Polypeptide composition capable of reducing uric acid and preparation method thereof

Abstract

The invention discloses a polypeptide composition capable of reducing uric acid and a preparation method thereof. The polypeptide composition contains marine fish oligopeptide powder, ginseng small molecule active peptide, corn oligopeptide, sunflower peptide, nicotinamide, sodium bicarbonate, rheum officinale, semen plantaginis, rhizoma alismatis, moutan bark, rhizoma atractylodis, cortex phellodendri, polygonum cuspidatum, radix stephaniae tetrandrae, safflower carthamus, pawpaw, zaocys dhumnade, agkistrodon halys, rhizoma polygonati, longan aril, sophora flower bud, liquorice, cinnamon, semen coicis and purple eggplant. The technical scheme of the invention can obviously reduce uric acid, has the effects of clearing heat and eliminating dampness, promoting blood circulation to remove meridian obstruction and inhibiting uric acid synthesis, and can quickly reduce blood uric acid and regulate in-vivo purinan metabolism.

Description

Technical Field

[0001] The present invention relates to the technical field of biopharmaceuticals, and particularly relates to a polypeptide composition capable of reducing uric acid and a preparation method thereof. Background Art

[0002] In recent years, hyperuricemia has become a major hidden danger threatening human health. It can not only cause gout, but also may lead to kidney diseases. Hyperuricemia has become the "fourth high" after "the three highs", and its health threat cannot be ignored. High uric acid not only causes gout, but also interacts with "the three highs", causing serious damage to the body. If not intervened in time, it is likely to trigger a series of complications such as heart disease, cerebral infarction, diabetes, kidney disease, uremia, etc., threatening life. Summary of the Invention

[0003] The main object of the present invention is to propose a polypeptide composition capable of reducing uric acid and a preparation method thereof, aiming to solve the existing technical problems.

[0004] To achieve the above object, the polypeptide composition capable of reducing uric acid proposed by the present invention contains marine fish oligopeptide powder, ginseng small molecule active peptide, corn oligopeptide, sunflower peptide, nicotinamide, sodium bicarbonate, rhubarb, plantain seed, oriental waterplantain rhizome, moutan bark, atractylodes rhizome, phellodendron bark, polygonum cuspidatum, stephania root, safflower, papaya, zaocys dhumnades, agkistrodon halys, polygonatum sibiricum, longan aril, sophora japonica flower, liquorice, cinnamon, coix seed, and purple eggplant.

[0005] Optionally, the polypeptide composition contains: 2 - 8 parts of marine fish oligopeptide powder, 1 - 5 parts of ginseng small molecule active peptide, 2 - 6 parts of corn oligopeptide, 1 - 8 parts of sunflower peptide, 1 - 3 parts of nicotinamide, 1 - 4 parts of sodium bicarbonate, 1 - 4 parts of rhubarb, 1 - 4 parts of plantain seed, 3 - 6 parts of oriental waterplantain rhizome, 1 - 4 parts of moutan bark, 1 - 3 parts of atractylodes rhizome, 2 - 4 parts of phellodendron bark, 1 - 4 parts of polygonum cuspidatum, 1 - 6 parts of stephania root, 1 - 4 parts of safflower, 1 - 4 parts of papaya, 1 - 4 parts of zaocys dhumnades, 1 - 4 parts of agkistrodon halys, 1 - 4 parts of polygonatum sibiricum, 1 - 4 parts of longan aril, 1 - 4 parts of sophora japonica flower, 1 - 4 parts of liquorice, 1 - 4 parts of cinnamon, 1 - 4 parts of coix seed, and 1 - 4 parts of purple eggplant.

[0006] Optionally, the chemical structural formula of the corn oligopeptide is C33H47.

[0007] Optionally, the chemical structural formula of the ginseng small molecule active peptide is (C14H21N4S)n.

[0008] Optionally, the chemical structural formula of the sunflower peptide is HOOC-CH(R1)-NH-OC-CH(R2)-NH-OC-CH(R3)-NH2.

[0009] On the other hand, the present invention also proposes a preparation method of a polypeptide composition capable of reducing uric acid. The preparation method includes the following steps: S100: Prepare marine fish oligopeptide powder, ginseng small molecule active peptide, corn oligopeptide and sunflower peptide respectively; S200: Crush and mix evenly the marine fish oligopeptide powder, ginseng small molecule active peptide, corn oligopeptide and sunflower peptide prepared in step S100, and other raw materials according to the preset weight ratio; S300: Add water with a weight 3 - 5 times that of the total weight of the original components, and cook over a slow fire for 20 - 25 minutes to obtain a solution; S400: Add one or more solvents to the solution as a reaction medium to form polypeptide chains, and obtain a polypeptide composition through filtration and crystallization.

[0010] Optionally, the specific steps of the preparation method of the marine fish oligopeptide powder are as follows: Raw material preparation and water washing: Use marine low - fat fish meal as the raw material, add pure water according to the mass ratio of material to water, stir and wash, and then filter to obtain washed fish meal; Enzymatic hydrolysis treatment: Mix the washed fish meal with pure water, adjust the pH value, add protease for enzymatic hydrolysis to obtain an enzymatic hydrolysate; Enzyme inactivation and filtration: Heat up the enzymatic hydrolysate to inactivate the enzyme, then cool it, and filter after cooling to obtain a de - dregs enzymatic hydrolysate; Membrane separation and drying: Use an ultrafiltration membrane to perform membrane separation on the de - dregs enzymatic hydrolysate, and the permeate obtained is spray - dried to obtain marine fish oligopeptide powder.

[0011] Optionally, the specific steps of the preparation method of the ginseng small molecule active peptide are as follows: Take dried ginseng, crush it, soak it in petroleum ether and then filter. After drying the filter residue, mix it with an ethanol - aqueous solution, and perform microwave - ultrasonic treatment at a specific ultrasonic power, microwave power and temperature. Then filter to obtain a clear liquid. The residue is extracted with ethanol - water again, and the supernatant is combined. The supernatant is concentrated under reduced pressure to obtain a crude ginseng extract, and then filtered and concentrated to obtain a concentrated solution. Finally, the concentrated solution is spray - dried to obtain ginseng peptide powder.

[0012] Optionally, the specific steps of the preparation method of the corn oligopeptide are as follows: Extract corn protein: Stir the corn powder evenly with water, and then filter to remove the residue to obtain corn protein; Perform hydrolysis or fermentation treatment: Add the extracted corn protein to an enzyme or strain for hydrolysis or fermentation; Separate impurities: The fermented mixture needs to be efficiently separated through a spiral ultrafiltration membrane and a centrifugal separation device to remove impurities such as microorganisms and proteins; Perform a purification step to obtain high - purity corn oligopeptide.

[0013] Specifically, the specific steps of the preparation method of the sunflower peptide are as follows: Raw material pretreatment: Select fresh, pest-free, and moderately mature sunflower heads or sunflower seed meal as raw materials. After removing stems, fruits, or impurities, wash, dry in the sun, and crush them. Enzymatic hydrolysis treatment: Add the pretreated raw materials to deionized water. After adjusting to an appropriate pH value and temperature, add protease for enzymatic hydrolysis, and control the enzymatic hydrolysis time, dosage, and reaction conditions to obtain the required high-F-value oligopeptides or other functional peptides.

[0014] Separation and purification: After enzymatic hydrolysis, perform enzyme inactivation treatment and solid-liquid separation. Then, use filtration methods such as ceramic membranes, ultrafiltration membranes, and nanofiltration membranes to remove impurities, macromolecular substances, salts, etc., to obtain a relatively pure sunflower peptide solution. Concentrate the purified sunflower peptide solution, and then use methods such as spray drying for drying to obtain sunflower peptide powder.

[0015] Adopting the technical solution of the present invention has the following beneficial effects: The technical solution of the present invention The present invention is rich in: marine fish oligopeptide powder, ginseng small molecule active peptide (ginseng peptide), corn oligopeptide powder, sunflower pollen (sunflower peptide); marine fish oligopeptide powder can reduce uric acid, stimulate the synthesis of hepatocyte DNA, promote hepatocyte regeneration, and restore liver function. Ginseng small molecule active peptide (ginseng peptide) can directly penetrate into liver and kidney cells, reduce the degeneration of liver and kidney cells, and improve liver and kidney cell damage; regulate purine metabolism, reduce the production of uric acid, and promote the excretion of uric acid. Corn oligopeptide powder can quickly enter the blood circulation, relieve soreness, numbness, swelling, pain, and repair liver and kidney cells. Sunflower pollen (sunflower peptide) can inhibit purine synthesis, rapidly degrade uric acid in the blood, and dissolve uric acid stones. The present invention has a significant uric acid-lowering effect, has the functions of clearing heat and removing dampness, promoting blood circulation and dredging collaterals, and inhibiting uric acid synthesis, and can rapidly reduce blood uric acid and regulate purine metabolism in the body. Brief Description of the Drawings

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on the structures shown in these drawings.

[0017] Figure 1 It is a flowchart of the steps of a preparation method of a uric acid-lowering polypeptide composition according to an embodiment of the present invention. Detailed Embodiments

[0018] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present invention.

[0019] It should be noted that all directional indications (such as up, down, left, right, front, back...) in the embodiments of the present invention are only used to explain the relative positional relationship and movement conditions between components in a specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indications will also change accordingly.

[0020] In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the fact that those of ordinary skill in the art can implement them. When the combination of technical solutions results in contradictions or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0021] The present invention provides a polypeptide composition capable of reducing uric acid and a preparation method thereof.

[0022] In an embodiment of the present invention, the polypeptide composition capable of reducing uric acid contains marine fish oligopeptide powder, ginseng small molecule active peptide, corn oligopeptide, sunflower peptide, niacinamide, sodium bicarbonate, rhubarb, plantain seed, oriental waterplantain rhizome, moutan bark, atractylodes rhizome, phellodendron bark, polygonum cuspidatum, stephania root, safflower, papaya, zaocys dhumnades, gloydius blomhoffii, polygonatum sibiricum, longan aril, sophora japonica flower, licorice root, cinnamon, coix seed, and purple eggplant.

[0023] Specifically, the polypeptide composition contains: 2 - 8 parts of marine fish oligopeptide powder, 1 - 5 parts of ginseng small molecule active peptide, 2 - 6 parts of corn oligopeptide, 1 - 8 parts of sunflower peptide, 1 - 3 parts of niacinamide, 1 - 4 parts of sodium bicarbonate, 1 - 4 parts of rhubarb, 1 - 4 parts of plantain seed, 3 - 6 parts of oriental waterplantain rhizome, 1 - 4 parts of moutan bark, 1 - 3 parts of atractylodes rhizome, 2 - 4 parts of phellodendron bark, 1 - 4 parts of polygonum cuspidatum, 1 - 6 parts of stephania root, 1 - 4 parts of safflower, 1 - 4 parts of papaya, 1 - 4 parts of zaocys dhumnades, 1 - 4 parts of gloydius blomhoffii, 1 - 4 parts of polygonatum sibiricum, 1 - 4 parts of longan aril, 1 - 4 parts of sophora japonica flower, 1 - 4 parts of licorice root, 1 - 4 parts of cinnamon, 1 - 4 parts of coix seed, and 1 - 4 parts of purple eggplant.

[0024] Specifically, the chemical structural formula of the corn oligopeptide is C33H47.

[0025] Specifically, the chemical structural formula of the ginseng small molecule active peptide is (C14H21N4S)n.

[0026] Specifically, the chemical structural formula of the sunflower peptide is HOOC-CH(R1)-NH-OC-CH(R2)-NH-OC-CH(R3)-NH2.

[0027] On the other hand, as Figure 1 shown, the present invention also provides a method for preparing a polypeptide composition capable of reducing uric acid, and the preparation method includes the following steps: S100: Prepare marine fish oligopeptide powder, ginseng small molecule active peptide, corn oligopeptide and sunflower peptide respectively; S200: Crush and mix the marine fish oligopeptide powder, ginseng small molecule active peptide, corn oligopeptide and sunflower peptide prepared in step S100, and other raw materials according to a preset weight ratio; S300: Add 3-5 times the total weight of the original components of water, and cook with slow fire for 20-25 minutes to obtain a solution; S400: Add one or more solvents as reaction media to the solution to form polypeptide chains, and obtain the polypeptide composition through filtration and crystallization.

[0028] Specifically, the specific steps of the preparation method of the marine fish oligopeptide powder are as follows: Raw material preparation and water washing: Using marine low-fat fish meal as raw material, adding pure water according to the mass ratio of material to water, stirring and washing, and then filtering to obtain washed fish meal; Enzymatic hydrolysis treatment: Mix the washed fish meal with pure water, adjust the pH value, add protease for enzymatic hydrolysis to obtain an enzymatic hydrolysate; Enzyme inactivation and filtration: Heat the enzymatic hydrolysate to inactivate the enzyme, then cool it, and filter it after cooling to obtain a defatted enzymatic hydrolysate; Membrane separation and drying: Perform membrane separation on the defatted enzymatic hydrolysate with an ultrafiltration membrane, and the permeate obtained is spray-dried to obtain marine fish oligopeptide powder.

[0029] The marine fish oligopeptide powder prepared by this method has a high content of small molecule oligopeptides, good digestion and absorption effects, can improve immunity, and enhance the disease resistance ability. Specifically, the specific steps of the preparation method of the ginseng small molecule active peptide are as follows: Take dried ginseng, crush it, soak it with petroleum ether and then filter it. After drying the filter residue, mix it with an ethanol-aqueous solution, and perform microwave-ultrasonic treatment at a specific ultrasonic power, microwave power and temperature. Then filter to obtain a clear liquid, extract the residue with ethanol-water again and combine the supernatant. The supernatant is concentrated under reduced pressure to obtain a crude ginseng extract, and then filtered and concentrated to obtain a concentrated solution. Finally, the concentrated solution is spray-dried to obtain ginseng peptide powder.

[0030] Specifically, the specific steps of the preparation method of the corn oligopeptide are as follows: Extract corn protein. Mix corn powder with water evenly, and then filter to remove residues, thus obtaining corn protein. Conduct hydrolysis or fermentation treatment. Add the extracted corn protein to enzymes or strains for hydrolysis or fermentation. This step can catalyze the hydrolysis of proteins by adding specific enzymes, or conduct fermentation treatment by adding strains such as Bacillus subtilis and lactic acid bacteria. Usually, it is carried out for 12 - 24 hours under the conditions of 35°C - 40°C and pH 6.0 - 7.5. After hydrolysis or fermentation, it is necessary to filter to remove impurities and macromolecular substances to obtain preliminary corn oligopeptides.

[0031] Separate impurities. The fermented mixed solution needs to be efficiently separated through a spiral ultrafiltration membrane and a centrifugal separation device to remove impurities such as microorganisms and proteins. Conduct purification steps. By using high-tech methods such as ion exchange and gel chromatography, high-purity corn oligopeptides can be obtained.

[0032] Through the above steps, high-purity corn oligopeptides can be prepared.

[0033] Specifically, the specific steps of the preparation method of the sunflower peptide are as follows: Raw material pretreatment. Select fresh, pest-free, and moderately mature sunflower disks or sunflower seed meals as raw materials. After removing stems, fruits, or impurities, conduct washing, drying, crushing, etc. Enzymatic hydrolysis treatment. Add the pretreated raw materials to deionized water, adjust to an appropriate pH value and temperature, and then add protease (such as neutral protease, alkaline protease, or compound enzyme) for enzymatic hydrolysis, controlling the enzymatic hydrolysis time, dosage, and reaction conditions to obtain the required high-F-value oligopeptides or other functional peptides.

[0034] Separation and purification. After enzymatic hydrolysis, conduct enzyme inactivation treatment and solid-liquid separation. Then, use filtration methods such as ceramic membranes, ultrafiltration membranes, and nanofiltration membranes to remove impurities, macromolecular substances, and salts, etc., to obtain a relatively pure sunflower peptide solution. Concentrate the purified sunflower peptide solution, and then use methods such as spray drying for drying to obtain sunflower peptide powder.

[0035] Specifically, the working principle of the present invention is as follows: The principle of sodium bicarbonate in reducing uric acid lies in its ability to alkalize urine, increase the solubility of uric acid in urine, and thus promote the excretion of uric acid.

[0036] Specifically, uric acid is the end product of purine metabolism and usually needs to be excreted through the kidneys. When the production of uric acid is excessive or the excretion is reduced, it will lead to an increase in blood uric acid levels, which may cause hyperuricemia. Sodium bicarbonate is an alkaline drug. After oral administration, it can increase the pH value of urine and make the urine environment more alkaline. In an alkaline environment, the solubility of uric acid increases and it is not easy to crystallize and precipitate in urine, thus helping uric acid to be excreted out of the body with urine and indirectly playing a role in reducing blood uric acid.

[0037] Specifically, nicotinamide may inhibit the reabsorption of uric acid by renal tubules. The excretion of uric acid in the kidneys is through glomerular filtration and then partially reabsorbed. Nicotinamide may act as a competitive inhibitor, affecting uric acid transporters such as URAT1, thereby reducing reabsorption and increasing uric acid excretion.

[0038] In addition, nicotinamide affects the synthesis or catabolism of purines. For example, by regulating the activity of related enzymes, it reduces the production of purines, thus reducing the production of uric acid.

[0039] This invention is rich in: marine fish oligopeptide powder, ginseng small molecule active peptide (ginseng peptide), corn oligopeptide powder, sunflower pollen (sunflower peptide); Marine fish oligopeptide powder can reduce uric acid, stimulate the synthesis of hepatocyte DNA, promote hepatocyte regeneration, and restore liver function. Ginseng small molecule active peptide (ginseng peptide) can directly penetrate into liver and kidney cells, reduce the degeneration of liver and kidney cells, and improve the damage of liver and kidney cells; Regulate purine metabolism, reduce the production of uric acid, and promote the excretion of uric acid. Corn oligopeptide powder can quickly enter the blood circulation, relieve soreness, numbness, swelling, pain, and repair liver and kidney cells. Sunflower pollen (sunflower peptide) can inhibit purine synthesis, quickly degrade uric acid in the blood, and dissolve uric acid stones.

[0040] This invention has a significant effect of reducing uric acid, has the functions of clearing heat and removing dampness, promoting blood circulation and dredging collaterals, and inhibiting uric acid synthesis, and can quickly reduce blood uric acid and regulate purine metabolism in the body. It also has the following advantages: (1) It does not need to be digested and can be directly absorbed; (2) Fast absorption; (3) Complete absorption, almost 100%; (4) Absorption does not consume energy and can reduce the burden on gastrointestinal function; (5) It can be absorbed as a carrier.

[0041] The above are only the preferred embodiments of the present invention, and do not limit the patent scope of the present invention. Any equivalent structural transformation made under the inventive concept of the present invention, or direct / indirect application in other related technical fields, is included in the patent protection scope of the present invention.

Claims

1. A polypeptide composition capable of lowering uric acid, characterized in that: The polypeptide composition contains marine fish oligopeptide powder, ginseng small molecule active peptide, corn oligopeptide, sunflower peptide, nicotinamide, sodium bicarbonate, rhubarb, plantain seed, oriental water chestnut, peony bark, atractylodes, phellodendron, knotweed, stephania tetrandra, safflower, papaya, black-striped snake, agkistrodon, polygonatum, longan pulp, sophora japonica seed, licorice, cinnamon, coix seed and purple eggplant.

2. The uric acid-lowering polypeptide composition according to claim 1, characterized in that: By weight, the polypeptide composition contains: 2-8 parts of marine fish oligopeptide powder, 1-5 parts of ginseng small molecule active peptides, 2-6 parts of corn oligopeptides, 1-8 parts of sunflower peptides, 1-4 parts of sodium bicarbonate, 1-4 parts of rhubarb, 1-4 parts of plantain seeds, 3-6 parts of oriental rhizome, 1-4 parts of peony bark, 1-3 parts of atractylodes, 2-4 parts of phellodendron, 1-4 parts of knotweed, 1-6 parts of stephania tetrandra, 1-4 parts of safflower, 1-4 parts of papaya, 1-4 parts of black-striped snake, 1-4 parts of agkistrodon, 1-4 parts of polygonatum, 1-4 parts of longan pulp, 1-4 parts of sophora japonica seeds, 1-4 parts of licorice, 1-4 parts of cinnamon, 1-4 parts of coix seeds and 1-4 parts of purple eggplant.

3. The uric acid-lowering polypeptide composition according to claim 1, characterized in that: The chemical structural formula of the corn oligopeptide is C33H47.

4. The polypeptide composition capable of lowering uric acid according to claim 1, characterized in that: The chemical structural formula of the ginseng small molecule active peptide is (C14H21N4S)n.

5. The polypeptide composition capable of lowering uric acid according to claim 1, characterized in that: The chemical structural formula of the sunflower peptide is HOOC-CH(R1)-NH-OC-CH(R2)-NH-OC-CH(R3)-NH2‌.

6. The method for preparing the uric acid-lowering polypeptide composition according to any one of claims 1 to 5, characterized in that: The preparation method comprises the following steps: S100: preparing marine fish oligopeptide powder, ginseng small molecule active peptide, corn oligopeptide and sunflower peptide respectively; S200: The marine fish oligopeptide powder, ginseng small molecule active peptide, corn oligopeptide and sunflower peptide prepared in step S100, and other raw materials are crushed and mixed according to a preset weight ratio; S300: Add 3 to 5 times the total weight of the original components of water and simmer for 20 to 25 minutes to obtain a solution; S400: adding one or more solvents as reaction media to the solution to form polypeptide chains, and obtaining a polypeptide composition by filtering and crystallizing.

7. The method for preparing the uric acid-lowering polypeptide composition according to claim 6, characterized in that: The specific steps of the preparation method of the marine fish oligopeptide powder are: Raw material preparation and water washing: using marine low-fat fish meal as raw material, adding pure water according to the material-water mass ratio, stirring, washing and filtering to obtain washed fish meal; ‌ Enzymatic hydrolysis: Mix the washed fish meal with pure water, adjust the pH value, add protease for enzymatic hydrolysis, and obtain enzymatic hydrolyzate; Inactivation of enzyme and filtration: heating the enzymatic hydrolysate to inactivate the enzyme, then cooling it, filtering it after cooling, and obtaining the deslagging enzymatic hydrolysate; Membrane separation and drying: the deslagging enzymatic hydrolyzate is subjected to membrane separation with an ultrafiltration membrane, and the permeate obtained is spray-dried to obtain marine fish oligopeptide powder.

8. The method for preparing the uric acid-lowering polypeptide composition according to claim 6, characterized in that: The specific steps of the preparation method of the ginseng small molecule active peptide are: Ginseng is dried and crushed, and then filtered after being soaked in petroleum ether. The filter residue is dried and mixed with an ethanol-water solution, and subjected to microwave-ultrasonic treatment at a specific ultrasonic power, microwave power and temperature. The clear liquid is then filtered to obtain a residue, and the residue is extracted with ethanol-water and the supernatant is combined. The supernatant is concentrated under reduced pressure to obtain a crude ginseng extract, which is then filtered and concentrated to obtain a concentrated solution. Finally, the concentrated solution is spray-dried to obtain ginseng peptide powder.

9. The method for preparing the uric acid-lowering polypeptide composition according to claim 6, characterized in that: The specific steps of the preparation method of the corn oligopeptide are: Extracting corn protein, adding water to corn powder and stirring evenly, then filtering to remove residues, thereby obtaining corn protein; Performing hydrolysis or fermentation treatment, adding the extracted corn protein to enzymes or bacteria for hydrolysis or fermentation; To separate impurities, the fermented mixed liquid needs to be efficiently separated by a spiral ultrafiltration membrane and a centrifugal separation device to remove microorganisms and protein impurities; A purification step can be performed to obtain high-purity corn oligopeptides.

10. The method for preparing the uric acid-lowering polypeptide composition according to claim 6, characterized in that: The specific steps of the preparation method of the sunflower peptide are: Raw material pretreatment: select fresh, pest-free, and moderately mature sunflower discs or sunflower seed meal as raw materials, remove stems, fruits, or impurities, and then wash, dry, and crush them; Enzymatic hydrolysis: add deionized water to the pretreated raw materials, adjust to a suitable pH value and temperature, add protease for enzymatic hydrolysis, and control the enzymatic hydrolysis time, dosage and reaction conditions to obtain the desired high F value oligopeptide or other functional peptide; Separation and purification: after the enzymatic hydrolysis is completed, the enzyme is inactivated and the solid-liquid is separated, and then the impurities, macromolecular substances and salts are removed by filtration to obtain a relatively pure sunflower peptide solution; The purified sunflower peptide solution is concentrated and then dried by a spray drying method to obtain sunflower peptide powder.

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