Sampling device for calf diarrhea pathogen detection and detection method

Abstract

The invention discloses a sampling device for calf diarrhea pathogen detection. The sampling device comprises a sampling tube, an adjusting tube, a sampling film sleeve and an extraction assembly, the far end of the sampling tube is fixedly connected with the adjusting tube; the hollow section in the sampling tube comprises a rectangle and a circle which are communicated; telescopic cavities are formed in the upper side and the lower side of the near-end section of the sampling tube; the sampling tube is sleeved with the sampling film sleeve; the adjusting pipe is rotationally connected with the extraction assembly; the extraction assembly has a negative pressure function, so that the sampling film sleeve is sucked into the sampling pipe under the action of negative pressure; a pair of liquid conveying holes are formed in the sampling pipe, and the input ends of the liquid conveying holes penetrate into the adjusting pipe and penetrate out of the outer wall of the adjusting pipe; the output end of the infusion hole penetrates through the telescopic cavity; the device further comprises a pair of plugging assemblies and a connecting pipe. The pair of plugging assemblies is arranged in the telescopic cavity; each plugging assembly is fixedly connected with the output end of the infusion hole; two ends of the connecting pipe are fixedly connected with plugging assemblies.

Description

Technical Field

[0001] The present invention relates to the field of calf diarrhea sampling devices, and specifically to a sampling device and detection method for detecting calf diarrhea pathogens. Background Art

[0002] CN114129192B discloses a rapid sampling device for detecting calf diarrhea pathogens. The specific method of forming a sealed capsule during sampling is as follows: "The sampler twists the handle, causing the inner tube to rotate along with the handle. The inner tube rotates within the composite tube through a rotating sleeve. During the rotation of the inner tube, since the composite tube is in a state of being compressed by the calf's rectum, there is resistance between the composite tube and the calf's rectum. The rotation speed of the inner tube will be greater than that of the composite tube, creating a speed difference. When the inner tube rotates, the twisting resistance of the inner tube to the tubular membrane tube is increased through the tightening plate, and the tubular membrane tube at the front end of the inner tube is kinked to form a sealed capsule, thereby sealing and storing the sampled feces in the sealed capsule."

[0003] The rectum of a calf is very fragile. Utilizing the resistance of the inner wall of the calf's rectum to reduce the rotation speed of the composite tube will undoubtedly cause harm to the calf's rectum, making the calf feel uncomfortable and causing the calf to make various movements, thereby affecting the smooth progress of sampling.

[0004] Since the composite tube and the inner tube can rotate within the rectum, it can be known that the composite tube and the inner tube are made of hard materials. When the cow feels pain during the rotation of the composite tube, it will make various movements, and the hard end of the composite tube is also likely to cause harm to the calf's rectum.

[0005] A section of the calf's rectum near the anus needs to adapt to changes in abdominal pressure and defecation movements, and there is a natural bend. Therefore, the depth to which the composite tube and the inner tube can be inserted into the rectum is limited, and it is impossible to ensure that enough fecal samples can be obtained. For this reason, the present invention provides a sampling device and detection method for detecting calf diarrhea pathogens. Summary of the Invention

[0006] The purpose of the present invention is to provide a sampling device for detecting calf diarrhea pathogens to solve the problem of easily hurting the calf's rectum during sampling as mentioned in the above background art.

[0007] To achieve the above purpose, the present invention provides the following technical solutions: A sampling device for detecting calf diarrhea pathogens includes a sampling tube, an adjusting tube, a sampling membrane sleeve, and an extraction assembly; the distal end of the sampling tube is fixedly connected to the adjusting tube, and the hollow cross-section inside the sampling tube includes a communicating rectangle and a circle; the hollow cross-section of the sampling tube near the proximal end is rectangular, and the hollow cross-section near the distal end is circular; telescopic cavities are provided on the upper and lower sides of the proximal cross-section of the sampling tube with a rectangular hollow. The sampling membrane sleeve is sleeved outside the sampling tube; the adjusting tube is rotatably connected to the extraction component; the extraction component has a negative pressure function to suck the sampling membrane sleeve into the sampling tube under the action of negative pressure; a pair of infusion holes are provided inside the sampling tube, and the input ends of the infusion holes penetrate into the inside of the adjusting tube and penetrate out from the outer wall of the adjusting tube; the output ends of the infusion holes penetrate into the telescopic cavity; It further includes a pair of plugging components and a connecting pipe; the pair of plugging components are arranged in the telescopic cavity; each plugging component is fixedly connected to the output end of the infusion hole; both ends of the connecting pipe are fixedly connected to the plugging components; the plugging components have a telescopic function, and when the pair of plugging components are heated to a specified temperature, they start to stretch towards each other, otherwise the plugging components contract; to flatten the sampling membrane sleeve located in the through hole with a rectangular cross-section.

[0008] Preferably, a fixing ring is fixedly connected to the outer wall of the adjusting tube; the rear end of the sampling membrane sleeve is an open section; the open section is detachably connected to the side of the fixing ring away from the extraction component; the front end of the sampling membrane sleeve is a closed end, located at the proximal end of the sampling tube, and is used to bring feces into the sampling tube.

[0009] Preferably, the sampling tube is a flexible hose with a proximal end being arc-shaped; a flexible heat insulation strip is arranged between the opposite side walls of the plugging component and the connecting pipe, and the heat insulation strip is fixedly connected to the plugging component and the connecting pipe; a heat insulation layer is pasted on the inner wall of the sampling tube between the heat insulation strip and the adjusting tube; the adjusting tube is a rigid tube; the sampling membrane sleeve is made of a flexible material; the connecting pipe is a hose.

[0010] Preferably, the extraction component includes a rotating pipe fitting, a piston pipe, a piston part and a closing plate; both ends of the rotating pipe fitting are respectively rotatably connected to the adjusting tube and the piston pipe; the piston part is slidably connected inside the piston pipe; the closing plate is fixedly connected to the inner wall of the piston pipe and is located at the end of the piston pipe close to the rotating pipe fitting; the upper side of the closing plate is lower than the center of the piston pipe; a pair of symmetric ventilation holes are provided on the inner wall of the piston pipe inside the closing plate.

[0011] Preferably, the rotating pipe fitting includes a rotating tube, a rotating plate, a cross bar and a pair of plugging plates; both ends of the rotating tube are respectively rotatably connected to the adjusting tube and the piston pipe; the rotating plate is fixedly connected to the inner wall of the rotating tube close to the end of the piston pipe, and the lower side exceeds the center of the rotating tube and also exceeds the upper side of the closing plate; the cross bar is fixedly connected to the rotating plate; a pair of plugging plates are fixedly connected to the two ends of the cross bar perpendicular to the rotating tube; the plugging plates correspond to the pair of ventilation holes.

[0012] Preferably, the rotating pipe fitting further includes a positioning block; the positioning block is fixedly connected to the side of the closing plate close to the rotating plate; the positioning block is lower than the upper side of the closing plate.

[0013] Preferably, each plugging component includes a telescopic rod, a heating pipe, and a pressing plate; a pair of pressing plates are located on the upper and lower sides of a rectangular through-hole in cross-section; both ends of each telescopic rod are fixedly connected to the corresponding telescopic cavity and the pressing plate; a pair of heating pipes are fixedly connected to the same side of the telescopic rod for telescoping with the telescopic rod; both ends of the heating pipe are respectively fixedly connected to the connecting pipe and the output end of the infusion hole; the middle of the connecting pipe is fixedly connected to the side of the rectangular through-hole.

[0014] Preferably, the telescopic rod is made of shape memory alloy; the deformation temperature of the shape memory alloy is set to 50 - 60 °C, preferably 50 °C.

[0015] Preferably, a hole cap is inserted into the input end of one of the infusion holes; an injection hose is fixedly connected to the input end of the other infusion hole; a plurality of ventilation holes are also provided on the adjusting pipe, and the ventilation holes communicate with the side wall of the input end of the other infusion hole.

[0016] A detection method for a sampling device for detecting calf diarrhea pathogens is also provided, including the following steps: S1. First, put the sampling membrane sleeve on the sampling tube; rotate the rotating pipe fitting so that the plugging plate blocks the ventilation hole; insert the hole cap into the input end of the infusion hole on the lower side of the adjusting pipe. S2. Insert the proximal end of the sampling tube into the calf rectum. S3. During the insertion process, pull the piston part outwards; the negative pressure generated by the piston part in the piston tube sucks the sampling membrane sleeve and feces into the sampling tube. S4. When the piston part moves outwards to be close to the outer end of the piston tube, stop inserting the sampling tube into the rectum; rotate the rotating pipe fitting in the reverse direction so that the plugging plate is disengaged from the ventilation hole; then push the piston part in the reverse direction, and the gas in the piston tube is discharged through the ventilation hole; and block the ventilation hole again; then continue to pull the piston part outwards, and repeat this way, so as to continuously suck the sampling membrane sleeve into the sampling tube to collect more feces.

[0017] S5. After sampling, inject hot water at 50 - 60 °C, preferably 50 °C, into the injection hose; the hot water flows through the infusion hole to the heating pipe and heats the telescopic rod; when the pair of telescopic rods are heated to the specified temperature, they start to stretch towards each other, thereby driving the pair of pressing plates to move towards each other to flatten the sampling membrane sleeve located between the pair of pressing plates. S6. Withdraw the sampling tube from the calf rectum; then remove the hole cap, inject cold water into the injection hose again to cool the telescopic rod, so that the telescopic rod contracts back into the telescopic cavity; then remove the sampling membrane sleeve from the fixing ring and draw out the sampling membrane sleeve in the sampling tube; sampling is completed. S7. Finally, inject gas into the injection hose to discharge the residual liquid in the injection hose, infusion hole, heating pipe, and connecting pipe.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows: The structure of the present invention is simple, the operation is convenient, and the sampling process is safe. The sampling tube with both flexibility and toughness can bend along with the bending state of the bovine rectum when inserted into the bovine rectum. Coupled with the arc-shaped proximal end, the sampling tube will not cause harm to the bovine rectum when sampling, reducing the pain of the cow. More sampling tubes can also be inserted into the bovine rectum to collect more fecal samples. After the sampling tube is withdrawn, the sampling membrane sleeve can be removed from the sampling tube, and the structure of the present invention can be reused.

[0019] During the process of inserting the sampling tube into the bovine rectum, negative pressure is generated by pulling the piston member outwards, so that the sampling membrane sleeve and feces are sucked into the sampling tube together. Under the action of negative pressure, the feces will not flow out of the sampling tube.

[0020] By using the telescopic rod made of shape memory alloy, after heating, in cooperation with the extrusion plate, the sampling membrane sleeve between the extrusion plates is flattened to prevent the feces from flowing out during the process of withdrawing the sampling tube. Hot water or cold water is input into the heating tube through the infusion hole to control the temperature increase or decrease of the telescopic rod, so that the telescopic rod stretches or contracts. Since the infusion hole is arranged inside the sampling tube, the hot water flows between the infusion hole and the heating tube, and is insulated by the sampling tube, and the hot water will not contact the rectum of the calf, avoiding harm to the rectum of the calf. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is a schematic structural diagram of the present invention; Figure 2 is a schematic sectional view of the present invention; Figure 3 is of the present invention Figure 2 sectional schematic view of the adjusting tube 13 at A in; Figure 4 is a disassembled structural diagram of the rotating pipe fitting, piston pipe and piston pipe and a sectional view of the structure at the piston pipe of the present invention; Figure 5 is a disassembled structural diagram of the piston pipe and the rotating plate of the present invention; Figure 6 is a sectional view of the structure of the proximal end hollow of the sampling tube of the present invention; Figure 7 is of the present invention Figure 6 sectional schematic view of the structure of the plugging component at B in; Figure 8 is a sectional view of the connection between the plugging component and the telescopic cavity of the present invention; Figure 9 is a sectional view of the state after the telescopic plate is stretched of the present invention; Figure 10 is a schematic diagram of the position of the present invention inserted into the rectum of a calf; Figure 11 It is a cross-sectional view of the heat insulation layer in the sampling tube of the present invention; Figure 12 It is a cross-sectional view of the connection between the connecting pipe and the thermal insulation strip of the present invention.

[0022] In the figure: 11 infusion hole, 12 sampling tube, 121 telescopic cavity, 13 adjusting tube, 131 fixing ring, 21 sampling membrane sleeve, 3 extraction assembly, 31 rotating pipe fitting, 311 rotating tube, 312 rotating plate, 313 cross rod, 314 blocking plate, 32 piston tube, 321 vent hole, 33 piston part, 34 closing plate, 35 positioning block, 4 blocking assembly, 41 telescopic rod, 42 heating tube, 43 extrusion plate, 5 connecting tube, 61 hole cap, 62 injection hose, 63 air vent, 7 insulation strip, 8 insulation layer. DETAILED DESCRIPTION

[0023] Example 1

[0024] See also Figures 1 - 10 The present invention provides a technical solution: Figures 1 - 3 As shown, a sampling device for detecting calf diarrhea pathogens includes a sampling tube 12, an adjusting tube 13, a sampling membrane sleeve 21 and an extraction component 3. The far end of the sampling tube 12 is fixedly connected to the adjusting tube 13, and the sampling tube 12 and the adjusting tube 13 are hollowed out and communicated with each other. The hollowed-out cross-section inside the sampling tube 12 includes a communicating rectangle and a circle; the hollowed-out cross-section near the proximal end of the sampling tube 12 is a rectangle, and the hollowed-out cross-section near the distal end is a circle. The upper and lower sides of the rectangular hollowed-out cross-section of the proximal end of the sampling tube 12 are provided with telescopic cavities 121.

[0025] The outer wall of the regulating tube 13 is fixedly connected with a fixing ring 131. The sampling membrane sleeve 21 is sleeved on the outside of the sampling tube 12, and the rear end of the sampling membrane sleeve 21 is an open section, and is detachably connected to the side of the fixing ring 131 away from the extraction component 3; the front end of the sampling membrane sleeve 21 is a closed end, located at the proximal end of the sampling tube 12, and is used to bring feces into the sampling tube 12. The regulating tube 13 is rotatably connected to the extraction component 3. The extraction component 3 has a negative pressure function, so that the front end of the sampling membrane sleeve 21 is sucked into the sampling tube 12 under the action of negative pressure.

[0026] A pair of infusion holes 11 are provided inside the sampling tube 12 . The input ends of the infusion holes 11 penetrate into the inside of the regulating tube 13 and pass out from the outer wall of the regulating tube 13 . The output ends of the infusion holes 11 penetrate into the telescopic cavity 121 .

[0027] The present invention also includes a pair of blocking components 4 and a connecting tube 5. The pair of blocking components 4 is arranged in the telescopic cavity 121. Each blocking component 4 is fixedly connected to the output end of the infusion hole 11. The two ends of the connecting tube 5 are fixedly connected to the blocking components 4, and the connecting tube 5 is attached to the side of the through hole with a rectangular cross section. The infusion hole 11 is used to transport fluid to the blocking components 4. The fluid is hot water, and the temperature of the hot water is 50-60°C.

[0028] The plugging assembly 4 has a telescopic function. When the fluid contacts the plugging assembly 4, a pair of plugging assemblies 4 are heated to a specified temperature and then start to stretch towards each other to crush the sampling membrane sleeve 21 located in the through hole with a rectangular cross-section.

[0029] The sampling tube 12 is a flexible tube with a certain toughness and good heat insulation effect. The proximal end of the sampling tube 12 is arc-shaped. The adjusting tube 13 is a rigid tube. The sampling membrane sleeve 21 is made of a flexible material. The connecting tube 5 is a flexible tube.

[0030] As Figures 3 - 5 shown, the extraction assembly 3 includes a rotating pipe fitting 31, a piston tube 32, a piston member 33, and a closing plate 34. One end of the rotating pipe fitting 31 is rotatably connected to the adjusting tube 13. The rotating pipe fitting 31 includes a rotating tube 311, a rotating plate 312, a cross bar 313, and a pair of plugging plates 314. Specifically, one end of the rotating tube 311 is rotatably connected to the adjusting tube 13, and the other end is rotatably connected to the piston tube 32. The closing plate 34 is fixedly connected to the inner wall of the piston tube 32, and the closing plate 34 is arranged at the end of the piston tube 32 close to the rotating pipe fitting 31. The upper side of the closing plate 34 is lower than the center of the piston tube 32. A pair of symmetric ventilation holes 321 are opened on the inner wall of the piston tube 32 inside the closing plate 34.

[0031] The rotating plate 312 is fixedly connected to the inner wall of the rotating tube 311 close to the end of the piston tube 32. The lower side of the rotating plate 312 exceeds the center of the rotating tube 311 and also exceeds the upper side of the closing plate 34. The cross bar 313 is fixedly connected to the rotating plate 312 and coincides with the center of the rotating tube 311. A pair of plugging plates 314 are fixedly connected to the two vertical ends of the cross bar 313 perpendicular to the rotating tube 311. The plugging plates 314 correspond to the pair of ventilation holes 321.

[0032] The rotating pipe fitting 31 further includes a positioning block 35. The positioning block 35 is fixedly connected to the side of the closing plate 34 close to the rotating plate 312. The positioning block 35 is lower than the upper side of the closing plate 34 and is used to position the position of the rotating plate 312. When the lower side of the rotating plate 312 is located on the upper side of the positioning block 35, the rotating plate 312 closes the upper side of the closing plate 34, so that the inside of the rotating tube 311 and the piston tube 32 are not connected. On the contrary, when the rotating plate 312 rotates away from the positioning block 35, the inside of the rotating tube 311 and the piston tube 32 start to communicate; when the plugging plates 314 block the ventilation holes 321, the communicating area between the inside of the rotating tube 311 and the piston tube 32 is the largest. At this time, when the piston member 33 is pulled outwards, a negative pressure will be generated, and the sampling membrane sleeve 21 can be sucked into the sampling tube 12 through the negative pressure and further move in the sampling tube 12.

[0033] As Figure 2 and Figures 6 - 10As shown in the figure, each plugging component 4 includes a telescopic rod 41, a heating tube 42, and a pressing plate 43. A pair of pressing plates 43 are located on the upper and lower sides of a rectangular through-hole in cross-section. Both ends of each telescopic rod 41 are fixedly connected to the corresponding telescopic cavity 121 and the pressing plate 43. A pair of heating tubes 42 are fixedly connected to the same side of the telescopic rod 41, and the length of the heating tube 42 is the same as that of the telescopic rod 41, and is used to expand and contract along with the telescopic rod 41. Both ends of the heating tube 42 are respectively fixedly connected to the connecting pipe 5 and the output end of the infusion hole 11. So that the inside of a pair of infusion holes 11, a pair of heating tubes 42, and the connecting pipe 5 are interconnected. The middle of the connecting pipe 5 is fixedly connected to the side surface of the rectangular through-hole to limit the position of the connecting pipe 5.

[0034] The telescopic rod 41 is made of shape memory alloy. The deformation temperature of the shape memory alloy is set to 50 degrees. When the temperature is below 50 degrees, the telescopic rod 41 is folded in a Z shape in the telescopic cavity 121. When hot water is input into the heating tube 42 through the infusion hole 11, the temperature of the telescopic rod 41 rises to 50 degrees and then begins to deform, stretching towards the rectangular through-hole in cross-section; thereby driving the pressing plate 43 to press the sampling membrane sleeve 21; the temperature of the hot water is between 50 - 60 °C, which can also ensure the safety of the operator.

[0035] As Figure 3 shown in the figure, a hole cap 61 is inserted into the input end of the infusion hole 11 on the lower side of the adjusting tube 13, and the hole cap 61 is fixedly connected to the outer wall of the adjusting tube 13 by a rope. An injection hose 62 is fixedly connected to the input end of the infusion hole 11 on the upper side of the adjusting tube 13, which is convenient for injecting hot water into the infusion hole 11 through an injection needle. A plurality of ventilation holes 63 are also opened on the adjusting tube 13, and the ventilation holes 63 communicate to the vicinity of the input end of the upper infusion hole 11; when the input end of the infusion hole 11 on the lower side of the adjusting tube 13 is inserted with the hole cap 61, when injecting hot water into the input end of the infusion hole 11 on the upper side of the adjusting tube 13, it is used to discharge the gas in the infusion hole 11, the heating tube 42, and the connecting pipe 5 through the ventilation holes 63, so that the heating tube 42 can be filled with hot water to ensure the heating effect on the telescopic rod 41.

[0036] A detection method for a sampling device for detecting calf diarrhea pathogens includes the following steps: S1. First, put the sampling membrane sleeve 21 on the sampling tube 12; and make the closed end of the sampling membrane sleeve 21 be located at the proximal end of the sampling tube 12. Rotate the rotating pipe fitting 31 so that the plugging plate 314 blocks the ventilation hole 321. Insert the hole cap 61 into the input end of the infusion hole 11 on the lower side of the adjusting tube 13.

[0037] S2. Slowly insert the proximal end of the sampling tube 12 into the rectum of the calf. The sampling tube 12 has a certain toughness and can change its curvature along with the shape of the rectum, thereby reducing the burden and damage to the calf's rectum.

[0038] S3. During the insertion process, pull the piston member 33 outwards. The negative pressure generated by the piston member 33 within the piston tube 32 sucks the closed end of the sampling membrane sleeve 21 into the sampling tube 12; as the sampling tube 12 continues to be inserted, the sampling membrane sleeve 21 will continuously bring the feces in the rectum into the sampling tube 12.

[0039] S4. When the piston member 33 moves outwards to be close to the outer end of the piston tube 32, stop inserting the sampling tube 12 into the rectum. Rotate the rotating pipe fitting 31 in the reverse direction so that the lower side of the rotating tube 311 abuts against the positioning block 35, closing the hollow of the closing rod 34. At this time, the blocking plate 314 disengages from the ventilation hole 321. Then push the piston member 33 in the reverse direction, and the gas within the piston tube 32 is discharged through the ventilation hole 321. Rotate the rotating pipe fitting 31 again so that the blocking plate 314 blocks the ventilation hole 321. Then continue to pull the piston member 33 outwards, and repeat this process, thereby continuously sucking the sampling membrane sleeve 21 into the sampling tube 12 to collect more feces.

[0040] S5. After the sampling is completed, inject hot water at 50 degrees into the injection hose 62 through an injection tube (not shown in the figure). The hot water flows through the infusion hole 11 to the heating tube 42 to heat the telescopic rod 41. After a pair of telescopic rods 41 are heated to the specified temperature, they start to stretch towards each other, thereby driving a pair of pressing plates 43 to move towards each other to crush the sampling membrane sleeve 21 between the pair of pressing plates 43, preventing feces from flowing out. There is a certain distance between the infusion hole 11 and the inner wall of the sampling tube 12, and the volume within the sampling tube 12 accounts for a relatively small proportion. Therefore, when the hot water flows in the infusion hole 11 and the heating tube 42, the temperature increase of the inner wall of the sampling tube 12 is limited. This avoids the influence of excessive temperature within the sampling tube 12 on the collected feces.

[0041] S6. Withdraw the sampling tube 12 from the rectum of the calf. Then remove the hole cap 61, and inject cold water into the injection hose 62 again through the injection tube. With the push of the cold water, the hot water is discharged, and at the same time, the telescopic rod 41 is cooled, causing the telescopic rod 41 to contract back into the telescopic cavity 121. Then remove the open end of the sampling membrane sleeve 21 from the fixing ring 131, and withdraw the sampling membrane sleeve 21 within the sampling tube 12. The sampling is completed.

[0042] S7. Finally, inject gas into the injection hose 62 through the injection tube to discharge the residual liquid in the injection hose 62, the infusion hole 11, the heating tube 42, and the connecting tube 5.

[0043] Embodiment 2

[0044] As Figures 11 - 12As shown, on the basis of Embodiment 1, a heat insulation strip 7 with a certain flexibility is provided between the side walls of the extrusion plate 43 of the sampling tube 12 and the connecting pipe 5 which are opposite to each other. The heat insulation strip 7 is fixedly connected to the extrusion plate 43 and the connecting pipe 5. When the extrusion plate 43 moves, it can move together with the connecting pipe 5. When hot water flows through the connecting pipe 5, the heat is insulated to prevent the heat from being transferred to the rectangular hollow, thus damaging the fecal sample.

[0045] A heat insulation layer 8 is pasted on the inner wall of the sampling tube 12 between the heat insulation strip 7 and the adjusting tube 13.

[0046] The heat insulation layer 8 and the sampling tube 12 are used for double-layer heat insulation to protect the feces in the sampling tube 12. The heat insulation strip 7 and the heat insulation layer 8 are prepared from heat insulation materials such as aerogel felt, heat insulation paper or other heat insulation materials.

Claims

1. A sampling device for detecting pathogens of calf diarrhea, characterized in that: The sampling tube (12) comprises a sampling tube (12), a regulating tube (13), a sampling membrane sleeve (21) and an extraction assembly (3); the distal end of the sampling tube (12) is fixedly connected to the regulating tube (13); the hollowed-out cross section inside the sampling tube (12) comprises a communicating rectangle and a circle; the hollowed-out cross section near the proximal end of the sampling tube (12) is a rectangle, and the hollowed-out cross section near the distal end is a circle; the proximal end of the sampling tube (12) has a rectangular hollowed-out cross section with telescopic cavities (121) on both upper and lower sides. The sampling membrane sleeve (21) is sleeved on the outside of the sampling tube (12); the regulating tube (13) is rotatably connected to the extraction component (3); the extraction component (3) has a negative pressure function, so that the sampling membrane sleeve (21) is sucked into the sampling tube (12) under the action of negative pressure; a pair of infusion holes (11) are opened inside the sampling tube (12), and the input ends of the infusion holes (11) penetrate into the inside of the regulating tube (13) and pass out from the outer wall of the regulating tube (13); the output ends of the infusion holes (11) penetrate into the telescopic cavity (121); It also comprises a pair of blocking components (4) and a connecting tube (5); the pair of blocking components (4) are arranged in the telescopic cavity (121); each blocking component (4) is fixedly connected to the output end of the infusion hole (11); both ends of the connecting tube (5) are fixedly connected to the blocking components (4); the blocking components (4) have a telescopic function, when the pair of blocking components (4) are heated to a specified temperature, they begin to stretch towards each other, and vice versa, the blocking components (4) contract, so as to compress the sampling membrane sleeve (21) located in the through hole with a rectangular cross-section.

2. A sampling device for detecting calf diarrhea pathogens according to claim 1, characterized in that: The outer wall of the regulating tube (13) is fixedly connected to a fixing ring (131); the rear end of the sampling membrane sleeve (21) is an open section; the open section is detachably connected to a side of the fixing ring (131) away from the extraction component (3); the front end of the sampling membrane sleeve (21) is a closed end, which is located at the proximal end of the sampling tube (12) and is used to carry feces into the sampling tube (12).

3. A sampling device for detecting calf diarrhea pathogens according to claim 1, characterized in that: The sampling tube (12) is a flexible hose with an arc-shaped proximal end; a flexible heat-insulating strip (7) is provided between the opposite side walls of the plugging component (4) and the connecting tube (5), and the heat-insulating strip (7) is fixedly connected to the plugging component (4) and the connecting tube (5); a heat-insulating layer (8) is adhered to the inner wall of the sampling tube (12) between the heat-insulating strip (7) and the regulating tube (13); the regulating tube (13) is a hard tube; the sampling membrane sleeve (21) is made of a flexible material; and the connecting tube (5) is a hose.

4. A sampling device for detecting calf diarrhea pathogens according to claim 1, characterized in that: The extraction assembly (3) comprises a rotating tube (31), a piston tube (32), a piston member (33) and a closing plate (34); the two ends of the rotating tube (31) are respectively rotatably connected to the regulating tube (13) and the piston tube (32); the piston member (33) is slidably connected in the piston tube (32); the closing plate (34) is fixedly connected to the inner wall of the piston tube (32) and is located at the end of the piston tube (32) close to the rotating tube (31); the upper side of the closing plate (34) is lower than the center of the piston tube (32); and a pair of symmetrical vent holes (321) are opened on the inner wall of the piston tube (32) located inside the closing plate (34).

5. A sampling device for detecting calf diarrhea pathogens according to claim 4, characterized in that: The rotating tube member (31) comprises a rotating tube (311), a rotating plate (312), a cross rod (313) and a pair of blocking plates (314); the two ends of the rotating tube (311) are respectively rotatably connected to the regulating tube (13) and the piston tube (32); the rotating plate (312) is fixedly connected to the inner wall of the rotating tube (311) near the end of the piston tube (32), and the lower side exceeds the center of the rotating tube (311) and the upper side of the sealing plate (34); the cross rod (313) is fixedly connected to the rotating plate (312); the two ends of the cross rod (313) and the rotating tube (311) are fixedly connected to a pair of blocking plates (314); the blocking plates (314) correspond to a pair of vent holes (321).

6. A sampling device for detecting calf diarrhea pathogens according to claim 5, characterized in that: The rotating pipe (31) further comprises a positioning block (35); the positioning block (35) is fixedly connected to the side surface of the closing plate (34) close to the rotating plate (312); the positioning block (35) is lower than the upper side of the closing plate (34).

7. A sampling device for detecting calf diarrhea pathogens according to claim 1, characterized in that: Each blocking assembly (4) comprises a telescopic rod (41), a heating tube (42) and an extrusion plate (43); a pair of extrusion plates (43) are located at the upper and lower sides of a through hole with a rectangular cross section; both ends of each telescopic rod (41) are fixedly connected to the corresponding telescopic cavity (121) and the extrusion plate (43); a pair of heating tubes (42) are fixedly connected to the same side of the telescopic rod (41) and are used to follow the telescopic rod (41) to be extended and retracted; both ends of the heating tube (42) are respectively fixedly connected to the connecting tube (5) and the output end of the infusion hole (11); and the middle part of the connecting tube (5) is fixedly connected to the side of the rectangular through hole.

8. A sampling device for detecting calf diarrhea pathogens according to claim 7, characterized in that: The telescopic rod (41) is made of a memory alloy; the deformation temperature of the memory alloy is set to 50-60°C.

9. A sampling device for detecting calf diarrhea pathogens according to claim 1, characterized in that: A hole cap (61) is inserted into the input end of one of the infusion holes (11); an injection hose (62) is fixedly connected to the input end of the other infusion hole (11); and a plurality of air holes (63) are also formed on the regulating tube (13), the air holes (63) being connected to the side wall of the input end of the other infusion hole (11).

10. A detection method for a sampling device for detecting pathogens of calf diarrhea, characterized in that: The detection method is based on a sampling device for detecting calf diarrhea pathogens according to any one of claims 1 to 9, comprising the following steps: S1. First, put the sampling membrane sleeve (21) on the sampling tube (12); rotate the rotating tube (31) so that the sealing plate (314) blocks the vent (321); insert the hole cap (61) into the input end of the infusion hole (11) on the lower side of the regulating tube (13); S2, inserting the proximal end of the sampling tube (12) into the rectum of the calf; S3. During the insertion process, the piston member (33) is pulled outward; the sampling membrane sleeve (21) and feces are sucked into the sampling tube (12) through the negative pressure generated by the piston member (33) in the piston tube (32); S4. When the piston member (33) moves outward to the outer end of the piston tube (32), stop inserting the sampling tube (12) into the rectum; rotate the rotating tube member (31) in the opposite direction so that the blocking plate (314) is out of contact with the vent (321); then push the piston member (33) in the opposite direction so that the gas in the piston tube (32) is discharged through the vent (321); and the vent (321) is blocked again; then continue to pull the piston member (33) outward, and repeat this process so that the sampling membrane sleeve (21) is continuously sucked into the sampling tube (12) to collect more feces; S5. After the sampling is completed, hot water at 50° C. to 60° C. is injected into the injection hose (62); the hot water flows to the heating tube (42) through the infusion hole (11) and heats the telescopic rod (41); after the pair of telescopic rods (41) are heated to a specified temperature, they begin to stretch toward each other, thereby driving the pair of extrusion plates (43) to move toward each other to compress the sampling membrane sleeve (21) located between the pair of extrusion plates (43); S6, extract the sampling tube (12) from the rectum of the calf; then remove the hole cap (61), and inject cold water into the injection hose (62) again to cool the telescopic rod (41), so that the telescopic rod (41) shrinks back into the telescopic cavity (121); then remove the sampling membrane sleeve (21) from the fixing ring (131), and extract the sampling membrane sleeve (21) in the sampling tube (12); complete the sampling; S7. Finally, gas is injected into the injection hose (62) to discharge the residual liquid in the injection hose (62), the infusion hole (11), the heating tube (42) and the connecting tube (5).

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