A testing device for animal experiments in brain science based on optogenetic technology

Abstract

The present invention belongs to the field of biological experimental technologies, and particularly relates to a testing device for animal experiments in brain science based on optogenetic technology, comprising a main body, on which a spectrometer is installed, and a cover is plugged on the main body, and replacement blocks are plugged on the covers; an odor component, which is arranged on the main body; a hilly component, which includes a rubber skin connected to the inner cavity of the main body; a maze component, which includes a partition board, a lifting plate is connected to the lower end of the inner cavity of the main body, and a replacement plate is connected to the position between adjacent partition boards at the lower end of the inner cavity of the main body. The invention can install modules with different functions on the device according to requirements, simulate road surfaces of different shapes, and can also simulate road surfaces such as hills or deep pits according to requirements to test different reactions of animals, and can change the path of the maze after the animal enters the maze, avoiding the generation of muscle memory due to the animal walking multiple times and affecting the accuracy of the experiment.

Description

Technical Field

[0001] The present invention belongs to the technical field of biological experiments, and particularly relates to a testing device for brain science animal experiments based on optogenetic technology. Background Art

[0002] Brain science animal experiments are the core means to analyze the relationship between neural circuit mechanisms and behaviors. Among them, optogenetic technology provides a revolutionary tool for causal research by precisely manipulating the activities of specific neurons. However, existing experimental devices have systematic defects in the accuracy of environmental simulation, the coordination of multimodal stimuli, and the analysis of behavioral responses, resulting in low experimental repeatability and single data dimension.

[0003] First of all, traditional behavioral testing devices rely on fixed spatial structures. Animals can form path dependence after 5 - 7 trainings, which cannot continuously stimulate the dynamic plasticity of the hippocampal - prefrontal circuit. Moreover, the replacement efficiency of physical obstacles is low, and the replacement amplitude is large, and dynamic threat scenarios such as the approach of predators cannot be simulated in real time. The overly rigid plane cannot simulate natural terrain, resulting in distorted research data on the somatosensory cortex - motor cortex coordination mechanism.

[0004] Secondly, animal behavior is dynamically regulated by multiple factors such as temperature, humidity, light, and smell. Different brain regions have significant differences in environmental sensitivity. Although existing devices can adjust these parameters, they are basically adjusted as a whole, and the release sources of parameters such as smell and light cannot be positionally regulated, and thus the test effects cannot be viewed diversely. Summary of the Invention

[0005] The purpose of the present invention is to provide a testing device for brain science animal experiments based on optogenetic technology, which can be equipped with modules with different functions according to requirements, simulate road surfaces of different shapes, and can also simulate road surfaces such as hills or deep pits according to requirements to test different reactions of animals, and can change the path of the maze after the animal enters the maze to avoid the formation of muscle memory due to repeated walking of the organism, affecting the accuracy of the experiment.

[0006] The technical solution adopted by the present invention is specifically as follows:

[0007] A testing device for brain science animal experiments based on optogenetic technology, comprising:

[0008] A main body, on the upper end of which a spectrometer is fixedly installed, and two covers are movably inserted on the upper end of the main body, and replacement blocks are movably inserted on both of the two covers;

[0009] An odor component, which is arranged on the upper end of the main body;

[0010] Hilly component, the hilly component includes six groups of rubber skins, and the six groups of rubber skins are fixedly connected to the lower end of the inner cavity of the main body;

[0011] Labyrinth component, the labyrinth component includes a partition board, a lifting plate is slidably connected to the position near the partition board at the lower end of the inner cavity of the main body, and a replacement plate is fixedly connected to the position between adjacent partition boards at the lower end of the inner cavity of the main body;

[0012] Among them, the replacement block is used to replace different functional modules, the rubber skin rises or falls along the inner cavity of the main body to simulate hills or deep pits, the lifting plate is used to change the path of the partition board, and the replacement plate is used to simulate different road surfaces.

[0013] In a preferred scheme, the lower end of the spectrometer penetrates through the main body and is fixedly connected with an optical fiber, the upper end of the main body is fixedly installed with a lamp control board, the lamp control board is electrically connected with an LED lamp, the LED lamp is movably inserted into the cover, and the lower end of the LED lamp extends into the inner cavity of the main body.

[0014] In a preferred scheme, two through grooves are opened at the upper end of each group of covers, and the LED lamp and the replacement block are both movably inserted into the through grooves. Magnet attracting plates are fixedly connected to both sides of the through grooves at the upper end of the cover, and the upper ends of the magnet attracting plates are in contact connection with the outsides of the replacement block and the LED lamp.

[0015] In a preferred scheme, the odor component includes an air pump, the air pump is fixedly connected to the upper end of the main body, the outlet of the air pump is communicated with a blowing head, the blowing head is movably inserted into the through groove at the upper end of the cover, and an air inlet box is fixedly connected to the upper end of the main body, and the air inlet box is communicated with the inlet of the air pump.

[0016] In a preferred scheme, the odor component further includes a placement rack, the placement rack is movably inserted into the upper end of the inner cavity of the air inlet box, a limiting tube is fixedly connected to the upper end of the inner cavity of the placement rack, a lifting rod is slidably connected to the inner cavity of the limiting tube, the lower end of the lifting rod extends out of the limiting tube and is fixedly connected with a pressing plate, the pressing plate is movably sleeved in the inner cavity of the placement rack, a lead screw is rotatably connected to the upper end of the placement rack, the upper end of the lead screw extends out of the placement rack, and the outer circle of the lower end of the lead screw is threadedly connected to the inner cavity of the lifting rod.

[0017] In a preferred scheme, the hilly component further includes a driving rod, the driving rod is fixedly connected to the lower end of the rubber skin, and the lower end of the driving rod is slidably connected to the lower end of the inner cavity of the main body. A spring is movably sleeved on the outer circle of the driving rod, the lower end of the spring is fixedly connected to the lower end of the inner cavity of the main body, a pull rope is fixedly connected to the part of the lower end of the driving rod extending into the inner cavity of the main body, the other end of the pull rope penetrates through the main body and extends out of the main body, and a winding wheel is rotatably connected to the lower end of the outer side of the main body, and the end of the pull rope extending out of the main body is fixedly connected to the inside of the winding wheel.

[0018] In a preferred embodiment, a convex plate is fixedly connected to the outer ring at the upper end of the driving rod, the upper end of the spring is fixedly connected to the lower end of the convex plate, the lower end of the spring is fixedly connected to the lower end of the inner cavity of the main body, and the driving rod is movably sleeved in the inner ring of the spring.

[0019] In a preferred embodiment, the labyrinth assembly further includes four air bags. The four air bags are fixedly connected to the bottom end of the inner cavity of the main body, the upper ends of the four air bags are respectively fixedly connected to the lower ends of the four lifting plates, the lower ends of the four air bags are all communicated with air pipes, the other ends of the four air pipes all extend out of the main body, and the parts of the four air pipes extending out of the main body are all communicated with valves. The inlets and outlets of the four valves are all communicated with shunt pipes, and the middle of the shunt pipe is communicated with an inlet and outlet pipe.

[0020] In a preferred embodiment, an empty groove is formed at the position between adjacent partitions at the lower end of the inner cavity of the main body, and an adsorption plate is fixedly connected in the empty groove. The replacement plate is in contact connection with the upper end of the adsorption plate.

[0021] In a preferred embodiment, both the adsorption plate and the magnetic attraction plate are magnetic attraction elements.

[0022] The technical effects achieved by the present invention are as follows:

[0023] The replacement block and the replacement plate of the present invention can be used to install modules with different functions on the device according to requirements and simulate road surfaces of different shapes, thereby improving the diversity of detection; before detection, the replacement block can be removed, and according to the requirements of detection, the replacement block can be replaced with modules with functions such as light, smell, air conditioning, etc. And before the optogenetic experiment detection, the replacement plate can be replaced with structures of different road surface shapes, such as wavy and frosted, further improving the diversity of detection, observing the situation of animals on different road surfaces, and thus obtaining more experimental data;

[0024] The hilly component of the present invention can change the situation of the road surface at the lower end of the inner cavity of the main body according to requirements, thereby simulating road surfaces such as hills or deep pits and testing different reactions of animals; during the optogenetic experiment detection, the driving part of the hilly component can be rotated according to requirements, pulling or releasing the rubber skin to make the rubber skin contract or rise, thereby simulating the state of a deep pit or a hill, and through the regulation of multiple groups of rubber skins, a more continuous and diverse ground surface can be simulated, and thus the reactions of animals under different ground surfaces can be detected, obtaining more experimental and detection data;

[0025] The maze component of the present invention can change the path of the maze according to requirements, avoiding the formation of muscle memory by the organism walking multiple times and affecting the accuracy of the experiment. When conducting the experiment, when the organism enters the maze, the inflation part of the maze component can be driven to inflate, lifting the lifting plate, thereby changing the maze path formed by the partition plates. And when needed, the height of the lifting plate can be changed to form a semi-blocking structure, thereby increasing the diversity of the experiment and avoiding affecting the accuracy of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 is the overall structural schematic diagram of the present invention;

[0027] Figure 2 is the structural schematic diagram of the main body of the present invention;

[0028] Figure 3 is the structural schematic diagram of the cover of the present invention;

[0029] Figure 4 is the bottom view schematic diagram of the LED lamp of the present invention;

[0030] Figure 5 is the cross-sectional schematic diagram of the air inlet box of the present invention;

[0031] Figure 6 is the disassembled cross-sectional schematic diagram of the odor component of the present invention;

[0032] Figure 7 is the position schematic diagram of the hilly component of the present invention;

[0033] Figure 8 is the cross-sectional schematic diagram of the hilly component of the present invention;

[0034] Figure 9 is the disassembled schematic diagram of the spring and the drive rod of the present invention;

[0035] Figure 10 is the position schematic diagram of the maze component of the present invention;

[0036] Figure 11 is the disassembled schematic diagram of the replacement plate and the adsorption plate of the present invention;

[0037] Figure 12 is the structural schematic diagram of the partition plate of the present invention;

[0038] Figure 13 is the cross-sectional schematic diagram of the maze component of the present invention.

[0039] In the drawings, the list of components represented by each reference numeral is as follows:

[0040] 10. Main body; 11. Spectrometer; 12. Optical fiber; 13. Cover; 14. Replacement block; 15. Lamp control board; 16. LED lamp; 17. Magnetic adsorption board; 20. Odor component; 21. Air pump; 22. Blowing head; 23. Air inlet box; 24. Placing rack; 25. Limiting tube; 26. Lifting rod; 27. Extrusion plate; 28. Lead screw; 30. Hill component; 31. Rubber skin; 32. Driving rod; 33. Spring; 34. Pulling rope; 35. Reel; 40. Maze component; 41. Partition board; 42. Lifting plate; 43. Airbag; 44. Air pipe; 45. Valve; 46. Shunt pipe; 47. Inlet and outlet pipe; 48. Adsorption plate; 49. Replacement plate. Detailed implementation mode

[0041] In order to make the above objects, features and advantages of the present invention more obvious and understandable, the following will make a detailed description of the specific implementation mode of the present invention with reference to the accompanying drawings of the specification.

[0042] In the following description, many specific details are set forth in order to fully understand the present invention. However, the present invention can also be implemented in other ways different from those described herein. Those skilled in the art can make similar generalizations without departing from the connotation of the present invention. Therefore, the present invention is not limited by the specific embodiments disclosed below.

[0043] Secondly, the so-called "one embodiment" or "embodiment" herein refers to a specific feature, structure or characteristic that can be included in at least one implementation mode of the present invention. The phrase "in a preferred implementation mode" that appears in different places in this specification does not necessarily refer to the same embodiment, nor is it an embodiment that is separate from or mutually exclusive of other embodiments.

[0044] Furthermore, the present invention is described in detail in conjunction with the schematic diagrams. When describing the embodiments of the present invention in detail, for the convenience of explanation, the cross-sectional views showing the device structure will be enlarged locally out of the general proportion, and the schematic diagrams are only examples and should not limit the scope of protection of the present invention herein. In addition, in actual production, three-dimensional spatial dimensions including length, width and depth should be included.

[0045] Please refer to the attached Figures 1 to 3 、 Figure 11 As shown, this embodiment provides a test device for animal experiments in brain science based on optogenetic technology, including:

[0046] A main body 10, on the upper end of which a spectrometer 11 is fixedly installed, and two groups of covers 13 are movably inserted on the upper end of the main body 10, and replacement blocks 14 are movably inserted on both groups of covers 13;

[0047] An odor component 20, which is arranged on the upper end of the main body 10;

[0048] The hilly component 30 includes six groups of rubber skins 31, and the six groups of rubber skins 31 are fixedly connected to the lower end of the inner cavity of the main body 10;

[0049] The labyrinth component 40 includes a partition plate 41. A lifting plate 42 is slidably connected to the lower end of the inner cavity of the main body 10 near the partition plate 41, and a replacement plate 49 is fixedly connected to the lower end of the inner cavity of the main body 10 between adjacent partition plates 41;

[0050] Among them, the replacement block 14 is used to replace different functional modules. The rubber skin 31 rises or falls along the inner cavity of the main body 10 to simulate hills or deep pits. The lifting plate 42 is used to change the path of the partition plate 41, and the replacement plate 49 is used to simulate different road surfaces.

[0051] It should be noted that the main body 10 is made of a transparent material, such as PVC / PP / glass, and is preferably made of PP plastic material in this embodiment, aiming to facilitate the observation of the state of the organism;

[0052] A sealing strip is provided at the part where the cover 13 contacts the main body 10 to prevent the odor from leaking out and affecting the surrounding environment. Handles are provided on both the cover 13 and the replacement block 14 for easy opening and replacement;

[0053] The six groups of rubber skins 31 are cross-distributed to simulate the uneven ground and improve the effect of diversity detection.

[0054] Preferably, to ensure the normal use of the device, multiple installation slots should be provided above the main body 10, aiming to enable different functional modules (such as an air-conditioning module, a pressure module) to be installed on the main body 10 according to requirements, improve the diversity during the detection of the main body 10, and improve the integrity of the data.

[0055] In this embodiment, during the test, the spectrometer 11 operates to conduct optogenetic experiments on the organism. At the same time, the replacement block 14 can be replaced with different functional modules according to requirements. When the odor component 20 is connected to the cover 13 instead of the replacement block 14, the odor component 20 operates to blow different aerosols into the main body 10, facilitating the detection of the state of the organism. At the same time, the hilly component 30 controls the rubber skin 31 to rise or sink to form a hilly or deep-pit ground surface, so that the reaction of the organism to different ground surfaces can be observed. When the organism enters the labyrinth component 40, the labyrinth component 40 controls the lifting plate 42 to rise, changing the maze path composed of the partition plates 41, thereby forming a diversified maze to test the coping ability of the organism, and different replacement plates 49 with ground surface characteristics can be placed on the maze path, and then the reaction of the organism to different ground surface characteristics can be observed.

[0056] Secondly, please refer to again Figures 1 to 4, the lower end of the spectrometer 11 penetrates through the main body 10 and is fixedly connected to an optical fiber 12, and the lower end of the optical fiber 12 is connected to the head of the test animal, aiming to use the optical fiber 12 for optogenetic testing. The upper end of the main body 10 is fixedly installed with a lamp control board 15, the lamp control board 15 is electrically connected to an LED lamp 16, the LED lamp 16 is movably inserted into the cover 13, and the lower end of the LED lamp 16 extends into the inner cavity of the main body 10;

[0057] Two through grooves are provided at the upper end of each group of covers 13, and both the LED lamp 16 and the replacement block 14 are movably inserted into the through grooves. At both sides of the through grooves at the upper end of the cover 13, magnetic attraction plates 17 are fixedly connected, and the upper ends of the magnetic attraction plates 17 are in contact connection with the outer sides of the replacement block 14 and the LED lamp 16.

[0058] It should be noted that the optical fiber 12 has a Y-shaped structure, and its lower end is divided into two ends and connected to the head of the test animal;

[0059] The replacement block 14 can be disassembled according to requirements and replaced with modules with different functions to achieve diversified detection of the device. And a sealing strip is provided at the edge of the through groove at the upper end of the cover 13 to improve the sealing effect;

[0060] Magnetic attraction components adapted to the magnetic attraction plates 17 are provided at the lower ends of both the LED lamp 16 and the replacement block 14 to improve the connection stability.

[0061] In this embodiment, during use, the spectrometer 11 transmits optical information into the brain of the organism through the optical fiber 12 for optogenetic experiments. During the experiment, the replacement block 14 can be replaced with modules with different functions to achieve test experiments under multiple conditions and multiple positions. When testing different light intensities, the color temperature and brightness of the LED lamp 16 can be switched through the lamp control board 15 to achieve diversified detection, and the LED lamp 16 is fixed by the magnetic attraction plate 17 to improve the connection stability.

[0062] Secondly, please refer to again Figures 4 to 6 , the odor component 20 includes an air pump 21, the air pump 21 is fixedly connected to the upper end of the main body 10, the outlet of the air pump 21 is communicated with a blowing head 22, the blowing head 22 is movably inserted into the through groove at the upper end of the cover 13, and the upper end of the main body 10 is fixedly connected to an air inlet box 23, and the air inlet box 23 is communicated with the inlet of the air pump 21;

[0063] The odor component 20 further includes a placement rack 24. The placement rack 24 is movably inserted into the upper end of the inner cavity of the air inlet box 23. A limiting tube 25 is fixedly connected to the upper end of the inner cavity of the placement rack 24. A lifting rod 26 is slidably connected to the inner cavity of the limiting tube 25. The lower end of the lifting rod 26 extends out of the limiting tube 25 and is fixedly connected to a pressing plate 27. The pressing plate 27 is movably sleeved in the inner cavity of the placement rack 24. The upper end of the placement rack 24 is rotatably connected to a lead screw 28 through a ball bearing. The upper end of the lead screw 28 extends out of the placement rack 24, and the outer circle of the lower end of the lead screw 28 is threadedly connected to the inner cavity of the lifting rod 26.

[0064] It should be noted that a non-slip wheel is fixedly connected to the part of the upper end of the lead screw 28 that extends out of the placement rack 24, aiming to drive the rotation of the lead screw 28 by rotating the non-slip wheel;

[0065] A non-slip layer is provided at the lower end of the inner cavity of the placement rack 24, and a non-slip layer is also provided at the lower end of the pressing plate 27, aiming to place the components that release gas in the inner cavity of the placement rack 24, input the released gas into the main body 10 through the air pump 21, and be able to control the released amount according to requirements, improving the accuracy;

[0066] An inlet is opened on the front surface of the air inlet box 23, so that air can enter the air inlet box 23 through the inlet. A filter screen is provided at the inlet to prevent foreign impurities from entering the air inlet box 23. And a sealing strip is provided at the part where the upper end of the air inlet box 23 is connected to the placement rack 24, aiming to improve the sealing effect.

[0067] Preferably, to ensure that during the gas release process, the released gas will not leak outside the air inlet box 23, a one-way valve can be installed at the inlet of the air inlet box 23, so that the outside gas can only enter the air inlet box 23 through the one-way valve, and the gas in the air inlet box 23 will not leak, avoiding excessive gas leakage and affecting the surrounding environment.

[0068] In this embodiment, during the test, when the blowing head 22 replaces the replacement block 14, the experimenter can put the sources that release different gases into the placement rack 24, and then control the rotation of the lead screw 28 to make the lifting rod 26 descend in the limiting tube 25, thereby pushing the pressing plate 27 to descend to clamp and fix the source object. Then, the placement rack 24 is put into the air inlet box 23, and the air pump 21 operates to blow the gas in the air inlet box 23 into the main body 10 through the blowing head 22, simulating different environments and observing the reactions of organisms to different odors.

[0069] Secondly, please refer to again Figures 7 to 9, the hilly component 30 further includes a driving rod 32. The driving rod 32 is fixedly connected to the lower end of the rubber skin 31, and the lower end of the driving rod 32 is slidably connected to the lower end of the inner cavity of the main body 10. A spring 33 is movably sleeved on the outer circle of the driving rod 32. The lower end of the spring 33 is fixedly connected to the lower end of the inner cavity of the main body 10. A pull rope 34 is fixedly connected to the part of the lower end of the driving rod 32 extending into the inner cavity of the main body 10. The other end of the pull rope 34 penetrates through the main body 10 and extends out of the main body 10. A winding wheel 35 is rotatably connected to the lower end of the outer side of the main body 10. One end of the pull rope 34 extending out of the main body 10 is fixedly connected to the inside of the winding wheel 35;

[0070] A convex plate is fixedly connected to the outer circle of the upper end of the driving rod 32, and the upper end of the spring 33 is fixedly connected to the lower end of the convex plate, while the lower end of the spring 33 is fixedly connected to the lower end of the inner cavity of the main body 10, and the driving rod 32 is movably sleeved in the inner circle of the spring 33.

[0071] It should be noted that the rotating shaft connecting the winding wheel 35 and the main body 10 is a self-locking rotating shaft, and the unlocking method is to pull the winding wheel 35 outwards, aiming to fix the winding wheel 35 after pulling the pull rope 34 to contract, so as to prevent the pull rope 34 from automatically resetting and affecting the pulling effect on the rubber skin 31.

[0072] Preferably, to improve the simulation effect, structures such as convex points, grooves, patterns, etc. can be set on the outer surface of the rubber skin 31, thereby improving the authenticity.

[0073] In this embodiment, during the test, rotate the winding wheel 35 to control the tightening or release of the pull rope 34, and then control the lifting and lowering of the driving rod 32 at the lower end of the inner cavity of the main body 10. When the pull rope 34 is tightened, the driving rod 32 descends, and the rubber skin 31 sinks accordingly, simulating a deep pit terrain. When the pull rope 34 is released, the driving rod 32 is pushed up by the spring 33, and the rubber skin 31 rises accordingly, simulating a hilly terrain. At the same time, the setting of the spring 33 can provide a restoring elastic force for the rubber skin 31 when the pull rope 34 is released, ensuring that the rubber skin 31 can smoothly return to the initial state and prepare for the next test.

[0074] Please refer to again Figures 10 to 13 , the maze component 40 further includes four groups of air bags 43. The four groups of air bags 43 are fixedly connected to the bottom end of the inner cavity of the main body 10, and the upper ends of the four groups of air bags 43 are respectively fixedly connected to the lower ends of the four groups of lifting plates 42. The lower ends of the four groups of air bags 43 are all communicated with air pipes 44. The other ends of the four groups of air pipes 44 all extend out of the main body 10, and the parts of the four groups of air pipes 44 extending out of the main body 10 are all communicated with valves 45. The inlets and outlets of the four groups of valves 45 are all communicated with shunt pipes 46, and the middle of the shunt pipe 46 is communicated with an inlet and outlet pipe 47;

[0075] An empty groove is provided at the lower end of the inner cavity of the main body 10 at the position between adjacent partition plates 41, and an adsorption plate 48 is fixedly connected in the empty groove, and the replacement plate 49 is in contact connection with the upper end of the adsorption plate 48;

[0076] Both the adsorption plate 48 and the magnetic attraction plate 17 are magnetic attraction elements, aiming to fix the replacement plate 49 and the replacement block 14 by magnetic force.

[0077] It should be noted that the airbag 43 is made of rubber with a high elastic coefficient, and a connecting rope is provided at the part where the upper end of the airbag 43 contacts the lower end of the lifting plate 42, aiming to enable the lifting plate 42 to descend when the airbag 43 contracts;

[0078] The replacement plate 49 can be made into different shapes according to requirements, such as wavy or mirror-shaped, aiming to improve the diversification of detection.

[0079] Preferably, to ensure the normal operation of the device, the end of the access pipe 47 should be externally connected to a gas pump, and the gas pump should be able to switch between forward and reverse operations, so that the airbag 43 can be inflated through the access pipe 47 and the air pipe 44, and the airbag 43 can also be contracted by pumping air;

[0080] In addition, to ensure the diversification of detection, a certain lifting plate 42 can be controlled to rise and fall according to requirements to change the path of the partition plate 41. At the same time, the height of the lifting plate 42 rising and falling can also be controlled to form a semi-blocking structure, further improving the diversification of detection.

[0081] In this embodiment, when a biological enters the maze formed by the partition plates 41, the external gas pump is used to blow air through the access pipe 47. The gas enters the air pipe 44 through the valve 45, and then the airbag 43 is inflated or deflated, thereby controlling the lifting of the lifting plate 42. When the airbag 43 is inflated, the airbag 43 expands and pushes the lifting plate 42 to rise. After the lifting plate 42 rises, it will change the maze path composed of the partition plates 41, thereby forming a diversified maze to test the coping ability of the biological. When it is necessary to make the lifting plate 42 descend, the external gas pump is used to pump air to make the airbag 43 contract, and then the lifting plate 42 is pulled down through the connecting rope to restore the initial state of the maze path. And the opening and closing of a certain air pipe 44 can be controlled through the valve 45, thereby realizing the individual control of a certain lifting plate 42, improving the diversification and practicability of the test.

[0082] The working principle of the present invention is as follows: Put the organism into the main body 10. Subsequently, the spectrometer 11 transmits optical information into the brain of the organism through the optical fiber 12 for optogenetic experiments. During the experiment, the replacement block 14 can be replaced with modules of different functions. When testing different light intensities, the color temperature and brightness of the LED lamp 16 can be switched through the lamp control board 15 to achieve diversified detection. If the blowing head 22 replaces the replacement block 14, the experimenter can put the source releasing different gases into the placement rack 24, and then put the placement rack 24 into the air inlet box 23. The air pump 21 operates, and the gas in the air inlet box 23 is blown into the main body 10 through the blowing head 22 to simulate different environments and observe the organism's reaction to different odors. Moreover, the winding wheel 35 can be rotated to control the tightening or release of the pull rope 34, thereby controlling the lifting of the driving rod 32 at the lower end of the inner cavity of the main body 10. When the pull rope 34 is tightened, the driving rod 32 descends, and the rubber skin 31 sinks accordingly, simulating a deep pit terrain. When the pull rope 34 is released, the driving rod 32 is pushed upward by the spring 33, and the rubber skin 31 rises accordingly, simulating a hilly terrain, so as to observe the organism's reaction and coping ability to different terrains. When the organism enters the maze formed by the partition plate 41, air is blown through the external gas pump and the inlet and outlet pipe 47. The gas enters the trachea 44 through the valve 45, and then the airbag 43 is inflated or deflated, thereby controlling the lifting of the lifting plate 42. After the lifting plate 42 rises, it will change the maze path composed of the partition plate 41, thereby forming a diversified maze to test the organism's coping ability.

[0083] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art in this technical field, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention. The structures, devices, and operation methods not specifically described and explained in the present invention, unless otherwise specified and limited, are implemented according to the conventional means in the art.

Claims

1. A test device for animal experiments in brain science based on optogenetic technology, characterized in that: Comprising: A main body (10), on the upper end of which a spectrometer (11) is fixedly installed, and two covers (13) are movably inserted into the upper end of the main body (10), and replacement blocks (14) are movably inserted into both of the two covers (13); An odor component (20), which is arranged on the upper end of the main body (10); A hilly component (30), which includes six rubber skins (31), and the six rubber skins (31) are fixedly connected to the lower end of the inner cavity of the main body (10); A maze component (40), which includes a partition plate (41), a lifting plate (42) is slidably connected to the position near the partition plate (41) at the lower end of the inner cavity of the main body (10), and a replacement plate (49) is fixedly connected to the position between adjacent partition plates (41) at the lower end of the inner cavity of the main body (10); Wherein, the replacement block (14) is used to replace different functional modules, the rubber skin (31) rises or falls along the inner cavity of the main body (10) to simulate hills or deep pits, the lifting plate (42) is used to change the path of the partition plate (41), and the replacement plate (49) is used to simulate different road surfaces; The odor component (20) includes an air pump (21), the air pump (21) is fixedly connected to the upper end of the main body (10), the outlet of the air pump (21) is communicated with a blowing head (22), the blowing head (22) is movably inserted into a through groove at the upper end of the cover (13), and an air inlet box (23) is fixedly connected to the upper end of the main body (10), and the air inlet box (23) is communicated with the inlet of the air pump (21); The odor component (20) further includes a placement rack (24), the placement rack (24) is movably inserted into the upper end of the inner cavity of the air inlet box (23), a limiting tube (25) is fixedly connected to the upper end of the inner cavity of the placement rack (24), a lifting rod (26) is slidably connected to the inner cavity of the limiting tube (25), the lower end of the lifting rod (26) extends out of the limiting tube (25) and is fixedly connected to a pressing plate (27), the pressing plate (27) is movably sleeved in the inner cavity of the placement rack (24), a lead screw (28) is rotatably connected to the upper end of the placement rack (24), the upper end of the lead screw (28) extends out of the placement rack (24), and the outer ring of the lower end of the lead screw (28) is threadedly connected to the inner cavity of the lifting rod (26); The hilly component (30) further includes a driving rod (32). The driving rod (32) is fixedly connected to the lower end of the rubber skin (31), and the lower end of the driving rod (32) is slidably connected to the lower end of the inner cavity of the main body (10). An outer ring of the driving rod (32) is movably sleeved with a spring (33). A lower end of the spring (33) is fixedly connected to the lower end of the inner cavity of the main body (10). A part of the driving rod (32) extending into the inner cavity of the main body (10) at the lower end is fixedly connected with a pulling rope (34). The other end of the pulling rope (34) penetrates through the main body (10) and extends out of the main body (10). A lower end of the outer side of the main body (10) is rotatably connected with a winding wheel (35). One end of the pulling rope (34) extending out of the main body (10) is fixedly connected inside the winding wheel (35). The maze component (40) further includes four groups of air bags (43). The four groups of air bags (43) are fixedly connected to the bottom end of the inner cavity of the main body (10), and upper ends of the four groups of air bags (43) are respectively fixedly connected to lower ends of four groups of lifting plates (42). Lower ends of the four groups of air bags (43) are all communicated with air pipes (44). The other ends of the four groups of air pipes (44) all extend out of the main body (10), and parts of the four groups of air pipes (44) extending out of the main body (10) are all communicated with valves (45). Entrance and exit parts of the four groups of valves (45) are all communicated with shunt pipes (46). A middle part of the shunt pipe (46) is communicated with an access pipe (47).

2. The test device for brain science animal experiments based on optogenetic technology according to claim 1, wherein: A lower end of the spectrometer (11) penetrates through the main body (10) and is fixedly connected with an optical fiber (12). An upper end of the main body (10) is fixedly installed with a lamp control board (15). The lamp control board (15) is electrically connected with an LED lamp (16). The LED lamp (16) is movably inserted on the cover (13), and a lower end of the LED lamp (16) extends into the inner cavity of the main body (10).

3. The test device for brain science animal experiments based on optogenetic technology according to claim 2, characterized in that: Two through grooves are formed in an upper end of each group of covers (13), and the LED lamp (16) and the replacement block (14) are both movably inserted in the through grooves. Magnet adsorption plates (17) are fixedly connected to both sides of the through grooves at the upper end of the cover (13). Upper ends of the magnet adsorption plates (17) are in contact connection with the outer sides of the replacement block (14) and the LED lamp (16).

4. The test device for brain science animal experiments based on optogenetic technology according to claim 1, characterized in that: An outer ring of an upper end of the driving rod (32) is fixedly connected with a convex plate, and an upper end of the spring (33) is fixedly connected to a lower end of the convex plate. A lower end of the spring (33) is fixedly connected to the lower end of the inner cavity of the main body (10), and the driving rod (32) is movably sleeved in an inner ring of the spring (33).

5. The test device for brain science animal experiments based on optogenetic technology according to claim 3, characterized in that: An empty groove is formed at a position between adjacent partition plates (41) at the lower end of the inner cavity of the main body (10), and an adsorption plate (48) is fixedly connected in the empty groove. A replacement plate (49) is in contact connection with an upper end of the adsorption plate (48).

6. The test device for brain science animal experiments based on optogenetic technology according to claim 5, wherein: Both the adsorption plate (48) and the magnet adsorption plate (17) are magnetic adsorption elements.

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