Feed additive for improving immunity of dairy cows in perinatal period

Abstract

The invention discloses a feed additive for improving the immunity of dairy cows in the perinatal period, and belongs to the technical field of nutritional feeds, the feed additive comprises the following components: nutritional additives: 500-1000 IU / kg of vitamin E, 0.1-0.3 mg / kg of selenium, 30-50 mg / kg of zinc, 10-20 mg / kg of copper, 20-40 mg / kg of manganese, 0.95-1.1 mmol / L of calcium and 10-20 mL / kg of linseed oil; and the non-nutritional additive comprises the following components: 1 to 5 * 10 < 9 > CFU / kg of lactic acid bacteria, 5 to 10 g / kg of prebiotics, 50 to 100 mg / kg of rosemary extract, 50 to 100 mg / kg of green tea extract and 50 to 100 mg / kg of yeast extract. Vitamin E, selenium, zinc, copper, manganese and other nutrients in the nutritional additive can enhance the oxidation resistance and immune function of dairy cows, so that various diseases including oxidative stress related diseases, infectious diseases, reproductive disorders, skeleton diseases and the like can be prevented.

Description

Technical Field

[0001] The present invention relates to the technical field of nutritional feed, and specifically to a feed additive for improving the immunity of dairy cows during the peripartum period. Background Art

[0002] The peripartum period of dairy cows covers 21 days before calving to 21 days after calving. This period is crucial for the feeding and management of dairy cows as it marks the transition of dairy cows from the dry period to the early lactation period. During this period, the immune system of dairy cows may experience a certain degree of decline, especially at two specific times: one is just after the dairy cow gives birth to a calf, and the other is just after the dairy cow ends the dry period.

[0003] This reduction in immunity may be affected by a variety of internal and external factors. These include the energy balance state, the levels of ketone bodies and free fatty acids in the blood, the calcium metabolism status, the level of glucocorticoids, and the degree of stress borne by the dairy cows. In addition, nutritional intake, environmental conditions, and the way of feeding and management will also have a significant impact on the immunity of dairy cows. For example, during the peripartum period, the zinc level in the body of dairy cows often drops to a critical low point, approaching a deficient state.

[0004] Moreover, during the peripartum period, dairy cows are prone to a series of health problems, such as milk fever, mastitis, fatty liver, and ketosis, etc. These diseases are all directly or indirectly related to the decline in immunity. Therefore, a feed additive for improving the immunity of dairy cows during the peripartum period is proposed.

[0005] The above information disclosed in this background art is only used to increase the understanding of the background art of the present invention. Therefore, it may include prior art that is not known to those of ordinary skill in the art. Summary of the Invention

[0006] The present invention aims to solve at least one of the technical problems existing in the prior art. For this reason, an object of the present invention is to propose a feed additive for improving the immunity of dairy cows during the peripartum period.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A feed additive for improving the immunity of dairy cows during the peripartum period, comprising the following components:

[0009] Nutritional additives: vitamin E: 500 - 1000 IU / kg, selenium: 0.1 - 0.3 mg / kg, zinc: 30 - 50 mg / kg, copper: 10 - 20 mg / kg, manganese: 20 - 40 mg / kg of feed, calcium: 0.95 - 1.1 mmol / L, linseed oil: 10 - 20 mL / kg;

[0010] Non-nutritive additives: Lactic acid bacteria: 1 - 5 x 10^9 CFU / kg, prebiotics: 5 - 10 g / kg, rosemary extract: 50 - 100 mg / kg, green tea extract: 50 - 100 mg / kg, yeast extract: 50 - 100 mg / kg.

[0011] As a further optimized solution of the present invention, the prebiotic is one of fructooligosaccharide, inulin, and pectin, the probiotic is one of freeze-dried powders of Lactobacillus acidophilus or Lactobacillus plantarum, and the lactic acid bacteria is one of Lactobacillus acidophilus and Lactobacillus plantarum.

[0012] As a further optimized solution of the present invention, the rosemary extract is a water-soluble rosemary extract, the green tea extract is a green tea extract of epigallocatechin gallate, and the yeast extract is a yeast extract rich in β-glucan.

[0013] As a further optimized solution of the present invention, the linseed oil is cold-pressed linseed oil, the calcium is one of calcium carbonate and calcium chloride, the zinc is zinc sulfate, the copper is copper sulfate, the manganese is manganese sulfate, the selenium is selenium yeast, and the vitamin E is in powder or liquid state.

[0014] Preparation method of a feed additive for improving the immunity of dairy cows during the peripartum period:

[0015] Step 1: Pretreat vitamin E, selenium, zinc, copper, manganese, and calcium respectively.

[0016] Step 2: Use a high-speed mixer to uniformly mix the pretreated vitamin E, selenium, zinc, copper, manganese, and calcium with the prebiotic, and add a lubricant during the mixing process.

[0017] Step 3: Mix the mixed powder with linseed oil and use a wet grinder to grind it so that the linseed oil is uniformly distributed in the powder and forms tiny oil droplets.

[0018] Step 4: Spray-dry the ground mixture to remove moisture and form a dry powder.

[0019] Step 5: Add the freeze-dried probiotic powder, rosemary extract, and green tea extract to the spray-dried powder and use a fluidized bed mixer to mix them so that the probiotics and antioxidants are uniformly distributed and their activity loss is reduced.

[0020] Step 6: Add the yeast extract powder rich in β-glucan to the mixture and use a fluidized bed mixer to mix it so that the yeast extract is uniformly distributed.

[0021] Step 7: Microencapsulate the mixed feed additive.

[0022] As a further optimization scheme of the present invention, in step two, the lubricant is calcium stearate.

[0023] As a further optimization scheme of the present invention, the mixing time using a high-speed mixer is 5 - 15 min, and the rotation speed is 500 - 1500 rpm; in step three, the grinding time using a wet grinder is 10 - 30 min, and the rotation speed is 1000 - 3000 rpm; in step four, the spray drying time is 30 - 60 min, and the rotation speed is 10000 - 40000 rpm; in step five, the mixing time using a fluidized bed mixer is 5 - 10 min, and the rotation speed is 20 - 60 rpm; in step six, the mixing time is 5 - 10 min, and the rotation speed is 20 - 60 rpm.

[0024] Compared with the prior art, the beneficial effects of the present invention are:

[0025] In the present invention, nutrients such as vitamin E, selenium, zinc, copper, and manganese in the nutritional additive can enhance the antioxidant capacity and immune function of dairy cows, thereby preventing the occurrence of various diseases, including oxidative stress-related diseases, infectious diseases, reproductive disorders, skeletal diseases, etc. And by adding calcium and adjusting the dietary energy level, the occurrence of metabolic diseases such as hypocalcemia and ketosis can be prevented. Lactic acid bacteria and prebiotics can regulate the balance of intestinal flora and prevent the occurrence of digestive system diseases. By improving the immunity of dairy cows, reducing the occurrence of diseases, ensuring the health status of dairy cows, thereby increasing milk production, increasing the income of the ranch, and improving the economic benefits of the ranch.

[0026] The above summary is only for the purpose of the specification and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of the present invention will be readily apparent by reference to the drawings and the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a flowchart of the preparation method of the feed additive of the present invention;

[0028] Figure 2 It is a flowchart of step seven in the preparation method of the feed additive of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0029] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0030] Example 1

[0031] As shown in the attached Figure 1 and attached Figure 2 shown, a feed additive for improving the immunity of dairy cows during the peripartum period, comprising the following components:

[0032] Nutritional additives: Vitamin E: 500 IU / kg, Selenium: 0.1 mg / kg, Zinc: 30 mg / kg, Copper: 10 mg / kg, Manganese: 20 mg / kg of feed, Calcium: 0.95 mmol / L, Flaxseed oil: 10 mL / kg;

[0033] Non-nutritional additives: Lactobacillus: 1x10^9 CFU / kg, Prebiotic: 5 g / kg, Rosemary extract: 50 mg / kg, Green tea extract: 50 mg / kg, Yeast extract: 50 mg / kg.

[0034] Specifically, the prebiotic is fructooligosaccharide, the probiotic is freeze-dried powder of Lactobacillus acidophilus, and the lactobacillus is Lactobacillus acidophilus.

[0035] Specifically, the rosemary extract is a water-soluble rosemary extract, the green tea extract is the green tea extract in epigallocatechin gallate, and the yeast extract is a yeast extract rich in β-glucan.

[0036] Specifically, the flaxseed oil is cold-pressed flaxseed oil, the calcium is calcium carbonate, the zinc is zinc sulfate, the copper is copper sulfate, the manganese is manganese sulfate, the selenium is selenium yeast, and the vitamin E is in powder form.

[0037] Preparation method of the feed additive for improving the immunity of dairy cows during the peripartum period:

[0038] Step 1: Pretreat vitamin E, selenium, zinc, copper, manganese and calcium respectively;

[0039] Specifically, microencapsulate vitamin E:

[0040] Use gum arabic as the carrier material for microencapsulating vitamin E. Mix vitamin E with gum arabic, add Tween 80, and emulsify in a high-speed shear emulsifier to form a water-in-oil emulsion.

[0041] Spray-dry the emulsified mixture through a spray dryer to form microencapsulated vitamin E powder.

[0042] Microencapsulate selenium yeast:

[0043] Mix selenium yeast with polyvinylpyrrolidone PVP. Use the complex coacervation method to mix selenium yeast with sodium alginate to form microcapsules. Cure the microcapsules with glutaraldehyde, and then, centrifuge to separate the microencapsulated selenium yeast.

[0044] Specific steps for pre-treating zinc, copper, and manganese:

[0045] Dissolve zinc sulfate, copper sulfate, and manganese sulfate in deionized water respectively, add citric acid to form stable chelates with metal ions, and make the chelated solution into powder by spray drying.

[0046] Specific steps for pre-treating calcium:

[0047] Dissolve calcium carbonate or calcium chloride in deionized water, add citric acid to prevent adverse reactions of calcium ions with other components in the feed, and treat the dissolved and stabilized calcium solution by microencapsulation technology.

[0048] Step 2: Use a high-speed mixer to mix the pre-treated vitamin E, selenium, zinc, copper, manganese, and calcium with prebiotics evenly. The mixing time is 5 min and the rotation speed is 500 rpm. During the mixing process, add calcium stearate;

[0049] Step 3: Mix the mixed powder with linseed oil and grind it using a wet grinder. The grinding time is 10 min and the rotation speed is 1000 rpm, so that the linseed oil is evenly distributed in the powder and forms tiny oil droplets;

[0050] Step 4: Spray dry the ground mixture to remove moisture and form dry powder. The spray drying time is 30 min and the rotation speed is 10000 rpm;

[0051] Step 5: Add freeze-dried probiotic powder, rosemary extract, and green tea extract to the spray-dried powder and mix them using a fluidized bed mixer. The mixing time is 5 min and the rotation speed is 20 rpm, so that the probiotics and antioxidants are evenly distributed and their activity loss is reduced;

[0052] Step 6: Add yeast extract powder rich in β-glucan to the mixture, mix for 5 min at a rotation speed of 20 rpm, and use a fluidized bed mixer to mix it evenly;

[0053] Step 7: Microencapsulate the mixed feed additive.

[0054] Specifically, use gum arabic as the wall material, dissolve it in deionized water, and add Tween 80 and citric acid.

[0055] Mix the mixed feed additive with the wall material solution at a ratio of 3:40, and use a high-speed shear emulsifier to perform preliminary emulsification at 2000 rpm for 5 min to form a preliminary emulsification system.

[0056] Pump the emulsified mixture into a spray dryer, set the feeding rate at 50 L / h, the inlet air temperature at 60 °C, the outlet air temperature at 40 °C, and the spray pressure at 0.1 MPa.

[0057] Collect the microencapsulated feed additive through a cyclone separator, remove oversized or undersized particles from the microencapsulated feed additive by screening. Finally, store the microencapsulated feed additive.

[0058] Example 2

[0059] A feed additive for improving the immunity of dairy cows during the peripartum period, comprising the following components:

[0060] Nutritional additives: Vitamin E: 700 IU / kg, Selenium: 0.2 mg / kg, Zinc: 40 mg / kg, Copper: 15 mg / kg, Manganese: 30 mg / kg feed, Calcium: 1 mmol / L, Flaxseed oil: 15 mL / kg;

[0061] Non-nutritional additives: Lactobacillus: 3x10^9 CFU / kg, Prebiotic: 7 g / kg, Rosemary extract: 70 mg / kg, Green tea extract: 80 mg / kg, Yeast extract: 75 mg / kg.

[0062] Specifically, the prebiotic is inulin, the probiotic is the freeze-dried powder of Lactobacillus plantarum, and the lactic acid bacteria is Lactobacillus plantarum.

[0063] Specifically, the rosemary extract is a water-soluble rosemary extract, the green tea extract is the green tea extract in epigallocatechin gallate, and the yeast extract is a yeast extract rich in β-glucan.

[0064] Specifically, the flaxseed oil is cold-pressed flaxseed oil, the calcium is calcium chloride, the zinc is zinc sulfate, the copper is copper sulfate, the manganese is manganese sulfate, the selenium is selenium yeast, and the vitamin E is in powder form.

[0065] Preparation method of a feed additive for improving the immunity of dairy cows during the peripartum period:

[0066] Step 1: Pretreat vitamin E, selenium, zinc, copper, manganese, and calcium respectively;

[0067] Step 2: Use a high-speed mixer to mix the pretreated vitamin E, selenium, zinc, copper, manganese, and calcium with the prebiotic evenly. The mixing time is 10 min, and the rotation speed is 1000 rpm. During the mixing process, add calcium stearate;

[0068] Step 3: Mix the mixed powder with flaxseed oil and grind it using a wet grinder. The grinding time is 20 min, and the rotation speed is 2000 rpm, so that the flaxseed oil is evenly distributed in the powder and forms tiny oil droplets;

[0069] Step 4: Spray-dry the ground mixture to remove moisture and form a dry powder. The spray-drying time is 45 min and the rotation speed is 30,000 rpm.

[0070] Step 5: Add freeze-dried probiotic powder, rosemary extract, and green tea extract to the spray-dried powder and mix using a fluidized bed mixer for 7 min at a rotation speed of 40 rpm to evenly distribute the probiotics and antioxidants and reduce their loss of activity.

[0071] Step 6: Add the yeast extract powder rich in β-glucan to the mixture and mix for 7 min at a rotation speed of 40 rpm using a fluidized bed mixer to evenly distribute the yeast extract.

[0072] Step 7: Microencapsulate the mixed feed additive.

[0073] Specifically, use gum arabic as the wall material, dissolve it in deionized water, and add Tween 80 and citric acid.

[0074] Mix the mixed feed additive with the wall material solution at a ratio of 3:40 and perform preliminary emulsification using a high-speed shear emulsifier at 2500 rpm for 7 min to form a preliminary emulsification system.

[0075] Pump the emulsified mixture into a spray dryer, set the feed rate to 100 L / h, the inlet air temperature to 75 °C, the outlet air temperature to 45 °C, and the spray pressure to 0.1 MPa.

[0076] Collect the microencapsulated feed additive through a cyclone separator, remove oversized or undersized particles from the microencapsulated feed additive by screening, and finally store the microencapsulated feed additive.

[0077] Example 3

[0078] A feed additive for improving the immunity of dairy cows during the peripartum period, comprising the following components:

[0079] Nutritional additives: Vitamin E: 1000 IU / kg, Selenium: 0.3 mg / kg, Zinc: 50 mg / kg, Copper: 20 mg / kg, Manganese: 40 mg / kg feed, Calcium: 1.1 mmol / L, Linseed oil: 20 mL / kg;

[0080] Non-nutritional additives: Lactic acid bacteria: 5x10^9 CFU / kg, Prebiotics: 10 g / kg, Rosemary extract: 100 mg / kg, Green tea extract: 100 mg / kg, Yeast extract: 100 mg / kg.

[0081] Specifically, the prebiotic is pectin, the probiotic is freeze-dried powder of Lactobacillus acidophilus, and the lactic acid bacterium is Lactobacillus acidophilus.

[0082] Specifically, the rosemary extract is water-soluble rosemary extract, the green tea extract is the green tea extract in epigallocatechin gallate, and the yeast extract is the yeast extract rich in β-glucan.

[0083] Specifically, the linseed oil is cold-pressed linseed oil, the calcium is calcium carbonate, the zinc is zinc sulfate, the copper is copper sulfate, the manganese is manganese sulfate, the selenium is selenium yeast, and the vitamin E is in liquid state.

[0084] Preparation method of a feed additive for improving the immunity of dairy cows during the peripartum period:

[0085] Step 1: Pretreat vitamin E, selenium, zinc, copper, manganese, and calcium respectively.

[0086] Step 2: Use a high-speed mixer to uniformly mix the pretreated vitamin E, selenium, zinc, copper, manganese, and calcium with the prebiotic. The mixing time is 15 min, and the rotation speed is 1500 rpm. During the mixing process, add calcium stearate.

[0087] Step 3: Mix the mixed powder with linseed oil and grind it using a wet grinder. The grinding time is 30 min, and the rotation speed is 3000 rpm, so that the linseed oil is uniformly distributed in the powder and forms tiny oil droplets.

[0088] Step 4: Spray-dry the ground mixture to remove moisture and form a dry powder. The spray-drying time is 60 min, and the rotation speed is 40000 rpm.

[0089] Step 5: Add the freeze-dried probiotic powder, rosemary extract, and green tea extract to the spray-dried powder and mix them using a fluidized bed mixer. The mixing time is 10 min, and the rotation speed is 60 rpm, so that the probiotics and antioxidants are uniformly distributed and their activity loss is reduced.

[0090] Step 6: Add the yeast extract powder rich in β-glucan to the mixture, mix for 10 min at a rotation speed of 60 rpm, and use a fluidized bed mixer to mix it so that the yeast extract is uniformly distributed.

[0091] Step 7: Microencapsulate the mixed feed additive.

[0092] Specifically, use arabic gum as the wall material, dissolve it in deionized water, and add Tween 80 and citric acid.

[0093] Mix the prepared feed additive and the wall material solution in a ratio of 3:40, and perform preliminary emulsification using a high-speed shear emulsifier at 3000 rpm for 10 min to form a preliminary emulsification system.

[0094] Pump the emulsified mixture into a spray dryer, set the feed rate at 200 L / h, the inlet air temperature at 80 °C, the outlet air temperature at 50 °C, and the spray pressure at 0.1 MPa.

[0095] Collect the microencapsulated feed additive through a cyclone separator, remove oversized or undersized particles from the microencapsulated feed additive by screening. Finally, store the microencapsulated feed additive.

[0096] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "example", "specific example" or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0097] Parts not involved in the present invention are the same as the prior art or can be implemented using the prior art. Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A feed additive for improving the peripartum immunity of dairy cows, characterized in that: Includes the following ingredients: Nutritional additives: Vitamin E: 500-1000IU / kg, selenium: 0.1-0.3mg / kg, zinc: 30-50mg / kg, copper: 10-20mg / kg, manganese: 20-40mg / kg feed, calcium: 0.95-1.1mmol / L, linseed oil: 10-20mL / kg; Non-nutritional additives: lactic acid bacteria: 1-5x10^9 CFU / kg, prebiotics: 5-10g / kg, rosemary extract: 50-100mg / kg, green tea extract: 50-100mg / kg, yeast extract: 50-100mg / kg.

2. A feed additive for improving the peripartum immunity of dairy cows according to claim 1, characterized in that: The prebiotic is one of oligofructose, inulin and pectin, the probiotic is one of freeze-dried powder of Lactobacillus acidophilus or Lactobacillus plantarum, and the lactic acid bacteria is one of Lactobacillus acidophilus and Lactobacillus plantarum.

3. A feed additive for improving the peripartum immunity of dairy cows according to claim 1, characterized in that: The rosemary extract is a water-soluble rosemary extract, the green tea extract is a green tea extract in epigallocatechin gallate, and the yeast extract is a yeast extract rich in beta-glucan.

4. A feed additive for improving the peripartum immunity of dairy cows according to claim 1, characterized in that: The flaxseed oil is cold-pressed flaxseed oil, the calcium is one of calcium carbonate or calcium chloride, the zinc is zinc sulfate, the copper is copper sulfate, the manganese is manganese sulfate, the selenium is selenium yeast, and the vitamin E is in powder or liquid state.

5. A feed additive for improving the peripartum immunity of dairy cows according to any one of claims 1 to 4, characterized in that: Prepared by the following method: Step 1: pre-treating vitamin E, selenium, zinc, copper, manganese and calcium respectively; Step 2: Use a high-speed mixer to evenly mix the pretreated vitamin E, selenium, zinc, copper, manganese and calcium with the prebiotics, and add a lubricant during the mixing process; Step 3: Mix the mixed powder with linseed oil and grind it using a wet grinder so that the linseed oil is evenly distributed in the powder and forms tiny oil droplets; Step 4: spray drying the ground mixture to remove moisture and form a dry powder; Step 5: adding freeze-dried probiotic powder, rosemary extract and green tea extract to the spray-dried powder, and mixing using a fluidized bed mixer to uniformly distribute the probiotics and antioxidants; Step 6: adding β-glucan-rich yeast extract powder to the mixture and mixing using a fluidized bed mixer to ensure uniform distribution of the yeast extract; Step 7: Microencapsulate the mixed feed additives.

6. A feed additive for improving the peripartum immunity of dairy cows according to claim 5, characterized in that: In step 2, the lubricant is calcium stearate.

7. The feed additive for improving the peripartum immunity of dairy cows according to claim 5, characterized in that: In step 2, a high-speed mixer is used for mixing for 5-15 minutes at a rotation speed of 500-1500 rpm; in step 3, a wet grinder is used for grinding for 10-30 minutes at a rotation speed of 1000-3000 rpm; in step 4, the spray drying time is 30-60 minutes at a rotation speed of 10000-40000 rpm; in step 5, a fluidized bed mixer is used for mixing for 5-10 minutes at a rotation speed of 20-60 rpm; in step 6, the mixing time is 5-10 minutes at a rotation speed of 20-60 rpm.

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