Automatic gel cutting and recycling device for nucleic acid electrophoresis gel

Abstract

The invention discloses an automatic gel cutting and recycling device for nucleic acid electrophoresis gel and relates to the field of biological experiment instruments. The invention aims to solve the problems that the precision is inconsistent and operators are easy to hurt when nucleic acid electrophoresis gel is recovered manually in the prior art. According to the automatic gel cutting and recycling device for the nucleic acid electrophoresis gel, the gel cutting platform is used for bearing the nucleic acid electrophoresis gel, the gel cutting platform is located below the driving assembly, and the gel cutting assembly is located on the driving assembly; the driving assembly can drive the rubber cutting assembly to move in three three-dimensional directions, wherein every two of the three-dimensional directions are perpendicular to each other, so that the rubber cutting action is achieved. According to the invention, the machine vision is used for identifying the nucleic acid target strip, and the gel cutting recovery is automatically carried out, so that the inconsistency of manual operation can be avoided, and the injury to personnel can also be avoided.

Description

Technical Field

[0001] The invention belongs to the field of biological experimental instruments, and in particular relates to a nucleic acid electrophoresis gel automatic gel cutting and recovery device. Background Art

[0002] In biological experiments, the size sorting of nucleic acid fragments is usually carried out by vertical electrophoresis and horizontal electrophoresis. The target bands are obtained by cutting and recovering the polyacrylamide gel and agarose gel after electrophoresis. Currently, most experiments place the nucleic acid gel on a transparent UV gel-cutting platform, and the experimenter reaches in from the side through the protective plate to cut the gel manually. The experimenter carefully identifies and cuts the target bands under ultraviolet light, which is not only time-consuming and relatively cumbersome, but also prone to nucleic acid contamination and enzyme degradation. Subjective and technical deviations between different experimenters lead to different sizes of target bands, affecting the accuracy of the experiment. Prolonged exposure of experimenters to ultraviolet rays can cause damage to the skin and eyes, and sharp instruments such as scalpels used in the gel cutting process also pose risks of accidental injuries. Summary of the invention

[0003] The invention aims to solve the problem that the existing manual recovery of nucleic acid electrophoresis gel has inconsistent accuracy and is easy to cause harm to operators. A nucleic acid electrophoresis gel automatic gel cutting and recovery device is provided.

[0004] A nucleic acid electrophoresis gel automatic cutting and recycling device, comprising: a driving component 2, a gel cutting platform 4 and a gel cutting component 6;

[0005] The gel cutting platform 4 is used to carry the nucleic acid electrophoresis gel 43. The gel cutting platform 4 is located below the driving component 2. The gel cutting component 6 is located on the driving component 2. The driving component 2 can drive the gel cutting component 6 to move in three three-dimensional directions that are perpendicular to each other to achieve gel cutting.

[0006] Furthermore, the driving assembly 2 includes: a Y-axis driving module 23, an X-axis driving module 24 and a Z-axis driving module 25;

[0007] The Y-axis driving module 23 includes two slideways arranged parallel to each other, the X-axis driving module 24 and the Z-axis driving module 25 each include a slideway, the two ends of the slideway of the X-axis driving module 24 are respectively connected to the sliders of the two slideways of the Y-axis driving module 23, and the slideway of the X-axis driving module 24 is perpendicular to the two slideways of the Y-axis driving module 23, the slideway of the Z-axis driving module 25 is connected to the slider of the slideway of the X-axis driving module 24, and the slideway of the Z-axis driving module 25 is perpendicular to the plane formed by the slideways of the Y-axis driving module 23 and the X-axis driving module 24, and the rubber cutting component 6 is located on the slider of the slideway of the Z-axis driving module 25.

[0008] Furthermore, the above-mentioned driving component 2 also includes: a workbench 21 and a driving component bracket 22, the Y-axis driving module 23 is fixed above the workbench 21 through the driving component bracket 22, the plane formed by the slide of the Y-axis driving module 23 and the slide of the X-axis driving module 24 is parallel to the plane of the workbench 21, and the glue cutting platform 4 is located on the workbench 21.

[0009] Furthermore, the rubber cutting assembly 6 comprises: a rubber cutting fixing bracket 61, a buffer spring 62, a fixing rod 63, a pneumatic joint 64 and a rubber fixing ring 65;

[0010] The pneumatic joint 64 is fixed to the Z-axis drive module 25 through the rubber cutting fixing bracket 61. The pneumatic joint 64 is connected to one end of the fixing rod 63 through the buffer spring 62. An air passage is provided inside the fixing rod 63 and one end of the air passage opens at the other end of the fixing rod 63. The other end of the fixing rod 63 is conical and is used to be connected to the rubber cutting head 32. The air port of the pneumatic joint 64 is connected to the air passage of the fixing rod 63. The rubber fixing ring 65 is sleeved on the other end of the fixing rod 63.

[0011] Furthermore, the rubber cutting head 32 comprises: a rubber cutting head body 321 and a rubber cutting head piston 322;

[0012] The rubber cutting head body 321 is a tubular structure, and the rubber cutting head piston 322 is located inside the rubber cutting head body 321 and can move along the axial direction of the rubber cutting head body 321, so that the internal space of the rubber cutting head body 321 is divided into two parts with variable space volumes.

[0013] Furthermore, the above-mentioned rubber cutting platform 4 includes: a rotation driving module 41 and a blue light operation platform 42;

[0014] The blue light operation platform 42 is fixed to the upper surface of the workbench 21 through the rotation driving module 41 , so that the blue light operation platform 42 can rotate on the upper surface of the workbench 21 .

[0015] Furthermore, the above-mentioned nucleic acid electrophoresis gel automatic cutting and recovery device also includes a visual detection component 5 , which is located on the Z-axis driving module 25 and is used to collect the image of the nucleic acid electrophoresis gel 43 .

[0016] Furthermore, the visual inspection component 5 includes: a camera mounting bracket 51 and an industrial area array camera 52;

[0017] The industrial area array camera 52 is fixed on the Z-axis driving module 25 via the camera mounting bracket 51 , and the lens 53 of the industrial area array camera 52 faces the rubber cutting platform 4 .

[0018] Furthermore, the above-mentioned nucleic acid electrophoresis gel automatic cutting and recycling device also includes a consumable component 3 located on the upper surface of the workbench 21, and the consumable component 3 includes: a cutting head rack 31, a cutting head recycling box 33 and a gel block recycling tube rack 34;

[0019] The rubber cutting head rack 31 is used to place unused rubber cutting heads 32 , the rubber cutting head recovery box 33 is used to place used rubber cutting heads 32 , and the rubber block recovery tube rack 34 is used to place rubber block recovery tubes 35 .

[0020] Furthermore, the above-mentioned nucleic acid electrophoresis gel automatic cutting and recovery device also includes a box 1;

[0021] The driving assembly 2 , the rubber cutting platform 4 and the rubber cutting assembly 6 are all located inside the box 1 , and the box 1 is provided with a cabinet door 11 .

[0022] The beneficial effects of the nucleic acid electrophoresis gel automatic cutting and recovery device described in the present invention are:

[0023] 1. Use machine vision to identify nucleic acid target bands and automatically perform gel cutting and recovery to avoid nucleic acid contamination and enzyme degradation during the operation and ensure the purity of the sample.

[0024] 2. It can accurately cut nucleic acid bands to ensure the consistency of the size of nucleic acid target bands, improve the accuracy of the experiment, ensure the consistency and repeatability of the experimental results, and reduce the error of the experimental system.

[0025] 3. Automated cutting is easy to operate and reduces the skill requirements for operators.

[0026] 4. It can process multiple samples at the same time, speed up the gel cutting speed, improve the experimental efficiency, and adapt to the requirements of large-scale experiments. At the same time, it can automatically record the cutting position for subsequent analysis.

[0027] 5. Compared with ultraviolet light, blue light is less harmful to the human body and protects the health and safety of experimenters. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is the overall structure diagram of the automatic rubber cutting and recycling device;

[0029] Figure 2 It is an axial side view of the automatic rubber cutting and recycling device;

[0030] Figure 3 It is a front view of the automatic rubber cutting and recycling device;

[0031] Figure 4 is an axial view of the drive assembly;

[0032] Figure 5 is an axonometric view of a consumable assembly;

[0033] Figure 6 It is a front view of the rubber cutting platform;

[0034] Figure 7 It is a top view of the rubber cutting platform;

[0035] Figure 8 This is the right view of the third-direction Z drive module;

[0036] Fig. 9 It is a structural diagram of the visual detection component;

[0037] Fig.10 It is a structural diagram of the cutting rubber component;

[0038] Fig.11 It is the axial side view of the rubber cutting head;

[0039] Among them: 1. Box body; 2. Drive assembly; 3. Consumables assembly; 4. Glue cutting platform; 5. Visual inspection assembly; 6. Glue cutting assembly; 11. Cabinet door; 21. Workbench; 22. Drive assembly bracket; 23. Y-axis drive module; 24. X-axis drive module; 25. Z-axis drive module; 31. Glue cutting head rack; 32. Glue cutting head; 33. Glue cutting head recovery box; 34. Glue block recovery tube rack; 35. Glue block recovery tube; 41. Rotary drive module; 42. Blue light operation platform; 43. Nucleic acid electrophoresis gel; 51. Camera mounting bracket; 52. Industrial array camera; 53. Lens; 61. Glue cutting fixing bracket; 62. Buffer spring; 63. Fixed rod; 64. Pneumatic joint; 65. Rubber fixing ring; 321. Glue cutting head body; 322. Glue cutting head piston. DETAILED DESCRIPTION

[0040] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention. It should be noted that the embodiments of the present invention and the features in the embodiments can be combined with each other without conflict.

[0041] Reference Figures 1 to 11 Specifically describing this embodiment, a nucleic acid electrophoresis gel automatic cutting and recovery device described in this embodiment includes: a box body 1, a driving component 2, a consumable component 3, a gel cutting platform 4, a visual detection component 5 and a gel cutting component 6.

[0042] like Figure 2 and Figure 3As shown, the gel cutting platform 4 is used to carry the nucleic acid electrophoresis gel 43, and the gel cutting platform 4 is located below the driving component 2. The visual detection component 5 and the gel cutting component 6 are both located on the driving component 2, and the visual detection component 5 is used to collect the image of the nucleic acid electrophoresis gel 43. The driving component 2 can drive the gel cutting component 6 to move in three mutually perpendicular three-dimensional directions to achieve gel cutting. The consumable component 3 is placed side by side with the gel cutting platform 4.

[0043] like Figure 1 As shown, the driving component 2, consumable component 3, glue cutting platform 4, visual detection component 5 and glue cutting component 6 are all located inside the box 1. The box 1 adopts a sheet metal structure, which plays a role in equipment support and safety protection. The lower part is the equipment power supply, air source and controller part, which adopts a closed form to protect the internal electrical components from interference and damage from the external environment, and at the same time prevent personnel from operating safely. The upper part is the visual recognition automatic glue cutting and recovery part, which adopts a semi-closed form, with yellow transparent cabinet doors 11 installed on the front and sides, and a blue light filter film is affixed to the surface of the cabinet door 11, which can not only facilitate the observation and operation of the experimenter, but also prevent blue light from damaging the eyes and skin of the experimenter.

[0044] like Figure 4As shown, the driving assembly 2 includes: a workbench 21, a driving assembly bracket 22, a Y-axis driving module 23, an X-axis driving module 24 and a Z-axis driving module 25. The Y-axis driving module 23 includes two slideways arranged in parallel with each other, the X-axis driving module 24 and the Z-axis driving module 25 each include a slideway, the two ends of the slideway of the X-axis driving module 24 are respectively connected to the sliders of the two slideways of the Y-axis driving module 23, and the slideway of the X-axis driving module 24 is perpendicular to the two slideways of the Y-axis driving module 23, the slideway of the Z-axis driving module 25 is connected to the slider of the slideway of the X-axis driving module 24, and the slideway of the Z-axis driving module 25 is perpendicular to the plane formed by the slideways of the Y-axis driving module 23 and the slideways of the X-axis driving module 24, and the rubber cutting assembly 6 is located on the slider of the slideway of the Z-axis driving module 25. The Y-axis driving module 23 is fixed above the workbench 21 through the driving component bracket 22. The plane formed by the slideway of the Y-axis driving module 23 and the slideway of the X-axis driving module 24 is parallel to the plane of the workbench 21, and the glue cutting platform 4 is located on the workbench 21. The workbench 21 is located in the middle of the equipment box and plays a bearing role. The driving component bracket 22 is fixed on the workbench with one group on the left and one group on the right to play the role of bearing the driving module. The Y-axis driving module 23 is arranged on the driving component bracket 22, and the fixed part is detachably connected to the driving component bracket, with one group on the left and one group on the left to play the role of horizontal movement in the Y-axis direction. The fixed part of the X-axis driving module 24 is fixedly connected to the sliding part of the Y-axis driving module, which plays the role of horizontal movement in the X-axis direction. The fixed part of the Z-axis driving module 25 is fixedly connected to the sliding part of the X-axis driving module 24, which plays the role of vertical movement in the Z-axis direction.

[0045] like Figure 5 As shown, the consumable component 3 includes: a rubber cutting head rack 31, a rubber cutting head recovery box 33 and a rubber block recovery tube rack 34. The rubber cutting head rack 31 is used to place unused rubber cutting heads 32, the rubber cutting head recovery box 33 is used to place used rubber cutting heads 32, and the rubber block recovery tube rack 34 is used to place rubber block recovery tubes 35. The rubber cutting head rack 31 is arranged on a workbench, connected by a card slot, and plays a role in fixing the rubber cutting head. The rubber cutting head 32 is arranged on the rubber cutting head rack, and is fixed in the corresponding hole through a jack connection, and is used to extract the target nucleic acid band in the nucleic acid electrophoresis gel. The rubber cutting head recovery box 33 is arranged on the workbench, and is connected by a card slot to recycle the rubber cutting head after use. The rubber block recovery tube rack 34 is arranged on the workbench, and is connected by a card slot to fix the rubber block recovery tube 35. The rubber block recovery tube 35 is arranged on the rubber block recovery tube rack 34, and is fixed in the corresponding hole through a jack connection, and is used to store the recovered rubber blocks.

[0046] like Fig.11As shown, the rubber cutting head 32 includes: a rubber cutting head body 321 and a rubber cutting head piston 322. The rubber cutting head body 321 is a tubular structure, and the rubber cutting head piston 322 is located inside the rubber cutting head body 321 and can move along the axial direction of the rubber cutting head body 321, so that the internal space of the rubber cutting head body 321 is divided into two parts with variable space volumes. The rubber cutting head 32 includes a rubber cutting head body 321 and a rubber cutting head piston 322; the rubber cutting head body 321 plays a role in extracting nucleic acid electrophoresis gel; the rubber cutting head piston 322 is arranged inside the rubber cutting head body, and acts as a negative pressure for the inner cavity of the rubber cutting head body.

[0047] like Figure 6 and Figure 7 As shown, the gel cutting platform 4 includes: a rotation drive module 41 and a blue light operation platform 42. The blue light operation platform 42 is fixed to the upper surface of the workbench 21 through the rotation drive module 41, so that the blue light operation platform 42 can rotate on the upper surface of the workbench 21. The rotation drive module 41 is set on the workbench, and the fixed part is detachably connected to the workbench to play the role of rotating the blue light operation platform 42. The blue light operation platform 42 is set on the rotating part of the rotation drive module 41, with a blue light source at the bottom and a transparent operation surface at the top for carrying the nucleic acid electrophoresis gel 43.

[0048] As shown Figure 8 and 9 As shown, the visual inspection component 5 includes: a camera mounting bracket 51 and an industrial array camera 52. The industrial array camera 52 is fixed to the Z-axis drive module 25 through the camera mounting bracket 51, and the lens 53 of the industrial array camera 52 faces the glue cutting platform 4. The camera mounting bracket 51 is arranged on the side of the fixed part of the Z-axis drive module 25, and is used to fix the industrial array camera 52. The industrial array camera 52 is arranged on the camera mounting bracket 51, and is detachably connected to the camera mounting bracket 51. The shooting end of the industrial array camera 52 is installed downward to collect images. The lens 53 is arranged on the shooting end of the industrial array camera 52, and the axis of the lens 53 is arranged colinearly with the axis of the shooting end of the industrial camera, and cooperates with the industrial array camera 52 to collect images.

[0049] like Figure 8 and 10As shown, the rubber cutting assembly 6 includes: a rubber cutting fixed bracket 61, a buffer spring 62, a fixed rod 63, a pneumatic joint 64 and a rubber fixed ring 65. The pneumatic joint 64 is fixed to the Z-axis driving module 25 through the rubber cutting fixed bracket 61, and the pneumatic joint 64 is connected to one end of the fixed rod 63 through the buffer spring 62. An airway is provided inside the fixed rod 63, and one end of the airway is opened at the other end of the fixed rod 63. The other end of the fixed rod 63 is conical and is used to be connected to the rubber cutting head 32. The air port of the pneumatic joint 64 is connected to the airway of the fixed rod 63, and the rubber fixed ring 65 is sleeved on the other end of the fixed rod 63. The rubber cutting fixed bracket 61 is arranged on the sliding part of the Z-axis driving module 25, and is used to fix the fixed rod 63. The fixed rod 63 is arranged at the lower end of the rubber cutting fixed bracket 61, and is used to adsorb and fix the rubber cutting head 32. The buffer spring 62 is arranged between the fixed part and the sliding part of the fixed rod 63, and plays a role in rubber cutting buffering. The pneumatic joint 64 is arranged at the top of the rubber cutting rod 63 to provide air pressure. The rubber fixing ring 65 is arranged at the conical position at the bottom of the fixing rod 63 to seal the rubber cutting head 32.

[0050] The automatic gel cutting and recovery device for nucleic acid electrophoresis gel described in this embodiment has the following working process:

[0051] The nucleic acid electrophoresis gel is placed on the blue light operation platform 42, and the yellow transparent cabinet door 11 is closed. The blue light of the blue light operation platform 42 is turned on, and the blue light excites the fluorescent dye, so that the nucleic acid bands can be clearly observed.

[0052] The initial position of the visual detection component 5 is above the blue light operation platform 42, the focus plane of the lens 53 is focused on the nucleic acid electrophoresis gel, and the upper surface of the consumable component 3 is on the same plane as the electrophoresis gel, ensuring that they are all within the depth of field of the lens 53, so that the collected pictures can be clear enough. The visual detection component 5 is fixed to the fixed part of the Z-axis drive module 25, and the focus distance will not change with the movement of the XYZ three-axis direction.

[0053] The visual detection component 5 takes pictures of the nucleic acid electrophoresis gel, calculates the rotation angle of the gel cutting platform 4 by recognizing the edge of the gel, and the rotation drive module 41 rotates accordingly according to the deviation angle of the nucleic acid electrophoresis gel. The direction of the nucleic acid electrophoresis gel is the same as the gel cutting direction of the gel cutting head 32.

[0054] The visual detection component 5 identifies the target nucleic acid stripe to confirm the position of the target nucleic acid stripe, and moves the gel cutting component 6 to the top of the target nucleic acid stripe through the movement of the X-axis driving module 24 and the Y-axis driving module 23.

[0055] The Z-axis drive module 25 drives the rubber cutting assembly 6 to move downward, and the rubber cutting head 32 presses the target nucleic acid strip into the front of the rubber cutting head 32. The rubber fixing ring 65, the rubber cutting head body 321, and the rubber cutting head piston 322 form a first closed cavity, and the target nucleic acid strip, the rubber cutting head body 321, and the rubber cutting head piston 322 form a second closed cavity. The pneumatic joint 64 performs negative pressure adsorption, and the air pressure in the first cavity is reduced, so that the rubber cutting head piston 322 moves upward. The upward movement of the rubber cutting head piston 322 forms a negative pressure in the second cavity, so that the target nucleic acid strip rises to prevent the target nucleic acid strip from falling.

[0056] The Z-axis driving module 25 moves upward, and the X-axis driving module 24 and the Y-axis driving module 23 move the rubber cutting component 6 to above the rubber block recovery tube rack 34 , and through positioning and identification by the visual detection component 5 , the rubber cutting head 32 is moved to above the first rubber block recovery tube 35 .

[0057] The Z-axis drive module 25 moves downward, the pneumatic connector 64 increases the air pressure, and the air pressure in the first cavity increases, causing the rubber cutting head piston 322 to move downward. The rubber cutting head piston 322 moves downward, causing the air pressure in the second cavity to increase, causing the target nucleic acid band to be pressed out of the rubber cutting head body 321 and enter the rubber block recovery tube 35 for storage.

[0058] The X-axis driving module 24 and the Y-axis driving module 23 move the rubber cutting assembly 6 to the top of the rubber cutting head 32 recovery box, the pneumatic joint 64 increases the air pressure, and the rubber cutting head piston 322 continues to move downward to the bottom of the rubber cutting head body 321. The first cavity continues to increase the air pressure, and the rubber cutting head 32 and the rubber fixing ring 65 fall off and fall into the rubber head recovery box 33.

[0059] The X-axis driving module 24 and the Y-axis driving module 23 move the rubber cutting assembly 6 to the top of the rubber cutting head 32 rack, and through the positioning and recognition of the visual detection component 5, the rubber cutting assembly 6 is moved to the top of the first rubber cutting head 32. The Z-axis driving module 25 moves downward to press the rubber fixing ring 65 into the rubber cutting head 32, and the Z-axis driving module 25 moves upward to take the rubber cutting head 32 out of the rubber cutting head 32 rack.

[0060] The X-axis driving module 24 and the Y-axis driving module 23 move the gel cutting assembly 6 to above the blue light operation platform 42 and repeat the previous actions until all target nucleic acid bands on the nucleic acid electrophoresis gel are completely recovered.

[0061] Although the present invention is described herein with reference to specific embodiments, it should be understood that these embodiments are merely examples of the principles and applications of the present invention. It should therefore be understood that many modifications may be made to the exemplary embodiments and that other arrangements may be devised without departing from the spirit and scope of the present invention as defined by the appended claims. It should be understood that the various dependent claims and features described herein may be combined in a manner different from that described in the original claims. It should also be understood that the features described in conjunction with a single embodiment may be used in other described embodiments.

Claims

1. A nucleic acid electrophoresis gel automatic cutting and recovery device, characterized in that: include: A driving assembly (2), a rubber cutting platform (4) and a rubber cutting assembly (6); The gel cutting platform (4) is used to carry the nucleic acid electrophoresis gel (43); the gel cutting platform (4) is located below the driving component (2); the gel cutting component (6) is located on the driving component (2); the driving component (2) can drive the gel cutting component (6) to move in three mutually perpendicular three-dimensional directions to achieve gel cutting.

2. The nucleic acid electrophoresis gel automatic cutting and recovery device according to claim 1, characterized in that: The driving assembly (2) comprises: a Y-axis driving module (23), an X-axis driving module (24) and a Z-axis driving module (25); The Y-axis driving module (23) comprises two slideways arranged in parallel with each other, the X-axis driving module (24) and the Z-axis driving module (25) each comprise a slideway, the two ends of the slideway of the X-axis driving module (24) are respectively connected to the sliders of the two slideways of the Y-axis driving module (23), and the slideway of the X-axis driving module (24) is perpendicular to the two slideways of the Y-axis driving module (23), the slideway of the Z-axis driving module (25) is connected to the slider of the slideway of the X-axis driving module (24), and the slideway of the Z-axis driving module (25) is perpendicular to the plane formed by the slideway of the Y-axis driving module (23) and the slideway of the X-axis driving module (24), and the rubber cutting assembly (6) is located on the slider of the slideway of the Z-axis driving module (25).

3. The nucleic acid electrophoresis gel automatic cutting and recovery device according to claim 2, characterized in that: The driving component (2) further includes: a workbench (21) and a driving component bracket (22); the Y-axis driving module (23) is fixed above the workbench (21) through the driving component bracket (22); the plane formed by the slideway of the Y-axis driving module (23) and the slideway of the X-axis driving module (24) is parallel to the plane of the workbench (21); and the glue cutting platform (4) is located on the workbench (21).

4. A nucleic acid electrophoresis gel automatic cutting and recovery device according to claim 2 or 3, characterized in that: The rubber cutting assembly (6) comprises: a rubber cutting fixing bracket (61), a buffer spring (62), a fixing rod (63), a pneumatic joint (64) and a rubber fixing ring (65); The pneumatic joint (64) is fixed to the Z-axis driving module (25) through the rubber cutting fixing bracket (61); the pneumatic joint (64) is connected to one end of the fixing rod (63) through the buffer spring (62); an air passage is provided inside the fixing rod (63) and one end of the air passage opens at the other end of the fixing rod (63); the other end of the fixing rod (63) is conical and is used to be connected to the rubber cutting head (32); the air port of the pneumatic joint (64) is connected to the air passage of the fixing rod (63); and the rubber fixing ring (65) is sleeved on the other end of the fixing rod (63).

5. The nucleic acid electrophoresis gel automatic cutting and recovery device according to claim 4, characterized in that: The rubber cutting head (32) comprises: a rubber cutting head body (321) and a rubber cutting head piston (322); The rubber cutting head body (321) is a tubular structure, and the rubber cutting head piston (322) is located inside the rubber cutting head body (321) and can move along the axial direction of the rubber cutting head body (321), so that the internal space of the rubber cutting head body (321) is divided into two parts with variable spatial volumes.

6. The nucleic acid electrophoresis gel automatic cutting and recovery device according to claim 3, characterized in that: The rubber cutting platform (4) comprises: a rotation driving module (41) and a blue light operation platform (42); The blue light operating platform (42) is fixed to the upper surface of the workbench (21) via the rotation drive module (41), so that the blue light operating platform (42) can rotate on the upper surface of the workbench (21).

7. The nucleic acid electrophoresis gel automatic cutting and recovery device according to claim 2, characterized in that: It also includes a visual detection component (5), which is located on the Z-axis driving module (25) and is used to collect an image of the nucleic acid electrophoresis gel (43).

8. The nucleic acid electrophoresis gel automatic cutting and recovery device according to claim 7, characterized in that: The visual inspection component (5) comprises: a camera mounting bracket (51) and an industrial array camera (52); The industrial area array camera (52) is fixed on the Z-axis driving module (25) via the camera mounting bracket (51), and the lens (53) of the industrial area array camera (52) faces the rubber cutting platform (4).

9. The nucleic acid electrophoresis gel automatic cutting and recovery device according to claim 3, characterized in that: It also includes a consumables assembly (3) located on the upper surface of the workbench (21), and the consumables assembly (3) includes: a rubber cutting head rack (31), a rubber cutting head recovery box (33) and a rubber block recovery tube rack (34); The rubber cutting head rack (31) is used for placing unused rubber cutting heads (32), the rubber cutting head recovery box (33) is used for placing used rubber cutting heads (32), and the rubber block recovery tube rack (34) is used for placing rubber block recovery tubes (35).

10. The nucleic acid electrophoresis gel automatic cutting and recovery device according to claim 1, 2, 3, 5, 6, 7, 8 or 9, characterized in that: It also includes a box (1); The driving component (2), the rubber cutting platform (4) and the rubber cutting component (6) are all located inside the box (1), and the box (1) is provided with a cabinet door (11).

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