Parallel control method, system and equipment for multiple experimental equipment for PCR (Polymerase Chain Reaction)

Abstract

A multi-experimental equipment parallel control method for PCR (polymerase chain reaction) comprises the following steps: providing an intelligent parallel control end, acquiring experimental scheme data, and checking all experimental resources of a stack; performing classification on each PCR reaction according to the requirements of a PCR program; each classified PCR reaction is distributed to each experimental device, and all experimental resources required by the scheme are obtained at the same time; when it is detected that the experiment scheme is correct and the experiment resources are sufficient, all the experiment resources are distributed to the experiment equipment according to the priorities of the experiment resources; before a control instruction of experimental resource allocation is sent, experimental resource occupation conditions of all devices are obtained, if the resources are occupied, the instruction is temporarily skipped, and experimental resources of a second priority are allocated to the devices; the experiment resource occupation condition of each device is detected in real time, and the instruction sending sequence is changed in real time.

Description

Technical Field

[0001] The present invention relates to the field of computers, and particularly to a method, a system, and a device for parallel control of multiple experimental devices for PCR. Background Art

[0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. The substances participating in the PCR reaction mainly include five types, namely: primers (PCR primers are DNA fragments, and the primers for DNA replication in cells are a segment of RNA strand), enzymes, dNTPs, templates, and buffers.

[0003] When there are more PCR reactions, the transfer and mixing actions of reagents will be more frequent and complex, and there will often be a situation where the experimental devices cannot be parallel due to the occupation of reagents. Considering the efficiency of PCR reactions and the effective duration of reagents, etc., the original experimental device control system can no longer well meet the experimental requirements. Summary of the Invention

[0004] One of the purposes of the present invention is to provide a method, a system, and a device for parallel control of multiple experimental devices for PCR, which can cope with the situation of more PCR reactions and complex transfer and mixing actions of reagents, and perform parallel control on multiple experimental devices to improve efficiency.

[0005] One of the purposes of the present invention is to provide a method, a system, and a device for parallel control of multiple experimental devices for PCR, which can communicate with each experimental device simultaneously by changing the original control instruction logic, so as to avoid possible primer reagent conflicts when sending control instructions.

[0006] One of the purposes of the present invention is to provide a method, a system, and a device for parallel control of multiple experimental devices for PCR, which can perform intelligent parallel control on multiple experimental devices, and the occupation and release of reagents as resources are faster, and at the same time, the whole set of control instructions is made more intuitive and efficient.

[0007] One of the purposes of the present invention is to provide a method, a system, and a device for parallel control of multiple experimental devices for PCR, which can automatically generate control instructions according to the experimental scheme and the resources in the stack, and flexibly execute according to the priorities of each reaction in the scheme during the process.

[0008] One of the purposes of the present invention is to provide a method, a system, and a device for parallel control of multiple experimental devices for PCR, which can reduce labor costs.

[0009] One of the objectives of the present invention is to provide a method, a system, and a device for parallel control of multiple experimental devices for PCR, which can dynamically record the status of each instruction, the status of each experimental device, and the reagent resources occupied by the device in a database or the device itself during the execution of the entire set of control instructions.

[0010] One of the objectives of the present invention is to provide a method, a system, and a device for parallel control of multiple experimental devices for PCR. Users only need to generate a plan in the system according to experimental requirements, and the entire process from the start to the end of the plan is managed by the control system, making it more convenient to use.

[0011] One of the objectives of the present invention is to provide a method, a system, and a device for parallel control of multiple experimental devices for PCR. Experimental resources are allocated to each experimental device according to the priority order of the plan, and then each experimental device synchronously executes its respective control instructions. After use, the resources are quickly released so that the resources can be used by other devices more quickly later, improving efficiency.

[0012] One of the objectives of the present invention is to provide a method, a system, and a device for parallel control of multiple experimental devices for PCR. At the beginning of the process, the entire set of control instructions is generated, and at the same time, by communicating with the experimental devices, the occupancy of experimental resources of each device is understood, and the sending order of the instructions is changed in real time to ensure that no experimental device will be idle due to lack of available resources.

[0013] To achieve at least one of the invention objectives of the present invention, the present invention provides a method for parallel control of multiple experimental devices for PCR, including the following steps:

[0014] Provide an intelligent parallel control terminal, obtain experimental plan data, and check all experimental resources in the stack;

[0015] Classify each PCR reaction according to the requirements of the PCR program;

[0016] Allocate the classified PCR reactions to each experimental device, and at the same time obtain all experimental resources required by the plan;

[0017] When it is detected that the experimental plan is correct and the experimental resources are sufficient, allocate each experimental resource to the experimental device according to its priority;

[0018] Before sending the control instruction for experimental resource allocation, obtain the occupancy of experimental resources of all devices. If the resources are already occupied, temporarily skip this instruction and allocate the experimental resources of the second priority to this device; and

[0019] Real-time detect the occupancy of experimental resources of each device and change the sending order of the instructions in real time.

[0020] In some embodiments, the method for parallel control of multiple experimental devices for PCR further includes the step of: when it is detected that the stack experimental resources are insufficient to complete the entire scheme, prompt for information supplementation through the human-machine interaction terminal.

[0021] In some embodiments, the method for parallel control of multiple experimental devices for PCR further includes the step of: when the experimental device receives resources, it will record and feedback that the resources have been occupied by it, and work step by step according to the control instructions received by it.

[0022] In some embodiments, the method for parallel control of multiple experimental devices for PCR further includes the step of: generating a complete set of control instructions at the beginning of the process.

[0023] In some embodiments, the method for parallel control of multiple experimental devices for PCR further includes the step of: during the execution of the complete set of control instructions, dynamically record the status of each instruction, the status of each experimental device, and the reagent resources occupied by the experimental device.

[0024] In some embodiments, the method for parallel control of multiple experimental devices for PCR further includes the step of: detecting and distinguishing the scenarios of PCR experimental resource occupancy.

[0025] In some embodiments, the PCR program parameters are selected from: temperature, temperature gradient, time, number of cycles, temperature merging, temperature gradient merging, and time merging.

[0026] According to another aspect of the present invention, there is also provided a computer-readable storage medium, on which a computer program is stored, and when the computer program is executed by a processor, it executes the steps of the method for parallel control of multiple experimental devices for PCR.

[0027] According to another aspect of the present invention, there is also provided a device for parallel control of multiple experimental devices for PCR, including:

[0028] A memory for storing software application programs,

[0029] A processor for executing the software application programs, and each program of the software application programs correspondingly executes each step of the method for parallel control of multiple experimental devices for PCR.

[0030] According to another aspect of the present invention, there is also provided a parallel control system for multiple experimental devices used in PCR, which applies the parallel control method for multiple experimental devices used in PCR. The parallel control system for multiple experimental devices used in PCR includes an intelligent parallel control unit, which provides an intelligent parallel control terminal and is configured to execute: automatically generate control instructions according to the experimental protocol and the resources in the stack, and execute according to the priorities of each reaction in the protocol during the process; during the execution of the entire set of control instructions, the status of each instruction, the status of each experimental device, and the reagent resources occupied by the experimental device will be dynamically recorded in the database or in the local machine of each experimental device; the experimental resources are allocated to each experimental device according to the priority order of the protocol, and then each experimental device synchronously executes its own control instructions, and quickly releases the experimental resources after use, so that the experimental resources can be used by other experimental devices in time subsequently. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 FIG. is a flowchart of the steps of a parallel control method for multiple experimental devices used in PCR according to an embodiment of the present invention.

[0032] Figures 2 - 4 FIG. is a comparison chart of the pipetting time for PCR.

[0033] Figure 5 FIG. is an example diagram of a PCR program. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0034] The following description is used to disclose the present invention so that those skilled in the art can implement the present invention. The preferred embodiments described below are only examples, and other obvious variations can be thought of by those skilled in the art. The basic principles defined in the following description can be applied to other implementation schemes, variant schemes, improvement schemes, equivalent schemes, and other technical schemes that do not deviate from the spirit and scope of the present invention.

[0035] It can be understood that the term "a" should be understood as "at least one" or "one or more". That is, in one embodiment, the number of an element can be one, while in other embodiments, the number of the element can be multiple. The term "a" cannot be understood as a limitation on the number.

[0036] The present invention is an invention related to computer programs. As Figure 1The figure shows a flowchart of a parallel control method for multiple experimental devices used in PCR based on the present invention, which elaborates on a solution for controlling or processing external or internal objects of a computer by executing a computer program compiled according to the above process based on the computer program processing flow to solve the problems proposed by the present invention. Through the parallel control method for multiple experimental devices used in PCR of the present invention, a computer system can be utilized to cope with the situation of a large number of PCR reactions and complex reagent transfer and mixing operations, and perform intelligent parallel control on multiple experimental devices to improve efficiency. Through the parallel control method for multiple experimental devices used in PCR of the present invention, it is possible to avoid potential primer reagent conflicts during the sending of control instructions by changing the original control instruction logic and communicating with each experimental device simultaneously; it can perform intelligent parallel control on multiple experimental devices, enabling faster occupation and release of reagents as resources, and making the entire set of control instructions more intuitive and efficient; it can automatically generate control instructions based on the experimental protocol and the resources in the stack, and flexibly execute them according to the priorities of each reaction in the protocol during the process; it can reduce labor costs; during the execution of the entire set of control instructions, the status of each instruction, the status of each experimental device, and the reagent resources occupied by the device will be dynamically recorded in the database or the device itself; the user only needs to generate a protocol in the system according to the experimental requirements, and the entire process from the start to the end of the protocol is managed by the control system, making it more convenient to use; the experimental resources are allocated to each experimental device according to the priority order of the protocol, and then each experimental device synchronously executes its own control instructions, and quickly releases the resources after use, enabling the resources to be used by other devices more quickly later, improving efficiency; at the beginning of the process, the entire set of control instructions is generated, and at the same time, by communicating with the experimental devices, the occupation situation of the experimental resources of each device is understood, and the sending order of the instructions is changed in real time to ensure that no experimental device will be idle due to lack of available resources. It can be understood that the "computer" referred to in the present invention not only refers to devices such as desktop computers, laptops, and tablets, but also includes other intelligent electronic devices that can run according to programs and process data.

[0037] Specifically, the parallel control method for multiple experimental devices used in PCR includes the following steps:

[0038] S100: Provide an intelligent parallel control terminal, obtain experimental protocol data, and check all experimental resources in the stack;

[0039] S101: Classify each PCR reaction according to the requirements of the PCR program;

[0040] S102: Allocate the classified PCR reactions to each experimental device, and at the same time obtain all experimental resources required by the protocol;

[0041] S103: Feedback that the control plan for this experiment has been generated and recorded in the database;

[0042] S104: Feedback the status that each experimental device has not yet started running;

[0043] S105: When it is detected that the stack experimental resources are insufficient to complete the entire plan, prompt the staff to make supplements through the human-machine interaction terminal;

[0044] S106: When it is detected that the experimental plan is correct and the experimental resources are sufficient, allocate each experimental resource to the experimental device according to its priority;

[0045] S107: When the experimental device receives the resources, record and feedback that the resources have been occupied by it, and work step by step according to the control instructions it receives;

[0046] S108: Immediately release the experimental resources after they are used up, clear the occupancy records of relevant materials, and then execute the next control;

[0047] S109: Before sending the control instruction for experimental resource allocation, obtain the experimental resource occupancy status of all devices. If the resource has been occupied, temporarily skip this instruction and allocate the experimental resources of the second priority to this device;

[0048] Among them, in the step S101, the PCR program parameters include: temperature, temperature gradient, time, number of cycles, temperature merging, temperature gradient merging, time merging.

[0049] Furthermore, the parallel control method for multiple experimental devices used for PCR further includes the following steps to solve the resource occupancy situation:

[0050] S201: Classify according to the execution of the PCR program;

[0051] S202: Allocate the classified reactions to each pipetting device;

[0052] S203: The pipetting device requests experimental resources such as primer plates, transfer plates, reaction plates, etc. according to the reaction. When the required primer plate is not occupied, the device will directly obtain this resource and occupy it until it is used up; if it is occupied, the device will directly apply for the primer plate of the second priority;

[0053] S204: When the device obtains the primer plate, transfer plate / reaction plate, it can start to execute the plan. After the primer is used up, it will be directly released;

[0054] S205: After the primer plate is released, if the plan has not ended, continue to cycle and apply for the primer plates required in the plan; if the plan ends, move the reaction plate to the PCR instrument.

[0055] Further, the parallel control method for multiple experimental devices for PCR further includes the following steps for parallel control of PCR experimental resource occupancy:

[0056] Detect and distinguish the scenarios of PCR experimental resource occupancy, and the scenario definitions are divided into completely non - overlapping, partially overlapping, and completely overlapping; among them, each scenario is defined and divided into two cases: MIX and repair.

[0057] Obtain the scenario information of PCR experimental resource occupancy, and calculate the duration comparison for each scenario.

[0058] Among them, in a specific embodiment, through the duration calculation and comparison for each scenario, it can be seen that:

[0059] Scenario 1. Completely non - overlapping, MIX: The time consumption is very close.

[0060] Scenario 2. Completely non - overlapping, repair: The time consumption is very close.

[0061] Scenario 3. Partially overlapping, MIX: The probability of this situation occurring is very high. When there is one overlap, the control system can speed up by 7%.

[0062] Scenario 4. Partially overlapping, repair: The probability of this situation occurring is very high. The original system has a large time consumption, and the improvement is very obvious.

[0063] Scenario 5. Completely overlapping, MIX: The original system has a large time consumption, and the improvement is very obvious.

[0064] Scenario 6. Completely overlapping, repair: The original system has a large time consumption, and the improvement is very obvious.

[0065] Through the parallel control method for multiple experimental devices for PCR of the present invention, it is possible to ensure the reasonable allocation of experimental resources when multiple experimental devices are controlled in parallel, and ensure that there will be no situation where the device is idle due to resource occupancy, making the entire work process more reasonable and improving the working efficiency of the device.

[0066] Those skilled in the art can understand that the embodiments of the present invention can be provided in the form of a method, a system, or a computer program product. Therefore, the present invention can take the form of an all - hardware embodiment, an all - software embodiment, or an embodiment combining software and hardware.

[0067] The present invention can be embedded in a computer program product, which includes all the features enabling the implementation of the methods described herein. The computer program product is contained in one or more computer-readable storage media, which have computer-readable program code contained therein. According to another aspect of the present invention, there is also provided a computer-readable storage medium, on which a computer program is stored. When the computer program is executed by a processor, it can execute the steps of the method for parallel control of multiple experimental devices for PCR according to the present invention. A computer storage medium is a medium in a computer memory for storing a certain discontinuous physical quantity. Computer storage media include, but are not limited to, semiconductors, disk memories, magnetic cores, magnetic drums, magnetic tapes, laser discs, etc. Those skilled in the art can understand that computer storage media are not limited to the foregoing examples, and the foregoing examples are only for illustration and are not limited to the present invention.

[0068] According to another aspect of the present invention, there is also provided a device for parallel control of multiple experimental devices for PCR. The device for parallel control of multiple experimental devices for PCR includes: a software application program, a memory for storing the software application program, and a processor for executing the software application program. Each program of the software application program can correspondingly execute the steps in the method for parallel control of multiple experimental devices for PCR according to the present invention.

[0069] A typical combination of hardware and software can be a general computer system with a computer program. When the program is loaded and executed, it controls the computer system, so that the method for parallel control of multiple experimental devices for PCR disclosed in the present invention can be executed.

[0070] Those skilled in the art can understand that the device can be embodied as a desktop computer, a notebook, a mobile intelligent device, etc., but the foregoing are only for illustration and also include other intelligent analysis devices equipped with the software application program of the present invention.

[0071] Those skilled in the art can understand that the method for parallel control of multiple experimental devices for PCR according to the present invention can be implemented by hardware, software, or a combination of software and hardware. The present invention can be implemented in a centralized manner in at least one computer system, or be implemented in a decentralized manner by different parts distributed in several interconnected computer systems. Any computer system or other device that can implement the method is applicable. A typical combination of common software and hardware can be a general computer system installed with a computer program. By installing and executing the program, the computer system is controlled to operate according to the method.

[0072] Corresponding to the embodiments of the method of the present invention, according to another aspect of the present invention, there is also provided a parallel control system for multiple experimental devices for PCR. The parallel control system for multiple experimental devices for PCR is an application of the parallel control method for multiple experimental devices for PCR of the present invention in computer program improvement. Through the parallel control system for multiple experimental devices for PCR of the present invention, a computer system can be utilized to handle the situation of a large number of PCR reactions and complex reagent transfer and mixing operations, and perform intelligent parallel control on multiple experimental devices to improve efficiency. Through the parallel control method for multiple experimental devices for PCR of the present invention, it is possible to change the original control instruction logic and communicate with each experimental device simultaneously, so as to avoid possible primer reagent conflicts when sending control instructions; it is possible to perform intelligent parallel control on multiple experimental devices, and the occupation and release of reagents as resources are faster, while making the whole set of control instructions more intuitive and efficient; it can automatically generate control instructions according to the experimental protocol and the resources in the stack, and flexibly execute according to the priorities of each reaction in the protocol during the process; it can reduce labor costs; during the execution process of the whole set of control instructions, the status of each instruction, the status of each experimental device, and the reagent resources occupied by the device will be dynamically recorded in the database or the device itself; the user only needs to generate a protocol in the system according to the experimental requirements, and the whole process from the start to the end of the protocol is managed by the control system, which is more convenient to use; the experimental resources are allocated to each experimental device according to the priority order of the protocol, and then each experimental device synchronously executes its own control instructions, and the resources are quickly released after use, so that the resources can be used by other devices faster subsequently, improving the efficiency; the whole set of control instructions are generated at the beginning of the process, and at the same time, by communicating with the experimental devices, the occupation of experimental resources by each device is understood, and the sending order of the instructions is changed in real time to ensure that no experimental device will be idle due to lack of available resources.

[0073] Specifically, the parallel control system for multiple experimental devices for PCR includes an intelligent parallel control unit. The intelligent parallel control unit provides an intelligent parallel control terminal and is configured to execute: automatically generate control instructions according to the experimental protocol and the resources in the stack, and execute according to the priorities of each reaction in the protocol during the process; during the execution process of the whole set of control instructions, the status of each instruction, the status of each experimental device, and the reagent resources occupied by the device will be dynamically recorded in the database or each experimental device itself; the experimental resources are allocated to each experimental device according to the priority order of the protocol, and then each experimental device synchronously executes its own control instructions, and the resources are quickly released after use, so that the resources can be used by other devices faster subsequently.

[0074] More specifically, the intelligent parallel control unit includes an experimental resource inspection module, a PCR reaction classification module, a PCR reaction allocation module, and a control scheme module.

[0075] When the experimental resource inspection module obtains an experimental scheme, it inspects all the experimental resources in the stack; the PCR reaction classification module classifies each PCR reaction according to the requirements of the PCR program. Among them, the PCR program parameters include: temperature, temperature gradient, time, number of cycles, temperature merging, temperature gradient merging, and time merging; the PCR reaction allocation module obtains the classification information of the PCR reaction classification module and allocates it to each experimental device accordingly. At the same time, according to the experimental device and the reaction scheme it is allocated, all the experimental resources required by the experimental device can be determined; at this time, the control scheme module feedbacks that the control scheme of the experiment has been generated in the control system, but at this time, each experimental device has not yet run, and it is recorded in the database.

[0076] The intelligent parallel control unit further includes a stack experimental resource shortage feedback module. After the control scheme is generated, when it is detected that the stack experimental resources are insufficient to complete the entire scheme, the stack experimental resource shortage feedback module gives a feedback prompt, so that the staff can supplement information through the human-computer interaction module.

[0077] The intelligent parallel control unit further includes a scheme resource detection module and an experimental resource priority allocation module. When the scheme resource detection module detects that the experimental scheme is correct and the experimental resources are sufficient, the experimental resource priority allocation module is configured to execute: allocate each experimental resource to the corresponding experimental device according to its priority. After each experimental device receives the resource, it will record that the resource has been occupied by it, and work step by step according to the control instructions it receives. After the experimental resource is used up, it will be released immediately, and the occupation record of the relevant data will be cleared, and then the next control will be executed.

[0078] The parallel control system for multiple experimental devices for PCR further includes an experimental resource occupation situation control unit, which is configured to execute: before sending the control instruction for experimental resource allocation, obtain the experimental resource occupation situation of all devices. If the experimental resource has been occupied, the instruction will be temporarily skipped, and the experimental resource of the second priority will be allocated to the experimental device.

[0079] More specifically, the experimental resource occupation situation control unit includes a PCR experimental resource occupation situation scenario definition module, which is configured to execute: define the PCR experimental resource occupation situation as three scenarios: completely non-crossed, partially crossed, and completely crossed; each scenario is further divided into two situations: MIX and repair.

[0080] The experimental resource occupancy control unit further includes a scene duration calculation module, which is used to obtain the scene information of the PCR experimental resource occupancy situation scene definition module and calculate the duration of the corresponding scene.

[0081] In a specific embodiment, the scene duration comparison results of the scene duration calculation module are as follows:

[0082] Scene 1. Completely non - overlapping, MIX: The time consumption is very close.

[0083] Scene 2. Completely non - overlapping, repair: The time consumption is very close.

[0084] Scene 3. Partially overlapping, MIX: The probability of this situation occurring is very high. When there is one overlap, the control system can speed up by 7%.

[0085] Scene 4. Partially overlapping, repair: The probability of this situation occurring is very high. The original system has a large time consumption, and the improvement is very obvious.

[0086] Scene 5. Completely overlapping, MIX: The original system has a large time consumption, and the improvement is very obvious.

[0087] Scene 6. Completely overlapping, repair: The original system has a large time consumption, and the improvement is very obvious.

[0088] It is worth mentioning that through the parallel control system for multiple experimental devices for PCR of the present invention, it can ensure the reasonable allocation of experimental resources when multiple experimental devices are controlled in parallel, and ensure that there will be no situation where the device is idle due to resource occupancy, making the entire work process more reasonable and improving the working efficiency of the device.

[0089] Those skilled in the art can understand that the present invention has been illustrated with reference to the flowcharts and / or block diagrams of the method, system, and computer program product according to the present invention. Each block in the flowchart and / or block diagram, and the combination of blocks in the flowchart and / or block diagram can obviously be implemented by computer program instructions. These computer program instructions can be provided to the processor of a general - purpose computer, a special - purpose computer, an embedded processor, or other programmable data - processing devices to generate a machine, so that the instructions (the instructions through the processor of the computer or other programmable data - processing devices) generate a device for realizing the functions specified in one or more blocks of the flowchart and / or block diagram.

[0090] Those skilled in the art should understand that the embodiments of the present invention described above and shown in the drawings are only examples and do not limit the present invention. The object of the present invention has been fully and effectively achieved. The function and structural principle of the present invention have been shown and described in the embodiments. Without departing from this principle, the embodiments of the present invention can have any deformation or modification.

Claims

1. A method for parallel control of multiple experimental equipment for PCR, characterized in that: The method for parallel control of multiple experimental devices for PCR comprises the following steps: Provide intelligent parallel control terminal to obtain experimental plan data and check all experimental resources in the stack; Classify each PCR reaction according to the requirements of the PCR program; Allocate the classified PCR reactions to various experimental equipment and obtain all the experimental resources required for the plan; When it is detected that the experimental plan is correct and the experimental resources are sufficient, the various experimental resources are allocated to the experimental equipment according to their priority; Before sending the control instruction for experimental resource allocation, obtain the experimental resource occupancy status of all devices. If the resource is already occupied, temporarily skip the instruction and allocate the experimental resource of the second priority to the device; and Detect the experimental resource usage of each device in real time and change the order of sending instructions in real time.

2. The method for parallel control of multiple experimental devices for PCR as described in claim 1, wherein the method for parallel control of multiple experimental devices for PCR further comprises the step of: when it is detected that the stack experimental resources are insufficient to complete the entire plan, prompting execution information supplement through the human-computer interaction terminal.

3. The method for parallel control of multiple experimental devices for PCR as described in claim 1, wherein the method for parallel control of multiple experimental devices for PCR further includes the following steps: when the experimental device receives a resource, it records and feeds back that the resource has been occupied by it, and works step by step according to the control instructions it receives.

4. The method for parallel control of multiple experimental devices for PCR as described in claim 1, wherein the method for parallel control of multiple experimental devices for PCR further comprises the step of: generating a complete set of control instructions at the beginning of the process.

5. The method for parallel control of multiple experimental equipment for PCR as described in claim 1, wherein the method for parallel control of multiple experimental equipment for PCR also includes the step of dynamically recording the status of each instruction, the status of each experimental equipment, and the reagent resources occupied by the experimental equipment during the execution of the entire set of control instructions.

6. The method for parallel control of multiple experimental devices for PCR as described in claim 1, wherein the method for parallel control of multiple experimental devices for PCR further comprises the step of detecting and distinguishing scenarios of PCR experimental resource occupancy.

7. The method for parallel control of multiple experimental devices for PCR as described in any one of claims 1 to 6, wherein the PCR program parameters are selected from: temperature, temperature gradient, time, number of cycles, temperature merging, temperature gradient merging and time merging.

8. A computer-readable storage medium having a computer program stored thereon, characterized in that: When the computer program is executed by a processor, the steps of the method for parallel control of multiple experimental devices for PCR described in any one of claims 1 to 7 are performed.

9. A parallel control device for multiple experimental devices for PCR, characterized in that: include: Memory, for storing software applications, A processor is used to execute the software application, and each program of the software application correspondingly executes each step of the parallel control method for multiple experimental equipment for PCR as described in any one of claims 1 to 7.

10. A parallel control system for multiple experimental equipment for PCR, using the parallel control method for multiple experimental equipment for PCR as claimed in any one of claims 1 to 7, characterized in that: The parallel control system for multiple experimental equipment used for PCR includes an intelligent parallel control unit, which provides an intelligent parallel control terminal and is configured to execute: automatically generate control instructions according to the experimental plan and the resources in the stack, and execute them according to the priority of each reaction in the plan; during the execution of the whole set of control instructions, the status of each instruction, the status of each experimental equipment and the reagent resources occupied by the experimental equipment will be dynamically recorded in the database or each experimental equipment; the experimental resources are allocated to each experimental equipment according to the priority order of the plan, and then each experimental equipment synchronously executes its own control instructions, and quickly releases the experimental resources after use, so that the experimental resources can be used by other experimental equipment in time later.

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