Targeting preparation based on colon enzyme and flora specific degradation

Abstract

The invention relates to the technical field of targeted preparations, and discloses a targeted preparation based on colonase and flora specific degradation, the preparation is composed of a sodium alginate-pectin composite shell material, and is prepared by using inulin, trehalose and whey protein as heat-resistant protective agents through a spray drying method. By optimizing the proportion of pectin and sodium alginate, the microcapsule can be kept stable in gastric juice, does not degrade and can be degraded at the colon part, so that the target of targeted delivery is achieved. Experiments show that the viable count of the preparation in the colon can reach 70% or above, the release rate can reach 60% or above, and the targeting delivery effect is good.

Description

Technical Field

[0001] The present invention relates to the technical field of targeted preparations, and more specifically, it relates to a targeted preparation based on colon enzyme and flora-specific degradation. Background Art

[0002] In recent years, probiotic products have attracted much attention due to their unique intestinal regulation function. However, the application of probiotic products in the prior art still faces two key technical bottlenecks:

[0003] Probiotics are intolerant to the gastrointestinal tract. After oral probiotics pass through the extremely acidic environment of gastric juice, their activity will decrease significantly, and most of them cannot reach the intestine to play a role.

[0004] The activity decreases during the production process. Probiotic foods are directly processed into liquid bacterium agents, freeze-dried powders, etc. through large-scale fermentation, and the activity of the strains will also decrease significantly. Summary of the Invention

[0005] To solve the above technical problems, the present invention provides a targeted preparation based on colon enzyme and flora-specific degradation.

[0006] The present invention provides a targeted preparation based on colon enzyme and flora-specific degradation. The preparation is composed of an alginate-pectin composite shell material, and uses inulin, trehalose, and whey protein as anti-thermal protectants, and is prepared by spray drying.

[0007] The beneficial effect of the present invention is that: by optimizing the ratio of pectin and alginate, the microcapsule can remain stable in gastric juice and does not degrade, while degrading in the colon part, thereby achieving the goal of targeted delivery.

[0008] By optimizing the ratio of the microcapsule wall material, the tolerance of probiotics in gastrointestinal fluid and the storage performance are significantly improved. On this basis, the preparation exhibits good targeted performance by virtue of the unique advantages based on colon enzyme and flora specificity.

[0009] At the same time, the spray drying process is deeply optimized from multiple dimensions, reducing the loss during the production and processing of probiotics while greatly improving the activity of probiotics.

[0010] The viable count of the preparation reaching the colon can reach more than 70% and the release rate can reach more than 60%, and the targeted delivery effect is good. Description of the Drawings

[0011] Figure 1 is the scanning electron micrograph of the microcapsule of the present invention;

[0012] Figure 2 is the tolerance line graph of the microcapsule of the present invention in simulated gastric juice;

[0013] Figure 3 It is a line graph showing the influence of the sodium alginate concentration of the present invention on the microcapsules;

[0014] Figure 4 It is a line graph showing the influence of the concentration of pectin of the present invention on the microcapsules;

[0015] Figure 5 It is a graph showing the influence of the heat-resistant protective agent of the present invention on the spray drying of microcapsules;

[0016] Figure 6 It is a graph showing the results of the orthogonal experiment of the heat-resistant protective agent of the present invention;

[0017] Figure 7 It is a graph showing the influence of the inlet air temperature on the microcapsules during the spray drying of the present invention;

[0018] Figure 8 It is a graph showing the influence of the gas supply volume on the microcapsules during the spray drying of the present invention;

[0019] Figure 9 It is a graph showing the influence of the feeding speed on the microcapsules during the spray drying of the present invention. Detailed implementation manners

[0020] Now, the subject matter described herein will be discussed with reference to exemplary embodiments. It should be understood that discussing these embodiments is only to enable those skilled in the art to better understand and thus implement the subject matter described herein. Without departing from the scope of protection of the content of this specification, changes can be made to the functions and arrangements of the elements discussed. Each example can omit, substitute, or add various processes or components as needed. Additionally, the features described in some examples can also be combined in other examples.

[0021] In at least one embodiment of the present invention, a targeted preparation based on colon enzyme and specific degradation of flora is disclosed. The preparation is composed of a sodium alginate-pectin composite shell material, and inulin, trehalose, and whey protein are used as heat-resistant protective agents, and is prepared by spray drying.

[0022] The present invention constructs a probiotic microencapsulation system based on a sodium alginate-pectin composite matrix. A synergistic strategy of heat-stable protective agents (inulin / trehalose / whey protein) is adopted.

[0023] First, through single-factor experiments (see Figure 3 and Figure 4 ), the optimal wall material ratio (sodium alginate:pectin = 20:1) is determined, and through single-factor and orthogonal experiments (see Figure 5 and Figure 6 ), the best ratio of the heat-resistant protective agent is determined. Secondly, microcapsules are prepared by spray drying technology (see Figure 7 , 8And 9, with an inlet air temperature of 120°C, an air supply volume of 75%, and a feeding rate of 7.5 ml / min). Furthermore, it was confirmed by scanning electron microscopy that the microcapsules were less than 10 μm. Finally, the effect of colon targeting was verified through in vitro simulated gastrointestinal fluid experiments (the release rate in the colon segment was > 60% after 12 h). This process increased the survival rate of probiotics during high-temperature processing to 80%.

[0024] Characterization and verification system

[0025] 1. Structural characterization

[0026] See Figure 4 , with the help of scanning electron microscopy, it can be clearly verified that the particle size of the preparation is less than 10 μm. This particle size characteristic makes the interaction force between particles weak during storage and use, thus effectively avoiding the occurrence of agglomeration phenomena. At the same time, the particle size being less than 10 μm also reflects from the side that the preparation has good consistency in particle size distribution and excellent homogeneity. This homogeneity is of great significance as it can ensure that the dose taken each time is accurate when consuming the preparation, thus fully exerting its efficacy.

[0027] 2. Performance testing

[0028] See Figure 5 , through in vitro simulated gastrointestinal fluid experiments, it can be seen that after the microcapsules made of the optimal wall material were used to encapsulate the strain, the survival rate of the strain in gastric juice exceeded 60%, showing a significant increase compared to ordinary bacterial solutions. Moreover, after being treated with simulated gastric juice for 1 hour, the release rate of the strain encapsulated by the optimal wall material microcapsules in simulated intestinal juice was higher than 60%, which fully indicates that this preparation has successfully achieved the effect of targeted delivery.

[0029] Experimental results

[0030] In this experiment, through the optimization of the wall material ratio, heat-resistant protective agent ratio, and microcapsule preparation process, the survival rate of probiotics during high-temperature processing was significantly increased, from the original relatively low level to 80%. And microcapsules with colon-targeting performance and effective protection of probiotics were successfully prepared, and all experimental indicators reached or exceeded the expected goals, providing a solid technical basis for further promoting the industrial production and application of probiotic preparations.

[0031] The above described the embodiments of the present invention, but these embodiments are not limited to the above specific implementation manners. The above specific implementation manners are merely illustrative and not restrictive. Under the inspiration of this embodiment, those of ordinary skill in the art can also make more equivalent embodiments in various forms, all of which fall within the protection scope of this embodiment.

Claims

1. A targeted preparation based on colon enzyme and flora-specific degradation, characterized in that: The preparation is composed of a sodium alginate-pectin composite shell material, and uses inulin, trehalose and whey protein as heat-resistant protective agents, and is prepared by a spray drying method.

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