Cell culture device and method

Abstract

The invention discloses a cell culture device and method, and relates to the technical field of cell culture equipment.The cell culture device comprises a culture box, a cell inlet, a culture solution inlet, a cell outlet, an observation window, a water tank, a control panel, a constant-temperature control box, a water spraying assembly, a driving motor, a rotating mechanism used for driving a cleaning assembly to conduct circumferential cleaning and the cleaning assembly; the cleaning assembly comprises a connecting column, a cleaning disc, bristles and a cleaning pad. The cleaning disc is of a circular disc-shaped structure and is fixed below the connecting column, a plurality of cylindrical grooves are uniformly formed in the bottom of the cleaning disc in the circumferential direction, and the cylindrical grooves are used for placing cleaning pads; the bristles are fixed at the bottom of the cleaning disc and used for directly contacting and assisting in cleaning the inner wall of the incubator; the multiple cleaning pads are fixed in the corresponding cylindrical grooves correspondingly, and negative pressure cavities are formed between the cleaning pads and the cylindrical grooves. The technical effects that the adaptability is enhanced, the cleaning strength is uniform, the cleaning effect is improved, healthy growth and reproduction of cells are facilitated, and the cell culture success rate is improved can be achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture equipment, and in particular to a cell culture device and method. Background Art

[0002] Cell culture enables cells to grow and proliferate normally in vitro by providing the nutrients, growth factors and suitable environmental conditions required for cell growth. However, due to the poor tolerance of cells to various external factors, there may be many chemical substances and microbial contaminations that have toxic effects on cells, thereby affecting cell growth and experimental results. Therefore, it is crucial to provide cells with a clean growth environment.

[0003] For cell culture, a Chinese invention patent with patent number CN113046239B discloses a cell culture device, including a culture box, the upper end surface of the culture box is detachably connected to a box cover, the upper end surface of the box cover is installed with a motor, the lower end surface of the box cover is fixedly connected to a first bearing, and multiple nozzles spray water from the water tank to spray and rinse the inside of the culture box, and the cleaning effect is greatly improved with the bristles. When the motor is started, the cleaning disk is driven to rotate, thereby driving two scrapers to rotate, and the suspended cells coated on the inner wall of the culture box are scraped off, further improving the cleaning effect, so that the inside of the culture box is kept clean, which is conducive to the next cell culture work; this patent solves the problem that the existing cell culture device is usually cleaned manually after use, the cleaning effect is poor and time-consuming and labor-intensive, and if the suspended cells attached to the inner wall of the culture device cannot be thoroughly cleaned, it will affect the next cell sheet culture effect, which is not conducive to cell culture work.

[0004] Although the above scheme improves the culture efficiency to a certain extent during the cell culture process, in actual use, the simple combination of the cleaning disc and the bristles is difficult to adapt to the tiny bumps and unevenness of the inner wall of the incubator, resulting in the bristles being unable to fit tightly to the inner wall surface during the cleaning process, thus affecting the cleaning effect; in addition, the hard cleaning disc and bristle combination may not be able to evenly distribute the cleaning force during the cleaning process, which can easily cause uneven cleaning force, which may cause damage to the inner wall, incomplete cleaning of some areas, and low cleaning efficiency. These problems not only affect the service life of the incubator, but may also increase the risk of contamination during the cell culture process, which is not conducive to the healthy growth and reproduction of cells, and reduces the success rate of cell culture and the accuracy of experimental results. Summary of the invention

[0005] The present application solves the technical problems in the prior art of insufficient adaptability, uneven cleaning force, poor cleaning effect, being unfavorable for the healthy growth and reproduction of cells, and reduced cell culture success rate by providing a cell culture device and method, and achieves the technical effects of enhanced adaptability, uniform cleaning force, improved cleaning effect, being conducive to the healthy growth and reproduction of cells, and increased cell culture success rate.

[0006] The present application provides a cell culture device, including a culture box, a cell inlet, a culture fluid inlet, a cell outlet, an observation window, a water tank, a control panel, a constant temperature control box, a water spray assembly, a driving motor, a rotating mechanism for driving a cleaning assembly to perform circumferential cleaning, and a cleaning assembly; The cleaning assembly is used to clean the inner wall of the incubator, and includes a connecting column, a cleaning plate, bristles and a cleaning pad; The connecting column is fixed under the rotating mechanism, and is used to connect the cleaning disc and the rotating mechanism to ensure that the cleaning disc rotates with the rotating mechanism; the cleaning disc is a circular disc-shaped structure, which is fixed under the connecting column and has a plurality of cylindrical grooves evenly opened on its bottom along its circumference, and the cylindrical grooves are used to place the cleaning pad; the bristles are fixed at the bottom of the cleaning disc, and are used to directly contact and assist in cleaning the inner wall of the incubator; a plurality of cleaning pads are provided, which correspond one to one with the cylindrical grooves, and are respectively fixed in the corresponding cylindrical grooves to form a negative pressure cavity between the cleaning pads and the cylindrical grooves.

[0007] Furthermore, the bristles are made of PET, which has good wear resistance and elasticity, and the length of the bristles is 2 to 5 mm.

[0008] Furthermore, the cleaning pad is an airbag structure having two layers, namely a negative pressure layer and a cleaning layer; The negative pressure layer is fixed inside the cylindrical groove and interacts with the negative pressure cavity; the cleaning layer is fixed inside the cylindrical groove and is located below the negative pressure layer; a gap is left between the negative pressure layer and the cleaning layer, and the interior of the gap is connected to the air pump through a pipeline. The expansion degree of the cleaning pad is controlled by controlling the internal inflation volume, thereby adjusting the fit between the cleaning pad and the inner wall of the incubator and the cleaning strength.

[0009] Furthermore, the volume of the cleaning pad fully expanded under the action of inflation is 2 to 4 times the volume in the initial state.

[0010] Furthermore, the cleaning layer is a capsule structure having two layers, namely, an isolation layer and a liquid permeation layer; The isolation layer is fixed inside the cylindrical groove and below the negative pressure layer, and is used to isolate the gas from the liquid seepage layer; the liquid seepage layer is fixed inside the cylindrical groove and below the isolation layer, and is in direct contact with the inner wall of the incubator for cleaning.

[0011] Furthermore, a gap is left between the isolation layer and the liquid permeable layer, the interior of which is connected to a liquid pump via an external pipeline, and a cleaning liquid is introduced into the interior for chemically cleaning the stains on the inner wall of the incubator with the cleaning liquid.

[0012] Furthermore, the permeable layer is a permeable surface structure and has a plurality of micropores uniformly provided on its surface. The micropores are used to seep cleaning liquid to the inner wall of the incubator. By controlling the internal inflation amount of the cleaning pad during the cleaning process, the squeezing force of the gas on the isolation layer is controlled, thereby controlling the rate at which the permeable layer seeps cleaning liquid, thereby adjusting the degree of fit with the inner wall of the incubator and achieving pressure cleaning of the inner wall by the cleaning liquid.

[0013] Furthermore, the micropores are of a conical structure, and the pore diameter gradually decreases from the inside to the outside, so as to ensure that the cleaning liquid forms a higher speed and pressure when spraying.

[0014] Furthermore, a plurality of protrusions are evenly fixed on the outside of the liquid-permeable layer and along its curved surface, the protrusions being annular in structure, each of the protrusions having a microhole in the middle, and each of the four protrusions in a square array also having a microhole in the middle. The four protrusions constitute a large cleaning surface and cooperate with the microholes for pressure cleaning, a single protrusion constitutes a small cleaning surface and wraps the indentation through a single protrusion to form a tiny enclosed space, and cooperates with the microholes for pressure cleaning.

[0015] In addition, the technical solution of the present invention also provides a cell culture method, the specific steps are as follows: Step 1: First, the user connects the cell sample to be cultured into the incubator through the cell inlet for culture and injects the cell culture medium into the incubator through the culture medium inlet; Step 2: Observe the working conditions inside the incubator in real time through the observation window, and adjust the temperature suitable for cell culture in real time through the control panel to control the constant temperature control box according to the suitable temperature conditions for cell culture. After the cell culture is completed, discharge the cell culture fluid through the cell outlet, and take out the cultured cell tissue; Step 3: Start the drive motor and drive the rotating mechanism to start working. The rotating mechanism drives the cleaning disc to move to the inner wall of the incubator and performs circumferential rotation cleaning under the drive of the drive motor. The bristles and the cleaning pad begin to contact and clean the inner wall of the incubator. Step 4: During the cleaning process, a negative pressure cavity is formed between the cleaning pad and the cylindrical groove. Under the action of negative pressure, the cleaning pad better adapts to and fits the shape, slight changes and unevenness of the inner wall of the incubator. The negative pressure suction effect enhances the fit of the cleaning pad and the bristles to the inner wall of the incubator. Step 5: The water spray component is started to spray the water in the water tank to rinse the inner wall of the incubator. After the cleaning is completed, the drive motor stops working, the cleaning disk stops rotating, and the cleaning component returns to its initial position driven by the rotating mechanism, so that the inside of the incubator remains clean for the next cell culture work.

[0016] One or more technical solutions provided in this application have at least the following technical effects or advantages: By using a soft silicone material for the cleaning pad, tiny scratches or damage to the inner wall of the incubator can be avoided during the cleaning process; and a negative pressure chamber is provided so that the cleaning pad can better adapt to the tiny bumps and unevenness of the inner wall of the incubator under negative pressure, so that the bristles can fit more closely to the inner wall surface, so that the bristles can better maintain their shape and cleaning strength during the cleaning process, effectively solving the technical problems of insufficient adaptability, uneven cleaning strength, poor cleaning effect, being unfavorable to the healthy growth and reproduction of cells, and reduced cell culture success rate in the prior art, and achieving the technical effects of enhanced adaptability, uniform cleaning strength, improved cleaning effect, being favorable to the healthy growth and reproduction of cells, and increased cell culture success rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a diagram of the overall internal structure of a cell culture device of the present invention.

[0018] Figure 2 This is a back-facing overall external structure diagram of a cell culture device of the present invention.

[0019] Figure 3 This is a schematic diagram of the state of a cleaning component of a cell culture device of the present invention when cleaning the inner wall of a culture box.

[0020] Figure 4 The figure is a schematic diagram of the three-dimensional structure of a cleaning component of a cell culture device of the present invention.

[0021] Figure 5 The present invention is a schematic diagram of the three-dimensional structure of a cleaning plate, a cleaning pad assembly and bristles of a cell culture device.

[0022] Figure 6 The present invention is a three-dimensional cross-sectional view of a cleaning plate and a cleaning pad assembly of a cell culture device.

[0023] Figure 7 The present invention is a partial three-dimensional cross-sectional view of the cleaning disk and cleaning layer of a cell culture device.

[0024] Figure 8 It is a partial cross-sectional view of a negative pressure layer and a cleaning layer of a cell culture device of the present invention.

[0025] Fig. 9The figure is a schematic diagram of the three-dimensional structure of the liquid permeation layer of a cell culture device of the present invention.

[0026] Fig.10 The present invention is a full cross-sectional view of a negative pressure layer, an isolation layer and a liquid permeation layer of a cell culture device.

[0027] Fig.11 It is a schematic diagram of a group of protrusions and corresponding micro-holes of a cell culture device of the present invention when pressure cleaning is performed in the enclosed area thereof.

[0028] Fig.12 It is a schematic diagram of the state of a cell culture device of the present invention when the cleaning liquid seeps out from the micropores under the action of air pressure and penetrates into the inner wall indentations for pressure cleaning.

[0029] In the figure: 100, incubator; 101, cell inlet; 102, culture medium inlet; 103, cell outlet; 104, observation window; 110, water tank; 120, control panel; 130, constant temperature control box; 140, water spray assembly; 150, drive motor; 200, rotating mechanism; 300, cleaning assembly; 310, connecting column; 320, cleaning plate; 321, cylindrical groove; 330, bristles; 340, cleaning pad; 341, negative pressure layer; 350, cleaning layer; 351, isolation layer; 352, permeable layer; 353, micropores; 360, bumps; 370, cleaning solution. DETAILED DESCRIPTION

[0030] To facilitate the understanding of the present invention, the present application will be described more comprehensively below with reference to the relevant drawings; the drawings show preferred embodiments of the present invention, but the present invention can be implemented in many different forms and is not limited to the embodiments described herein; on the contrary, the purpose of providing these embodiments is to enable a more thorough and comprehensive understanding of the disclosed content of the present invention.

[0031] It should be noted that the terms “vertical”, “horizontal”, “up”, “down”, “left”, “right” and similar expressions used in this document are only for illustrative purposes and do not represent the only implementation method.

[0032] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by technicians in the technical field to which the present invention belongs; the terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention; the term "and / or" used herein includes any and all combinations of one or more related listed items.

[0033] See also Figure 1, which is a schematic diagram of the overall structure of a cell culture device of the present invention; the cell culture device of the present application uses a soft silicone material for the cleaning pad 340, so as to avoid causing tiny scratches or damage to the inner wall of the incubator 100 during the cleaning process; and a negative pressure chamber is provided, so that the cleaning pad 340 can better adapt to the tiny bumps and unevenness of the inner wall of the incubator 100 under negative pressure, so that the bristles 330 can fit the inner wall surface more closely, so that the bristles 330 can better maintain their shape and cleaning force during the cleaning process; the technical effects of enhanced adaptability, uniform cleaning force, improved cleaning effect, conducive to the healthy growth and reproduction of cells and improved cell culture success rate are achieved.

[0034] Embodiment 1: Figures 1 to 6 As shown, the present application is a cell culture device, including a culture box 100, a cell inlet 101, a culture solution inlet 102, a cell outlet 103, an observation window 104, a water tank 110, a control panel 120, a constant temperature control box 130, a water spray assembly 140, a driving motor 150, a rotating mechanism 200 for driving a cleaning assembly 300 to perform circumferential cleaning, and a cleaning assembly 300; The cleaning assembly 300 is used to clean the inner wall of the incubator 100, and includes a connecting column 310, a cleaning plate 320, bristles 330 and a cleaning pad 340; The connecting column 310 is fixed below the rotating mechanism 200 and is used to connect the cleaning disc 320 and the rotating mechanism 200 to ensure that the cleaning disc 320 rotates with the rotating mechanism 200; The cleaning plate 320 is a circular plate-shaped structure, fixed below the connecting column 310 and having a plurality of cylindrical grooves 321 evenly formed at the bottom along the circumference thereof, wherein the cylindrical grooves 321 are used to place the cleaning pad 340; The bristles 330 are fixed to the bottom of the cleaning plate 320 and are used to directly contact and assist in cleaning the inner wall of the incubator 100; The cleaning pads 340 are provided in a plurality and correspond to the cylindrical grooves 321 one by one. The cleaning pads 340 are respectively fixed in the corresponding cylindrical grooves 321 and form negative pressure chambers between the cleaning pads 340 and the cylindrical grooves 321 .

[0035] The bristles 330 are made of PET, which has good wear resistance and elasticity. The length of the bristles 330 is 2 to 5 mm.

[0036] The rotating mechanism 200 is used to drive the cleaning disc 320 to move to the inner wall position of the incubator 100 and clean the inner wall under the drive of the driving motor 150, and includes a first bearing, a first rotating shaft, an upper transmission disc, an upper transmission shaft, an upper connecting rod, a hinge wheel, a lower connecting rod, a second bearing, a second rotating shaft, a lower transmission disc, a lower transmission shaft, etc. The position of the cleaning component 300 is switched by the cooperation of the upper transmission shaft and the lower transmission shaft and the rotation of the hinge wheel; the water spray component 140 is used to spray and rinse the inside of the incubator 100, and is equipped with a nozzle for spraying water on the incubator 100; all of them are prior arts and will not be described in detail here.

[0037] The method for using the cell culture device (cell culture method) of the embodiment of the present application is: Step 1: First, the user connects the cell sample to be cultured into the incubator 100 through the cell inlet 101 for culture and injects the cell culture medium into the incubator 100 through the culture medium inlet 102; Step 2: Observe the working conditions inside the incubator 100 in real time through the observation window 104, and control the constant temperature control box 130 to adjust the temperature suitable for cell culture in real time through the control panel 120 according to the suitable temperature conditions for cell culture. After the cell culture is completed, discharge the cell culture fluid through the cell outlet 103, and take out the cultured cell tissue; Step 3: Start the driving motor 150 and drive the rotating mechanism 200 to start working. The rotating mechanism 200 drives the cleaning disc 320 to move to the inner wall of the incubator 100 and performs circumferential rotation cleaning under the drive of the driving motor 150. The bristles 330 and the cleaning pad 340 begin to contact and clean the inner wall of the incubator 100. Step 4: During the cleaning process, since a negative pressure cavity is formed between the cleaning pad 340 and the cylindrical groove 321, under the action of negative pressure, the cleaning pad 340 better adapts to and fits the shape, slight changes and unevenness of the inner wall of the incubator 100, and the negative pressure suction effect enhances the fit of the cleaning pad 340 and the bristles 330 to the inner wall of the incubator 100; Step 5: The water spray assembly 140 is started to spray the water in the water tank 110 to spray and rinse the inner wall of the incubator 100. After the cleaning is completed, the drive motor 150 stops working, the cleaning disk 320 stops rotating, and the cleaning assembly 300 returns to the initial position driven by the rotating mechanism 200, so that the inside of the incubator 100 remains clean for the next cell culture work.

[0038] The technical solutions in the above embodiments of the present application have at least the following technical effects or advantages: In the present application, the cleaning pad 340 is made of soft silicone material and a negative pressure chamber is provided, so that the cleaning pad 340 can better adapt to the tiny bumps and unevenness of the inner wall of the incubator 100 under negative pressure, so that the bristles 330 can fit more closely to the inner wall surface, thereby enabling the bristles 330 to better maintain their shape and cleaning force during the cleaning process. At the same time, the cleaning pad 340 made of soft material can avoid causing tiny scratches or damage to the inner wall of the incubator 100 during the cleaning process, and can better absorb and disperse the impact force during the cleaning process, thereby distributing the cleaning force more evenly, improving the cleaning effect, and enhancing the thoroughness of cleaning. It is not only beneficial to maintain the integrity of the inner wall of the incubator 100 and extend its service life, but also beneficial to reduce the risk of contamination during cell culture. The cleaning pad 340 with tight fit and good wear resistance further reduces the possibility of cell damage and contamination caused by cleaning tools. The cleaned interior of the incubator 100 provides cells with a more stable and sterile growth environment, which is beneficial to the healthy growth and reproduction of cells, reduces the failure rate of cell culture caused by improper cleaning, and improves the success rate and efficiency of cell culture. It solves the technical problems of insufficient adaptability, uneven cleaning force, poor cleaning effect, unfavorable for the healthy growth and reproduction of cells, and reduced cell culture success rate in the prior art, and achieves the technical effects of enhanced adaptability, uniform cleaning force, improved cleaning effect, favorable for the healthy growth and reproduction of cells, and improved cell culture success rate.

[0039] Embodiment 2: In order to further control the cleaning force of the bristles 330 on the inner wall of the incubator 100 and the degree of fit between the cleaning pad 340, the bristles 330 and the inner wall cleaning surface, the present application proposes the following technical solutions for the above technical problems, specifically: like Figure 6 and Figure 7 As shown, the cleaning pad 340 is an airbag structure, having two layers, namely a negative pressure layer 341 and a cleaning layer 350; The negative pressure layer 341 is fixed inside the cylindrical groove 321 and interacts with the negative pressure chamber; the cleaning layer 350 is fixed inside the cylindrical groove 321 and is located below the negative pressure layer 341; a gap is left between the negative pressure layer 341 and the cleaning layer 350, and the interior of the negative pressure layer 341 is connected to the air pump through a pipeline. The expansion degree of the cleaning pad 340 is controlled by controlling the internal inflation volume, thereby adjusting the fit and cleaning strength of the cleaning pad 340 and the inner wall of the incubator 100.

[0040] The volume of the cleaning pad 340 fully expanded under the effect of inflation is 2 to 4 times the volume in the initial state.

[0041] The present application optimizes and improves the structure of the cleaning pad 340. The airbag structure can be used to adjust the expansion degree of the cleaning pad 340 by precisely controlling the amount of gas filled, thereby achieving fine control of the cleaning force of the bristles 330 on the inner wall of the incubator 100. The adjustability makes the cleaning process more adaptable to the degree of stains and material properties of the inner wall of the incubator 100, so that the device can flexibly adjust the cleaning force when the degree of pollution of the inner wall is different or different parts need to be cleaned, flexibly adapt to and meet different cleaning needs, and achieve the best cleaning effect. At the same time, the airbag structure of the cleaning pad 340 can be adaptively adjusted according to the shape and tiny bumps of the inner wall of the incubator 100, so that the bristles 330 fit more closely to the inner wall. The optimization of the fit helps to improve the cleaning efficiency and ensure that every corner can be fully cleaned. The tight fit reduces the residue of cleaning liquid 370 and stains on the inner wall of the incubator 100, further reduces the risk of contamination during cell culture, and ensures the stability of the cell culture process.

[0042] Embodiment 3: Considering that the inner wall of the incubator 100 may produce tiny dents or cracks due to wear or accidental collision, and in the process of cell culture, different types of cells will produce different metabolites during metabolism, such as acidic substances, alkaline substances, proteins, nucleic acids, etc. These metabolites may react chemically with substances on the surface of the incubator 100 to form dirt that is difficult to clean. If the cell metabolites exist in the dents or cracks on the inner wall of the incubator 100, it will make the incubator 100 more difficult to clean and easily become a breeding ground for microorganisms such as bacteria, fungi, mycoplasma, etc., increasing the risk of contamination of cell culture. In view of the above technical problems, this application proposes the following technical solutions, specifically: like Figures 8 to 9 As shown, the cleaning layer 350 is a capsule structure, having two layers, namely, an isolation layer 351 and a liquid permeable layer 352; The isolation layer 351 is fixed inside the cylindrical groove 321 and located below the negative pressure layer 341, and is used to isolate the gas from the permeable layer 352; the permeable layer 352 is fixed inside the cylindrical groove 321 and located below the isolation layer 351, and is in direct contact with the inner wall of the incubator 100 for cleaning.

[0043] A gap is left between the isolation layer 351 and the liquid permeable layer 352 , the interior of which is connected to a liquid pump via an external pipe, and a cleaning liquid 370 is introduced into the interior for chemically cleaning stains on the inner wall of the incubator 100 with the cleaning liquid 370 .

[0044] The permeable layer 352 is a permeable surface structure and has a plurality of micropores 353 evenly formed on its surface. The micropores 353 are used to seep the cleaning liquid 370 to the inner wall of the incubator 100. The inflation volume inside the cleaning pad 340 is controlled during the cleaning process, and the squeezing force of the gas on the isolation layer 351 is controlled, thereby controlling the rate at which the cleaning liquid 370 seeps out of the permeable layer 352. While adjusting the degree of fit with the inner wall of the incubator 100, pressure cleaning of the inner wall by the cleaning liquid 370 is achieved.

[0045] The present application can flexibly adjust the rigidity of the outer layer (i.e., the liquid permeable layer 352) of the cleaning pad 340 by controlling the amount of liquid 370 filled in. This adjustability makes the cleaning process more adaptable to the hardness and stain characteristics of the inner wall of different incubators 100, thereby improving the comprehensiveness and efficiency of cleaning; the micropores 353 structure can be provided to allow the cleaning liquid 370 to seep out. When inflated, the micropores 353 expand to facilitate the discharge of the cleaning liquid 370, so that the cleaning liquid 370 can be more evenly distributed on the inner wall of the incubator 100, thereby improving the thoroughness of cleaning; Fig.12 As shown, since the outer layer of the cleaning pad 340 (i.e., the permeable layer 352) is made of a soft material, it can be tightly attached to the position of the dent on the inner wall during the cleaning process to form a closed space. In the closed space, the cleaning liquid 370 slowly seeps out from the micropores 353 and penetrates into the inside of the dent for thorough cleaning. The pressure spraying of the cleaning liquid 370 can impact the stains in the dent and react with it quickly chemically, so as to more fully contact and react with the stains, more effectively remove the stains and microorganisms in the dent, reduce cleaning dead corners, and help improve the penetration effect and cleaning efficiency of the cleaning liquid 370; it also reduces the residue of the cleaning liquid 370 and stains on the inner wall of the incubator 100, further reduces the risk of contamination during the cell culture process, and provides a strong guarantee for the healthy growth of cells.

[0046] Embodiment 4: In order to further improve the effect of pressure cleaning and increase the success rate of cell culture, the present application proposes the following technical solutions for the above technical problems, specifically: like Figures 10 to 12 As shown, the micropores 353 are of a conical structure, and the aperture gradually decreases from the inside to the outside, so as to ensure that the cleaning liquid 370 forms a higher speed and pressure when spraying.

[0047] A plurality of protrusions 360 are evenly fixed on the outside of the liquid-permeable layer 352 and along its curved surface. The protrusions 360 are annular structures. A microhole 353 is opened in the middle of each of the protrusions 360. A microhole 353 is also opened in the middle of every four protrusions 360 in a square array. The four protrusions 360 constitute a large cleaning surface and cooperate with the microholes 353 to perform pressure cleaning. A single protrusion 360 constitutes a small cleaning surface and wraps the dent with a single protrusion 360 to form a tiny enclosed space, and cooperates with the microholes 353 to perform pressure cleaning.

[0048] When the embodiment of the present application is actually running, the steps are as follows: Step 1: First, the user connects the cell sample to be cultured into the incubator 100 through the cell inlet 101 for culture and injects the cell culture medium into the incubator 100 through the culture medium inlet 102, observes the internal working conditions of the incubator 100 in real time through the observation window 104, and controls the constant temperature control box 130 to adjust the temperature suitable for cell culture in real time through the control panel 120 according to the suitable temperature conditions for cell culture; Step 2: After the cell culture is completed, the cell culture fluid is discharged through the cell outlet 103, and the cultured cell tissue is taken out; Step 3: According to the degree of stains and material properties of the inner wall of the incubator 100, the amount of gas filled into the cleaning pad 340 is adjusted to control its expansion degree and cleaning strength; at the same time, the amount of cleaning liquid 370 filled into the liquid permeable layer 352 is adjusted to ensure that the cleaning liquid 370 can evenly seep out from the micropores 353 during the expansion process; Step 4: Start the driving motor 150 and the rotating mechanism 200 to start working. The rotating mechanism 200 drives the cleaning disc 320 to rotate, and the bristles 330 and the cleaning pad 340 begin to contact and clean the inner wall of the incubator 100. The negative pressure system starts to work, and a negative pressure cavity is formed between the negative pressure layer 341 and the cylindrical groove 321, so as to enhance the fit between the cleaning pad 340 and the inner wall of the incubator 100. When the cleaning pad 340 expands and fits the inner wall, the micropores 353 on the liquid permeable layer 352 expand under the action of air pressure, and the cleaning liquid 370 is ejected from the conical micropores 353 at a high speed, forming a higher impact force. The protrusions 360 wrap the dents to form a tiny enclosed space. The cleaning liquid 370 forms high pressure in the enclosed space, and penetrates deeper into the dents on the inner wall of the incubator 100 for cleaning. Step 5: The water spray assembly 140 is started to spray the water in the water tank 110 to spray and rinse the inner wall of the incubator 100, and the bristles 330 and the cleaning pad 340 are used to improve the cleaning effect. After the cleaning is completed, the drive motor 150 stops working, the cleaning disk 320 stops rotating, and the cleaning assembly 300 returns to the initial position, so that the inside of the incubator 100 remains clean, which is conducive to the next cell culture work.

[0049] The present application sets the micropores 353 to a conical structure, and the diameter gradually decreases from the inside to the outside, so that the aperture of the cleaning liquid 370 becomes smaller when it is sprayed out. Under the same pressure, the cleaning liquid 370 is sprayed out at a greater speed, which can form a stronger impact force and can more effectively impact the stains in the dent, which helps to quickly break down the stains and make them easier to be dissolved and taken away by the cleaning liquid 370, and also helps to improve the comprehensiveness of cleaning and reduce cleaning dead corners; the dent can be wrapped at multiple points by the protrusions 360 to form a tiny enclosed space. In the tiny enclosed space, the cleaning liquid 370 sprayed from the micropores 353 can form a higher pressure, which helps the cleaning liquid 370 to penetrate deeper into the dent, fully contact and take effect on the stains.

[0050] like Fig.11 As shown, four adjacent protrusions 360 can form a large cleaning surface, which, in conjunction with the micropores 353 therebetween, can cover a wider area, while a single protrusion 360 forms a small cleaning surface, which can accurately clean tiny indentations. Through the cooperation of the protrusions 360 and the micropores 353, stains and microorganisms in the indentations can be more thoroughly removed. This cleaning method further reduces the residue of cleaning liquid 370 and stains on the inner wall of the incubator 100, reduces the risk of contamination during cell culture, and provides more favorable environmental conditions for the healthy growth and reproduction of cells.

[0051] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A cell culture device, comprising a culture box (100), a cell inlet (101), a culture fluid inlet (102), a cell outlet (103), an observation window (104), a water tank (110), a control panel (120), a constant temperature control box (130), a water spray assembly (140), a drive motor (150), a rotating mechanism (200) for driving a cleaning assembly (300) to perform circumferential cleaning, and a cleaning assembly (300), characterized in that: The cleaning assembly (300) is used to clean the inner wall of the incubator (100), and comprises a connecting column (310), a cleaning plate (320), bristles (330) and a cleaning pad (340); The connecting column (310) is fixed below the rotating mechanism (200) and is used to connect the cleaning disc (320) and the rotating mechanism (200) to ensure that the cleaning disc (320) rotates with the rotating mechanism (200); The cleaning disc (320) is a circular disc-shaped structure, fixed below the connecting column (310) and having a plurality of cylindrical grooves (321) uniformly formed at its bottom along its circumference, the cylindrical grooves (321) being used to place a cleaning pad (340); the bristles (330) are fixed at the bottom of the cleaning disc (320) and are used to directly contact and assist in cleaning the inner wall of the incubator (100); a plurality of cleaning pads (340) are provided, corresponding one to one with the cylindrical grooves (321), and are respectively fixed in corresponding cylindrical grooves (321) and form a negative pressure cavity between the cleaning pads (340) and the cylindrical grooves (321).

2. A cell culture device according to claim 1, characterized in that: The bristles (330) are made of PET, which has good wear resistance and elasticity, and the length of the bristles (330) is 2 to 5 mm.

3. A cell culture device according to claim 1, characterized in that: The cleaning pad (340) is an airbag structure having two layers, namely a negative pressure layer (341) and a cleaning layer (350); The negative pressure layer (341) is fixed inside the cylindrical groove (321) and interacts with the negative pressure chamber; the cleaning layer (350) is fixed inside the cylindrical groove (321) and is located below the negative pressure layer (341); a gap is left between the negative pressure layer (341) and the cleaning layer (350), and the inside of the negative pressure layer (341) is connected to an air pump through a pipeline, and the expansion degree of the cleaning pad (340) is controlled by controlling the internal inflation volume, thereby adjusting the fit between the cleaning pad (340) and the inner wall of the incubator (100) and the cleaning strength.

4. A cell culture device according to claim 3, characterized in that: The volume of the cleaning pad (340) fully expanded under the action of inflation is 2 to 4 times the volume in the initial state.

5. A cell culture device as claimed in claim 3, characterized in that: The cleaning layer (350) is a capsule structure having two layers, namely an isolation layer (351) and a liquid permeation layer (352); The isolation layer (351) is fixed inside the cylindrical groove (321) and located below the negative pressure layer (341), and is used to isolate the gas from the liquid permeation layer (352); the liquid permeation layer (352) is fixed inside the cylindrical groove (321) and located below the isolation layer (351), and is in direct contact with the inner wall of the incubator (100) for cleaning.

6. A cell culture device according to claim 5, characterized in that: A gap is left between the isolation layer (351) and the liquid permeable layer (352), the interior of which is connected to a liquid pump via an external pipeline, and a cleaning liquid (370) is introduced into the interior, so as to chemically clean stains on the inner wall of the incubator (100) using the cleaning liquid (370).

7. A cell culture device according to claim 6, characterized in that: The liquid permeable layer (352) is a permeable surface structure and has a plurality of micropores (353) uniformly formed on its surface. The micropores (353) are used to permeate the cleaning liquid (370) to the inner wall of the incubator (100). By controlling the amount of air in the cleaning pad (340) during the cleaning process, the squeezing force of the gas on the isolation layer (351) is controlled, thereby controlling the rate at which the cleaning liquid (370) permeates the liquid permeable layer (352). While adjusting the degree of contact with the inner wall of the incubator (100), pressure cleaning of the inner wall by the cleaning liquid (370) is achieved.

8. A cell culture device according to claim 7, characterized in that: The micropores (353) are of a conical structure, with the pore diameter gradually decreasing from the inside to the outside, so as to ensure that the cleaning liquid (370) forms a higher speed and pressure when being sprayed out.

9. A cell culture device according to claim 8, characterized in that: A plurality of protrusions (360) are evenly fixed on the outside of the liquid permeable layer (352) and along its curved surface. The protrusions (360) are annular in structure. A microhole (353) is provided in the middle of each protrusion (360). A microhole (353) is also provided in the middle of every four protrusions (360) in a square array. The four protrusions (360) form a large cleaning surface and cooperate with the microholes (353) to perform pressure cleaning. A single protrusion (360) forms a small cleaning surface and wraps the indentation through the single protrusion (360) to form a tiny enclosed space, and cooperates with the microholes (353) to perform pressure cleaning.

10. A cell culture method, characterized in that: The cell culture device according to claim 1 is used, and the specific steps are as follows: Step 1: First, the user connects the cell sample to be cultured into the incubator (100) through the cell inlet (101) for culture and injects the cell culture medium into the incubator (100) through the culture medium inlet (102); Step 2: Observe the working conditions inside the incubator (100) in real time through the observation window (104), and according to the suitable temperature conditions for cell culture, control the constant temperature control box (130) through the control panel (120) to adjust the temperature suitable for cell culture in real time. After the cell culture is completed, discharge the cell culture fluid through the cell outlet (103), and take out the cultured cell tissue; Step 3: Start the driving motor (150) and drive the rotating mechanism (200) to start working, the rotating mechanism (200) drives the cleaning plate (320) to move to the inner wall of the incubator (100) and performs circumferential rotation cleaning under the drive of the driving motor (150), and the bristles (330) and the cleaning pad (340) begin to contact and clean the inner wall of the incubator (100); Step 4: During the cleaning process, since a negative pressure cavity is formed between the cleaning pad (340) and the cylindrical groove (321), under the action of negative pressure, the cleaning pad (340) better adapts to and fits the shape, slight changes and unevenness of the inner wall of the incubator (100), and the negative pressure suction effect enhances the fit of the cleaning pad (340) and the bristles (330) to the inner wall of the incubator (100); Step 5: The water spray assembly (140) is started to spray the water in the water tank (110) to rinse the inner wall of the culture box (100). After the cleaning is completed, the drive motor (150) stops working, the cleaning disk (320) stops rotating, and the cleaning assembly (300) returns to the initial position under the drive of the rotating mechanism (200), so that the inside of the culture box (100) remains clean, ready for the next cell culture work.

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