A schematic diagram of a sample rack support and a sample rack of a portable living cell imaging analysis system provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] Figure 1 A schematic diagram of the structure of a portable living cell imaging analysis system provided by an embodiment of the present invention; an embodiment of the present invention provides a portable living cell imaging analysis system, comprising:

[0031] The main body frame 1 is a rectangular parallelepiped, a rectangular cavity is arranged on the upper part of the main body frame 1, a sample rack bracket 7 is arranged at the bottom of the rectangular cavity, a through hole is arranged in the center of the sample rack bracket 7, telescopic electrodes 6 are arranged on both sides of the through hole, a sample rack 4 is fixed on the sample rack bracket 7, a fixing groove is arranged on the sample rack 40, clips 9 are arranged at the four corners of the top of the fixing groove by fixing screws 8, a sample cavity 5 corresponding to the size of the fixing groove is arranged in the fixing groove, a slot is arranged inside the sample rack 4, a square through hole is arranged between the slot and the fixing groove, a conductive glass plate 10 is arranged in the slot, a microscope module 13 is arranged inside the main body frame 1 below the through hole, and the microscope module 13 is through A microscope module bracket 11 is erected inside the main frame 1, and the microscope module bracket 11 is slidably connected to the main frame 1 through a movable slide 12. An LED light source 15 is arranged in the main frame 1 above the sample frame 4, and a driving board 4 is arranged in the main frame 1. A rear cover plate 20 is arranged behind the main frame 1, and a power interface 16, a power switch 17, a temperature control board 18 and a communication interface 19 are arranged on the rear cover plate 20. The power interface 16 and the power switch 17 are electrically connected to the driving board 4, the LED light source 15, the telescopic electrode 6, and the temperature control board 18. The temperature probe 28 of the temperature control board 18 is arranged in the fixed groove, and the telescopic electrode 6 is electrically connected to the conductive glass plate 10;

[0032] The main frame 1 is fixedly connected to the rear cover plate 20 by screws, the top of the rear cover plate 20 covers the top of the main frame 1, and the rear cover plate 20 covers the back opening of the main frame 10;

[0033] The bottom of the sample rack 4 is set to correspond to the top of the sample rack support 7, and the sample rack 4 is fixedly connected to the sample rack support 7 by screws;

[0034] The sample through hole, the conductive glass plate 10, the fixing groove, the square through hole and the LED light source 15 are arranged in the same optical path, the lens of the microscope module 13 is arranged corresponding to the through hole, and the lens of the microscope module 13 is arranged toward the direction of the LED light source 15;

[0035] The microscope module 13 includes a lower base 20, a data interface 21, an upper base 22, an upper adapter 23, a camera sensor chip 24, a tube lens 25, a magnifying glass 26 and a top cover 27;

[0036] The camera sensor chip 24 is arranged between the lower bottom 20 and the upper bottom 22, the data interface 21 is arranged on the side of the lower bottom 20, the tube lens 25 is arranged between the upper bottom 22 and the upper adapter 23, the tube lens 25 is inserted into the upper adapter 23, the magnifying glass 26 is arranged on the top of the upper adapter 23, the top cover 27 covers the top of the upper adapter 23, and a central through hole is arranged in the center of the top cover 27, and the size of the central through hole is set corresponding to the size of the magnifying glass 26.

[0037] For example, Figure 3 , 4 5, the temperature probe 28 is arranged in the fixing groove, and is used to sense the temperature of the conductive glass plate 10. The conductive glass plate 10 is an ITO conductive glass. The temperature probe 28 is controlled by the temperature control board 18, and is used to maintain the temperature of the conductive glass plate 10 within the range of living cell survival. The LED light source 15 illuminates the living cell sample in the sample chamber 5 from top to bottom;

[0038] The conductive glass plate 10 is inserted into a slot provided inside the sample rack 4, and the temperature probe 28 is led out from the temperature control board 18 and inserted into a hole reserved in the sample rack, and is placed above the conductive glass plate 10 and below the sample cavity 5. The fixing screw 8 passes through the hole in the clamp 9 and docks with the screw hole on the sample rack 4 to be fixed on the sample rack 4, and is used to fix the sample cavity 5 through the clamp 9. The sample rack 4 has fixed threaded holes on both sides to connect with the fixed threaded holes of the lower sample rack bracket 7;

[0039] The main frame 1 has threaded holes on the top and both sides, the rear cover 20 is fixed on the threaded holes on the top, and the movable slide 12 is fixed on the threaded holes on both sides of the main frame 1; the microscope module bracket 11 is fixed on the threaded holes at both ends of the movable slide 12; the microscope module 13 is fixed on the threaded holes opened below the microscope module bracket 11; the LED light source 15 is fixed in the circular groove at the top of the main frame 1;

[0040] The temperature control board 18 is installed on the hole reserved on the right side of the rear cover 20; the power interface 16 is installed on the circular hole reserved on the left side of the rear cover 20; the power switch 17 is installed on the square hole reserved above the power interface 16; the communication interface 19 has a 5mm circular hole for easy installation of the communication interface line;

[0041] The bottom of the lower base 20 has four threaded holes, which are used to fix the camera sensor chip 24 of the microscope module 13 and connect to the upper base 22. A 15mm round hole is left at the front end for installing the data connection port 21; the upper base 22 has a large threaded hole for screwing the tube lens 25 on it to complete the transfer; the upper transfer 23 is divided into an upper tube and a lower tube, the upper tube is used to install a magnifying glass 26, the magnifying glass 26 is a 30x magnifying glass, and the lower tube is used to connect to the upper base 22; the top cover 27 is used to connect the upper tube of the upper transfer 23 to complete the package;

[0042] A hole is opened below the telescopic electrode 6 for connecting the wire from the lower end of the telescopic electrode 6 to the temperature control board 18; 8 threaded holes are opened on the lower part of the sample rack bracket 7, which are respectively used to connect the sample rack 4 at the upper end and the main frame 1;

[0043] The sample chamber 5 is made of acrylic material, with an overall size of 29mm×26mm×14mm, and has a three-layer structure of upper, middle and lower layers, wherein the upper and lower layers are respectively cylinders with a radius of 8mm and a height of 6mm and a radius of 8mm and a height of 5mm, and the middle layer is connected by a cylinder with a radius of 5mm and a height of 3mm. After affixing a PCR biofilm to the bottom of the sample chamber 5, the sample can be planted into the sample chamber, and then a sealing film is affixed to the upper part of the sample chamber 5 to prevent other microorganisms from entering the sample chamber, so as to achieve long-term cell culture;

[0044] The main frame 1, the rear cover plate 20, the sample rack support 7, the sample rack 4, and the microscope module support 11 are made of photosensitive resin material;

[0045] The temperature probe 28 is an NTC thermistor with a resistance value of 10KΩ, a resistance accuracy of ±0.01, a temperature measurement range of -55°C to 125°C, and a resistance B value of 3950;

[0046] The glass size of the conductive glass plate 10 is smaller than that of a glass slide, and the resistance value is 7-10Ω; it is conductive on one side, and relies on a layer of coating heat to transfer to the sample chamber to provide the temperature required for cell culture, so as to avoid the influence of current on cell activity;

[0047] The movable slide 12 has a lead screw length of 45 mm, a slider length of 34 mm, a lead screw pitch of 0.5 mm (a step of movement of 0.025 mm), a 2-phase 4-wire motor, and a drive voltage of 12 V;

[0048] The power interface 16 is a DC 12V DC power plug; the temperature control board 18 outputs a voltage of 12V, and the temperature accuracy is ±0.1°;

[0049] The microscope module 13 has a length, width and height of 4.2 cm*4.2 mm*7 cm and a volume of <124 cm 3, used for collecting sample data above; the clip 9 is 1.5cm long and 0.5cm wide; the fixing screw 8 is used to pass through the clip 9 and then fixed on the top of the sample rack 4, and then the sample chamber 5 is fixed on the sample rack 4 by screwing, so that the conductive glass plate 10, the temperature probe 28 and the bottom of the sample chamber 5 are fully in contact, so as to measure the real-time temperature in the sample chamber 5 and timely feedback to the temperature control board 18, the temperature control board 18 is connected to the power interface 16, the power switch 17 and the temperature probe 28 through the copper core insulated flame retardant sheathed wire, the required input voltage is 12V, and the output voltage is 12V; the telescopic electrode is used to form a circuit loop on the conductive glass plate 10. The temperature of the temperature control board 18 is set to 37 degrees Celsius. When the temperature probe 28 measures a temperature higher than 37°C, it stops working and the conductive glass plate 10 stops heating. When the temperature is lower than 37°C, it continues to work and the conductive glass plate 10 starts heating.

[0050] In a possible embodiment, the microscope module 13 may also be a microscope with a fixed focal length, and the microscope module 13 with a fixed focal length does not have the movable slide 12 and the driving plate 4 disposed inside the main frame 1 .

[0051] Figure 2 A flow chart of a living cell imaging analysis method provided in an embodiment of the present invention; an embodiment of the present invention provides a living cell imaging analysis method, comprising:

[0052] Assemble the main frame 1, the sample frame 4, the microscope module 13 and the LED light source 15 into a portable living cell imaging analysis system;

[0053] Preheating the sample holder 4 is completed through the conductive glass plate 10;

[0054] Load the sample through the sample chamber 5 and put it into the sample rack 4;

[0055] The photographing of the sample is completed by the autofocus algorithm of the microscope module 13 .

[0056] Exemplarily, first, the device for live cell detection is installed, the power supply is connected, the power switch 17 is turned on, the temperature is set to 37 degrees Celsius for preheating, and then the communication interface 19 and the data interface 21 are connected to the external host computer, that is, the computer control device;

[0057] Secondly, attach the PCR membrane to the lower surface of the sterilized sample cavity 5, plant the cells to be tested into the sample cavity 5, and then seal the upper surface of the sample cavity 5 with a sealing film, and finally place it on the sample rack 4, and then fix it with a clamp 9, and set the temperature to 37°C;

[0058] Again, open the visual data acquisition software, connect the microscope module 13 and the LED light source 15 interface, select the required resolution and white balance state, and use the image enhancement algorithm to process the real-time image in the subsequent imaging stage; connect the Z-axis movement interface, and then the moving slide 12 will automatically focus according to the algorithm, find the best focal plane by judging the clarity value, and stop moving;

[0059] Finally, after connecting all ports, select the number of cycles and interval time according to the actual shooting situation;

[0060] Every time the scheduled shooting time is reached, the LED light source 15 is automatically turned on, the translation stage will move up and down and then find the best focal plane through clarity value evaluation, then data collection is performed, the LED light source 15 is turned off, and finally the collected data is saved in the selected folder.

[0061] In a possible embodiment, before completing the photographing of the sample by the autofocus algorithm of the microscope module 13 , the method further includes completing enhancement of the sample image by an image enhancement algorithm.

[0062] Exemplarily, the image enhancement algorithm includes:

[0063] Step a: Use Gaussian filtering to remove noise, especially high-frequency noise, from the image. The core idea is to perform weighted averaging on the image so that the pixel values ​​of the image are smoothed according to the pixel values ​​of its neighborhood. The Gaussian function formula is as follows:

[0064]

[0065] Among them, σ is the standard deviation of the Gaussian function, which determines the smoothness of the filter. By filtering the image with multiple Gaussian filters with different standard deviations, background information of different scales can be obtained.

[0066] Specific steps:

[0067] First scale filtering: Use a Gaussian filter with a standard deviation of 50 to smooth the grayscale image to obtain the first scale background image. Its purpose is to retain relatively local background information in the image and is suitable for structures with similar cell nuclei. scale1 = imgaussfilt(img_gray,50)

[0068] Second scale filtering: Use a Gaussian filter with a standard deviation of 100 to smooth the grayscale image to obtain a second scale background image. Its purpose is to extract large-scale, low-frequency background information in the image, which is suitable for compensating for uneven global illumination. scale2 = imgaussfilt (img_gray, 100)

[0069] Multi-scale background fusion: By fusing background images of different scales, it is possible to retain local details and smooth the global background to obtain a comprehensive background image. combined_background = (scale1 + scale2) / 2.

[0070] Through the above multi-scale Gaussian filtering, the comprehensive background image is subtracted from the original image, and local features in the image, such as cell nuclei or edges, are extracted, making cell edges and texture features more prominent, providing a clearer input image for subsequent contrast enhancement.

[0071] Step b, perform histogram equalization, by adjusting the grayscale distribution of the image, making the contrast of the image more balanced, thereby enhancing the details. However, traditional histogram equalization will remap the grayscale of the entire image, which may cause excessive contrast enhancement in some local areas and generate noise. To solve this problem, limited contrast adaptive histogram equalization is used. The formula is as follows:

[0072]

[0073] in:

[0074] r k is the grayscale of the input image;

[0075] n i is the number of pixels with gray level i in the image;

[0076] n is the total number of pixels in the image.

[0077] By dividing the image into several small areas (tiles) of fixed size, the histogram of each area is independently equalized to avoid compression of the dark background or saturation of the bright area during global enhancement. In the dark background area, since the original low-contrast grayscale values ​​are concentrated in a lower range, the local grayscale values ​​are redistributed and stretched to a wider range, making weak signals in the dark background (such as cell nuclei or edge structures) more conspicuous and the cell outlines clearly visible. In the highlight area, by limiting the contrast enhancement to prevent oversaturation of bright pixels, and redistributing the grayscale values, the details in the highlight area (such as the texture on the cell membrane) are easier to distinguish, thereby significantly improving the expression of local details of the overall image.

[0078] Step c: enhancing the high-frequency components of the image, especially the edge information in the image, by unsharp masking, so that the edge is more prominent and the image becomes sharper.

[0079] The formula is as follows:

[0080] I sharpened =I+K*(II blur )

[0081] in:

[0082] I sharpened : This is the image after sharpening.

[0083] I is the original image (unprocessed image).

[0084] I blur It is the blurred image, that is, the result of low-pass filtering.

[0085] K is the sharpening coefficient, which controls the strength of the enhancement and is usually a positive value. If k is larger, the sharpening effect is stronger and the edges are more obvious; if k is smaller, the effect is weaker.

[0086] The unsharp masking process can be divided into three steps. First, the low-frequency components of the image are obtained by blurring the original image. The blurred image presents a smooth background and lacks detail information. Next, the blurred image is subtracted from the original image to obtain a difference image, which represents the high-frequency components of the original image, namely the edges and details, and extracts the edge information of the image. Finally, this high-frequency difference image is multiplied by an enhancement coefficient k and added back to the original image, thereby enhancing the edges and details of the image and making the image sharper.

[0087] In summary, multi-scale Gaussian filtering smoothes the global background and highlights local details, making cell edges and texture features clearer; limited contrast adaptive histogram equalization improves the local contrast of dark background and highlight areas, making weak signal areas (such as cell nuclei or edge structures) more conspicuous while avoiding oversaturation of bright areas; unsharp masking further enhances edges and high-frequency details, making cell outlines and internal textures more prominent. The combination of these features provides higher-quality input images for the autofocus algorithm, reduces background interference and noise, and significantly improves the accuracy and efficiency of focusing, especially in low-contrast or complex background scenes.

[0088] The autofocus algorithm includes: the autofocus process starts from the initial position, first controlling the mobile platform to move upward 10 steps, 2μm per step, until it reaches the top. Next, take and save an initial image. Subsequently, the mobile platform moves downward 20 steps, 2μm per step, and gradually takes a series of images. After each image is taken, the Roberts algorithm is applied to evaluate the clarity of the image. Then, the evaluation function is used to comprehensively evaluate the clarity of the 21 images, and the image with the highest clarity is selected, and its focal length is determined as the optimal focus position. Finally, based on the optimal focus position, the distance that needs to be adjusted upward is calculated, and the focal length is accurately adjusted through the mobile platform to ensure that the image reaches the optimal focus state.

[0089] The Roberts algorithm includes: extracting the gradient information of the image by calculating the grayscale difference between each pixel of the image and its lower right neighboring pixel. For a grayscale image I (x, y), the gradients in two directions of the Roberts algorithm are:

[0090] Gradient in x direction:

[0091] G x (x,y)=I(x+1,y+1)-I(x,y)

[0092] Gradient in y direction:

[0093] G y (x,y)=I(x+1,y)-I(x,y+1)

[0094] where Gx and Gy are the gradients in the x and y directions respectively.

[0095] The final edge gradient is the sum of the squares of the gradients in these two directions:

[0096]

[0097] The image clarity evaluation process includes three steps: first, capture the image and convert it into a grayscale image to remove color information to simplify calculations; second, apply the Roberts algorithm to calculate the gradient strength of each pixel in the image to extract the edge information of the image; finally, evaluate the clarity by calculating the sum of the gradient strengths of all pixels in the image, using the formula:

[0098]

[0099] Among them, C is the clarity value of the image, and G(x,y) is the gradient strength of each pixel. The image clarity score helps to determine whether the image is in focus, so as to complete the focus.

[0100] Although the preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0101] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A portable living cell imaging analysis system, characterized in that: include: A main frame (1), wherein the main frame (1) is a rectangular parallelepiped, a rectangular cavity is arranged on the upper part of the main frame (1), a sample frame bracket (7) is arranged at the bottom of the rectangular cavity, a through hole is arranged at the center of the sample frame bracket (7), telescopic electrodes (6) are arranged on both sides of the through hole, a sample frame (4) is fixed on the sample frame bracket (7), a fixing groove is arranged on the sample frame (40), clips (9) are arranged at the four corners of the top of the fixing groove through fixing screws (8), a sample cavity (5) corresponding to the size of the fixing groove is arranged in the fixing groove, a slot is arranged inside the sample frame (4), a square through hole is arranged between the slot and the fixing groove, a conductive glass plate (10) is arranged in the slot, a microscope module (13) is arranged inside the main frame (1) below the through hole, and the microscope module (13) is connected to the microscope module bracket (1 1) is mounted inside the main frame (1), the micromirror module bracket (11) is slidably connected to the main frame (1) via a movable slide (12), an LED light source (15) is arranged in the main frame (1) above the sample frame (4), a driving board (4) is arranged in the main frame (1), a rear cover plate (20) is arranged behind the main frame (1), a power interface (16), a power switch (17), a temperature control board (18) and a communication interface (19) are arranged on the rear cover plate (20), the power interface (16) and the power switch (17) are electrically connected to the driving board (4), the LED light source (15), the telescopic electrode (6) and the temperature control board (18), a temperature probe (28) of the temperature control board (18) is arranged in the fixing groove, and the telescopic electrode (6) is electrically connected to the conductive glass plate (10).

2. A portable living cell imaging analysis system according to claim 1, characterized in that: The main frame (1) and the rear cover plate (20) are fixedly connected by screws; the top of the rear cover plate (20) covers the top of the main frame (1); and the rear cover plate (20) covers the back opening of the main frame (10).

3. A portable living cell imaging analysis system according to claim 1, characterized in that: The bottom of the sample rack (4) is set to correspond to the size of the top of the sample rack support (7), and the sample rack (4) and the rack support (7) are fixedly connected by screws.

4. The portable living cell imaging analysis system according to claim 1, characterized in that: The sample through hole, the conductive glass plate (10), the fixing groove, the square through hole and the LED light source (15) are arranged in the same optical path, the lens of the microscope module (13) is arranged corresponding to the through hole, and the lens of the microscope module (13) is arranged in the direction of the LED light source (15).

5. A portable living cell imaging analysis system according to claim 4, characterized in that: The microscope module (13) comprises a lower base (20), a data interface (21), an upper base (22), an upper adapter (23), a camera sensor chip (24), a tube lens (25), a magnifying glass (26) and a top cover (27).

6. A portable living cell imaging analysis system according to claim 5, characterized in that: The camera sensor chip (24) is arranged between the lower bottom (20) and the upper bottom (22), the data interface (21) is arranged on the side of the lower bottom (20), the tube lens (25) is arranged between the upper bottom (22) and the upper adapter (23), the tube lens (25) is inserted into the upper adapter (23), the magnifying glass (26) is arranged on the top of the upper adapter (23), the top cover (27) covers the top of the upper adapter (23), and a central through hole is arranged at the center of the top cover (27), and the size of the central through hole is set corresponding to the size of the magnifying glass (26).

7. A method for live cell imaging analysis, characterized in that: include: Assembling a main frame (1), a sample frame (4), a microscope module (13) and an LED light source (15) into a portable living cell imaging analysis system; Preheating the sample holder (4) is completed through a conductive glass plate (10); Loading a sample through the sample chamber (5) and placing it into the sample rack (4); The photographing of the sample is completed by the automatic focusing algorithm of the microscope module (13).

8. A living cell imaging analysis method according to claim 7, characterized in that: Before completing the photographing of the sample by the autofocus algorithm of the microscope module (13), the method also includes completing the enhancement of the sample image by an image enhancement algorithm.

Images