Intestinal health-care composition containing sonchus brachyotus polysaccharide and preparation method of intestinal health-care composition

Abstract

The invention belongs to the technical field of health-care food, and particularly relates to an intestinal tract health-care composition containing sonchus brachyotus polysaccharide, which is prepared from the following components in parts by mass: 20 to 60 parts of sonchus brachyotus polysaccharide, 10 to 20 parts of endothelium corneum gigeriae galli and 5 to 10 parts of rutin. 1-5 parts of sodium alginate and 3-8 parts of starch. The composition provided by the invention can be used for preparing a pharmaceutical composition for intestinal health care or a food composition for intestinal health care.

Description

Technical Field

[0001] The present invention belongs to the technical field of health foods, and particularly relates to an intestinal health care composition containing Sonchus arvensis polysaccharide and a preparation method thereof. Background Art

[0002] Sonchus arvensis, also known as wild bitter vegetable, bitter fleabane, bitter kudzu, and curly dock, etc., belongs to the Compositae perennial herb, and the whole herb can be used as medicine. Currently, it is often used as a wild vegetable. Sonchus arvensis is rich in nutritional components, containing a variety of inorganic elements such as calcium, magnesium, manganese, titanium, selenium, and zinc, and has strong free radical scavenging and antioxidant abilities. Digestive system diseases seriously affect patients' study, work, and life, and the imbalance of the intestinal microbiota is related to inflammatory bowel disease. Many studies have shown that traditional Chinese medicine can achieve the purpose of treating diseases by interfering with the homeostasis of the human intestinal flora. At present, there are many studies on the application of traditional Chinese medicine compositions in the digestive system such as the intestine in the prior art, but there is no research on using Sonchus arvensis polysaccharide as the main component for intestinal health care. Summary of the Invention

[0003] The present invention mainly provides an intestinal health care composition containing Sonchus arvensis polysaccharide, and the specific scheme is as follows:

[0004] An intestinal health care composition containing Sonchus arvensis polysaccharide, calculated by mass, comprises the following components: 20 - 60 parts of Sonchus arvensis polysaccharide, 10 - 20 parts of chicken gizzard-membrane, 5 - 10 parts of rutin; 1 - 5 parts of sodium alginate and 3 - 8 parts of starch.

[0005] Further, calculated by mass, it comprises the following components: 30 - 40 parts of Sonchus arvensis polysaccharide, 13 - 18 parts of chicken gizzard-membrane, 5 - 10 parts of rutin; 3 - 5 parts of sodium alginate and 4 - 6 parts of starch.

[0006] Further, the preparation of the Sonchus arvensis polysaccharide comprises the following steps:

[0007] a. Dry and crush Sonchus arvensis to obtain Sonchus arvensis powder; place Sonchus arvensis polysaccharide powder in water to prepare a dispersion liquid with a concentration of 8 - 12 g / L; at 55 °C, add cellulase and papain to the dispersion liquid for enzymatic hydrolysis for 0.5 - 1.5 h, and then inactivate the enzyme by boiling water bath, and obtain crude polysaccharide after water extraction;

[0008] b. Prepare a protein removal reagent for protein removal; place the crude polysaccharide in water, stir evenly to obtain a crude polysaccharide solution with a concentration of 3 - 8 g / L; mix the polysaccharide solution with the protein removal reagent, stir evenly, and then let it stand for stratification; take the upper layer of the polysaccharide solution and repeat the protein removal step until the middle layer is clear; dry the solution in the upper layer to obtain Sonchus arvensis polysaccharide.

[0009] Further, the mass ratio of papain to pectinase is 1:1.5 - 2; the water extraction is carried out with 95 °C water for 1.5 - 2.5 h.

[0010] Furthermore, the protein-depleting reagent is obtained by mixing chloroform and n-butanol in a volume ratio of 4:1; the volume ratio of the protein-depleting reagent to the crude polysaccharide solution is 1:3-5.

[0011] Furthermore, after removing the lower-layer protein-depleting reagent, the remaining middle layer and upper layer are centrifuged to obtain the upper-layer polysaccharide solution.

[0012] A preparation method of the above-mentioned intestinal health care composition containing Sonchus brachyotus polysaccharide comprises the following steps:

[0013] (1) Weigh the raw materials according to the formula; place the raw materials in water and mix them evenly to obtain a precursor.

[0014] (2) Pour the precursor into a mold and add a cross-linking agent; after cross-linking is completed, take it out to obtain the intestinal health care composition.

[0015] Furthermore, the cross-linking agent is calcium chloride; the mass ratio of the cross-linking agent to sodium alginate is 1:10-30.

[0016] Furthermore, after adding the cross-linking agent, homogenize it, and let it stand for 8-12 h after homogenization is completed.

[0017] An application of the above-mentioned intestinal health care composition containing Sonchus brachyotus polysaccharide in preparing a pharmaceutical composition for intestinal health care or in preparing a food composition for intestinal health care.

[0018] Adopting the above scheme, the method of the present invention has the following advantages:

[0019] The intestinal health care composition containing Sonchus brachyotus polysaccharide of the present invention has good free radical scavenging ability, can reduce the damage of free radicals to the intestine, protect the barrier function of the gastrointestinal tract, inhibit the growth of harmful bacteria, help digestion, regulate the stomach and intestines, regulate and improve the gastrointestinal flora, protect the gastrointestinal mucosa, and prevent mucosal ulcers.

[0020] The present invention broadens the application field of Sonchus brachyotus polysaccharide and provides a new choice for gastrointestinal drugs and foods. Description of the Drawings

[0021] Figure 1 is the polysaccharide preparation flow chart of the present invention;

[0022] Figure 2 is the ABTS free radical scavenging comparison chart of each example of the present invention. Detailed Embodiments

[0023] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0024] Example 1 of polysaccharide extraction:

[0025] (1) Dry and crush Sonchus brachyotus DC. to obtain Sonchus brachyotus DC. powder; place Sonchus brachyotus DC. polysaccharide powder in water to prepare a dispersion with a concentration of 10 g / L; at 55 °C, add cellulase and papain with a mass ratio of 1:1.5 to the dispersion and enzymatically hydrolyze for 1 h, then inactivate the enzyme in a boiling water bath for 20 min, and extract with 95 °C water for 2 h to obtain crude polysaccharide;

[0026] (2) Prepare a deproteinization reagent by mixing chloroform and n-butanol in a volume ratio of 4:1 for deproteinization; place the crude polysaccharide in water, stir evenly to obtain a 5 g / L crude polysaccharide solution; mix the polysaccharide solution and the deproteinization reagent in a volume ratio of 1:4, mix evenly, and then let it stand for stratification; remove the lower-layer deproteinization reagent, centrifuge the remaining middle layer and upper layer to obtain the upper-layer polysaccharide solution; take the upper-layer polysaccharide solution and repeat the deproteinization step until the middle layer is clear; dry the upper-layer solution to obtain Sonchus brachyotus DC. polysaccharide.

[0027] Example 2 of polysaccharide extraction:

[0028] (1) Dry and crush Sonchus brachyotus DC. to obtain Sonchus brachyotus DC. powder; place Sonchus brachyotus DC. polysaccharide powder in water to prepare a dispersion with a concentration of 8 g / L; at 55 °C, add cellulase and papain with a mass ratio of 1:1.5 to the dispersion and enzymatically hydrolyze for 1 h, then inactivate the enzyme in a boiling water bath for 20 min, and extract with 95 °C water for 2 h to obtain crude polysaccharide;

[0029] (2) Prepare a deproteinization reagent by mixing chloroform and n-butanol in a volume ratio of 4:1 for deproteinization; place the crude polysaccharide in water, stir evenly to obtain a 5 g / L crude polysaccharide solution; mix the polysaccharide solution and the deproteinization reagent in a volume ratio of 1:4, mix evenly, and then let it stand for stratification; remove the lower-layer deproteinization reagent, centrifuge the remaining middle layer and upper layer to obtain the upper-layer polysaccharide solution; take the upper-layer polysaccharide solution and repeat the deproteinization step until the middle layer is clear; dry the upper-layer solution to obtain Sonchus brachyotus DC. polysaccharide.

[0030] Example 3 of polysaccharide extraction:

[0031] (1) Dry and crush Sonchus arvensis L. to obtain Sonchus arvensis L. powder; take Sonchus arvensis L. polysaccharide powder and prepare a dispersion with a concentration of 12 g / L in water; at 55 °C, add cellulase and papain with a mass ratio of 1:1.5 to the dispersion and enzymatically hydrolyze for 1.5 h, then inactivate the enzyme by boiling water bath for 20 min, and extract with 95 °C water for 2 h to obtain crude polysaccharide;

[0032] (2) Prepare a deproteinization reagent by mixing chloroform and n-butanol in a volume ratio of 4:1 for deproteinization; place the crude polysaccharide in water, stir evenly to obtain a 5 g / L crude polysaccharide solution; mix the polysaccharide solution and the deproteinization reagent in a volume ratio of 1:4, mix evenly, and then let it stand for stratification; after removing the lower-layer deproteinization reagent, centrifuge the remaining middle layer and upper layer to obtain the upper-layer polysaccharide solution; take the upper-layer polysaccharide solution and repeat the deproteinization step until the middle layer is clear; dry the upper-layer solution to obtain Sonchus arvensis L. polysaccharide.

[0033] Example 4 of polysaccharide extraction:

[0034] (1) Dry and crush Sonchus arvensis L. to obtain Sonchus arvensis L. powder; take Sonchus arvensis L. polysaccharide powder and prepare a dispersion with a concentration of 10 g / L in water; at 55 °C, add cellulase and papain with a mass ratio of 1:2 to the dispersion and enzymatically hydrolyze for 0.5 h, then inactivate the enzyme by boiling water bath for 20 min, and extract with 95 °C water for 2 h to obtain crude polysaccharide;

[0035] (2) Prepare a deproteinization reagent by mixing chloroform and n-butanol in a volume ratio of 4:1 for deproteinization; place the crude polysaccharide in water, stir evenly to obtain a 5 g / L crude polysaccharide solution; mix the polysaccharide solution and the deproteinization reagent in a volume ratio of 1:3 - 5, mix evenly, and then let it stand for stratification; after removing the lower-layer deproteinization reagent, centrifuge the remaining middle layer and upper layer to obtain the upper-layer polysaccharide solution; take the upper-layer polysaccharide solution and repeat the deproteinization step until the middle layer is clear; dry the upper-layer solution to obtain Sonchus arvensis L. polysaccharide.

[0036] Example 5 of polysaccharide extraction:

[0037] (1) Dry and crush Sonchus arvensis L. to obtain Sonchus arvensis L. powder; take Sonchus arvensis L. polysaccharide powder and prepare a dispersion with a concentration of 10 g / L in water; at 55 °C, add cellulase and papain with a mass ratio of 1:1.5 to the dispersion and enzymatically hydrolyze for 1 h, then inactivate the enzyme by boiling water bath for 20 min, and extract with 95 °C water for 2 h to obtain crude polysaccharide;

[0038] (2) Use a deproteinization reagent prepared by mixing chloroform and n-butanol in a volume ratio of 4:1 for deproteinization; place the crude polysaccharide in water, stir evenly to obtain a crude polysaccharide solution with a concentration of 8 g / L; mix the polysaccharide solution and the deproteinization reagent in a volume ratio of 1:3, stir evenly, and then let it stand for stratification; after removing the lower-layer deproteinization reagent, centrifuge the remaining middle and upper layers to obtain the upper-layer polysaccharide solution; take the upper-layer polysaccharide solution and repeat the deproteinization step until the middle layer is clear; dry the upper-layer solution to obtain Sonchus brachyotus polysaccharide.

[0039] Example 1:

[0040] (1) By weight, take 35 parts of the Sonchus brachyotus polysaccharide obtained in Example 1, 15 parts of chicken gizzard-membrane, and 8 parts of rutin; place 3 parts of sodium alginate and 5 parts of starch in water and mix evenly to obtain a precursor.

[0041] (2) Pour the precursor into a mold, add 0.15 part of calcium chloride and then homogenize. After homogenization, let it stand for 10 h; after crosslinking is completed, take it out to obtain an intestinal health care composition.

[0042] Example 2:

[0043] (1) By weight, take 20 parts of the Sonchus brachyotus polysaccharide obtained in Example 1, 15 parts of chicken gizzard-membrane, and 8 parts of rutin; place 3 parts of sodium alginate and 5 parts of starch in water and mix evenly to obtain a precursor.

[0044] (2) Pour the precursor into a mold, add 0.15 part of calcium chloride and then homogenize. After homogenization, let it stand for 10 h; after crosslinking is completed, take it out to obtain an intestinal health care composition.

[0045] Example 3:

[0046] (1) By weight, take 60 parts of the Sonchus brachyotus polysaccharide obtained in Example 1, 15 parts of chicken gizzard-membrane, and 8 parts of rutin; place 3 parts of sodium alginate and 5 parts of starch in water and mix evenly to obtain a precursor.

[0047] (2) Pour the precursor into a mold, add 0.15 part of calcium chloride and then homogenize. After homogenization, let it stand for 10 h; after crosslinking is completed, take it out to obtain an intestinal health care composition.

[0048] Example 4:

[0049] (1) By weight, take 35 parts of the Sonchus brachyotus polysaccharide obtained in Example 1, 15 parts of chicken gizzard-membrane, and 8 parts of rutin; place 1 part of sodium alginate and 5 parts of starch in water and mix evenly to obtain a precursor.

[0050] (2) Pour the precursor into a mold, add 0.15 part of calcium chloride and then homogenize. After homogenization, let it stand for 12 h; after crosslinking is completed, take it out to obtain an intestinal health care composition.

[0051] Example test: Take 8 mL of 7 mmol / L ABTS and mix it with 141 μL of 140 mmol / L potassium persulfate solution, and react in the dark at 4°C for 12 h to obtain an ABTS radical stock solution. Dilute the stock solution with 10 mmol / L phosphate buffer solution with pH = 7.4 to OD = 0.7 for standby. Take 1 g of the intestinal health care composition of each example, crush it and place it in water, add 3 mL of ABTS radical solution, react in the dark for 1 h, and measure the absorbance value Ai at 734 nm. Use 1 mL of pure water to replace the sample, and measure the absorbance value AC under the same conditions; use 3 mL of pure water to replace the ABTS radical solution as the sample control group and measure the absorbance value Aj under the same conditions. Calculate the scavenging rate according to the formula [1 - (Ai - Aj) / Ac] × 100.

[0052]

[0053]

[0054] As can be seen from the above table, the intestinal health care compositions of the present invention all show obvious antioxidant activity, and are positively correlated with the content of Sonchus brachyotus polysaccharide. The intestinal health care composition of Example 3 with a high content of Sonchus brachyotus polysaccharide has better antioxidant activity against ABTS radicals.

[0055] For those skilled in the art, various corresponding changes and deformations can be made according to the technical solutions and concepts described above, and all these changes and deformations should fall within the protection scope of the claims of the present invention.

Claims

1. An intestinal health care composition containing Sonchus oleraceus polysaccharide, characterized in that: The invention comprises the following components by weight: 20-60 parts of sonchus endive polysaccharide, 10-20 parts of chicken gizzard lining, 5-10 parts of rutin, 1-5 parts of sodium alginate and 3-8 parts of starch.

2. The intestinal health care composition containing Sonchus oleraceus polysaccharide according to claim 1, characterized in that: The invention comprises the following components by weight: 30-40 parts of sonchus endive polysaccharide, 13-18 parts of chicken gizzard lining, 5-10 parts of rutin, 3-5 parts of sodium alginate and 4-6 parts of starch.

3. The intestinal health care composition containing Sonchus oleraceus polysaccharide according to claim 1, characterized in that: The preparation of the Sonchus oleraceus polysaccharide comprises the following steps: a. Dry and crush Sonchus endive to obtain Sonchus endive powder; take Sonchus endive polysaccharide powder and place it in water to prepare a dispersion of 8 to 12 g / L; add cellulase and papain to the dispersion at 55°C for 0.5 to 1.5 hours, then inactivate the enzyme in a boiling water bath, and obtain crude polysaccharide after water extraction; b. Prepare a deproteinizing agent for deproteinization; place the crude polysaccharide in water, stir evenly to obtain a 3-8 g / L crude polysaccharide solution; mix the polysaccharide solution with the deproteinizing agent, mix evenly, and let stand to separate layers; take the upper layer of polysaccharide solution, repeat the deproteinization steps until the middle layer is clear; dry the upper layer solution to obtain Sonchus oleraceus polysaccharide.

4. The intestinal health care composition containing Sonchus oleraceus polysaccharide according to claim 1, characterized in that: The mass ratio of papain to pectinase is 1:1.5-2; and the water extraction is performed by using 95°C water for 1.5-2.5 hours.

5. The intestinal health care composition containing Sonchus oleraceus polysaccharide according to claim 1, characterized in that: The deproteinizing reagent is obtained by mixing chloroform and n-butanol in a volume ratio of 4:1; the volume ratio of the deproteinizing reagent to the crude polysaccharide solution is 1:3-5.

6. The intestinal health care composition containing Sonchus oleraceus polysaccharide according to claim 1, characterized in that: After the deproteinization reagent in the lower layer is removed, the remaining middle layer and upper layer are centrifuged to obtain the polysaccharide solution in the upper layer.

7. A method for preparing the intestinal health care composition containing Sonchus oleraceus polysaccharide according to claim 1 or 7, characterized in that: The following steps are involved: (1) Weighing raw materials according to the formula; placing the raw materials in water and mixing them evenly to obtain a precursor; (2) pouring the precursor into a mold and adding a crosslinking agent; taking it out after crosslinking is completed to obtain an intestinal health care composition.

8. The method for preparing the intestinal health care composition containing Sonchus oleraceus polysaccharide according to claim 7, characterized in that: The cross-linking agent is calcium chloride; the mass ratio of the cross-linking agent to sodium alginate is 1:10-30.

9. The method for preparing the intestinal health care composition containing Sonchus oleraceus polysaccharide according to claim 7, characterized in that: After adding the cross-linking agent, homogenize and let stand for 8 to 12 hours.

10. Use of the intestinal health care composition containing Sonchus oleraceus polysaccharide according to claim 1 or 2 in preparing an intestinal health care pharmaceutical composition or in preparing an intestinal health care food composition.

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