Earthworm protein fertilizer, preparation method and application of earthworm protein fertilizer in betel nuts

Abstract

The invention belongs to the technical field of preparation of earthworm protein fertilizers, and particularly relates to an earthworm protein fertilizer, a preparation method and application of the earthworm protein fertilizer in betel nuts. According to the method, the fresh earthworms are subjected to ultrasonic treatment and freezing treatment, so that beneficial components in the earthworms can be secreted and stored; phytase, ligninase, hemicellulase and the like are used for carrying out enzymolysis on the earthworms, so that effective components in the earthworms can be further separated out, and the effective active components in the fertilizer are enriched. Therefore, the fertilizer prepared by specifically compounding the earthworm protein enzymatic hydrolysate, the betel nut shell powder, the rice straw, the potassium chloride, the calcium magnesium phosphate fertilizer and the urea is high in nutritional value, and when the fertilizer is applied to the betel nuts, the yield of the betel nuts can be remarkably increased, and healthy development of the betel nut industry is protected.

Description

Technical Field

[0001] The present invention belongs to the technical field of earthworm protein fertilizer preparation, and specifically relates to an earthworm protein fertilizer, a preparation method thereof, and its application in areca nut. Background Art

[0002] Earthworms, commonly known as earth dragons and also known as eels, are invertebrates belonging to the phylum Annelida and are widely distributed in soils around the world. Earthworms have the characteristics of strong adaptability, wide distribution, extensive diet, large food intake, low susceptibility to disease, and high reproductive rate. They play an important role in the ecosystem. At the same time, earthworms contain rich proteins and amino acids, so they have high market application value.

[0003] There are relevant reports on earthworm fertilizers and their applications in the prior art, but the target objects of earthworm fertilizers obtained by different preparation methods have certain differences. For example, in Chinese Patent CN116023191A, an earthworm liquid fertilizer was prepared by enzymolysis and fermentation of earthworms using trypsin, which can specifically increase the yield of strawberries; in Chinese Patent CN110800760A, an earthworm hydrolysate prepared by hydrolyzing earthworms is beneficial to reducing the occurrence of tomato diseases. It can be seen that the preparation method is closely related to the application object of earthworm fertilizers. However, there is no report on the use of earthworm fertilizers in areca nut at present. Summary of the Invention

[0004] The purpose of the present invention is to provide an earthworm protein fertilizer, a preparation method thereof, and its application in areca nut. This earthworm protein fertilizer can specifically act on areca nut and has the characteristic of increasing the yield of areca nut.

[0005] The present invention provides an earthworm protein fertilizer, which includes: earthworm protease hydrolysate, areca nut shell powder, rice straw, potassium chloride, calcium magnesium phosphate fertilizer, and urea;

[0006] The preparation method of the earthworm protease hydrolysate includes:

[0007] Fresh earthworms are sequentially subjected to ultrasonic treatment, freezing, and pulverization to obtain pulverized earthworms;

[0008] The pulverized earthworms, water, and complex enzyme are mixed for enzymolysis to obtain an earthworm protease hydrolysate;

[0009] The complex enzyme includes: phytase, ligninase, and hemicellulase.

[0010] Preferably, by weight, the earthworm protein fertilizer includes:

[0011] 10 - 15 parts of earthworm protease hydrolysate, 23 - 28 parts of betel nut shell powder, 18 - 20 parts of rice straw, 5 - 8 parts of potassium chloride, 7 - 12 parts of calcium magnesium phosphate fertilizer, and 10 - 15 parts of urea.

[0012] Preferably, the mass ratio of the phytase, ligninase, and hemicellulase is 0.2 - 0.5:1:1.2 - 1.4.

[0013] Preferably, the mass ratio of the pulverized earthworms, water, and complex enzyme is 1:1 - 1.5:0.4 - 0.6.

[0014] Preferably, the time for the mixed enzymolysis is 70 - 85 min; the temperature for the mixed enzymolysis is 50 - 55 °C.

[0015] Preferably, the frequency of the ultrasound is 150 - 180 w; the time of the ultrasound is 35 - 45 min;

[0016] The temperature of the freezing is -25 - -35 °C; the time of the freezing is 50 - 60 min.

[0017] The present invention provides a preparation method of the earthworm protein fertilizer according to the above technical solution, including the following steps:

[0018] Mix the betel nut shell powder, rice straw, complex bacteria, and earthworm protease hydrolysate, and then perform composting treatment to obtain a fermented product;

[0019] Mix the fermented product, potassium chloride, calcium magnesium phosphate fertilizer, and urea evenly to obtain the earthworm protein fertilizer;

[0020] The complex bacteria include: arbuscular mycorrhizal fungi, Klebsiella, and Bacillus mucilaginosus.

[0021] Preferably, the effective viable counts of the arbuscular mycorrhizal fungi, Klebsiella, and Bacillus mucilaginosus are respectively ≥ 0.5×10 7 cfu / mL;

[0022] The volume ratio of the arbuscular mycorrhizal fungi, Klebsiella, and Bacillus mucilaginosus is 1:2 - 2.5:2.

[0023] Preferably, when performing composting, based on the total mass of the betel nut shell powder, rice straw, and earthworm protease hydrolysate, the added volume of the complex bacteria and the total mass ratio of the betel nut shell powder, rice straw, and earthworm protease hydrolysate is 0.5 - 0.8 mL:10 g;

[0024] The time for the composting treatment is 8 - 12 d; the temperature for the composting treatment is 45 - 60 °C.

[0025] The present invention provides the application of the earthworm protein fertilizer described in the above technical solution in increasing the yield of areca nut.

[0026] Beneficial effects:

[0027] The present invention provides an earthworm protein fertilizer, which includes: earthworm protease hydrolysate, areca nut shell powder, rice straw, potassium chloride, calcium magnesium phosphate fertilizer and urea. By subjecting fresh earthworms to ultrasonic treatment and freezing treatment, it is beneficial for the secretion and preservation of beneficial components in the earthworms; then using phytase, ligninase, hemicellulase, etc. to enzymatically hydrolyze the earthworms, which is beneficial for the further precipitation of effective components in the earthworms and enriches the effective active components in the fertilizer. Therefore, the fertilizer prepared by specifically compounding earthworm protease hydrolysate, areca nut shell powder, rice straw, potassium chloride, calcium magnesium phosphate fertilizer and urea has the characteristics of high nutritional value and good application effect.

[0028] Based on the above technical advantages, the present invention also provides a preparation method of the earthworm protein fertilizer described in the above technical solution and its application in areca nut. By applying the earthworm protein fertilizer provided by the present invention to areca nut, the yield of areca nut can be significantly increased. Specific embodiments

[0029] In the present invention, unless otherwise specified, the raw materials, equipment and steps used are all conventional selections.

[0030] To further illustrate the present invention, the following describes the solution provided by the present invention in detail with reference to the examples, but they should not be construed as limiting the protection scope of the present invention.

[0031] Example 1

[0032] A preparation method of an earthworm protein fertilizer, the steps are as follows:

[0033] (1) Prepare earthworm protease hydrolysate:

[0034] Under the environment with a frequency of 180w, ultrasonically treat fresh earthworms for 40 min to obtain ultrasonically treated earthworms; then freeze the ultrasonically treated earthworms in an environment of -30 °C for 60 min to obtain frozen earthworms, and crush the frozen earthworms to obtain crushed earthworms;

[0035] Mix and enzymatically hydrolyze the crushed earthworms, water and complex enzyme (the complex enzyme is obtained by mixing phytase, ligninase and hemicellulase according to the mass ratio of 0.2:1:1.2) according to the mass ratio of 1:1.5:0.4, the enzymolysis time is 70 min, the enzymolysis temperature is 52 °C, and stirring is carried out during the enzymolysis process, and the stirring speed is 150 rpm, so as to obtain earthworm protease hydrolysate;

[0036] (2) By weight, the earthworm protein fertilizer includes: 10 parts of the earthworm protease hydrolysate in step (1), 23 parts of betel nut shell powder, 18 parts of rice straw, 8 parts of potassium chloride, 12 parts of calcium magnesium phosphate fertilizer, and 15 parts of urea; the rice straw is the pulverized rice straw;

[0037] (3) According to the mass parts in step (2), mix the betel nut shell powder, rice straw, and earthworm protease hydrolysate, and then add a composite strain (the composite strain is composed of arbuscular mycorrhizal fungi, Klebsiella, and Bacillus mucilaginosus in a mass ratio of 1:2:2, and the effective viable count of arbuscular mycorrhizal fungi, Klebsiella, and Bacillus mucilaginosus is 0.5×10 7 cfu / mL), and perform composting treatment at 60 °C for 10 d to obtain a fermented product; among them, the volume ratio of the total mass of the betel nut shell powder, rice straw, and earthworm protease hydrolysate to the composite strain is 10 g:0.5 mL;

[0038] Mix the above fermented product, potassium chloride, calcium magnesium phosphate fertilizer, and urea evenly to obtain the earthworm protein fertilizer.

[0039] Example 2

[0040] A preparation method of an earthworm protein fertilizer, the steps are as follows:

[0041] (1) Prepare an earthworm protease hydrolysate:

[0042] Under the environment with a frequency of 150 w, ultrasonically treat fresh earthworms for 40 min to obtain ultrasonically treated earthworms; then freeze the ultrasonically treated earthworms in an environment of -30 °C for 60 min to obtain frozen earthworms, and pulverize the frozen earthworms to obtain pulverized earthworms;

[0043] Mix the pulverized earthworms, water, and a composite enzyme (the composite enzyme is obtained by mixing phytase, ligninase, and hemicellulase in a mass ratio of 0.2:1:1.2) in a mass ratio of 1:1.5:0.4 for enzymatic hydrolysis. The enzymatic hydrolysis time is 70 min, the enzymatic hydrolysis temperature is 53 °C, and stirring is accompanied during the enzymatic hydrolysis process. The stirring speed is 150 rpm, and then an earthworm protease hydrolysate is obtained;

[0044] (2) By weight, the earthworm protein fertilizer includes: 10 parts of the earthworm protease hydrolysate in step (1), 25 parts of betel nut shell powder, 20 parts of rice straw, 8 parts of potassium chloride, 12 parts of calcium magnesium phosphate fertilizer, and 13 parts of urea; the rice straw is the pulverized rice straw;

[0045] (3) According to the parts by mass in step (2), mix areca nut shell powder, rice straw, and earthworm protease hydrolysate, and then add a composite strain (the composite strain is composed of arbuscular mycorrhizal fungi, Klebsiella, and Bacillus mucilaginosus in a mass ratio of 1:2:2, and the effective viable count of arbuscular mycorrhizal fungi, Klebsiella, and Bacillus mucilaginosus is 0.5×10 7 cfu / mL), and perform composting treatment at 60°C for 10 days to obtain a fermented product; among them, the total mass of areca nut shell powder, rice straw, and earthworm protease hydrolysate and the volume ratio of the composite strain is 10 g:0.5 mL;

[0046] Mix the above fermented product, potassium chloride, calcium magnesium phosphate fertilizer, and urea evenly to obtain earthworm protein fertilizer.

[0047] Comparative Example 1

[0048] A method for preparing earthworm protein fertilizer, the steps are as follows:

[0049] (1) Prepare earthworm protease hydrolysate:

[0050] Crush fresh earthworms to obtain crushed earthworms;

[0051] Mix the crushed earthworms, water, and a composite enzyme (the composite enzyme is obtained by mixing phytase, ligninase, and hemicellulase in a mass ratio of 0.2:1:1.2) in a mass ratio of 1:1.5:0.4 for enzymatic hydrolysis. The enzymatic hydrolysis time is 70 min, the enzymatic hydrolysis temperature is 52°C, and stirring is carried out during the enzymatic hydrolysis process. The stirring speed is 150 rpm, and then earthworm protease hydrolysate is obtained;

[0052] (2) By weight, the earthworm protein fertilizer includes: 10 parts of the earthworm protease hydrolysate in step (1), 23 parts of areca nut shell powder, 18 parts of rice straw, 8 parts of potassium chloride, 12 parts of calcium magnesium phosphate fertilizer, and 15 parts of urea; among them, the rice straw is crushed rice straw;

[0053] (3) According to the parts by mass in step (2), mix areca nut shell powder, rice straw, and earthworm protease hydrolysate, and then add a composite strain (the composite strain is composed of arbuscular mycorrhizal fungi, Klebsiella, and Bacillus mucilaginosus in a mass ratio of 1:2:2, and the effective viable count of arbuscular mycorrhizal fungi, Klebsiella, and Bacillus mucilaginosus is 0.5×10 7 cfu / mL), and perform composting treatment at 60°C for 10 days to obtain a fermented product; among them, the total mass of areca nut shell powder, rice straw, and earthworm protease hydrolysate and the volume ratio of the composite strain is 10 g:0.5 mL;

[0054] Mix the above fermented product, potassium chloride, calcium magnesium phosphate fertilizer, and urea evenly to obtain earthworm protein fertilizer.

[0055] Comparative Example 2

[0056] A preparation method of an earthworm protein fertilizer, the steps are as follows:

[0057] (1) Prepare an earthworm protease hydrolysate:

[0058] Under the environment with a frequency of 180w, ultrasonically treat fresh earthworms for 40min to obtain ultrasonically treated earthworms; then freeze the ultrasonically treated earthworms in an environment of -30°C for 60min to obtain frozen earthworms, and crush the frozen earthworms to obtain crushed earthworms;

[0059] Mix the crushed earthworms, water and a composite enzyme (the composite enzyme is obtained by mixing phytase, ligninase and hemicellulase according to a mass ratio of 1:1:1.2) according to a mass ratio of 1:1.5:0.4 for enzymatic hydrolysis. The enzymatic hydrolysis time is 70min, the enzymatic hydrolysis temperature is 52°C, and stirring is carried out during the enzymatic hydrolysis process. The stirring speed is 150rpm, and then an earthworm protease hydrolysate is obtained;

[0060] (2) By weight, the earthworm protein fertilizer includes: 10 parts of the earthworm protease hydrolysate in step (1), 23 parts of betel nut shell powder, 18 parts of rice straw, 8 parts of potassium chloride, 12 parts of calcium magnesium phosphate fertilizer and 15 parts of urea; wherein the rice straw is crushed rice straw;

[0061] (3) According to the mass parts in step (2), mix the betel nut shell powder, rice straw and the earthworm protease hydrolysate, and then add a composite strain (the composite strain is composed of arbuscular mycorrhizal fungi, Klebsiella and Bacillus mucilaginosus according to a mass ratio of 1:2:2, and the effective viable counts of arbuscular mycorrhizal fungi, Klebsiella and Bacillus mucilaginosus are all 0.5×10 7 cfu / mL), and carry out composting treatment at 60°C for 10d to obtain a fermented product; wherein, the total mass of the betel nut shell powder, rice straw and the earthworm protease hydrolysate and the volume ratio of the composite strain are 10g:0.5mL;

[0062] Mix the above fermented product, potassium chloride, calcium magnesium phosphate fertilizer and urea evenly to obtain an earthworm protein fertilizer.

[0063] Comparative Example 3

[0064] A preparation method of an earthworm protein fertilizer, the steps are as follows:

[0065] (1) Prepare an earthworm protease hydrolysate:

[0066] Under the environment with a frequency of 180w, fresh earthworms were ultrasonically treated for 40 min to obtain ultrasonically treated earthworms; then the ultrasonically treated earthworms were frozen in an environment of -30°C for 60 min to obtain frozen earthworms, and the frozen earthworms were pulverized to obtain pulverized earthworms;

[0067] The pulverized earthworms, water and ligninase were mixed for enzymatic hydrolysis according to a mass ratio of 1:1.5:0.4. The enzymatic hydrolysis time was 70 min, the enzymatic hydrolysis temperature was 52°C, and stirring was carried out during the enzymatic hydrolysis process at a stirring speed of 150 rpm, thereby obtaining earthworm protease hydrolysate;

[0068] (2) By weight, the earthworm protein fertilizer includes: 10 parts of the earthworm protease hydrolysate in step (1), 23 parts of betel nut shell powder, 18 parts of rice straw, 8 parts of potassium chloride, 12 parts of calcium magnesium phosphate fertilizer and 15 parts of urea; where the rice straw is pulverized rice straw;

[0069] (3) According to the mass parts in step (2), the betel nut shell powder, rice straw and earthworm protease hydrolysate were mixed and then composite strains were added (the composite strains are composed of arbuscular mycorrhizal fungi, Klebsiella and Bacillus mucilaginosus in a mass ratio of 1:2:2, and the effective viable counts of arbuscular mycorrhizal fungi, Klebsiella and Bacillus mucilaginosus are all 0.5×10 7 cfu / mL), and composting treatment was carried out at 60°C for 10 d to obtain a fermented product; among them, the total mass of the betel nut shell powder, rice straw and earthworm protease hydrolysate and the volume ratio of the composite strains is 10 g:0.5 mL;

[0070] The above fermented product, potassium chloride, calcium magnesium phosphate fertilizer and urea were mixed evenly to obtain the earthworm protein fertilizer.

[0071] Control 4

[0072] A preparation method of an earthworm protein fertilizer, the steps are as follows:

[0073] (1) Preparation of earthworm protease hydrolysate:

[0074] Under the environment with a frequency of 180w, fresh earthworms were ultrasonically treated for 40 min to obtain ultrasonically treated earthworms; then the ultrasonically treated earthworms were frozen in an environment of -30°C for 60 min to obtain frozen earthworms, and the frozen earthworms were pulverized to obtain pulverized earthworms;

[0075] Mix the crushed earthworms, water, and composite enzyme (the composite enzyme is obtained by mixing phytase, ligninase, and hemicellulase in a mass ratio of 0.2:1:1.2) in a mass ratio of 1:1.5:0.8 for enzymatic hydrolysis. The enzymatic hydrolysis time is 70 min, the enzymatic hydrolysis temperature is 60 °C, and stirring is carried out during the enzymatic hydrolysis process at a stirring speed of 150 rpm to obtain an earthworm protein enzymatic hydrolysate;

[0076] (2) By weight, the earthworm protein fertilizer includes: 10 parts of the earthworm protein enzymatic hydrolysate in step (1), 23 parts of betel nut shell powder, 18 parts of rice straw, 8 parts of potassium chloride, 12 parts of calcium magnesium phosphate fertilizer, and 15 parts of urea; wherein the rice straw is crushed rice straw;

[0077] (3) According to the mass parts in step (2), mix the betel nut shell powder, rice straw, and earthworm protein enzymatic hydrolysate, and then add a composite strain (the composite strain is composed of arbuscular mycorrhizal fungi, Klebsiella, and Bacillus mucilaginosus in a mass ratio of 1:2:2, and the effective viable count of arbuscular mycorrhizal fungi, Klebsiella, and Bacillus mucilaginosus is 0.5×10 7 cfu / mL), and perform composting treatment at 60 °C for 10 d to obtain a fermented product; wherein, the volume ratio of the total mass of the betel nut shell powder, rice straw, and earthworm protein enzymatic hydrolysate to the composite strain is 10 g:0.5 mL;

[0078] Mix the above fermented product, potassium chloride, calcium magnesium phosphate fertilizer, and urea evenly to obtain the earthworm protein fertilizer.

[0079] Control Example 5

[0080] A preparation method of an earthworm protein fertilizer, the steps are as follows:

[0081] (1) Prepare an earthworm protein enzymatic hydrolysate:

[0082] Under an environment with a frequency of 180 w, ultrasonically treat fresh earthworms for 40 min to obtain ultrasonically treated earthworms; then freeze the ultrasonically treated earthworms in an environment of -30 °C for 60 min to obtain frozen earthworms, and crush the frozen earthworms to obtain crushed earthworms;

[0083] Mix the crushed earthworms, water, and composite enzyme (the composite enzyme is obtained by mixing phytase, ligninase, and hemicellulase in a mass ratio of 0.2:1:1.2) in a mass ratio of 1:1.5:0.4 for enzymatic hydrolysis. The enzymatic hydrolysis time is 70 min, the enzymatic hydrolysis temperature is 52 °C, and stirring is carried out during the enzymatic hydrolysis process at a stirring speed of 150 rpm to obtain an earthworm protein enzymatic hydrolysate;

[0084] (2) By weight, the earthworm protein fertilizer includes: 20 parts of the earthworm protease hydrolysate in step (1), 23 parts of betel nut shell powder, 18 parts of rice straw, 8 parts of potassium chloride, 12 parts of calcium magnesium phosphate fertilizer, and 15 parts of urea; the rice straw is the pulverized rice straw;

[0085] (3) According to the mass parts in step (2), mix the betel nut shell powder, rice straw, and earthworm protease hydrolysate, and then add a composite strain (the composite strain is composed of arbuscular mycorrhizal fungi, Klebsiella, and Bacillus mucilaginosus in a mass ratio of 1:2:2, and the effective viable count of arbuscular mycorrhizal fungi, Klebsiella, and Bacillus mucilaginosus is 0.5×10 7 cfu / mL), and perform composting treatment at 60°C for 10 d to obtain a fermented product; among them, the volume ratio of the total mass of the betel nut shell powder, rice straw, and earthworm protease hydrolysate to the composite strain is 10 g:0.5 mL;

[0086] Mix the above fermented product, potassium chloride, calcium magnesium phosphate fertilizer, and urea evenly to obtain the earthworm protein fertilizer.

[0087] Comparative Example 6

[0088] A preparation method of an earthworm protein fertilizer, the steps are as follows:

[0089] (1) Prepare an earthworm protease hydrolysate:

[0090] Under the environment with a frequency of 180 w, ultrasonically treat fresh earthworms for 40 min to obtain ultrasonically treated earthworms; then freeze the ultrasonically treated earthworms in an environment of -30°C for 60 min to obtain frozen earthworms, and pulverize the frozen earthworms to obtain pulverized earthworms;

[0091] Mix the pulverized earthworms, water, and a composite enzyme (the composite enzyme is obtained by mixing phytase, ligninase, and hemicellulase in a mass ratio of 0.2:1:1.2) in a mass ratio of 1:1.5:0.4 for enzymatic hydrolysis. The enzymatic hydrolysis time is 70 min, the enzymatic hydrolysis temperature is 52°C, and stirring is accompanied during the enzymatic hydrolysis process. The stirring speed is 150 rpm, and then an earthworm protease hydrolysate is obtained;

[0092] (2) By weight, the earthworm protein fertilizer includes: 10 parts of the earthworm protease hydrolysate in step (1), 23 parts of betel nut shell powder, 18 parts of rice straw, 8 parts of potassium sulfate, 12 parts of magnesium sulfate, and 15 parts of urea; the rice straw is the pulverized rice straw;

[0093] (3) According to the parts by mass in step (2), mix areca nut shell powder, rice straw, and earthworm protease hydrolysate, and then add a composite strain (the composite strain is composed of arbuscular mycorrhizal fungi, Klebsiella, and Bacillus mucilaginosus in a mass ratio of 1:2:2, and the effective viable count of arbuscular mycorrhizal fungi, Klebsiella, and Bacillus mucilaginosus is 0.5×10 7 cfu / mL), and perform composting treatment at 60°C for 10 days to obtain a fermented product; among them, the total mass of areca nut shell powder, rice straw, and earthworm protease hydrolysate and the volume ratio of the composite strain is 10 g:0.5 mL;

[0094] Mix the above fermented product, potassium sulfate, magnesium sulfate, and urea evenly to obtain earthworm protein fertilizer.

[0095] Control 7

[0096] A method for preparing earthworm protein fertilizer, the steps are as follows:

[0097] (1) Prepare earthworm protease hydrolysate:

[0098] Under the environment with a frequency of 180 w, ultrasonically treat fresh earthworms for 40 min to obtain ultrasonically treated earthworms; then freeze the ultrasonically treated earthworms in an environment of -30°C for 60 min to obtain frozen earthworms, and crush the frozen earthworms to obtain crushed earthworms;

[0099] Mix the crushed earthworms, water, and a composite enzyme (the composite enzyme is obtained by mixing phytase, ligninase, and hemicellulase in a mass ratio of 0.2:1:1.2) in a mass ratio of 1:1.5:0.4 for enzymatic hydrolysis. The enzymatic hydrolysis time is 70 min, the enzymatic hydrolysis temperature is 52°C, and stirring is accompanied during the enzymatic hydrolysis process. The stirring speed is 150 rpm, thereby obtaining earthworm protease hydrolysate;

[0100] (2) By weight, the earthworm protein fertilizer includes: 10 parts of the earthworm protease hydrolysate in step (1), 23 parts of areca nut shell powder, 18 parts of rice straw, 8 parts of potassium chloride, 12 parts of calcium magnesium phosphate fertilizer, and 15 parts of urea; among them, the rice straw is crushed rice straw;

[0101] (3) According to the parts by mass in step (2), mix areca nut shell powder, rice straw, and earthworm protease hydrolysate, and then add a composite strain (the composite strain is composed of arbuscular mycorrhizal fungi, Klebsiella, and Bacillus subtilis in a mass ratio of 1:2:2, and the effective viable count of arbuscular mycorrhizal fungi, Klebsiella, and Bacillus subtilis is 0.5×10 7 cfu / mL), and perform composting treatment at 60°C for 10 days to obtain a fermented product; among them, the total mass of areca nut shell powder, rice straw, and earthworm protease hydrolysate and the volume ratio of the composite strain is 10 g:0.5 mL;

[0102] Mix the above fermented product, potassium chloride, calcium magnesium phosphate fertilizer and urea evenly to obtain earthworm protein fertilizer.

[0103] Comparative Example 8

[0104] A preparation method of earthworm protein fertilizer, the steps are as follows:

[0105] (1) Prepare earthworm protease hydrolysate:

[0106] Under the environment with a frequency of 180w, ultrasonically treat fresh earthworms for 40 minutes to obtain ultrasonically treated earthworms; then freeze the ultrasonically treated earthworms in an environment of -30°C for 60 minutes to obtain frozen earthworms, and crush the frozen earthworms to obtain crushed earthworms;

[0107] Mix the crushed earthworms, water and complex enzyme (the complex enzyme is obtained by mixing phytase, ligninase and hemicellulase according to the mass ratio of 0.2:1:1.2) according to the mass ratio of 1:1.5:0.4 for enzymatic hydrolysis. The enzymatic hydrolysis time is 70 minutes, the enzymatic hydrolysis temperature is 52°C, and stirring is accompanied during the enzymatic hydrolysis process. The stirring speed is 150 rpm, and then earthworm protease hydrolysate is obtained;

[0108] (2) By weight, the earthworm protein fertilizer includes: 10 parts of the earthworm protease hydrolysate in step (1), 23 parts of betel nut shell powder, 18 parts of rice straw, 8 parts of potassium chloride, 12 parts of calcium magnesium phosphate fertilizer and 15 parts of urea; among them, the rice straw is crushed rice straw;

[0109] (3) According to the mass fraction in step (2), mix the betel nut shell powder, rice straw and earthworm protease hydrolysate and then add Bacillus mucilaginosus, and the effective viable count of Bacillus mucilaginosus is 0.5×10 7 cfu / mL), and perform composting treatment at 60°C for 10 days to obtain a fermented product; among them, the total mass of the betel nut shell powder, rice straw and earthworm protease hydrolysate and the volume ratio of Bacillus mucilaginosus is 10g:0.5mL;

[0110] Mix the above fermented product, potassium chloride, calcium magnesium phosphate fertilizer and urea evenly to obtain earthworm protein fertilizer.

[0111] Comparative Example 9

[0112] A preparation method of earthworm protein fertilizer, the steps are as follows:

[0113] (1) Prepare earthworm protease hydrolysate:

[0114] Under the environment with a frequency of 180 w, fresh earthworms were ultrasonically treated for 40 min to obtain ultrasonically treated earthworms; then the ultrasonically treated earthworms were frozen in an environment of -30 °C for 60 min to obtain frozen earthworms, and the frozen earthworms were crushed to obtain crushed earthworms;

[0115] The crushed earthworms, water and complex enzyme (the complex enzyme is obtained by mixing phytase, ligninase and hemicellulase according to a mass ratio of 0.2:1:1.2) were mixed for enzymatic hydrolysis according to a mass ratio of 1:1.5:0.4. The enzymatic hydrolysis time was 70 min, the enzymatic hydrolysis temperature was 52 °C, and stirring was carried out during the enzymatic hydrolysis process. The stirring speed was 150 rpm, and thus earthworm protease hydrolysate was obtained;

[0116] (2) Calculated by weight, the earthworm protein fertilizer includes: 10 parts of the earthworm protease hydrolysate in step (1), 23 parts of betel nut shell powder, 18 parts of rice straw, 8 parts of potassium chloride, 12 parts of calcium magnesium phosphate fertilizer and 15 parts of urea; among them, the rice straw is crushed rice straw;

[0117] (3) According to the mass parts in step (2), the betel nut shell powder, rice straw and earthworm protease hydrolysate were mixed and then compound bacteria were added (the compound bacteria are composed of arbuscular mycorrhizal fungi, Klebsiella and Bacillus mucilaginosus according to a mass ratio of 1:2:3, and the effective viable count of arbuscular mycorrhizal fungi, Klebsiella and Bacillus mucilaginosus is 0.5×10 7 cfu / mL), and composting treatment was carried out at 60 °C for 10 d to obtain a fermented product; among them, the total mass of the betel nut shell powder, rice straw and earthworm protease hydrolysate and the volume ratio of the compound bacteria is 10 g:1 mL;

[0118] The above fermented product, potassium chloride, calcium magnesium phosphate fertilizer and urea were mixed evenly to obtain the earthworm protein fertilizer.

[0119] Application Example 1

[0120] Taking the earthworm protein fertilizers prepared in Examples 1 and 2 and Comparative Examples 1-9 as the experimental groups, and at the same time using commercially available organic fertilizer and no fertilization as the control treatments, 10 betel nuts were selected for field fertilization for each treatment. Specifically:

[0121] Betel nuts with an average yield per single plant of 3.5 kg to 4 kg and basically the same growth vigor before the experiment were selected. Fertilization was carried out once in March and November of the year when the betel nuts were harvested. When fertilizing, ring fertilization was carried out about 35 cm away from the betel nut tree roots. Each betel nut was fertilized with 0.5 kg, and conventional field management was carried out; the average yield per single betel nut was counted in the following year, and the results are shown in Table 1.

[0122] Table 1 Comparison of yields of each fertilization treatment

[0123] Treatment Average yield per areca palm (kg) Example 1 12.54 Example 2 12.18 Comparative Example 1 10.17 Comparative Example 2 11.12 Comparative Example 3 9.74 Comparative Example 4 8.77 Comparative Example 5 10.15 Comparative Example 6 8.45 Comparative Example 7 9.17 Comparative Example 8 8.13 Comparative Example 9 7.48 No fertilization 3.79 Application of commercially available organic fertilizer 7.68

[0124] Combined with the data in Table 1, it can be seen that compared with the treatments of not applying fertilizer and applying commercially available organic fertilizer, the earthworm protein fertilizer provided by the present invention is more conducive to increasing the yield per plant of areca nut, with the highest reaching 12.54 kg / plant; while applying the earthworm protein fertilizer in the comparative example, the highest yield per plant is 11.12 kg / plant. It can be seen that the earthworm protein fertilizer prepared by adopting the technical scheme provided by the present invention has higher nutritional value and is more suitable for increasing the yield of areca nut.

[0125] In summary, it can be known that the fertilizer prepared by the present invention by compounding earthworm protease hydrolysate, areca nut shell powder, rice straw, potassium chloride, calcium magnesium phosphate fertilizer and urea has the characteristics of high nutritional value and good application effect. Applying it to areca nut can significantly increase the yield of areca nut.

[0126] Although the above embodiments have made a detailed description of the present invention, they are only a part of the embodiments of the present invention, rather than all embodiments. People can also obtain other embodiments without creative efforts based on this embodiment, and these embodiments all belong to the protection scope of the present invention.

Claims

1. An earthworm protein fertilizer, characterized in that: The earthworm protein fertilizer comprises: earthworm protein hydrolysate, betel nut shell powder, rice straw, potassium chloride, calcium magnesium phosphate fertilizer and urea; The preparation method of the earthworm protein hydrolysate comprises: The fresh earthworms are sequentially subjected to ultrasonic treatment, freezing and crushing to obtain crushed earthworms; The crushed earthworms, water and compound enzyme are mixed and hydrolyzed to obtain earthworm protein hydrolysate; The complex enzyme comprises: phytase, ligninase and hemicellulase.

2. The earthworm protein fertilizer according to claim 1, characterized in that: In parts by weight, the earthworm protein fertilizer comprises: 10-15 parts of earthworm protein hydrolysate, 23-28 parts of betel nut shell powder, 18-20 parts of rice straw, 5-8 parts of potassium chloride, 7-12 parts of calcium magnesium phosphate fertilizer and 10-15 parts of urea.

3. The earthworm protein fertilizer according to claim 1, characterized in that: The mass ratio of the phytase, the ligninase and the hemicellulase is 0.2-0.5:1:1.2-1.

4.

4. The earthworm protein fertilizer according to claim 1, characterized in that: The mass ratio of the crushed earthworms, water and complex enzyme is 1:1-1.5:0.4-0.

6.

5. The earthworm protein fertilizer according to claim 1, characterized in that: The time of the mixed enzymolysis is 70 to 85 minutes; the temperature of the mixed enzymolysis is 50 to 55°C.

6. The earthworm protein fertilizer according to claim 1, characterized in that: The frequency of the ultrasound is 150-180w; the time of the ultrasound is 35-45min; The freezing temperature is -25 to -35°C; the freezing time is 50 to 60 minutes.

7. The method for preparing the earthworm protein fertilizer according to any one of claims 1 to 6, characterized in that: The steps include: betel nut shell powder, rice straw, earthworm protein hydrolysate and composite bacteria are mixed and then composted to obtain a fermentation product; The fermented product, potassium chloride, calcium magnesium phosphate fertilizer and urea are uniformly mixed to obtain earthworm protein fertilizer; The composite bacterial species include: arbuscular mycorrhizal fungi, Klebsiella and jelly-like bacillus.

8. The preparation method according to claim 7, characterized in that: The effective viable counts of the arbuscular mycorrhizal fungi, Klebsiella and Bacillus jelly are ≥ 0.5×10 7 cfu / mL; The volume ratio of the arbuscular mycorrhizal fungi, Klebsiella and Bacillus gelatinosa is 1:2 to 2.5:

2.

9. The preparation method according to claim 7, characterized in that: During composting, the total mass ratio of the added volume of the composite bacteria to the total mass of the betel nut shell powder, rice straw and earthworm protein hydrolysate is 0.5-0.8 mL:10 g; The composting treatment time is 8-12 days; the composting treatment temperature is 45-60°C.

10. Use of the earthworm protein fertilizer according to any one of claims 1 to 6 in increasing betel nut yield.