Application of curcumin or salt thereof

Abstract

The invention relates to application of a composition containing curcumin or salt thereof in preparation of drugs, health care products or food additives for treating diseases caused by microplastic uptake of organisms. The method capable of reducing the damage of the microplastics to the cells is obtained, on one hand, the uptake of the cells to the microplastics is reduced, on the other hand, the cell damage caused by the microplastics is reduced, and the damage of the microplastics to organisms is greatly reduced.

Description

Technical Field

[0001] The present invention relates to biomedicine, health products, food additives or skin care products, and particularly relates to the application of curcumin or its salts. Background Art

[0002] Microplastics mainly include high molecular compounds such as polyethylene (PE), polypropylene (PP), polystyrene (PS), polyvinyl chloride (PVC), polyethylene terephthalate (PET), polyamide (PA), polyurethane (PU), etc., and are usually plastic particles with a diameter less than 5 mm. They are mainly formed due to human activities and the extensive use of plastic products, threatening the ecological environment and human health.

[0003] Microplastics with different particle sizes have different hazards to organisms. For example, nano-sized microplastics (<1μm) are more likely to penetrate biological barriers, enter cells or tissues, and cause deep toxicity effects due to their extremely small particle size. Research shows that after 100nm polystyrene microplastics are ingested by bees, they accumulate in the gut and significantly reduce the abundances of Lactobacillus and Bifidobacterium, disrupt the gut microbiota balance, lead to immune dysfunction, increase the risk of invasion of pathogenic bacteria (such as Hafnia alvei) into the lymph, and increase the mortality rate by 5 times. In mouse experiments, nano-sized microplastics disrupt the gut barrier function by inhibiting the differentiation of immune T cells and reducing secreted immunoglobulin A. Nano-sized microplastics (such as 0.25μm) can be taken up by a large number of colorectal cancer cells and passed on to daughter cells during cell division, enhancing the migration ability of cancer cells. In addition, nano-sized microplastics can penetrate the blood-brain barrier and accumulate in the brain tissues of Alzheimer's disease patients, accelerating the progression of neurodegenerative diseases. In addition, animal experiments show that nano-sized microplastics may reduce reproductive ability, resulting in decreased sperm motility in males and abnormal ovarian function in females. Nano-sized polyethylene and polyvinyl chloride particles can adsorb to arterial plaques, triggering local inflammatory reactions and increasing the risks of heart disease and stroke. Clinical data show that patients with arterial plaques containing microplastics have a 4.5-fold higher risk of postoperative cardiovascular events. For the skin, microplastics in the environment attach to the skin surface, which may cause skin inflammation, interfere with the endocrine system, or trigger allergic reactions, etc.

[0004] Therefore, microplastics seriously threaten the health of organisms, and there is still a need to seek effective methods to reduce the harm of microplastics to organisms. Summary of the Invention

[0005] In view of the technical problems existing in the prior art, the present invention provides an application of curcumin or its salts or a composition containing curcumin or its salts in the preparation of a drug, health product or food additive for preventing or reducing the uptake of microplastics by organism cells.

[0006] The application as described above, wherein the microplastics include one or more of polystyrene, polyethylene, polyvinyl chloride, polyester fiber, polypropylene, polyethylene terephthalate, polyamide, and polyurethane.

[0007] The application as described above, wherein the particle size of the microplastics is less than 1 micron; preferably, the particle size of the microplastics is 50 - 200 nanometers.

[0008] The application as described above, wherein the particle size of the microplastics is about 50 - 100 nanometers.

[0009] The application as described above, wherein the particle size of the microplastics is about 60 - 80 nanometers.

[0010] The application as described above, wherein the particle size of the microplastics is about 70 nanometers.

[0011] The present application further relates to the application of curcumin or its salt, or a composition containing curcumin or its salt, in the preparation of a drug, health product, or food additive for preventing and / or treating diseases in organisms caused by microplastic uptake.

[0012] The application as described above, wherein the diseases caused by microplastic uptake are diseases caused by the accumulation of microplastics in cells or diseases caused by the change of mitochondrial membrane potential in cells due to microplastic uptake.

[0013] The application as described above, wherein the diseases caused by microplastic uptake are selected from one or more of the following diseases: cardiovascular diseases, such as vascular calcification, arterial plaque; reproductive system diseases, such as oocyte maturation defects, decreased sperm motility; nervous system diseases, such as brain tissue inflammation, neuroinflammation, neurodegenerative diseases; bone metabolism-related diseases, such as arthritis, osteoporosis; digestive system diseases, such as intestinal flora imbalance; and cancer-related diseases, such as prostate cancer, colorectal cancer and its metastasis; skin-related diseases, such as skin inflammation, acne, allergy, etc.

[0014] The present application also relates to the application of curcumin or its salt, or a composition containing curcumin or its salt, in the preparation of skin care products, which can reduce the microplastic uptake of skin cells or alleviate the damage caused by microplastics to skin cells.

[0015] A composition for preventing and / or treating diseases in organisms caused by microplastic uptake, which comprises curcumin or its salt; and a pharmaceutically acceptable carrier.

[0016] A skin care product for reducing the microplastic uptake of skin cells or alleviating the damage caused by microplastics to skin cells, which comprises curcumin or its salt.

[0017] A method for preparing a drug, health product, food additive or skin care product that prevents or reduces the uptake of microplastics by biological cells, comprising: adding an effective amount of curcumin or a salt thereof to a composition for preparing a drug, health product, food additive or skin care product.

[0018] In this application, a method for reducing the damage of microplastics to cells is obtained. On the one hand, it reduces the uptake of microplastics by cells, and on the other hand, it alleviates the cell damage caused by microplastics, greatly reducing the harm of microplastics to organisms. Description of the Drawings

[0019] Next, the preferred embodiments of the present invention will be further described in detail with reference to the accompanying drawings, wherein:

[0020] Figure 1 is a bar chart showing the effect of curcumin on the uptake of microplastics by cells according to an embodiment of the present invention;

[0021] Figure 2 is a flow cytometer detecting the effect of curcumin and microplastics on mitochondrial membrane potential according to an embodiment of the present invention;

[0022] Figure 3 is a statistical chart of the ratio of red and green fluorescence showing the effect of curcumin and microplastics on mitochondrial membrane potential according to an embodiment of the present invention;

[0023] Figure 4 is a bar chart showing the effect of curcumin and microplastics on cell viability according to an embodiment of the present invention;

[0024] Figure 5 is an electron microscope photograph example of the degradation effect of curcumin on microplastics according to an embodiment of the present invention; and

[0025] Figure 6 is a statistical chart of the diameter of microplastics showing the degradation effect of curcumin on microplastics according to an embodiment of the present invention. Detailed Embodiments

[0026] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0027] In the following detailed description, reference may be made to the various specification drawings that form a part of the present application and illustrate specific embodiments of the present application. The specific embodiments of the present application are described in sufficient detail below to enable those of ordinary skill in the relevant art and technology to implement the technical solutions of the present application. It should be understood that other embodiments may also be utilized or changes may be made to the embodiments of the present application.

[0028] The professional terms involved herein have the following meanings:

[0029] As used herein, the English name of "curcumin" is curcumin, abbreviated as Cur herein, which is a natural phenolic antioxidant active compound mainly present in the rhizomes of turmeric, zedoary, mustard, curry, and turmeric. Natural curcumin contains three analogues: curcumin (Curcumin I), demethoxycurcumin (Curcumin II), and bisdemethoxycurcumin (Curcumin III), which differ in the number of methoxy groups (2, 1, 0). The present application does not limit the type of curcumin used. In some embodiments, the curcumin used in the present application is Curcumin I, purchased from MedChemExpress (MCE) with the product catalog number HY-N0005.

[0030] As a natural pigment, curcumin is widely used as a yellow colorant for foods such as curry powder and seasonings. At the same time, due to its anti-inflammatory and antioxidant functions, its anticancer potential, and its neuroprotective function, curcumin is also widely used in the fields of medicine and cosmetics. However, there is no relevant research on curcumin preventing cells from taking up microplastics.

[0031] As used herein, the salt of curcumin refers to a compound or mixture that can directly form curcumin under certain conditions, such as hydrolysis reactions, etc. This reaction can be generated in vivo by a certain stimulus or can also be caused by co-administering with other components.

[0032] As used herein, "cell" or "biological somatic cell" refers to a cell with biological activity, which is the basic unit of the structure and physiological function of an organism and the basis for growth and development. In some embodiments, the cell includes mitochondria. In some embodiments, the cell can take up microplastics in the environment and accumulate the microplastics therein. In some embodiments, after taking up a certain amount of microplastics, the membrane potential of the mitochondria in the cell will change.

[0033] The "monodisperse polystyrene microspheres" mentioned in this article are the microplastics used in the experiments of this application, with a particle size of about 70 nanometers. They include two types: non-fluorescent and fluorescent, both purchased from Tianjin Junyijia Technology Co., Ltd. Among them, the monodisperse polystyrene microspheres with green fluorescence are prepared by binding fluorescent molecules on the polystyrene microsphere matrix or surface. The product number or batch number of the non-fluorescent microspheres is 6-1-0006, and the product number or batch number of the fluorescent microspheres is 7-3-0006.

[0034] The "diseases" mentioned in this article refer to the lesions or health risks obtained by organisms caused by the biological activity or accumulation of microplastics in biological tissues or cells. Specifically, the lesions caused by microplastics have a process of "quantitative change leading to qualitative change". A small amount of microplastics in tissues or cells may not cause serious lesions, but they already pose a health threat to organisms. The diseases mentioned in this application include, but are not limited to, the following groups:

[0035] Cardiovascular diseases. After myocardial cells ingest microplastics, it leads to an increase in the accumulation of reactive oxygen species (ROS), the release of mitochondrial DNA into the cytoplasm, and the activation of the cGAS-STING signaling pathway, ultimately resulting in myocardial cell senescence. Animal experiments show that exposure to polystyrene microplastics activates macrophages in the aorta of ApoE knockout mice, accelerating the pathological progression of atherosclerotic plaques. Clinical studies also found that patients with polyethylene (PE) and polyvinyl chloride (PVC) in arterial plaques have a 4.5-fold increased risk of postoperative cardiovascular events, involving diseases such as vascular calcification and arterial plaques;

[0036] Reproductive system diseases. Long-term exposure at low doses can interfere with oocyte meiosis, resulting in defective oocyte maturation. Studies have found that polystyrene microplastics disrupt cell metabolism and division processes and affect fertilization ability by inhibiting histone deacetylase HDAC3, leading to high acetylation of H4K16. Exposure to polystyrene microplastics disrupts the spermatogenic environment in the testis, reducing sperm quantity and quality. Animal experiments show that the testicular structure of exposed mice is damaged and sperm motility significantly decreases;

[0037] Nervous system diseases. Exposure to styrene in the environment may cause neurotoxic effects such as headache, fatigue, and dizziness. Nanoplastics can penetrate the blood-brain barrier and accelerate the occurrence and development of neurodegenerative diseases. Studies have shown that polystyrene microplastics can promote the formation of β-amyloid peptide oligomers, which may be one of the mechanisms by which microplastics exacerbate Alzheimer's disease;

[0038] Bone metabolism-related diseases. Microplastics are transported to the bones through the blood, interfering with the metabolic balance of bone cells. It activates the NF-κB signaling pathway, accelerates the senescence of bone marrow mesenchymal stem cells, inhibits bone formation and promotes bone resorption, resulting in osteoporosis. Animal experiments show that the bone density of exposed mice decreases and the fracture risk increases;

[0039] Digestive system diseases: Polystyrene microplastics can damage the intestinal barrier function, reduce the abundance of probiotics (such as Lactobacillus and Bifidobacterium), and increase the proportion of pathogenic bacteria. This leads to a decrease in the secretion of intestinal secretory immunoglobulin A (sIgA), hinders T cell differentiation, weakens local and systemic immune responses, increases the risk of infection, such as causing intestinal flora dysbiosis;

[0040] Cancer-related diseases, such as prostate cancer, colorectal cancer and its metastases, etc.;

[0041] Skin-related diseases, such as skin inflammation, acne, skin allergic reactions, etc.

[0042] As used herein, "prevention" refers to administering a prophylactically effective amount of curcumin or its salt to an organism before exposure to microplastics or when the organism already contains a certain amount of microplastics but has not yet caused tissue lesions, so as to inhibit the uptake of microplastics by cells or tissues, or to mitigate the existing damage caused by microplastics to tissues or cells.

[0043] As used herein, "treatment" refers to administering a therapeutically effective amount of curcumin or its salt to an organism when microplastics have caused lesions in the skin, other tissues or cells, such as when the organism has acquired the diseases mentioned above.

[0044] The present invention provides an application of curcumin or its salt, or a composition containing curcumin or its salt, in the preparation of a drug, health product or food additive for preventing or reducing the uptake of microplastics by cells of an organism. The cells are cells at risk of being contaminated by microplastics, and can be cardiovascular cells, germ cells, skin cells, nervous system cells, digestive system-related cells, bone-related cells, etc. of an organism.

[0045] The present invention provides a composition containing curcumin or its salt, which can be used in the preparation of a drug, health product, food additive or skin care product for preventing diseases caused by the uptake of microplastics by an organism because it can reduce the uptake of microplastics by cells.

[0046] The present invention provides a composition containing curcumin or its salt, which can be used in the preparation of a drug, health product, food additive or skin care product for treating diseases caused by the uptake of microplastics by an organism because it can mitigate the damage of microplastics to cells.

[0047] In some embodiments, the microplastics include one or more of polystyrene, polyethylene, polyvinyl chloride, polyester fiber, polypropylene, polyethylene terephthalate, polyamide and polyurethane. In some embodiments, the microplastics include polystyrene.

[0048] In some embodiments, the particle size of the microplastics is less than 1 micron; preferably, the particle size of the microplastics is 50-200 nanometers; more preferably, the particle size of the microplastics is 50-100 nanometers; further preferably, the particle size of the microplastics is about 60-80 nanometers. Even more preferably, the particle size of the microplastics is about 70 nanometers.

[0049] In some embodiments, the diseases caused by microplastic uptake as described herein are diseases caused by the accumulation of microplastics in cells or by the change of mitochondrial membrane potential of cells caused by microplastic uptake.

[0050] In some embodiments, the diseases can be one or more of: cardiovascular diseases, such as arterial plaques, etc.; reproductive system diseases, such as defective oocyte maturation, decreased sperm motility, etc.; nervous system diseases, such as brain tissue inflammation, neuroinflammation, neurodegenerative diseases, etc.; bone metabolism-related diseases, such as arthritis, osteoporosis, etc.; digestive system diseases, such as intestinal flora imbalance, etc.; and cancer-related diseases, such as prostate cancer, colorectal cancer and its metastases, etc.; skin-related diseases, such as skin inflammation, acne, allergies, etc.

[0051] Therefore, skin care products containing a composition of curcumin or its salt can be prepared, and the skin care products can reduce the microplastic uptake of skin cells or alleviate the damage caused by microplastics to skin cells.

[0052] A composition containing curcumin or its salt can be prepared for use in preparing drugs, health products, food additives, etc. for preventing and / or treating diseases caused by microplastic uptake in organisms.

[0053] Furthermore, in some embodiments, the drugs, health products, and food additives containing the composition of curcumin or its salt further contain a suitable carrier, such as a medically or food-grade acceptable carrier.

[0054] A method for reducing microplastic uptake by isolated cells or the damage caused by microplastics to isolated cells includes: administering an effective amount of a composition containing curcumin or its salt to the isolated cells.

[0055] Example 1 Microplastic Uptake and Absorption Experiment

[0056] First, cells are obtained. In this example, the rat cardiomyocyte line H9C2 cells are seeded in a six-well cell culture plate and cultured overnight in a cell culture incubator at 37 degrees Celsius and 5% carbon dioxide, and then processed according to the groups.

[0057] In this example, a monodisperse polystyrene material microsphere (hereinafter referred to as microplastics, with a diameter of about 70 nm) labeled with green fluorescence was diluted using high-glucose DMEM medium (1% double antibiotics), and H9C2 cells were exposed to an environment with a final concentration of 0.05 mg / mL or 0.1 mg / mL microplastics. In the curcumin group, H9C2 cells were treated with both curcumin and microplastics, with the final concentration of curcumin being 10 μM. Specifically, according to different treatment methods, the cells were divided into 6 groups:

[0058] The first group was the control group, and the cells in the medium were not treated with anything.

[0059] The second group was the Cur group, and 10 μM curcumin was added to the medium and co-cultured with the cells.

[0060] The third group was the 0.05 mg / mL NPs group, which means 0.05 mg / mL of microplastics was added to the medium and co-cultured with the cells.

[0061] The fourth group was the 0.05 mg / mL NPs + Cur group, which means 0.05 mg / mL of microplastics and 10 μM curcumin were added to the medium and co-cultured with the cells.

[0062] The fifth group was the 0.1 mg / mL NPs group, which means 0.1 mg / mL of microplastics was added to the medium and co-cultured with the cells.

[0063] The sixth group was the 0.1 mg / mL NPs + Cur group, which means 0.1 mg / mL of microplastics and 10 μM curcumin were added to the medium and co-cultured with the cells.

[0064] After the above six groups of cells were treated for 1 hour, they were washed with PBS to wash away the unabsorbed fluorescent microplastics, and they were washed 3 times in total; the control group was treated synchronously. The cells were digested with trypsin, resuspended with 200 μL of PBS, and the fluorescence intensity of the FITC channel was detected using a flow cytometer. The average fluorescence intensity was used to represent the amount of fluorescent microplastics taken up by the cells.

[0065] Figure 1 It is a bar chart showing the effect of curcumin on the uptake of microplastics by cells according to an embodiment of the present invention. As Figure 1 shown, for the group with a fluorescent microplastic concentration of 0.05 mg / mL in the medium, 10 μM curcumin reduced the uptake of fluorescent microplastics by about 37.7%. For the group with a fluorescent microplastic concentration of 0.1 mg / mL in the medium, 10 μM curcumin reduced the uptake of fluorescent microplastics by about 47.1%, indicating that curcumin has a significant inhibitory effect on the uptake of microplastics by cells.

[0066] Example 2 Mitochondrial Membrane Potential Detection Experiment

[0067] Use monodisperse polystyrene microspheres without fluorescent labeling (hereinafter referred to as microplastics, with a diameter of about 70 nm), dilute them with DMEM medium, and expose H9C2 cells to an environment with a final concentration of 0.1 mg / mL microplastics. In the curcumin group, H9C2 cells were treated with both curcumin and microplastics, with the final concentration of curcumin being 10 μM. Specifically, according to different treatment methods, the cells were divided into 4 groups:

[0068] The first group was the control group, and the cells in the culture medium were not treated with anything;

[0069] The second group was the Cur group, and 10 μM curcumin was added to the culture medium and co-cultured with the cells;

[0070] The third group was the 0.1 mg / mL NPs group, which means 0.1 mg / mL of microplastics was added to the culture medium and co-cultured with the cells;

[0071] The fourth group was the 0.1 mg / mL NPs+Cur group, which means 0.1 mg / mL of microplastics and 10 μM of curcumin were added to the culture medium and co-cultured with the cells.

[0072] After the above four groups of cells were treated for 5 hours, the unabsorbed microplastics were washed away with PBS, and the washing was done 3 times in total. The cells were digested with trypsin without EDTA, stained with a mitochondrial membrane potential fluorescent probe (hereinafter referred to as JC-1), and detected using a flow cytometer.

[0073] JC-1 is a fluorescent probe used to detect changes in mitochondrial membrane potential. When the mitochondrial membrane potential is high, JC-1 forms polymers in the mitochondrial matrix to produce red fluorescence; while when the mitochondrial membrane potential is low, JC-1 exists as a monomer form to produce green fluorescence. The relative ratio of red and green fluorescence can be used to represent the proportion of mitochondrial depolarization. The decrease in mitochondrial membrane potential can be used as a detection index for the early stage of cell apoptosis.

[0074] Figure 2 It is a flow cytometry diagram of the effect of curcumin and microplastics on mitochondrial membrane potential according to an embodiment of the present invention; Figure 3 It is a statistical chart of the red / green fluorescence ratio of the effect of curcumin and microplastics on mitochondrial membrane potential according to an embodiment of the present invention. As Figure 2 and Figure 3 shown, treatment with 0.1 mg / mL microplastics can significantly decrease the mitochondrial membrane potential, while curcumin can alleviate this damage, reduce the proportion of mitochondrial depolarization, and play a protective role on the cells.

[0075] Example 3 CCK8 cell viability detection

[0076] Use monodisperse polystyrene microspheres without fluorescent labels (hereinafter referred to as microplastics, with a diameter of about 70 nm), dilute them with DMEM medium, and expose H9C2 cells to an environment with a final concentration of 0.1 mg / mL microplastics. For the curcumin group, H9C2 cells were treated with both curcumin and microplastics to make the final concentration of curcumin 10 μM. Specifically, according to different treatment methods, the cells were divided into 4 groups:

[0077] The first group was the control group (control), and the cells in the medium were not treated with anything;

[0078] The second group was the Cur group, and 10 μM curcumin was added to the medium and co-cultured with the cells;

[0079] The third group was the 0.1 mg / mL NPs group, which means adding 0.1 mg / mL microplastics to the medium and co-culturing with the cells;

[0080] The fourth group was the 0.1 mg / mL NPs + Cur group, which means adding 0.1 mg / mL microplastics and 10 μM curcumin to the medium and co-culturing with the cells.

[0081] After the above four groups of cells were treated for 14 hours, the cell viability was detected by the CCK8 method and measured using a microplate reader.

[0082] Figure 4 It is a bar chart showing the effects of microplastics and curcumin on cell viability according to an embodiment of the present invention, as Figure 4 shown, treatment with 0.1 mg / mL microplastics will decrease the cell viability, while adding curcumin can increase the cell viability and protect the cells.

[0083] Example 4 Degradation of Microplastics by Curcumin

[0084] Use monodisperse polystyrene microspheres without fluorescent labels (hereinafter referred to as microplastics, with a diameter of about 70 nm), dilute them with DMEM medium, with or without curcumin, to make the final concentration of H9C2 cells 0.1 mg / mL, and incubate at 37 °C for 1 hour or 5 hours.

[0085] The first group did not add curcumin, only contained DMEM medium and 0.1 mg / mL microplastics, and was incubated at 37 °C for 5 hours;

[0086] The second group contained 10 μM curcumin, DMEM medium and 0.1 mg / mL microplastics, and was incubated at 37 °C for 1 hour;

[0087] The third group contained 10 μM curcumin, DMEM medium and 0.1 mg / mL microplastics, and was incubated at 37 °C for 5 hours;

[0088] The above three groups of samples were observed by transmission electron microscopy to measure the diameter of microplastics. Figure 5 For the photographed examples, Figure 6 For the statistical charts, as Figure 5 and Figure 6 shown, co-incubation of 0.1 mg / mL microplastics and 10 μM curcumin in DMEM does not degrade the microplastics. That is to say, under such conditions, curcumin does not degrade the microplastics containing polystyrene.

[0089] The above embodiments are only for illustrating the present invention and are not intended to limit the present invention. Those of ordinary skill in the relevant technical field can make various changes and modifications without departing from the scope of the present invention. Therefore, all equivalent technical solutions should also fall within the scope of the disclosure of the present invention.

Claims

1. Use of curcumin or its salt or a composition comprising curcumin or its salt in the preparation of a medicine, health product or food additive for preventing or reducing the uptake of microplastics by biological cells; in, The microplastics include one or more of polystyrene, polyethylene, polyvinyl chloride, polyester fiber, polypropylene, polyethylene terephthalate, polyamide and polyurethane.

2. According to the use according to claim 1, the particle size of the microplastic is less than 1 micron.

3. According to the use according to claim 2, the particle size of the microplastic is 60-80 nanometers.

4. According to the use according to claim 2, the particle size of the microplastic is about 70 nanometers.

5. Use of curcumin or its salt or a composition comprising curcumin or its salt in the preparation of a medicine, health product or food additive for preventing diseases caused by microplastic ingestion in an organism; The diseases caused by microplastic ingestion are diseases caused by the accumulation of microplastics in cells or by changes in the mitochondrial membrane potential of cells due to microplastic ingestion.

6. The use according to claim 1, wherein the disease caused by microplastic ingestion is selected from one or more of the following diseases: cardiovascular disease, including plaque in the arteries; Reproductive system diseases, including oocyte maturation defects and decreased sperm motility; Neurological diseases, including brain tissue inflammation, neuroinflammation, neurodegenerative diseases, and arteriosclerosis; Bone metabolism-related diseases, including arthritis and osteoporosis; Digestive system diseases, including intestinal flora imbalance; Cancer-related diseases, including prostate cancer, colorectal cancer, and their metastases; and Skin-related diseases, including skin inflammation, acne, and allergies.

7. Use of a composition comprising curcumin or a salt thereof in the preparation of a skin care product, wherein the skin care product can reduce microplastic uptake by skin cells; in, The microplastics include one or more of polystyrene, polyethylene, polyvinyl chloride, polyester fiber, polypropylene, polyethylene terephthalate, polyamide and polyurethane.

8. A medicine, health product or food additive for treating diseases caused by microplastic ingestion in an organism, comprising curcumin or a salt thereof; and a medically acceptable carrier; in, The microplastics include one or more of polystyrene, polyethylene, polyvinyl chloride, polyester fiber, polypropylene, polyethylene terephthalate, polyamide and polyurethane.

9. A skin care product for reducing microplastic uptake by skin cells, comprising curcumin or a salt thereof; in, The microplastics include one or more of polystyrene, polyethylene, polyvinyl chloride, polyester fiber, polypropylene, polyethylene terephthalate, polyamide and polyurethane.

10. A method for preparing a medicine, health product, food additive or skin care product for preventing or reducing the uptake of microplastics by biological cells, comprising: An effective amount of curcumin or its salt is added to the composition for preparing medicines, health products, food additives or skin care products.

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