Microfluidic detection device for food additives

Abstract

A disclosed microfluidic detection apparatus for food additives comprises a first microfluidic chip, the first microfluidic chip comprises a first lower chip body, the upper end of the first lower chip body is provided with a plurality of first liquid storage tanks, the first liquid storage tanks are connected with one end of a liquid outlet flow channel, and the liquid outlet flow channel is controllably communicated with a first mixing flow channel or a second mixing flow channel; the second micro-fluidic chip comprises a second upper chip body and a second lower chip body fixed on the lower side of the second upper chip body, and an antigen liquid storage tank, a first antibody liquid storage tank, a clean liquid storage tank, a second antibody liquid storage tank and a luminous substrate liquid storage tank which are arranged on the second lower chip body are controllably communicated with one end of a third mixing flow channel; a micro-channel is formed in the upper end of the second lower chip body at the other end of the third mixing flow channel; according to the invention, nitrite can be detected by two methods through one micro-fluidic chip, so that the reliability of nitrite detection is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of food additive detection, and in particular to a microfluidic detection device for food additives. Background Art

[0002] Chloramphenicol is a broad-spectrum antibiotic that inhibits both Gram-positive and Gram-negative bacteria. In food, chloramphenicol is an illegal additive and shall not be detected in animal-derived foods. Because chloramphenicol can inhibit the hematopoietic function of the human skeleton and cause diseases such as aplastic anemia, it is strictly prohibited from being used in food production and processing. Amantadine was the first antiviral drug used to inhibit influenza viruses, but amantadine is now listed as a prohibited veterinary drug and shall not be detected in animal-derived foods. Long-term intake of animal-derived foods with excessive amantadine may have adverse effects on human health. Sudan Red I: is an industrial dye mainly used to color oils, waxes, and plastics, and is not a food additive. It has potential carcinogenic effects and poses a serious threat to human health if added to food. Therefore, Sudan Red I is strictly prohibited from being used in food production and processing. In the prior art, the detection of the above food additives mainly relies on large-scale equipment, which not only takes a long time but also has complex operations, and generally can only detect a single substance, making it difficult to meet the requirements of rapid and efficient detection. Summary of the Invention

[0003] The purpose of this part is to outline some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Simplifications or omissions may be made in this part, as well as in the abstract and title of the present application, to avoid obscuring the purpose of this part, the abstract, and the title, but such simplifications or omissions shall not be used to limit the scope of the present invention.

[0004] In view of the above and / or existing problems in food additive detection, the present invention is proposed.

[0005] Therefore, the purpose of the present invention is to provide a microfluidic detection device for food additives, which realizes the detection of nitrite by two methods through the setting of the first microfluidic chip, improving the reliability of nitrite detection; through the setting of the second microfluidic chip, the solutions in different storage pools can be controllably discharged according to the set reaction sequence, indirectly measuring chloramphenicol, amantadine, and Sudan Red I.

[0006] To solve the above technical problems, the present invention provides the following technical solution: A microfluidic detection device for food additives, which includes, The first microfluidic chip includes a first upper chip body and a first lower chip body fixed to the lower side of the first upper chip body. At the upward end of the first lower chip body, there are arranged a number of first liquid storage pools, a number of liquid outlet channels corresponding to the first liquid storage pools one by one, a first mixing channel, a second mixing channel, a first waste liquid pool and a second waste liquid pool. One end of the liquid outlet channel is connected to the first liquid storage pool, and the other end of the liquid outlet channel is controllably connected to one end of the first mixing channel or one end of the second mixing channel. The other end of the second mixing channel is connected to the second waste liquid pool. The second microfluidic chip includes a second upper chip body and a second lower chip body fixed to the lower side of the second upper chip body. At the upward end of the second lower chip body, there are provided an antigen liquid storage pool, a first antibody liquid storage pool, a cleaning liquid storage pool, a second antibody liquid storage pool, a luminescent substrate liquid storage pool and a third mixing channel. The antigen liquid storage pool, the first antibody liquid storage pool, the cleaning liquid storage pool, the second antibody liquid storage pool and the luminescent substrate liquid storage pool are controllably connected to one end of the third mixing channel. At the upward end of the second lower chip body at the other end of the third mixing channel, there is a microchannel. Between the second upper chip body and the second lower chip body at the microchannel, there is a silica gel membrane coated with an antigen. At the upward end of the second lower chip body at the microchannel far from the third mixing channel, there is a third waste liquid pool.

[0007] As a preferred solution of the microfluidic detection device for food additives in the present invention, wherein: the first microfluidic chip further includes a first mobile valve, a second mobile valve and a third mobile valve. At the upward end of the first lower chip body between a number of liquid outlet channels and the first mixing channel or the second mixing channel, there is a first mobile channel, and the first mobile valve can just slide along the first mobile channel. On the first lower chip body between the first mixing channel and the first waste liquid pool, there is a second mobile channel, and the second mobile valve can just slide along the second mobile channel. On the first lower chip body between the first mixing channel and the first waste liquid pool and between the second mixing channel and the second waste liquid pool, there is a third mobile channel, and the third mobile valve can just slide along the third mobile channel. The first mobile valve controllably connects a number of liquid storage pools to the first mixing channel or the second mixing channel. The second mobile valve can connect the first mixing channel to the first waste liquid pool or connect the other end of the first mixing channel to the second mixing channel. The third mobile valve can connect the other end of the first mixing channel to the first waste liquid pool or connect the end of the second mixing channel to the second waste liquid pool.

[0008] As a preferred embodiment of the microfluidic detection device for food additives in the present invention, the following applies: The first mobile valve includes a first mobile transmission part outside the first lower chip body. A first mobile part that is just inserted into the first mobile channel is fixed on the first mobile transmission part. A number of liquid outlet holes corresponding to the first liquid storage pools are arranged on the first mobile part. There are three first liquid storage pools. When one end of two of the liquid outlet holes communicates with two of the first liquid storage pools, the other ends of the two liquid outlet holes communicate with the first mixing channel; when one end of the three liquid outlet holes communicates with the three first liquid storage pools one by one, the other ends of two of the liquid outlet holes communicate with the first mixing channel, and the other end of the other liquid outlet hole communicates with the head end of the second mixing channel.

[0009] As a preferred embodiment of the microfluidic detection device for food additives in the present invention, the following applies: The second mobile valve includes a second mobile transmission part outside the first lower chip body. A second mobile part that is just inserted into the second mobile channel is fixed on the second mobile transmission part. A first connecting mixed liquid hole and a second connecting mixed liquid hole are opened on the second mobile part. When one end of the first connecting mixed liquid hole communicates with the end of the first mixing channel, the other end of the first connecting mixed liquid hole communicates with the first waste liquid pool. The second connecting mixed liquid hole is offset from any mixing channel or waste liquid pool. When one end of the second connecting mixed liquid hole communicates with the end of the first mixing channel, the other end of the second connecting mixed liquid hole communicates with the second mixing channel.

[0010] As a preferred embodiment of the microfluidic detection device for food additives in the present invention, the following applies: The third mobile valve includes a third mobile transmission part outside the first lower chip body. A third mobile part that is just inserted into the third mobile channel is fixed on the third mobile transmission part. A first connecting waste liquid hole and a second connecting waste liquid hole are opened on the third mobile part. When both ends of the first connecting waste liquid hole communicate with the first mixing channel and the first waste liquid pool respectively, both ends of the second connecting waste liquid hole are offset from the second mixing channel and the second waste liquid pool respectively; when both ends of the second connecting waste liquid hole communicate with the second mixing channel and the second waste liquid pool respectively, both ends of the first connecting waste liquid hole are offset from the second mixing channel and the second waste liquid pool respectively.

[0011] As a preferred embodiment of the microfluidic detection device for food additives in the present invention, a fourth movable channel is provided on the second lower chip body between the liquid reservoir and the third mixing channel, the second microfluidic chip also includes a fourth movable valve, the fourth movable valve includes a fourth movable transmission part outside the second lower chip body, the fourth movable transmission part has a fourth plug hole, the fourth movable transmission part is fixed with a fourth movable part that is just inserted into the fourth movable channel, the fourth movable part has two first liquid holes and one second liquid hole, when one end of the two first liquid holes is respectively connected to the antigen liquid reservoir and the first antibody liquid reservoir, the other end of the first liquid hole is connected to one end of the third mixing channel, and the second liquid hole is staggered with any other liquid reservoir; when one end of the second liquid hole is connected to the cleaning liquid reservoir, the second antibody liquid reservoir or the luminescent substrate liquid reservoir, the other end of the second liquid hole is connected to one end of the third mixing channel, and the two first liquid holes are staggered with any other liquid reservoir.

[0012] As a preferred solution of the microfluidic detection device for food additives in the present invention, it also includes a detection shell, a fixed frame is fixedly connected to the detection shell, a movable seat that can move horizontally is connected to the fixed frame, and the movable seat is provided with a first injection component for injecting solution into the first microfluidic chip and detecting the food additive to be detected and a second injection component for injecting solution into each liquid reservoir in the second microfluidic chip and detecting the food additive to be detected, the first injection component includes a first connecting bracket connected to the movable seat, a plurality of first injection brackets are connected to the first connecting bracket, a plurality of first lifting motors are fixedly connected to the first injection bracket, a first injection connecting block is slidably connected to the first injection bracket below the first lifting motor, a first injection liquid tube is connected to the first injection connecting block, and the first injection A first injection screw corresponding to the first lifting motor is rotatably connected on the bracket, the first injection connecting block is threadedly connected to the corresponding first injection screw, and the first injection liquid tube can inject the solution into the corresponding first liquid storage tank; the second injection assembly includes a second connecting bracket connected to the movable seat, a plurality of second injection brackets are connected to the second connecting bracket, a plurality of second lifting motors are fixedly connected to the second injection bracket, a second injection connecting block is slidably connected to the second injection bracket under the second lifting motor, a second injection liquid tube is connected to the second injection connecting block, a second injection screw corresponding to the second lifting motor is rotatably connected to the second injection bracket, the second injection connecting block is threadedly connected to the corresponding second injection screw, and the second injection liquid tube can inject the solution into the corresponding second liquid storage tank.

[0013] As a preferred embodiment of the microfluidic detection device for food additives in the present invention, the following components are included: There are also two sets of clamping components that can move horizontally and are respectively used to clamp the first microfluidic chip and the second microfluidic chip. The clamping component includes a clamping bottom plate fixed inside the detection housing. On the upper side of the clamping bottom plate, there is a clamping support seat that can slide left and right. A clamping movement motor is fixedly connected to the clamping bottom plate. A clamping movement screw rod is rotatably connected to the clamping bottom plate, and the clamping movement screw rod is connected to the clamping movement motor. The clamping support seat is threadedly connected to the clamping movement screw rod. On the upper side of the clamping support seat, there is a clamping support plate fixedly connected. At the front and rear ends of the clamping support plate, there are respectively fixedly connected clamping support parts. On the outside of one clamping support part, there is a clamping motor connected. The clamping motor is connected to a clamping screw rod rotatably connected between the two clamping support parts. A clamping movement block is threadedly connected to the clamping screw rod. On the upper side of the end of the clamping movement block extending outside the clamping support plate, there is a connecting part fixedly connected. On the connecting part, there is an active clamping plate slidably connected along the upper side of the clamping support plate. On the upper side of the clamping support plate, there is a fixed clamping plate arranged opposite to the active clamping plate. On the fixed frame at one end of the first injection component in the left-right direction, there is a first detection camera fixedly connected. On the fixed frame at one end of the second injection component in the left-right direction, there is a second detection camera fixedly connected. The first microfluidic chip can be driven by the corresponding clamping seat to move below the first detection camera, and the second microfluidic chip can be driven by the corresponding clamping seat to move below the second detection camera.

[0014] As a preferred embodiment of the microfluidic detection device for food additives in the present invention, the following components are included: The first moving transmission part is provided with a first insertion hole, the second moving transmission part is provided with a second insertion hole, and the third moving transmission part is provided with a third insertion hole. The first injection component further includes a first upper connecting plate fixedly connected to one end of the first connecting bracket in the front-rear direction. A number of first guide rods are arranged on the first upper connecting plate. The lower side of the first guide rods is fixedly connected to a first lower connecting plate. The upper side of the first upper connecting plate is fixedly connected to a first transmission motor. The first transmission motor is connected to a first transmission screw rod rotatably connected between the first upper connecting plate and the first lower connecting plate. A first guide seat sliding along the first guide rods is threadedly connected to the first transmission screw rod. The first guide seat is rotatably connected to a first moving screw rod, a second moving screw rod, and a third moving screw rod. A first moving seat sliding on the first guide seat is threadedly connected to the first moving screw rod. The lower side of the first moving seat is fixedly connected to a first moving rod that can be inserted into the first insertion hole. A second moving seat sliding on the second guide seat is threadedly connected to the second moving screw rod. The lower side of the second moving seat is fixedly connected to a second moving rod that can be inserted into the second insertion hole. A third moving seat sliding on the third guide seat is threadedly connected to the third moving screw rod. The lower side of the third moving seat is fixedly connected to a third moving rod that can be inserted into the third insertion hole.

[0015] As a preferred embodiment of the microfluidic detection device for food additives in the present invention, the following is provided: a fourth insertion hole is formed in the fourth moving transmission part. The second injection assembly further includes a second upper connecting plate, which is fixedly connected to one end of the second connecting bracket in the front-rear direction. A plurality of second guide rods are arranged on the second upper connecting plate. A second lower connecting plate is fixedly connected to the lower side of the second guide rods. A second driving motor is fixedly connected to the upper side of the second upper connecting plate. A second driving lead screw, which is rotationally connected between the second upper connecting plate and the second lower connecting plate, is connected to the second driving motor. A second guide seat, which slides along the second guide rods, is threadedly connected to the second driving lead screw. A fourth moving lead screw, which is rotationally connected to the second guide seat, is rotationally connected to the second guide seat. A fourth moving seat, which is slidably connected to the second guide seat, is threadedly connected to the fourth moving lead screw. A fourth moving rod, which can be inserted into the fourth insertion hole, is fixedly connected to the lower side of the fourth moving seat.

[0016] Compared with the prior art, the present invention has the following technical effects: Through the setting of the first microfluidic chip, two methods are used to detect nitrite, improving the reliability of nitrite detection; Through the setting of the second microfluidic chip, the solutions in different liquid storage pools can be controllably discharged according to the set reaction sequence, indirectly measuring chloramphenicol, amantadine, and Sudan I; Through the setting of the first injection assembly and the second injection assembly, the corresponding solutions are respectively injected into the respective liquid storage pools of the first microfluidic chip and the second microfluidic chip. The insertion of the insertion rod into the corresponding moving valve realizes the movable connection between the movable insertion rod and the moving valve. By driving the corresponding moving valve to move through the insertion rod, the controllable inflow of the solution is realized. After the detection using the microfluidic chip is completed, only need to move the insertion rod upward to make it leave the moving valve, which is convenient for the disassembly of the microfluidic chip; It can be applied to the detection work of food additives, especially suitable for the detection of nitrite, chloramphenicol, amantadine, and Sudan I. Description of the Drawings

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings. Among them: Figure 1 It is a three-dimensional structure diagram of the first microfluidic chip in the present invention.

[0018] Figure 2 It is a three-dimensional structure diagram of the first microfluidic chip during the first-stage detection.

[0019] Figure 3 It is a three-dimensional structure diagram of the first microfluidic chip during the second-stage detection.

[0020] Figure 4 This is the three-dimensional structure diagram of the second microfluidic chip in the present invention.

[0021] Figure 5 This is the structure diagram of each mobile valve connected in the second microfluidic chip when one end of two first liquid passing holes is respectively communicated with the antigen storage pool and the first antibody storage pool.

[0022] Figure 6 This is the structure diagram of each mobile valve connected in the second microfluidic chip when one end of the second liquid passing hole is communicated with the cleaning storage pool.

[0023] Figure 7 This is the structure diagram of each mobile valve connected in the second microfluidic chip when one end of the second liquid passing hole is communicated with the second antibody storage pool.

[0024] Figure 8 This is the structure diagram of each mobile valve connected in the second microfluidic chip when one end of the second liquid passing hole is communicated with the luminescent substrate storage pool.

[0025] Figure 9 This is the three-dimensional structure of the present invention (wherein, the detection housing is set to be in a transparent state) Figure 1 .

[0026] Figure 10 This is the three-dimensional structure of the present invention (wherein, the detection housing is set to be in a transparent state) Figure 2 .

[0027] Figure 11 It is Figure 10 The partial enlarged view at position A in

[0028] Figure 12 This is the three-dimensional structure of the present invention after hiding the top cover and the detection housing Figure 1 .

[0029] Figure 13 It is Figure 12 The partial enlarged view at position B in

[0030] Figure 14 It is Figure 12 The partial enlarged view at position C in

[0031] Figure 15 This is the three-dimensional structure of the present invention after hiding the top cover and the detection housing Figure 2 .

[0032] Figure 16 It is Figure 15 The partial enlarged view at position D in

[0033] Figure 17Structural diagram for implementing the movement of the first moving valve, the second moving valve, and the third moving valve in the first injection assembly.

[0034] Figure 18 Structural diagram for implementing the fourth moving valve in the second injection assembly.

[0035] Figure 19 Stereo structure of two clamping assemblies arranged oppositely in the present invention Figure 1 。

[0036] Figure 20 Stereo structure of two clamping assemblies arranged oppositely in the present invention Figure 2 。

[0037] Figure 21 Stereo structure of the present invention after hiding the top cover and the detection housing Figure 2 。

[0038] Figure 22 For Figure 21 Partial enlarged view at position E in

[0039] Figure 23 Stereo structure of the present invention after hiding the top cover and the detection housing Figure 2 。

[0040] Figure 24 For Figure 23 Partial enlarged view at position F in

[0041] In the figure, 100 is the first microfluidic chip, 101 is the first lower chip body, 101a is the second waste liquid pool, 101b is the first waste liquid pool, 101c is the connection channel, 101d is the first liquid storage pool, 101e is the first moving channel, 101f is the second moving channel, 101g is the second mixing flow channel, 101h is the third moving channel, 101i is the first mixing flow channel, 102 is the first upper chip body, 102a is the second liquid extraction hole, 102b is the second upper push-pull part, 102c is the first liquid extraction hole, 103 is the third moving valve, 103a is the third moving transmission part, 103a-1 is the third insertion hole, 103b is the third moving part, 103b-1 is the first waste liquid connection hole, 103b-2 is the second waste liquid connection hole, 104 is the second moving valve, 104a is the second moving transmission part, 104a-1 is the second insertion hole, 104b is the second moving part, 104b-1 is the first mixed liquid connection hole, 105 is the first moving valve, 105a is the first moving transmission part, 105a-1 is the first insertion hole, 105b is the first moving part, 105b-1 is the liquid outlet hole, 200 is the second microfluidic chip, 201 is the fourth moving valve, 201a is the fourth moving part, 201a-1 is the first liquid passage hole, 201a-2 is the second liquid passage hole, 201b is the fourth moving transmission part, 201b-1 is the fourth insertion hole, 202 is the second lower chip body, 202a is the microchannel, 202b is the third mixing flow channel, 202c is the fourth moving channel, 202d is the luminescent substrate liquid storage pool, 202e is the second antibody liquid storage pool, 202f is the cleaning liquid storage pool, 202g is the first antibody liquid storage pool, 202h is the antigen liquid storage pool, 202i is the third waste liquid pool, 203 is the second upper chip body, 203a is the fourth upper push-pull part, 203b is the second waste liquid pool, 203c is the second sample injection hole, 203d is the third upper push-pull part, 300 is the support bottom plate, 400 is the detection housing, 500 is the top cover, 600 is the first injection assembly, 601 is the first connection bracket, 700 is the second injection assembly, 602 is the first injection bracket, 603 is the first lifting motor, 604 is the first injection lead screw, 605 is the first injection connection block, 606 is the first injection liquid pipe, 607 is the first syringe, 608 is the first moving motor, 609 is the first guide seat, 610 is the first lower connecting plate, 611 is the first guide rod, 612 is the first upper connecting plate, 613 is the first transmission lead screw, 614 is the second moving motor, 616 is the third moving rod, 616 is the second moving rod, 617 is the first moving rod, 618 is the first moving lead screw, 619 is the second moving lead screw, 620 is the third moving lead screw, 621 is the third moving motor, 622 is the second moving seat, 623 is the first moving seat, 624 is the third moving seat, 625 is the first peristaltic pump, 626 is the first liquid extraction lifting lead screw, 627 is the first liquid extraction pipe, 628 is the second liquid extraction pipe, 629 is the second liquid extraction connection block, 630 is the second liquid extraction lifting lead screw, 631 is the second liquid extraction vertical plate, 632 is the first liquid extraction motor, 633 is the first liquid extraction vertical plate, 634 is the first liquid extraction connection block, 635 is the second liquid extraction motor, 700 is the second injection assembly, 701 is the second connection bracket, 702 is the second syringe,703 Second lifting motor, 704 Second injection lead screw, 705 Second injection connecting block, 706 Second injection liquid pipe, 707 Second guide seat, 708 Fourth moving motor, 709 Second transmission motor, 710 Second upper connecting plate, 711 Second lower connecting plate, 712 Fourth moving rod, 713 Fourth moving lead screw, 714 Fourth moving seat, 715 Second guide rod, 716 Third peristaltic pump, 717 Third liquid extraction connecting block, 718 Third liquid extraction pipe, 719 Third liquid extraction lifting lead screw, 720 Third liquid extraction motor, 721 Third liquid extraction vertical plate, 722 Second liquid extraction bracket, 800 Fixed frame, 900 Clamping assembly, 901 Clamping motor, 902 Clamping lead screw, 903 Clamping moving block, 904 Connecting part, 905 Clamping support plate, 905a Fixed clamping plate, 905a-1 Second guide hole, 905a-2 First guide hole, 906 Clamping bottom plate, 907 Clamping support seat, 908 Clamping moving lead screw, 909 Movable clamping plate., Detailed implementation manners

[0042] To make the above objects, features, and advantages of the present invention more apparent and understandable, the following will describe the detailed implementation manners of the present invention with reference to the accompanying drawings of the specification.

[0043] In the following description, many specific details are set forth to facilitate a thorough understanding of the present invention. However, the present invention may be implemented in other ways different from those described herein. Those skilled in the art can make similar generalizations without departing from the connotation of the present invention. Therefore, the present invention is not limited by the specific embodiments disclosed below.

[0044] Secondly, the so-called "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation manner of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it an individual or selectively exclusive embodiment with other embodiments.

[0045] Embodiment 1 Referring to Figures 1 - 8 , this embodiment provides a microfluidic detection device for food additives, which can detect different food additives.

[0046] A microfluidic detection device for a food additive, comprising a first microfluidic chip 100, which includes a first mobile valve 105, a second mobile valve 104, a third mobile valve 103, a first upper chip body 102, and a first lower chip body 101 fixed to the lower side of the first upper chip body 102. On the left and right sides of the upper end of the first upper chip body 102, a first upper push-pull part and a second upper push-pull part 102b are respectively fixed, which are convenient for adjusting the position of the first microfluidic chip 100 in the left-right direction. At the upward end of the first lower chip body 101, there are arranged several first liquid storage pools 101d, several liquid outlet channels corresponding to the first liquid storage pools 101d one by one, a first mixing channel 101i, a second mixing channel 101g, a first waste liquid pool 101b, and a second waste liquid pool 203b101a. One end of the liquid outlet channel is connected to the first liquid storage pool 101d, and the other end of the liquid outlet channel is controllably connected to one end of the first mixing channel 101i or one end of the second mixing channel 101g. The other end of the second mixing channel 101g is connected to the second waste liquid pool 203b101a. On the upward end of the first lower chip body 101 between several liquid outlet channels and the first mixing channel 101i or the second mixing channel 101g, a first mobile channel 101e is opened. The first mobile valve 105 can just slide along the first mobile channel 101e. On the first lower chip body 101 between the first mixing channel 101i and the first waste liquid pool 101b, a connection channel 101c is provided. On the upper end of the first lower chip body 101 between the head end of the connection channel 101c and the end of the first mixing channel 101i, a second mobile channel 101f is opened. The second mobile valve 104 can just slide along the second mobile channel 101f. On the first lower chip body 101 between the first mixing channel 101i and the first waste liquid pool 101b and between the second mixing channel 101g and the second waste liquid pool 203b101a, a third mobile channel 101h is opened. The third mobile valve 103 can just slide along the third mobile channel 101h. The first mobile valve 105 makes several liquid storage pools controllably connected to the first mixing channel 101i or the second mixing channel 101g. The second mobile valve 104 can make the first mixing channel 101i connected to the first waste liquid pool 101b or the other end of the first mixing channel 101i connected to the second mixing channel 101g. The third mobile valve 103 can make the other end of the first mixing channel 101i connected to the first waste liquid pool 101b or the end of the second mixing channel 101g connected to the second waste liquid pool 203b101a; The second microfluidic chip 200 includes a fourth movable valve 201, a second upper chip body 203 and a second lower chip body 202 fixed to the lower side of the second upper chip body 203, and the left and right sides of the upper end of the second upper chip body 203 are respectively fixed with a third upper push-pull portion 203d and a fourth upper push-pull portion 203a for conveniently adjusting the position of the second microfluidic chip 200 in the left and right directions, and the upper end of the second lower chip body 202 is provided with an antigen reservoir 202h, a first antibody reservoir 202g, a cleaning reservoir 202f, a second antibody reservoir 202e, a luminescent substrate reservoir 202d and a third mixing channel 202b, the antigen reservoir 202h, the first antibody reservoir 202g, the cleaning reservoir 202f, the second antibody reservoir 202e, and the luminescent substrate reservoir 202d are controllably connected to one end of the third mixing channel 202b, and a microchannel 202a is provided at an upward end of the second lower chip body 202 at the other end of the third mixing channel 202b, and a silica gel membrane coated with antigens (three vertical lines are respectively used to detect the antigens of the three hazards) is sandwiched between the second upper chip body 203 and the second lower chip body 202 at the microchannel 202a. The microchannel 202a is away from the second lower chip body 203 of the third mixing channel 202b. A third waste liquid pool 202i is provided at an upward end of the chip body 202, a fourth moving channel 202c is provided on the second lower chip body 202 between the liquid storage pool and the third mixing channel 202b, the fourth moving valve 201 includes a fourth moving transmission part 201b outside the second lower chip body 202, the fourth moving transmission part 201b is provided with a fourth plug hole 201b-1, a fourth moving part 201a which is just inserted into the fourth moving channel 202c is fixed on the fourth moving transmission part 201b, and the fourth moving part 201a is provided with two first liquid holes 201a-1 and one second liquid hole 201a-2. When one end of the two first liquid holes 201a-1 are connected to the antigen reservoir 202h and the first antibody reservoir 202g respectively, the other end of the first liquid hole 201a-1 is connected to one end of the third mixing channel 202b, and the second liquid hole 201a-2 is staggered with any other reservoir; when one end of the second liquid hole 201a-2 is connected to the cleaning reservoir 202f or the second antibody reservoir 202e or the luminescent substrate reservoir 202d, the other end of the second liquid hole 201a-2 is connected to one end of the third mixing channel 202b, and the two first liquid holes 201a-1 are staggered with any other reservoir.

[0047] Use the second microfluidic chip 200 to detect chloramphenicol, amantadine, and Sudan I. The specific detection process is as follows: Inject chloramphenicol, amantadine, and Sudan I into the antigen reservoir 202h, inject monoclonal antibodies against chloramphenicol, amantadine, and Sudan I into the first antibody reservoir 202g, inject PBST solution into the cleaning reservoir 202f, inject HRP-labeled goat anti-mouse IgG into the second antibody reservoir 202e, and inject the chemiluminescent substrate luminol into the luminophore reservoir 202d. In the initial state, the fourth movable valve 201 isolates each reservoir from the third mixing channel 202b; during detection, a peristaltic pump is connected to the third waste liquid reservoir 202i respectively. Control the movement of the fourth movable valve 201 to connect the antigen reservoir 202h and the first antibody reservoir 202g to the third mixing channel 202b simultaneously. Stop the movement of the fourth movable valve 201, control the peristaltic pump to operate, so that the analyte and the excessive antibody enter the third mixing channel 202b for mixing, and finally stay in the wavy microchannel 202a on the reaction layer for incubation (incubate at 37 °C for 15 minutes). After incubation, control the movement of the fourth movable valve 201 to connect the cleaning reservoir 202f to the third mixing channel 202b. Stop the movement of the fourth movable valve 201, and pump out the PBST in the cleaning reservoir 202f to clean the reaction layer. After cleaning, stop the operation of the peristaltic pump. Control the movement of the fourth movable valve 201 to connect the second antibody reservoir 202e to the third mixing channel 202b. Stop the movement of the fourth movable valve 201, and the peristaltic pump operates. The HRP-labeled goat anti-mouse IgG is pumped out to the microchannel 202a to react with the antigen. Control the peristaltic pump to make the antibody stay still in the microchannel 202a for incubation (incubate at room temperature for 10 minutes). After the second incubation, stop the operation of the peristaltic pump. Control the movement of the fourth movable valve 201 to connect the cleaning reservoir 202f to the third mixing channel 202b. Stop the movement of the fourth movable valve 201, and pump out the PBST in the cleaning reservoir 202f to clean the reaction layer. Stop the operation of the peristaltic pump, and after cleaning, control the movement of the fourth movable valve 201 to connect the luminophore reservoir 202d to the third mixing channel 202b. The peristaltic pump operates, and the chemiluminescent substrate is pumped out to generate chemiluminescent signals (dot matrix) (nine light points) on the reaction layer (microchannel 202a) to achieve the detection of the three hazardous substances.

[0048] In this application, different competitive antigens are arranged and fixed on the silica gel membrane sandwiched in the second microfluidic chip 200, and combined with the spatially resolved acquisition of chemiluminescent signals, the synchronous detection of three hazardous substances is realized, and three groups of independent quantitative data can be output in a single detection.

[0049] Specifically, the first moving valve 105 includes a first moving transmission part 105a outside the first lower chip body 101. A first moving part 105b that is just inserted into the first moving channel 101e is fixed on the first moving transmission part 105a. A number of liquid outlet holes 105b-1 corresponding to the first liquid storage pools 101d one by one are arranged on the first moving part 105b. There are three first liquid storage pools 101d. When one end of two of the liquid outlet holes 105b-1 communicates with two of the first liquid storage pools 101d, the other ends of the two liquid outlet holes 105b-1 communicate with the first mixing channel 101i. When one end of the three liquid outlet holes 105b-1 communicates with the three first liquid storage pools 101d one by one, the other ends of two of the liquid outlet holes 105b-1 communicate with the first mixing channel 101i, and the other end of the other liquid outlet hole 105b-1 communicates with the head end of the second mixing channel 101g. The second moving valve 104 includes a second moving transmission part 104a outside the first lower chip body 101. A second moving part 104b that is just inserted into the second moving channel 101f is fixed on the second moving transmission part 104a. A first connecting mixed liquid hole 104b-1 and a second connecting mixed liquid hole are formed on the second moving part 104b. When one end of the first connecting mixed liquid hole 104b-1 communicates with the end of the first mixing channel 101i, the other end of the first connecting mixed liquid hole 104b-1 communicates with the first waste liquid pool 101b. The second connecting mixed liquid hole is staggered from any mixing channel or waste liquid pool. When one end of the second connecting mixed liquid hole communicates with the end of the first mixing channel 101i, the other end of the second connecting mixed liquid hole communicates with the second mixing channel 101g. The third moving valve 103 includes a third moving transmission part 103a outside the first lower chip body 101. A third moving part 103b that is just inserted into the third moving channel 101h is fixed on the third moving transmission part 103a. A first connecting waste liquid hole 103b-1 and a second connecting waste liquid hole 103b-2 are formed on the third moving part 103b. When both ends of the first connecting waste liquid hole 103b-1 communicate with the first mixing channel 101i and the first waste liquid pool 101b respectively, both ends of the second connecting waste liquid hole 103b-2 are staggered from the second mixing channel 101g and the second waste liquid pool 203b101a. When both ends of the second connecting waste liquid hole 103b-2 communicate with the second mixing channel 101g and the second waste liquid pool 203b101a respectively, both ends of the first connecting waste liquid hole 103b-1 are staggered from the second mixing channel 101g and the second waste liquid pool 203b101a.

[0050] Refer to Figure 2 and Figure 3, mixed solutions of nitrite and hydrochloric acid, potassium ferrocyanide solution (colorless), and a mixed solution of uric acid, luminol, and sodium hydroxide are respectively injected into the three first liquid storage pools 101d. The detection process in the first stage is as follows: Control the movement of the first moving valve 105, the second moving valve 104, and the third moving valve 103. By moving the first moving valve 105, the two left liquid storage pools are connected to the first mixing channel 101i. The first moving valve 105 stops moving, and the mixed solution of nitrite and hydrochloric acid and the potassium ferrocyanide solution enter the first mixing channel 101i for mixing reaction. The nitrite oxidizes potassium ferrocyanide to potassium ferricyanide, and the solution changes from colorless before mixing to yellow. By moving the second moving valve 104 and the third moving valve 103, the first mixing channel 101i is connected to the head end of the connection channel 101c, and the tail end of the connection channel 101c is connected to the first waste liquid pool 101b. The second moving valve 104 and the third moving valve 103 stop moving, and the reacted solution enters the first waste liquid pool 101b. The preliminary detection of nitrite is realized through the change in yellowness, and the presence or absence of nitrite is initially judged. Start the detection in the second stage. The detection process in the second stage is as follows: Move the first moving valve 105, the second moving valve 104, and the third moving valve 103. When the two left first liquid storage pools 101d are connected to the first mixing channel 101i and the right first liquid outlet pool is connected to the second mixing channel 101g, and the second moving valve 104 connects the tail end of the first mixing channel 101i to the second mixing channel 101g and the tail end of the second mixing channel 101g to the second waste liquid pool 203b101a, the first moving valve 105 stops moving, the second moving valve 104 stops moving, and the third moving valve 103 stops moving. The potassium ferricyanide generated after the reaction in the two left first liquid storage pools 101d is mixed with the solution in the rightmost first liquid storage pool 101d. Potassium ferricyanide oxidizes luminol under the enhancement of uric acid to produce chemiluminescence, thereby indirectly determining nitrite. The two methods for determining nitrite are realized through the first microfluidic chip 100, improving the reliability of detecting nitrite.

[0051] When detecting using the first microfluidic chip 100, through the following two methods: First, the colorimetric method: Based on the oxidation-reduction reaction between nitrite and potassium ferrocyanide in an acidic medium (generating yellow potassium ferricyanide), quantitative analysis is carried out through the change in solution color (absorbance). The advantage lies in intuitiveness and rapid qualitative judgment.

[0052] Second, the chemiluminescence method: Utilize the chemiluminescence generated by potassium ferricyanide and luminol under the enhancement of uric acid, and quantify through the intensity of the optical signal. The advantage lies in ultra-high sensitivity and anti-background interference ability. Complementary effect: The colorimetric method provides preliminary qualitative results (e.g., color mutation indicates exceeding the standard), and the chemiluminescence method provides precise quantification. The combination of the two covers the full-process requirements from "coarse screening" to "precision measurement".

[0053] Through the dual-modal detection of the color reaction (change in the yellowness of the solution) and the chemiluminescence signal as described above, cross-verification of the nitrite concentration is achieved, greatly improving the detection accuracy. The potassium ferrocyanide reaction zone and the luminol chemiluminescence reaction zone are integrated in the microchannel, and the spatio-temporal separation of the two-stage reaction is realized through the control of the valve to avoid interference between reactants.

[0054] Example 2 Refer to Figures 9 - 24 , this example provides a microfluidic detection device for food additives, which can further realize the injection sampling of the solutions in each liquid storage pool and the control of the reaction process.

[0055] Specifically, it also includes a detection shell 400, the lower end of the detection shell 400 is fixedly connected to the support base 300, the upper side of the support base 300 is fixedly connected to a fixing frame 800, and the fixing frame 800 is connected to a movable seat that can move horizontally. The detection shell 400 is provided with a first injection assembly 600 for injecting a solution into the first microfluidic chip 100 and detecting the food additive to be detected, and a second injection assembly 700 for injecting a solution into each liquid reservoir in the second microfluidic chip 200 and detecting the food additive to be detected. The upper end of the detection shell 400 is fixedly connected to a top cover 500, and the first injection assembly 600 includes a first connection connected to the movable seat. The bracket 601 and a plurality of first syringes 607 connected to the lower end of the top cover 500 and corresponding to the first liquid storage tank 101d one by one, the first connecting bracket 601 is connected to a plurality of first injection brackets 602, the first injection bracket 602 is fixedly connected to a plurality of first lifting motors 603, the first injection bracket 602 below the first lifting motor 603 is slidably connected to a first injection connection block 605, the first injection connection block 605 is connected to a first injection liquid tube 606, the end of the first syringe 607 is connected to the first injection liquid tube 606 via a flexible pipe, when the first syringe 607 is actuated, the solution is pumped into the corresponding first injection liquid tube 606, and the first injection The injection bracket 602 is rotatably connected to a first injection screw rod 604 corresponding to the first lifting motor 603, the first injection connection block 605 is threadedly connected to the corresponding first injection screw rod 604, and the first injection liquid tube 606 can inject the solution into the corresponding first liquid storage tank 101d; the second injection assembly 700 includes a second connection bracket 701 connected to the movable seat and a plurality of second syringes 702 connected to the detection shell 400, the plurality of second syringes 702 and the plurality of liquid storage tanks in the second microfluidic chip 200 correspond one to one, the second connection bracket 701 is connected to a plurality of second injection brackets, and the second injection bracket is fixedly connected to a plurality of second injection brackets. Two lifting motors 703, a second injection connecting block 705 is slidably connected to the second injection bracket below the second lifting motor 703, a second injection connecting block 705 is connected to the second injection liquid tube 706, the end of the second syringe 702 is connected to the second injection liquid tube 706 via a flexible pipeline, when the second syringe 702 is actuated, the solution is pumped out into the corresponding second injection liquid tube 706; the second injection bracket is rotatably connected to the second injection screw 704 which corresponds to the second lifting motor 703 one by one, the second injection connecting block 705 is threadedly connected to the corresponding second injection screw 704, and the second injection liquid tube 706 can inject the solution into the corresponding second liquid storage tank.

[0056] Each first syringe 607 and each second syringe 702 stores the required analyte or detection solution respectively; when it is necessary to inject the required solution into each first liquid storage pool 101d of the first microfluidic chip 100, the first lifting motor 603 operates, the first injection lead screw 604 rotates, the first injection lead screw 604 drives the first injection connection block 605 to move downward, the first injection connection block 605 drives the first injection liquid pipe 606 to move downward, when the injection needle at the lower end of the first injection liquid pipe 606 is inserted into the corresponding first sample injection hole on the first upper chip body 102, the first lifting motor 603 stops operating, when the solution injected into each first liquid storage pool 101d reaches the required volume, the first syringe 607 stops operating, the first lifting motor 603 operates in the reverse direction to move the first injection connection block 605 upward to the initial position, and the first lifting motor 603 stops operating, thus realizing sample injection into the first microfluidic chip 100; when it is necessary to inject the required solution into each liquid storage pool of the second microfluidic chip 200, the second lifting motor 703 operates, the second injection lead screw 704 rotates, the second injection lead screw 704 drives the second injection connection block 705 to move downward, the second injection connection block 705 drives the second injection liquid pipe 706 to move downward, when the injection needle at the lower end of the second injection liquid pipe 706 is inserted into the corresponding second sample injection hole 203c on the second upper chip body 203, the second lifting motor 703 stops operating, when the solution injected into each second liquid storage pool reaches the required volume, the second syringe 702 stops operating, the second lifting motor 703 operates in the reverse direction to move the second injection connection block 705 upward to the initial position, and the second lifting motor 703 stops operating, thus realizing sample injection into the second microfluidic chip 200.

[0057] On the first upper chip body 102 at the first waste liquid pool 101b and the second waste liquid pool 203b101a, a first liquid extraction hole 102c and a second liquid extraction hole 102a are respectively opened. The first injection assembly 600 further includes a first peristaltic pump 625 and a second peristaltic pump fixedly connected inside the detection housing 400. A first hose and a second hose are respectively connected to the first peristaltic pump 625 and the second peristaltic pump. A first liquid extraction bracket is fixedly connected to the first connection bracket 601. A first liquid extraction vertical plate 633 and a second liquid extraction vertical plate 631 are connected to the first liquid extraction bracket. A first liquid extraction motor 632 is fixedly connected to the first liquid extraction vertical plate 633. A first liquid extraction lifting lead screw 626 is rotatably connected to the first liquid extraction vertical plate 633 below the first liquid extraction motor 632. A first liquid extraction connection block 634 slidably connected to the first liquid extraction vertical plate 633 is threadedly connected to the first liquid extraction lifting lead screw 626. A first liquid extraction tube 627 is fixedly connected to the first liquid extraction connection block 634. One end of the first liquid extraction tube 627 away from the first liquid extraction connection block 634 is connected to the first hose. A second liquid extraction motor 635 is fixedly connected to the second liquid extraction vertical plate 631. A second liquid extraction lifting lead screw 630 is rotatably connected to the second liquid extraction vertical plate 631 below the second liquid extraction motor 635. A second liquid extraction connection block 629 slidably connected to the second liquid extraction vertical plate 631 is threadedly connected to the second liquid extraction lifting lead screw 630. A second liquid extraction tube 628 is fixedly connected to the second liquid extraction connection block 629. One end of the second liquid extraction tube 628 away from the first liquid extraction connection block 634 is connected to the second hose. When it is necessary to make the solution in the liquid storage pool in the first microfluidic chip 100 flow in the direction of the first waste liquid pool 101b, the first liquid extraction motor 632 operates, the first liquid extraction lifting lead screw 626 rotates, driving the first liquid extraction connection block 634 to move downward. The tip at the bottom of the first liquid extraction tube 627 just inserts into the first liquid extraction hole 102c. The first liquid extraction motor 632 stops operating. The first peristaltic pump 625 operates, and through the first waste liquid pool 101b, the solution in the two left first liquid storage pools 101d flows in the direction of the first waste liquid pool 101b. After the reacted solution enters the first waste liquid pool 101b, the first peristaltic pump 625 stops operating. The first liquid extraction motor 632 operates in the reverse direction. When the first liquid extraction tube 627 is lifted away from the second microfluidic chip 200 to the initial height, the first liquid extraction motor 632 stops operating. When it is necessary to make the solution in the three first liquid storage pools 101d in the first microfluidic chip 100 flow in the direction of the second waste liquid pool 203b101a, the second liquid extraction motor 635 operates, the second liquid extraction lifting lead screw 630 rotates, driving the second liquid extraction connection block 629 to move downward. The tip at the bottom of the second liquid extraction tube 628 just inserts into the second liquid extraction hole 102a. The second liquid extraction motor 635 stops operating. The second peristaltic pump operates, and through the second waste liquid pool 203b101a, the solution in the three left first liquid storage pools 101d flows in the direction of the second waste liquid pool 203b101a;After the reacted solution enters the second waste liquid pool 203b101a, the second peristaltic pump stops operating, and the second liquid extraction motor 635 operates in the reverse direction. When the second liquid extraction pipe 628 is lifted away from the second microfluidic chip 200 to the initial height, the second liquid extraction motor 635 stops operating; when the solution flows to the required position and needs to stay for a period of time, controlling the corresponding peristaltic pump to operate is sufficient. This is prior art and will not be elaborated herein.

[0058] A third liquid extraction hole is formed on the second upper chip body 203 at the third waste liquid pool 202i. The second injection assembly 700 further includes a third peristaltic pump 716 fixedly connected inside the detection housing 400. A third hose is connected to the third peristaltic pump 716. A second liquid extraction bracket 722 is fixedly connected to the second connection bracket 701. A third liquid extraction vertical plate 721 is connected to the second liquid extraction bracket 722. A third liquid extraction motor 720 is fixedly connected to the third liquid extraction vertical plate 721. A third liquid extraction lifting lead screw 719 is rotatably connected to the third liquid extraction vertical plate 721 below the third liquid extraction motor 720. A third liquid extraction connection block 717 that is slidably connected to the third liquid extraction vertical plate 721 is threadedly connected to the third liquid extraction lifting lead screw 719. A third liquid extraction pipe 718 that can be aligned with the third liquid extraction hole is fixedly connected to the third liquid extraction connection block 717. One end of the third liquid extraction pipe 718 away from the third liquid extraction connection block 717 is connected to the third hose.

[0059] When it is necessary to make the solution at the end where the liquid storage pool is located flow in the direction of the third waste liquid pool 202i, control the third liquid extraction motor 720 to operate. The third liquid extraction lifting lead screw 719 rotates, driving the third liquid extraction connection block 717 to move. Control the operating direction of the third liquid extraction motor 720 to make the third liquid extraction connection block 717 move downward. When the tip at the bottom of the third liquid extraction pipe 718 just inserts into the third liquid extraction hole, the third liquid extraction motor 720 stops operating, and the third peristaltic pump 716 operates to make the solution in the liquid storage pool flow in the direction of the third waste liquid pool 202i. When it is necessary to make the solution stay in a certain channel for reaction, control the operation of the peristaltic pump to make the mixed and reacted solution stay in the set channel for reaction.

[0060] To further clamp the first microfluidic chip 100 and the second microfluidic chip 200, there are also two sets of clamping components 900 that can move horizontally and are respectively used to clamp the first microfluidic chip 100 and the second microfluidic chip 200. The clamping component 900 includes a clamping bottom plate 906 fixed in the detection housing 400. A clamping support base 907 that can slide left and right is connected to the upper side of the clamping bottom plate 906. A clamping movement motor is fixedly connected to the clamping bottom plate 906. A clamping movement lead screw 908 is rotatably connected to the clamping bottom plate 906. The clamping movement lead screw 908 is connected to the clamping movement motor. The clamping support base 907 is threadedly connected to the clamping movement lead screw 908. A clamping support plate 905 is fixedly connected to the upper side of the clamping support base 907. Clamping support parts are respectively fixedly connected to the front and rear ends of the clamping support plate 905. A clamping motor 901 is fixedly connected to the outside of one clamping support part. A clamping lead screw 902 that is rotatably connected between the two clamping support parts is connected to the clamping motor 901. A clamping movement block 903 is threadedly connected to the clamping lead screw 902. A connecting part 904 is fixedly connected to the upper side of the end of the clamping movement block 903 that extends outside the clamping support plate 905. A movable clamping plate 909 that slides along the upper side of the clamping support plate 905 is fixedly connected to the connecting part 904. A fixed clamping plate 905a that is arranged opposite to the movable clamping plate 909 is fixedly connected to the upper side of the clamping support plate 905. A first detection camera is fixedly connected to the fixed frame 800 at one end of the first injection component 600 in the left-right direction. A second detection camera is fixedly connected to the fixed frame 800 at one end of the second injection component 700 in the left-right direction. The first microfluidic chip 100 can be moved to below the first detection camera under the drive of the corresponding clamping seat. The second microfluidic chip 200 can be moved to below the second detection camera under the drive of the corresponding clamping seat.

[0061] Taking the clamping of the first microfluidic chip 100 as an example to illustrate the clamping process, place the first microfluidic chip 100 on the corresponding clamping support plate 905. The rear end of the first microfluidic chip 100 is attached to the front side of the fixed clamping plate 905a. Adjust the position of the first microfluidic chip 100 in the left-right direction so that the first liquid extraction hole 102c is aligned with the first liquid extraction tube 627 and the second liquid extraction hole 102a is aligned with the second liquid extraction tube 628. The clamping motor 901 operates, the clamping lead screw 902 rotates, the clamping movement block 903 slides, and drives the movable clamping plate 909 to move through the connecting part 904. Control the operation direction of the clamping motor 901 to make the movable clamping plate 909 move towards the direction where the first microfluidic chip 100 is located. When the movable clamping plate 909 abuts against the front side of the first microfluidic chip 100, the clamping motor 901 stops operating, realizing the clamping and fixing of the first microfluidic chip 100.

[0062] The process of clamping and fixing the second microfluidic chip 200 is similar to that of the first microfluidic chip 100 and will not be elaborated here.

[0063] Specifically, a first insertion hole 105a-1 is formed in the first moving transmission part 105a, a second insertion hole 104a-1 is formed in the second moving transmission part 104a, and a third insertion hole 103a-1 is formed in the third moving transmission part 103a. The first injection assembly 600 further includes a first upper connecting plate 612, which is fixedly connected to one end of the first connecting bracket 601 in the front-rear direction. A plurality of first guide rods 611 are arranged on the first upper connecting plate 612. A first lower connecting plate 610 is fixedly connected to the lower side of the first guide rods 611. A first driving motor is fixedly connected to the upper side of the first upper connecting plate 612. A first driving lead screw 613 rotatably connected between the first upper connecting plate 612 and the first lower connecting plate 610 is connected to the first driving motor. A first guide seat 609 sliding along the first guide rods 611 is threadedly connected to the first driving lead screw 613. A first moving lead screw 618, a second moving lead screw 619 and a third moving lead screw 620 are rotatably connected to the first guide seat 609. A first moving seat 623 sliding on the first guide seat 609 is threadedly connected to the first moving lead screw 618. A first moving rod 617 capable of being inserted into the first insertion hole 105a-1 is fixedly connected to the lower side of the first moving seat 623. A second moving seat 622 sliding on the second guide seat 707 is threadedly connected to the second moving lead screw 619. A second moving rod 616 capable of being inserted into the second insertion hole 104a-1 is fixedly connected to the lower side of the second moving seat 622. A third moving seat 624 sliding on the third guide seat is threadedly connected to the third moving lead screw 620. A third moving rod 616 capable of being inserted into the third insertion hole 103a-1 is fixedly connected to the lower side of the third moving seat 624. A first moving motor 608, a second moving motor 614 and a third moving motor 621 are fixedly connected to the outside of the first guide seat 609. The first moving lead screw 618 is in driving connection with the first moving motor 608, the second moving lead screw 619 is in driving connection with the second moving motor 614, and the third moving lead screw 620 is in driving connection with the third moving motor 621.

[0064] After the first microfluidic chip 100 is clamped and fixed, in the default initial state, each moving rod aligns with the corresponding insertion hole position; when the first microfluidic chip 100 is clamped and fixed, adjust the positions of the respective moving rods so that each moving rod aligns with the corresponding insertion hole, and then move each moving rod downward to insert it into the corresponding insertion hole to complete the preparatory operation for sample injection control. The specific action process is as follows: the first drive motor operates, the first drive lead screw 613 rotates, and the first guide seat 609 slides in the height direction, driving the first moving rod 617, the second moving rod 616, and the third moving rod 616 to move. Control the action direction of the first drive motor to move the three moving rods downward. When the first moving rod 617, the second moving rod 616, and the third moving rod 616 are respectively inserted into the set positions in the first insertion hole 105a-1, the second insertion hole 104a-1, and the third insertion hole 103a-1, the first drive motor stops operating; taking the adjustment of the positions of the first moving valve 105, the second moving valve 104, and the third moving valve 103 as an example to illustrate the solution flow control. When it is necessary to connect one end of two of the liquid outlet holes 105b-1 to two of the first storage pools 101d, control the first moving motor 608 to operate, the first moving lead screw 618 rotates, driving the first moving seat 623 to move, and driving the first moving valve 105 to move through the first moving rod 617. When one end of two of the liquid outlet holes 105b-1 is connected to two of the left first storage pools 101d, the first moving motor 608 stops operating, and the other ends of the two liquid outlet holes 105b-1 are connected to the first mixing channel 101i; control the second moving motor 614 to operate, the second moving lead screw 619 rotates, driving the second moving seat 622 to move, and driving the second moving valve 104 to move through the second moving rod 616. When one end of the first connecting mixing liquid hole 104b-1 is connected to the end of the first mixing channel 101i, the second moving motor 614 stops operating, and the other end of the first connecting mixing liquid hole 104b-1 is connected to the connecting channel 101c. Control the third moving motor 621 to operate, the third moving lead screw 620 rotates, driving the third moving seat 624 to move, and driving the third moving valve 103 to move through the third moving rod 616. When both ends of the first connecting waste liquid hole 103b-1 are respectively connected to the connecting channel 101c and the first waste liquid pool 101b, the third moving motor 621 stops operating, realizing the controllable connection between two of the left first storage pools 101d and the first waste liquid pool 101b; during the second-stage detection, control the first moving motor 608 to operate to connect the three liquid outlet holes 105b-1 to the three first storage pools 101d, and the first moving motor 608 stops operating;Control the operation of the second moving motor 614, and the second moving valve 104 moves. When both ends of the second connecting mixed liquid hole are respectively communicated with the end of the first mixing flow channel 101i and the second mixing flow channel 101g, the second moving motor 614 stops operating. Control the operation of the third moving motor 621, and the third moving valve 103 moves. When both ends of the second connecting waste liquid hole 103b-2 are respectively communicated with the end of the second mixing flow channel 101g and the second waste liquid tank 203b101a, the third moving motor 621 stops operating, realizing controllable sample injection in two detection methods.

[0065] Specifically, a fourth insertion hole 201b-1 is formed in the fourth moving transmission part 201b. The second injection assembly 700 further includes a second upper connecting plate 710. The second upper connecting plate 710 is fixedly connected to one end of the second connecting bracket 701 in the front-rear direction. A plurality of second guide rods 715 are arranged on the second upper connecting plate 710. A second lower connecting plate 711 is fixedly connected to the lower side of the second guide rods 715. A second driving motor 709 is fixedly connected to the upper side of the second upper connecting plate 710. A second driving lead screw rotatably connected between the second upper connecting plate 710 and the second lower connecting plate 711 is connected to the second driving motor 709. A second guide seat 707 sliding along the second guide rods 715 is threadedly connected to the second driving lead screw. A fourth moving lead screw 713 rotatably connected to the second guide seat 707 is rotatably connected to the second guide seat 707. A fourth moving motor 708 is fixedly connected to the outside of the second guide seat 707. The fourth moving motor 708 is connected to the fourth moving lead screw 713. A fourth moving seat 714 slidably connected to the second guide seat 707 is threadedly connected to the fourth moving lead screw 713. A fourth moving rod 712 capable of being inserted into the fourth insertion hole 201b-1 is fixedly connected to the lower side of the fourth moving seat 714.

[0066] After the second microfluidic chip 200 is clamped and fixed, in the default initial state, the fourth moving rod 712 is aligned with the position where the fourth insertion hole 201b-1 is located; after the second microfluidic chip 200 is clamped and fixed, the position of the fourth moving rod 712 is adjusted so that the fourth moving rod 712 is aligned with the fourth insertion hole 201b-1, and then the fourth moving rod 712 is moved downward and inserted into the fourth insertion hole 201b-1 to complete the preparatory operation for sample injection control. The specific operation process is as follows: the second driving motor 709 operates, the second driving lead screw rotates, the second driving lead screw drives the second guide seat 707 to move in the height direction, the second guide seat 707 drives the fourth moving rod 712 to move, and the operation direction of the second driving motor 709 is controlled to make the second guide seat 707 move downward. When the fourth moving rod 712 is inserted into the fourth moving transmission part 201b through the fourth insertion hole 201b-1, the second driving motor 709 stops operating; during detection, the fourth moving motor 708 operates, the fourth moving lead screw 713 rotates, driving the fourth moving seat 714 to move horizontally, and driving the fourth moving valve 201 to move through the fourth moving rod 712. When one ends of the two first liquid passing holes 201a-1 are respectively communicated with the antigen storage pool 202h and the first antibody storage pool 202g, the fourth moving motor 708 stops operating. When the antigen and antibody mixing reaction ends, the fourth moving motor 708 is controlled to operate. When the two ends of the second liquid passing hole 201a-2 are respectively communicated with the cleaning storage pool 202f and the head end of the third mixing channel 202b, the fourth moving motor 708 stops operating. The PBST in the cleaning storage pool 202f is extracted to clean the reaction layer. After the cleaning is completed, the fourth moving motor 708 is controlled to operate, and the fourth moving valve 201 moves. When the two ends of the second liquid passing hole 201a-2 are respectively communicated with the second antibody storage pool 202e and the head end of the third mixing channel 202b, the fourth moving motor 708 stops operating. After the incubation is completed, the fourth moving motor 708 is controlled to operate. When the two ends of the second liquid passing hole 201a-2 are respectively communicated with the cleaning storage pool 202f and the head end of the third mixing channel 202b, the fourth moving motor 708 stops operating. The PBST in the cleaning storage pool 202f is extracted to clean the reaction layer. After the cleaning is completed, the fourth moving motor 708 is controlled to operate, and the fourth moving valve 201 moves. When the two ends of the second liquid passing hole 201a-2 are respectively communicated with the luminescent substrate storage pool 202d and the third mixing channel 202b, the fourth moving motor 708 stops operating, realizing the controllable flow injection of the analyte and the detection solution.

[0067] When the solution reaction in the microfluidic chip ends and it is necessary to detect the content of the analyte, the clamping and moving motor is controlled to operate, the clamping and moving lead screw 908 rotates, the clamping and moving lead screw 908 drives the clamping support seat 907 to slide left and right along the clamping bottom plate 906, and the clamping support seat 907 drives the corresponding microfluidic chip to move through the clamping support plate 905. When the waste liquid pool moves below the corresponding detection camera, the clamping and moving motor stops operating.

[0068] The fixed clamping plate 905a corresponding to the first microfluidic chip 100 and the fixed clamping plate 905a corresponding to the second microfluidic chip 200 are respectively referred to as the first fixed clamping plate 905a and the second fixed clamping plate 905a. Three first guiding holes 905a-2 corresponding to the first moving transmission part 105a, the second moving transmission part 104a, and the third moving transmission part 103a are respectively formed on the first fixed clamping plate 905a. The first moving transmission part 105a, the second moving transmission part 104a, and the third moving transmission part 103a are respectively slidably connected to the first fixed clamping plate 905a through the first guiding holes 905a-2, and a plurality of insertion holes are always behind the fixed clamping plate 905a. A second guiding hole 905a-1 corresponding to the fourth moving transmission part 201b is formed on the second fixed clamping plate 905a, and the fourth moving transmission part 201b is slidably connected to the second fixed clamping plate 905a through the second guiding hole 905a-1.

[0069] With the above structure, the stability during the position adjustment of each moving valve is improved.

[0070] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention, and they should all be covered by the scope of the claims of the present invention.

Claims

1. A microfluidic detection device for food additives, characterized in that: These include, A first microfluidic chip comprises a first upper chip body and a first lower chip body fixed to the lower side of the first upper chip body, wherein a plurality of first liquid reservoirs, a plurality of liquid outlet channels corresponding to the first liquid reservoirs, a first mixing channel, a second mixing channel, a first waste liquid pool and a second waste liquid pool are arranged at an upward end of the first lower chip body, one end of the liquid outlet channel is connected to the first liquid reservoir, the other end of the liquid outlet channel is controllably connected to one end of the first mixing channel or one end of the second mixing channel, and the other end of the second mixing channel is connected to the second waste liquid pool; The second microfluidic chip comprises a second upper chip body and a second lower chip body fixed on the lower side of the second upper chip body, wherein an antigen reservoir, a first antibody reservoir, a cleaning reservoir, a second antibody reservoir, a luminescent substrate reservoir and a third mixing channel are provided at an upward end of the second lower chip body, the antigen reservoir, the first antibody reservoir, the cleaning reservoir, the second antibody reservoir and the luminescent substrate reservoir are controllably connected to one end of the third mixing channel, a microchannel is provided at an upward end of the second lower chip body at the other end of the third mixing channel, a silica gel membrane coated with an antigen is sandwiched between the second upper chip body and the second lower chip body at the microchannel, and a third waste liquid tank is provided at an upward end of the second lower chip body away from the third mixing channel.

2. The microfluidic detection device for food additives according to claim 1, characterized in that: The first microfluidic chip also includes a first movable valve, a second movable valve and a third movable valve. A first movable channel is opened on the upward end of the first lower chip body between the plurality of liquid outlet channels and the first mixing channel or the second mixing channel. The first movable valve can just slide along the first movable channel. A second movable channel is opened on the first lower chip body between the first mixing channel and the first waste liquid pool. The second movable valve can just slide along the second movable channel. A third movable channel is opened on the first lower chip body between the first mixing channel and the first waste liquid pool and between the second mixing channel and the second waste liquid pool. The third movable valve can just slide along the third movable channel. The first movable valve enables the plurality of liquid storage pools to be controllably connected to the first mixing channel or the second mixing channel. The second movable valve enables the first mixing channel to be connected to the first waste liquid pool or the other end of the first mixing channel to be connected to the second mixing channel. The third movable valve enables the other end of the first mixing channel to be connected to the first waste liquid pool or the end of the second mixing channel to be connected to the second waste liquid pool.

3. The microfluidic detection device for food additives according to claim 2, characterized in that: The first movable valve includes a first movable transmission part outside the first lower chip body, a first movable part just inserted into the first movable channel is fixed on the first movable transmission part, a plurality of liquid outlet holes corresponding to the first liquid storage tanks are arranged on the first movable part, and three first liquid storage tanks are provided. When one end of two of the liquid outlet holes is connected with two of the first liquid storage tanks, the other ends of the two liquid outlet holes are connected with the first mixing channel; when one end of the three liquid outlet holes is connected with the three first liquid storage tanks one by one, the other ends of two of the liquid outlet holes are connected with the first mixing channel, and the other end of another liquid outlet hole is connected with the head end of the second mixing channel.

4. The microfluidic detection device for food additives according to claim 2, characterized in that: The second movable valve includes a second movable transmission part outside the first lower chip body, a second movable part just inserted into the second movable channel is fixed on the second movable transmission part, and a first connecting mixed liquid hole and a second connecting mixed liquid hole are opened on the second movable part. When one end of the first connecting mixed liquid hole is connected to the end of the first mixing channel, the other end of the first connecting mixed liquid hole is connected to the first waste liquid pool, and the second connecting mixed liquid hole and any mixing channel or waste liquid pool are staggered. When one end of the second connecting mixed liquid hole is connected to the end of the first mixing channel, the other end of the second connecting mixed liquid hole is connected to the second mixing channel.

5. The microfluidic detection device for food additives according to claim 2, characterized in that: The third movable valve includes a third movable transmission part outside the first lower chip body, and a third movable part just inserted into the third movable channel is fixed on the third movable transmission part. The third movable part is provided with a first waste liquid connection hole and a second waste liquid connection hole. When the two ends of the first waste liquid connection hole are respectively connected to the first mixing channel and the first waste liquid pool, the two ends of the second waste liquid connection hole are respectively staggered with the second mixing channel and the second waste liquid pool; when the two ends of the second waste liquid connection hole are respectively connected to the second mixing channel and the second waste liquid pool, the two ends of the first waste liquid connection hole are respectively staggered with the second mixing channel and the second waste liquid pool.

6. The microfluidic detection device for food additives according to claim 1, characterized in that: A fourth moving channel is provided on the second lower chip body between the liquid reservoir and the third mixing channel, the second microfluidic chip further comprises a fourth moving valve, the fourth moving valve comprises a fourth moving transmission part outside the second lower chip body, the fourth moving transmission part comprises a fourth plug hole, a fourth moving part just inserted into the fourth moving channel is fixed on the fourth moving transmission part, two first liquid holes and one second liquid hole are provided on the fourth moving part, when one end of the two first liquid holes are respectively connected to the antigen liquid reservoir and the first antibody liquid reservoir, the other end of the first liquid hole is connected to one end of the third mixing channel, and the second liquid hole is staggered with any other liquid reservoir; When one end of the second liquid through hole is connected to the cleaning reservoir or the second antibody reservoir or the luminescent substrate reservoir, the other end of the second liquid through hole is connected to one end of the third mixing channel, and the two first liquid through holes are staggered with any other reservoir.

7. The microfluidic detection device for food additives according to claim 4, characterized in that: It also includes a detection shell, in which a fixed frame is fixedly connected, and a movable seat capable of horizontal movement is connected to the fixed frame, and a first injection component for injecting solution into the first microfluidic chip and detecting food additives to be detected and a second injection component for injecting solution into each liquid reservoir in the second microfluidic chip and detecting food additives to be detected are provided on the movable seat, the first injection component includes a first connecting bracket connected to the movable seat, a plurality of first injection brackets are connected to the first connecting bracket, a plurality of first lifting motors are fixedly connected to the first injection bracket, a first injection connecting block is slidably connected to the first injection bracket below the first lifting motor, a first injection liquid tube is connected to the first injection connecting block, and a first injection bracket is rotatably connected to the first lifting motor. A one-to-one corresponding first injection screw, the first injection connecting block is threadedly connected to the corresponding first injection screw, and the first injection liquid tube can inject the solution into the corresponding first liquid storage tank; the second injection assembly includes a second connecting bracket connected to the movable seat, the second connecting bracket is connected to a plurality of second injection brackets, the second injection bracket is fixedly connected to a plurality of second lifting motors, the second injection bracket under the second lifting motor is slidably connected to a second injection connecting block, the second injection connecting block is connected to a second injection liquid tube, the second injection bracket is rotatably connected to a second injection screw corresponding to the second lifting motor, the second injection connecting block is threadedly connected to the corresponding second injection screw, and the second injection liquid tube can inject the solution into the corresponding second liquid storage tank.

8. The microfluidic detection device for food additives according to claim 7, characterized in that: The invention also includes two groups of clamping components that can move horizontally and are used to clamp the first microfluidic chip and the second microfluidic chip respectively, the clamping component includes a clamping base plate fixed in the detection shell, the upper side of the clamping base plate is connected to a clamping support seat that can slide left and right, the clamping base plate is fixedly connected to a clamping moving motor, the clamping base plate is rotatably connected to a clamping moving screw rod, the clamping moving screw rod is connected to the clamping moving motor, the clamping support seat is threadedly connected to the clamping moving screw rod, the upper side of the clamping support seat is fixedly connected to a clamping support plate, the front and rear ends of the clamping support plate are respectively fixedly connected to clamping support parts, the outer side of a clamping support part is fixedly connected to a clamping motor, and the clamping motor is connected to a rotatable connection between the two clamping A clamping screw between the supporting parts, a clamping moving block is threadedly connected to the clamping screw, a connecting part is fixedly connected to the upper side of one end of the clamping moving block extending out of the clamping support plate, a movable clamping plate which slides along the upper side of the clamping support plate is fixedly connected to the connecting part, a fixed clamping plate which is arranged opposite to the movable clamping plate is fixedly connected to the upper side of the clamping support plate, a first detection camera is fixedly connected to a fixed frame at one end of the first injection assembly in the left and right directions, a second detection camera is fixedly connected to a fixed frame at one end of the second injection assembly in the left and right directions, the first microfluidic chip can be moved to the bottom of the first detection camera under the drive of the corresponding clamping seat, and the second microfluidic chip can be moved to the bottom of the second detection camera under the drive of the corresponding clamping seat.

9. The microfluidic detection device for food additives according to claim 3, characterized in that: The first movable transmission part has a first plug hole, the second movable transmission part has a second plug hole, the third movable transmission part has a third plug hole, the first injection assembly also includes a first upper connecting plate, the first upper connecting plate is fixedly connected to one end of the first connecting bracket in the front-to-back direction, a plurality of first guide rods are arranged on the first upper connecting plate, a first lower connecting plate is fixedly connected to the lower side of the first guide rod, a first transmission motor is fixedly connected to the upper side of the first upper connecting plate, a first transmission screw rod rotatably connected between the first upper connecting plate and the first lower connecting plate is connected to the first transmission motor, a screw rod is threadedly connected to the first transmission screw rod which slides along the first guide rod The first guide seat, the first guide seat is rotatably connected with a first movable screw rod, a second movable screw rod and a third movable screw rod, the first movable screw rod is threadedly connected with a first movable seat slidably connected to the first guide seat, the lower side of the first movable seat is fixedly connected with a first movable rod capable of being inserted into the first plug hole, the second movable screw rod is threadedly connected with a second movable seat slidably connected to the second guide seat, the lower side of the second movable seat is fixedly connected with a second movable rod capable of being inserted into the second plug hole, the third movable screw rod is threadedly connected with a third movable seat slidably connected to the third guide seat, and the lower side of the third movable seat is fixedly connected with a third movable rod capable of being inserted into the third plug hole.

10. The microfluidic detection device for food additives according to claim 6, characterized in that: The fourth movable transmission part is provided with a fourth plugging hole, the second injection assembly also includes a second upper connecting plate, the second upper connecting plate is fixedly connected to one end of the second connecting bracket in the front-to-back direction, a plurality of second guide rods are arranged on the second upper connecting plate, the second guide rod is fixedly connected to the second lower connecting plate at the lower side, the second upper connecting plate is fixedly connected to the second transmission motor at the upper side, the second transmission motor is connected with a second transmission screw rod rotatably connected between the second upper connecting plate and the second lower connecting plate, the second transmission screw rod is threadedly connected with a second guide seat which slides along the second guide rod, the second guide seat is rotatably connected with a fourth movable screw rod, the fourth movable screw rod is threadedly connected with a fourth movable seat which is slidably connected to the second guide seat, and the fourth movable rod which can be inserted into the fourth plugging hole is fixedly connected to the lower side of the fourth movable seat.

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