Tightening long-acting anti-aging composition as well as preparation method and application thereof

Abstract

The invention discloses a compact long-acting anti-aging composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics, the composition comprises a chelating agent, a thickening agent, a humectant, sodium hyaluronate, a compound, a pH regulator, an uncaria extract, jellyfish peptide, acetyl hydroxyproline, an emulsifier, sodium polyaspartate, silymarin, a functional agent and water. According to the present invention, a variety of anti-aging active components are combined, such that the effects of skin elasticity improving, skin compactness improving and wrinkle reducing are synergistically provided, the skin barrier function is enhanced, the irritation on sensitive skin is avoided, and the anti-aging composition is especially suitable for sensitive skin use. Compared with the prior art, the composition disclosed by the invention shows remarkable effects in the aspects of aging resistance and skin barrier repair, and further enhances the stability and slow-release effect of the active ingredients by optimizing ingredient matching and a preparation process, so that a more lasting aging resistance effect and a better skin barrier protection function are realized.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly to a composition for firming and long-term anti-aging, a preparation method thereof, and an application thereof. Background Art

[0002] With the improvement of people's living standards and the increasing attention to their own images, skin anti-aging and barrier repair have become popular research directions in the cosmetics field. Skin aging is a complex physiological process, manifested as decreased skin elasticity, increased wrinkles, skin relaxation, etc. It not only affects appearance but may also lead to weakened skin barrier function, making the skin more vulnerable to external stimuli and damage. Therefore, developing a cosmetic composition that can effectively anti-aging and enhance skin barrier function has important market demand.

[0003] Currently, there are already some anti-aging cosmetics on the market, but most of them have certain limitations. For example, although some anti-aging products can improve skin elasticity, their effect on skin barrier repair is limited, and they may even cause discomfort to sensitive skin due to the presence of irritating ingredients; while some barrier repair products can enhance the skin's moisturizing property and ability to resist external stimuli, but their anti-aging effect is not significant. In addition, although there are some compositions in the prior art that attempt to achieve both anti-aging and barrier repair functions, their ingredient combinations and preparation processes are not optimized enough, resulting in the overall effect of the products not being ideal enough to meet consumers' demands for high-efficiency, mild, and multi-functional cosmetics.

[0004] For example, Chinese Patent CN116763683A discloses a skin anti-aging composition and its application. Its raw materials mainly include a solvent, a moisturizer, oxidized tannic acid, linoleic acid, a skin conditioner, an antioxidant, a thickener, a solubilizer, and a fragrance. By introducing oxidized tannic acid and linoleic acid and interacting with collagen, the invention improves the antioxidant activity and emulsification stability of the composition. However, the anti-aging and skin barrier effects of this composition still need to be improved, and there are deficiencies in its applicability to sensitive skin. Summary of the Invention

[0005] In order to solve the deficiencies in the prior art, the present invention aims to provide a composition for firming and long-term anti-aging, a preparation method thereof, and an application thereof. By optimizing the ingredient combination and preparation process, it realizes significant anti-aging effects and skin barrier repair functions, while avoiding irritation to sensitive skin and meeting consumers' demands for high-efficiency, mild, and multi-functional cosmetics.

[0006] To achieve the above-mentioned invention objectives, the present invention adopts the following technical solutions:

[0007] A composition for firming and long - lasting anti - aging, comprising the following components by weight percentage: chelating agent 0.01% - 0.03%, thickening agent 0.14% - 0.28%, humectant 1% - 3%, sodium hyaluronate 0.01% - 0.03%, complex 0.06% - 0.12%, pH regulator 0.1% - 0.2%, Uncaria rhynchophylla extract 0.2% - 0.4%, jellyfish peptide 0.02% - 0.06%, acetylhydroxyproline 0.2% - 0.6%, emulsifier 0.4% - 0.8%, sodium polyaspartate 0.2% - 0.6%, silymarin 0.1% - 0.3%, functional agent 0.2% - 0.4%, and the balance being water.

[0008] The chelating agent includes at least one of disodium EDTA and tetrasodium EDTA.

[0009] The thickening agent is at least one of carbomer, xanthan gum, and cetearyl alcohol.

[0010] The humectant is at least one of glycerol and propylene glycol.

[0011] The pH regulator is at least one of arginine and triethanolamine.

[0012] The emulsifier is at least one of hydrogenated lecithin, sodium stearoyl glutamate, cetearyl glucoside, and glyceryl stearate.

[0013] The complex is formed by mixing Euglena gracilis polysaccharide and hydrolyzed collagen in a mass ratio of 1:1 - 3.

[0014] The preparation method of the functional agent is as follows, by weight:

[0015] S1. Mix 2 - 4 parts of chitosan, 2 - 4 parts of fish collagen, and 15 - 25 parts of 20 - 35 wt% acetic acid aqueous solution to form a mixed solution. Add 10 - 20 parts of 1 - 3 wt% cross - linker aqueous solution to the mixed solution, stir evenly, and place the mixture at 35 - 45 °C for 10 - 30 hours to form a pretreatment.

[0016] S2. Mix the pretreatment obtained in step S1 with 0.5 - 1 part of enhancer and 3 - 5 parts of polycaprolactone, stir at 120 - 140 °C for 1 - 6 hours. Add 1 - 1.5 parts of hexafluoroisopropanol to the above mixture, stir evenly and then perform electrospinning. Electrospinning parameters: voltage 18 - 22 kV, spinning speed 0.05 - 0.2 mL / min, receiving plate distance 10 - 15 cm. Naturally air - dry the electrospun fibers, then soak them in a mixed solvent for annealing treatment. The mixed solvent is prepared by adding 0.8 - 1.2 parts of absolute ethanol and 4 - 6 parts of glycerol to 70 - 90 parts of water. After the annealing treatment is completed, air - dry the fibers and crush them to a particle size of 3 - 8 μm to finally obtain the functional agent.

[0017] The crosslinking agent is at least one of dialdehyde polyethyleneglycol, glyceraldehyde, bisbenzaldehyde polyethyleneglycol, and glucuronic acid.

[0018] Preferably, the crosslinking agent is glucuronic acid.

[0019] The enhancer is at least one of polyglycolic acid, trehalose, hyaluronic acid, and polyvinyl alcohol.

[0020] The preparation method of the composition for firming and long - lasting anti - aging is as follows:

[0021] Step 1: Add the chelating agent into water, heat to 80 - 95°C, then add part of the thickener into the water, stir until the thickener is completely dissolved to form a uniform solution. Add the remaining thickener and sodium hyaluronate into the solution, stir until completely dissolved, add the humectant, continue to stir until uniform. When the temperature drops to 50 - 70°C, add the complex and stir until completely dispersed to obtain a mixture.

[0022] Step 2: Lower the temperature of the mixture prepared in Step 1 to 45 - 55°C. Prepare a dilution of the pH regulator by adding it to water, add the dilution to the mixture, stir until uniform, continue to cool to 40 - 50°C, and sequentially add the Uncaria rhynchophylla extract, jellyfish peptide, and acetylhydroxyproline, stir until uniform to obtain a functional liquid.

[0023] Step 3: Lower the temperature of the functional liquid prepared in Step 2 to 30 - 45°C. Add the emulsifier, sodium polyaspartate, silymarin, and functional agent to the functional liquid, stir until uniform, filter the mixture using a 200 - 400 - mesh filter cloth, collect the filtrate to obtain the composition for firming and long - lasting anti - aging.

[0024] The Uncaria rhynchophylla extract contains dihydromyricetin, which has effects such as antioxidation and anti - inflammation, can reduce the damage of free radicals to the skin and delay aging; it can also promote adipocyte differentiation and lipid storage, enhance blood glucose uptake by activating the PPAR - γ pathway, thereby eliminating excess facial fat and enhancing the small - face effect. At the same time, it indirectly promotes collagen synthesis, enhancing skin elasticity and firmness.

[0025] Euglena gracilis polysaccharide has antioxidant and anti - inflammatory activities, can reduce skin inflammatory reactions and protect the skin from irritation; it can form a transparent film on the skin surface, instantly enhance the sense of firmness, smooth fine lines and dry lines, improve skin texture, and help the slow release of other ingredients, enabling the penetration of hydrolyzed collagen and providing a long - lasting moisturizing effect.

[0026] Hydrolyzed collagen is a low - molecular - weight collagen fragment, which can quickly form a film on the skin surface to provide an immediate firming effect; it can stimulate the skin's own collagen synthesis, enhance elasticity and firmness; it has moisturizing properties, helps the skin retain moisture, and promotes repair and regeneration.

[0027] Acetylhydroxyproline is the acetylation product of hydroxyproline, which can enhance the stability and three-dimensional sense of collagen, making it more elastic and tensile-resistant; by regulating the intracellular signaling pathway, it promotes collagen synthesis and plays a long-term anti-aging role; it has a high skin penetration rate and can effectively deliver active ingredients to the deep layer of the skin.

[0028] Sodium polyaspartate is a high-affinity anionic moisturizing polymer that can form a flexible film on the skin surface, lock in moisture through bioadhesion, keep the skin plump and moist, and reduce dry fine lines; it can also enhance the skin barrier function, reduce the damage of external stimuli to the skin, and protect the skin from environmental factors.

[0029] In the preparation method of the functional agent described in the present invention, the functions of each substance are as follows:

[0030] Chitosan is a natural polysaccharide with good biocompatibility and biodegradability. In the functional agent, chitosan serves as the main bioactive matrix, providing film-forming property and stability, and at the same time having antibacterial and moisturizing properties.

[0031] Fish collagen is a protein with high biocompatibility, which can enhance the mechanical properties and biological activity of the functional agent. It helps to improve the elasticity and firmness of the skin and promote skin repair at the same time.

[0032] Aqueous acetic acid solution is used to dissolve chitosan and fish collagen to form a uniform mixed solution. The acidic environment of acetic acid is helpful for the subsequent cross-linking reaction.

[0033] The cross-linking agent is used to carry out a cross-linking reaction with the amino groups in chitosan and fish collagen to form a stable network structure. This cross-linking effect enhances the film-forming property and stability of the functional agent, and at the same time promotes the uniform distribution and slow release of active ingredients.

[0034] The enhancer is used to further improve the mechanical properties and biocompatibility of the functional agent.

[0035] Polycaprolactone is a biodegradable polymer with good flexibility and biocompatibility. It is used to mix with the pretreatment product to further enhance the mechanical properties and stability of the functional agent.

[0036] 1,1,1,3,3,3-Hexafluoro-2-propanol, as a solvent, is used to dissolve polycaprolactone and other components to make them uniformly dispersed in the mixture. It can also improve the formability and uniformity of the fibers.

[0037] The mixed solvent is used for annealing treatment to further optimize the physical and chemical properties of the fibers. The addition of glycerol can enhance the flexibility and moisturizing property of the fibers, while absolute ethanol helps the rapid drying of the fibers.

[0038] These substances act synergistically in the preparation of the functional agent to form a nanofiber structure with high film-forming property, stability, and biocompatibility. Chitosan and fish collagen provide a bioactive matrix, the cross-linking agent and enhancer further optimize the structure and performance, while polycaprolactone and hexafluoroisopropanol ensure the uniformity and mechanical properties of the fibers. The mixed solvent and annealing treatment further optimize the physical and chemical properties of the fibers, making them more suitable for use in cosmetics with a firming and long-lasting anti-aging effect.

[0039] Compared with the prior art, it has the following beneficial effects:

[0040] 1) The present invention combines multiple active ingredients with anti-aging effects, such as Uncaria rhynchophylla extract, jellyfish peptide, and acetylhydroxyproline. These ingredients act synergistically to significantly improve the elasticity and firmness of the skin, reduce the appearance of wrinkles and fine lines, and avoid irritation to sensitive skin.

[0041] 2) The present invention combines multiple anti-aging active ingredients, such as Uncaria rhynchophylla extract, jellyfish peptide, and acetylhydroxyproline. These ingredients act synergistically to significantly improve the elasticity and firmness of the skin, reduce the appearance of wrinkles and fine lines. By optimizing the preparation process of the functional agent, the stability and sustained-release effect of these active ingredients are further enhanced, thus achieving a more lasting anti-aging effect.

[0042] 3) The present invention uses raw materials such as chitosan and fish collagen to prepare the functional agent. These raw materials have good biocompatibility and biodegradability, can form a protective film, enhance the skin's moisture retention and ability to resist external stimuli, and thus effectively improve the skin barrier function. This design not only enhances the anti-aging effect but also takes into account the repair and protection of the skin barrier, and is especially suitable for use on sensitive skin. Detailed implementation mode

[0043] Main sources of substances:

[0044] Gracilaria lemaneiformis polysaccharide, product number: TGYLZF343, Xi'an Tianguangyuan Biotechnology Co., Ltd.

[0045] Uncaria rhynchophylla extract, product number: wssw20220564, product grade: food grade, main use: food / health product / cosmetic raw material, Shaanxi Wensheng Biotechnology Co., Ltd.

[0046] Jellyfish peptide, single product number: HX-0617-481, Shanxi Hongxing Biotechnology Co., Ltd.

[0047] Sodium polyaspartate, mesh number: 100 mesh, product number: sfd84, Zhengzhou Weifeng Biotechnology Co., Ltd.

[0048] Chitosan, product number: 101, Guangzhou Bloomage Biotechnology Co., Ltd.

[0049] Fish collagen, product number: 180106, product grade: food grade, Nanjing Ximeinuo Biotechnology Co., Ltd.

[0050] Dialdehyde polyethylene glycol, model: xb-19, purity: 95%, Shaanxi Xingbei Aike Biotechnology Co., Ltd.

[0051] Polyglycolic acid, model: HXKJ34886, Wuhan Huaxiang Kejie Biotechnology Co., Ltd.

[0052] Polycaprolactone, grade: PD1-10, brand: BASF.

[0053] Dibenzaldehyde polyethylene glycol, product number: 80020141, Guangzhou Carbo Technology Co., Ltd.

[0054] Trehalose, product number: 23253178, Qingdao Haiweisen Biotechnology Co., Ltd.

[0055] Polyvinyl alcohol, product number: T5265, brand: Kuraray Japan.

[0056] Silymarin, single product number: zb-6595, Xi'an Zebang Biotechnology Co., Ltd.

[0057] Glucuronic acid, model: FET43, Wuhan Lanabai Pharmaceutical Chemical Co., Ltd.

[0058] The remaining raw materials in the examples and comparative examples of the present invention are all commercially available products.

[0059] The design idea of the present invention is to develop a composition with long-term firming and anti-aging effects and capable of improving skin barrier function by combining various anti-aging active ingredients and raw materials for enhancing skin barrier function. In terms of anti-aging, ingredients such as Uncaria rhynchophylla extract, jellyfish peptide, and acetylhydroxyproline are selected. These ingredients can act synergistically to significantly improve skin elasticity and firmness, and reduce the appearance of wrinkles and fine lines. At the same time, in order to enhance skin barrier function, functional agents are prepared using raw materials such as chitosan and fish collagen. These raw materials have good biocompatibility and biodegradability, can provide film-forming properties and stability, form a protective film, enhance skin moisturization and the ability to resist external stimuli, thereby improving skin barrier function. In addition, by optimizing the preparation process of the functional agent, such as crosslinking, electrospinning, and annealing treatment, the performance of the functional agent is further improved, enabling it to play a better role in anti-aging and enhancing skin barrier. Finally, the composition of the present invention not only achieves the goal of long-term firming and anti-aging, but also takes into account the repair and protection of the skin barrier, and is especially suitable for sensitive skin.

[0060] Example 1

[0061] The preparation method of a composition with firming and long-lasting anti-aging effects is as follows:

[0062] Step 1: Add 0.02 kg of disodium EDTA to 85.27 kg of water, heat to 90 °C, then add 0.15 kg of carbomer to the water, stir until the carbomer is completely dissolved to form a uniform solution. Add 0.06 kg of xanthan gum and 0.02 kg of sodium hyaluronate to the solution, stir until completely dissolved, add 2 kg of glycerol, continue to stir until uniform. When the temperature drops to 60 °C, add 0.09 kg of a complex, which is composed of Euglena gracilis polysaccharide and hydrolyzed collagen mixed in a mass ratio of 1:2, and stir until completely dispersed to obtain a mixed solution;

[0063] Step 2: Lower the temperature of the mixed solution prepared in Step 1 to 50 °C. Prepare a dilution by adding 0.15 kg of arginine to 10 kg of water, add the dilution to the mixed solution, stir until uniform, continue to cool to 45 °C, and sequentially add 0.3 kg of Uncaria rhynchophylla extract, 0.04 kg of jellyfish peptide, and 0.4 kg of acetylhydroxyproline, and stir until uniform to obtain a functional liquid;

[0064] Step 3: Lower the temperature of the functional liquid prepared in Step 2 to 40 °C. Add 0.6 kg of glyceryl stearate, 0.4 kg of sodium polyaspartate, 0.2 kg of silymarin, and 0.3 kg of water to the functional liquid, stir until uniform, filter the mixed solution using a 300-mesh filter cloth, and collect the filtrate to obtain the composition with firming and long-lasting anti-aging effects.

[0065] Example 2

[0066] The preparation method of a composition with firming and long-lasting anti-aging effects is as follows:

[0067] Step 1: Add 0.02 kg of disodium EDTA to 85.27 kg of water, heat to 90 °C, then add 0.15 kg of carbomer to the water, stir until the carbomer is completely dissolved to form a uniform solution. Add 0.06 kg of xanthan gum and 0.02 kg of sodium hyaluronate to the solution, stir until completely dissolved, add 2 kg of glycerol, continue to stir until uniform. When the temperature drops to 60 °C, add 0.09 kg of a complex, which is composed of Euglena gracilis polysaccharide and hydrolyzed collagen mixed in a mass ratio of 1:2, and stir until completely dispersed to obtain a mixed solution;

[0068] Step 2: Lower the temperature of the mixed solution prepared in Step 1 to 50 °C. Prepare a dilution by adding 0.15 kg of arginine to 10 kg of water, add the dilution to the mixed solution, stir until uniform, continue to cool to 45 °C, and sequentially add 0.3 kg of Uncaria rhynchophylla extract, 0.04 kg of jellyfish peptide, and 0.4 kg of acetylhydroxyproline, and stir until uniform to obtain a functional liquid;

[0069] Step 3: Lower the temperature of the functional liquid prepared in Step 2 to 40°C. Add 0.6 kg of glyceryl stearate, 0.4 kg of sodium polyaspartate, 0.2 kg of silymarin, and 0.3 kg of functional agent to the functional liquid, stir until uniform, filter the mixture using a 300-mesh filter cloth, collect the filtrate, and obtain a composition with firming and long-lasting anti-aging effects.

[0070] The preparation method of the functional agent is as follows:

[0071] S1: Mix 3 kg of chitosan, 3 kg of fish collagen, and 20 kg of 30 wt% acetic acid aqueous solution to form a mixed solution. Add 15 kg of 2 wt% dialdehyde polyethylene glycol aqueous solution to the mixed solution, stir evenly, and place the mixture at 40°C for 20 hours to form a pretreatment product.

[0072] S2: Mix the pretreatment product obtained in Step S1 with 0.7 kg of polyglycolic acid and 4 kg of polycaprolactone, stir at 130°C for 2 hours, add 1.2 kg of hexafluoroisopropanol to the above mixture, stir evenly and then perform electrospinning. Electrospinning parameters: voltage 20 kV, spinning speed 0.1 mL / min, receiving plate distance 12 cm. Let the electrospun fibers dry naturally, then soak them in a mixed solvent for annealing treatment. The mixed solvent is prepared by adding 1 kg of anhydrous ethanol and 5 kg of glycerol to 80 kg of water. After the annealing treatment is completed, dry the fibers and crush them to a particle size of 5 μm to finally obtain the functional agent.

[0073] Example 3

[0074] The preparation method of a composition with firming and long-lasting anti-aging effects is basically the same as that of Example 2, except that the preparation method of the functional agent is different.

[0075] The preparation method of the functional agent is as follows:

[0076] S1: Mix 3 kg of chitosan, 3 kg of fish collagen, and 20 kg of 30 wt% acetic acid aqueous solution to form a mixed solution. Add 15 kg of 2 wt% glyceraldehyde aqueous solution to the mixed solution, stir evenly, and place the mixture at 40°C for 20 hours to form a pretreatment product.

[0077] S2. Mix the pre-treated product obtained in step S1 with 0.7 kg of polyglycolic acid and 4 kg of polycaprolactone, stir at 130 °C for 2 hours, add 1.2 kg of hexafluoroisopropanol to the above mixture, stir evenly and then perform electrospinning. The electrospinning parameters are: voltage 20 kV, spinning speed 0.1 mL / min, receiving plate distance 12 cm. Let the electrospun fibers dry naturally, and then soak them in a mixed solvent for annealing treatment. The mixed solvent is prepared by adding 1 kg of anhydrous ethanol and 5 kg of glycerol to 80 kg of water. After the annealing treatment is completed, dry the fibers and crush them to a particle size of 5 μm to finally obtain the functional agent.

[0078] Example 4

[0079] The preparation method of a firming and long-lasting anti-aging composition is basically the same as that of Example 2, and the only difference is the preparation method of the functional agent.

[0080] The preparation method of the functional agent is as follows:

[0081] S1. Mix 3 kg of chitosan, 3 kg of fish collagen and 20 kg of 30 wt% acetic acid aqueous solution to form a mixed solution. Add 15 kg of 2 wt% dibenzaldehyde polyethylene glycol aqueous solution to the mixed solution, stir evenly, and place the mixture at 40 °C for 20 hours to form a pre-treated product.

[0082] S2. Mix the pre-treated product obtained in step S1 with 0.7 kg of polyglycolic acid and 4 kg of polycaprolactone, stir at 130 °C for 2 hours, add 1.2 kg of hexafluoroisopropanol to the above mixture, stir evenly and then perform electrospinning. The electrospinning parameters are: voltage 20 kV, spinning speed 0.1 mL / min, receiving plate distance 12 cm. Let the electrospun fibers dry naturally, and then soak them in a mixed solvent for annealing treatment. The mixed solvent is prepared by adding 1 kg of anhydrous ethanol and 5 kg of glycerol to 80 kg of water. After the annealing treatment is completed, dry the fibers and crush them to a particle size of 5 μm to finally obtain the functional agent.

[0083] Example 5

[0084] The preparation method of a firming and long-lasting anti-aging composition is basically the same as that of Example 2, and the only difference is the preparation method of the functional agent.

[0085] The preparation method of the functional agent is as follows:

[0086] S1. Mix 3 kg of chitosan, 3 kg of fish collagen and 20 kg of 30 wt% acetic acid aqueous solution to form a mixed solution. Add 15 kg of 2 wt% dialdehyde polyethylene glycol aqueous solution to the mixed solution, stir evenly, and place the mixture at 40 °C for 20 hours to form a pre-treated product.

[0087] S2. Mix the pre-treated product obtained in step S1 with 0.7 kg of trehalose and 4 kg of polycaprolactone, stir at 130 °C for 2 hours, add 1.2 kg of hexafluoroisopropanol to the above mixture, stir evenly and then perform electrospinning. The electrospinning parameters are: voltage 20 kV, spinning speed 0.1 mL / min, receiving plate distance 12 cm. Let the electrospun fibers air-dry naturally, then soak them in a mixed solvent for annealing treatment. The mixed solvent is prepared by adding 1 kg of anhydrous ethanol and 5 kg of glycerol to 80 kg of water. After the annealing treatment is completed, air-dry the fibers and crush them to a particle size of 5 μm to finally obtain the functional agent.

[0088] Example 6

[0089] The preparation method of a composition for firming and long-lasting anti-aging is basically the same as that of Example 2, and the only difference is the preparation method of the functional agent.

[0090] The preparation method of the functional agent is as follows:

[0091] S1. Mix 3 kg of chitosan, 3 kg of fish collagen and 20 kg of 30 wt% aqueous acetic acid solution to form a mixed solution. Add 15 kg of 2 wt% aqueous dialdehyde polyglycol solution to the mixed solution, stir evenly, and place the mixture at 40 °C for 20 hours to form a pre-treated product.

[0092] S2. Mix the pre-treated product obtained in step S1 with 0.7 kg of hyaluronic acid and 4 kg of polycaprolactone, stir at 130 °C for 2 hours, add 1.2 kg of hexafluoroisopropanol to the above mixture, stir evenly and then perform electrospinning. The electrospinning parameters are: voltage 20 kV, spinning speed 0.1 mL / min, receiving plate distance 12 cm. Let the electrospun fibers air-dry naturally, then soak them in a mixed solvent for annealing treatment. The mixed solvent is prepared by adding 1 kg of anhydrous ethanol and 5 kg of glycerol to 80 kg of water. After the annealing treatment is completed, air-dry the fibers and crush them to a particle size of 5 μm to finally obtain the functional agent.

[0093] Example 7

[0094] The preparation method of a composition for firming and long-lasting anti-aging is basically the same as that of Example 2, and the only difference is the preparation method of the functional agent.

[0095] The preparation method of the functional agent is as follows:

[0096] S1. Mix 3 kg of chitosan, 3 kg of fish collagen, and 20 kg of 30 wt% aqueous acetic acid solution to form a mixed solution. Add 15 kg of 2 wt% aqueous dialdehyde polyethylene glycol solution to the mixed solution, stir evenly, and place the mixture at 40 °C for 20 hours to form a pre-treatment product;

[0097] S2. Mix the pre-treatment product obtained in step S1 with 0.7 kg of polyvinyl alcohol and 4 kg of polycaprolactone, stir at 130 °C for 2 hours. Add 1.2 kg of hexafluoroisopropanol to the above mixture, stir evenly and then perform electrospinning. Electrospinning parameters: voltage 20 kV, spinning speed 0.1 mL / min, receiving plate distance 12 cm. Naturally air-dry the electrospun fibers, then soak them in a mixed solvent for annealing treatment. The mixed solvent is prepared by adding 1 kg of anhydrous ethanol and 5 kg of glycerol to 80 kg of water. After the annealing treatment is completed, air-dry the fibers and crush them to a particle size of 5 μm to finally obtain the functional agent.

[0098] Example 8

[0099] The preparation method of a composition with firming and long-lasting anti-aging effects is basically the same as that of Example 2, and the only difference lies in the preparation method of the functional agent.

[0100] The preparation method of the functional agent is as follows:

[0101] S1. Mix 3 kg of chitosan, 3 kg of fish collagen, and 20 kg of 30 wt% aqueous acetic acid solution to form a mixed solution. Add 15 kg of 2 wt% aqueous glucuronic acid solution to the mixed solution, stir evenly, and place the mixture at 40 °C for 20 hours to form a pre-treatment product;

[0102] S2. Mix the pre-treatment product obtained in step S1 with 0.7 kg of polyglycolic acid and 4 kg of polycaprolactone, stir at 130 °C for 2 hours. Add 1.2 kg of hexafluoroisopropanol to the above mixture, stir evenly and then perform electrospinning. Electrospinning parameters: voltage 20 kV, spinning speed 0.1 mL / min, receiving plate distance 12 cm. Naturally air-dry the electrospun fibers, then soak them in a mixed solvent for annealing treatment. The mixed solvent is prepared by adding 1 kg of anhydrous ethanol and 5 kg of glycerol to 80 kg of water. After the annealing treatment is completed, air-dry the fibers and crush them to a particle size of 5 μm to finally obtain the functional agent.

[0103] Test Example 1

[0104] Anti-aging efficacy detection

[0105] Evaluate the anti-aging effect of the compact and long-lasting anti-aging composition prepared by the present invention. Recruit 80 volunteers (aged 35 to 60 years old, female), randomly divide them into 8 groups with 10 people in each group, and use the compact and long-lasting anti-aging composition prepared by the invention respectively. The test period is 4 weeks.

[0106] During the test, the volunteers used it once in the morning and once in the evening every day for 4 consecutive weeks. During the test, no other cosmetics were used. After 4 weeks of use, the water content of the stratum corneum (Corneometer CM825) and skin elasticity (Cutometer dual MPA580) were tested on one randomly selected cheek.

[0107] No adverse reactions occurred in the volunteers during the test. The anti-aging test results are shown in Table 1.

[0108] Table 1

[0109] Experimental Scheme Cuticular Water Content (C.U.) Skin Elasticity R2 Example 1 54.46±3.67 0.50±0.02 Example 2 66.41±4.75 0.63±0.04 Example 3 63.32±4.26 0.58±0.03 Example 4 62.85±4.14 0.55±0.04 Example 5 63.92±4.20 0.56±0.04 Example 6 62.17±4.11 0.53±0.03 Example 7 62.05±4.06 0.52±0.03 Example 8 66.38±4.31 0.62±0.04

[0110] The larger the measured value of skin elasticity R2, the better the skin elasticity.

[0111] Test Example 2

[0112] Oil control test

[0113] Test instrument: Use a Sebumeter test probe to measure the sebum secretion amount.

[0114] Selection criteria for test subjects:

[0115] Age range: 20 - 38 years old.

[0116] Skin type: Evaluated as oily sensitive skin.

[0117] Sebum secretion amount: Forehead > 200 μg / cm 2 Cheeks > 150 μg / cm 2 .

[0118] Sample size: A total of 80 people, randomly divided into 8 groups with 10 people in each group.

[0119] Test steps:

[0120] Facial cleansing: The test subjects cleaned their faces with clean water.

[0121] Balancing stage: After cleansing, let it stand for 30 minutes to balance the skin condition.

[0122] Initial measurement: Measure the initial sebum secretion value of the forehead.

[0123] Sample application: Evenly apply the samples of the examples respectively, and the application amount is 60 mg / cm 2 .

[0124] Retention stage: Retain for 15 minutes after application.

[0125] Washing and measurement: Wash the forehead area and then measure the oil content 1 hour after washing.

[0126] Calculation formula:

[0127] Rate of change of oil = (oil content 1 hour after washing - initial oil secretion value) / initial oil secretion value × 100%

[0128] The test results are shown in Table 2.

[0129] Table 2

[0130] Experimental Scheme Rate of Change of Oil / % Example 1 -36.21 Example 2 -43.32 Example 3 -43.61 Example 4 -42.80 Example 5 -43.03 Example 6 -42.95 Example 7 -42.13 Example 8 -49.06

[0131] It can be seen from the data of Test Example 1 that the anti-aging effect of the composition for firming and long-lasting anti-aging prepared in Example 2 is the best.

[0132] In the present invention, the dialdehyde polyethylene glycol used in Example 2 exhibits a better anti-aging effect compared to the glyceraldehyde in Example 3 and the bisbenzaldehyde polyethylene glycol in Example 4. From the perspective of molecular structure analysis, the dialdehyde polyethylene glycol molecule contains two aldehyde groups (-CHO), and these aldehyde groups can undergo cross-linking reactions with the amino groups in chitosan and fish collagen to form a stable network structure. This cross-linking effect can not only enhance the film-forming property and stability of the composition, but also promote the uniform distribution and slow release of active ingredients, thereby more effectively exerting the anti-aging effect. In contrast, although glyceraldehyde also contains an aldehyde group, its molecule is smaller and its cross-linking ability is relatively weak, making it difficult to form a stable network structure. And although bisbenzaldehyde polyethylene glycol also contains an aldehyde group, the presence of the benzene ring in its molecular structure may affect its reaction activity with amino groups, thereby reducing the cross-linking efficiency. Therefore, due to its unique molecular structure and high cross-linking ability, dialdehyde polyethylene glycol shows a more significant effect in promoting skin firming and anti-aging.

[0133] In the present invention, the polyglycolic acid used in Example 2 exhibits better anti-aging efficacy compared to the trehalose in Example 5, the hyaluronic acid in Example 6, and the polyvinyl alcohol in Example 7. Analyzing from the perspective of molecular structure, polyglycolic acid is a polymer containing multiple hydroxyl groups. The hydroxyl groups in its molecular structure can form hydrogen bonds with the amino groups in chitosan and fish collagen, thereby enhancing the intermolecular interaction and forming a more stable network structure. This structure not only improves the film-forming property and stability of the composition but also promotes the uniform distribution and slow release of active ingredients, thus more effectively exerting the anti-aging efficacy. In contrast, although trehalose has moisture retention, its molecular structure is relatively simple and lacks the ability to form strong interactions with proteins; although hyaluronic acid has extremely strong moisture retention, its molecular weight is relatively large and it is difficult to penetrate deep into the skin to play a role; although polyvinyl alcohol has good film-forming property, it lacks strong interactions with biomolecules. Therefore, due to its unique molecular structure and efficient interaction ability, polyglycolic acid shows more significant effects in promoting skin firming and anti-aging.

[0134] In the present invention, the aqueous solution of glucuronic acid used in Example 8 combined with polyglycolic acid exhibits anti-aging efficacy similar to that of Example 2 and performs better in oil control. Analyzing from the perspective of molecular structure, the glucuronic acid molecule contains an aldehyde group and a carboxyl group, while polyglycolic acid is a polymer containing multiple hydroxyl groups. The aldehyde group in glucuronic acid can undergo a crosslinking reaction with the hydroxyl groups in polyglycolic acid to form a stable hydrogen bond or covalent bond network structure. This crosslinking not only enhances the film-forming property and stability of the composition but also promotes the uniform distribution and slow release of active ingredients, thus more effectively exerting the oil control efficacy. At the same time, the hydrophilicity of glucuronic acid helps to form a moisture retention film to regulate the water-oil balance of the skin and further promote the oil control effect. The combination of glucuronic acid and polyglycolic acid promotes the oil control effect through physical adsorption and moisture regulation mechanisms.

Claims

1. A firming and long-lasting anti-aging composition, characterized in that: Includes the following ingredients: chelating agent, thickener, moisturizer, sodium hyaluronate, complex, pH adjuster, Uncaria rhynchophylla extract, jellyfish peptide, acetylhydroxyproline, emulsifier, sodium polyaspartate, silymarin, functional agent, water; The preparation method of the functional agent is as follows: S1, mixing chitosan, fish collagen and acetic acid aqueous solution, adding crosslinking agent aqueous solution and stirring evenly to obtain a pretreated product; S2, mixing the pretreated material with the reinforcing agent and polycaprolactone, adding hexafluoroisopropanol and performing electrostatic spinning, air-drying the fiber and immersing it in a mixed solvent for annealing, and finally air-drying and crushing it to obtain a functional agent; The cross-linking agent is at least one of dialdehyde polyethylene glycol, glyceraldehyde, dibenzaldehyde polyethylene glycol, and glucuronic acid.

2. The firming and long-lasting anti-aging composition according to claim 1, characterized in that: The invention comprises the following ingredients in weight percentage: chelating agent 0.01% to 0.03%, thickener 0.14% to 0.28%, moisturizer 1% to 3%, sodium hyaluronate 0.01% to 0.03%, complex 0.06% to 0.12%, pH regulator 0.1% to 0.2%, Uncaria rhynchophylla extract 0.2% to 0.4%, jellyfish peptide 0.02% to 0.06%, acetylhydroxyproline 0.2% to 0.6%, emulsifier 0.4% to 0.8%, sodium polyaspartate 0.2% to 0.6%, silymarin 0.1% to 0.3%, functional agent 0.2% to 0.4%, and the balance is water; The preparation method of the functional agent is as follows, in parts by weight: S1, 2 to 4 parts of chitosan, 2 to 4 parts of fish collagen and 15 to 25 parts of 20 to 35 wt% acetic acid aqueous solution are mixed to form a mixed solution, 10 to 20 parts of 1 to 3 wt% cross-linking agent aqueous solution are added to the mixed solution, and the mixture is placed at 35 to 45 ° C for 10 to 30 hours to form a pretreated product; S2. The pretreated material obtained in step S1 is mixed with 0.5-1 parts of a reinforcing agent and 3-5 parts of polycaprolactone, and stirred at 120-140° C. for 1-6 hours. 1-1.5 parts of hexafluoroisopropanol are added to the mixture, and the mixture is stirred evenly before electrospinning. The electrospinning parameters are as follows: voltage 18-22 kV, spinning speed 0.05-0.2 mL / min, and receiving plate distance 10-15 cm. The spun fiber is naturally air-dried, and then immersed in a mixed solvent for annealing. The mixed solvent is prepared by adding 0.8-1.2 parts of anhydrous ethanol and 4-6 parts of glycerol to 70-90 parts of water. After the annealing is completed, the fiber is air-dried and crushed to a particle size of 3-8 μm to finally obtain a functional agent.

3. The firming and long-lasting anti-aging composition according to claim 1 or 2, characterized in that: The enhancer is at least one of polyglycolic acid, trehalose, hyaluronic acid and polyvinyl alcohol.

4. The firming and long-lasting anti-aging composition according to claim 1 or 2, characterized in that: The chelating agent includes at least one of disodium EDTA and tetrasodium EDTA.

5. The firming and long-lasting anti-aging composition according to claim 1 or 2, characterized in that: The thickener is at least one of carbomer, xanthan gum and cetearyl alcohol.

6. The firming and long-lasting anti-aging composition according to claim 1 or 2, characterized in that: The moisturizing agent is at least one of glycerol and propylene glycol; the pH adjusting agent is at least one of arginine and triethanolamine.

7. The firming and long-lasting anti-aging composition according to claim 1 or 2, characterized in that: The emulsifier is at least one of hydrogenated lecithin, sodium stearoyl glutamate, cetearyl glucose and glyceryl stearate.

8. The firming and long-lasting anti-aging composition according to claim 1 or 2, characterized in that: The complex is prepared by mixing fine algae polysaccharide and hydrolyzed collagen in a mass ratio of 1:1 to 3.

9. A method for preparing the firming and long-lasting anti-aging composition according to any one of claims 1 to 8, characterized in that: Here’s how: Step 1, add the chelating agent to water, heat to 80-95°C, then add part of the thickener to the water, stir until the thickener is completely dissolved to form a uniform solution, add the remaining thickener and sodium hyaluronate to the solution, stir until completely dissolved, add the moisturizer, continue to stir until uniform, add the complex when the temperature drops to 50-70°C, stir until completely dispersed, and obtain a mixed solution; Step 2, lowering the temperature of the mixed solution prepared in step 1 to 45-55° C., adding water to the pH adjuster to prepare a diluent, adding the diluent to the mixed solution, stirring until uniform, continuing to cool to 40-50° C., adding the Uncaria rhynchophylla extract, jellyfish peptide and acetylhydroxyproline in sequence, stirring until uniform, and obtaining a functional solution; Step 3: lower the temperature of the functional liquid prepared in step 2 to 30-45° C., add the emulsifier, sodium polyaspartate, silymarin and the functional agent to the functional liquid, stir until uniform, filter the mixed liquid using a 200-400 mesh filter cloth, collect the filtrate, and obtain a firming and long-lasting anti-aging composition.

10. Use of the firming, long-lasting and anti-aging composition according to any one of claims 1 to 8 in the field of cosmetics.